CN216614478U - Monoclonal antibody chromatography pressure control device - Google Patents

Abstract

The utility model discloses a monoclonal antibody chromatography pressure control device, comprising: the device comprises a chromatography device and a control component arranged in the chromatography device, wherein the chromatography device comprises a chromatography column with a hollow cylinder structure and a liquid inlet pipe connected with the chromatography column, a dialysis layer and a seepage port are arranged at the bottom of the chromatography column, and an overflow port is arranged at the top of the chromatography column; the liquid inlet pipe is connected with the dialysis layer and is positioned on one side of the dialysis layer away from the seepage port; the liquid inlet pipe is provided with a pump body; the control assembly is arranged between the dialysis layer and the overflow outlet and comprises a sterilization ultraviolet light source and a floating tank, the sterilization ultraviolet light source is fixedly connected in the floating tank body, the internal pressure of the chromatographic column can be monitored through the floating state of the floating tank, and the light source not only can carry out deep immersion sterilization, but also can improve the observation effect of the position of the floating tank.

Description

Monoclonal antibody chromatography pressure control device

Technical Field

The utility model relates to the field of monoclonal antibody preparation, in particular to a monoclonal antibody chromatography pressure control device.

Background

A highly homogeneous antibody, called monoclonal antibody, generated by a single B cell clone and directed only to a specific epitope is generally prepared by a hybridoma technique, wherein the hybridoma (hybridoma) antibody technique is that on the basis of a cell fusion technique, sensitized B cells with the capacity of secreting specific antibodies and myeloma cells with the capacity of proliferation are fused into B cell hybridomas, a single hybridoma cell with the characteristics is cultured into a cell group to prepare a specific antibody, namely a monoclonal antibody, directed to one epitope, specifically, a monoclonal antibody is required to be prepared, namely, a monoclonal B lymphocyte capable of synthesizing the antibody is obtained firstly, but the B lymphocyte cannot grow in vitro, experiments show that the myeloma cells can grow in vitro, and the myeloma cells and the immune lymphocytes are combined into a whole by applying a cell hybridization technique, obtaining a hybrid myeloma cell which inherits the characteristics of two parent cells and has both the characteristics of B lymphocyte to synthesize antibody and the characteristics of myeloma cell capable of being cultured and proliferated in vitro, and using the cell group obtained by culturing and proliferating the single fused cell, a monoclonal antibody against an antigenic determinant can be prepared.

In the preparation of monoclonal antibody is applied to affinity chromatography in-process, set up ultraviolet source in liquid inlet and liquid outlet position and disinfect usually, do not have a device that can carry out immersive sterilization to chromatography column inside yet, and to hydraulic control in the chromatography column, different antibody solution correspond has the best chromatography pressure of difference, only through the manometer on the outside pipeline, the operator is difficult to handle the best purification efficiency of accuse, do not have one kind at present and make things convenient for direct monitoring internal pressure promptly, can provide the integrated device of high-efficient sterilization again.

Disclosure of Invention

The utility model overcomes the defects of the prior art and provides a monoclonal antibody chromatography pressure control device.

In order to achieve the purpose, the utility model adopts the technical scheme that: a monoclonal antibody chromatography pressure control device comprising: chromatography device and set up the control assembly in the chromatography device characterized in that: the chromatography device comprises a chromatography column with a hollow cylinder structure and a liquid inlet pipe connected with the chromatography column, a dialysis layer and a seepage port are arranged at the bottom of the chromatography column, and an overflow port is arranged at the top of the chromatography column; the liquid inlet pipe is connected with the dialysis layer and is positioned on one side of the dialysis layer away from the seepage port; the liquid inlet pipe is provided with a pump body; the control assembly sets up the dialysis layer with overflow mouth intermediate position, the control assembly includes sterilization ultraviolet source and floats the groove, sterilization ultraviolet source fixed connection float the inslot in the groove, float the groove opening orientation chromatography column top.

In a preferred embodiment of the utility model, a limiting rope is connected to the floating groove, and the other end of the limiting rope is connected to the top of the chromatographic device.

In a preferred embodiment of the utility model, when the limiting rope is enlarged to the maximum length, the height of the floating groove is greater than the connecting position of the liquid inlet pipe and the chromatographic column.

In a preferred embodiment of the present invention, a check valve is disposed at the position of the overflow outlet.

In a preferred embodiment of the utility model, the seepage port of the chromatographic column is communicated with an external heat-preservation storage device.

In a preferred embodiment of the utility model, the wall of the floating tank is made of transparent material.

In a preferred embodiment of the utility model, a pressure sensor is arranged on the inner side of the top of the chromatographic column.

In a preferred embodiment of the utility model, the top of the chromatographic column is detachably connected with the side wall of the chromatographic column.

In a preferred embodiment of the utility model, the top and the side wall of the chromatographic column are rotatably connected through threads.

In a preferred embodiment of the utility model, the diameter of the upper opening of the floating groove is larger than that of the overflow outlet.

The utility model solves the defects in the background art, and has the following beneficial effects:

(1) in the utility model, the floating trough is arranged and can be always kept above the liquid level, thereby monitoring the internal pressure through the floating state, particularly, after the liquid inlet pipe is opened to start purification, the floating groove rises along with the liquid level until contacting the top of the chromatographic column, the pump body of the liquid inlet pipe continuously applies pressure, after the floating groove is filled with the solution, slowly descends under the gravity condition, if the floating groove does not descend to the limit rope to be completely straightened, the solution overflowing from the top overflow port for reducing pressure is excessive, and the excessive antibody is easily discharged by mistake, at the moment, the pressure of the pump body needs to be reduced, if the floating groove descends to the limit rope to be completely straightened, it is indicated that the dialysis state is normal by only maintaining the solution filled in the column and the operator can maintain the critical point between the height and the rise through the floating tank at the lowest position, thereby obtaining the optimal chromatography pressure corresponding to the antibody solution.

(2) The floating tank is made of non-transparent materials, so that the ultraviolet light source can move in the solution in the floating moving process of the floating tank, and the optimal sterilization effect is achieved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments described in the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts;

FIG. 1 is a schematic plan view of a preferred embodiment of the present invention;

in the figure: 1. a chromatography column; 2. a liquid inlet pipe; 3. a pump body; 4. an overflow outlet; 5. a percolation outlet; 6. a dialysis layer; 7. a floating trough; 8. a limiting rope; 9. a one-way valve.

Detailed Description

The present invention will now be described in further detail with reference to the accompanying drawings, which are simplified schematic drawings illustrating only the basic structure of the utility model and thus showing only the constructions relating to the utility model, and also with reference to "an embodiment", "one embodiment" or "another embodiment" in the description of the utility model indicating that a particular feature, structure or characteristic described in connection with the embodiment is included in at least some embodiments, but not necessarily all embodiments.

As shown in fig. 1, a monoclonal antibody chromatography pressure control device includes: the chromatography device comprises a chromatography column 1 with a hollow cylindrical structure and a liquid inlet pipe 2 connected with the chromatography column 1, wherein the bottom of the chromatography column 1 is provided with a dialysis layer 6 and a seepage port 5, and the top of the chromatography column 1 is provided with an overflow port 4; the liquid inlet pipe 2 is connected with the dialysis layer 6 and is positioned on one side of the dialysis layer 6 far away from the seepage port 5; the liquid inlet pipe 2 is provided with a pump body 3; control assembly sets up at dialysis layer 6 and 4 intermediate positions of overflow mouth, control assembly is including sterilization ultraviolet source and float groove 7, sterilization ultraviolet source fixed connection is internal at float groove 7 groove, float 7 openings in the groove towards chromatographic column 1 top, it is connected spacing rope 8 to float on the groove 7, the chromatographic device top is connected to the spacing rope 8 other end, it is preferred, spacing rope 8 enlargies when to the maximum length, it highly is greater than inlet tube 2 and 1 hookup location of chromatographic column to float the groove 7, the solution that inlet tube 2 was irritated under initial condition can directly not get into and float 7 inslots, make it slowly fall after can rising to the top smoothly, make pressure observation more obvious convenient.

Preferably, the overflow outlet 4 is provided with a one-way valve 9, which can prevent backflow and influence the floating state of the floating tank 7.

Preferably, the seepage port 5 of the chromatographic column 1 is communicated with an external heat-preservation storage device and can be stored for a subsequent detection link.

In a preferred embodiment, a pressure sensor is disposed on the inner side of the top of the chromatographic column 1, so as to detect a liquid hammer phenomenon generated when the pump body 3 adjusts the pressure in time, thereby prompting an operator to adjust the pressure slowly.

Preferably, the connection can be dismantled in the chromatographic column 1 top rather than its lateral wall screw thread rotation, need explain that, in single purification process, solution is at the beginning irritate the back, and solution remains throughout to be full of the state of chromatographic column 1 among the continuous purification process, consequently need not to clear up solution in the floating tank 7, and when preparing purification next time, can be convenient open chromatographic column 1, will float the original antibody solution clearance in the tank 7, avoid with the follow-up antibody solution doping that goes on purifying.

Preferably, the diameter of the upper opening of the floating tank 7 is larger than that of the overflow outlet 4, so that the floating tank 7 can be prevented from influencing pressure monitoring through the overflow outlet 4.

In the utility model, the floating tank 7 is arranged, and the floating tank 7 can be always kept above the liquid level, so that the internal pressure is monitored through the floating state, specifically, after the liquid inlet pipe 2 is opened to start purification, the floating tank 7 rises along with the liquid level until contacting the top of the chromatographic column 1, the pump body 3 of the liquid inlet pipe 2 continues to apply pressure, after the floating tank 7 is filled with the solution, slowly descends under the gravity condition, if the floating tank 7 does not descend to the limit rope 8 and is completely straightened, the situation shows that the solution overflowing from the top overflow port 4 for reducing pressure is too much, and the excessive antibody is easily discharged by mistake, at the moment, the pressure of the pump body 3 needs to be reduced, if the floating tank 7 descends to the limit rope 8 and is completely straightened, it is indicated that the dialysis state is normal by only maintaining the solution filled in the chromatography column 1, and the operator can maintain the critical point between the height and the rise through the floating tank 7 at the lowest position, thereby obtaining the optimum chromatography pressure corresponding to the antibody solution.

In the utility model, the floating tank 7 is made of non-transparent materials, so that the ultraviolet light source can move in the solution in the floating moving process of the floating tank 7, thereby obtaining the optimal sterilization effect.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

In light of the foregoing description of the preferred embodiments of the present invention, it is to be understood that various changes and modifications may be made by those skilled in the art without departing from the spirit and scope of the utility model. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims (10)

1. A monoclonal antibody chromatography pressure control device comprising: chromatography device and set up the control assembly in the chromatography device characterized in that:

the chromatography device comprises a chromatography column with a hollow cylinder structure and a liquid inlet pipe connected with the chromatography column, a dialysis layer and a seepage port are arranged at the bottom of the chromatography column, and an overflow port is arranged at the top of the chromatography column; the liquid inlet pipe is connected with the dialysis layer and is positioned on one side of the dialysis layer away from the seepage port; the liquid inlet pipe is provided with a pump body;

the control assembly sets up the dialysis layer with overflow mouth intermediate position, the control assembly includes sterilization ultraviolet source and floats the groove, sterilization ultraviolet source fixed connection float the inslot in the groove, float the groove opening orientation chromatography column top.

2. The monoclonal antibody chromatography pressure control device according to claim 1, wherein: the floating groove is connected with a limiting rope, and the other end of the limiting rope is connected with the top of the chromatography device.

3. The monoclonal antibody chromatography pressure control device according to claim 2, wherein: when the limiting rope is enlarged to the maximum length, the height of the floating groove is larger than the connecting position of the liquid inlet pipe and the chromatographic column.

4. The monoclonal antibody chromatography pressure control device according to claim 1, wherein: and a one-way valve is arranged at the overflow outlet.

5. The monoclonal antibody chromatography pressure control device according to claim 1, wherein: and the seepage port of the chromatographic column is communicated with an external heat-preservation storage device.

6. The monoclonal antibody chromatography pressure control device according to claim 1, wherein: the wall of the floating tank is made of transparent material.

7. The monoclonal antibody chromatography pressure control device according to claim 1, wherein: and a pressure sensor is arranged on the inner side of the top of the chromatographic column.

8. The monoclonal antibody chromatography pressure control device according to claim 1, wherein: the top of the chromatographic column is detachably connected with the side wall of the chromatographic column.

9. The monoclonal antibody chromatography pressure control device according to claim 8, wherein: the top of the chromatographic column is rotationally connected with the side wall through threads.

10. The monoclonal antibody chromatography pressure control device according to claim 1, wherein: the diameter of the upper opening of the floating groove is larger than that of the overflow outlet.

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