CN216260715U - Microsphere coating reaction kettle - Google Patents
Microsphere coating reaction kettle Download PDFInfo
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- CN216260715U CN216260715U CN202122067608.4U CN202122067608U CN216260715U CN 216260715 U CN216260715 U CN 216260715U CN 202122067608 U CN202122067608 U CN 202122067608U CN 216260715 U CN216260715 U CN 216260715U
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Abstract
The utility model relates to the field of microsphere coating production, in particular to a microsphere coating reaction kettle. The utility model relates to a microsphere-coated reaction kettle, which comprises: the device comprises a kettle body, a kettle cover, a stirrer, a motor, a parameter monitoring assembly and a temperature adjusting device, wherein the kettle cover is arranged at the top of the kettle body; when the temperature of the feed liquid in the kettle body is lower than the preset temperature, the temperature regulating device heats the feed liquid in the kettle body; when the temperature of the feed liquid in the kettle body is higher than the preset temperature, the temperature adjusting device cools the feed liquid in the kettle body. The reaction kettle coated with the microspheres can monitor and accurately control the reaction conditions in the kettle body in the process of coating the particles, reduces manual operation, and is favorable for improving the product quality.
Description
Technical Field
The utility model relates to the field of microsphere coating production, in particular to a microsphere coating reaction kettle.
Background
In the process of an immunodiagnostic kit using microspheres as raw materials, coating of antibodies or antigens on the surface of the microspheres is an extremely important step. The small-batch coating is generally carried out in a beaker, the large-batch coating is generally carried out in a feed liquid barrel, the process is complex, more manual operations are involved in the conventional process, and the problems of insufficient mixing uniformity and the like can be caused by the lack of monitoring and adjusting capabilities of conditions such as stirring strength, temperature, pH value, ionic strength and the like. The manual operation easily brings errors, so that batch-to-batch differences exist among different batches of kits, and even the quality of products is unqualified.
Along with scale enlargement, the larger the volume of the coating material liquid is, the larger the operation difficulty is, the larger the influence caused by the error of manual operation is, and the stable enlargement of the production scale and the control of the batch-to-batch difference are not facilitated. The operational convenience problems of a series of process flows of liquid material transfer, coating, liquid change, dispersion and the like caused by the enlargement of the coating scale of the microspheres are to be solved.
SUMMERY OF THE UTILITY MODEL
The utility model provides a microsphere-coated reaction kettle, which is used for solving at least one technical problem.
The utility model relates to a microsphere-coated reaction kettle, which comprises: a kettle body, a kettle cover, a stirrer, a motor, a parameter monitoring component and a temperature adjusting device,
wherein the kettle cover is arranged at the top of the kettle body, the stirrer is arranged inside the kettle body, the motor is arranged on the kettle cover and connected with the stirrer,
the parameter monitoring assembly comprises a temperature sensor, and the temperature sensor is inserted into the kettle body from the kettle cover to monitor the temperature of the feed liquid in the kettle body;
when the temperature of the feed liquid in the kettle body is lower than a preset temperature, the temperature regulating device heats the feed liquid in the kettle body; when the temperature of the feed liquid in the kettle body is higher than the preset temperature, the temperature adjusting device cools the feed liquid in the kettle body.
In one embodiment, the parameter monitoring assembly further comprises: and the PH electrode is inserted into the kettle body from the kettle cover so as to monitor the PH value of the feed liquid in the kettle body.
In one embodiment, the parameter monitoring assembly further comprises: and the conductivity electrode is inserted into the kettle body from the kettle cover so as to detect the conductivity of the feed liquid in the kettle body.
In one embodiment, the temperature adjustment device includes: the heating base, the cauldron body sets up on the heating base, when the temperature of the internal portion's of cauldron feed liquid is less than preset temperature, the heating base is right the internal portion's of cauldron feed liquid heats.
In one embodiment, the temperature adjustment device further comprises: the cooling tube, the cooling tube sets up the inside of the cauldron body, when the temperature of the internal portion's of cauldron feed liquid was higher than preset temperature, let in the cooling tube coolant liquid in order to right the internal portion's of cauldron feed liquid cools down.
In one embodiment, a cooling liquid inlet and a cooling liquid outlet are formed in the kettle cover, one end of the cooling pipe is connected with the cooling liquid inlet, and the other end of the cooling pipe extends downwards to a position close to the bottom of the kettle body and then bends to extend upwards to the cooling liquid outlet and is connected with the cooling liquid outlet.
In one embodiment, at least a portion of the cooling tube extends in a spiral or wave shape.
In one embodiment, at least one set of symmetrically arranged flow baffle plates are arranged on the inner wall of the kettle body, and the flow baffle plates extend along the height direction of the kettle body.
In one embodiment, the kettle cover is provided with at least two feed liquid inlets; and a material liquid outlet valve is arranged on the side wall of the kettle body close to the bottom.
In one embodiment, the kettle body is configured as a cylinder or sphere.
Compared with the prior art, the utility model has the advantages that: it is rotatory to drive the agitator through the motor, make the stirring more even, can monitor the internal reaction condition of cauldron through setting up parameter monitoring subassembly, wherein, through the temperature of the inside feed liquid of temperature sensor detectable reaction process reation kettle, when the temperature of the internal portion of cauldron feed liquid is less than when predetermineeing the temperature, temperature regulation apparatus heats the cauldron body and inside feed liquid, when the temperature of the internal portion of cauldron feed liquid is higher than when predetermineeing the temperature, temperature regulation apparatus cools down the internal portion of cauldron feed liquid, thereby realized accurate control particle peridium in-process temperature, manual operation has been reduced, be favorable to improving product quality, and make things convenient for follow-up liquid changing, operation such as dispersion, and then be favorable to enlarging the productivity of external diagnosis product (IVD) enterprise.
Drawings
The utility model will be described in more detail hereinafter on the basis of embodiments and with reference to the accompanying drawings.
FIG. 1 is a schematic view of the structure of a reaction vessel in one embodiment of the present invention;
FIG. 2 is a schematic view of the structure of a reaction vessel in another embodiment of the present invention;
fig. 3 is a schematic structural view of a baffle plate according to an embodiment of the present invention.
Reference numerals:
1-kettle body; 2-kettle cover; 3, a motor; 4-a stirrer; 5-heating the base;
6-a cooling pipe; 7-a temperature sensor; 8-PH electrode; 9-conductivity electrodes; 10-coolant inlet;
11-coolant outlet; 12-a flow baffle; 13-feed liquid inlet; 14-feed liquid outlet valve.
Detailed Description
The utility model will be further explained with reference to the drawings.
As shown in fig. 1 to 3, the present invention provides a microsphere-coated reaction vessel comprising: the device comprises a kettle body 1, a kettle cover 2, a stirrer 4, a motor 3, a parameter monitoring assembly and a temperature adjusting device. The parameter monitoring assembly comprises a temperature sensor 7, and the temperature sensor 7 is inserted into the kettle body 1 from the kettle cover 2 to monitor the temperature of the feed liquid in the kettle body 1; when the temperature of the feed liquid in the kettle body 1 is lower than the preset temperature, the temperature regulating device heats the feed liquid in the kettle body 1; when the temperature of the feed liquid in the kettle body 1 is higher than the preset temperature, the temperature adjusting device cools the feed liquid in the kettle body 1.
In the utility model, the stirrer 4 is driven to rotate by the motor 3, so that the stirring is more uniform, the reaction condition in the kettle body 1 can be monitored by setting the parameter monitoring assembly, wherein the temperature of the material liquid in the reaction kettle in the reaction process can be detected by the temperature sensor 7, when the temperature of the material liquid in the kettle body 1 is lower than the preset temperature, the temperature regulating device heats the kettle body 1 and the material liquid in the kettle body 1, and when the temperature of the material liquid in the kettle body 1 is higher than the preset temperature, the temperature regulating device cools the material liquid in the kettle body 1, so that the temperature in the particle coating process is accurately controlled, the manual operation is reduced, the product quality is improved, the subsequent operations of liquid changing, dispersing and the like are facilitated, and the capacity of in-vitro diagnosis product (IVD) enterprises is further facilitated to be enlarged.
In one embodiment, the parameter monitoring component further comprises: PH electrodes 8 and/or conductivity electrodes 9. Wherein, the PH electrode 8 is inserted into the kettle body 1 from the kettle cover 2 to detect the PH value of the feed liquid in the kettle body 1; a conductivity electrode 9 is inserted into the inside of the vessel body 1 from the vessel cover 2 to detect the conductivity of the feed liquid inside the vessel body 1.
In this embodiment, the PH and the conductivity of the feed liquid during the reaction process can be respectively detected in real time by the PH electrode 8 and the conductivity electrode 9, and when the PH and/or the conductivity do not meet the process requirements, the PH and/or the conductivity can be adjusted by adding the corresponding feed liquid into the kettle body 1.
Specifically, at least three through holes are formed in the kettle cover 2, the temperature sensor 7, the PH electrode 8 and the conductivity electrode 9 are respectively inserted into the kettle body 1 from the corresponding through holes, and sealing gaskets are arranged between the temperature sensor 7, the PH electrode 8 and the conductivity electrode 9 and the hole walls of the corresponding through holes, so that the airtightness inside the kettle body 1 is ensured.
Further, the parameter monitoring assembly further comprises: a pressure sensor. The pressure sensor is inserted into the kettle body 1 from the through hole on the kettle cover 2, and a sealing gasket is arranged between the pressure sensor and the wall of the through hole, so that the pressure inside the kettle body 1 can be detected in the reaction process.
In one embodiment, the temperature regulating device comprises a cooling tube 6 and a heating base 5.
Wherein, cauldron body 1 sets up on heating base 5, and when the temperature of the inside feed liquid of cauldron body 1 was less than preset temperature, heating base 5 heated the inside feed liquid of cauldron body 1.
The cooling tube 6 sets up in the inside of the cauldron body 1, and when the temperature of the inside feed liquid of the cauldron body 1 was higher than preset temperature, let in the coolant liquid in the cooling tube 6 and cool down with the feed liquid to the cauldron body 1 inside.
Preferably, the kettle cover 2 is provided with a cooling liquid inlet 10 and a cooling liquid outlet 11, one end of the cooling pipe 6 is connected with the cooling liquid inlet 10, and the other end of the cooling pipe 6 extends downwards to a position close to the bottom of the kettle body 1 and then bends to extend upwards to the cooling liquid outlet 11 and is connected with the cooling liquid outlet.
In other words, the two ends of the cooling pipe 6 are respectively the cooling liquid inlet 10 and the cooling liquid outlet 11, and in the reaction process, when the temperature inside the kettle body 1 is too high, the cooling liquid is injected into the cooling pipe 6 from the cooling liquid inlet 10, and the cooling liquid exchanges heat with the feed liquid in the reaction kettle and flows out from the cooling liquid outlet 11, so that the purpose of reducing the temperature of the feed liquid is realized. Meanwhile, the cooling pipe 6 extends from the top of the kettle body 1 to a position close to the bottom of the kettle body 1 in the kettle body 1, so that the material liquid in the kettle body 1 can be fully cooled.
It is further preferred that at least a part of the cooling tube 6 extends in a spiral or wave-like manner. This makes it possible to increase the length of the cooling pipe 6 and the surface area of the cooling pipe 6, thereby further improving the cooling effect.
Among them, the spiral cooling pipe 6 has a larger surface area than the wavy cooling pipe 6, and the cooling efficiency is higher, but the number of dead corners is also large, and the cleaning is difficult, and the fouling material is easily caused. When the capacity of the kettle body 1 is large and the requirement on cooling efficiency is high, a spiral cooling pipe 6 can be adopted; conversely, a wavy cooling tube 6 may be used.
In the present embodiment, the cooling liquid is provided by a cooling device, and the cooling device is respectively connected to the cooling liquid inlet 10 and the cooling liquid outlet 11 to introduce the cooling liquid into the cooling pipe 6. The cooling liquid is generally cooling water, and correspondingly, the cooling device is a water chiller.
In one embodiment, at least two feed liquid inlets 13 are provided on the kettle cover 2, and a feed liquid outlet valve 14 is provided on the side wall of the kettle body 1 near the bottom.
Wherein, can add many different feed liquids to the cauldron body 1 through a plurality of feed liquid inlets 13, feed liquid outlet valve 14 sets up on the lateral wall of cauldron body 1 near the bottom, can conveniently and fully discharge the feed liquid in the cauldron body 1.
Specifically, the automatic feeding adjustment can be realized by connecting the corresponding feeding device with the feed liquid inlet 13 to inject the feed liquid into the kettle body 1. For example, when the pH and/or conductivity do not meet the process requirements during the reaction, the corresponding feed liquid can be automatically added into the kettle body 1 through the corresponding feeding device for adjustment.
In one embodiment, at least one set of symmetrically arranged flow baffle plates 12 are arranged on the inner wall of the kettle body 1, and the flow baffle plates 12 extend along the height direction of the kettle body 1.
Due to the existence of the flow baffle plate 12, a great impact is generated on the material liquid, the turbulence in the kettle body 1 can be increased, and the sufficient mixing of the material liquid is facilitated. In a similar way, the cooling pipe 6 is arranged in the kettle body 1, so that the feed liquid is stirred more uniformly.
In one embodiment, the agitator 4 comprises: the stirring shaft is arranged on the central shaft of the kettle body 1, the upper end of the stirring shaft extends out of the kettle cover 2 and is connected with the motor 3, and the lower end of the stirring shaft extends downwards to a position close to the bottom of the kettle body 1; and at least two groups of stirring blades are sequentially arranged on the stirring shaft at intervals from bottom to top.
Wherein, each group of stirring blades can be paddle type, propelling type, turbine type, anchor type or frame type.
In one embodiment, as shown in fig. 1 and 2, the vessel body 1 is configured in a cylindrical or spherical shape.
Specifically, the side of the kettle body 1 is provided with an observation window, so that an operator can observe the reaction state of the feed liquid in the kettle body 1.
It should be noted that, each device of the present invention can be controlled by a control system (e.g., a central control computer), so as to realize automatic production. For example, the control system can control the rotation speed of the motor 3, the heating temperature of the heating base 5, the injection of the cooling liquid into the cooling pipe 6 by the cooling device, the injection of the feed liquid into the feeding device from the feed liquid inlet 13, and the like.
The specific process for the scale-up production of a batch of latex reagents (batch 2L) using the microsphere-coated reactor of the present invention will now be described.
a) and starting power supplies of the reaction kettle, the central control computer and the water cooler, and setting the temperature of the water cooler in advance for precooling.
b) The latex microsphere suspension is weighed and added into the reaction kettle from the feed liquid inlet 13, and then the activation buffer is added from the feed liquid inlet 13.
c) A PH electrode 8, a conductivity electrode 9, a temperature sensor 7 and a pressure sensor are arranged on the kettle cover 2, a water outlet and a water return port of the water cooler are respectively communicated with a cooling liquid inlet 10 and a cooling liquid outlet 11 of the reaction kettle through pipelines, and the operation parameters of the reaction kettle are set in a central control computer. And starting the motor 3 to drive the stirrer 4 to start stirring and uniformly mixing the material liquid, and monitoring reaction conditions of the material liquid in the reaction kettle, such as pH value, conductivity, temperature and the like.
d) The EDC solution was slowly added to the reaction vessel through the feed inlet 13.
e) The reaction kettle is sealed, and the time is timed for 30 min. And monitoring the pH value, the conductivity and the temperature in the reaction kettle, and if abnormity occurs, adding corresponding feed liquid to adjust or adjust the heating temperature of the heating base 5 or introducing cooling water.
a) after activation of the microspheres is completed, a coupling buffer solution is added into the reaction kettle from the feed liquid inlet 13, the motor 3 is started to drive the stirrer 4 to stir the feed liquid, and reaction conditions such as pH value, conductivity and temperature in the reaction kettle are monitored.
b) The antibody was slowly added to the reaction vessel through the feed inlet 13.
c) The reaction kettle is sealed, and the time is counted for 16 h. And monitoring the pH value, the conductivity and the temperature of the feed liquid in the reaction kettle, and if abnormity occurs, adding the corresponding feed liquid to adjust or adjust the heating temperature of the heating base 5 or introducing cooling water.
a) and after the coupling of the microspheres is finished, connecting a microsphere ultrafiltration system with the reaction kettle, wherein an inlet of the microsphere ultrafiltration system is connected with a feed liquid outlet valve 14 of the reaction kettle, a return pipe of the microsphere ultrafiltration system is connected with one feed liquid inlet 13 of the reaction kettle, and a liquid supplementing pipe of the microsphere ultrafiltration system is connected with the other feed liquid inlet 13. And monitoring the pressure in the reaction kettle to ensure that the reaction kettle is sealed and keeps a negative pressure state. And (4) carrying out ultrafiltration liquid change according to the existing ultrafiltration process operating rules. The volume of the liquid change is based on the condition that the real-time pH value and the electric conductivity in the reaction kettle meet the process requirements.
b) Weighing the protein protective agent and the preservative according to the process requirements, adding the protein protective agent and the preservative into the reaction kettle from a feed liquid inlet 13, and starting the motor 3 to drive the stirrer 4 to stir the feed liquid until the protein protective agent and the preservative are completely dissolved.
c) The stirring was stopped, the feed liquid outlet valve 14 was opened, and the antibody-coated latex microsphere solution was completely discharged. And (4) taking ultrafiltrate to flush the reaction kettle, and collecting the ultrafiltrate for later use.
homogenizing and dispersing according to the existing technological operating procedures. Finally, the volume is replenished to 2L by using a preservation solution.
And 6, detection and evaluation:
and taking a small sample of the reagent, and detecting the performance of the reagent on a computer according to a quality inspection rule to evaluate the production effect.
While the utility model has been described with reference to a preferred embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the utility model. In particular, the technical features mentioned in the embodiments can be combined in any way as long as there is no structural conflict. It is intended that the utility model not be limited to the particular embodiments disclosed, but that the utility model will include all embodiments falling within the scope of the appended claims.
Claims (10)
1. A microsphere-coated reaction kettle is characterized by comprising: a kettle body, a kettle cover, a stirrer, a motor, a parameter monitoring component and a temperature adjusting device,
wherein the kettle cover is arranged at the top of the kettle body, the stirrer is arranged inside the kettle body, the motor is arranged on the kettle cover and connected with the stirrer,
the parameter monitoring assembly comprises a temperature sensor, and the temperature sensor is inserted into the kettle body from the kettle cover to monitor the temperature of the feed liquid in the kettle body;
when the temperature of the feed liquid in the kettle body is lower than a preset temperature, the temperature regulating device heats the feed liquid in the kettle body; when the temperature of the feed liquid in the kettle body is higher than the preset temperature, the temperature adjusting device cools the feed liquid in the kettle body.
2. The microsphere coated reactor of claim 1, wherein the parameter monitoring assembly further comprises: and the pH electrode is inserted into the kettle body from the kettle cover so as to monitor the pH value of the feed liquid in the kettle body.
3. The microsphere coated reactor of claim 2, wherein the parameter monitoring assembly further comprises: and the conductivity electrode is inserted into the kettle body from the kettle cover so as to detect the conductivity of the feed liquid in the kettle body.
4. A microsphere coated reactor according to any one of claims 1 to 3 wherein the temperature regulating means comprises: the heating base, the cauldron body sets up on the heating base, when the temperature of the internal portion's of cauldron feed liquid is less than preset temperature, the heating base is right the internal portion's of cauldron feed liquid heats.
5. The microsphere-coated reactor according to claim 4, wherein the temperature regulating device further comprises: the cooling tube, the cooling tube sets up the inside of the cauldron body, when the temperature of the internal portion's of cauldron feed liquid was higher than preset temperature, let in the cooling tube coolant liquid in order to right the internal portion's of cauldron feed liquid cools down.
6. A microsphere-coated reaction kettle according to claim 5, wherein a cooling liquid inlet and a cooling liquid outlet are arranged on the kettle cover, one end of the cooling pipe is connected with the cooling liquid inlet, and the other end of the cooling pipe extends downwards to be close to the bottom of the kettle body and then bends to extend upwards to the cooling liquid outlet and is connected with the cooling liquid outlet.
7. A microsphere-coated reactor as claimed in claim 6 wherein at least a portion of the cooling tube extends in a spiral or wave shape.
8. The reactor of any one of claims 1-3, wherein at least one set of flow baffles is symmetrically disposed on the inner wall of the reactor body, and the flow baffles extend along the height direction of the reactor body.
9. A reaction kettle coated with microspheres according to any one of claims 1-3, wherein the kettle cover is provided with at least two feed liquid inlets; and a material liquid outlet valve is arranged on the side wall of the kettle body close to the bottom.
10. A microsphere coated reactor according to any one of claims 1 to 3, wherein the reactor body is configured in a cylindrical or spherical shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122067608.4U CN216260715U (en) | 2021-08-30 | 2021-08-30 | Microsphere coating reaction kettle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122067608.4U CN216260715U (en) | 2021-08-30 | 2021-08-30 | Microsphere coating reaction kettle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216260715U true CN216260715U (en) | 2022-04-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122067608.4U Active CN216260715U (en) | 2021-08-30 | 2021-08-30 | Microsphere coating reaction kettle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216260715U (en) |
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2021
- 2021-08-30 CN CN202122067608.4U patent/CN216260715U/en active Active
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