CN216594694U - Bacterium counting assembly with sheath flow impedance sensor - Google Patents
Bacterium counting assembly with sheath flow impedance sensor Download PDFInfo
- Publication number
- CN216594694U CN216594694U CN202121837963.9U CN202121837963U CN216594694U CN 216594694 U CN216594694 U CN 216594694U CN 202121837963 U CN202121837963 U CN 202121837963U CN 216594694 U CN216594694 U CN 216594694U
- Authority
- CN
- China
- Prior art keywords
- bacteria
- hole
- cell
- liquid
- counting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 174
- 239000007788 liquid Substances 0.000 claims abstract description 118
- 210000004027 cell Anatomy 0.000 claims abstract description 100
- 238000002955 isolation Methods 0.000 claims abstract description 45
- 230000009471 action Effects 0.000 claims abstract description 11
- 210000003850 cellular structure Anatomy 0.000 claims abstract description 4
- 239000010437 gem Substances 0.000 claims description 142
- 229910001751 gemstone Inorganic materials 0.000 claims description 142
- 230000001580 bacterial effect Effects 0.000 claims description 20
- 238000005070 sampling Methods 0.000 claims description 17
- 238000012545 processing Methods 0.000 claims description 10
- 230000001960 triggered effect Effects 0.000 claims description 5
- 238000005516 engineering process Methods 0.000 abstract description 2
- 230000036619 pore blockages Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 45
- 239000012530 fluid Substances 0.000 description 29
- 238000000034 method Methods 0.000 description 27
- 230000000903 blocking effect Effects 0.000 description 19
- 239000002699 waste material Substances 0.000 description 16
- 230000003750 conditioning effect Effects 0.000 description 14
- 239000002245 particle Substances 0.000 description 14
- 239000000463 material Substances 0.000 description 12
- 239000011148 porous material Substances 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 238000001514 detection method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 238000001914 filtration Methods 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 6
- 230000002159 abnormal effect Effects 0.000 description 5
- 238000010304 firing Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 230000003321 amplification Effects 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 229920006324 polyoxymethylene Polymers 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000010891 electric arc Methods 0.000 description 2
- 238000010892 electric spark Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229920002799 BoPET Polymers 0.000 description 1
- 239000005041 Mylar™ Substances 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- -1 polyoxymethylene Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003245 working effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a bacteria counting device with a sheath flow impedance sensor, comprising: the liquid storage tank, the isolation tank and the counting tank assembly are sequentially connected by pipelines; a device for controlling the flow rate of liquid is connected between the liquid storage tank and the isolation tank; the counting cell component comprises a gem hole, a front cell, a rear cell and electrodes, wherein the front cell and the rear cell are communicated through the gem hole, the electrodes are respectively arranged on two sides of the gem hole, the front cell comprises front sheath liquid, the rear cell comprises rear sheath liquid, and the front sheath liquid enables the bacteria sample to be counted to enter the rear cell from the front cell through the gem hole under the combined action of a positive pressure source of the front cell and a negative pressure source of the rear cell. The introduction of sheath flow technology into the bacteria counting device of the present invention allows bacteria to pass through the gem aperture in a line-up fashion, reducing the risk of pore blockage.
Description
The present application claims priority from the chinese patent application No. 202010791059.2 entitled "a bacteria enumeration apparatus with sheath flow impedance sensor and method thereof," filed on 7/8/2020, which is incorporated herein by reference in its entirety.
Technical Field
The utility model relates to the field of biomedicine, in particular to a bacteria counting device with a sheath flow impedance sensor and a method thereof.
Background
A cytometer is generally an instrument for measuring the number of platelets, white blood cells, red blood cells, and the like. The full-automatic cell counter is widely applied, and the technical scheme of the coulter principle analysis method is an internationally recognized standard control method for measuring the sizes of cells and particles and always occupies an important position in hematology analysis.
The counting device and method applied to bacteria in the prior art also have the following problems: first, no device for measuring the number of bacteria by using a resistance counting method exists in the market at present. Secondly, the aperture of the gem hole of the existing cell counter is suitable for measuring red blood cells, white blood cells and the like with larger sizes, and the cells can be ensured to pass through the gem hole one by one; the bacterium is individual less, and one that can not be normal passes through the precious stone hole, measures the bacterium and probably passes through the number more than or equal to 2 in precious stone hole simultaneously, causes the count inaccurate. Measuring red blood cell, leucocyte etc. if the precious stone hole is less than under 50 um's the condition, can cause the precious stone hole shutoff hole phenomenon of current counting instrument again, so the restriction of prior art precious stone hole aperture is more than 50 um. Fourthly, manual operation: in the prior art, the number of bacteria can be easily and accurately measured by using a microscope, or pictures can be made by a dyeing method, a projection method or a photographing method, and then the calculation is carried out according to the magnification, so that the labor and the material resources are consumed, and the time consumption is large, so that the counting methods are not popularized in a large area in clinical application. Manual operation: the size and shape of the bacteria vary with the species, and the bacteria are branched, filamentous, spindle-shaped, chain-shaped and the like, and the number of the bacteria can be difficult to determine by the prior art once the bacteria are overlapped more.
Therefore, how to design an automatic bacteria counting device for bacteria, which is fast, accurate and convenient, is a technical problem to be solved by the utility model.
SUMMERY OF THE UTILITY MODEL
The embodiment of the utility model provides a bacteria counting device with a sheath flow impedance sensor and a method thereof, which at least solve the technical problem that the micro-particle counting device in the prior art cannot rapidly and accurately measure the number of bacteria.
The embodiment of the utility model provides a bacteria counting device with a sheath flow impedance sensor, which comprises:
the sampling assembly is used for obtaining a bacterial sample to be counted;
the liquid storage tank, the isolation tank and the counting tank assembly are sequentially connected by pipelines;
a device for controlling the flow rate of the liquid is connected between the liquid storage tank and the isolation tank;
the counting cell component comprises a gem hole, a front cell, a rear cell and electrodes, wherein the front cell and the rear cell are communicated through the gem hole, the electrodes are respectively arranged on two sides of the gem hole, the front cell comprises front sheath liquid, the rear cell comprises rear sheath liquid, and the front sheath liquid enables the bacteria sample to be counted to enter the rear cell from the front cell through the gem hole under the combined action of a positive pressure source of the front cell and a negative pressure source of the rear cell;
and the circuit control system is used for determining the number of bacteria in the bacteria sample to be counted according to the pulse signals under the condition that the pulse signals generated on two sides of the gem hole are detected, wherein the pulse signals are used for indicating that the bacteria in the bacteria sample to be counted pass through the gem hole.
Optionally, the sheath flow impedance sensor further includes a rear sheath fluid isolation tank, the rear sheath fluid isolation tank includes a fluid inlet connected to the fluid reservoir and a fluid outlet connected to the rear tank, and a device for controlling a flow rate of the fluid is connected to the fluid inlet of the rear sheath fluid isolation tank.
Optionally, the sheath flow impedance sensor further comprises a rectifying region and an accelerating region, wherein the bacteria sample to be counted flows through the accelerating region from the front pool to the rear pool through the jewel hole under the combined action of the positive pressure source of the front pool and the negative pressure source of the rear pool in the rectifying region.
Optionally, the circuit control system includes:
the first processor is used for detecting the pulse signal, transmitting the pulse signal to a processing device and acquiring the number of bacteria in the bacteria sample to be counted, which is sent by the processing device, wherein the number of bacteria in the bacteria sample to be counted is determined according to the bacteria characteristic data represented by the pulse signal; or alternatively
The second processor is used for detecting the pulse signals and determining the number of bacteria in the bacteria sample to be counted according to the bacteria characteristic data represented by the pulse signals; and
a first power supply circuit for supplying a constant current to the gem hole via the electrodes, wherein the pulse signal is generated by one or more bacteria triggered by the gem hole when the constant current is supplied to the gem hole; or
A second power circuit for supplying a constant voltage to the jewel hole via the electrode, wherein the pulse signal is a pulse signal generated by one or more bacteria triggered through the jewel hole when the constant voltage is supplied to the jewel hole.
Optionally, the diameter of the gem hole is a diameter within a first target diameter range, wherein the first target diameter range is used for allowing only one bacterium to pass through the gem hole at a time when the bacteria in the bacteria sample to be counted pass through the gem hole; or
The diameter of the gem hole is within a second target diameter range, wherein the second target diameter range is used for allowing a plurality of bacteria to pass through the gem hole at a time when the bacteria in the bacteria sample to be counted pass through the gem hole.
Optionally, in a case where the diameter of the gem hole is within the first target diameter range, the diameter of the gem hole is 30 to 70 micrometers, and/or the length of the gem hole is 30 to 100 micrometers.
Optionally, in a case where the diameter of the gem hole is within the first target diameter range, the diameter of the gem hole is 40 to 60 microns, and/or the length of the gem hole is 40 to 70 microns.
The embodiment of the utility model also provides a counting method of the bacteria counting device with the sheath flow impedance sensor, which comprises the following steps:
adding a bacterial sample to be counted into a counting cell assembly, wherein the counting cell assembly comprises a gem hole, a front cell, a rear cell and electrodes, the front cell and the rear cell are communicated through the gem hole, the electrodes are respectively arranged on two sides of the gem hole, the front cell comprises front sheath liquid, the rear cell comprises rear sheath liquid, and the front sheath liquid enables the bacterial sample to be counted to enter the rear cell from the front cell through the gem hole under the combined action of a positive pressure source of the front cell and a negative pressure source of the rear cell; one of said electrodes being disposed on each side of said gemstone aperture, said electrode having a predetermined resistance between said electrodes when energized;
detecting whether pulse signals generated by the resistance change between the two sides of the gem hole exist on the two sides of the gem hole or not, wherein the pulse signals are used for indicating that bacteria in the bacteria sample to be counted pass through the gem hole;
and under the condition that pulse signals generated on two sides of the gem hole are detected, acquiring the number of bacteria in the bacteria sample to be counted, which is determined according to the pulse signals.
Optionally, the obtaining the number of bacteria in the bacteria sample to be counted determined according to the pulse signal includes:
transmitting the pulse signal to a processing device, and acquiring the number of bacteria in the bacteria sample to be counted, which is sent by the processing device, wherein the number of bacteria in the bacteria sample to be counted is determined according to the bacteria characteristic data represented by the pulse signal; or
And determining the number of bacteria in the bacteria sample to be counted according to the bacteria characteristic data represented by the pulse signal.
Optionally, the diameter of the gem hole is a diameter within a first target diameter range, wherein the first target diameter range is used for allowing only one bacterium to pass through the gem hole at a time when the bacteria in the bacteria sample to be counted pass through the gem hole; or
The diameter of the gem hole is within a second target diameter range, wherein the second target diameter range is used for allowing a plurality of bacteria to pass through the gem hole at a time when the bacteria in the bacteria sample to be counted pass through the gem hole.
One of the electrodes is disposed on each side of the gem hole, and the bacteria are non-conductive, so that the bacteria generate voltage pulse signals when passing through the gem hole, and the number of bacteria in the bacteria sample to be counted can be determined according to the pulse signals, and the pulse signals can be selected as voltage pulse signals.
The bacteria characteristic data represented by the pulse signal comprises: amplifying and gaining the pulse signal through a conditioning circuit, filtering noise through low-pass filtering, and filtering out an overrun amplitude value through buffer amplitude limiting; and identifying the signal with the bacteria characteristic data in the pulse signal through algorithms such as pulse identification, slope identification, peak detection, trough detection, broadband detection and the like.
Blocking phenomenon (hole blocking) appears in above-mentioned precious stone hole, divide into totally block up the hole and incompletely block up the hole, and complete blocking phenomenon appears in above-mentioned precious stone hole promptly and incomplete blocking phenomenon appears in above-mentioned precious stone hole.
If the hole is completely blocked, the counting amount is abnormal and a correct result cannot be counted, and the hole blocking phenomenon is eliminated by adopting the burning assembly; but if the incomplete hole blocking happens, the data can be displayed, the test result is directly influenced, whether the incomplete hole blocking phenomenon occurs can be distinguished from the observation counting time, namely, the observation counting time has a reference value, if the bacteria counting device works normally, the micropores are unobstructed, the time for sucking the bacteria sample to be counted is fixed, when the counting time is prolonged, the incomplete hole blocking phenomenon occurs on a detector of the bacteria counting device is shown, optionally, an algorithm is adopted to judge the number exceeding the limit when the hole blocking occurs on the other scheme, so that the data is judged to be inaccurate, and the hole blocking phenomenon is judged or the external interference is received.
Because under the device normal operating condition, the count time fixed value has been established, the count time is even, because under the normal operating condition, aperture voltage is stable in certain extent basically, if take place aperture voltage rising or count quantity unusual condition, then prove stifled hole or impurity interference phenomenon appear in above-mentioned precious stone hole, stifled hole reason has a lot of, most of cases are because multiple bacterium mixes inhomogeneous, perhaps wash the precious stone hole often, then the piling up of non-counting material can appear to produce stifled hole.
Optionally, a mode of judging whether the hole is blocked or not through a voltage interval is provided, that is, the voltage is divided into 3 grades, namely, the voltage is normal, higher or abnormal, when the voltage is higher, the hole blocking phenomenon is generated by a detector of the bacteria counting device, the higher is a micro hole blocking phenomenon (namely, an incomplete hole blocking phenomenon), the abnormal is a complete hole blocking phenomenon, and the normal is a non-hole blocking state; if the voltage of the small hole rises or the counting amount is abnormal or the base line is judged to be abnormal, the phenomenon that the gem hole is blocked or impurities or interference occur is proved.
Further, the above counting cell assembly may further optionally comprise:
and the burning component is used for burning and eliminating the blockage of the gem hole when the blockage phenomenon of the gem hole occurs.
Further, the burning assembly is used for providing a voltage higher than a preset voltage value to the gem hole through the electrode when the blocking phenomenon occurs to the gem hole so as to melt the hole blocking substances in the gem hole.
After the computer connected with the bacteria counting device detects the hole blockage, namely the alarm or prompt information of the computer, the computer can artificially execute high-voltage ignition to eliminate the hole blockage, namely, the operation button on the computer (PC end) is artificially clicked, a high-voltage ignition circuit is started, namely, direct current voltage (relative low-voltage part) is normally counted, direct current high voltage is generated during ignition, the ignition mode is high-voltage and low-voltage rapid switching, high frequency can be formed in the high-voltage ignition process, arc discharge can be generated on two sides of a jewel hole at the moment of power on and power off, and generated electric sparks just burn hole blockage substances in the jewel hole. Another optional way of burning to eliminate the hole blockage is that when normal counting is to provide stable low-voltage partial pressure through a switch circuit, direct current high voltage is used for burning, and because the high voltage is used for burning, the liquid to be detected is heated and boiled, and protein components are melted and eliminated to achieve the effect of burning to eliminate the hole blockage.
Further, the voltage of the predetermined voltage value is 90 volts to 110 volts.
Further, the voltage of the predetermined voltage value is 110 v.
Further, the forebay is made of plastic materials.
Further, the forebay is made of polyformaldehyde material.
Further, the rear tank is made of plastic materials.
Further, the rear pool is made of a polyformaldehyde material.
The plastic material, especially polyoxymethylene material machine working property is good, guarantee the size of above-mentioned front pool and above-mentioned rear pool easily, the structure is more firm.
The technical scheme provided by the embodiment of the utility model has the following beneficial effects:
1) the embodiment of the utility model realizes the automatic application of the device for measuring the number of bacteria by using a resistance counting method, solves the problems of slow time and low efficiency of the existing bacteria counting, and realizes the effects of high speed and accuracy of bacteria counting.
2) The improved jewel holes of the embodiment of the utility model ensure that bacteria can pass through the micropores one by one, prevent the overlapping phenomenon from influencing the measurement of the number of the bacteria, realize the measurement of the number of the bacteria by adopting a resistance counting method, and have accuracy and high efficiency; add high pressure firing function prevention stifled hole, if have stifled hole phenomenon, can select the firing function to eliminate stifled hole, ensure promptly under the circumstances that the aperture diminishes that be difficult to appear above-mentioned precious stone hole totally block up the phenomenon or above-mentioned precious stone hole incompletely blocks up the phenomenon.
3) A bacteria counting signal conditioning circuit is designed in a targeted manner; and a signal conditioning circuit is added, so that non-bacterial signals are filtered, signals of bacterial characteristics are accurately identified, and the condition of misjudgment is reduced.
4) The counting performance of the sheath flow impedance counting sensor is better than that of impedance method bacterial counting, the technical problem that the measured quantity may be inaccurate due to the fact that the original impedance counting pool exposes the measured particles and the particles cannot pass through the gem hole singly sometimes and the generated M wave cannot be distinguished well can be solved well by sheath flow impedance counting, the measured particles are focused in the sheath flow chamber, the particles to be measured form liquid flow which is about the size of the measured particles, and therefore the particles regularly pass through the counting part of the small hole part.
Drawings
The above and other objects, features and advantages of exemplary embodiments of the present invention will become readily apparent from the following detailed description read in conjunction with the accompanying drawings. Several embodiments of the utility model are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings and in which:
FIG. 1-1 schematically illustrates a complete schematic view of a bacteria counting apparatus according to an embodiment of the present invention;
FIGS. 1-2 schematically illustrate a schematic drawing of a complete machine motion pipetting process for a bacteria counting apparatus according to an embodiment of the present invention;
FIGS. 1-3 schematically illustrate an internal structural view of a count shield box according to an embodiment of the present invention;
FIG. 2 schematically illustrates a cross-sectional structure of a gemstone hole, in accordance with an embodiment of the utility model;
FIG. 3 schematically shows a schematic representation of the structure of an incubation site according to an embodiment of the utility model;
FIG. 4 schematically illustrates a flow diagram of a signal conditioning circuit according to an embodiment of the present invention;
FIG. 5 schematically illustrates a schematic view in partial cross-section of a counting cell assembly with a sheath flow impedance sensor, in accordance with an embodiment of the utility model;
FIG. 6 schematically illustrates a back sheath fluid and front sheath fluid strike schematic, in accordance with an embodiment of the present invention;
FIG. 7 schematically illustrates a front sheath fluid in a front reservoir and a back sheath fluid in a back reservoir oriented in accordance with an embodiment of the present invention;
fig. 8 schematically shows a schematic diagram of the operation of a liquid circuit of a bacteria counting device with a sheath flow impedance sensor according to an embodiment of the present invention.
Detailed Description
The principles and spirit of the present invention will be described with reference to a number of exemplary embodiments. It is understood that these embodiments are given solely for the purpose of enabling those skilled in the art to better understand and to practice the utility model, and are not intended to limit the scope of the utility model in any way. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
The embodiment of the utility model provides a bacteria counting device with a sheath flow impedance sensor, which comprises:
the sampling assembly is used for obtaining a bacterial sample to be counted;
the liquid storage tank, the isolation tank and the counting tank assembly are sequentially connected by pipelines;
a device for controlling the flow rate of liquid is connected between the liquid storage tank and the isolation tank;
the counting cell component comprises a gem hole, a front cell, a rear cell and electrodes, wherein the front cell and the rear cell are communicated through the gem hole, the electrodes are respectively arranged on two sides of the gem hole, the front cell comprises front sheath liquid, the rear cell comprises rear sheath liquid, and the front sheath liquid enables the bacteria sample to be counted to enter the rear cell from the front cell through the gem hole under the combined action of a positive pressure source of the front cell and a negative pressure source of the rear cell;
and the circuit control system is used for determining the number of bacteria in the bacteria sample to be counted according to the pulse signals under the condition that the pulse signals generated on two sides of the gem hole are detected, wherein the pulse signals are used for indicating that the bacteria in the bacteria sample to be counted pass through the gem hole.
By the inlet position department at above-mentioned isolation pond or be equipped with the device of control liquid velocity of flow near the inlet position department of isolation pond, the liquid that flows from the inlet has been carried out the deceleration effectively and has been handled, the velocity of flow that makes the liquid of adding touch isolation pond inner wall or isolation bottom of the pool liquid level is less than the velocity of flow when liquid just flowed the inlet greatly, greatly reduced the impact of the liquid of adding to isolation pond inner wall or isolation bottom of the pool liquid level, thereby greatly reduced the probability of producing the bubble, the interference of bubble to the counter has been reduced, the accuracy that the instrument detected has been improved greatly.
The overall schematic diagram of the bacteria counting device is shown in fig. 1-1, and as shown in fig. 1-1 and fig. 1-2, the bacteria counting device includes a counting shielding box 1, a sampling assembly 2, an incubation position 24, a plunger pump 25, a signal conditioning circuit 3, and a housing 4, the sampling assembly 2 includes a sampling needle 22, the housing 4 includes a cableway 41, the sampling assembly 2, the signal conditioning circuit 3, and the counting shielding box 1 are disposed in the housing 4, the incubation position 24 of the bacteria counting device is also disposed in the housing 4, the incubation position 24 may be 10 or 20 or more, and the structure may be changed accordingly, for example, the structure may be changed into the structure of the hole position of the incubation position 24 shown in fig. 3, where the hole 241 is a place for placing a sample container tube to be tested.
The counting shield box 1 includes a counting cell assembly, as shown in fig. 1 to 3 and 8, the counting cell assembly is specifically a sheath flow counting cell Q16 in this embodiment, the sheath flow counting cell Q16 is fixedly connected to the sampling assembly 2, the counting shield box 1 further includes a back sheath fluid isolation cell Q9, a pre-mixing cell Q10, a sheath fluid reservoir Q11, a sheath flow counting cell Q16 and a waste fluid isolation cell Q7, the pre-mixing cell Q10, the sheath fluid reservoir Q11, the back sheath fluid isolation cell Q9, the sheath flow counting cell Q16 and the waste fluid isolation cell Q7 are sequentially connected by a pipeline, that is, the reservoir is specifically the sheath fluid reservoir Q11 in this embodiment, the isolation cell is specifically the back sheath fluid isolation cell Q9 in this embodiment, as shown in fig. 8, one end of the back sheath fluid isolation cell Q9 is connected to the sheath fluid reservoir Q11 by a connection channel, the front cell inlet of the sheath flow counter Q16 is connected to the sheath fluid reservoir Q11, and the rear sheath fluid separator Q9 is connected to the rear sheath fluid inlet Q1 of the sheath flow counter Q16 shown in fig. 5.
The signal conditioning circuit 3 is shown in fig. 4, the signal conditioning circuit 3 is disposed under the cableway 41 shown in fig. 1-2, that is, disposed in the bacteria counting device, the signal conditioning circuit 3 is connected to the inner electrode and the outer electrode in the counting cell assembly (sheath flow counting cell Q16), the signal conditioning circuit 3 includes a signal collecting board, a main control board, and the like, the housing 4 is disposed outside the counting cell assembly (sheath flow counting cell Q16), the sampling assembly 2 and the signal conditioning circuit 3, wherein the sampling assembly 2 includes a moving mechanism, the sampling assembly 2 collects the bacteria solution to be measured through the moving mechanism and places the bacteria solution into the counting cell assembly (sheath flow counting cell Q16), and the counting cell assembly (sheath flow counting cell Q16) is driven by the sampling assembly 2 to slide on the cableway 41.
A schematic cross-sectional structure of the sheath flow counting cell Q16 is shown in fig. 5, the sheath flow counting cell Q16 includes a three-way tube Q161, a front cell 12, an accelerating section Q162, a front sheath liquid inlet Q163, a hose connector Q164, a jewel hole 11, a sealing ring Q165, a rear cell 13, a rear sheath liquid inlet Q1, a capturing tube Q166, and a sample needle Q167, which are connected as shown in fig. 5, wherein the sample needle Q167 is a channel for a sample liquid, the sample liquid is introduced from the three-way tube Q161, as shown in fig. 8, the three-way tube Q161 is configured as a connector connecting V6, V7 and the sheath flow counting cell, one channel of the three-way tube Q161 is connected to V6, the other channel is connected to V7, the other channel is connected to the sample needle Q167 as shown in fig. 5, the sample needle Q167 is embedded inside the front cell 12, the liquid at the front sheath liquid inlet Q163 can only go from outside to inside, the sample liquid is introduced into the accelerating section Q162 by the three-way tube Q161 and the accelerating section Q167, the front pool 12 and the rear pool 13 are made of plastic materials, and the sheath flow and the sheath fluid in the embodiment mean the same meaning, in which the rear sheath flow enters the portion of the rear pool 13 through the gem hole 11, and the rear sheath flow is communicated with the catching pipe Q166 only from the outside to the inside, wherein the front sheath flow enters from the front sheath fluid inlet Q163, the rear sheath flow enters from the rear sheath fluid inlet Q1.
The intensity of the measuring signal of the inner electrode and the outer electrode is a receptor for counting bacteria. Because the diluent has conductivity, when a certain voltage is applied between the two electrodes, a certain resistance exists between the micropores of the gem hole 11, and the cells have non-conductivity, when the cells enter the pores, the resistance between the pores can be changed, so that a pulse signal is generated in the circuit, the pulse signal is processed and transmitted to the PC end for analysis, parameters such as the number, the size and the like of the cells can be measured and counted according to the characteristics such as the number of the pulses, the pulse amplitude and the like, the working principle diagram is shown as figure 4, the bacterial number of the bacterial liquid to be measured is obtained through the resistance counting of the bacterial counting device, and the bacterial number is transmitted to the PC (computer) end.
The schematic structural diagram of the signal conditioning circuit 3 is shown in fig. 4, and the signal conditioning circuit on the signal processing board collects micro signals, and then uploads the number of bacteria through amplification filtering, signal collection and the like.
As shown in fig. 1-1, 1-2 and 1-3, when the bacteria counting apparatus is operated, the sampling assembly 2 moves rapidly to a predetermined position, the plunger pump 25 controls the sampling needle 22 to initially mix the bacteria sample solution in the incubation position 24 and suck the bacteria sample solution, and then the bacteria sample solution is discharged into the forebay 12 of the sheath flow counting chamber Q16 which moves together with the sampling assembly 2.
Optionally, as shown in fig. 2, in order to take account of the test signal intensity and the counting time of bacteria, a structural diagram of the gemstone hole 11 is shown, where a gemstone hole diameter 111 (gemstone hole diameter) of the gemstone hole 11 is set in a range from 30 micrometers to 70 micrometers, preferably from 40 micrometers to 60 micrometers, a length 112 of the gemstone hole 11 is from 30 micrometers to 100 micrometers, preferably from 40 micrometers to 70 micrometers, the gemstone hole diameter 111 is 50 micrometers, and the length 112 of the gemstone hole is 50 micrometers, which is most suitable for measurement of bacteria.
The test effect data of the resistance counting of different gem hole apertures of the same standard bacteria liquid to be tested (about 2500 standard bacteria/mL) by using the design is as follows:
1) the measured number of bacteria per ml (after conversion) for different pore sizes when the gemstone pore length is 50 microns is shown in table 1.
TABLE 1
| Number/aperture | 30um | 40um | | 60um | 70um | |
| 1 | 1035 | 2132 | 2501 | 1142 | 936 | |
| 2 | 1056 | 2200 | 2488 | 1132 | 921 | |
| 3 | 1034 | 2150 | 2493 | 1135 | 902 | |
| 4 | 1026 | 2140 | 2487 | 1136 | 910 | |
| 5 | 1019 | 2157 | 2495 | 1156 | 930 | |
| 6 | 1034 | 2169 | 2510 | 1158 | 940 |
Analysis proves that the pore diameter of the gem hole is too small, the phenomenon of hole blocking can occur, the measured particle number is reduced, if the pore diameter of the gem hole is larger, the particle number passing the same time is increased, counting is inaccurate, and the measured particle number is also reduced. As can be seen from the above table, the above-mentioned gem pore diameter 111 is optimal when it is 50 micrometers, i.e. the test effect data of the resistance counting is the best.
2) When the diameter of the gem pore is set to 50 microns, the number of bacteria per ml (after conversion) measured for different gem pore lengths is shown in table 2.
TABLE 2
| Serial number/length | 30um | 40um | 50um | 60um | 70um | | 90um | 100um | |
| 1 | 1700 | 1800 | 2501 | 1650 | 1025 | 951 | 850 | 725 | |
| 2 | 1750 | 1800 | 2488 | 1645 | 1030 | 988 | 845 | 730 | |
| 3 | 1705 | 1805 | 2493 | 1651 | 1050 | 993 | 851 | 750 | |
| 4 | 1725 | 1795 | 2487 | 1648 | 1064 | 987 | 848 | 764 | |
| 5 | 1736 | 1796 | 2495 | 1646 | 1036 | 995 | 846 | 736 | |
| 6 | 1710 | 1810 | 2510 | 1652 | 1037 | 910 | 852 | 737 |
Analysis proves that under the condition that the aperture of the gem hole is not changed, if the length of the gem hole is longer, the number of particles passing through the same time is increased, so that inaccurate counting is caused, and the measured number of particles is reduced; if the length of the gem hole is too short, many particles can not be tested due to fast flow speed, so that the number of the measured particles can be reduced; as can be seen from the above table, the above-mentioned gem pore length is optimal at 50 μm, i.e. the test effect data of the resistance counting is the best.
As can be seen from the above table, the aperture 111 of the gemstone hole is 50 micrometers, and the length 112 of the gemstone hole is 50 micrometers, which is optimal, that is, the test effect data of resistance counting is the best, although the counting effect of the gemstone hole and the gemstone hole in other ranges is not 50 micrometers, the gemstone hole and the gemstone hole can still count, and the caused inaccurate counting is relatively speaking, that is, the trend judgment of the bacterial count of the bacterial liquid to be measured is still accurate for different bacterial liquids to be measured by using the same counting standard, which indicates that the specifications of other gemstone hole and hole can still measure the magnitude of bacterial count.
Optionally, two platinum electrodes are respectively arranged on two sides of the laser-formed gem hole, because the diluent has conductivity, when a certain voltage is applied between the two electrodes, a certain resistance is arranged between the micropores, and the cells have non-conductivity, when the cells enter the micropores, the resistance between the micropores can be changed, so that a pulse signal is generated in the circuit, the pulse signal is processed and transmitted to the PC end for analysis, and parameters such as the number, the size and the like of the cells can be measured and counted according to the number of the pulses, the characteristics such as the pulse amplitude and the like.
A bacterial count signal conditioning circuit and an acquisition algorithm are designed in a targeted manner, effective signals are completely reserved through amplifying the signals, and the effective signals are adjusted to the amplification times which are most beneficial to algorithm identification through adjusting gains; the low-pass filtering filters high-frequency noise, the amplitude exceeding the limit is filtered through buffer amplitude limiting, and the signal of the bacteria characteristic is accurately identified through algorithms such as pulse identification, slope identification, wave crest detection, wave trough detection, broadband detection and the like, so that the bacteria quantity is obtained from the pulse signal.
In the event that said pulse signals comprise a first type of set of pulse signals, said circuit control system or processing device determining a first number of said first type of set of pulse signals, wherein each pulse signal of said first type of set of pulse signals is a pulse signal triggered by one of said bacteria through said gemstone aperture; in the case where the pulse signal includes a second type of pulse signal, the circuit control system or the processing device determines a second number as a product of a number of the second type of pulse signal and a predetermined number, wherein each of the second type of pulse signal is a pulse signal generated by the predetermined number of bacteria simultaneously passing through the jewel hole.
Determining the number of bacteria in said sample of bacteria to be counted as said first number in case said pulse signals comprise only one set of pulse signals of said first type; determining the number of bacteria in said sample of bacteria to be counted as said second number in case said pulse signals comprise only one set of pulse signals of said second type; in the case where the pulse signal includes a set of pulse signals of the first type and a set of pulse signals of the second type, the number of bacteria in the bacterial sample to be counted is determined as the sum of the first number and the second number.
As an example, the circuit control system or the processing device in the embodiment of the present invention may determine whether the pulse signal includes a group of pulse signals of the second type by:
the bacteria counting device can count the bacteria according to a group of pulse signals of the first type under the action of sheath flow liquid, so that the bacteria can count according to a group of pulse signals of the first type, namely the bacteria can be accurately counted when only 1 bacterium can pass through the bacteria, 2 bacteria or 3 bacteria simultaneously pass through the gem hole, the second type pulse signals are recorded as effective counts when the second type pulse signals and the first type pulse signals are within an error range, otherwise, errors are reported and the counts are counted again or counting results are converted according to error values.
As an example, during the process of bacteria passing through the jewel aperture, the jewel aperture may be clogged, thereby causing inaccurate counting of bacteria. In order to solve the problem of inaccurate counting of bacteria caused by blockage of the jewel hole, the embodiment of the utility model also provides a detection scheme and a removal scheme of the blockage of the jewel hole.
Of course, the bacteria counting device with the sheath flow impedance sensor provided by the embodiment of the utility model basically cannot suffer from the hole blockage phenomenon, because the sheath flow technology is introduced into the bacteria counting device provided by the utility model, so that bacteria can pass through the gem hole in a queuing mode, and a well can pass through the gem hole in a good condition without causing the hole blockage phenomenon.
However, as an exemplary detection scheme for the blockage of the jewel hole, the embodiment of the utility model further comprises: determining that the gem hole is blocked when the voltage between the front pool and the rear pool is detected to exceed a predetermined threshold, wherein the front pool is an anode and the rear pool is a cathode, the more the blockage of the gem hole is serious, the larger the resistance between two sides of the gem hole is, and the larger the voltage between the front pool and the rear pool is, and the predetermined threshold can be set according to different measurement requirements (for example, different measurement precision) of the number of bacteria.
As an exemplary solution for eliminating the plugged hole by burning, the embodiment of the present invention further includes: and (4) high-pressure burning.
The firing process is under 110V voltage, high-frequency counting voltage is added on the electrodes at two ends of the small hole, during normal counting, the counting voltage is direct current voltage continuously provided, during high-voltage firing, the high-frequency counting electrode is designed to be powered on and powered off at short intervals, so that high frequency is formed, at the moment of power on and power off, arc discharge is generated between the two electrodes, the emitting point of electric sparks is the small hole, so that protein and fragments are easily removed, other instruments are designed to be independently powered by alternating current, and the front end of an electrode wire is controlled by a relay or a silicon controlled rectifier. Optionally, the method can also be used for burning and eliminating the hole blocking phenomenon by a mode of boiling and heating to melt the protein under high pressure.
Optionally, after the application of sample needle injected liquid into the forebay, the negative pressure effect brought forebay liquid to the back pond, pass through the precious stone hole like this, when the bacterium passed through precious stone hole voltage, just can produce pulse signal (what provided is the constant current source, the bacterium changes then the voltage changes through representing resistance change), the circuit is through the filtering, the signal amplification, then at the filtering, the signal reaches the singlechip, the singlechip carries out the process of AD sampling, the singlechip procedure also has pulse recognition algorithm, after handling, just can upload the PC software. And the process of processing the signals comprises the following steps: the AD samples are sampled approximately 10M magnitude and then the algorithm processes to a number of K, i.e. total, histogram information, which is then uploaded to the PC.
Optionally, in the working process of the bacteria counting apparatus, as shown in a liquid path diagram of fig. 8, before sample addition and counting, before each counting, the sheath flow counting cell Q16 is cleaned, first, the sample liquid is sucked from the incubation position 24 to the pre-mixing cell Q10 by the sampling needle 22, diluted, and the valves V6 and V7 are opened, so that the liquid to be measured is sucked from the pre-mixing cell Q10 to the pipeline under the action of the negative pressure source Q13 and the negative pressure waste liquid cell Q8, and then the valves V6 and V7 are closed; opening a V1 valve, supplying liquid from a sheath liquid storage pool Q11 to a rear sheath liquid isolation pool Q9 by using a positive pressure source Q12, closing a V1 valve before the liquid in the rear sheath liquid isolation pool Q9 reaches the top, opening a V5 valve, and allowing the liquid in the rear sheath liquid isolation pool Q9 to enter a counting pool through a flow limiting pipe Q14; opening a V2 valve, introducing the liquid in a sheath liquid storage pool Q11 into a front pool as a front sheath flow through a V2 valve, enabling the front sheath flow to pass through small holes under the combined action of a positive pressure source Q12 of the front pool and a negative pressure source Q13 of a rear pool, opening a valve V4, pushing the liquid to be measured in a pipeline Q15 into a counting pool by a pump, enabling the liquid to be measured Q6 to converge, gradually thin and accelerate at an outlet of the front pool 12 under the action of the front sheath liquid Q3, and then sequentially passing through the small holes under the wrapping of the front sheath liquid Q3 along an axis, as shown in figures 6, 7 and 8, when the liquid to be measured Q6 enters the rear pool 13, enabling a rear sheath liquid Q2 to enter from a rear sheath liquid inlet Q1, and enabling the liquid to be measured Q6 to sequentially flow away in the direction of a rear sheath liquid Q2.
The experimental comparison of the bacteria counting device with the sheath flow impedance sensor and the electrical impedance bacteria counting device without the sheath flow impedance sensor in the embodiment of the utility model is as follows:
by contrast, the bacteria liquid sheath flow number measured by the bacteria counting device with the sheath flow impedance sensor in the embodiment of the utility model is obviously higher than that measured by an electrical impedance bacteria counting device without the sheath flow impedance sensor.
The mie value (turbidity value) of the bacterial liquid was 0.5, and the 2-hour test data of the electrical impedance bacteria counting device without the sheath flow impedance sensor is shown in table 3:
TABLE 3
The Mylar (turbidity) value of the inoculum solution was 0.5 and the 2 hour test data for the bacteria counting device with sheath flow impedance sensor is shown in Table 4:
TABLE 4
Therefore, as can be seen from the results in tables 3 and 4, the bacteria counting apparatus having the sheath flow impedance sensor measured the number of bacteria significantly higher than the bacteria counting apparatus having no sheath flow impedance sensor, and the bacteria counting apparatus having the sheath flow impedance sensor measured the number of bacteria more accurately.
When a sample/liquid to be detected is injected, two straight ends of the T-shaped tee joint are injected, and the T-shaped ends are connected with a sample needle (filled with the sample/liquid to be detected), so that the sample preparation time is shortened;
the sheath flow impedance sensor and the fluid contact material are both corroded by a strong alkaline resistant reagent, and the compatibility with a probe cleaning liquid reagent is met.
The rear sheath liquid isolation pool Q9 is used for providing the sheath liquid of the rear pool of the sheath flow counting pool Q16, and the rear sheath liquid isolation pool Q9 comprises a pool body, a liquid inlet, a liquid outlet, a gas inlet and a float sensor;
in the measurement process, liquid in the cell body of the back sheath liquid isolation cell Q9 can keep a certain liquid level height, the back sheath liquid is driven by gravity, and gas is prevented from entering the back cell to influence counting; the liquid inlet is effectively isolated from the liquid level in the tank in the measuring process; the liquid outlet is connected with a proper throttle pipe.
The waste liquid isolation pool Q7 is used for a waste liquid pool for isolating the waste liquid in the rear pool of the counting pool from other liquid, and the waste liquid isolation pool Q7 can normally drain liquid and normally inject bubbles; the material of the waste liquid isolation pool Q7 must be a chemically inert material; a liquid inlet is reserved at the upper end of the waste liquid isolation tank Q7 and is connected with a waste liquid discharge port of the sensor rear tank; the waste liquid isolation pool Q7 has a overflow port connected with the atmosphere; the waste liquid isolation pool Q7 has a liquid discharge port for discharging waste liquid.
The volume between the lower port and the bottom of the upper end liquid inlet pipe of the waste liquid isolation pool Q7 is larger than the liquid consumption of the channel during measurement; the waste liquid isolation tank Q7 requires that the isolation chamber and the counting tank are reasonably and reliably connected and have no air leakage or liquid leakage; and in order to ensure the isolation quality, a liquid inlet of the isolation chamber extends into the chamber by 10mm in the waste liquid isolation tank Q7.
It should be noted that although several units of the apparatus are mentioned in the above detailed description, such division is merely exemplary and not mandatory. Indeed, the features and functions of two or more of the units described above may be embodied in one unit, according to embodiments of the utility model. Conversely, the features and functions of one unit described above may be further divided into embodiments by a plurality of units.
Moreover, while the operations of the method of the utility model are depicted in the drawings in a particular order, this does not require or imply that the operations must be performed in this particular order, or that all of the illustrated operations must be performed, to achieve desirable results. Additionally or alternatively, certain steps may be omitted, multiple steps combined into one step execution, and/or one step broken down into multiple step executions.
While the spirit and principles of the utility model have been described with reference to several particular embodiments, it is to be understood that the utility model is not limited to the disclosed embodiments, nor is the division of aspects, which is for convenience only as the features in such aspects may not be combined to benefit. The utility model is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims.
Claims (7)
1. A bacteria counting device having a sheath flow impedance sensor, comprising:
the sampling assembly is used for obtaining a bacterial sample to be counted;
the liquid storage tank, the isolation tank and the counting tank assembly are sequentially connected by pipelines;
a device for controlling the flow rate of liquid is connected between the liquid storage tank and the isolation tank;
the counting cell component comprises a sheath flow impedance sensor, the sheath flow impedance sensor comprises a gem hole, a front cell, a rear cell and electrodes, wherein the front cell and the rear cell are communicated through the gem hole, the electrodes are respectively arranged on two sides of the gem hole, the front cell comprises front sheath liquid, the rear cell comprises rear sheath liquid, and the front sheath liquid enables the bacteria sample to be counted to enter the rear cell from the front cell through the gem hole under the combined action of a positive pressure source of the front cell and a negative pressure source of the rear cell;
and the circuit control system is used for determining the number of bacteria in the bacteria sample to be counted according to the pulse signals under the condition that the pulse signals generated on two sides of the gem hole are detected, wherein the pulse signals are used for indicating that the bacteria in the bacteria sample to be counted pass through the gem hole.
2. The bacteria counting device of claim 1,
sheath flow impedance sensor still includes back sheath liquid isolation pool, back sheath liquid isolation pool is including being connected to the inlet of liquid reserve tank with be connected to the liquid outlet of back pond the inlet department of back sheath liquid isolation pool is connected with the device that is used for controlling the liquid velocity of flow.
3. The bacteria counting device of claim 2,
the sheath flow impedance sensor also comprises a rectifying area and an accelerating area, wherein the bacteria sample to be counted flows through the accelerating area under the combined action of the positive pressure source of the front pool and the negative pressure source of the rear pool by the front sheath liquid in the rectifying area, and then flows from the front pool to the rear pool through the jewel hole.
4. The apparatus of claim 1, wherein the circuit control system comprises:
the first processor is used for detecting the pulse signal, transmitting the pulse signal to a processing device and acquiring the number of bacteria in the bacteria sample to be counted, wherein the number of bacteria in the bacteria sample to be counted is determined according to the bacteria characteristic data represented by the pulse signal; or alternatively
The second processor is used for detecting the pulse signals and determining the number of bacteria in the bacteria sample to be counted according to the bacteria characteristic data represented by the pulse signals; and
a first power circuit for supplying a constant current to the gemstone aperture through the electrode, wherein the pulse signal is a pulse signal generated by one or more of the bacteria triggered through the gemstone aperture while the constant current is supplied to the gemstone aperture; or
A second power circuit for supplying a constant voltage to the gemstone aperture through the electrode, wherein the pulsed signal is a pulsed signal generated by one or more of the bacteria triggered through the gemstone aperture while the constant voltage is supplied to the gemstone aperture.
5. The device of any one of claims 1 to 4, wherein the diameter of the gemstone well is a diameter within a first target diameter range, wherein the first target diameter range is for allowing only one bacterium to pass through the gemstone well at a time when the bacterium in the bacterial sample to be enumerated passes through the gemstone well; or
The diameter of the gem hole is a diameter within a second target diameter range, wherein the second target diameter range is used for allowing a plurality of bacteria to pass through the gem hole at a time when the bacteria in the bacteria sample to be counted pass through the gem hole.
6. The apparatus of claim 5, wherein the diameter of the gemstone hole is 30 to 70 microns where the diameter of the gemstone hole is within a first target diameter range, and/or the length of the gemstone hole is 30 to 100 microns.
7. The apparatus of claim 6, wherein the diameter of the gemstone hole is 40 to 60 microns where the diameter of the gemstone hole is within a first target diameter range, and/or the length of the gemstone hole is 40 to 70 microns.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010791059 | 2020-08-07 | ||
| CN2020107910592 | 2020-08-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216594694U true CN216594694U (en) | 2022-05-24 |
Family
ID=80233476
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121837963.9U Active CN216594694U (en) | 2020-08-07 | 2021-08-06 | Bacterium counting assembly with sheath flow impedance sensor |
| CN202110889383.2A Pending CN114062228A (en) | 2020-08-07 | 2021-08-06 | Bacteria counting device with sheath flow impedance sensor and method thereof |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110889383.2A Pending CN114062228A (en) | 2020-08-07 | 2021-08-06 | Bacteria counting device with sheath flow impedance sensor and method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN216594694U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114062228A (en) * | 2020-08-07 | 2022-02-18 | 北京鑫骥金诺医疗器械有限公司 | Bacteria counting device with sheath flow impedance sensor and method thereof |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5194909A (en) * | 1990-12-04 | 1993-03-16 | Tycko Daniel H | Apparatus and method for measuring volume and hemoglobin concentration of red blood cells |
| JPH09318522A (en) * | 1996-05-29 | 1997-12-12 | Toa Medical Electronics Co Ltd | Particle detector and partcle analyzer |
| EP1162449A1 (en) * | 2000-06-06 | 2001-12-12 | Universiteit Gent | AC-based gate detection method and device for the sizing of colloidal particles, cells and bacteria in liquids |
| CN101173887B (en) * | 2006-11-02 | 2011-04-06 | 深圳迈瑞生物医疗电子股份有限公司 | Particle analyzer of sheath-flow impedance method |
| CN102564918B (en) * | 2010-12-31 | 2015-12-02 | 深圳迈瑞生物医疗电子股份有限公司 | Particle counting device and blood cell analysis device |
| JP5951527B2 (en) * | 2013-03-07 | 2016-07-13 | 株式会社東芝 | Specimen detection apparatus and detection method |
| CN205246501U (en) * | 2015-12-21 | 2016-05-18 | 深圳联开生物医疗科技有限公司 | Stifled system and hematology analyzer are arranged in firing of balanced formula of intelligence high pressure |
| JP6688089B2 (en) * | 2016-01-22 | 2020-04-28 | 日本光電工業株式会社 | Flow cell and particle analyzer |
| CN106769805B (en) * | 2017-01-22 | 2019-06-18 | 江西特康科技有限公司 | Cell count sorter |
| CN108663303A (en) * | 2018-07-27 | 2018-10-16 | 北京指真生物科技有限公司 | A kind of blood analyser |
| CN209043773U (en) * | 2018-08-24 | 2019-06-28 | 深圳市帝迈生物技术有限公司 | Micropore plug-hole detection device, blood cell analyzer |
| CN110857910A (en) * | 2018-08-24 | 2020-03-03 | 深圳市帝迈生物技术有限公司 | Micropore blockage detection device and method and blood cell analyzer |
| CN216594694U (en) * | 2020-08-07 | 2022-05-24 | 北京鑫骥金诺医疗器械有限公司 | Bacterium counting assembly with sheath flow impedance sensor |
-
2021
- 2021-08-06 CN CN202121837963.9U patent/CN216594694U/en active Active
- 2021-08-06 CN CN202110889383.2A patent/CN114062228A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114062228A (en) * | 2020-08-07 | 2022-02-18 | 北京鑫骥金诺医疗器械有限公司 | Bacteria counting device with sheath flow impedance sensor and method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114062228A (en) | 2022-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2381285C (en) | Particle characterisation apparatus | |
| EP2804005A1 (en) | Apparatus for measuring blood cells and immunity from whole blood | |
| CN216594694U (en) | Bacterium counting assembly with sheath flow impedance sensor | |
| US20190391171A1 (en) | Blood measuring device control method | |
| CN108474725B (en) | Biological sample imaging device and biological sample imaging method | |
| CN111781053B (en) | Water sample pretreatment device, water sample pretreatment system and water sample pretreatment method | |
| CN105388099A (en) | Urine analyzing device and method for analyzing atypical cells in urine or body fluid | |
| US7968052B2 (en) | Detection and subsequent removal of an aperture blockage | |
| CN109959549A (en) | Sample testing method and sample analyser | |
| CN111876316A (en) | Bacteria counting device and method | |
| CN102564918B (en) | Particle counting device and blood cell analysis device | |
| US20180172717A1 (en) | Analysis apparatus | |
| EP0154644B1 (en) | Method and apparatus for removing foreign matter from a flow cell of a particle study device | |
| CN116148493A (en) | Blood sample distribution method and blood sample distribution device | |
| CN111893035A (en) | Bacteria counting device with indicating blind operating mechanism | |
| CN111893032A (en) | Bacteria counting device with air cleaning mechanism | |
| CN111893036A (en) | Bacterium counting assembly with bubble mixing mechanism | |
| CN111893037A (en) | Bacteria counting device with multi-probe movement mechanism | |
| WO2019000392A1 (en) | Reaction assembly, sample analyzer, and mixing method | |
| WO2020211835A1 (en) | Method for measuring bacterial inhibition of antibacterial drug, and bacterial counting device and method thereof | |
| CN111893033A (en) | Bacteria counting device with non-cleaning mechanism | |
| CN106644897A (en) | Counting apparatus for particle counting standard substance | |
| CN208621404U (en) | A kind of sample introduction automatic filtering unit | |
| CN111893034A (en) | Bacteria counting device with conduction heating mechanism | |
| US20220364963A1 (en) | Differential dispensing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220829 Address after: Room 201, Shandong Xinke Biotechnology Co., Ltd., No. 20, Weier Road, Fenghuang Industrial Park, Dongchangfu District, Liaocheng City, Shandong Province, 252024 Patentee after: Liaocheng Xinke Jinnuo Medical Equipment Co.,Ltd. Address before: 100176 room 603, 6th floor, building 5, yard 15, Ronghua South Road, economic and Technological Development Zone, Daxing District, Beijing Patentee before: BEIJING XINJI JINNUO MEDICAL EQUIPMENT Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230614 Address after: 518000 2938, Block B, Tianxia International Center, No. 8, Taoyuan Road, Dawangshan Community, Nantou Street, Nanshan District, Shenzhen, Guangdong Province (settled in Shenzhen Gongbang Business Secretary Co., Ltd.) Patentee after: Shenzhen Aier Biotechnology Co.,Ltd. Address before: Room 201, Shandong Xinke Biotechnology Co., Ltd., No. 20, Weier Road, Fenghuang Industrial Park, Dongchangfu District, Liaocheng City, Shandong Province, 252024 Patentee before: Liaocheng Xinke Jinnuo Medical Equipment Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240124 Address after: Unit 728-D1, 7th Floor, Building 18, No. A7 Tongji Middle Road, Beijing Economic and Technological Development Zone, Daxing District, Beijing, 100050 Patentee after: Beijing Xingyuanhui Technology Co.,Ltd. Country or region after: China Patentee after: Tang Mingzhong Address before: 518000 2938, Block B, Tianxia International Center, No. 8, Taoyuan Road, Dawangshan Community, Nantou Street, Nanshan District, Shenzhen, Guangdong Province (settled in Shenzhen Gongbang Business Secretary Co., Ltd.) Patentee before: Shenzhen Aier Biotechnology Co.,Ltd. Country or region before: China |