CN216233584U

CN216233584U - PCR array kit for gene expression difference screening

Info

Publication number: CN216233584U
Application number: CN202121603363.6U
Authority: CN
Inventors: 陈美娟; 陈成成; 陈冲; 崔佳佳; 曹琴琴; 杨敏; 陆梦娇; 王振球; 章进军; 贾俊才
Original assignee: Shanghai Woji Gene Technology Co ltd
Current assignee: Shanghai Woji Gene Technology Co ltd
Priority date: 2021-07-14
Filing date: 2021-07-14
Publication date: 2022-04-08
Anticipated expiration: 2031-07-14

Abstract

The utility model discloses a PCR array kit for gene expression difference screening, which belongs to the technical field of kits and is provided with a 96-well plate, the kit also comprises a kit body, a plurality of reagent pipe slots are arranged on the kit body and correspondingly arranged in the 96-well plate, and the detection is convenient; all through folded piece fixedly connected with wooden plug on every reagent tube seat, the bottom of every reagent tube seat all is equipped with the blotter, and is equipped with the heat-conducting layer on the outer wall, and the outer wall of heat-conducting layer is equipped with the rubber pad, and the outer wall that drives the rubber pad is equipped with the heat preservation, and the winding has water cooling plant on the heat preservation, all is equipped with the filler between two liang of reagent tube seats, all is equipped with digital code on the horizontal and the vertical of kit body, and the parcel has one deck aluminium foil plastic envelope bag on the kit body. When the reagent tube is placed in the reagent box, the reagent tube is not easy to be damaged by external force, and for some reagents needing low-temperature storage, the temperature of the reagent box can be kept stable well, the refrigeration storage effect is improved, and the quality of the reagents is improved.

Description

PCR array kit for gene expression difference screening

Technical Field

The utility model relates to the technical field of kits, in particular to a PCR array kit for gene expression difference screening.

Background

The real-time quantitative fluorescence PCR realizes quantitative and qualitative analysis of the initial template by detecting the fluorescence signal corresponding to each cycle amplification product of the PCR in real time.

In the prior art, some reagent boxes are poor in sealing performance and easy to volatilize and mix reagents, reagent tubes are placed in the reagent boxes and are easy to be damaged by external force, and for some reagents needing low-temperature preservation, the temperature of the reagent boxes cannot be kept stable well, and reagent effect is influenced.

Based on this, the utility model designs a PCR array kit for gene expression difference screening to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a PCR array kit for gene expression difference screening, which solves the problems that some kits provided in the background art have poor tightness, are easy to volatilize and mix reagents, are easy to damage by external force when reagent tubes are put into the kits, are inconvenient to search the reagents, cannot well keep the temperature of the kits stable for some reagents needing low-temperature storage, and influence the reagent effect.

In order to achieve the purpose, the utility model provides the following technical scheme: a PCR array kit for screening gene expression difference is provided with a 96-pore plate, and further comprises a kit body, wherein a plurality of reagent tube grooves are arranged on the kit body and correspondingly arranged in the 96-pore plate, a wooden plug is fixedly connected on each reagent tube groove through a folding piece, the bottom of each reagent tube groove is provided with a buffer cushion, the outer wall of each reagent tube groove is provided with a heat conduction layer, the outer wall of each heat conduction layer is provided with a rubber cushion, the outer wall of each rubber cushion is provided with a heat insulation layer, the heat insulation layer is wound with a water cooling device, and fillers are arranged between every two reagent tube grooves, so that the tightness of the kit is improved, the reagent is not easy to volatilize and mix, the reagent tubes are not easy to be damaged by external force when being put into the kit, and the temperature of the kit can be well kept stable for some reagents needing low-temperature preservation, and the refrigeration preservation effect is improved, improve the quality of the reagent.

As a further scheme of the utility model, the water cooling device comprises a condensation pipe, the condensation pipe is wound on the outer wall of the reagent pipe groove, and a refrigerator and a circulating water pump are arranged on the condensation pipe.

As a further scheme of the utility model, a control box is arranged on one outer wall of the kit body, a display screen, a control button and a storage battery are arranged in the control box, and the control box is electrically connected with the refrigerator and the circulating water pump through the storage battery.

As a further scheme of the utility model, the kit body is provided with digital codes in the transverse direction and the longitudinal direction, so that people can conveniently search for reagents, and the time is saved.

As a further aspect of the present invention, a plurality of heat conducting fins are disposed in the heat conducting layer.

As a further scheme of the utility model, the filler is sponge filler, and a temperature sensor is arranged in the filler.

As a further scheme of the utility model, the kit body is wrapped with a layer of aluminum foil plastic packaging bag.

Compared with the prior art, the utility model has the beneficial effects that:

according to the utility model, the wood plug and the aluminum foil plastic packaging bag are arranged, so that the sealing property is improved, the volatilization and mixing of the reagents are prevented, the stable safety of reagent storage is improved by arranging the buffer cushion, the rubber pad and the sponge filler, the reagent tube is not easily damaged by external force when being put into the reagent kit, and the heat preservation layer and the water cooling device are arranged, so that the temperature of the reagent kit can be well kept stable for some reagents needing low-temperature storage, the refrigeration storage effect is improved, and the quality of the reagents is improved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic diagram of the overall structure of the present invention;

fig. 2 is a cross-sectional view of the present invention.

In the drawings, the components represented by the respective reference numerals are listed below:

1. a cartridge body; 2. a reagent tube slot; 3. a folding member; 4. a wooden plug; 5. a cushion pad; 6. a heat conductive layer; 7. a rubber pad; 8. a heat-insulating layer; 9. a water cooling device; 10. a filler; 11. a condenser tube; 12. a refrigerator; 13. a water circulating pump; 14. a control box; 15. digital coding; 16. a temperature sensor; 17. 96-well plates.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-2, the present invention provides a technical solution: a PCR array kit for gene expression difference screening is provided with a 96-pore plate 17 and further comprises a kit body 1, wherein digital codes 15 are arranged on the kit body 1 in the transverse direction and the longitudinal direction, people can conveniently find reagents, time is saved, a plurality of reagent pipe grooves 2 are arranged on the kit body 1 and correspondingly installed in the 96-pore plate 17, namely, a vertical reagent pipe groove 2 is respectively and downwards arranged in each hole of the 96-pore plate 17, and the diameter of an opening at the top end of each reagent pipe groove 2 is matched with the diameter of the hole in the 96-pore plate 17. Every all through 3 fixedly connected with wooden plugs 4 of folded piece, every on the reagent tube seat 2 the bottom of reagent tube seat 2 all is provided with blotter 5, and is provided with heat-conducting layer 6 on the outer wall, the outer wall of heat-conducting layer 6 is provided with rubber pad 7, the outer wall of rubber pad 7 is provided with heat preservation 8, the winding has water cooling plant 9 on the heat preservation 8, two liang all be provided with filler 10 between the reagent tube seat 2, water cooling plant 9 includes condenser pipe 11, the winding of condenser pipe 11 is at the outer wall of reagent tube seat 2, be provided with refrigerator 12 and circulating water pump 13 on the condenser pipe 11, the parcel has one deck aluminium foil plastic envelope bag on the kit body 1.

Through setting up wooden plug 4 and aluminium foil plastic envelope bag, improved the leakproofness, prevent that reagent from volatilizing and mixing, through setting up blotter 5, rubber pad 7 and sponge filler 10, improved the stable security that reagent was deposited, be difficult for receiving external force when reagent pipe puts into the kit and damage, through setting up heat preservation 8 and water cooling plant 9, to some reagents that need the low temperature save, messenger's kit temperature that can be fine remains stable, improves the refrigeration and preserves the effect, improves the reagent quality.

The working principle is as follows: above-mentioned PCR array kit during operation for gene expression difference screening, at first, the staff at first places the reagent pipe that is equipped with the reagent in reagent pipe groove 2 according to digital code 15 according to the description, cover wooden plug 4, when needing the refrigeration, open refrigerator 12 and circulating water pump 13 through the control box, the cooling water circulates in condenser pipe 11 and refrigerates the reagent, wooden plug 4 and aluminium foil plastic envelope, the leakproofness has been improved, prevent the volatile mixture of reagent, blotter 5, rubber pad 7 and sponge filler 10, the stable security of reagent deposit has been improved, be difficult for receiving external force when the reagent pipe puts into the kit and damage.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the utility model disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the utility model to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the utility model and the practical application, to thereby enable others skilled in the art to best utilize the utility model. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A PCR array kit for differential screening of gene expression, equipped with a 96-well plate (17), characterized in that: the kit further comprises a kit body (1), wherein a plurality of reagent pipe grooves (2) are formed in the kit body (1), the reagent pipe grooves are correspondingly installed in a 96-hole plate (17), each reagent pipe groove (2) is fixedly connected with a wooden plug (4) through a folding piece (3), each reagent pipe groove (2) is provided with a cushion pad (5) at the bottom and a heat conduction layer (6) on the outer wall, the outer wall of the heat conduction layer (6) is provided with a rubber pad (7), the outer wall of the rubber pad (7) is provided with a heat insulation layer (8), a water cooling device (9) is wound on the heat insulation layer (8), and fillers (10) are arranged between every two reagent pipe grooves (2).

2. The PCR array kit for differential screening of gene expression according to claim 1, wherein: the water cooling device (9) comprises a condensation pipe (11), the condensation pipe (11) is wound on the outer wall of the reagent pipe groove (2), and a refrigerator (12) and a circulating water pump (13) are arranged on the condensation pipe (11).

3. The PCR array kit for differential screening of gene expression according to claim 2, wherein: the kit comprises a kit body (1) and is characterized in that a control box (14) is arranged on one outer wall of the kit body (1), a display screen, a control button and a storage battery are arranged in the control box (14), and the control box (14) is electrically connected with a refrigerator (12) and a circulating water pump (13) through the storage battery.

4. The PCR array kit for differential screening of gene expression according to claim 1, wherein: the kit body (1) is provided with digital codes (15) in the transverse direction and the longitudinal direction.

5. The PCR array kit for differential screening of gene expression according to claim 1, wherein: a plurality of heat conducting fins are arranged in the heat conducting layer (6).

6. The PCR array kit for differential screening of gene expression according to claim 1, wherein: the filler (10) is a sponge filler, and a temperature sensor (16) is arranged in the filler (10).

7. The PCR array kit for differential screening of gene expression according to claim 1, wherein: the kit body (1) is wrapped with a layer of aluminum foil plastic packaging bag.