CN216614477U - A purify device fast for monoclonal antibody - Google Patents
A purify device fast for monoclonal antibody Download PDFInfo
- Publication number
- CN216614477U CN216614477U CN202122973109.1U CN202122973109U CN216614477U CN 216614477 U CN216614477 U CN 216614477U CN 202122973109 U CN202122973109 U CN 202122973109U CN 216614477 U CN216614477 U CN 216614477U
- Authority
- CN
- China
- Prior art keywords
- column
- monoclonal antibody
- analysis column
- purification device
- rapid purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The utility model discloses a rapid purification device for monoclonal antibodies, which comprises: the chromatography component comprises a primary analysis column, a secondary analysis column and a secondary analysis column, the top of the chromatography component is provided with a discharge port, the bottom of the chromatography component is provided with a main body structure of a dialysis layer and a seepage port, the middle section of the length path of the primary analysis column is communicated with an introduction pipe for introducing antibody protein liquid, the introduction pipe is provided with a pressure pump, the middle section of the length path of the secondary analysis column is provided with a secondary analysis inlet, and the secondary analysis inlet is communicated with the discharge port of the primary analysis column; the middle section of the length path of the secondary analytical column is provided with two inlets which are respectively connected with the seepage port of the reanalysis column and the seepage port of the primary analytical column, and the inlet is provided with a flow balance mechanism.
Description
Technical Field
The utility model relates to the field of monoclonal antibody preparation, in particular to a rapid purification device for a monoclonal antibody.
Background
A highly homogeneous antibody, called monoclonal antibody, generated by a single B cell clone and directed only to a specific epitope is generally prepared by a hybridoma technique, wherein the hybridoma (hybridoma) antibody technique is that on the basis of a cell fusion technique, sensitized B cells with the capacity of secreting specific antibodies and myeloma cells with the capacity of proliferation are fused into B cell hybridomas, a single hybridoma cell with the characteristics is cultured into a cell group to prepare a specific antibody, namely a monoclonal antibody, directed to one epitope, specifically, a monoclonal antibody is required to be prepared, namely, a monoclonal B lymphocyte capable of synthesizing the antibody is obtained firstly, but the B lymphocyte cannot grow in vitro, experiments show that the myeloma cells can grow in vitro, and the myeloma cells and the immune lymphocytes are combined into a whole by applying a cell hybridization technique, obtaining a hybrid myeloma cell which inherits the characteristics of both of the two parental cells and has the characteristics of producing antibodies by B lymphocytes and the characteristics of proliferating myeloma cells in vitro culture, and preparing a monoclonal antibody against an antigenic determinant using a cell population derived from the cultured proliferation of a single fused cell.
In the process of applying the preparation of the monoclonal antibody to affinity chromatography, the currently adopted single pressurization chromatography method is difficult to accurately control the pressure, and most operators still manually pressurize the antibody chromatography, and in the manual operation process, no clear layering exists, so that the waste of partial antibodies is often caused in the separation process, and a device which can repeatedly dialyze the discharged waste liquid and rapidly realize multiple times of percolation is not provided.
Disclosure of Invention
The utility model overcomes the defects of the prior art and provides a device for quickly purifying the monoclonal antibody.
In order to achieve the purpose, the utility model adopts the technical scheme that: a rapid purification apparatus for monoclonal antibodies, comprising: chromatography subassembly and setting are in the flow balance mechanism in the chromatography subassembly, its characterized in that: the chromatography component comprises a primary analysis column, a secondary analysis column and a secondary analysis column, wherein the top of the primary analysis column, the bottom of the secondary analysis column and the secondary analysis column are respectively provided with a main body structure with a discharge port, a dialysis layer and a seepage port, the middle section of the length path of the primary analysis column is communicated with an introduction pipe for introducing antibody protein liquid, the introduction pipe is provided with a pressure pump, the middle section of the length path of the secondary analysis column is provided with a secondary analysis inlet, and the secondary analysis inlet is communicated with the discharge port of the primary analysis column; two inlets are arranged at the middle section of the length path of the secondary analysis column and are respectively connected with the seepage port of the secondary analysis column and the seepage port of the primary analysis column, and a flow balance mechanism is arranged at the inlet.
In a preferred embodiment of the utility model, the two inlets on the secondary chromatography column are of the same height.
In a preferred embodiment of the present invention, the flow balancing mechanism includes a flow detector disposed adjacent to the access port.
In a preferred embodiment of the present invention, the flow detectors are disposed on an inner wall of the access port, and the flow detectors are disposed in one-to-one correspondence with the access ports.
In a preferred embodiment of the present invention, the flow balancing mechanism is electrically connected to the pressure pump.
In a preferred embodiment of the present invention, the communicating tubes between the first analytical column, the second analytical column and the secondary analytical column are all made of flexible material tubes.
In a preferred embodiment of the utility model, each outlet position in the chromatography module is provided with a one-way valve.
In a preferred embodiment of the present invention, the two side inlets of the secondary analytical column are respectively provided with a sterilizing lamp.
In a preferred embodiment of the present invention, the permeation port of the secondary analytical column is connected to an external thermal insulation storage device.
In a preferred embodiment of the utility model, a sterilizing lamp is arranged at the connecting position of the introducing pipe and the chromatography column.
The utility model solves the defects in the background technology, and has the following beneficial effects:
the utility model can collect the waste liquid discharged excessively and then dialyze again, thus avoiding the antibody from being discharged by mistake due to overlarge pressure in the primary column, ensuring the residual after dialysis and improving the yield after purification.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments described in the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts;
FIG. 1 is a schematic plan view of a preferred embodiment of the present invention;
in the figure: 1. a pressure pump; 2. an introducing pipe; 3. first separating out the column; 4. a re-analytical column; 5. secondary column chromatography; 6. a dialysis layer; 7. a heat preservation storage device; 8. a one-way valve.
Detailed Description
The present invention will now be described in further detail with reference to the accompanying drawings, which are simplified schematic drawings illustrating only the basic structure of the utility model and thus showing only the constructions relating to the utility model, and also with reference to "an embodiment", "one embodiment" or "another embodiment" in the description of the utility model indicating that a particular feature, structure or characteristic described in connection with the embodiment is included in at least some embodiments, but not necessarily all embodiments.
As shown in fig. 1, a rapid purification apparatus for monoclonal antibodies, comprising: the chromatography component comprises a primary analysis column 3, a secondary analysis column 4 and a secondary analysis column 5, the top of the chromatography component, the bottom of the chromatography component and the secondary analysis column are provided with a discharge port, the dialysis layer 6 and a main body structure of a seepage port are arranged at the bottom of the chromatography component, the dialysis layer 6 preferably adopts molecular sieve chromatography, the middle section of the length path of the primary analysis column 3 is communicated with an introduction pipe 2 for introducing antibody protein liquid, the introduction pipe 2 is provided with a pressure pump 1, the middle section of the length path of the secondary analysis column 4 is provided with a secondary analysis inlet, and the secondary analysis inlet is communicated with the discharge port of the primary analysis column 3; the 5 length route middle section positions of secondary analysis post are provided with two and lead to the entry, and two lead to the entry highly uniform, thereby make from the solution that secondary analysis post 4 and first analysis post 3 let in can the intensive mixing, thereby make and realize better purification effect in the secondary analysis post 5, connect the infiltration mouth of secondary analysis post 4 and the infiltration mouth of first analysis post 3 respectively, the access mouth position is provided with flow balance mechanism, discharge port position in the chromatography subassembly all is provided with check valve 8, avoid the backward flow, the infiltration exit linkage outside heat preservation storage device 7 of secondary analysis post 5.
In an embodiment, the flow balance mechanism includes a flow detector disposed near the inlet, the flow detector is disposed on the inner wall of the inlet, the flow detector and the inlet are disposed in a one-to-one correspondence manner, the flow balance mechanism is electrically connected to the pressure pump 1, when the flow from one side of the chromatographic column 4 is greater than the flow from one side of the chromatographic column 3, it indicates that the pressure in the chromatographic column 3 is too large, the pressure pump 1 reduces the output pressure thereof, and when the flow from one side of the chromatographic column 4 is less than the flow from one side of the chromatographic column 3, it indicates that the pressure in the chromatographic column 3 is insufficient, the overall filtering capability of the device is not saturated, and the output capability of the pressure pump 1 is increased.
Preferably, the communicating pipes among the primary analytical column 3, the secondary analytical column 4 and the secondary analytical column 5 are all made of flexible material pipes, so that the pipelines can bear the phenomenon of liquid hammer, and the dialysis layer 6 is prevented from being damaged.
Preferably, the two side inlets of the secondary chromatography column 5 are respectively provided with a sterilizing lamp, and the connecting position of the introducing pipe 2 and the primary chromatography column 3 is provided with a sterilizing lamp, so that the sterilization can be realized in the whole chromatography process.
The utility model can collect the waste liquid discharged excessively and then dialyze again, thus avoiding the antibody from being discharged by mistake due to overlarge pressure in the primary chromatographic column 3, ensuring the residual after dialysis and improving the yield after purification, and can carry out secondary dialysis after converging the protein liquid seeped out of the primary chromatographic column 3 and the protein liquid seeped out of the secondary chromatographic column 4, thereby ensuring the purification effect.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
In light of the foregoing description of the preferred embodiments of the present invention, it is to be understood that various changes and modifications may be made by those skilled in the art without departing from the spirit and scope of the utility model. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (10)
1. A rapid purification apparatus for monoclonal antibodies, comprising: chromatography subassembly and setting are in the flow balance mechanism in the chromatography subassembly, its characterized in that:
the chromatography component comprises a primary analysis column, a secondary analysis column and a secondary analysis column, wherein the top of the primary analysis column, the bottom of the secondary analysis column and the secondary analysis column are respectively provided with a main body structure with a discharge port, a dialysis layer and a seepage port, the middle section of the length path of the primary analysis column is communicated with an introduction pipe for introducing antibody protein liquid, the introduction pipe is provided with a pressure pump, the middle section of the length path of the secondary analysis column is provided with a secondary analysis inlet, and the secondary analysis inlet is communicated with the discharge port of the primary analysis column;
two inlets are arranged at the middle section of the length path of the secondary analysis column and are respectively connected with the seepage port of the secondary analysis column and the seepage port of the primary analysis column, and a flow balance mechanism is arranged at the inlet.
2. The rapid purification device for monoclonal antibody according to claim 1, wherein: the two inlets on the secondary chromatographic column are identical in height.
3. The rapid purification device for monoclonal antibody according to claim 2, wherein: the flow balancing mechanism includes a flow detector disposed proximate the access port.
4. The rapid purification device for monoclonal antibody according to claim 3, wherein: the flow detector is arranged on the inner wall of the access port, and the flow detector and the access port are arranged in a one-to-one correspondence mode.
5. The rapid purification device for monoclonal antibody according to claim 1, wherein: the flow balance mechanism is electrically connected with the pressure pump.
6. The rapid purification device for monoclonal antibody according to claim 1, wherein: the first analytical column, the second analytical column and the communicating pipe between the secondary analytical columns are all made of flexible material pipes.
7. The rapid purification device for monoclonal antibody according to claim 6, wherein: and a one-way valve is arranged at each discharge port in the chromatography component.
8. The rapid purification device for monoclonal antibody according to claim 1, wherein: and sterilizing lamps are respectively arranged at the positions of the inlet openings at the two sides of the secondary chromatographic column.
9. The rapid purification device for monoclonal antibody according to claim 1, wherein: and the seepage port of the secondary analytical column is connected with an external heat-preservation storage device.
10. The rapid purification device for monoclonal antibody according to claim 1, wherein: and a sterilizing lamp is arranged at the connecting position of the introducing pipe and the primary chromatographic column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122973109.1U CN216614477U (en) | 2021-11-30 | 2021-11-30 | A purify device fast for monoclonal antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122973109.1U CN216614477U (en) | 2021-11-30 | 2021-11-30 | A purify device fast for monoclonal antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216614477U true CN216614477U (en) | 2022-05-27 |
Family
ID=81700403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122973109.1U Active CN216614477U (en) | 2021-11-30 | 2021-11-30 | A purify device fast for monoclonal antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216614477U (en) |
-
2021
- 2021-11-30 CN CN202122973109.1U patent/CN216614477U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0730639B1 (en) | Cell separation device | |
| JP2002537087A (en) | Apparatus for concentration and / or purification of macromolecules in solution and method for producing such an apparatus | |
| TW201509432A (en) | Matrix of affinity chromatography | |
| CN216614477U (en) | A purify device fast for monoclonal antibody | |
| CN106365306A (en) | Membrane component used for water treatment | |
| CN111220800A (en) | Exosome detection chip based on micro-fluidic droplet technology | |
| CN1836616A (en) | Packed water drinking machine | |
| CN110653012A (en) | Multistage microfluidic chip device for sorting, enriching and detecting circulating tumor cells | |
| CN104020012A (en) | Sampling filtering bag for cryptospsridium and giardia detection in water | |
| CN211676417U (en) | Chromatographic column and combined chromatographic column | |
| CN206692494U (en) | It is a kind of for the external air of ecological tank and the device of water filtration | |
| CN105879687A (en) | Ceramic hollow fiber membrane filter element and assembly thereof | |
| CN110272487A (en) | A kind of pipette tips formula dispersed solid phase microextraction column, the purification process based on the pipette tips formula dispersed solid phase microextraction column and application | |
| CN111172009A (en) | Integrated exosome separation chip and preparation method and application thereof | |
| CN110358676A (en) | The microlayer model sampler applied in the detection of digital pcr imaging method | |
| CN206996294U (en) | A kind of element kit and water purifier | |
| CN102728088A (en) | Outer circulation vacuum rectification tower | |
| CN116042373A (en) | System for continuous production and separation and purification of extracellular body and use method thereof | |
| CN110465335A (en) | A kind of micro-fluidic chip and fixture | |
| CN116121031A (en) | Multistage microfluidic chip for single cell screening and preparation method thereof | |
| CN209968132U (en) | Filter membrane structure for whole blood filtration | |
| CN219897676U (en) | Glycopeptide enrichment device | |
| CN220294166U (en) | Antibody purification device | |
| CN109529444A (en) | The filtering technique and filter of haemocyte in a kind of removal blood sample | |
| CN206229210U (en) | Twin-stage Hollow fiber units vaccine concentrator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |