CN217093553U

CN217093553U - Micro-fluidic detection chip

Info

Publication number: CN217093553U
Application number: CN202220325165.6U
Authority: CN
Inventors: 赵逸祥; 余波; 陈彦豪; 章诗校; 吴和晋; 阮泽军
Original assignee: Zhejiang Pushkang Biotechnology Co ltd
Current assignee: Zhejiang Pushkang Biotechnology Co ltd
Priority date: 2022-02-17
Filing date: 2022-02-17
Publication date: 2022-08-02
Anticipated expiration: 2032-02-17

Abstract

The invention provides a microfluidic detection chip which comprises a sheet-shaped body and a plurality of detection units arranged on the body, wherein the detection units are radially arranged on the body and comprise a sample area, a reagent area, a detection area and a waste liquid storage area. The sample area is at least used for injecting a sample; the reagent area is used for injecting a functional reagent, and comprises a reagent groove which is communicated with the sample area through a first micro-channel; the detection area is used for carrying out mixed reaction of the functional reagent and the sample, and the detection area is communicated with the reagent area through a second micro-channel; the waste liquid storage area is used for containing the reacted functional reagent and the sample, and the waste liquid storage area is communicated with the detection area through a third micro-channel. The microfluidic detection chip can realize multi-person multi-item detection, has a simple structure, and can reduce the production and manufacturing cost and the detection cost of the microfluidic detection chip.

Description

Micro-fluidic detection chip

Technical Field

The invention relates to the technical field of in-vitro microfluidic detection, in particular to a microfluidic detection chip.

Background

The micro-fluidic detection chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis on a micron-scale chip, and automatically completes the whole analysis process. Has great potential in the fields of biology, chemistry, medicine and the like.

However, the microfluidic chip technology in the prior art has some defects in practical application, such as complex structure, large sample usage amount, inaccurate detection result, single detection item and detection sample, high production cost, and the like.

Disclosure of Invention

The invention mainly aims to provide a microfluidic detection chip to solve the problems of single detection item and single detection sample of the existing microfluidic detection chip.

In order to achieve the above object, the present invention provides a microfluidic detection chip, including a sheet-shaped body and a plurality of detection units disposed on the body, wherein the plurality of detection units are radially disposed on the body, and the detection units include:

a sample region for at least sample injection;

a reagent zone for injecting a functional reagent, the reagent zone comprising a reagent reservoir communicating with the sample zone via a first microchannel;

the detection area is used for carrying out mixed reaction of a functional reagent and a sample, and the detection area is communicated with the reagent area through a second micro-channel; and

and the waste liquid storage area is used for containing the reacted functional reagent and the sample, and is communicated with the detection area through a third micro-channel.

Further, the sample area includes a sample separation unit, and the sample separation unit area includes:

the injection groove is used for injecting a sample;

the plasma/serum groove is communicated with the injection groove and is used for temporarily storing the separated plasma/serum;

the blood cell groove is positioned on one side of the plasma/serum groove, which is far away from the injection groove, and is communicated with the plasma/serum groove, and the blood cell groove is used for temporarily storing the separated blood cells; and

a holding tank in communication with the plasma/serum tank, the holding tank for quantifying sample injection volume.

Further, a connecting area is arranged on one side of the plasma/serum groove, which is far away from the injection groove, and the first micro-channel is communicated with the plasma/serum groove through the connecting area;

the sample separation unit further comprises a first connecting channel, the blood ball groove is communicated with the connecting area through the first connecting channel, and the depths of the first connecting channel and the first micro-channel are smaller than the depth of the connecting area.

Further, a first micro-flow valve is arranged on the first micro-flow channel, and the depth of the first micro-flow valve is at least 1mm deeper than that of the first micro-flow channel; and/or the presence of a gas in the gas,

and a second micro-flow valve is arranged on the third micro-flow channel, and the depth of the second micro-flow valve is at least 1mm deeper than that of the second micro-flow channel.

Further, the detection unit further comprises a buffer area, the third micro-channel is connected between the detection area and the buffer area, and the buffer area is communicated with the waste liquid storage area through a second connecting channel.

Further, the buffer area is communicated with the waste liquid storage area through at least two second connecting channels.

Further, the volume of the waste liquid storage area is larger than that of the buffer area, wherein,

the waste liquid storage area is positioned on one side of the detection area far away from the reagent area and comprises a first edge arranged close to the buffer area, the first edge comprises a first end region, a middle region and a second end region, the first end region, the middle region and the second end region are sequentially arranged along the circumferential direction of the body, and the first end region is connected with the middle region through a first recessed region, and the middle region is connected with the second end region through a second recessed region, the first and second recessed regions are recessed toward a direction away from the buffer zone, the first end region and the second end region are convex toward a direction close to the buffer area, the communication position of the second connecting channel and the waste liquid storage area is positioned in the middle area.

Further, the detection unit further comprises a stop groove, and the stop groove is arranged on one side, away from the detection area, of the waste liquid storage area.

Further, the backstop recess extends along the circumference of body, and the length of backstop recess is no less than waste liquid storage area is along ascending length in the body circumference.

Further, the third micro flow channel is provided with a convex part which is convex towards the direction of the center of the body, and the distance from the vertex of the convex part to the center of the body is not less than the distance from one end, close to the center of the body, of the detection area to the center of the body.

By applying the technical scheme of the invention, as the microfluidic detection chip is provided with the plurality of detection units, the structure of each detection unit is simple, and the sequential release of the functional reagent, the sample and the like can be realized only by controlling the rotation speed, the direction and the like of the detection instrument. Meanwhile, the microfluidic detection chip can realize multi-person multi-item detection, has a simple structure, and can reduce the production and manufacturing cost and the detection cost of the microfluidic detection chip.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:

FIG. 1 is a top view of a microfluidic detection chip according to an embodiment of the present disclosure;

FIG. 2 is a top view of a detection unit of the microfluidic detection chip disclosed in the embodiments of the present invention;

FIG. 3 is a flow chart of sample separation of the microfluidic detection chip disclosed in the embodiments of the present invention;

FIG. 4 is a flow chart of the cleaning process of the microfluidic detection chip disclosed in the embodiment of the present invention;

fig. 5 is a flow chart of channel evacuation of the microfluidic chip according to the embodiment of the present invention.

Wherein the figures include the following reference numerals:

10. a body; 20. a detection unit; 21. a sample area; 211. injecting into a groove; 212. a plasma/serum sink; 213. a blood cell groove; 214. a storage tank; 215. a connecting region; 216. a first connecting passage; 217. a first air vent; 218. a first gas-permeable groove; 22. a reagent zone; 221. a reagent tank; 222. injecting a reagent into a position; 23. a detection zone; 24. a waste liquid storage area; 241. a first edge; 2411. a first end region; 2412. a middle region; 2413. a second end region; 2414. a first recessed region; 2415. a second recessed region; 242. a second edge; 25. a first microchannel; 26. a second microchannel; 27. a third microchannel; 271. a boss portion; 28. a second micro flow valve; 29. a buffer area; 201. a second connecting channel; 202. a stopper groove; 203. a second air hole; 204. a second air-permeable groove.

Detailed Description

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as exemplary only and not as limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.

Referring to fig. 1 and fig. 2, wherein fig. 1 is a top view of a microfluidic chip according to an embodiment of the present invention, and fig. 2 is a top view of a detection unit of the microfluidic chip according to the present invention. According to the embodiment of the utility model, a micro-fluidic detection chip is provided, this micro-fluidic detection chip includes chip upper strata (not shown in the figure), intermediate level (not shown in the figure) and body 10. Wherein, the chip upper strata plays the guard action as the casing part of micro-fluidic detection chip to body 10, and the intermediate level covers on body 10, can play sealed effect to body 10, is provided with the filling hole that supplies sample or reagent to pass through on chip upper strata and the intermediate level, and during the actual equipment, can be in the same place through mode fixed connection such as bonding, joint, spiro union or welding between chip upper strata, intermediate level and the body 10.

Specifically, the body 10 of the microfluidic detection chip in this embodiment is arranged in a sheet shape, and the sheet-shaped body 10 may be a circular sheet-shaped structure, a polygonal sheet-shaped structure, or another irregular sheet-shaped structure, and the drawings in this embodiment show the case where the body 10 is arranged in a circular sheet-shaped structure. The surface of the body 10 is provided with a plurality of detecting units 20, the plurality of detecting units 20 are radially arranged on the body 10, the plurality of detecting units 20 may be two, three, four, five or more, and fig. 1 in the embodiment shows a case where there are 12 detecting units 20. It is understood that the shape of each detecting unit 20 in the present embodiment is generally a sector (the outer contour of the detecting unit 20 is generally a sector), and in this case, the plurality of detecting units 20 are radially disposed on the body 10 means that the circle center side of each detecting unit 20 is disposed near the center position of the body 10, the radial direction is consistent with the radial direction of the body 10, and the plurality of detecting units 20 are sequentially arranged along the circumferential direction of the body 10.

Further, the detecting unit 20 includes a sample area 21, a reagent area 22, a detection area 23, and a waste liquid storage area 24. Wherein the sample area 21 is at least used for injecting a sample, wherein the sample may be a blood sample, for example, but of course, in other embodiments of the present invention, the sample may also be urine, etc.; the reagent zone 22 is used for injecting a functional reagent, the reagent zone 22 includes a reagent groove 221, the reagent groove 221 is communicated with the sample zone 21 through a first micro flow channel 25; the detection area 23 is used for carrying out a mixing reaction of the functional reagent and the sample, and the detection area 23 is communicated with the reagent area 22 through a second micro-channel 26; the waste liquid storage area 24 is used for containing the reacted functional reagent and the sample, and the waste liquid storage area 24 is communicated with the detection area 23 through a third micro channel 27.

In practical use, the microfluidic chip in this embodiment is placed in a matching detection apparatus, and at this time, the sample and the functional reagent can be added to the sample area 21 and the reagent area 22 respectively by the action of the sampling needle and the reagent needle on the detection apparatus. After the sample enters the sample area 21, the detection instrument can drive the microfluidic detection chip to rotate, so that the sample can be transported from the first microchannel 25 to the reagent area 22 and then to the detection area 23 through the second microchannel 26, and similarly, the functional reagent entering the reagent area 22 can be transported to the detection area 23 through the second microchannel 26 under the action of centrifugal force in the rotating process of the microfluidic detection chip. In the process, the rotating speed of the detecting instrument can be controlled by detecting personnel according to actual detection requirements and detection items to realize sequential release of the functional reagent and the sample, after the functional reagent and the sample enter the detection area 23, mixed reaction can be carried out in the area, then the detection area 23 can be incubated, and operations such as cleaning and the like can be carried out, the cleaning solution and the like can be conveyed to the waste liquid storage area 24 through the third micro flow channel 27 under the action of centrifugal force, finally, the detection area 23 is optically detected through a light path detecting device on the detecting instrument, signals obtained by the detection are transmitted to a control unit on the detecting instrument, and corresponding detection results can be obtained through calculation of the control unit.

Compared with the existing microfluidic detection chip, the microfluidic detection chip in the embodiment is provided with the plurality of detection units 20, each detection unit 20 has a simple structure, and the sequential release of the functional reagent, the sample and the like can be realized only by controlling the rotation speed, the direction and the like of the detection instrument. Meanwhile, the microfluidic detection chip can realize multi-person multi-item detection, has a simple structure, and can reduce the production and manufacturing cost and the detection cost of the microfluidic detection chip.

The following describes each region of the microfluidic detection chip in detail with reference to the accompanying drawings.

Referring to fig. 2, the sample area 21 in the present embodiment comprises a sample separation unit which can be used for separating and injecting a sample, wherein the sample separation unit is particularly suitable for separating a blood sample. Specifically, the sample separation unit includes an injection well 211, a plasma/serum well 212, a blood cell well 213, and a storage well 214. The injection groove 211 is used for injecting a sample; the plasma/serum groove 212 is communicated with the injection groove 211, and the plasma/serum groove 212 is used for temporarily storing the separated plasma/serum; the blood cell groove 213 is positioned at one side of the plasma/serum groove 212 far away from the injection groove 211 and is communicated with the plasma/serum groove 212, and the blood cell groove 213 is used for temporarily storing the separated blood cells; a reservoir 214 communicates with the plasma/serum reservoir 212, the reservoir 214 being used to quantify the amount of sample injected.

Referring to fig. 3, fig. 3 shows a sample separation flow chart of the microfluidic detection chip in the present embodiment. Specifically, a whole blood sample is injected from the injection slot 211 through a sample needle, and then, a detection instrument drives the microfluidic detection chip to rotate along a first direction, so that centrifugal separation of the sample can be realized, the mass of blood cells is larger than that of plasma/serum, the blood cells after centrifugal separation enter the blood cell slot 213 which is far away from the injection slot 211, the plasma/serum after centrifugal separation enters the plasma/serum slot 212 which is near to the injection slot 211, and more than whole blood enters the storage slot 214. The sample separation unit of the utility model can process the whole blood sample with high blood corpuscle volume ratio (plasma/serum: 30%, blood corpuscle 70%).

Optionally, in the present embodiment, the sample area 21 is further provided with a first vent 217, the first vent 217 is communicated with the storage groove 214 through a first vent groove 218, and the sample area 21 can be emptied through the first vent 217 and the first vent groove 218.

Specifically, the injection slot 211, the plasma/serum slot 212 and the blood cell slot 213 are arranged in a straight line and are sequentially disposed along the inner side (the side close to the center of the body 10) of the body 10 to the outer side (the side close to the outer edge of the body 10), the storage slot 214 is located on the first side (the right side in fig. 2) of the straight line formed by the injection slot 211, the plasma/serum slot 212 and the blood cell slot 213, the first microchannel 25 is located on the second side (the left side in fig. 2) of the straight line formed by the injection slot 211, the plasma/serum slot 212 and the blood cell slot 213, and correspondingly, the reagent zone 22 is located on the second side of the straight line formed by the injection slot 211, the plasma/serum slot 212 and the blood cell slot 213 to communicate with the first microchannel 25, so configured, the layout of the entire detection unit 20 can be more compact, and more detection units 20 can be provided under the condition that the surface size of the body 10 is the same, the detection flux of the microfluidic detection chip in the embodiment can be further improved.

Further, the side of the plasma/serum groove 212 far from the injection groove 211 is provided with a connection area 215, the connection area 215 is a concave area which extends from the side of the plasma/serum groove 212 far from the injection groove 211 and has a smaller width than the plasma/serum groove 212, the concave area is positioned on the side of the plasma/serum groove 212 near the detection area 23, and the first micro flow channel 25 is communicated with the plasma/serum groove 212 through the connection area 215, so that when the microfluidic detection chip rotates in the second direction (the direction opposite to the first direction), serum or plasma in the plasma/serum groove 212 is collected in the connection area 215, and the plasma or serum in the plasma/serum groove 212 is more suitable for being fully, completely and quickly conveyed to the reagent area 22, so that the usage amount of the sample can be reduced to a certain extent.

Optionally, the sample separation unit further includes a first connection channel 216, the blood cell 213 is connected to the connection region 215 through the first connection channel 216, and a depth of the first connection channel 216 (a depth in a thickness direction of the body 10) and a depth of the first micro flow channel 25 (a depth in a thickness direction of the body 10) are smaller than a depth of the connection region 215 (a depth in a thickness direction of the body 10), specifically, a depth of the connection region 215 is deeper than a depth of the first micro flow channel 25 and the first connection channel 216 by at least 0.5mm, for example, 0.5mm, 1mm, and the like, so as to form a partition region between the first micro flow channel 25 and the connection region 215 and between the first connection channel 216 and the connection region 215, thereby preventing a sample from being released many times, and improving detection accuracy of the microfluidic detection chip in the present embodiment.

In some embodiments of the present invention, a first micro-fluidic valve (not shown in the figure) is disposed on the first micro-fluidic channel 25, the depth of the first micro-fluidic valve is deeper than that of the first micro-fluidic channel 25, specifically, the depth of the first micro-fluidic valve is deeper than that of the first micro-fluidic channel 25 by at least 1mm, such as 1mm, 1.2mm, 1.4mm, 1.6mm, etc., by disposing the first micro-fluidic valve on the first micro-fluidic channel 25, the sample in the plasma/serum tank 212 can be further prevented from being released into the reagent zone 22 for multiple times, the liquid in the reagent zone 22 can be further prevented from flowing back into the sample zone 21, and the detection precision of the micro-fluidic detection chip can be further improved.

In the present embodiment, the first microchannel 25 is disposed in a U-shape or a V-shape, and the connection region 215 is closer to the center of the body 10 than the main body region (i.e. the reagent groove 221) of the reagent region 22, and thus is more suitable for establishing a siphon structure to transport the sample in the plasma/serum groove 212 to the reagent region 22 through the first microchannel 25.

Referring again to fig. 1 and 2, reagent zone 22 in this embodiment includes a reagent reservoir 221 and at least two reagent injection sites 222, where reagent injection sites 222 are in communication with reagent reservoir 221. Specifically, the reagent injection sites 222 in this embodiment may be set to be two, three, four, or more than four, and are specifically designed and processed according to actual detection requirements, and by setting at least two reagent injection sites 222 in the reagent region 22, different reagents can be injected from different reagent injection sites 222 in the actual use process of the microfluidic detection chip, so that the contamination rate in the reagent loading process can be reduced, and the detection accuracy of the microfluidic detection chip can be improved.

The drawings in this embodiment show a case when there are two reagent injection sites 222, one of the two reagent injection sites 222 is located on one side of the injection groove 211 close to the center of the body 10 and is communicated with the reagent groove 221 through a fine channel, and the other reagent injection site 222 is located on the inner side of the first micro-channel 25, that is, the first micro-channel 25 arranged in a U-shape or a V-shape is surrounded to form an inner region, so that the reagent injection site 222 is arranged at a position just filling up the blank region of the detection unit 20, the whole detection unit 20 has a compact structure, and is more suitable for arranging a plurality of detection units 20 on the body 10, thereby facilitating the multi-person and multi-item detection of the microfluidic detection chip.

Further, the detection area 23 is located the side that the reagent groove 221 deviates from the center of the body 10 and is communicated with the reagent groove 221 through the second micro flow channel 26, when the micro-fluidic detection chip rotates, the functional reagent and the sample in the reagent groove 221 can flow into the detection area 23 through the second micro flow channel 26, the functional reagent and the sample are mixed and reacted in the detection area 23, and the optical detection can be performed on the detection area 23 through the optical path detection device on the detection instrument.

In the process of performing immunoassay on a sample, under the action of the reagent injection site 222, a magnetic bead antibody and an enzyme-labeled antibody can be sequentially added into the reagent tank 221, the microfluidic detection chip is driven by a detection instrument to rotate and centrifuge, and liquid in the reagent tank 221 is centrifugally conveyed into the detection zone 23 to perform incubation reaction with the sample. After the reaction is finished, the magnetic beads in the detection area are cleaned, at the moment, a magnetic field can be added into the detection area 23 through a detection instrument, the magnetic beads are adsorbed in the detection area 23, then cleaning liquid (shown in figure 4) is injected into the detection area 23 through a reagent injection position 222, a motor on the detection instrument is started again to drive the micro-fluidic detection chip to rotate, at the moment, the rotating speed of the micro-fluidic detection chip is less than 1500 revolutions per minute, after the cleaning liquid enters the detection area 23, the magnetic beads are moved by adding an upper magnetic field and a lower magnetic field to the detection area 23 through the detection instrument, so that the magnetic beads and the cleaning liquid are fully mixed in the detection area 23 to achieve the cleaning effect, then a part of the magnetic field is removed (only the magnetic field for fixing the magnetic beads is reserved), the motor continues to rotate, and the cleaning liquid in the detection area 23 enters the waste liquid storage area 24 through a third micro-channel 27.

However, in the actual cleaning process, sometimes the waste liquid is not completely discharged into the waste liquid storage area 24, in order to completely discharge the liquid in the detection area 23 into the waste liquid storage area 24, the microfluidic detection chip may be emptied, as shown in fig. 5, when the liquid is emptied, the motor of the detection instrument is first used to drive the microfluidic detection chip to rotate at a low speed (the rotation speed is lower than 1500 rpm), and then the motor of the detection instrument is used to drive the microfluidic detection chip to rotate at a high speed (not lower than 3500 rpm), so that the liquid in the detection area 23 can be centrifuged at a high speed to be conveyed into the waste liquid storage area 24 to be emptied. When the volume of the liquid in the detection region 23 is small, the liquid in the detection region 23 does not flow from the third microchannel 27 to the waste liquid storage region 24 during centrifugation, and when the volume of the liquid in the detection region 23 is sufficient, the liquid in the detection region 23 flows from the third microchannel 27 to the waste liquid storage region 24 during centrifugation. Alternatively, the third microchannel 27 in this embodiment is provided with a second microfluidic valve 28, and the depth of the second microfluidic valve 28 is deeper than the depth of the third microchannel 27, specifically, the depth of the second microfluidic valve 28 is deeper than the depth of the third microchannel 27 by at least 1mm, so that the liquid in the waste liquid storage region 24 can be prevented from flowing back to some extent.

In some embodiments, the third micro flow channel 27 has a protruding portion 271 protruding toward the center of the body 10, the distance from the vertex of the protruding portion 271 to the center of the body 10 is not less than the distance from the end of the detection region 23 near the center of the body 10 to the center of the body 10, that is, the tip of the protruding portion 271 is closer to the center of the body 10, and the detection region 23 is farther from the center of the body 10, so that when the magnetic beads in the detection region 23 are cleaned, the detection region 23 can be effectively kept in the detection region 23 during the movement of the microfluidic detection chip, and the cleaning liquid is not easily subjected to centrifugal force to flow from the third micro flow channel 27 into the waste liquid storage region 24, that is, the cleaning liquid can be well mixed with the magnetic beads in the detection region 23 to clean the magnetic beads, and the usage amount of the cleaning liquid in the magnetic bead cleaning process can be reduced.

Referring again to fig. 1 and 2, in some embodiments, the detection unit 20 further includes a buffer region 29, the third micro flow channel 27 is connected between the detection region 23 and the buffer region 29, and the buffer region 29 is communicated with the waste liquid storage region 24 through a second connection channel 201. By providing the buffer area 29 between the waste liquid storage area 24 and the detection area 23, the liquid in the waste liquid storage area 24 can be prevented from flowing back into the detection area 23. It should be noted that the second connecting channel 201 is a thin channel, and the depth of the second connecting channel 201 is smaller than the depth (depth along the thickness direction of the body 10) of the waste liquid storage area 24, specifically, the depth of the second connecting channel 201 is at least 0.5mm shallower than the waste liquid storage area 24, so that the liquid in the waste liquid storage area 24 can be prevented from flowing back.

Further, the buffer area 29 communicates with the waste liquid storage area 24 through at least two second connecting passages 201. For example, the buffer area 29 and the waste liquid storage area 24 may communicate with each other through two, three, or more than three second connection channels 201, and the drawings in the present embodiment show a case where the buffer area 29 and the waste liquid storage area 24 are connected through three second connection channels 201. In the actual use of the microfluidic detection chip, the second connection channel 201 is occasionally blocked, and in this embodiment, the buffer area 29 and the waste liquid storage area 24 are communicated through at least two second connection channels 201, so that even if a part of the second connection channels 201 are blocked, the liquid in the buffer area 29 can be transported to the waste liquid storage area 24 through other second connection channels 201, and the structure is stable and reliable.

Further, the detecting unit 20 in this embodiment further includes a second air vent 203 and a second air vent groove 204, the second air vent 203 is communicated with the buffer area 29 through the second air vent groove 204, and the evacuation of the microfluidic detecting chip can be improved through the second air vent 203 and the second air vent groove 204, so as to prevent a high-pressure space from being formed on the microfluidic detecting chip.

In order to effectively store waste liquid such as a washing solution, in the present embodiment, the volume of the waste liquid storage area 24 is set to be larger than the volume of the buffer area 29, wherein the waste liquid storage area 24 is located on the side of the detection area 23 away from the reagent area 22, the waste liquid storage area 24 includes a first edge 241 located near the buffer area 29, the first edge 241 includes a first end area 2411, an intermediate area 2412 and a second end area 2413, the first end area 2411, the intermediate area 2412 and the second end area 2413 are sequentially located along the circumferential direction of the main body 10, the first end area 2411 and the intermediate area 2412 are connected by a first concave area 2414, the intermediate area 2412 and the second end area 2413 are connected by a second concave area 2415, the first concave 2414 and the second concave 2415 are concave towards the direction away from the buffer area 29, the first end area 2411 and the second 2413 are convex towards the buffer area 29, the position of communication of the second connecting passage 201 with the waste liquid reserving section 24 is located in the intermediate region 2412. Thus, in the rotation process of the microfluidic chip, the first end region 2411 and the second end region 2413 protruding toward the buffer region 29 can guide the liquid in the waste liquid storage region 24, and guide the liquid in the waste liquid storage region 24 toward the inside of the waste liquid storage region 24, and meanwhile, the first concave region 2414 and the second concave region 2415 at the two ends of the middle region 2412 can respectively block the liquid flowing from the first end region 2411 and the second end region 2413, so as to prevent the liquid in the waste liquid storage region 24 from flowing toward the middle region 2412, and further prevent the liquid in the waste liquid storage region 24 from flowing to the second connecting channel 201, and further prevent the liquid in the waste liquid storage region 24 from flowing back to the buffer region 29.

Specifically, the waste liquid storage area 24 in this embodiment further includes a second edge 242, the second edge 242 is located on a side of the waste liquid storage area 24 close to the outer edge of the body 10, the second edge 242 is an arc-shaped edge, and the arc-shaped edge is parallel to the outer edge of the body 10, so that the miniaturization design of the body 10 is facilitated, and the production cost of the microfluidic detection chip can be reduced.

In the actual use process, under the action of centrifugal force, the magnetic beads in the detection region 23 are occasionally transported into the waste liquid storage region 24 and even thrown out toward the direction close to the outer edge of the body 10, when the magnetic beads are thrown out of the waste liquid storage region 24 under the action of centrifugal force, the magnetic beads are clamped between the intermediate layer and the body 10, and under the condition that the centrifugal force is large enough, the magnetic beads move between the intermediate layer and the body 10 and lift the intermediate layer from the body 10, so that liquid leakage occurs. Therefore, the detecting unit 20 in this embodiment further includes a stopping groove 202, the stopping groove 202 is disposed on a side of the waste liquid storing area 24 away from the detecting area 23, and when the magnetic beads are thrown out of the waste liquid storing area 24 under the action of centrifugal force, the magnetic beads can be accommodated in the stopping groove 202 located outside the waste liquid storing area 24, so as to prevent the magnetic beads from continuously moving toward the outer edge of the body 10 to lift the middle layer, and finally achieve the effect of preventing liquid leakage.

Optionally, the stopping groove 202 in this embodiment extends along the circumferential direction of the body 10, and the length of the stopping groove 202 is not less than the length of the waste liquid storage area 24 along the circumferential direction of the body 10, that is, the length of the stopping groove 202 along the circumferential direction of the body 10 is equal to or greater than the length of the waste liquid storage area 24 along the circumferential direction of the body 10, so that the magnetic bead flowing out from the waste liquid storage area 24 is stopped and contained in the stopping groove 202, the magnetic bead does not lift the middle layer from the body 10, and the microfluidic chip is not prone to leakage.

From the above description, it can be seen that the above-mentioned embodiments of the present invention achieve the following technical effects: because the utility model provides a be provided with a plurality of detecting element on the micro-fluidic detection chip, each detecting element's simple structure only needs to control detecting instrument's slew velocity and direction etc. and can realize the separation of sample, can realize whole blood and go up the appearance, can also realize releasing according to the preface of functional reagent, sample etc.. Meanwhile, the microfluidic detection chip can realize multi-person multi-item detection, has a simple structure, and can reduce the production and manufacturing cost and the detection cost of the microfluidic detection chip. Furthermore, the utility model provides a little flow control detects chip can practice thrift the quantity of washing liquid and be difficult to the weeping problem that appears.

Spatially relative terms, such as "above … …," "above … …," "above … … surface," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

It should be noted that the terms "first", "second", and the like are used to define the components, and are only used for convenience of distinguishing the corresponding components, and if not stated otherwise, the terms have no special meaning, and therefore, the scope of the present invention should not be construed as being limited.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A microfluidic detection chip is characterized by comprising a sheet-shaped body (10) and a plurality of detection units (20) arranged on the body (10), wherein the detection units (20) are radially arranged on the body (10), and the detection units (20) comprise:

a sample area (21), the sample area (21) being at least for injection of a sample; a reagent zone (22), the reagent zone (22) being for performing injection of a functional reagent, the reagent zone (22) comprising a reagent reservoir (221), the reagent reservoir (221) being in communication with the sample zone (21) through a first micro flow channel (25);

a detection area (23), wherein the detection area (23) is used for carrying out a mixing reaction of a functional reagent and a sample, and the detection area (23) is communicated with the reagent area (22) through a second micro-channel (26); and

and the waste liquid storage area (24) is used for containing the reacted functional reagent and the sample, and the waste liquid storage area (24) is communicated with the detection area (23) through a third micro-channel (27).

2. The microfluidic detection chip according to claim 1, wherein the sample area (21) comprises a sample separation unit comprising:

an injection groove (211), wherein the injection groove (211) is used for injecting a sample;

a plasma/serum tank (212), wherein the plasma/serum tank (212) is communicated with the injection tank (211), and the plasma/serum tank (212) is used for temporarily storing the separated plasma/serum;

the blood cell groove (213), the blood cell groove (213) is positioned on one side of the plasma/serum groove (212) far away from the injection groove (211) and is communicated with the plasma/serum groove (212), and the blood cell groove (213) is used for temporarily storing the separated blood cells; and

a reservoir (214), the reservoir (214) in communication with the plasma/serum reservoir (212), the reservoir (214) for quantifying a sample infusion volume.

3. The microfluidic detection chip according to claim 2, wherein a side of the plasma/serum channel (212) away from the injection channel (211) is provided with a connection region (215), and the first micro-channel (25) is communicated with the plasma/serum channel (212) through the connection region (215);

the sample separation unit further comprises a first connection channel (216), the blood cell groove (213) is communicated with the connection area (215) through the first connection channel (216), and the depths of the first connection channel (216) and the first micro flow channel (25) are smaller than the depth of the connection area (215).

4. The microfluidic detection chip according to claim 1, wherein the first microchannel (25) is provided with a first microfluidic valve having a depth at least 1mm deeper than the depth of the first microchannel (25); and/or the presence of a gas in the gas,

a second miniflow valve (28) is arranged on the third miniflow channel (27), and the depth of the second miniflow valve (28) is at least 1mm deeper than that of the second miniflow channel (26).

5. The microfluidic detection chip according to claim 1, wherein the detection unit (20) further comprises a buffer region (29), the third microchannel (27) is connected between the detection region (23) and the buffer region (29), and the buffer region (29) is communicated with the waste solution storage region (24) through a second connection channel (201).

6. The microfluidic detection chip according to claim 5, wherein the buffer region (29) is in communication with the waste liquid storage region (24) through at least two of the second connection channels (201).

7. The microfluidic detection chip according to claim 5, wherein the volume of the waste liquid storage region (24) is larger than the volume of the buffer region (29), wherein,

the waste liquid storage area (24) is located on the side of the detection area (23) far away from the reagent area (22), the waste liquid storage area (24) comprises a first edge (241) arranged close to the buffer area (29), the first edge (241) comprises a first end area (2411), a middle area (2412) and a second end area (2413), the first end area (2411), the middle area (2412) and the second end area (2413) are arranged in sequence along the circumferential direction of the body (10), the first end area (2411) and the middle area (2412) are connected through a first concave area (2414), the middle area (2412) and the second end area (2413) are connected through a second concave area (2415), and the first concave area (2414) and the second concave area (2415) are concave towards the direction far away from the buffer area (29), the first end region (2411) and the second end region (2413) are convex toward the direction close to the buffer area (29), and the position of communication of the second connecting passage (201) with the waste liquid storage area (24) is located in the intermediate region (2412).

8. The microfluidic detection chip according to any one of claims 1 to 7, wherein the detection unit (20) further comprises a stop groove (202), and the stop groove (202) is disposed on a side of the waste liquid storage region (24) away from the detection region (23).

9. The microfluidic detection chip according to claim 8, wherein the stop groove (202) extends along a circumferential direction of the body (10), and a length of the stop groove (202) is not less than a length of the waste liquid storage region (24) along the circumferential direction of the body (10).

10. The microfluidic detection chip according to any one of claims 1 to 7, wherein the third microchannel (27) has a convex portion (271) that is convex toward the center of the body (10), and the distance from the vertex of the convex portion (271) to the center of the body (10) is not less than the distance from the end of the detection region (23) near the center of the body (10) to the center of the body (10).