CN216916905U

CN216916905U - Multi-chamber solution sample storage and transportation device

Info

Publication number: CN216916905U
Application number: CN202220784391.0U
Authority: CN
Inventors: 宫楠
Original assignee: Shandong Ruiang Biotechnology Co ltd
Current assignee: Shandong Ruiang Biotechnology Co ltd
Priority date: 2022-04-02
Filing date: 2022-04-02
Publication date: 2022-07-08
Anticipated expiration: 2032-04-02

Abstract

The utility model provides a multi-chamber solution sample storage and transportation device, comprising: the solution storage and transportation device is provided with a plurality of independent solution storage and transportation devices which are arranged in parallel and communicated with the cavity, and a liquid inlet is arranged on the solution storage and transportation device and is connected with a first port of the multi-way mixing pipeline. The cell freezing and storing device with multiple chambers, separable use, rapid liquid injection and lower pollution risk is provided.

Description

Multi-chamber solution sample storage and transportation device

Technical Field

The utility model belongs to the field related to medical machinery, and particularly relates to a multi-chamber solution sample storage and transportation device.

Background

The statements in this section merely provide background information related to the present disclosure and may not necessarily constitute prior art.

Cell cryopreservation is one of the main methods for long-term storage of cells, and at present, the cells can be placed in liquid nitrogen at the temperature of 196 ℃ below zero by utilizing a specific cryopreservation technology for cryopreservation, so that the cells can be temporarily separated from a growth state and the cell characteristics can be preserved, and the cells are recovered for experiments when needed. Once the cells leave the living body and start primary culture, the biological characteristics of the cells are gradually changed along with the increase of the passage number, a certain amount of cells are properly preserved, the special performance of the cells for a certain passage number can be preserved at a proper time, and the cells can be prevented from being lost due to pollution or other accidents of the cultured cells, so that the effect of preserving the cells is achieved. When the cells are frozen, the corresponding cryoprotectant can be adopted according to the characteristics of the cells, and the main components of the cryoprotectant are glycerol or dimethyl sulfoxide, a culture medium, serum and the like. At present, after the cryoprotectant is added into the cells, the cells can be put into liquid nitrogen (196 ℃ below zero) for long-term storage by adopting two modes of programmed cooling or vitrification cooling. When the stored cells need to be subjected to rewarming culture, the cells are taken out of the liquid nitrogen and placed into a water bath at 37 ℃ for thawing, then the cryoprotectant is removed, and the cells are suspended in a cell culture container for rewarming culture. In the processes of cell freezing and rewarming, higher requirements are put forward on the low temperature resistance, organic solvent resistance and non-rupture performance of freezing consumables adopted by cell freezing and rewarming.

At present, the storage and transportation devices mainly used in the cell cryopreservation process are a cryopreservation tube and a cryopreservation bag. The volume of the cryopreservation tube is limited, and the cryopreservation tube is mostly used for cryopreservation of a lower number of cells in a laboratory and has 2ml-10ml different volumes; the cryopreservation bag is gradually favored by the fields of stem cell storage, cell therapy and the like due to the large storage volume, the relative volume is large, the volume is different from 25 ml to 250ml, a special liquid adding pipeline is arranged, the sealing performance is good after hot melting sealing, and the cryopreservation bag has great advantages in the process of preserving a large number of cells. However, at present, a cell sample storage and transportation device commonly used in the market is a single chamber, that is, all cells are frozen in a single space, so that if the operation is improper or the storage and transportation device is broken, the whole sample is discarded, and meanwhile, if the cell sample storage and transportation device is used for rewarming, the using amount does not need the amount of the whole frozen sample, the single chamber storage and transportation device can only be used for rewarming once, and the unused cell sample can be wasted.

SUMMERY OF THE UTILITY MODEL

The utility model provides a multi-chamber cell sample storage and transportation device for solving the problems that a common single-chamber cell sample storage and transportation device is high in sample damage risk, can only be subjected to single temperature recovery and cannot be used for backup, and precious cell samples are wasted.

According to some embodiments, the utility model adopts the following technical scheme:

a multi-chambered, solution-specimen storage and transportation device, comprising: the solution storage and transportation device is provided with a plurality of independent solution storage and transportation devices which are arranged in parallel and communicated with the cavity, and a liquid inlet is arranged on the solution storage and transportation device and is connected with a first port of the multi-way mixing pipeline.

In addition, the multi-chamber solution sample storage and transportation device according to the embodiment of the utility model can also have the following additional technical characteristics:

preferably, the bottom of any adjacent chamber is provided with a connecting pipeline communicated with each other.

Preferably, a liquid outlet is arranged at the top of the chamber.

Preferably, the first pipeline is connected with the liquid inlet and the first port, and a liquid adding valve is arranged on the first pipeline.

Preferably, a stop valve is arranged on a second pipeline connected with a second port of the multi-way mixing pipeline, and the cavity is filled with liquid through the second pipeline.

Preferably, a cutoff switch is arranged on a third pipeline connected with a third port of the multi-way mixing pipeline.

Preferably, a press cutting line capable of separating the chambers from each other is provided between any adjacent chambers.

Preferably, the connecting line can be sealed by a heat sealing machine.

Preferably, the multi-way mixing pipeline can be a three-way pipeline.

Preferably, the solution storage and transportation device is made of EVA materials.

Compared with the prior art, the utility model has the beneficial effects that:

the utility model carries out the operations of vacuumizing and liquid adding of the liquid sample by the design of the multi-way mixing pipeline, and the liquid sample is automatically sucked into each chamber by utilizing negative pressure, thereby avoiding the pollution caused by residual air and the formation of bubbles.

The liquid sample storage and transportation device is characterized in that the liquid sample storage and transportation device comprises a liquid sample storage tank, a liquid sample conveying pipe and a liquid sample conveying pipe. When the liquid sample is stored and transported, if the storage and transportation device is damaged, the risk that the precious liquid sample is completely polluted and abandoned can be avoided. When the liquid sample is used, the time and the number of the samples can be flexibly selected, and the risk that the precious liquid sample can be used only once and is used up is avoided.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the utility model, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the utility model and together with the description serve to explain the utility model and not to limit the utility model.

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of the main body of the solution storage and transportation device of the present invention;

FIG. 3 is a plan view of the main body of the solution storage and transportation device of the present invention;

fig. 4 is a side view of the body of the solution storage and transportation device of the present invention.

Description of reference numerals:

1. a solution storage and transportation device, 2, air suction and liquid injection pipelines, 3, a stop valve, 4, a second pipeline, 5, a third pipeline, 6, a three-way pipeline, 7, a liquid adding valve, 8, a first pipeline, 9, a cavity, 10, a connecting pipeline, 11, a pressing cutting line, 12, a liquid inlet, 13, a liquid outlet, 14 and a stop switch

The specific implementation mode is as follows:

the utility model is further described with reference to the following figures and examples.

It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the utility model as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

Example one

As shown in fig. 1 to 4, the present invention provides a multi-chamber solution sample storage and transportation device, comprising: the solution storage and transportation device is provided with a plurality of solution storage and transportation devices 1 which are arranged in parallel and are independent and communicated with a cavity 9, wherein a liquid inlet 12 is arranged on the solution storage and transportation device 1, and the liquid inlet 12 is connected with a first port of a multi-way mixing pipeline.

In the present invention, the solution storage and transportation device 1 can store cell samples or other precious active solutions, and the number, volume and temperature range of the chambers in the solution storage and transportation device 1 are not particularly limited and can be selected according to the actual application.

In a specific embodiment, the solution storage and transportation device 1 is used for storing and transporting cell samples, and the solution storage and transportation device 1 may be a cell storage and transportation bag processed by EVA. By controlling the heat seal of the channels between the cavities, 1 to 5 samples can be distributed, the volume of the samples can be from 3ml to 30ml, and the selection is flexible. Taking 5 parts for storage and transportation as an example, five samples can be completed by one-time operation during filling, so that the operation steps and the pollution risks of sample exposure are reduced, and the problem of batch-to-batch difference caused by batch filling of the samples is thoroughly solved. When the sample is stored and transported, if the original product is damaged, the sample is completely polluted and damaged, and after the damaged part of the cell storage and transportation bag of the novel storage and transportation device is damaged, the cavity without damage can still be continuously and normally used. When sampling, the original product can only use all samples once, and the cell storage and transportation bag of the novel storage and transportation device can be used for using the same sample in batches for many times.

In this embodiment, the bottom of any adjacent chamber 9 is provided with a connecting pipeline 10 which is communicated with each other, and after the injection solution enters one chamber 9 through the loading port 12, the injection solution enters the other chambers 9 through the connecting pipeline 10. In addition, the connecting pipes 10 can be heat-sealed, and the connecting pipes 10 between the chambers 9 are respectively heat-sealed by a heat-sealing machine to form the chambers 9 which are independent and not communicated with each other.

In this embodiment, liquid outlets 13 are disposed at the top of the chambers 9, each chamber 9 corresponds to one liquid outlet 13, and the solution stored inside the chamber 9, such as cell sap, can be output through the respective liquid outlet 13.

As an alternative embodiment, a closed diaphragm is disposed at a port of the liquid outlet 13, a cover is hermetically connected to an upper end of the closed diaphragm, a sterile cavity is formed between the cover and the closed diaphragm, the cover can be separated from the closed diaphragm, after the cover is separated from the closed diaphragm, the cavity of the storage and transportation device remains sealed, and a syringe can be inserted into the closed diaphragm to perform corresponding operations.

In this embodiment, a liquid inlet 12 is formed at a position close to the bottom of one side of the solution storage and transportation device 1, the liquid inlet 12 is connected with one end of the first pipeline 8, and the other end of the first pipeline 8 is connected with a multi-way mixing pipeline. A filling valve 7 is arranged on the first line 8, and syringes are added from the filling valve 7 to fill the chambers 9 of the solution storage and transportation device 1 with the components to be added.

And a stop valve 3 is arranged on a second pipeline 4 connected with a second port of the multi-way mixing pipeline, and the cavity 9 is injected with liquid through the second pipeline 4. A stop switch 14 is arranged on a third pipeline 5 connected with a third port of the multi-way mixing pipeline, and the chamber 9 is vacuumized through the third pipeline 5.

In a specific embodiment, the multi-way mixing pipe may be a three-way pipe, a first end of which is connected to one end of the first pipe 8; the second end of the three-way pipeline is connected with one end of the second pipeline 4, the stop valve 3 is arranged on the second pipeline 4, the stop valve 3 on the second pipeline 4 is opened, the cell container is butted by the luer at the tail end of the second pipeline 4, liquid injection operation is carried out on the cavity 9, and the stop valve 3 on the second pipeline 4 is closed after the liquid injection operation is finished. The third end of the three-way pipeline is connected with one end of a third pipeline 5, a stop switch 14 is arranged on the third pipeline 5, the stop switch 14 on the third pipeline 5 is opened, meanwhile, the stop valve 3 on the second pipeline 4 is closed, the other end of the third pipeline 5 is connected with an air extraction device (such as an injector) to carry out vacuum-pumping operation on the solution storage and transportation device, and the stop valve 14 is closed after the vacuum-pumping operation is finished.

In the present embodiment, a pressing cutting line 11 capable of separating the chambers from each other is provided between any adjacent chambers 9, and a certain chamber or certain chambers are quickly cut off by the pressing cutting line 11.

In this embodiment, the first pipeline 8, the second pipeline 4 and the third pipeline 5 form an air-extracting and liquid-injecting pipeline 2, the solution storage and transportation device 1 and the air-extracting and liquid-injecting pipeline 2 are mutually independent and can be connected, and after the liquid inlet 12 of the solution storage and transportation device 1 is separated from the air-extracting and liquid-injecting pipeline 2, the liquid inlet 12 can be sealed in a heat sealing mode.

Example two

Adopt embodiment one to provide a room solution sample storage and transportation device, include: the device comprises a plurality of solution storage and transportation devices 1 which are arranged in parallel and are independent and communicated with a cavity 9, wherein a liquid inlet 12 is arranged on the solution storage and transportation devices 1, and the liquid inlet 12 is connected with a first port of a multi-channel mixing pipeline.

The embodiment provides a method for realizing a room solution sample storage and transportation device, which is not limited to a cell solution, and can also be other precious active solutions.

Taking cryopreserved stem cell samples as an example, the method for realizing the path of the indoor solution sample storage and transportation device provided by the embodiment comprises the following steps:

after the stem cell suspension of the cryoprotectant is prepared, the stop valve 3 on the second pipeline 4 is closed under aseptic conditions, the stop switch 14 on the third pipeline 5 is opened, and the tail end of the third pipeline 5 is connected with an air suction device (such as an injector). Vacuumizing the chambers of the first pipeline 8 and the solution storage and transportation device 1 by using an air extractor, then closing a stop switch 14, connecting a luer at the tail end of the second pipeline 4 with a liquid injection device, and preparing the cryoprotectant cell suspension in the liquid injection device. The stop valve 3 on the second pipe 4 is opened, and the cryoprotectant cell suspension is sucked into the freezing chamber 9 by means of the negative pressure in the pipe and in the chamber of the solution storage and transportation device 1. The cryoprotectant cell suspension enters the first chamber from the liquid inlet 12 of the solution storage and transportation device and then sequentially enters the remaining chambers through the communicated connecting pipeline 10. If the stem cell suspension requires the addition of other ingredients during injection into the solution storage device, it can be added through the addition valve 7 via a syringe. And after the liquid flows into the solution storage and transportation device, the liquid inlet 12 of the solution storage and transportation device 1 is thermally sealed with the interface of the first pipeline 8 by using a heat sealing machine. The storage and transportation device 1 and the first pipeline 8 are separated by heat sealing, and a complete and internally communicated integral frozen sample can be obtained. After the cell injection and the heat sealing of the liquid nitrogen freezing device are finished, program cooling or vitrification cooling can be selected according to requirements, and finally the cell sample is stored in a liquid nitrogen tank at the temperature of-196 ℃. When partial cell samples need to be rewarmed, one or more chambers are quickly cut off by means of reserved pressing and cutting openings, the required chambers are taken out of the liquid nitrogen tank, and the rest chamber samples can be continuously stored in the liquid nitrogen for the next use. And (4) carrying out water bath rewarming on the taken-out chamber, and then outputting the recovered cell suspension through a liquid outlet to carry out subsequent application operation of the stem cells.

In the present embodiment, through the above-mentioned operation, one part of stem cells is directly passed through one infusion operation, and is directly and evenly divided into multiple parts (for example, five parts), so that the repeated operation of one time for each part to obtain equal stem cells is reduced, the operation steps are reduced, and the pollution risk is also reduced.

In the present invention, before the sample is frozen, the connecting pipes 10 between the chambers 9 are respectively sealed according to the subsequent use requirement of the solution sample. After the heat seal, the chambers can be cut off from the middle of the heat seal mark by scissors, so that each chamber 9 becomes an independent and disconnected independent chamber. By this procedure, one fraction of stem cells is preserved in multiple aliquots, each of which is a backup of the same cells. In the embodiment, the same stem cell can be additionally used for multiple times, and the cell quality corresponding to each use opportunity is the same, so that multiple emergency life saving opportunities are increased.

The utility model solves the problem of insufficient volume of the traditional cryopreservation tube, and mainly solves the problems that a common single-cavity sample storage and transportation device has higher sample damage risk, can only carry out single temperature recovery and can not keep backup, and precious cell samples are wasted. The cell cryopreservation and storage device with the multiple cavities, which can be used in a split mode, can be used for quickly injecting liquid and is low in pollution risk is provided, a single or multiple using cavities can be selected in the using process of a product, and the total volume can also be flexibly selected according to actual requirements. The material selected by the device can meet the use in various temperature ranges from normal temperature to deep low temperature, and meets the sterile storage under the conditions of wide capacity range, wide temperature difference and temperature change. The high pollution risk of repeated operation is reduced, and the use safety and the use ratio of the precious solution sample are increased. Meanwhile, the device is also suitable for liquid samples and organic solvents and the like which have toxicity or are easy to inactivate at normal temperature and are stored at ultralow temperature.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Although the embodiments of the present invention have been described with reference to the accompanying drawings, it is not intended to limit the scope of the utility model, and it should be understood by those skilled in the art that various modifications and variations can be made without inventive changes in the technical solutions of the present invention.

Claims

1. The multi-chamber solution sample storage and transportation device is characterized by comprising a plurality of solution storage and transportation devices which are arranged in parallel, independent and communicated with chambers, wherein a liquid inlet is formed in each solution storage and transportation device and is connected with a first port of a multi-way mixing pipeline.

2. The multi-chambered, solution specimen storage and transportation device according to claim 1, wherein the bottom of any adjacent chamber is provided with a connecting line that communicates.

3. The multi-chambered, solution sample storage and transportation device according to claim 1, wherein a liquid outlet is provided at the top of the chamber.

4. The multi-chambered, solution sample storage and transportation device according to claim 1, wherein a first tube connecting said inlet port and said first port is provided with a filling valve.

5. The multi-chambered, solution sample storage and transportation device according to claim 1, wherein a shut-off valve is provided on a second tube connected to a second port of the multi-port mixing tube, and the chamber is filled with liquid through the second tube.

6. The multi-chamber sample solution storage and transportation device according to claim 1, wherein a cutoff switch is disposed on a third pipeline connected to a third port of the multi-way mixing pipeline.

7. The multi-chambered, solution sample storage and transportation device according to claim 1, wherein a pressed trim line is provided between any adjacent chambers to separate the chambers from each other.

8. A multi-chambered, solution sample storage and transportation device according to claim 2, wherein said connecting line is sealable by a heat-sealing machine.

9. A multi-chambered, solution sample storage and transportation apparatus according to claim 1, wherein said multi-way mixing tube is a three-way tube.

10. The multi-chambered, solution specimen storage and transportation device according to claim 1, wherein the solution specimen storage and transportation device is made of EVA material.