CN217304584U

CN217304584U - Even freezing preprocessing device of plant leaf

Info

Publication number: CN217304584U
Application number: CN202122584881.4U
Authority: CN
Inventors: 王兴国; 杨艳丽; 刘霞; 黄勋; 张哲�; 张雅敏; 王文萍
Original assignee: Yunnan Agricultural University
Current assignee: Yunnan Agricultural University
Priority date: 2021-10-27
Filing date: 2021-10-27
Publication date: 2022-08-26
Anticipated expiration: 2031-10-27

Abstract

The utility model relates to a plant leaf uniform freezing pretreatment device, which belongs to the technical field of plant leaf treatment, and comprises a base (1), a freezing tank (2), a tank cover (3), a freezing spoon (4) and a filter bag (10); the freezing tank (2) is of a double-layer structure and is placed on the base (1); the tank cover (3) is detachably covered on the freezing tank (2); the spoon body of the freezing spoon (4) is arranged in the freezing tank (2), and the spoon handle is leaked out of the tank cover (3). The utility model discloses can guarantee that the plant is at freezing in-process, it is freezing even, and can reduce the evaporation of liquid nitrogen.

Description

Even freezing preprocessing device of plant leaf

Technical Field

The utility model belongs to the technical field of the plant leaf is handled, specific theory relates to a plant leaf evenly freezes preprocessing device.

Background

Liquid nitrogen quick freezing blade in-process becomes the gaseous state from liquid, absorbs a large amount of heats, can be so that the moisture in the plant tissue is fixed, freezes the sample rapidly, can show the mechanical strength who improves plant tissue, not only can freeze the sample fast, does benefit to subsequent experimental sample's grinding and extraction moreover.

Liquid nitrogen plays a vital role in the process of preserving plant tissue samples, when carrying out related tests such as resistance related enzyme activity detection, RNA extraction, soluble sugar, soluble protein and malonaldehyde content measurement on a large scale, the plant samples need to be preserved at low temperature, the plant tissues need to be frozen by the liquid nitrogen before preservation, but unreasonable or insufficient utilization of the liquid nitrogen is a normal state, so that the waste of the liquid nitrogen is caused, and unnecessary potential safety hazards are also caused. For example, in the process of potato leaf refrigeration, liquid nitrogen is poured into a sterile self-sealing bag in which plant tissues are stored, so that the liquid nitrogen submerges plants, and the plants are stored after the liquid nitrogen is completely volatilized, which has the following problems: (1) the liquid nitrogen remaining when freezing the leaves is wasted; (2) liquid nitrogen is easy to spill when transferred to the sterile self-sealing bag by a liquid transfer device, so that operators are easy to be frostbitten by the liquid nitrogen; (3) the blades are usually vertically arranged, and the upper blades are exposed to the air firstly in the liquid nitrogen volatilization process, so that the freezing of the blades is not uniform; (4) liquid nitrogen is added into the bag, the ambient temperature is higher while the liquid nitrogen is contacted with a high-temperature sample, and the liquid nitrogen is volatilized rapidly, so that the conditions that the leaf blade is not frozen thoroughly and uniformly are caused.

In the current potato disease research, sampling and storage are necessary processes, good freezing leaves are very critical to scientific research, and the problem of rapidly, effectively, safely and uniformly freezing a large number of potato samples is a difficult problem.

Disclosure of Invention

In order to overcome the problem that exists among the background art, the utility model provides a plant leaf evenly freezes preprocessing device can guarantee that the plant is freezing in-process, and freezing even, and can reduce the waste of liquid nitrogen.

In order to achieve the above purpose, the utility model is realized by the following technical scheme:

the plant leaf freezing and uniformly pretreating device comprises a base, a freezing tank, a tank cover and a freezing spoon; the freezing tank is of a double-layer structure and is placed on the base; the cover can be detachably covered on the freezing tank; the spoon body of the freezing spoon is placed in the freezing tank, and the spoon handle is leaked out of the tank cover.

Preferably, the double-layer structure of the freezing tank comprises an outer protective layer and an inner silvered glass layer; the silver-plated glass layer is of a double-layer vacuum structure.

Preferably, the base is of a frustum structure with a groove formed in the upper end face; the bottom of the freezing tank is an arc-shaped bottom surface matched with the groove.

Preferably, the spoon body of the freezing spoon is provided with a filter hole; and scale marks are arranged in the freezing tank.

Preferably, a freezing spoon support is arranged in the freezing tank; the freezing spoon support comprises a support rod and an arc-shaped plate fixed at the upper end of the support rod; the radian of the arc-shaped plate is matched with the radian of the spoon body of the freezing spoon; the spoon body of the freezing spoon is placed on the arc-shaped plate.

Preferably, a filter bag is sleeved outside the freezing spoon, and a pulling rope is arranged at the bag opening of the filter bag; the filter bag is sleeved outside the spoon body of the freezing spoon, and the bag opening is fastened on the spoon handle through a drawing rope.

Preferably, the tank cover is divided into a left tank cover and a right tank cover which are mutually independent and are tangentially closed; and semicircular holes for the spoon handle to penetrate through are oppositely formed on the opposite surfaces of the left tank cover and the right tank cover.

Preferably, a safety exhaust pipe is arranged on the right tank cover; the upper end surface of the left can cover is provided with a handle.

Preferably, the side surfaces of the right tank cover and the left tank cover are respectively provided with a connecting plate and a connecting bolt vertically fixed on the lower end surface of the connecting plate; the outer wall of the freezing tank is provided with a fixing plate which is vertically opposite to the connecting plate; the fixing plate is provided with a connecting hole matched with the connecting bolt; the right tank cover and the left tank cover are detachably connected with the freezing tank through a fixing plate and a connecting bolt.

Preferably, the safety vent pipe comprises an outer rubber pipe layer and an inner vent pipe layer.

The utility model has the advantages that:

the utility model discloses can realize the cryopreservation of a large amount of potato blades, through setting up freezing spoon and filter bag, do benefit to the sample and evenly freeze in the liquid nitrogen with effective to can change the filter bag at any time, convenient and fast's while can also be with the blade restriction in the filter bag, can not be in the same place the sample of different processing. Through setting up safe blast pipe, can avoid the danger that liquid nitrogen volatilizees and cause. The utility model discloses simple structure, convenient to use, safety, it is respond well to preserve, and can effectively reduce the evaporation of liquid nitrogen.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

FIG. 2 is a right side plan view of FIG. 1;

FIG. 3 is a sectional view of the safety vent pipe of the present invention;

fig. 4 is a top view of the freezing spoon of the present invention;

in the figure, 1-base, 2-freezing tank, 3-tank cover, 4-freezing spoon, 5-external protective layer, 6-silvered glass layer, 7-filtering hole, 8-supporting rod, 9-arc plate, 10-filtering bag, 11-pulling rope, 12-left tank cover, 13-right tank cover, 14-semicircular hole, 15-safety exhaust pipe, 16-handle, 17-connecting plate, 18-connecting bolt, 19-fixing plate and 20-rubber tube.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the following will make a detailed description of a preferred embodiment of the present invention with reference to the accompanying drawings to facilitate understanding of the skilled person.

As shown in figures 1-4, the plant leaf uniform freezing pretreatment device comprises a base 1, a freezing tank 2, a tank cover 3 and a freezing spoon 4.

The tank wall of the freezing tank 2 is of a heat-preservation double-layer structure and comprises an outer protective layer 5 and an inner silver-plated glass layer 6; the silvered glass layer 6 is similar to the inner container of a thermos bottle and is of a double-layer vacuum structure, the external protective layer 5 can play a role in heat preservation, can prevent the silvered glass layer 6 from being damaged, and can be made of plastic materials.

The base 1 is a frustum structure with a groove formed in the upper end face, the frustum structure with the larger lower end and the smaller upper end can increase the stability of the base 1, and the bottom of the freezing tank 2 is an arc-shaped bottom face matched with the groove. When the refrigerator is used, the freezing tank 2 is placed in the groove, and the base 1 and the freezing tank 2 can be separated from each other, so that the stability of the freezing tank 2 can be guaranteed, and the refrigerator is convenient to process.

The cover 3 divide into left cover 12 and right cover 13 (the position term here is left, right, and the position of using fig. 2 is the standard, is the expression mode that adopts for the convenience of describing the utility model discloses a structure, the utility model discloses when in service, do not receive this position term restriction), left cover 12 and right cover 13 are tangent closed, close whole freezing jar 2 covers.

The right tank cover 13 is provided with a safety exhaust pipe 15, so that volatilized liquid nitrogen can be discharged in time, and the overhigh pressure in the freezing tank 1 is prevented; the outer parcel of safe blast pipe 15 has rubber tube 20, and when installing safe blast pipe 15, insert rubber tube 20 earlier, then with safe blast pipe 15 insert in rubber tube 20, rubber tube 20 has certain elasticity, plays the effect of fixed safe blast pipe.

The upper end face of the left can cover 12 is provided with a handle 16, which is convenient for opening the left can cover 12. By providing the left tank cover 12 and the right tank cover 13. Opposite surfaces of the left cover 12 and the right cover 13 are oppositely provided with semicircular holes 14 for the spoon handle to pass through, and the two semicircular holes 14 enclose a circular hole so that the spoon handle passes through. As a preferred technical scheme, lay a layer of elastic material on the inner wall of round hole to press from both sides tight spoon handle, prevent that freezing spoon 4 from rocking. Meanwhile, the can cover 3 is divided into two parts, so that one can cover can be opened at any time, and the spoon handle is still supported and is not easy to topple.

By setting the tank cover 3 as the left tank cover and the right tank cover: when the frozen sample is replaced, the cover is opened towards two sides at the same time, the frozen spoon can be quickly taken out, the frozen sample is placed into the sterile self-sealing bag for refrigeration, and a new sample is replaced at the same time; the freezing spoon is placed into the freezing tank after being loaded with a sample, and the left tank cover and the right tank cover are simultaneously closed to play a role in supporting the spoon handle.

The side surfaces of the left tank cover 12 and the right tank cover 13 are respectively provided with a connecting plate 17 and a connecting bolt 18 vertically fixed on the lower end surface of the connecting plate 17, and the outer wall of the freezing tank 2 is provided with a fixing plate 19 vertically opposite to the connecting plate 17; the fixing plate 19 is provided with a connecting hole matched with the connecting screw 18, and the left tank cover 12 and the right tank cover 13 are detachably connected with the freezing tank 2 through the fixing plate 19 and the connecting bolt 18 respectively.

A freezing spoon support is arranged in the freezing tank 2, the freezing spoon support comprises a support rod 8 and an arc-shaped plate 9 fixed to the upper end of the support rod 8, the radian of the arc-shaped plate 9 is matched with the radian of a spoon body of the freezing spoon 4, the spoon body of the freezing spoon 4 is placed on the arc-shaped plate 9, and a spoon handle is leaked out of the tank cover 3.

As a preferred technical scheme, the freezing tank 2 is internally provided with scale marks which can measure the liquid nitrogen in the tank.

The inner diameter of the freezing spoon 4 is slightly smaller than that of the freezing tank 2, a filtering hole 7 is formed in the spoon body of the freezing spoon 4, and liquid nitrogen can enter the freezing spoon due to the large aperture. The freezing spoon 4 is also sleeved with a filter bag 10, a bag mouth of the filter bag 10 is provided with a pulling rope 11, the filter bag 10 is sleeved outside the spoon body of the freezing spoon 4, and the bag mouth is fastened on the spoon handle through the pulling rope 11. The aperture of the filter hole 7 of the filter bag 10 is smaller than that of the filter hole of the freezing spoon 4, the filter bag 4 is arranged to limit the moving range of a sample in the filter bag and is positioned at the bottom of the freezing tank 2, the sample can be uniformly and effectively frozen in the freezing spoon 4, the filter bag 10 can be replaced at any time, and different samples can be frozen by replacing the filter bag. When the leaves are frozen, it is sometimes necessary to freeze smaller leaves, for example, before the potato sample is refrigerated, it is necessary to weigh and preserve 0.5g, and it may be necessary to cut the leaves to 0.5 g. The filter bag 10 prevents small plant tissues from entering the liquid nitrogen, thereby not only avoiding the liquid nitrogen pollution, but also ensuring the collection of samples.

Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention as defined by the appended claims.

Claims

1. The utility model provides a plant leaf evenly freezes preprocessing device which characterized in that: comprises a base (1), a freezing tank (2), a tank cover (3) and a freezing spoon (4); the freezing tank (2) is of a double-layer structure and is placed on the base (1); the tank cover (3) is detachably covered on the freezing tank (2); the spoon body of the freezing spoon (4) is arranged in the freezing tank (2), and the spoon handle is leaked out of the tank cover (3).

2. The plant leaf uniform freezing pretreatment device of claim 1, wherein: the double-layer structure of the freezing tank (2) comprises an outer protective layer (5) and an inner silver-plated glass layer (6); the silver-plated glass layer (6) is of a double-layer vacuum structure.

3. The plant leaf uniform freezing pretreatment device of claim 1, wherein: the base (1) is of a frustum structure with a groove formed in the upper end surface; the bottom of the freezing tank (2) is an arc bottom surface matched with the groove.

4. The plant leaf uniform freezing pretreatment device of claim 1, wherein: a filter hole (7) is formed in the body of the freezing spoon (4); and scale marks are arranged in the freezing tank (2).

5. The pre-treatment device for uniformly freezing plant leaves as claimed in any one of claims 1 to 4, wherein: a freezing spoon support is arranged in the freezing tank (2); the freezing spoon support comprises a support rod (8) and an arc-shaped plate (9) fixed at the upper end of the support rod (8); the radian of the arc-shaped plate (9) is matched with the radian of the spoon body of the freezing spoon (4); the spoon body of the freezing spoon (4) is placed on the arc-shaped plate (9).

6. The plant leaf uniform freezing pretreatment device of claim 1, wherein: a filter bag (10) is sleeved outside the freezing spoon (4), and a drawing rope (11) is arranged at the mouth of the filter bag (10); the filter bag (10) is sleeved outside the spoon body of the freezing spoon (4), and the bag mouth is fastened on the spoon handle through a pulling rope (11).

7. The plant leaf uniform freezing pretreatment device of claim 1, wherein: the tank cover (3) is divided into a left tank cover (12) and a right tank cover (13); the opposite surfaces of the left can cover (12) and the right can cover (13) are oppositely provided with semicircular holes (14) for the spoon handles to pass through.

8. The plant leaf uniform freezing pretreatment device of claim 7, wherein: a safety exhaust pipe (15) is arranged on the right tank cover (13); the upper end surface of the left tank cover (12) is provided with a handle (16).

9. The plant leaf uniform freezing pretreatment device according to claim 7 or 8, characterized in that: the side surfaces of the left tank cover (12) and the right tank cover (13) are respectively provided with a connecting plate (17) and a connecting bolt (18) vertically fixed on the lower end surface of the connecting plate (17); a fixing plate (19) which is vertically opposite to the connecting plate (17) is arranged on the outer wall of the freezing tank (2); the fixing plate (19) is provided with a connecting hole matched with the connecting bolt (18); the left tank cover (12) and the right tank cover (13) are detachably connected with the freezing tank (2) through a fixing plate (19) and a connecting bolt (18) respectively.

10. The plant leaf uniform freezing pretreatment device of claim 8, wherein: the safety exhaust pipe (15) is wrapped by a rubber pipe (20).