CN217015422U

CN217015422U - Novel chromatography hollow column

Info

Publication number: CN217015422U
Application number: CN202122950374.8U
Authority: CN
Inventors: 叶伟
Original assignee: Wuhan Chunyuan Biotechnology Co ltd
Current assignee: Wuhan Chunyuan Biotechnology Co ltd
Priority date: 2021-11-29
Filing date: 2021-11-29
Publication date: 2022-07-22
Anticipated expiration: 2031-11-29

Abstract

The utility model belongs to the technical field of the empty post of chromatography, a novel empty post of chromatography is disclosed, include by lower extreme lower end cover, column casing, upper end cover that sets gradually and be located upper sieve plate and the lower sieve plate of both sides about in the column casing, still including the seal assembly who is used for the shutoff upper end cover, detachably installs on the inner wall that the upper end cover is close to column casing one side and links up the ring, the bottom annular that links up the ring is provided with four screw rods, four the screw rod wears to establish sieve and lower sieve plate in proper order, the bottom of going up sieve and sieve down all is provided with a plurality of nuts, and threaded connection between nut and the screw rod, the bottom in the column casing is provided with flow control assembly. When needing to wash upper screen plate and lower sieve, through the screw-thread fit between upper end cover and the column casing, pull down the upper end cover and take out from the column casing through screw rod and nut drive upper screen plate and lower sieve, pull down the back with the nut from the screw rod to easy taking down upper screen plate and lower sieve and wash.

Description

Novel chromatography hollow column

Technical Field

The application relates to the technical field of chromatography hollow columns, in particular to a novel chromatography hollow column.

Background

The chromatographic empty column is specially designed for antigen antibody, recombinant protein purification, protein desalination and the like, can be filled with chromatographic media with separation mechanisms such as immunoaffinity, ion exchange, gel filtration, reverse phase and the like according to requirements, and is a powerful tool for purifying antibodies, proteins and other molecules.

The chromatography hollow column is an experimental consumable material capable of being used repeatedly, and after repeated use, the chromatography hollow column needs to be cleaned for sustainable use of the chromatography hollow column, but a sieve plate and a column tube in the existing chromatography hollow column are designed in an integrated mode, so that the cleaning is troublesome, and a novel chromatography hollow column is necessary to be developed based on the design.

SUMMERY OF THE UTILITY MODEL

In order to solve sieve and the column casing in the empty post of current chromatography and adopt the integrated into one piece design for wash comparatively trouble problem, this application provides novel empty post of chromatography.

The application provides a novel chromatography hollow column adopts following technical scheme:

novel chromatography hollow column, include by lower extreme cover, column casing, upper end cover that supreme set gradually down and lie in column casing upper and lower both sides the last sieve plate and the lower sieve plate, still including the seal assembly who is used for the shutoff upper end cover, detachably installs on the inner wall that the upper end cover is close to column casing one side and links up the ring, the bottom annular of linking up the ring is provided with four screws, four the screw rod wears to establish sieve and lower sieve plate in proper order, the bottom of going up sieve and sieve down all is provided with a plurality of nuts, and threaded connection between nut and the screw rod, the bottom in the column casing is provided with flow control assembly.

Furthermore, the top center of the upper end cover is provided with a through hole convenient for liquid injection in a penetrating manner, and the inner wall of the upper end cover is provided with an internal thread in threaded connection with the column tube.

Furthermore, seal assembly includes the rubber buffer and keeps off the ring, there is interference fit between rubber buffer and the through-hole, keep off ring fixed connection at the top of rubber buffer, and keep off and contradict each other between ring and the upper end cover, keep off the lug that the top center fixedly connected with of ring is convenient for install or dismantle the rubber buffer.

Furthermore, be provided with the scale mark that convenient to use person read the interior liquid volume of column tube on the outer wall of column tube, the material of screw rod and nut is plastics.

Further, the flow regulation subassembly includes first ring and second ring, the laminating of first ring is at the top of second ring, and first ring and second ring all rotationally set up the bottom in the pillar pipe.

Further, all run through on first ring and the second ring and seted up a plurality of water conservancy diversion holes that the size is the same, fixedly connected with links up the piece on the outer wall of first ring, set up on the outer wall of column tube and be convenient for link up the piece and rotate ground notch, link up one side fixedly connected with arc that first ring was kept away from to the piece, and the arc is laminated with the column tube outer wall mutually.

To sum up, the application comprises the following beneficial technical effects:

when the upper sieve plate and the lower sieve plate need to be cleaned, the upper end cover is detached through the thread fit between the upper end cover and the column tube, the upper sieve plate and the lower sieve plate are driven to be taken out of the column tube through the screw rod and the nut, and the upper sieve plate and the lower sieve plate are easily detached to be cleaned after the nut is detached from the screw rod;

the arc-shaped plate drives the connecting block to rotate, and the connecting block drives the first circular ring to rotate, so that the flow guide holes formed in the first circular ring and the second circular ring are staggered or penetrated mutually, and the flow rate of liquid flowing out of the column tube is adjusted.

Drawings

FIG. 1 is a schematic diagram of an axial structure of the present application;

FIG. 2 is a cross-sectional structural schematic of the present application;

fig. 3 is a schematic diagram of the explosive structure of the present application.

The reference numbers in the figures illustrate:

1. a lower end cover; 2. a column tube; 3. an upper end cover; 301. a through hole; 4. an upper sieve plate; 5. a lower sieve plate; 6. An adaptor ring; 7. a screw; 8. a nut; 9. a rubber plug; 10. a baffle ring; 11. a bump; 12. a first circular ring; 13. a second circular ring; 14. a joining block; 15. an arc-shaped plate.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application; it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments, and all other embodiments obtained by those of ordinary skill in the art without any inventive work based on the embodiments in the present application belong to the protection scope of the present application.

In the description of the present application, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.

Example (b):

the present application is described in further detail below with reference to figures 1-3.

The embodiment of the application discloses novel chromatography hollow column, include by lower supreme lower end cover 1 that sets gradually, column casing 2, upper end cover 3 and the last sieve plate 4 and the lower sieve plate 5 that are located column casing 2 interior upper and lower both sides, still including the seal assembly who is used for shutoff upper end cover 3, detachably installs linking ring 6 on the inner wall that upper end cover 3 is close to column casing 2 one side, the bottom annular of linking ring 6 is provided with four screw rods 7, four screw rods 7 wear to establish in proper order sieve 4 and lower sieve plate 5, the bottom of going up sieve plate 4 and lower sieve plate 5 all is provided with a plurality of nuts 8, and threaded connection between nut 8 and the screw rod 7. More specifically, when the upper sieve plate 4 and the lower sieve plate 5 need to be cleaned, the upper end cover 3 is detached and the upper sieve plate 4 and the lower sieve plate 5 are driven by the screw 7 and the nut 8 to be taken out of the column tube 2 through the thread fit between the upper end cover 3 and the column tube 2, and the nut 8 is detached from the screw 7, so that the upper sieve plate 4 and the lower sieve plate 5 can be easily detached for cleaning.

The through-hole 301 of seting up the liquid of being convenient for to annotate is run through at the top center of upper end cover 3, the internal thread with 2 threaded connection of pillar pipe is seted up to the inner wall of upper end cover 3, sealing assembly includes rubber buffer 9 and fender ring 10, there is interference fit between rubber buffer 9 and the through-hole 301, keep off ring 10 fixed connection at the top of rubber buffer 9, and keep off and contradict each other between ring 10 and the upper end cover 3, keep off the lug 11 that the central fixedly connected with in top of ring 10 was convenient for install or dismantle rubber buffer 9, be provided with the scale mark that convenient to use person read the liquid volume in the pillar pipe 2 on the outer wall of pillar pipe 2, the material of screw rod 7 and nut 8 is plastics.

The bottom in the column casing 2 is provided with the flow control assembly, the flow control assembly includes first ring 12 and second ring 13, the laminating of first ring 12 is at the top of second ring 13, and first ring 12 and second ring 13 all rotationally set up the bottom in column casing 2, all run through on first ring 12 and the second ring 13 and seted up a plurality of water conservancy diversion holes that the size is the same, fixedly connected with links up piece 14 on the outer wall of first ring 12, it is convenient for link up 14 rotation ground notch to link up piece 14 to have been seted up on the outer wall of column casing 2, link up one side fixedly connected with arc 15 that piece 14 kept away from first ring 12, and arc 15 and the outer wall of column casing 2 laminate mutually. More specifically, the arc-shaped plate 15 drives the connecting block 14 to rotate, and the connecting block 14 drives the first circular ring 12 to rotate, so that the flow guide holes formed in the first circular ring 12 and the second circular ring 13 are staggered or penetrated mutually, and the flow rate of liquid flowing out of the column tube 2 is adjusted.

The implementation principle of the novel chromatography hollow column in the embodiment of the application is as follows: in the using process, firstly, the connecting ring 6 is arranged on the inner wall of the upper end cover 3 close to one side of the column tube 2, secondly, the upper sieve plate 4 and the lower sieve plate 5 sequentially pass through the screw 7, the upper sieve plate 4 and the lower sieve plate 5 are adjusted in the height position in the column tube 2 through the mutual matching between the nut 8 and the screw 7, then, the upper sieve plate 4 and the lower sieve plate 5 are arranged in the column tube 2 through the thread matching between the upper end cover 3 and the column tube 2, when the upper sieve plate 4 and the lower sieve plate 5 need to be cleaned, the upper end cover 3 is detached through the thread matching between the upper end cover 3 and the column tube 2, the upper sieve plate 4 and the lower sieve plate 5 are driven to be taken out from the column tube 2 through the screw 7 and the nut 8 is detached from the screw 7, so that the upper sieve plate 4 and the lower sieve plate 5 are easily detached for cleaning, which is convenient, the connecting block 14 is driven to rotate through the arc-shaped plate 15, the joining block 14 drives the first ring 12 to rotate, so that the diversion holes formed in the first ring 12 and the second ring 13 are staggered or penetrated with each other, and the outflow rate of the liquid in the column tube 2 is adjusted.

The above are preferred embodiments of the present application, and the scope of protection of the present application is not limited thereto, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. Novel chromatography hollow column, include by lower end cover (1), column casing (2), upper end cover (3) that supreme set gradually down and be located column casing (2) upper and lower both sides go up sieve (4) and lower sieve (5), its characterized in that: still including the seal assembly who is used for shutoff upper end cover (3), detachably installs link ring (6) on upper end cover (3) is close to the inner wall of column tube (2) one side, the bottom annular of link ring (6) is provided with four screw rods (7), four last sieve (4) and lower sieve plate (5) are worn to establish in proper order in screw rod (7), the bottom of going up sieve (4) and lower sieve plate (5) all is provided with a plurality of nuts (8), and threaded connection between nut (8) and screw rod (7), bottom in column tube (2) is provided with flow control assembly.

2. The novel chromatographic empty column according to claim 1, characterized in that: the top center of upper end cover (3) runs through and sets up through-hole (301) of being convenient for annotate the liquid, the internal thread with column tube (2) threaded connection is seted up to the inner wall of upper end cover (3).

3. The novel chromatographic empty column according to claim 2, characterized in that: sealing assembly includes rubber buffer (9) and keeps off ring (10), there is interference fit between rubber buffer (9) and through-hole (301), keep off ring (10) fixed connection at the top of rubber buffer (9), and keep off and contradict each other between ring (10) and upper end cover (3), keep off lug (11) that the top center fixedly connected with of ring (10) is convenient for install or dismantle rubber buffer (9).

4. The novel chromatographic empty column according to claim 1, characterized in that: be provided with the scale mark that the person of being convenient for read the interior liquid volume of column tube (2) on the outer wall of column tube (2), the material of screw rod (7) and nut (8) is plastics.

5. The novel hollow chromatographic column according to claim 1, wherein: the flow regulation assembly comprises a first circular ring (12) and a second circular ring (13), wherein the first circular ring (12) is attached to the top of the second circular ring (13), and the first circular ring (12) and the second circular ring (13) are both rotatably arranged at the bottom in the column tube (2).

6. The novel hollow chromatographic column according to claim 5, characterized in that: all run through on first ring (12) and second ring (13) and seted up the water conservancy diversion hole that a plurality of sizes are the same, fixedly connected with links up piece (14) on the outer wall of first ring (12), it is convenient for link up piece (14) rotation ground notch to have seted up on the outer wall of column tube (2), link up one side fixedly connected with arc (15) of first ring (12) are kept away from to piece (14), and arc (15) laminate mutually with column tube (2) outer wall.