CN216639478U - Exosome separator - Google Patents

Abstract

The utility model provides an exosome separating device, which comprises a separating box, a placing box and an oscillating mechanism, wherein the side surface of the separating box is provided with a box door, the side surface of the placing box is provided with an end door, the placing box is positioned in the separating box, the oscillating mechanism is used for enabling the placing box to vibrate up and down in the separating box in a reciprocating manner, the oscillating mechanism comprises a motor, a rotating shaft, a tooth-lacking gear, a rack and a supporting plate, the motor is arranged on the side surface of the separating box, the rotating shaft is rotatably connected to the inner bottom of the separating box, the tooth-lacking gear is arranged on the rotating shaft, the rack is slidably connected in the separating box and intermittently meshed with the tooth-lacking gear, the supporting plate is arranged at the top of the rack, the placing box is positioned on the supporting plate, and the vibrating operation of the placing box can be realized by the reciprocating motion that the placing box firstly moves upwards and then moves downwards through the motor, the rotating shaft, the tooth-lacking gear, the rack and the supporting plate, the manual operation is replaced, the time and the labor are saved, and meanwhile, the cracking effect of the exosome is ensured.

Description

Exosome separator

Technical Field

One or more embodiments of the present description relate to the field of exosome-separating devices, in particular to an exosome-separating device.

Background

Exosomes refer to small membrane vesicles (30-150nm) containing complex RNAs and proteins, which today refer specifically to discoidal vesicles with diameters between 40-100 nm. In 1983, exosomes were first found in sheep reticulocytes, which was named "exosomes" by Johnstone in 1987. Many cells secrete exosomes under both normal and pathological conditions. It is mainly from the multivesicular body formed by the invagination of intracellular lysosome particles, and is released into extracellular matrix after the fusion of the outer membrane of the multivesicular body and cell membrane.

In the prior art, few reagents are available on the market for extracting exosome RNA, and the RNA precipitation method is the most commonly used method at present. In the method, in the process of extracting the RNA of the exosome, the exosome and the exosome need to be uniformly mixed firstly, and then the test tube is kept still for precipitation, but the exosome is difficult to be fully mixed with the reagent due to the extremely viscous property, the exosome needs manual vibration, the time and the labor are wasted, and if the vibration is insufficient, the problems of long time for cracking the exosome, extremely difficult full cracking and the like can be caused.

SUMMERY OF THE UTILITY MODEL

In view of the above, it is an object of one or more embodiments of the present disclosure to provide an exosome separation device to solve the problems set forth in the background art.

Based on above-mentioned purpose, one or more embodiments of this specification provide an exosome separator, include the separator box, place the case and vibrate the mechanism, the separator box side is equipped with the chamber door, it is equipped with the end door to place the case side, and places the case and be located inside the separator box, it is used for making and places the case vibrations of reciprocating from top to bottom in the separator box is inside to vibrate the mechanism, it includes motor, pivot, scarce tooth gear, rack and backup pad to vibrate the mechanism, the motor sets up in the separator box side, the pivot is rotated and is connected the interior bottom at the separator box, lack the tooth gear setting in the pivot, rack sliding connection is inside the separator box, and rack and scarce tooth gear intermittent type nature mesh mutually, the backup pad sets up at the rack top, it is located the backup pad to place the case.

Preferably, vibrate the mechanism and still include the limiting plate, the limiting plate sets up inside the separator box, and is used for spacing for placing the seat.

Preferably, the oscillating mechanism further comprises a spring, the spring is connected to the inner bottom of the separating box, and the spring is tightly connected with the rack.

Preferably, still include carousel, driving machine and telescopic link, the carousel rotates to be connected on placing the seat, and places the seat and set up on the carousel, the driving machine sets up at the separation box top, the well kenozooecium of telescopic link rotates to be connected at the interior top of separation box, and is rotated by the driving machine drive, the pars contractilis of telescopic link with place seat fixed connection.

More preferably, still include transmission shaft, first helical gear and second helical gear, the transmission shaft rotates to be connected inside the separator box, and the transmission shaft passes through the drive belt and is connected with the well kenozooecium transmission of telescopic link, first helical gear sets up on the transmission shaft, the second helical gear sets up in the pivot, and first helical gear meshes with the second helical gear mutually.

From the above, it can be seen that the beneficial effects of the present invention are: according to the reciprocating type extracorporeal body treatment device, the motor, the rotating shaft, the gear with missing teeth, the rack and the supporting plate can drive the placing box to move upwards firstly and then move downwards to reciprocate, so that the vibration operation of the placing box is realized, manual operation is replaced, time and labor are saved, and meanwhile, the cracking effect of an exosome is ensured; according to the utility model, the rotating disc, the driving machine and the telescopic rod can drive the box to prevent rotation while the box is placed to vibrate, so that the cracking effect of exosomes is further improved; according to the utility model, the transmission shaft, the first bevel gear and the second bevel gear replace the operation of a driving machine, and the driving source is used for driving the placing box to rotate while vibrating, so that resources are saved.

Drawings

In order to more clearly illustrate one or more embodiments or prior art solutions of the present specification, the drawings that are needed in the description of the embodiments or prior art will be briefly described below, and it is obvious that the drawings in the following description are only one or more embodiments of the present specification, and that other drawings may be obtained by those skilled in the art without inventive effort from these drawings.

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural diagram of an oscillating mechanism according to the present invention;

FIG. 3 is a schematic structural view of a preferred embodiment of the present invention;

FIG. 4 is a schematic structural diagram of another preferred embodiment of the present invention.

In the reference symbols: 1. a separation tank; 2. a vibrating mechanism; 3. placing a box; 4. a driver; 5. a telescopic rod; 6, driving the belt; 7. a drive shaft; 8. a first helical gear; 9. a second helical gear; 21. a turntable; 22. a support plate; an electric motor; 24. a gear with missing teeth; 25. a rack; 26. a rotating shaft.

Detailed Description

To make the objects, technical solutions and advantages of the present disclosure more apparent, the present disclosure is further described in detail below with reference to specific embodiments.

It is to be noted that unless otherwise defined, technical or scientific terms used in one or more embodiments of the present specification should have the ordinary meaning as understood by those of ordinary skill in the art to which this disclosure belongs. The use of "first," "second," and similar terms in one or more embodiments of the specification is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.

Example one

Referring to fig. 1-3, an exosome separating device comprises a separating box 1, a placing box 3 and a vibrating mechanism 2, wherein a box door is arranged on the side surface of the separating box 1, an end door is arranged on the side surface of the placing box 3, the placing box 3 is positioned in the separating box 1, the oscillating mechanism 2 is used for enabling the placing box 3 to oscillate up and down in the separating box 1, the oscillating mechanism 2 comprises a motor 23, a rotating shaft 26, a gear with missing teeth 24, a rack 25 and a supporting plate 22, the motor 23 is arranged on the side surface of the separation box 1, the rotating shaft 26 is rotatably connected to the inner bottom of the separation box 1, the gear with missing teeth 24 is arranged on a rotating shaft 26, the rack 25 is connected in a sliding way inside the separating box 1, and rack 25 meshes with the gear of lacking teeth 24 intermittent type nature mutually, backup pad 22 sets up at rack 25 top, it is located backup pad 22 to place case 3. According to the utility model, the motor 23, the rotating shaft 26, the gear with missing teeth 24, the rack 25 and the supporting plate 22 can drive the placing box 3 to move up and down in a reciprocating motion, so that the vibration operation of the placing box 3 is realized, manual operation is replaced, time and labor are saved, and meanwhile, the cracking effect of exosomes is ensured.

As the improvement scheme of above-mentioned scheme, vibrate mechanism 2 still includes the limiting plate, the limiting plate sets up inside separator box 1, and is used for carrying on spacingly for placing the seat.

As a modification of the above solution, the oscillating mechanism 2 further includes a spring, the spring is connected to the inner bottom of the separating box 1, and the spring is tightly connected to the rack 25.

As an improvement of the scheme, the disposable exosome splitting device further comprises a rotary table 21, a driving machine 4 and an expansion link 5, wherein the rotary table 21 is rotatably connected to a placing seat, the placing seat is arranged on the rotary table 21, the driving machine 4 is arranged at the top of the separation box 1, a hollow part of the expansion link 5 is rotatably connected to the inner top of the separation box 1 and is driven by the driving machine 4 to rotate, and a telescopic part of the expansion link 5 is fixedly connected with the placing seat.

The working process comprises the following steps: the test tube that will be equipped with the exosome is placed inside placing case 3, and operation motor 23, motor 23's output rotate and drive and lack tooth gear 24 and rotate, lack tooth gear 24 and rack 25 intermittent type nature meshing motion, reciprocating motion about rack 25, and then drive backup pad 22 reciprocating motion from top to bottom, and then drive and place case 3 reciprocating motion from top to bottom, realize placing the vibration operation of case 3.

When placing case 3 vibrations, operation driving machine 4, the output of driving machine 4 rotates and drives telescopic link 5 and rotate, and telescopic link 5 drives and places case 3 and rotate through carousel 21.

Example two

Referring to fig. 4, as an improvement of the above scheme, the present invention further includes a transmission shaft 7, a first bevel gear 8 and a second bevel gear 9, the transmission shaft 7 is rotatably connected inside the separation box 1, the transmission shaft 7 is in transmission connection with the hollow portion of the telescopic rod 5 through a transmission belt 6, the first bevel gear 8 is disposed on the transmission shaft 7, the second bevel gear 9 is disposed on the rotation shaft 26, and the first bevel gear 8 is engaged with the second bevel gear 9, the present invention replaces the operation of the driving machine 4 with the transmission shaft 7, the first bevel gear 8 and the second bevel gear 9, and uses one driving source to drive the placing box 3 to rotate while oscillating, so as to save resources.

The working process comprises the following steps: the pivot 26 rotates and drives second helical gear 9 and rotates, and second helical gear 9 rotates with the meshing of first helical gear 8, and first helical gear 8 drives transmission shaft 7 and rotates, and transmission shaft 7 passes through drive belt 6 and drives telescopic link 5 and rotate, and telescopic link 5 drives and places case 3 and rotate through carousel 21.

It is intended that the one or more embodiments of the present specification embrace all such alternatives, modifications and variations as fall within the broad scope of the appended claims. Therefore, any omissions, modifications, substitutions, improvements, and the like that may be made without departing from the spirit and principles of one or more embodiments of the present disclosure are intended to be included within the scope of the present disclosure.

Claims (5)

1. An exosome-separating device, comprising:

the separation box (1), wherein a box door is arranged on the side surface of the separation box (1);

the side surface of the placing box (3) is provided with an end door, and the placing box (3) is positioned inside the separating box (1); and

vibrate mechanism (2), vibrate mechanism (2) and be used for making and place case (3) reciprocal vibrations about separator box (1) is inside, vibrate mechanism (2) and include:

the motor (23), the said motor (23) is set up in the side of the separation box (1);

the rotating shaft (26), the rotating shaft (26) is rotatably connected to the inner bottom of the separating box (1);

the gear (24) is arranged on the rotating shaft (26);

the rack (25) is connected inside the separating box (1) in a sliding manner, and the rack (25) is intermittently meshed with the gear lacking gear (24); and

the supporting plate (22), the supporting plate (22) is arranged on the top of the rack (25), and the placing box (3) is located on the supporting plate (22).

2. An exosome separating device according to claim 1, characterized in that the oscillation mechanism (2) further comprises a limiting plate arranged inside the separating tank (1) and used to limit the placing tank.

3. An exosome separating device according to claim 1, characterized in that the oscillating mechanism (2) further comprises a spring, which is connected to the inner bottom of the separating chamber (1) and which is tightly connected to the rack (25).

4. An exosome-separating device according to claim 1, further comprising:

the rotary table (21), the rotary table (21) is rotatably connected to the placing box, and the placing box is arranged on the rotary table (21);

the driving machine (4), the driving machine (4) is arranged at the top of the separation box (1); and

the hollow part of the telescopic rod (5) is rotatably connected to the inner top of the separation box (1) and is driven to rotate by the driving machine (4), and the telescopic part of the telescopic rod (5) is fixedly connected with the placing box.

5. An exosome-separating device according to claim 4, further comprising:

the transmission shaft (7) is rotatably connected inside the separation box (1), and the transmission shaft (7) is in transmission connection with the hollow part of the telescopic rod (5) through a transmission belt (6);

the first bevel gear (8), the said first bevel gear (8) is set up on the drive shaft (7); and

the second bevel gear (9), the second bevel gear (9) is arranged on the rotating shaft (26), and the first bevel gear (8) is meshed with the second bevel gear (9).

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