CN217717465U - Optical microscope multi-sample clamping device - Google Patents
Optical microscope multi-sample clamping device Download PDFInfo
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- CN217717465U CN217717465U CN202220357278.4U CN202220357278U CN217717465U CN 217717465 U CN217717465 U CN 217717465U CN 202220357278 U CN202220357278 U CN 202220357278U CN 217717465 U CN217717465 U CN 217717465U
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Abstract
The utility model discloses a multi-sample clamping device of an optical microscope, which comprises a multi-sample clamping frame; one end of the multi-sample holding frame is provided with a plurality of sample carrying platforms which are used for fixing a plurality of biological samples at the same time, and each sample carrying platform can be independently placed into a sample cavity of an imaging system; the other end of the sample clamping frame is connected with a sample rotating shaft, and the multi-sample clamping frame is driven to rotate through the rotation of the sample rotating shaft and is used for the rotation of a specific sample or the switching between biological samples of different sample carrying platforms; the device also comprises a support arm support and a rotating part, wherein the support arm support is used for being fixed with the displacement table, and the rotating part is used for rotating the knob to drive the multi-sample clamping frame to rotate so as to realize switching among different biological samples; the utility model provides a many samples clamping device can compatible multiple biological sample, uses the scene extensive, can help the researcher to improve formation of image efficiency effectively, can realize the micro-formation of image of high flux under many samples formation of image application scenes.
Description
Technical Field
The utility model belongs to sample clamping device field, more specifically relates to an optical microscope multiple sample clamping device.
Background
Light sheet illumination microscopy is currently gaining wide attention from various researchers in the international world as an emerging leading edge imaging method in the twenty-first century. The light sheet microscopic imaging system has the characteristics of high axial resolution, higher flux, low photobleaching and the like, and is widely concerned in the field of biomedical imaging. Compared with the traditional fluorescence microscopic imaging, the light sheet fluorescence microscopic system adopts an additional illumination light path to generate a layer of extremely thin laser light sheet. When the sample is placed in the light sheet working area, only the plane through which the thin light sheet passes can excite a fluorescence signal, and the fluorescence signal is collected by a wide-field collecting light path which is orthogonal to the illumination light path to form an image. A series of section fluorescence image sequences can be generated by rapidly scanning a sample or an optical sheet in the detection optical path direction, namely the z direction, and the three-dimensional structure of the sample is reconstructed by stacking.
In the light sheet microscopic imaging system, a sample needs to be fixed in the imaging system through a clamping device, and the clamping device serves as a part of the imaging system, so that an illumination light path and a detection light path are not shielded on the premise of stably clamping the sample. In general, whether in vivo or ex vivo imaging, the sample must be fixed in an optically clear medium or immersed in an index-matching solution, or aberrations and distortions can be introduced.
Currently, two types of sample fixing methods are mainly known according to the size of a sample, and a living sample represented by zebra fish is mainly prepared by embedding the sample in an FEP tube or fixing the sample on a glass slide by using agarose; the main means of ex vivo sampling, represented by mouse brain, is to hold the sample against a fixed wall by means of a fastening screw. When the traditional single-sample clamping technology faces a multi-sample large-scale imaging scene, each sample needs to be sequentially loaded, repeated sample loading can reduce imaging efficiency and limit imaging flux, and for different biological samples, due to different volumes and the like, a single clamping piece cannot be compatible with biological samples of various sizes, so that the imaging efficiency is low; in the prior art, only one sample can be clamped at each time, the clamping of a plurality of single samples cannot be accommodated and used simultaneously due to the limitation of the volume of a sample cavity, an optical microscope multi-sample clamping device is urgently needed, and switching among different biological samples can be realized, so that the optical microscope multi-sample clamping device is suitable for a multi-sample large-scale high-efficiency imaging scene.
SUMMERY OF THE UTILITY MODEL
Defect more than to prior art or improvement demand, the utility model provides an optical microscope many samples clamping device, its aim at is through being equipped with two at least many samples holding frame that carry the appearance platform, solves prior art can only centre gripping a sample, the technical problem of formation of image inefficiency from this.
To achieve the above object, according to one aspect of the present invention, there is provided a multi-sample holding device for an optical microscope, comprising a multi-sample holding rack 3; one end of the multi-sample holding frame 3 is provided with at least two sample carrying tables for fixing a plurality of biological samples at the same time, and each sample carrying table can be independently placed into a sample cavity of an imaging system; the other end is connected with the sample rotating shaft 210, and the multi-sample clamping frame is driven to rotate by the rotation of the sample rotating shaft, so that the special sample can rotate or the biological samples on different sample carrying platforms can be switched.
Preferably, in the multi-sample holding device for an optical microscope, the sample stage is an L-shaped sample stage 33, and a screw hole is formed in a short edge of the L-shaped sample stage; the multi-sample holding frame 3 further comprises a first main body, wherein the first main body is positioned at the bottom of the sample carrying platform, matched with the L-shaped sample carrying platform 33 and used for fixing biological samples with different sizes.
Preferably, the sample holder is a flat sample holder 34 for attaching a biological sample.
Preferably, in the optical microscope multi-sample clamping device, the multi-sample clamping rack 3 is provided with 2-6 sample loading platforms which are uniformly distributed; the other end is provided with a hollow cavity structure for connecting the sample rotating shaft 210.
Preferably, in the optical microscope multi-sample clamping device, the multi-sample clamping frame 3 is provided with 4-6 sample carrying tables, and the other end of the multi-sample clamping frame is also provided with a screw hole for connecting with a sample rotating shaft or a screw thread for connecting with the sample rotating shaft.
Preferably, the optical microscope multi-sample holding device further comprises a rotating part 2, wherein the rotating part 2 comprises a second body, a rotating shaft 25, a first gear 28, a second gear 212, and a sample rotating shaft 210;
the top of the second main body is provided with a reference line 23 and a knob 26; the rotating shaft 25 is inserted into the second body, the bottom of the rotating shaft is connected with the first gear 28, the axial directions of the rotating shaft and the first gear are overlapped, and the top of the rotating shaft is fixed with the knob 26; the second gear 212 is located on the second body side wall, is engaged with the first gear 28, and has an axial direction perpendicular to the axial direction of the first gear 28; one end of the sample rotation shaft 210 is connected to the second gear 212, and the axial direction thereof coincides with the axial direction of the second gear 212, and the other end thereof is engaged with the multi-sample holder 3 to fix the multi-sample holder.
Preferably, the optical microscope multi-sample holding device further comprises a support arm frame 1; one end of the support arm frame is connected with the rotating part 2 and used for fixedly supporting the rotating part 2, and the other end of the support arm frame is used for being fixed with the displacement table.
Preferably, the optical microscope multi-sample clamping device is provided with scale marks 27 on the periphery of the knob 26, which cooperate with the reference line 23 to control the rotation angle, and the rotation shaft 25 is rotated to drive the multi-sample clamping frame 3 to rotate through the first gear 28 and the second gear 212, so as to switch between multiple samples.
Preferably, the optical microscope multi-sample holding device is characterized in that the support arm frame 1, the rotating part 2 and the multi-sample holding frame 3 are detachably connected; the second body of the rotating part 2 is also provided with a first through hole 22 and a first magnet 21 at the top.
Preferably, the optical microscope multi-sample holding device, the support arm frame 1 thereof, comprises a disc base 11 and a support arm 12; the disc base 11 is provided with a screw hole 13 and is fixed with the displacement table through a screw; one end of the supporting arm 12 is connected with the disc base, the axial directions of the supporting arm and the disc base are vertical, and the other end of the supporting arm is provided with a second magnet 14 and a vertical rod 15; the second magnet 14 corresponds to the first magnet 21, and the vertical rod is engaged with the first through hole 22 to fix the rotating part 2.
Generally, through the utility model discloses the above technical scheme who thinks compares with prior art, because the utility model provides an optical microscope many samples clamping device can gain following beneficial effect:
the utility model provides an optical microscope multi-sample clamping device, which comprises a multi-sample clamping frame, wherein one end of the multi-sample clamping frame is provided with at least two sample carrying tables, especially 2-6 sample carrying tables, which can be used for fixing different biological samples at the same time; the other end is connected with the sample rotating shaft, the multi-sample clamping frame is driven to rotate through the rotation of the sample rotating shaft, the rotation of a specific sample or the switching between biological samples of different sample carrying platforms can be realized, the repeated replacement of the sample is avoided, the time and the labor are saved, and the imaging efficiency is improved.
The utility model provides an optical microscope multiple sample clamping device, its multiple sample clamping frame still include the first main part, are located carry the appearance platform bottom, and carry the appearance platform and can be set as L type and carry the appearance platform, and the minor face is equipped with the screw, can fix the sample of different sizes through the nut revolves the screw depth and cooperates with first main part lateral wall; or the sample carrying platform is set as a plane sample carrying platform, and different biological samples are fixed in a pasting mode.
Drawings
FIG. 1 is a schematic view of a multi-specimen holder 3 of the sample holding apparatus for an optical microscope in example 1;
FIG. 2 is a schematic view of a multi-specimen holder 3 of the sample-holding apparatus for an optical microscope in example 3;
FIG. 3 is an exploded view of the rotating portion of the optical microscope sample holder;
21-a first magnet, 22-a first through hole, 23-a reference line, 24-a second through hole, 25-a rotating shaft, 26-a knob, 27-a scale mark, 28-a first gear, 29-a third through hole, 210-a sample rotating shaft, 211-a screw hole and 212-a second gear; wherein, 1-a support arm frame, 2-a rotating part and 3-a multi-sample holding frame;
FIG. 4 is a schematic structural diagram of a support arm of the optical microscope sample holding device;
wherein, 11-disc base, 12-supporting arm, 13-screw hole, 14-second magnet, 15-vertical rod;
FIG. 5 is a schematic diagram of the overall structure of the optical microscope sample holding device;
wherein, 1-supporting arm support, 2-rotating part and 3-multi-sample holding frame.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. Furthermore, the technical features mentioned in the embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.
In the description of the present invention, it should be noted that the terms "front", "back", "left", "right", "upper", "lower", "vertical", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
The schematic diagram of the multi-sample clamping device for the optical microscope provided by the utility model is shown in fig. 1-3, and comprises a multi-sample clamping frame 3; one end of the multi-sample holding frame 3 is provided with at least two sample carrying tables for fixing a plurality of biological samples at the same time, and each sample carrying table can be independently placed into a sample cavity of an imaging system; the other end is connected with sample axis of rotation 210, through the rotation of sample axis of rotation drives the rotation of many sample holding frame for the rotation of specific sample or the switching between the different sample platform biological samples.
In some embodiments, the sample stage is an L-shaped sample stage 33, and the short side of the sample stage is provided with a screw hole; the multi-sample holding frame 3 further comprises a first main body, wherein the first main body is positioned at the bottom of the sample carrying platform, and the L-shaped sample carrying platform 33 is matched with the side wall of the first main body through the nut screwing depth and is used for fixing biological samples with different sizes.
In some embodiments, the sample carrier is a flat sample carrier 34 for attaching a biological sample.
Furthermore, the multi-sample holding frame 3 is provided with 2-6 sample carrying tables which are uniformly distributed, preferably 4-6 sample carrying tables for fixing a plurality of biological samples at the same time, so that the imaging efficiency is prevented from being reduced and the imaging flux is prevented from being limited due to repeated loading during multi-sample imaging; the other end is provided with a hollow inner cavity structure for connecting with the sample rotating shaft 210, and preferably, the other end is also provided with a screw hole connected with the sample rotating shaft through a jackscrew or a screw thread connected with the sample rotating shaft.
Further, the optical microscope multi-sample holding device further comprises a rotating part 2, wherein the rotating part 2 comprises a second body, a rotating shaft 25, a first gear 28, a second gear 212 and a sample rotating shaft 210;
the top of the second main body is provided with a reference line 23 and a knob 26; the rotating shaft 25 is vertically inserted into the second main body through a through hole, the bottom of the rotating shaft is connected with the first gear 28, the axial directions of the rotating shaft and the first gear are overlapped, and the top of the rotating shaft is fixed with the knob 26 through a jackscrew; the second gear 212 is located on the second body side wall, is engaged with the first gear 28, and has an axial direction perpendicular to the axial direction of the first gear 28; one end of the sample rotating shaft 210 is connected with the second gear 212, the axial direction of the sample rotating shaft coincides with the axial direction of the second gear 212, the other end of the sample rotating shaft is embedded with the multi-sample holding frame 3, and the rotating shaft 25 is rotated to drive the multi-sample holding frame 3 to rotate through the first gear 28 and the second gear 212, so that the rotation of a specific sample or the switching among multiple samples can be realized;
preferably, the knob 26 is provided with a graduation line 27 on the periphery thereof, and cooperates with the reference line 23 to control the rotation angle.
Furthermore, the rotating part 2 is provided with a first magnet 21, a first through hole 22 and a second through hole 24 on the left side of the top of the second main body; the second through hole 24 is used for inserting the rotating shaft 25; and a third through-hole 29 is formed at a lower end thereof for connection with the sample rotating shaft 210.
Further, the optical microscope multi-sample clamping device further comprises a support arm frame 1; one end of the support arm frame is connected with the rotating part 2 and used for fixedly supporting the rotating part 2, and the other end of the support arm frame is used for being fixed with a displacement table;
preferably, the support arm frame 1, the rotating part 2 and the multi-sample holding frame 3 are detachably connected.
Further, the support arm frame 1 comprises a disc base 11 and a support arm 12; the disc base 11 is provided with screw holes 13 for fixing with the displacement table through screws, and preferably four screw holes are formed; one end of the supporting arm 12 is connected with the disc base, the axial directions of the supporting arm and the disc base are vertical, the other end of the supporting arm is provided with an inwards concave table surface, the inwards concave table surface is provided with a second magnet 14 corresponding to the first magnet 21 in position, the supporting arm is further provided with a vertical rod 15, and the vertical rod is embedded with the first through hole 22 and matched with the magnet to fix the rotating part 2.
The following are examples:
example 1 optical microscope multiple sample holding device 1
The optical microscope multiple sample holding device has the overall structure as shown in fig. 5, and the multiple sample holding rack is as shown in fig. 1.
As shown in fig. 1, the multi-sample holding rack comprises a first main body, wherein one end of the first main body is provided with 4L-shaped sample carrying platforms which are uniformly distributed, the short edge of the first main body is provided with screw holes, and samples with different sizes can be fixed by matching the screwing depth of nuts with the side wall of the first main body; the other end is a hollow cavity structure and is provided with a screw hole for connecting with the sample rotating shaft 210 through a jackscrew;
the optical microscope multi-sample clamping frame 1 further comprises a supporting arm frame 1 and a rotating part 2;
the rotating part 2, which includes a second body, a rotating shaft 25, a knob 26, a first gear 28, a second gear 212, and a sample rotating shaft 210, as shown in fig. 3; wherein, the left side of the top of the second main body is provided with two first through holes 22, a second through hole 24, a first magnet 21 and a reference line 23, and the lower end is provided with a third through hole 29; the rotating shaft 25 is inserted into the second main body through the second through hole 24, the bottom of the rotating shaft is connected with the first gear 28, the top of the rotating shaft is connected with the knob 26, the periphery of the knob 26 is provided with scale marks 27 which are matched with the reference line 23 to control the rotating angle, and the rotation of the sample or the switching between samples is controlled by rotating the knob; the second gear 212 is positioned on the side wall of the second body and is embedded with the first gear 28; one end of the sample rotating shaft 210 is connected with the second gear 212, and the two are axially overlapped and connected with the second main body through the third through hole 29;
the support arm frame 1 comprises a disc base 11 and a support arm 12, wherein one end of the support arm is a concave step surface, and is provided with a second magnet 14 and two vertical rods 15, as shown in fig. 4, for being fixed with the rotating part 2; the other end is connected with the disc base and is fixed with the displacement table through a screw hole 13.
Example 2 optical microscope multiple sample holding device 2
The overall structure of the optical microscope multiple sample holding device is schematically shown in fig. 5, and the multiple sample holding rack is as follows.
The multi-sample clamping frame comprises a first main body, wherein 6L-shaped sample carrying platforms are arranged at one end of the first main body and are uniformly distributed, screw holes are formed in the short edge of the first main body, and samples with different sizes can be fixed through the matching of the screwing depth of a nut and the side wall of the first main body; the other end is a hollow cavity structure and is provided with a screw hole for connecting with the sample rotating shaft 210 through a jackscrew; the optical microscope multi-sample clamping device 2 further comprises a support arm frame 1 and a rotating part 2, and the rest is the same as that of the embodiment 1.
Example 3 optical microscope multiple sample holding device 3
The optical microscope multi-sample holding device is shown in fig. 5, and the multi-sample holding rack is shown in fig. 2.
As shown in fig. 2, one end of the multi-sample holding rack is provided with 5 plane sample-carrying platforms which are uniformly distributed and can fix different biological samples by means of pasting; the other end is of a hollow cavity structure and is provided with threads for connecting with the sample rotating shaft 210; the optical microscope multi-sample clamping device 3 further comprises a support arm frame 1 and a rotating part 2, and the rest is the same as that of the embodiment 1.
The utility model provides an optical microscope multiple sample clamping device uses with bread board, actuating lever, the cooperation of two dimension displacement platform, and 11 with four fix with screws of the disc base that the fixing device who has gripped the sample supported the arm to the displacement bench, and the bread board is fixed to the displacement bench, and multiple sample holding frame 3 is deep into imaging system's sample indoor. The light sheet can be projected on the sample from the left side and the right side of the multi-sample holding frame 3 to excite the fluorescence of the sample, and the objective lens collects the fluorescence generated by the excitation of the light sheet in the vertical direction of the light sheet and converges the fluorescence on detection devices such as a rear camera and the like to perform exposure imaging.
The bread board is used for fixing the whole device; the driving rod drives the whole device to vertically translate by inputting an electric signal, and when the polished section is fixed, the sample vertically moves to realize three-dimensional scanning of the polished section on the single sample; the top knob 26 of the rotating part is rotated, and the multi-sample clamping frame 3 is driven to rotate sequentially through the vertical rotating shaft 25, the first gear 28 and the second gear 212, so that free switching among multiple samples is realized; the rotation angle can be controlled according to the peripheral scale marks 27 of the knob and the reference line to realize the rotation of a specific sample, and the continuous switching among a plurality of samples can also be realized.
It will be understood by those skilled in the art that the foregoing is merely a preferred embodiment of the present invention, and is not intended to limit the invention to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A multi-sample holding device for an optical microscope, comprising a multi-sample holding rack (3); one end of the multi-sample clamping frame (3) is provided with at least two sample carrying tables for fixing a plurality of biological samples at the same time, and each sample carrying table can be independently placed into a sample cavity of an imaging system; the other end is connected with a sample rotating shaft (210), and the multi-sample clamping frame is driven to rotate through the rotation of the sample rotating shaft and is used for the rotation of a specific sample or the switching between biological samples of different sample carrying platforms.
2. The multi-sample holding device for optical microscope as claimed in claim 1, wherein the sample stage is an L-shaped sample stage, and the short side of the L-shaped sample stage is provided with a screw hole; the multi-sample clamping frame (3) further comprises a first main body, wherein the first main body is positioned at the bottom of the sample carrying platform, is matched with the L-shaped sample carrying platform and is used for fixing biological samples with different sizes.
3. The multi-sample holder of claim 1, wherein the sample stage is a flat sample stage for adhering a biological sample.
4. The optical microscope multi-sample holding device according to claim 1, wherein the multi-sample holding rack (3) is provided with 2-6 sample loading platforms and is distributed uniformly; the other end is provided with a hollow inner cavity structure for connecting a sample rotating shaft (210).
5. The optical microscope multiple specimen holding device according to claim 4, wherein the multiple specimen holding rack (3) is provided with 4-6 sample loading platforms, and the other end is further provided with a screw hole for connecting with the specimen rotating shaft or a screw thread for connecting with the specimen rotating shaft.
6. The optical microscope multiple sample holding device according to any one of claims 1 to 5, further comprising a rotating part (2), the rotating part (2) comprising a second body, a rotating shaft (25), a first gear (28), a second gear (212), and a sample rotating shaft (210);
the top of the second main body is provided with a reference line (23) and a knob (26); the rotating shaft (25) is inserted into the second body, the bottom of the rotating shaft is connected with the first gear (28), the rotating shaft and the first gear are overlapped in the axial direction, and the top of the rotating shaft is fixed with the knob (26); the second gear (212) is positioned on the second body side wall, is meshed with the first gear (28) and has an axial direction perpendicular to the axial direction of the first gear (28); one end of the sample rotating shaft (210) is connected with the second gear (212), the axial direction of the sample rotating shaft coincides with the axial direction of the second gear (212), and the other end of the sample rotating shaft is embedded with the multi-sample holding frame (3) and used for fixing the multi-sample holding frame.
7. The optical microscope multi-sample holding device according to claim 6, further comprising a support arm (1); one end of the support arm frame is connected with the rotating part (2) and used for fixedly supporting the rotating part (2), and the other end of the support arm frame is used for being fixed with the displacement table.
8. The optical microscope multi-sample holding device according to claim 7, wherein the knob (26) is provided with a graduation mark (27) on the periphery thereof, which cooperates with the reference line (23) to control the rotation angle.
9. The optical microscope multi-sample holding device according to claim 8, wherein the support arm (1), the rotating part (2) and the multi-sample holding rack (3) are detachably connected; the top of the second main body in the rotating part (2) is also provided with a first through hole (22) and a first magnet (21).
10. The optical microscope multi-sample holding device according to claim 9, wherein the support arm (1) comprises a disc base (11) and a support arm (12); the disc base (11) is provided with a screw hole (13) and is fixed with the displacement table through a screw; one end of the supporting arm (12) is connected with the disc base, the axial directions of the supporting arm and the disc base are vertical, and the other end of the supporting arm (12) is provided with a second magnet (14) and a vertical rod (15); the second magnet (14) corresponds to the first magnet (21) in position, and the vertical rod is embedded with the first through hole (22) and used for fixing the rotating part (2).
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