CN216900568U - Colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis - Google Patents
Colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis Download PDFInfo
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- CN216900568U CN216900568U CN202121645036.7U CN202121645036U CN216900568U CN 216900568 U CN216900568 U CN 216900568U CN 202121645036 U CN202121645036 U CN 202121645036U CN 216900568 U CN216900568 U CN 216900568U
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 52
- 238000001514 detection method Methods 0.000 title claims abstract description 36
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 title claims abstract description 28
- 239000005985 Paclobutrazol Substances 0.000 title claims abstract description 27
- 239000000447 pesticide residue Substances 0.000 title claims abstract description 14
- 239000012528 membrane Substances 0.000 claims abstract description 34
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 32
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 32
- 239000002250 absorbent Substances 0.000 claims abstract description 16
- 230000002745 absorbent Effects 0.000 claims abstract description 15
- 241000283707 Capra Species 0.000 claims description 9
- 239000000427 antigen Substances 0.000 claims description 9
- 102000036639 antigens Human genes 0.000 claims description 9
- 108091007433 antigens Proteins 0.000 claims description 9
- 239000010931 gold Substances 0.000 claims description 6
- 229910052737 gold Inorganic materials 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 5
- 238000000576 coating method Methods 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 4
- 241001448424 Ophiopogon Species 0.000 claims description 3
- 229930195210 Ophiopogon Natural products 0.000 claims description 3
- 229920000915 polyvinyl chloride Polymers 0.000 abstract description 9
- 239000004800 polyvinyl chloride Substances 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 3
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 18
- 239000000243 solution Substances 0.000 description 8
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000007865 diluting Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 102000011632 Caseins Human genes 0.000 description 2
- 108010076119 Caseins Proteins 0.000 description 2
- 241001448421 Ophiopogon jaburan Species 0.000 description 2
- 244000248557 Ophiopogon japonicus Species 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- DGLRDKLJZLEJCY-UHFFFAOYSA-L disodium hydrogenphosphate dodecahydrate Chemical compound O.O.O.O.O.O.O.O.O.O.O.O.[Na+].[Na+].OP([O-])([O-])=O DGLRDKLJZLEJCY-UHFFFAOYSA-L 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 239000005648 plant growth regulator Substances 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 2
- 229940038773 trisodium citrate Drugs 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 244000003240 Caesalpinia gilliesii Species 0.000 description 1
- 235000014161 Caesalpinia gilliesii Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 241000012097 Lindera communis Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- -1 casein sodium salt Chemical class 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000006748 scratching Methods 0.000 description 1
- 230000002393 scratching effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229940080237 sodium caseinate Drugs 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000004885 tandem mass spectrometry Methods 0.000 description 1
Images
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis, which consists of a sample pad, a gold-labeled combination pad, a nitrocellulose membrane, absorbent paper, a PVC (polyvinyl chloride) bottom plate, a T line and a C line; the nitrocellulose membrane is attached to the PVC base plate, and a T line and a C line are respectively arranged in the middle of the nitrocellulose membrane; one end of the sample pad is pressed with one end of the gold-labeled combination pad by 2-3mm, and the gold-labeled combination pad is positioned at the bottom of the sample pad; the other end of the gold-labeled combination pad is pressed with one end of the nitrocellulose membrane by 2-3mm, and the nitrocellulose membrane is positioned at the bottom of the gold-labeled combination pad; one end of the absorbent paper is pressed with the other end of the nitrocellulose membrane by 2-3mm, and the nitrocellulose membrane is positioned at the bottom of the absorbent paper. The method has the advantages of simple operation, short detection time, high sensitivity, low cost and no need of large-scale instruments, can detect the result within 20min, can detect the result with the lowest detection limit of 0.5mg/kg in the sample, and is suitable for rapid detection in the basic level field.
Description
Technical Field
The utility model belongs to the technical field of pesticide residue detection, and particularly relates to a colloidal gold rapid detection card for paclobutrazol pesticide residue in traditional Chinese medicine radix ophiopogonis.
Background
The traditional Chinese medicine radix ophiopogonis is a dry root tuber of lilyturf root of lilyturf of liliaceae, is called ophiopogon japonicus, golden-edge broadleaf ophiopogonis, lindera communis, mysorethorn and the like, has the effects of nourishing heart, lung and spleen and stomach, resisting inflammation, reducing blood sugar and the like, has high medicinal value, and is a high economic crop.
In order to increase the yield of ophiopogon japonicus and improve economic benefits, some plant growth regulators are used. Of these, paclobutrazol is typical of such inhibitory plant growth regulators. The mechanism is that the crop dwarfing and tillering are promoted by inhibiting the biosynthesis of gibberellin in plants, and the yield and income increase are further realized.
However, the long-term use of paclobutrazol can cause serious harm to groundwater, environment and human health. According to laws and regulations such as agricultural product quality safety law, pesticide management regulation and notice of the State food and drug administration for strengthening traditional Chinese medicine management, paclobutrazol cannot be used in the planting process of radix ophiopogonis.
The traditional pesticide residue paclobutrazol detection method mainly comprises a gas chromatography, a high performance liquid chromatography and a tandem mass spectrometry, and the methods need to rely on large-scale instruments, have high professional requirements, are expensive, are complicated to operate, have long detection period and are not beneficial to rapid detection and use of basic-level personnel.
Based on the above, the utility model provides a colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis to solve the problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis, aiming at the defects in the prior art, the detection card is simple to operate, short in detection time, high in sensitivity, low in price and suitable for rapid detection of a basic level on site, and can detect results within 20min, and the minimum detection limit in a sample is 0.5 mg/kg.
In order to achieve the purpose, the utility model adopts the following technical scheme:
a colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis consists of a sample pad, a gold-labeled combination pad, a nitrocellulose membrane, absorbent paper, a PVC (polyvinyl chloride) bottom plate, a T line and a C line; the nitrocellulose membrane is attached to the PVC base plate, and a T line and a C line are respectively arranged in the middle of the nitrocellulose membrane; one end of the sample pad is pressed with one end of the gold-labeled combination pad by 2-3mm, and the gold-labeled combination pad is positioned at the bottom of the sample pad; the other end of the gold-labeled combination pad is pressed with one end of the nitrocellulose membrane by 2-3mm, and the nitrocellulose membrane is positioned at the bottom of the gold-labeled combination pad; one end of the absorbent paper is pressed with the other end of the nitrocellulose membrane by 2-3mm, and the nitrocellulose membrane is positioned at the bottom of the absorbent paper.
Preferably, the T line is a paclobutrazol-coated antigen line; the C line is a goat anti-mouse secondary antibody coating line; the T line is close to the gold mark combination pad, and the C line is close to the absorbent paper; the interval between the T line and the C line is 4-6 mm.
Preferably, the T line is coated with paclobutrazol-BSA coupled antigen, and the C line is coated with goat anti-mouse secondary antibody.
Preferably, the sample pad is prepared by soaking a glass cellulose membrane in a treatment solution containing bovine serum albumin, sodium caseinate, tween 20, disodium hydrogen phosphate dodecahydrate, sodium chloride, potassium chloride and potassium dihydrogen phosphate, dehumidifying and drying.
Preferably, the particle size of the colloidal gold is 20-40 nm.
Compared with the prior art, the utility model has the beneficial effects that:
1. the rapid detection card provided by the utility model is simple to operate, short in detection time, high in sensitivity, low in price, free of large-scale instruments and suitable for rapid detection on the basic level site, and the result can be detected within 20min, and the lowest detection limit in a sample can be detected by 0.5 mg/kg.
2. The rapid detection card has low cost, is convenient to produce and is easy to popularize and use in large batch.
Drawings
FIG. 1 is a schematic view of a rapid test card according to the present invention;
FIG. 2 is a schematic top view of the rapid test card of the present invention;
FIG. 3 is a diagram showing the test results of the rapid test card of the present invention.
In the figure, 1-sample pad, 2-gold label combined pad, 3-nitrocellulose membrane, 4-absorbent paper, 5-PVC base plate, 6-T line and 7-C line.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings and embodiments, and it is to be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The test methods or test methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials, unless otherwise indicated, are conventionally obtained commercially or prepared by conventional methods.
As shown in fig. 1-2, the utility model provides a colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in ophiopogon root, which consists of a sample pad 1, a gold-labeled binding pad 2, a nitrocellulose membrane 3, absorbent paper 4, a PVC base plate 5, a T line 6 and a C line 7; the nitrocellulose membrane 3 is attached to the PVC bottom plate 5, and a T line 6 and a C line 7 are respectively arranged in the middle of the nitrocellulose membrane 3; one end of the sample pad 1 is pressed with one end of the gold-labeled bonding pad 2 by 2-3mm, and the gold-labeled bonding pad 2 is positioned at the bottom of the sample pad 1; the other end of the gold-labeled binding pad 2 is pressed with one end of the nitrocellulose membrane 3 by 2-3mm, and the nitrocellulose membrane 3 is positioned at the bottom of the gold-labeled binding pad 2; one end of the absorbent paper 4 is pressed with the other end of the nitrocellulose membrane 3 by 2-3mm, and the nitrocellulose membrane 3 is positioned at the bottom of the absorbent paper 4.
Specifically, the T line is a paclobutrazol coated antigen line; the C line is a goat anti-mouse secondary antibody coating line; the T line is close to the gold mark combination pad, and the C line is close to the absorbent paper; the interval between the T line and the C line is 4-6 mm; the T line is coated with paclobutrazol-BSA coupled antigen, and the C line is coated with goat anti-mouse secondary antibody.
The grain diameter of the colloidal gold is 20-40nm, and a paclobutrazol monoclonal antibody is marked; the method judges whether the detection sample contains paclobutrazol pesticide residues or not by judging the color change at the T line and the C line.
In order to embody the rapidness and convenience of the colloidal gold rapid detection card, the utility model also provides a preparation method of a colloidal gold solution for detecting the card, a gold-labeled binding pad, a nitrocellulose membrane and a sample pad, which comprises the following steps:
(1) preparing a colloidal gold solution: the gold decocting is carried out by adopting the method steps of reducing the chloroauric acid by trisodium citrate, and mixing 1% aqueous solution of chloroauric acid and 1% aqueous solution of trisodium citrate according to the volume ratio of 1: 1-1.5, feeding, and decocting gold to obtain a colloidal gold solution with the particle size of 20-40 nm.
(2) Preparing a gold-labeled bonding pad: adjusting the pH value of the colloidal gold by using 0.1-0.2 mol/L potassium carbonate solution, selecting the optimal pH value, adding 3-6 mu g/mL paclobutrazol monoclonal antibody, and rotationally marking for 30-60 minutes; adding bovine serum albumin with the concentration of 1 percent, sealing for 30 minutes at room temperature, then centrifuging for 30 minutes at the speed of 8000rpm, and taking supernatant fluid for 10 times of concentration and redissolution; and adding the complex solution into the enzyme-labeled holes of the gold-labeled binding pads according to the proportion of 3-5 mu L per hole, and drying at the temperature of 37 ℃ for 2 hours.
(3) Preparation of cellulose Nitrate (NC) membrane: diluting paclobutrazol-BSA antigen to 0.2-0.5 mg/mL by using 0.01-0.02 mol/L PBS buffer solution with pH of 7.4, and coating the paclobutrazol-BSA antigen to a T line position by a film scratching instrument according to 0.75 mu L/cm; similarly, diluting the goat anti-mouse secondary antibody to 0.1-0.45 mg/mL, and coating the goat anti-mouse secondary antibody on a C line position; then dried overnight at 37 ℃ and a relative humidity of < 30%.
(4) Preparation of the sample pad: weighing 2.9g of disodium hydrogen phosphate dodecahydrate, 8.0g of sodium chloride, 2.4g of potassium chloride and 2.0g of potassium dihydrogen phosphate, dissolving the mixture by using 800mL of deionized water, adding 0.5-1% of bovine serum albumin, 0.1-0.3% of casein sodium salt and 0.5% of tween 20, uniformly mixing, and fixing the volume to 1L to obtain the sample pad treatment solution. The sample pad was then soaked in the sample pad treatment solution, removed after 5 minutes, and dried overnight at 37 ℃ at a relative humidity of < 30%.
The utility model relates to a detection method for a rapid detection card, which comprises the following steps:
firstly, pretreatment of a radix ophiopogonis sample:
(1) preparing a sample extractant: diluting analytically pure methanol to 40-70% by using deionized water, and uniformly mixing to obtain a sample extractant;
(2) preparing a sample diluent: the diluent is 0.01-0.02 mol/L PBS buffer solution with pH of 6.5-7.4;
(3) mincing radix Ophiopogonis with a mincing machine;
(4) weighing 0.5g of the crushed sample in a 15mL centrifuge tube;
(5) adding 5mL of sample extractant, violently oscillating for 2 minutes, and centrifuging for 5 minutes at 4000 revolutions at room temperature;
(6) taking 50 mu L of supernatant, adding 950 mu L of sample diluent, diluting and mixing uniformly to obtain the liquid to be detected.
(II) point card detection:
and (3-4) drops of the diluted liquid to be detected are vertically dropped into the sample pad by using a plastic suction pipe, the reaction is carried out for 5-8 minutes at room temperature, and the interpretation result is invalid after more than 20 minutes.
(III) interpretation of results:
as shown in fig. 3:
negative (not detected): the color development of the T line is deeper than or equal to that of the C line, which indicates that the paclobutrazol residue in the sample is lower than the detection limit of the colloidal gold card;
positive (detected): the T line is not developed or is weaker than the C line, which shows that the paclobutrazol in the sample is equal to or higher than the detection limit of the colloidal gold card;
and (4) invalidation: the C line does not develop color, which indicates that the reagent is invalid or the operation is wrong, and the re-detection is recommended after the card is replaced.
In conclusion, the colloidal gold rapid detection card disclosed by the utility model is labeled by coupling of colloidal gold with the particle size of 20-40nm and a paclobutrazol monoclonal antibody, and then a paclobutrazol-BSA antigen is coated on a nitrocellulose membrane (NC membrane) at a T line position, and a goat anti-mouse secondary antibody is coated on a C line position. After the radix ophiopogonis sample liquid to be detected is added, the paclobutrazol and the paclobutrazol-BSA at the T line position in the sample compete to bind the paclobutrazol antibody marked on the colloidal gold. Therefore, the paclobutrazol-BSA combined paclobutrazol antibody at the T line position is inversely proportional to the content of paclobutrazol in the sample, so that the result can be conveniently and quickly interpreted according to the color development change of the T line.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the scope of the present invention, which is intended to cover any modifications, equivalents, improvements, etc. within the spirit and scope of the present invention.
Claims (3)
1. The colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis is characterized by comprising a sample pad (1), a gold-labeled combination pad (2), a nitrocellulose membrane (3), absorbent paper (4), a PVC bottom plate (5), a T line (6) and a C line (7); the nitrocellulose membrane (3) is attached to the PVC bottom plate (5), and a T line (6) and a C line (7) are respectively arranged in the middle of the nitrocellulose membrane (3); one end of the sample pad (1) is pressed with one end of the gold-labeled combination pad (2) by 2-3mm, and the gold-labeled combination pad (2) is positioned at the bottom of the sample pad (1); the other end of the gold-labeled combination pad (2) is pressed with one end of the nitrocellulose membrane (3) by 2-3mm, and the nitrocellulose membrane (3) is positioned at the bottom of the gold-labeled combination pad (2); one end of the absorbent paper (4) is pressed with the other end of the nitrocellulose membrane (3) by 2-3mm, and the nitrocellulose membrane (3) is positioned at the bottom of the absorbent paper (4); the T line (6) is a paclobutrazol coated antigen line; the C line (7) is a goat anti-mouse secondary antibody coating line; the T line (6) is close to the gold mark bonding pad (2), and the C line (7) is close to the absorbent paper (4); the interval between the T line (6) and the C line (7) is 4-6 mm.
2. The colloidal gold rapid detection card for detecting paclobutrazol pesticide residue in ophiopogon root according to claim 1, wherein the T line (6) is coated with paclobutrazol-BSA coupled antigen, and the C line (7) is coated with goat anti-mouse secondary antibody.
3. The colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in ophiopogon root according to any one of claims 1-2, wherein the particle size of the colloidal gold is 20-40 nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121645036.7U CN216900568U (en) | 2021-07-20 | 2021-07-20 | Colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121645036.7U CN216900568U (en) | 2021-07-20 | 2021-07-20 | Colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis |
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| Publication Number | Publication Date |
|---|---|
| CN216900568U true CN216900568U (en) | 2022-07-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121645036.7U Active CN216900568U (en) | 2021-07-20 | 2021-07-20 | Colloidal gold rapid detection card for detecting paclobutrazol pesticide residues in radix ophiopogonis |
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| Country | Link |
|---|---|
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