CN215924918U

CN215924918U - Semi-permeable membrane filtering and separating device for preparing single-cell suspension

Info

Publication number: CN215924918U
Application number: CN202122438905.5U
Authority: CN
Inventors: 刘杰; 王盼飞; 刘静
Original assignee: Tianjin Baiao Pharmaceutical Technology Co ltd
Current assignee: Tianjin Baiao Pharmaceutical Technology Co ltd
Priority date: 2021-10-11
Filing date: 2021-10-11
Publication date: 2022-03-01
Anticipated expiration: 2031-10-11

Abstract

The utility model discloses a semipermeable membrane filtering and separating device for preparing single cell suspension, which relates to the technical field of cell separation and comprises a working box, wherein a box cover is hinged on the working box, a rotating cylinder is rotatably connected in the working box, an installation cylinder is slidably arranged in the rotating cylinder, a locking mechanism is arranged between the installation cylinder and the rotating cylinder, the locking mechanism has a locking effect on the installation cylinder, a filter cylinder is slidably arranged in the installation cylinder, a semipermeable membrane is arranged at the bottom of the filter cylinder, and a driving motor is arranged in the working box and used for driving the rotating cylinder to rotate. Through the cooperation between the work box, a rotation section of thick bamboo, an installation section of thick bamboo, cartridge filter, pellicle, driving motor drives and rotates a section of thick bamboo and rotates, drives the high-speed rotation of cartridge filter through an installation section of thick bamboo, through pellicle with cell and liquid separation, easy operation is convenient to improve this pellicle filtration separation device's that preparation unicellular suspension was used practicality.

Description

Semi-permeable membrane filtering and separating device for preparing single-cell suspension

Technical Field

The utility model relates to the technical field of cell separation, in particular to a semipermeable membrane filtering and separating device for preparing single-cell suspension.

Background

At present, many tissues and organs of adult animals, such as epidermis and hematopoietic system, have the capability of repairing and regenerating, adult stem cells play a key role in the repair and regeneration, under specific conditions, the adult stem cells either generate new stem cells or differentiate according to a certain program to form new functional cells, so that the tissues and organs keep the dynamic balance of growth and recession, in the process of separating the stem cells, the prior art adopts a shaking and stirring mode to realize the separation through stem cell stratification.

The pellicle that present preparation single cell suspension was used filters separator, like the chinese patent of CN201921377084.5 name "a tissue separator who prepares single cell suspension", discloses a tissue separator who prepares single cell suspension, belongs to the tissue separation field, including cup, cutter arbor and the blade of connection in the cutter arbor bottom, the blade is located the cup, and cutter arbor upper portion stretches out from cup open-top, the location is provided with the cell screen in the cup, and this cell screen divides into the cutting chamber of top and the suspension chamber that is located the below with cup inside, and the sieve mesh intercommunication cutting chamber and the suspension chamber of cell screen, cell screen are provided with the cutting edge corresponding to the border position of every sieve mesh, the blade is located cutting intracavity and blade lower extreme face butt cutting edge up end. The utility model firstly utilizes the continuous cutting action between the blade on the cell screen and the rotating blade to thoroughly separate the sample, and then the suspension falls into the suspension cavity for direct utilization through the filtration of the screen holes, and the separation and filtration functions are completed in one separator.

The defects of the prior art are as follows: traditional tissue separator places the test tube of unicellular suspension through high-speed rotation, utilizes centrifugal force to realize the purpose of tissue separation, prior art's separation only can realize organizing and the layering of liquid part, still need rely on the manpower to pour out cell and parting liquid after the separation at the in-process of separation, can cause the mixture again at the in-process of empting, lead to the separation incomplete.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a semipermeable membrane filtration separation device for preparing single cell suspension, which aims to overcome the defects that the separation device in the prior art has low working efficiency and the fixation of a test tube cannot be adjusted.

In order to achieve the above purpose, the utility model provides the following technical scheme: the utility model provides a pellicle filtration separation device that preparation unicellular suspension was used, includes the work box, it has a case lid to articulate on the work box, it is connected with a rotation section of thick bamboo to rotate in the work box, it is provided with an installation section of thick bamboo to slide in the rotation section of thick bamboo, be provided with a locking mechanism between an installation section of thick bamboo and the rotation section of thick bamboo, locking mechanism plays the locking effect to an installation section of thick bamboo, it is provided with a cartridge filter to slide in the installation section of thick bamboo, the cartridge filter bottom is provided with the pellicle, be provided with a driving motor in the work box, driving motor is used for the drive to rotate a section of thick bamboo and rotates.

Further, the mounting groove has been seted up to the bottom of the case, driving motor installs inside the mounting groove, a dwang of fixedly connected with in the driving motor axis of rotation, the dwang rotates to be connected in the bottom of the case, driving motor's one end and a rotation section of thick bamboo fixed connection are kept away from to the dwang.

Furthermore, an annular groove is formed in the installation cylinder, and the semipermeable membrane is installed on the installation cylinder through the annular groove.

Further, locking mechanism includes the spout, the spout is seted up on rotating the section of thick bamboo, it is provided with a joint pole to slide in the spout, be provided with the spring between joint pole and the spout, the joint groove has been seted up on the installation section of thick bamboo, the elasticity of spring orders about joint pole joint and advances the joint inslot, the vertical spacing groove of having seted up on the lateral wall of installation section of thick bamboo, the vertical upwards slip setting of joint pole is at the spacing inslot.

Furthermore, a limiting block is arranged in the rotating cylinder, and the mounting cylinder is in butt fit with the limiting block.

Further, be provided with the spacing ring in the installation section of thick bamboo, the cartridge filter and spacing ring butt, the liquid outlet has been seted up to the installation section of thick bamboo bottom.

Further, the installation cylinder is rotatably connected with a sealing cover, the sealing cover is provided with a buckle, the installation cylinder is provided with a clamping groove, the buckle is matched with the clamping groove in a clamping manner, and the sealing cover is provided with an operation portion.

Compared with the prior art, the utility model has the beneficial effects that: through the cooperation between the work box, a rotation section of thick bamboo, an installation section of thick bamboo, cartridge filter, pellicle, driving motor drives and rotates a section of thick bamboo and rotate, and then drives the high-speed rotation of cartridge filter through an installation section of thick bamboo to with cell and liquid separation through the pellicle, easy operation is convenient, the also very thorough of separation, thereby improve this pellicle filtration separation device that preparation unicellular suspension was used's practicality.

Drawings

In order to more clearly illustrate the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to the drawings.

FIG. 1 is a schematic diagram of an overall structure provided in an embodiment of the present invention;

FIG. 2 is a schematic cross-sectional view of an embodiment of the present invention;

FIG. 3 is a schematic structural diagram of a connection mode between a driving motor and a rotary drum according to an embodiment of the present invention;

FIG. 4 is a schematic view of an internal structure of a rotary drum according to an embodiment of the present invention;

FIG. 5 is a schematic view of an external structure of a mounting barrel according to an embodiment of the present invention;

FIG. 6 is a schematic cross-sectional structural view of a filter cartridge according to an embodiment of the present invention;

fig. 7 is a schematic structural diagram of a filter cartridge according to an embodiment of the present invention.

Description of reference numerals: 1. a work box; 101. a box cover; 2. a drive motor; 201. rotating the rod; 3. mounting grooves; 4. a rotating cylinder; 401. a limiting block; 5. mounting the cylinder; 6. a locking mechanism; 601. a chute; 602. a spring; 603. a clamping and connecting rod; 604. a clamping groove; 605. a limiting groove; 7. a filter cartridge; 701. an annular groove; 8. a semi-permeable membrane; 9. a sealing cover; 901. buckling; 902. a card slot; 10. an operation section; 11. a limiting ring; 12. and a liquid outlet.

Detailed Description

In order to make the technical solutions of the present invention better understood, those skilled in the art will now describe the present invention in further detail with reference to the accompanying drawings.

Referring to fig. 1-7, a semipermeable membrane filtration and separation device for preparing a single cell suspension according to an embodiment of the present invention includes a working box 1, a box cover 101 is hinged on the working box 1, a rotating cylinder 4 is rotatably connected in the working box 1, an installation cylinder 5 is slidably disposed in the rotating cylinder 4, a locking mechanism 6 is disposed between the installation cylinder 5 and the rotating cylinder 4, the locking mechanism 6 locks the installation cylinder 5, a filter cylinder 7 is slidably disposed in the installation cylinder 5, a semipermeable membrane 8 is disposed at the bottom of the filter cylinder 7, a driving motor 2 is disposed in the working box 1, and the driving motor 2 is used for driving the rotating cylinder 4 to rotate.

Specifically, the semipermeable membrane filtering and separating device for preparing the single cell suspension comprises a working box 1, wherein a box cover 101 is hinged on the working box 1, the box cover 101 is covered when the working box 1 works or after the working is finished, so that the function of protection is achieved, dust can fall into the working box 1, and the cleaning is not convenient; the cell separation device is characterized in that a rotating cylinder 4 is rotatably connected inside the working box 1, an installation cylinder 5 is slidably arranged inside the rotating cylinder 4, the rotating cylinder 4 rotates to drive the installation cylinder 5 to rotate, a filter cylinder 7 inside the installation cylinder 5 is driven to rotate through the rotation of the installation cylinder 5 to carry out cell separation, a locking mechanism 6 is arranged between the installation cylinder 5 and the rotating cylinder 4, the locking mechanism 6 is used for installing the installation cylinder 5 inside the rotating cylinder 4 to prevent the installation cylinder 5 from flying out during rotation to cause accidents, and the locking mechanism 6 can be pasted or attracted by magnets and the like by using viscose glue; the filter cartridge 7 is arranged inside the mounting cylinder 5 in a sliding mode and is connected in a sliding mode, so that the mounting and dismounting of the filter cartridge 7 are facilitated, the use is convenient, the semipermeable membrane 8 is arranged inside the filter cartridge 7, the position of the semipermeable membrane 8 is arranged at the bottom of the filter cartridge 7, the filter cartridge 7 is arranged inside the mounting cylinder 5 in a sliding mode, a certain gap is formed between the bottom of the filter cartridge 7 and the bottom of the mounting cylinder 5, liquid molecules can be discharged conveniently during cell separation, cell separation operation can be completed better, separation can be achieved more thoroughly in the separation process, and the use is more convenient; a driving motor 2 is arranged in the working box 1, and the driving motor 2 is used for driving the rotating cylinder 4 to rotate; during the use, start driving motor 2, driving motor 2 rotates and drives a rotation section of thick bamboo 4 and rotate, and then drives cartridge filter 7 through an installation section of thick bamboo 5 and rotate at a high speed to through pellicle 8 with cell and liquid separation, easy operation is convenient, thereby improves this pellicle filtration separator's that preparation unicellular suspension was used practicality.

Mounting groove 3 has been seted up to 1 bottom of work box, and driving motor 2 is installed inside 3 mounting grooves, and 2 epaxial fixedly connected with dwang 201 of rotation of driving motor, dwang 201 rotate to be connected in 1 bottom of work box, and the one end and the 4 fixed connection of a rotating cylinder of driving motor 2 are kept away from to dwang 201.

Preferably, the bottom of the working box 1 is provided with a mounting groove 3, the driving motor 2 is mounted inside the mounting groove 3, and the driving motor 2 is mounted inside the working box 1, so that the volume of the working box 1 can be reduced, and the practicability of the working box 1 is improved; fixedly connected with dwang 201 in the axis of rotation of driving motor 2, wherein dwang 201 rotates to be connected in 1 bottom of work box, the one end and the 4 fixed connection of a rotating cylinder that driving motor 2 was kept away from to dwang 201, driving motor 2 starts during the use, the axis of rotation rotates and drives dwang 201 and rotate, and then drive a rotating cylinder 4 and rotate, wherein adopt dwang 201 to rotate and connect on work box 1, can make driving motor 2 only provide drive power for a rotating cylinder 4, need not to bear other effort, can protect driving motor 2's safe in utilization, improve driving motor 2's life.

An annular groove 701 is formed in the mounting cylinder 5, and the semipermeable membrane 8 is mounted on the mounting cylinder 5 through the annular groove 701.

Preferably, the semi-permeable membrane 8 is installed on the installation barrel 5 through the annular groove 701, a sealing device can be arranged between the annular groove 701 and the semi-permeable membrane 8, so that liquid which is not separated is prevented from overflowing from the joint of the annular groove 701 and the semi-permeable membrane 8 in the separation process, and the separation efficiency is improved.

Locking mechanism 6 includes spout 601, and spout 601 is seted up on rotating a section of thick bamboo 4, and it is provided with a joint pole 603 to slide in spout 601, is provided with spring 602 between joint pole 603 and the spout 601, has seted up joint groove 604 on the installation section of thick bamboo 5, and the elasticity of spring 602 orders about joint pole 603 joint and advances in joint groove 604, and the vertical spacing groove 605 of having seted up on the lateral wall of installation section of thick bamboo 5, and the vertical upwards slip setting of joint pole 603 is in spacing groove 605.

Preferably, the number of the locking mechanisms 6 can be 4, and the locking mechanisms are respectively distributed on four side surfaces of the installation cylinder 5, so that the installation cylinder 5 can be further stabilized inside the rotating cylinder 4, the sliding groove 601 is formed in the rotating cylinder 4, the clamping rod 603 is arranged inside the sliding groove 601 in a sliding manner, the clamping rod 603 extrudes the spring 602 when sliding inside the sliding groove 601, the installation cylinder 5 is provided with a clamping groove 604, the clamping rod 603 is in clamping fit with the clamping groove 604, one side of the clamping rod 603, which is close to the installation cylinder 5, is provided with a large radian, so that the clamping rod 603 and the clamping groove 604 are convenient to fit, and the clamping rod 603 is vertically and upwardly arranged in the limiting groove 605 in a sliding manner; when the installation barrel 5 needs to be installed inside the rotating barrel 4, the clamping rod 603 is firstly installed in the corresponding limiting groove 605 in a sliding manner, then the clamping rod 603 slides downwards, the limiting groove 605 extrudes the clamping rod 603 in the downward sliding process, the clamping rod 603 slides towards the sliding groove 601, the spring 602 is further extruded, and after the clamping rod 603 is driven to be clamped into the clamping groove 604 in the process of restoring the elasticity of the spring 602 after the clamping rod continues to slide downwards to the position of the clamping groove 604, so that the installation barrel 5 is fixed; the mounting tube 5 is removed in the opposite way, and will not be described in detail here.

A limiting block 401 is arranged in the rotating cylinder 4, and the mounting cylinder 5 is in butt fit with the limiting block 401.

Preferably, rotate 4 inside stopper 401 that are provided with of a section of thick bamboo, install a section of thick bamboo 5 and install inside rotating 4, install 5 bottoms of a section of thick bamboo and stopper 401 butt cooperation, stopper 401 plays the supporting role.

Be provided with spacing ring 11 in the installation section of thick bamboo 5, cartridge filter 7 and 11 butts of spacing ring, and liquid outlet 12 has been seted up to installation section of thick bamboo 5 bottom.

Preferably, a limiting ring 11 is arranged inside the mounting cylinder 5, and the limiting ring 11 is abutted against the filter cylinder 7 to support the filter cylinder 7; be provided with liquid outlet 12 in installation section of thick bamboo 5 bottom, be provided with the valve on liquid outlet 12, according to opening or closing of work option valve, inside the cartridge filter 7 is stayed to the in-process cell molecule of separation, other surplus liquid molecules then enter into installation section of thick bamboo 5 bottom through pellicle 8, are clearing up through liquid outlet 12.

The installation cylinder 5 is rotatably connected with a sealing cover 9, a buckle 901 is arranged on the sealing cover 9, a clamping groove 902 is formed in the installation cylinder 5, the buckle 901 is in clamping fit with the clamping groove 902, and an operation portion 10 is arranged on the sealing cover 9.

Preferably, in the course of working of carrying out the separation, sealed lid is closed on installation section of thick bamboo 5, and buckle 901 joint is inside draw-in groove 902 at this moment, and through the joint cooperation of buckle 901 with draw-in groove 902, can fix sealed lid 9 on installation section of thick bamboo 5 at the in-process of work, prevent cartridge filter 7 departure in the course of separating, cause the incident.

The working principle is as follows: the semipermeable membrane filtering and separating device for preparing the single cell suspension is used, firstly, a filter cylinder 7 placed with mixed liquid to be separated is arranged inside an installation cylinder 5, then the installation cylinder 5 is arranged inside a rotating cylinder 4, when the installation cylinder 5 needs to be arranged inside the rotating cylinder 4, a clamping rod 603 is firstly arranged in a corresponding limiting groove 605 in a sliding mode and then slides downwards, the limiting groove 605 extrudes the clamping rod 603 in the downward sliding process, the clamping rod 603 slides towards a sliding groove 601 and further extrudes a spring 602, after the clamping cylinder continues to slide downwards to the position of a clamping groove 604, the clamping rod 603 is driven to be clamped into the clamping groove 604 in the process that the spring 602 recovers elasticity, and then the installation cylinder 5 is fixed; after the installation is accomplished, start driving motor 2, driving motor 2 rotates and drives a rotation section of thick bamboo 4 and rotate, and then drives cartridge filter 7 through an installation section of thick bamboo 5 and rotate at a high speed to through pellicle 8 with cell and liquid separation, easy operation is convenient, thereby improves this pellicle filtration and separation device's that preparation unicellular suspension was used practicality.

While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the utility model. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the utility model.

Claims

1. The utility model provides a pellicle filter separator that preparation single cell suspension was used, includes work box (1), articulated on work box (1) have a case lid (101), its characterized in that: a rotating cylinder (4) is rotatably connected in the working box (1), an installation cylinder (5) is arranged in the rotating cylinder (4) in a sliding mode, a locking mechanism (6) is arranged between the installation cylinder (5) and the rotating cylinder (4), and the locking mechanism (6) plays a locking role in the installation cylinder (5);

a filter cylinder (7) is arranged in the mounting cylinder (5) in a sliding manner, and a semipermeable membrane (8) is arranged at the bottom of the filter cylinder (7);

a driving motor (2) is arranged in the working box (1), and the driving motor (2) is used for driving the rotating cylinder (4) to rotate.

2. The device of claim 1, wherein the semi-permeable membrane filtration separation device for preparing single cell suspension comprises: mounting groove (3) have been seted up to work box (1) bottom, install inside mounting groove (3) driving motor (2), driving motor (2) axis of rotation fixedly connected with one dwang (201), dwang (201) rotate to be connected in work box (1) bottom, the one end and a rotation section of thick bamboo (4) fixed connection of driving motor (2) are kept away from in dwang (201).

3. The device of claim 1, wherein the semi-permeable membrane filtration separation device for preparing single cell suspension comprises: an annular groove (701) is formed in the installation barrel (5), and the semipermeable membrane (8) is installed on the installation barrel (5) through the annular groove (701).

4. The device of claim 1, wherein the semi-permeable membrane filtration separation device for preparing single cell suspension comprises: the locking mechanism (6) comprises a sliding groove (601), the sliding groove (601) is formed in the rotating cylinder (4), a clamping rod (603) is arranged in the sliding groove (601) in a sliding mode, and a spring (602) is arranged between the clamping rod (603) and the sliding groove (601);

a clamping groove (604) is formed in the mounting barrel (5), and the elastic force of the spring (602) drives the clamping rod (603) to be clamped into the clamping groove (604);

a limiting groove (605) is vertically formed in the side wall of the mounting barrel (5), and the clamping rod (603) is vertically and upwards arranged in the limiting groove (605) in a sliding mode.

5. The device of claim 4, wherein the semi-permeable membrane is used for filtering and separating single cell suspension, and the device comprises: a limiting block (401) is arranged in the rotating cylinder (4), and the mounting cylinder (5) is in butt fit with the limiting block (401).

6. The device of claim 1, wherein the semi-permeable membrane filtration separation device for preparing single cell suspension comprises: a limiting ring (11) is arranged in the mounting cylinder (5), and the filter cylinder (7) is abutted against the limiting ring (11);

a liquid outlet (12) is arranged at the bottom of the mounting cylinder (5).

7. The device of claim 1, wherein the semi-permeable membrane filtration separation device for preparing single cell suspension comprises: the mounting cylinder (5) is rotatably connected with a sealing cover (9), a buckle (901) is arranged on the sealing cover (9), a clamping groove (902) is formed in the mounting cylinder (5), and the buckle (901) is in clamping fit with the clamping groove (902);

the sealing cover (9) is provided with an operating part (10).