CN217132938U - Microscopic linear polarization fluorescence spectrum imaging measurement system - Google Patents
Microscopic linear polarization fluorescence spectrum imaging measurement system Download PDFInfo
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- CN217132938U CN217132938U CN202220379170.5U CN202220379170U CN217132938U CN 217132938 U CN217132938 U CN 217132938U CN 202220379170 U CN202220379170 U CN 202220379170U CN 217132938 U CN217132938 U CN 217132938U
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Abstract
The utility model belongs to sample optical detection field, a micro-linear polarization fluorescence spectrum imaging measurement system specifically says so. The method comprises the following steps: the device comprises a second reflecting mirror, a first reflecting objective, a second reflecting objective, a movable reflecting mirror, an 1/2 wave plate, a Glan prism, a high-pass filter and a first focusing mirror which are sequentially arranged in a line, and further comprises a light source generating system, a microscopic imaging system and a spectrum collecting system; the light source generating system is arranged on an incident light path of the second reflecting mirror; the microscopic imaging system is arranged on a reflection light path of the movable reflector; the spectrum acquisition system is arranged on a transmission light path of the first focusing mirror; and a space for placing the sample to be measured is arranged between the first reflection type objective lens and the second reflection type objective lens. The utility model discloses gather and the measuring scheme to the different position fluorescence in the sample area of being surveyed, make accurate measurement be surveyed sample linear polarization fluorescence spectrum and to be surveyed sample different positions's linear polarization fluorescence spectrum acquisition and become possible.
Description
Technical Field
The utility model belongs to sample optical detection field, a micro-linear polarization fluorescence spectrum imaging measurement system specifically says so.
Background
The linear polarization fluorescence spectrum measuring system is a device which utilizes linear polarization exciting light to excite a characteristic spectrum section of sample molecules and collects and displays linear polarization fluorescence radiated by a measured sample, and the measurement of the linear polarization fluorescence spectrum can assist in analyzing the chemical characteristics of the molecules.
When the linear polarization fluorescence spectrum measurement is performed, in order to complete excitation of the excitation light on the measured sample and collection of the radiation polarized fluorescence of the measured sample at the same side of the measured sample, a dichroic mirror is usually used to separate the linear polarization fluorescence from the excitation light, and the actual linear polarization fluorescence of the sample cannot be accurately collected due to different transmittances of the dichroic mirror on linear polarization light beams in different directions.
For a tested sample with uneven spatial distribution, particularly a diamond anvil cell high-pressure sample and a liquid nitrogen or liquid helium refrigerated low-temperature sample, linear polarization fluorescence radiated by different positions of the sample after being excited by linear polarization excitation light is different, so that accurate acquisition of linear polarization fluorescence spectrum information of the different positions of the sample is necessary, and no related achievement report capable of realizing the function exists at present.
SUMMERY OF THE UTILITY MODEL
In order to solve the above problem, the utility model provides a can accurately gather and measure by survey sample linear polarization fluorescence to gather and measured scheme to by survey sample district different positions fluorescence, make the accurate measurement by survey sample linear polarization fluorescence spectrum and to the collection of the linear polarization fluorescence spectrum of by survey sample different positions become possible.
An object of the utility model is to provide a linear polarization fluorescence spectrum imaging measurement system, the system is the matrix that will be surveyed the sample district division of sample and be a plurality of rows of a plurality of columns to excite the sample district that corresponding matrix element corresponds with the linear polarization exciting light, the linear polarization fluorescence of sample district radiation is collected by the spectrum appearance, and is right through the position that changes the surveyed sample the collection of the linear polarization fluorescence spectrum of the matrix element position that the sample district corresponds, thereby realizes the formation of image to the linear polarization fluorescence spectrum of the sample of being surveyed and measures.
The utility model discloses a realize that the technical scheme that above-mentioned purpose adopted is:
a microscopic linear polarized fluorescence spectral imaging measurement system, comprising: the device comprises a second reflecting mirror, a first reflecting objective, a second reflecting objective, a movable reflecting mirror, an 1/2 wave plate, a Glan prism, a high-pass filter and a first focusing mirror which are sequentially arranged in a line, and further comprises a light source generating system, a microscopic imaging system and a spectrum collecting system;
the light source generating system is arranged on an incident light path of the second reflecting mirror; the microscopic imaging system is arranged on a reflection light path of the movable reflector; the spectrum acquisition system is arranged on a transmission light path of the first focusing mirror; and a space for placing the tested sample is arranged between the first reflection type objective lens and the second reflection type objective lens.
The light source generation system includes: the laser device comprises a first reflector, a second reflector, a movable beam splitting sheet, a polarizing sheet and a first reflector, wherein the movable beam splitting sheet, the polarizing sheet and the first reflector are sequentially arranged in a line on an incident light path of the second reflector, and the laser device also comprises a laser light source arranged on the incident light path of the first reflector and a white light source arranged on the incident light path of the movable beam splitting sheet.
The polarizing plate is provided on a frame having a rotational degree of freedom about a laser propagation direction.
The microscopic imaging system comprises: the CCD camera is connected with the second computer through the second data transmission line, and the third reflector, the second focusing mirror and the CCD camera are sequentially arranged on a reflection light path of the movable reflector.
The microscopic imaging system comprises: the spectrometer comprises a light transmitting optical fiber input end, a light transmitting optical fiber, a spectrometer, a first data transmission line and a first computer which are connected in sequence, wherein the light transmitting optical fiber input end is arranged on a light transmitting path of a first focusing mirror.
The light transmission optical fiber input end is arranged on the lens bracket with the freedom of movement in the direction vertical to the propagation direction of the light path.
When the measured sample is subjected to microscopic imaging, the movable beam splitting sheet moves to a position between the polarizing sheet and the second reflecting mirror, the movable reflecting mirror is positioned between the second reflecting objective lens and the 1/2 wave plate, and white light emitted by the white light source sequentially passes through the movable beam splitting sheet, the second reflecting mirror and the second reflecting objective lens to illuminate the measured sample, so that the measured sample sequentially passes through the second reflecting objective lens and the movable reflecting mirror to be imaged in a microscopic imaging system.
When the spectral measurement is carried out on a sample to be measured, the movable beam splitting sheet and the movable reflector are respectively moved out of a line for arrangement, a natural excitation light beam emitted by the laser source sequentially passes through the reflector I, the polaroid, the reflector II, the reflective objective I and the focusing mirror I and irradiates on the sample to be measured, so that the sample to be measured is excited and then radiates linear polarization fluorescence, and the linear polarization fluorescence sequentially passes through the reflective objective II, the 1/2 wave plate, the Glan prism, the high-pass filter and the focusing mirror I and then is subjected to spectral measurement in the spectral acquisition system.
The sample to be measured is fixed on a translation table with XYZ direction movement freedom.
The 1/2 wave plate is provided on a motorized mirror holder having rotational freedom about the direction of light beam propagation.
The utility model has the following beneficial effects and advantages:
1. the utility model discloses be equipped with portable beam splitting piece and portable speculum, portable beam splitting piece removes to between polaroid and the speculum two, and when portable speculum removed between reflective objective two and the 1/2 wave plates, white light source and micro-imaging system were switched into the light path, and the system can carry out micro-imaging and select area to the sample of being surveyed and focus, makes and can carry out the normal position to the sample of being surveyed and measure in the repeated experimentation many times.
2. The utility model discloses be equipped with reflective objective one and reflective objective two, the novelty has removed dichroic mirror in the similar report, the collection direction that makes laser source's incident direction and surveyed sample linear polarization fluorescence beam is located respectively by survey sample both sides, the fluorescence spectrum that makes spectrum collection system gather is unanimous with the fluorescence spectrum of being surveyed sample radiation, when having eliminated laser source incident direction and being surveyed sample linear polarization fluorescence beam and collecting the direction unanimity, adopt dichroic mirror to carry out the linear polarization fluorescence polarization composition of the final collection that the beam split caused and the inconsistent problem of the linear polarization fluorescence polarization composition of sample radiation.
3. The utility model discloses the sample to be measured is installed in linear motor driven three-dimensional translation bench, in the measurement process, three-dimensional translation bench is in linear motor drive removes down, makes the sample to be measured change in the coincidence position of laser light source light beam, and the system gathers the linear polarization fluorescence of the different position radiation of sample to be measured and will linear polarization fluorescence is corresponding in the position that the sample to be measured receives excitation to the realization is measured the formation of image of the sample linear polarization fluorescence spectrum to be measured.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the focusing mode of the selected area of the present invention;
FIG. 3 is a schematic diagram of the spectral imaging measurement method of the present invention;
the system comprises a laser light source 1, a white light source 2, a first reflector 3, a polarizer 4, a movable beam splitting plate 5, a second reflector 6, a first reflection objective 7, a sample to be measured 8, a second reflection objective 9, a movable reflector 10, a 1/2 wave plate 11, a Glan prism 12, a high-pass filter 13, a first focusing mirror 14, a first light transmitting optical fiber 15, a light transmitting optical fiber 16, a spectrometer 17, a first data transmission line 18, a first computer 19, a third reflector 20, a second focusing mirror 21, a CCD camera 22, a second data transmission line 23 and a second computer 24.
Wherein 801 is an image of a tested sample 8 in a microscopic imaging system, and 101 is a sampling matrix of a laser light source 1 or a laser light source 2 focusing coincident light spots on the tested sample 8 during imaging measurement.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
The linear polarization fluorescence spectrum imaging measurement system comprises a laser light source, a white light source, a first reflecting mirror, a movable beam splitting plate, a second reflecting mirror, a first reflecting objective, a sample to be measured, a second reflecting objective, a movable reflecting mirror, 1/2 wave plates, a Glan prism, a high-pass filter plate, a first focusing mirror, a spectrum acquisition system and a micro-imaging system, wherein the second reflecting mirror, the first reflecting objective, the sample to be measured, the second reflecting objective, the movable reflecting mirror, a 1/2 wave plate, the Glan prism, the high-pass filter plate, the first focusing mirror and the spectrum acquisition system are arranged in a line, when the sample to be measured is subjected to micro-imaging, the movable beam splitting plate is moved between the second polarizing plate and the second reflecting mirror, the movable reflecting mirror is moved between the second reflecting objective and the 1/2 wave plates, white light emitted by the white light source passes through the movable beam splitting plate, the second reflecting mirror and the first reflecting objective and illuminates the sample to be measured, the measured sample is imaged in a microscopic imaging system after passing through a second reflective objective lens and a movable reflector, when the measured sample is subjected to linear polarization fluorescence spectrum collection, the movable beam splitting sheet and the movable reflector are moved out of the system, a laser beam emitted by a laser source passes through a polarizing sheet to become linear polarization excitation light, the linear polarization excitation light is focused on the measured sample through the first reflective objective lens after being reflected by the first reflector lens and the second reflector lens, the measured sample is excited to be irradiated with linear polarization fluorescence, the linear polarization fluorescence sequentially passes through the second reflective objective lens, an 1/2 wave plate, a Glan prism, a high-pass filter and a first focusing lens and then enters a spectrum collection system, and the spectrum collection system collects and obtains the fluorescence spectrum of the measured sample.
The first reflective objective lens can be replaced by a focusing system.
The movable beam splitting piece and the movable reflector are respectively provided with a moving-in system and a moving-out system, and the two states are realized through the electric overturning platform.
The polarizer and the Glan prism are respectively mounted on a frame having a rotational degree of freedom in a beam propagation direction.
The 1/2 wave plate is mounted on a motorized frame with rotational freedom in the direction of beam propagation, and rotation of the motorized frame causes rotation of the 1/2 wave plate.
The sample to be measured is mounted on a translation stage having X, Y, Z degrees of freedom of movement, which is driven by a linear motor.
The microscopic imaging system is composed of a third reflecting mirror, a second focusing mirror, an imaging camera, a second data transmission line and a second computer.
The spectrum acquisition system consists of a light transmission optical fiber, a spectrometer, a first data transmission line and a first computer, and optical fiber ends are arranged at the incident section and the emergent end of the light transmission optical fiber.
The sample to be detected can be a spin-coated solid sample, can be a diamond anvil cell high-pressure module, and can be a low-temperature sample refrigerated by liquid nitrogen or liquid helium.
As shown in fig. 1, the utility model discloses a 1, laser source, 2, white light source, 3, speculum one, 4, polaroid, 5, portable beam splitter, 6, speculum two, 7, reflective objective one, 8, the sample under test, 9, reflective objective two, 10, movable speculum, 11, 1/2 wave plate, 12, glan prism, 13, high pass filter, spectral acquisition system and micro-imaging system, wherein speculum two 6, reflective objective one 7, the sample under test 8, reflective objective two 9, movable speculum 10, 1/2 wave plate 11, glan prism 12, high pass filter 13, focusing mirror one 14 and spectral acquisition system become a linear arrangement, when carrying out micro-imaging to the sample under test 8, portable beam splitter 5 moves to between polarization 4 piece and speculum two 6, the white light that white light source 2 outgoing passes through portable beam splitter 5 in proper order, 4 polaroids, 5, The second reflecting mirror 6 and the first reflecting mirror 7 illuminate a measured sample 8, the measured sample 8 is imaged in a microscopic imaging system after passing through the second reflecting objective 9 and the movable beam splitter 10, when linear polarization fluorescence spectrum collection is carried out on the measured sample 8, the movable beam splitter 5 and the movable reflecting mirror 10 are moved out of the system, a laser beam emitted by the laser source 1 passes through the first reflecting mirror 3, the polarizing plate 4, the second reflecting mirror 6 and the first reflecting objective 7 and is focused and incident to the measured sample 8, a stimulated radiation polarized fluorescent beam of the measured sample 8 passes through the second reflecting objective 9, the 1/2 wave plate 11, the Glan prism 12, the high-pass filter 13 and the first focusing mirror 14 in sequence and then is incident to the spectrum collection system.
As shown in fig. 2, during microscopic imaging, a measured sample 8 is imaged in a microscopic imaging system, 801 is that the measured sample 8 is imaged in the microscopic imaging system, laser emitted by a laser source 1 sequentially passes through a polarizing plate 4, a movable beam splitting plate 5, a second reflecting mirror 6 and a reflective objective lens 7 and is focused on the measured sample 8 to generate a focused coincident light spot, the focused coincident light spot can also be imaged in the microscopic imaging system, 101 is that the laser source 1 is focused on the measured sample 8 to be imaged in the microscopic imaging system, by adjusting a position of the measured sample 8 perpendicular to an incident direction of the laser, a coincident position of the focused coincident light spot and a sample area of the measured sample 8 can be changed, a coincident position of the measured sample 8 and the laser is changed, so that the measured sample 8 and the laser coincide position in multiple measurements are the same, thus realizing in-situ measurement in the process of repeated measurement for many times.
As shown in fig. 3, during the linear polarization fluorescence spectrum imaging measurement, a measured sample 8 is imaged in a microscopic imaging system, a laser source 801 is imaged by the measured sample 8 in the microscopic imaging system, laser emitted from the laser source 1 sequentially passes through a polarizer 4, a movable beam splitter 5, a second reflector 6 and a reflective objective lens 7, and is focused on the measured sample 8 to generate a focused coincident light spot, which can also be imaged in the microscopic imaging system, each element 101 is a focused coincident light spot of the laser source 1 on the measured sample 8 during single acquisition in the linear polarization fluorescence spectrum imaging measurement process, and is imaged in the microscopic imaging system, the measured sample 8 is fixed on a translation stage having XYZ directional movement freedom, the translation stage movement freedom is driven by a linear motor, the linear motor can control the movement step length of the translation stage, and moving the tested sample 8 fixed on the translation stage by a fixed step length in the direction vertical to the exciting light, wherein the node of the fixed step length movement forms a sampling matrix of the linear polarization fluorescence spectrum imaging measurement, namely, the linear polarization fluorescence spectrum measurement is performed at each node.
The utility model discloses a theory of operation does:
as shown in fig. 1, the utility model discloses be equipped with portable beam splitting piece 5 and portable speculum 10, portable beam splitting piece 5 removes between polaroid 4 and speculum two 6, and portable speculum 10 removes between two and 1/2 wave plates of reflective objective 9, and white light source 2 is switched into the light path with the micro-imaging system, when carrying out the linear polarization fluorescence spectral measurement to surveyed sample 8, portable beam splitting piece 5 is shifted out from the system with portable speculum 10.
As shown in fig. 1, in the microscopic imaging process, the movable beam splitting plate 5 moves between the polarizer 4 and the second mirror 6, the movable mirror 10 moves between the second reflective objective 9 and the 1/2 wave plate 11, the white light emitted from the white light source 2 sequentially passes through the movable beam splitting plate 5, the second mirror 6 and the reflective objective 7 to illuminate the sample 8 to be measured, the sample 8 to be measured passes through the second reflective objective 9 and the movable mirror 10 to be imaged in the microscopic imaging system, the microscopic imaging system is composed of the second focusing mirror 21, the CCD camera 22, the second data transmission device 23 and the second computer 24, the sample 8 to be measured is placed on a translation stage with X, Y, Z three degrees of freedom of movement, in the microscopic imaging process, the position of the sample 8 to be measured is adjusted by adjusting the translation adjusting lever of the translation stage in the direction X, Y, Z, and the sample 8 to be measured can be a clear and complete image on the CCD camera 22, the second computer 24 receives the imaging signal transmitted by the second data transmission line 23 to display the clear and complete image.
As shown in fig. 2, in the selected area focusing process, while a measured sample 8 is imaged in a microscopic imaging system, laser emitted by a laser source 1 sequentially passes through a first reflecting mirror 3, a polarizing plate 4, a movable beam splitting plate 5, a second reflecting mirror 6 and a first reflective objective lens 7 and then is focused on a sample area of the measured sample 8, the laser forms a focused coincident spot at a focused coincident position on the measured sample, the focused coincident spot sequentially passes through a second reflective objective lens 9 and a movable reflecting mirror and is imaged in the microscopic imaging system 801, the laser source 1 forms a focused coincident spot on the measured sample 8 in the microscopic imaging system 101, the measured sample 8 and the laser are changed in coincident position by adjusting a position of the measured sample 8 perpendicular to an incident direction of the laser in a single measurement process, therefore, the excited position of the detected sample 8 is changed, the coincident position is the position of the detected sample 8 excited by the laser, and in the repeated measurement process, the coincident position of the detected sample 8 and the laser is changed by adjusting the position of the detected sample 8 vertical to the incident direction of the laser, so that the coincident position of the detected sample 8 and the laser in the repeated measurement process is the same, and the in-situ measurement in the repeated measurement process is realized.
As shown in fig. 1, in the process of measuring the linearly polarized fluorescence spectrum, the movable beam splitter 5 and the movable reflector 10 are moved out of the system, the laser emitted from the laser source 1 is reflected by the first reflector 3 and then enters the polarizer 4, the polarizer 4 is mounted on the mirror holder with the rotational degree of freedom around the propagation direction of the laser, the polarization direction of the polarizer 4 is changed by adjusting the mirror holder, the laser passes through the polarizer 4 and then becomes a pure linearly polarized laser, the pure linearly polarized laser sequentially passes through the second reflector 6 and the reflective objective 7 and then is focused on the sample 8 to be measured, the sample 8 to be measured is excited by the pure linearly polarized laser to emit linearly polarized fluorescence, the linearly polarized fluorescence is composed of linearly polarized beams with mutually perpendicular polarization directions, the linearly polarized fluorescence is collimated by the reflective objective 9 into a parallel beam and then enters the 1/2 wave plate 11, 1/2 wave plate 11 is installed on the electric rotating frame with freedom of rotation around the light beam transmission direction, the electric rotating frame is set to rotate at 0 degree position and 45 degree position, the 0 degree position and 45 degree position cooperate with the Glan prism 12 to respectively extract two polarized light beams of the linear polarized fluorescent light beam with mutually perpendicular polarization directions, so that the two polarized light beams respectively enter the spectrum collection system with the 0 degree position and 45 degree position of the electric rotating frame, the linear polarized fluorescent light beam extracted by the Glan prism is focused on the light transmission fiber input terminal 15 of the light transmission fiber 16 through the filtering of the high pass filter 13 and the focusing lens 14, the light transmission fiber input terminal 15 is installed on the frame with freedom of movement vertical to the light path transmission direction, the position of the light transmission fiber input terminal 15 is changed by adjusting the frame with freedom of movement, the linear polarization fluorescent light beam has the maximum coupling efficiency on the light transmission optical fiber input end 15, the linear polarization fluorescent light beam enters the light transmission optical fiber input end 15 and is transmitted to the spectrometer 17 through the light transmission optical fiber 16, the spectrometer 17 collects and obtains the spectrum information of the linear polarization fluorescent light beam and converts the spectrum information into an electric signal, the telecommunication signal is transmitted to the first computer 19 through the first data transmission 18, and the first computer displays and stores the electric signal converted from the linear polarization fluorescent light spectrum.
In the process of imaging the linear polarized fluorescence spectrum, a tested sample 8 is fixed on a three-dimensional translation table with the freedom of movement in the XYZ direction, the freedom of movement of the translation table is driven by a linear motor, before the imaging measurement process, microscopic imaging of the tested sample 8 is carried out, the initial position of the imaging process, namely the overlapping position of a sample area of the tested sample 8 and the focus of the exciting light, is determined, a first group of linear polarized fluorescence spectra are acquired after the initial position is determined, after the initial position linear polarized fluorescence spectra are acquired, the three-dimensional translation position is changed through the linear motor, then the overlapping position of the tested sample 8 and the focus of the exciting light is changed, the distance between the changed position of the tested sample and the initial position is a moving step length, the linear polarized fluorescence spectra after the position change are continuously acquired, and the operation of changing the position of the step length distance and acquiring the linear polarized fluorescence spectra is repeated, the acquisition positions are enabled to cover the sample area of the tested sample 8 in a matrix form, and the linear polarization fluorescence imaging spectrogram of the tested sample 8 can be synthesized through software after the linear polarization fluorescence spectra of all matrix element positions are acquired.
The utility model discloses accomplish the microscopic formation of image and the selected area back of focusing of being surveyed sample 8, carry out the linear polarization fluorescence spectral measurement to being surveyed sample 8 at different positions and realize the linear polarization fluorescence spectral imaging measurement.
In this embodiment, the first reflective objective lens 7 and the second reflective objective lens 9 are preferably 15-fold objective lenses, and the first focusing lens 14 is preferably 100mm in focal length.
Claims (10)
1. A microscopic linear polarization fluorescence spectral imaging measurement system, comprising: the device comprises a second reflecting mirror (6), a first reflecting objective lens (7), a second reflecting objective lens (9), a movable reflecting mirror (10), an 1/2 wave plate (11), a Glan prism (12), a high-pass filter plate (13) and a first focusing mirror (14) which are sequentially arranged in a line, and further comprises a light source generating system, a microscopic imaging system and a spectrum collecting system;
the light source generating system is arranged on an incident light path of the second reflector (6); the microscopic imaging system is arranged on a reflection light path of the movable reflector (10); the spectrum acquisition system is arranged on a transmission light path of the first focusing mirror (14); and a space for placing a sample (8) to be measured is arranged between the first reflective objective lens (7) and the second reflective objective lens (9).
2. The microscopic linear polarized fluorescence spectral imaging measurement system of claim 1, wherein the light source generation system comprises: the device comprises a second reflector (6), a movable beam splitting sheet (5), a polarizing sheet (4) and a first reflector (3), wherein the movable beam splitting sheet, the polarizing sheet and the first reflector (3) are sequentially arranged in a line on an incident light path of the second reflector, and the device also comprises a laser light source (1) arranged on the incident light path of the first reflector (3) and a white light source (2) arranged on the incident light path of the movable beam splitting sheet (5).
3. A microscopic linear polarized fluorescence spectral imaging measurement system according to claim 2, characterized in that the polarizer (4) is mounted on the frame with rotational freedom around the laser propagation direction.
4. The microscopic linear polarized fluorescence spectral imaging measurement system of claim 1, wherein the microscopic imaging system comprises: the device comprises a third reflecting mirror (20), a second focusing mirror (21), a CCD camera (22), a second data transmission line (23) and a second computer (24), wherein the CCD camera (22) is connected with the second computer (24) through the second data transmission line (23), and the third reflecting mirror (20), the second focusing mirror (21) and the CCD camera (22) are sequentially arranged on a reflecting light path of the movable reflecting mirror (10).
5. The microscopic linear polarized fluorescence spectral imaging measurement system of claim 1, wherein the microscopic imaging system comprises: the spectrometer comprises a light transmitting optical fiber input end (15), a light transmitting optical fiber (16), a spectrometer (17), a first data transmission line (18) and a first computer (19) which are connected in sequence, wherein the light transmitting optical fiber input end (15) is arranged on a transmission light path of a focusing mirror I (14).
6. A linearly polarized fluorescence spectroscopic imaging measurement system according to claim 5 wherein the light transmitting fiber input tip (15) is mounted on a frame with freedom of movement perpendicular to the direction of propagation of the light path.
7. The microscopic linear polarization fluorescence spectrum imaging measurement system according to claim 1 or 2, wherein when the measured sample is subjected to microscopic imaging, the movable beam splitter (5) moves to a position between the polarizer (4) and the second reflector (6), the movable reflector (10) is positioned between the second reflector (9) and the 1/2 wave plate (11), and the white light emitted by the white light source (2) sequentially passes through the movable beam splitter (5), the second reflector (6) and the first reflector (7) to illuminate the measured sample (8), so that the measured sample (8) sequentially passes through the second reflector (9) and the movable reflector (10) to be imaged in the microscopic imaging system.
8. The microscopic linear polarization fluorescence spectrum imaging measurement system according to claim 1 or 2, characterized in that when performing spectrum measurement on a sample to be measured, the movable beam splitter (5) and the movable reflector (10) are respectively moved out of a line arrangement, a natural excitation beam emitted by the laser source (1) sequentially passes through the first reflector (3), the polarizer (4), the second row reflector (6), the first reflective objective (7) and irradiates on the sample to be measured (8), so that the sample to be measured (8) is excited to radiate linear polarization fluorescence, and then sequentially passes through the second reflective objective (9), the 1/2 wave plate (11), the glan prism (12), the high pass filter (13) and the first focusing mirror (14) to perform spectrum measurement in the spectrum collection system.
9. A microscopic linear polarization fluorescence spectrum imaging measurement system according to claim 1, wherein the sample (8) to be measured is fixed on a translation stage having freedom of movement in XYZ directions.
10. The imaging measurement system of microscopic linear polarized fluorescence spectrum according to claim 1, characterized in that the 1/2 wave plate (11) is arranged on a motorized frame with rotational freedom around the light beam propagation direction.
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