CN217103882U

CN217103882U - Sample adding device for fluorescent quantitative PCR instrument

Info

Publication number: CN217103882U
Application number: CN202220210975.7U
Authority: CN
Inventors: 韦学雷; 李炳晓; 陈跃鹏; 袁世轩
Original assignee: Henan Wilpigo Biotechnology Development Co ltd
Current assignee: Henan Wilpigo Biotechnology Development Co ltd
Priority date: 2022-01-25
Filing date: 2022-01-25
Publication date: 2022-08-02
Anticipated expiration: 2032-01-25

Abstract

The utility model discloses a sample adding device for a fluorescence quantitative PCR instrument, which relates to the technical field of fluorescence quantitative PCR instruments and solves the technical problems of manual operation and low automation degree of the existing fluorescence quantitative PCR sample adding, comprising a liquid transferring gun, wherein the top of the liquid transferring gun is provided with a movable block, vertical plates are symmetrically arranged on the two sides of the liquid transferring gun vertically, a back-and-forth driving mechanism for driving the movable block to move back and forth is arranged between the vertical plates, a sample supplying mechanism is communicated on the liquid transferring gun, a PCR tube placing rack is arranged below the liquid transferring gun, and a lifting driving mechanism is arranged at the bottom of the PCR tube placing rack; the height of the PCR tube placing frame is adjusted, and the liquid transferring gun moves back and forth to automatically and quickly add samples into the PCR tube a, so that the sample adding is quick and the working efficiency is high.

Description

Sample adding device for fluorescent quantitative PCR instrument

Technical Field

The utility model relates to a fluorescence quantitative PCR appearance technical field, more specifically relate to fluorescence quantitative PCR appearance application of sample equipment technical field.

Background

The real-time fluorescent quantitative PCR technology was introduced by Applied Biosystems in 1996, and has been widely used at present because the technology not only realizes qualitative to quantitative leap of PCR, but also has the characteristics of stronger specificity, effective solution of PCR pollution problem, high automation degree and the like compared with the conventional PCR. Real-time quantitative PCR technique: and detecting the change of the amount of each cycle of amplified product in the PCR amplification reaction in real time by utilizing the change of the fluorescent signal, and quantitatively analyzing the initial template through the analysis of a Ct value and a standard curve.

When the fluorescent quantitative PCR is added, all the adding operations are aimed at making the reaction system uniform as much as possible, except for the sample, other reagents are premixed, the premixed reagents are subpackaged into each PCR tube, and finally the sample is added, the pipettor is accurate as much as possible, because the operation at the step can be said to be millicentimetre and thousands of miles. Application No.: 202022820839.3 discloses a full-automatic application of sample mechanism of fluorescence quantitative PCR appearance, through having set up flow control device at application of sample pipe surface lower extreme, through the slow rotation swivel becket, the swivel becket drives the adjustment disk and rotates, make the regulation mouth in the adjustment disk coincide with the opening in the fixed disk gradually in order to adjust the flow, can be to the different velocity of flow control of difference during the application of sample, reach the effect of supplementary regulation application of sample flow, through having set up the filter in application of sample pipe surface lower extreme eighth department, through inserting the filter screen in the notch, make the filter screen filter the sample, reach the effect of supplementary filtration sample, the device convenient to use easy operation, the effectual work efficiency that has improved.

The defects of the above patent are that the sample can only be manually added to the PCR tube, the automation degree is low, and the working efficiency is low.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a: in order to solve above-mentioned fluorescence ration PCR application of sample manual operation, the technical problem that degree of automation is low, the utility model provides a fluorescence ration is application of sample device for PCR appearance.

The utility model discloses a realize above-mentioned purpose and specifically adopt following technical scheme: the utility model provides a fluorescence ration application of sample device for PCR appearance, includes the pipette, the pipette top is provided with the movable block, the vertical symmetry in pipette both sides is provided with the riser, be provided with the actuating mechanism that makes a round trip that is used for driving the movable block round trip movement between the riser, the intercommunication has confession sample mechanism on the pipette, the below of pipette is provided with PCR pipe rack, the bottom of PCR pipe rack is provided with lift actuating mechanism.

The working and using process is as follows: when the sample feeding mechanism is used, a PCR tube a is placed on the PCR tube placing frame, the PCR tube a can be single tube or connected tubes, the lifting driving mechanism is started to move the PCR tube placing frame to a proper height, the cover of the PCR tube a is opened, the sample feeding mechanism is simultaneously opened, the sample flows into the pipetting gun, the reciprocating driving mechanism drives the moving block to stably drive the pipetting gun to horizontally reciprocate, a proper amount of sample is added into each PCR tube a, after the sample is added into the PCR tube a, the PCR tube placing frame drives all the PCR tubes a to move downwards, the moving block drives the pipetting gun to move to the upper side of the next PCR tube a, the PCR tube placing frame drives all the PCR tubes a to move upwards, then the cover on the PCR tubes a is closed, after the sample is added, the lifting driving mechanism drives the PCR tube placing frame to move downwards, the PCR tubes a after the sample is added are taken away, the PCR tubes a needing new sample addition are placed again, repeating the above steps and adding sample again. Through setting up of pipetting gun, movable block, round trip drive mechanism, PCR pipe rack and lift actuating mechanism, adjust the height that PCR pipe rack extremely, pipetting gun round trip movement has simultaneously realized adding the appearance to PCR pipe an automatically and fast, and the application of sample is fast, and work efficiency is high, has solved above-mentioned fluorescence ration PCR application of sample manual operation, the technical problem that degree of automation is low.

Furthermore, a vertical pipe is arranged in the pipette, the vertical pipe extends to the outer side of the bottom of the pipette, a flow meter is arranged on the vertical pipe on the outer side of the bottom of the pipette, a dropper is communicated with the bottom of the vertical pipe, a first liquid pump is connected to the top of the vertical pipe in the pipette, and the sample supply mechanism is communicated with the vertical pipe in the pipette. The sample in the sample supply mechanism enters the vertical tube, falls under the action of the first liquid pump, and the accurate amount of the sample is metered by the flow meter and enters the PCR tube a from the dropper.

Furthermore, the sample supplying mechanism comprises a sample box located on the vertical plate, a first sample conveying pipe communicated with the sample box, a hose communicated with the first sample conveying pipe, a second sample conveying pipe communicated with the hose, and a second liquid pump located on the first sample conveying pipe, wherein the sample box is located on the upper portion of the outer side of the vertical plate. The sample box is internally provided with a sample after being processed and filtered, under the action of the second liquid pump, the sample sequentially passes through the first sample conveying pipe, the hose and the second sample conveying pipe and then enters the vertical pipe, and the hose is an extensible or shortened pipe which is convenient to move back and forth with the liquid-transfering gun at present.

Furthermore, the back-and-forth driving mechanism comprises a screw rod positioned between the vertical plates, limiting rods positioned on two sides of the screw rod and positioned between the vertical plates and a motor positioned at one end of the screw rod; the middle of the moving block is arranged in a first through hole matched with the screw rod, two sides of the moving block are respectively provided with a second through hole matched with the limiting rod, and threads matched with the screw rod are arranged in the first through hole. The first motor drives the lead screw to rotate, threads matched with the lead screw are arranged in the first through hole in the moving block, the moving block is limited by the two limiting rods, the moving block can only move back and forth along the lead screw, and the first motor drives the lead screw to rotate forwards or backwards in a conventional mode in the field.

Furthermore, the lifting driving mechanism comprises at least two jackets below the PCR tube placing rack, straight racks positioned in each jacket, a driving gear meshed with each straight rack and a motor driving the driving gear, and the top of each straight rack is fixed at the bottom of the PCR tube placing rack. The jacket is fixed with a second motor, the second motor drives a driving gear to rotate, the driving gear is meshed with a spur rack, so that the driving gear drives the spur rack to move up and down, and the spur rack moves up and down to drive the PCR tube placing rack to move up and down as the upper part of the spur rack is fixed with the PCR tube placing rack.

Furthermore, the bottom of the PCR tube placing rack is also provided with at least one telescopic rod. The upper portion of telescopic link is fixed in the bottom of PCR pipe rack, and the telescopic link reciprocates and stretches out and draws back from top to bottom along with the spur rack, and the telescopic link plays the guide effect, makes whole PCR pipe rack steadily reciprocate.

The utility model has the advantages as follows:

1. the height of the PCR tube placing frame is adjusted by arranging the pipette, the moving block, the back-and-forth driving mechanism, the PCR tube placing frame and the lifting driving mechanism, and meanwhile, the pipette moves back and forth to automatically and rapidly add samples into the PCR tube a, so that the sample adding is rapid and the working efficiency is high;

2. the sample in the sample mechanism enters the vertical pipe, falls under the action of the first liquid pump, and the sample with accurate quantity is metered by the flowmeter and enters the PCR tube a from the dropper;

3. the sample box is internally provided with a processed and filtered sample, under the action of a second liquid pump, the sample sequentially passes through a first sample conveying pipe, a hose and a second sample conveying pipe and then enters a vertical pipe, and the hose is an extensible or shortened pipe which is convenient to move back and forth with a liquid-transfering gun at present;

4. the first motor drives the lead screw to rotate, threads matched with the lead screw are arranged in a first through hole in the moving block, and meanwhile, the moving block is limited by two limiting rods and can only move back and forth along the lead screw;

5. the jacket is fixed with a second motor, the second motor drives a driving gear to rotate, and the driving gear is meshed with the straight rack, so that the driving gear drives the straight rack to move up and down;

6. the upper portion of telescopic link is fixed in the bottom of PCR pipe rack, and the telescopic link reciprocates and stretches out and draws back from top to bottom along with the spur rack, and the telescopic link plays the guide effect, makes whole PCR pipe rack steadily reciprocate.

Drawings

FIG. 1 is a schematic structural view of a sample adding device for a fluorescence quantitative PCR instrument of the present invention;

FIG. 2 is a schematic structural view of the back and forth driving mechanism of the present invention;

fig. 3 is a schematic structural diagram of the driving gear and the spur rack of the present invention.

Reference numerals: 1-vertical plate, 2-second sample conveying pipe, 3-flexible pipe, 4-first sample conveying pipe, 5-second liquid pump, 6-sample box, 7-screw rod, 8-moving block, 9-first liquid pump, 10-first motor, 11-liquid-transferring gun, 12-vertical pipe, 13-flow meter, 14-dropper, 15-PCR pipe a, 16-driving gear, 17-telescopic rod, 18-jacket, 19-straight rack, 20-PCR pipe rack, 21-limiting rod, and 22-second motor.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

Example 1

As shown in fig. 1-2, this embodiment provides a sample adding device for a fluorescence quantitative PCR instrument, including a pipette 11, a moving block 8 is disposed at the top of the pipette 11, vertical plates 1 are disposed on two sides of the pipette 11 in vertical symmetry, a back-and-forth driving mechanism for driving the moving block 8 to move back and forth is disposed between the vertical plates 1, a sample supply mechanism is communicated with the pipette 11, a PCR tube placing rack 20 is disposed below the pipette 11, and a lifting driving mechanism is disposed at the bottom of the PCR tube placing rack 20.

The working and using process is as follows: when the filtered sample is arranged in the sample supplying mechanism, a PCR tube a15 is placed on a PCR tube placing rack 20, the PCR tubes a15 can be single tubes or connected tubes, a lifting driving mechanism is started to move the PCR tube placing rack 20 to a proper height, the cover of the PCR tube a15 is opened, the sample supplying mechanism is opened at the same time, the sample flows into a pipetting gun 11, the reciprocating driving mechanism drives a moving block 8 to stably drive the pipetting gun 11 to horizontally reciprocate simultaneously, a proper amount of sample is added into each PCR tube a15, after the sample is added into the PCR tube a15, the PCR tube placing rack 20 drives all PCR tubes a15 to move downwards, the moving block 8 drives the pipetting gun 11 to move to the position above the next PCR tube a15, the PCR tube placing rack 20 drives all PCR tubes a15 to move upwards for sample adding, then the cover on the PCR tube a15 is closed, after the sample adding is completed, the lifting driving mechanism drives the PCR tube placing rack 20 to move downwards, the PCR tubes a15 after the sample adding is taken away, and the PCR tubes a15 which need to be new PCR tubes are placed again, repeating the above steps and adding sample again. Through the setting of pipetting gun 11, movable block 8, round trip drive mechanism, PCR pipe rack 20 and lift actuating mechanism, adjust the height that PCR pipe rack 20 extremely, pipetting gun 11 round trip movement has simultaneously realized adding the appearance to PCR pipe a15 automatically and fast, and the application of sample is fast, and work efficiency is high, has solved above-mentioned fluorescence ration PCR application of sample manual operation, the technical problem that degree of automation is low.

Example 2

As shown in fig. 1, a vertical tube 12 is arranged in the pipette 11, the vertical tube 12 extends to the outer side of the bottom of the pipette 11, a flow meter 13 is arranged on the vertical tube 12 on the outer side of the bottom of the pipette 11, a dropper 14 is communicated with the bottom of the vertical tube 12, a first liquid pump 9 is connected to the top of the vertical tube 12 in the pipette 11, and the sample supply mechanism is communicated with the vertical tube 12 in the pipette 11; the sample supplying mechanism comprises a sample box 6 positioned on the vertical plate 1, a first sample conveying pipe 4 communicated with the sample box 6, a hose 3 communicated with the first sample conveying pipe 4, a second sample conveying pipe 2 communicated with the hose 3 and a second liquid pump 5 positioned on the first sample conveying pipe 4, wherein the sample box 6 is positioned on the upper part of the outer side of the vertical plate 1. The sample in the sample mechanism enters the vertical tube 12, falls under the action of the first liquid pump 9, and enters the PCR tube a15 from the dropper 14 after being metered by the flowmeter 13 in an accurate amount; the sample box 6 is filled with a processed and filtered sample, under the action of the second liquid pump 5, the sample sequentially passes through the first sample conveying pipe 4, the hose 3 and the second sample conveying pipe 2 and then enters the vertical pipe 12, and the hose 3 is an extensible or contractible pipe which is convenient to move back and forth with the liquid-transfering gun 11 at present.

Example 3

As shown in fig. 1-2, the back-and-forth driving mechanism includes a screw rod 7 located between the vertical plates 1, a limiting rod 21 located at both sides of the screw rod 7 and located between the vertical plates 1, and a motor located at one end of the screw rod 7; the middle of the moving block 8 is provided with a first through hole matched with the screw rod 7, two sides of the moving block 8 are respectively provided with a second through hole matched with the limiting rod 21, and threads matched with the screw rod 7 are arranged in the first through hole. The first motor 10 drives the lead screw 7 to rotate, threads matched with the lead screw 7 are arranged in a first through hole in the moving block 8, meanwhile, the moving block 8 is limited through two limiting rods 21, the moving block 8 can only move back and forth along the lead screw 7, and the first motor 10 drives the lead screw 7 to rotate forwards or backwards is a conventional mode in the field.

Example 4

As shown in fig. 3, the lifting driving mechanism comprises at least two jackets 18 located below the PCR tube placing rack 20, a spur rack 19 located in each jacket 18, a driving gear 16 engaged with each spur rack 19, and a motor driving the driving gear 16, wherein the top of each spur rack 19 is fixed at the bottom of the PCR tube placing rack 20; the bottom of the PCR tube placing frame 20 is also provided with at least one telescopic rod 17. The jacket 18 is fixed with a second motor 22, the second motor 22 drives the driving gear 16 to rotate, the driving gear 16 is meshed with the straight rack 19, so that the driving gear 16 drives the straight rack 19 to move up and down, and the straight rack 19 moves up and down to drive the PCR tube placing rack 20 to move up and down as the upper part of the straight rack 19 is fixed with the PCR tube placing rack 20; the upper portion of the telescopic rod 17 is fixed at the bottom of the PCR tube placing frame 20, the telescopic rod 17 stretches up and down along with the up-and-down movement of the straight rack 19, and the telescopic rod 17 plays a guiding role, so that the whole PCR tube placing frame 20 stably moves up and down.

Claims

1. The utility model provides a fluorescence ration application of sample device for PCR appearance, includes pipette (11), its characterized in that, pipette (11) top is provided with movable block (8), pipette (11) both sides vertical symmetry is provided with riser (1), be provided with the round trip driving mechanism who is used for driving movable block (8) round trip movement between riser (1), the intercommunication has confession sample mechanism on pipette (11), the below of pipette (11) is provided with PCR pipe rack (20), the bottom of PCR pipe rack (20) is provided with lift actuating mechanism.

2. The sample adding device for the fluorescence quantitative PCR instrument according to claim 1, wherein a vertical tube (12) is disposed in the pipette gun (11), the vertical tube (12) extends to the outside of the bottom of the pipette gun (11), a flow meter (13) is disposed on the vertical tube (12) outside of the bottom of the pipette gun (11), a dropper (14) is connected to the bottom of the vertical tube (12), a first liquid pump (9) is connected to the top of the vertical tube (12) in the pipette gun (11), and the sample supplying mechanism is communicated with the vertical tube (12) in the pipette gun (11).

3. The sample adding device for the fluorescence quantitative PCR instrument according to claim 1 or 2, wherein the sample supplying mechanism comprises a sample box (6) located on the vertical plate (1), a first sample conveying pipe (4) communicated with the sample box (6), a hose (3) communicated with the first sample conveying pipe (4), a second sample conveying pipe (2) communicated with the hose (3) and a second liquid pump (5) located on the first sample conveying pipe (4), and the sample box (6) is located on the upper portion of the outer side of the vertical plate (1).

4. The sample adding device for the fluorescence quantitative PCR instrument according to claim 1, wherein the back-and-forth driving mechanism comprises a screw rod (7) positioned between the vertical plates (1), a limiting rod (21) positioned at two sides of the screw rod (7) and positioned between the vertical plates (1), and a first motor (10) positioned at one end of the screw rod (7).

5. The sample adding device for the fluorescence quantitative PCR instrument as claimed in claim 4, wherein the moving block (8) is provided at the middle thereof with a first through hole matched with the screw rod (7), the moving block (8) is provided at both sides thereof with second through holes matched with the limiting rods (21), and the first through hole is provided with threads matched with the screw rod (7).

6. The sample adding device for the fluorescence quantitative PCR instrument according to claim 1, wherein the lifting driving mechanism comprises at least two jackets (18) under the PCR tube placing rack (20), a spur rack (19) in each jacket (18), a driving gear (16) engaged with each spur rack (19) and a second motor (22) driving the driving gear (16), and the top of each spur rack (19) is fixed at the bottom of the PCR tube placing rack (20).

7. The sample adding device for the fluorescence quantitative PCR instrument as claimed in claim 6, wherein the bottom of the PCR tube holder (20) is further provided with at least one telescopic rod (17).