CN215916523U

CN215916523U - Novel silica gel column chromatography device

Info

Publication number: CN215916523U
Application number: CN202122280953.6U
Authority: CN
Inventors: 周小波; 徐坤
Original assignee: Wuhan Huapu Biotechnology Co ltd
Current assignee: Wuhan Huapu Biotechnology Co ltd
Priority date: 2021-09-22
Filing date: 2021-09-22
Publication date: 2022-03-01
Anticipated expiration: 2031-09-22

Abstract

The utility model relates to the field of chromatographic separation, in particular to a novel silica gel column chromatography device. The device comprises a pretreatment column, a first pipeline, a second pipeline, a silica gel column, a third pipeline and a fourth pipeline; wherein, preliminary treatment top of the column and first pipe connection, preliminary treatment bottom of the column passes through three way connection spare with third pipeline and fourth pipeline and is connected, the fourth pipeline other end is connected with the receiving tank, first pipeline is connected to second pipeline one end, and the third pipeline is connected to the other end, third pipeline tip and silica gel column connection, the built-in upper and lower layer filter plate of preliminary treatment post fills the silica gel material between the filter plate for separate high polarity, high-viscosity solvent material, a large amount of impurity and difficult desorption material can be blockked in preliminary treatment post in the solvent, can not influence the silica gel column, thereby has prolonged the life of silica gel column.

Description

Novel silica gel column chromatography device

Technical Field

The utility model relates to the field of chromatographic separation, in particular to a novel silica gel column chromatography device.

Background

Silica gel is the most widely used chromatographic column filler at present due to its excellent mechanical strength and easy surface modification. Silica gel column has the characteristics of high column efficiency, good selectivity, high analysis speed and the like, so that the silica gel column is widely used for separating polar small molecules such as medicines.

The separation principle of silica gel chromatography is to separate substances according to different adsorption forces of the substances on silica gel, in general, substances with larger polarity are easy to be adsorbed by the silica gel, substances with weaker polarity are not easy to be adsorbed by the silica gel, and the whole chromatography process is the adsorption, desorption, re-adsorption and re-desorption processes.

Most of the existing silica gel columns are directly added with solvents or reaction liquid with different polarities into the silica gel columns for separation, so that a large amount of impurities and substances which are difficult to desorb in the solvents are remained in the silica gel columns, the solvents are difficult to clean, the service life of the silica gel columns is shortened, the purity requirements of separated samples cannot be met, the separation efficiency is low, and the defects exist.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model aims to overcome the defects of the prior art and provides a novel silica gel column chromatography device to solve the problems.

(II) technical scheme

The technical scheme of the utility model is as follows: a novel silica gel column chromatography device comprises: the device comprises a pretreatment column, a first pipeline, a second pipeline, a silica gel column, a third pipeline and a fourth pipeline; wherein, preliminary treatment top of the column portion and first pipe connection, preliminary treatment bottom of the column portion is connected through three way connection spare with third pipeline and fourth pipeline, the fourth pipeline other end is connected with the receiving tank, first pipeline is connected to second pipeline one end, and the third pipeline is connected to the other end, third pipeline tip and silica gel column connection, the built-in upper and lower layer filter plate of preliminary treatment post, packing silica gel material between the filter plate for separate high polarity, high viscosity solvent material.

The silica gel column includes the container that is formed by upper cover, column barrel and low head, be equipped with gas group mouth, feed inlet, dry by the fire the post discharge gate on the upper cover in proper order, feed inlet and third pipe connection, column barrel inboard top and bottom are equipped with upper filter plate and lower filter plate respectively, and the column barrel that is located the upper filter plate lower part is connected with into silica gel mouth for silica gel is reinforced, the low head is connected with the discharge gate, is located to be connected with between low head and the discharge gate and arranges the mouth, the discharge gate is connected with a plurality of branch mouths.

As a further improvement of the technical scheme of the utility model, the first pipeline, the second pipeline, the third pipeline and the fourth pipeline are provided with valves, so that the opening and closing of different pipelines can be conveniently controlled to select different working modes.

As a further improvement of the technical scheme of the utility model, the outer wall of the column cylinder body is provided with an outer coil pipe, when the silica gel is disassembled, steam is introduced into the column cylinder body, and the solvent in the silica gel column is dried.

As a further improvement of the technical scheme of the utility model, a pipeline connecting the discharge port and the branch pipe port is provided with a flowmeter for monitoring the flow of the sample after separation chromatography.

As a further improvement of the technical scheme of the utility model, a detection sampling port is arranged on a pipeline connecting the discharge port and the branch pipe port, so that the sampling detection of the sample after the separation chromatography is convenient.

(III) advantageous effects

The utility model has the advantages that:

1. a section of pretreatment column is arranged at the front end of the silica gel column, and a solvent can be controlled by a valve to enter the pretreatment column through a first pipeline for treatment; or the solvent can be controlled by a valve to directly enter the silica gel column through a second pipeline for separation; the bottom of the pretreatment column is connected with the silica gel column through a third pipeline, so that the pretreated solvent can be conveniently introduced into the silica gel column again for separation, or directly enters the receiving tank through a fourth pipeline, different working modes can be selected according to different conditions, the selectivity is more, the pertinence is stronger, and the silica gel column can be protected.

2. A large amount of impurities and difficult-to-desorb substances in the solvent can be blocked in the pretreatment column, so that the silica gel column is not influenced, and the service life of the silica gel column is prolonged;

3. the high-polarity and high-viscosity solvent material is directly eluted without entering the silica gel column, thereby accelerating the separation period of the silica gel column and improving the separation and production efficiency of the silica gel.

Drawings

FIG. 1 is a schematic view showing the connection between a pretreatment column and a silica gel column according to the present invention.

FIG. 2 is a schematic diagram of the silica gel column structure of the present invention.

Reference numerals: the device comprises a pretreatment column 1, a first pipeline 2, a second pipeline 3, a silica gel column 4, a gas group port 401, a feed inlet 402, a column drying discharge port 403, an upper seal head 404, an upper filter plate 405, an outer coil pipe 406, a column cylinder 407, a lower filter plate 408, a lower seal head 409, a discharge port 410, a detection sampling port 411, a branch pipe port 412, a flow meter 413, a clean discharge port 414, a silica gel inlet 415, a third pipeline 5 and a fourth pipeline 6.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Embodiment 1, please refer to fig. 1 and 2, a novel silica gel column chromatography apparatus includes a pretreatment column 1, a first pipeline 2, a second pipeline 3, a silica gel column 4, a third pipeline 5 and a fourth pipeline 6; wherein, the pretreatment column 1 top is connected with first pipeline 2, pretreatment column 1 bottom is connected through tee junction spare with third pipeline 5 and fourth pipeline 6, first pipeline 2 is connected to 3 one end of second pipeline, and third pipeline 5 is connected to the other end, third pipeline 5 tip is connected with silica gel column 4, pretreatment column 1 embeds the filter plate, silica gel column 4 includes the container that is formed by upper cover 404, column body 407 and low head 409, be equipped with gas group mouth 401 on the upper cover 404 in proper order, feed inlet 402, dry by fire post discharge gate 403, feed inlet 402 is connected with third pipeline 5, the inboard top of column body 407 is equipped with upper filter plate 405 and lower filter plate 408 respectively with the bottom, the column body 407 that is located the lower part of upper filter plate 405 is connected with into silica gel mouth 415, column body outer wall 407 is equipped with outer coil pipe 406, when can dismantle silica gel, lead to steam inside, drying the solvent in the silica gel column, wherein the lower end enclosure 409 is connected with a discharge port 410, a drain port 414 is connected between the lower end enclosure 409 and the discharge port 410, and the discharge port 410 is connected with a plurality of branch pipe ports 412.

Specifically, first pipeline 2, second pipeline 3, third pipeline 5 and fourth pipeline 6 all are equipped with the valve, are convenient for control different pipelines and open and shut in order to select different working methods.

Specifically, the pipeline connecting the discharge port 410 and the branch port 412 is provided with a flow meter 413 for monitoring the flow of the sample after separation chromatography.

Specifically, the pipeline connecting the discharge port 410 and the branch port 412 is provided with a detection sampling port 411, which is convenient for detecting the sample after separation chromatography.

The working principle is as follows:

a section of pretreatment column 1 is arranged at the front end of a silica gel column 4, and solvents with different polarities can be controlled by a valve to enter the pretreatment column 1 through a first pipeline 2 for eluting the solvents;

the bottom of the pretreatment column 1 is connected with the silica gel column 4 through a third pipeline 5, the solvent which is pretreated and then leaves the pretreatment column 1 can be introduced into the silica gel column 4 again through the third pipeline 5 for separation and chromatography, and the filter plate on the inner side of the silica gel column 4 can prevent the silica gel from leaking outside;

after other components in the pretreatment column 1 are completely eluted into the silica gel column 4, the solvent with the highest polarity and the highest viscosity still remains in the pretreatment column 1;

after the solvent in the silica gel column 4 is separated, the valve of the second pipeline 3 is closed, the valves on the first pipeline 2 and the fourth pipeline 6 are opened, the high-polarity solvent enters the pretreatment column 1 through the first pipeline 2, the eluent does not enter the silica gel column 4, but directly enters the receiving tank through the fourth pipeline 6, different working modes can be selected according to different conditions, the selectivity is more, the pertinence is stronger, and the silica gel column is protected.

The flow meter 413 and the detection sampling port 411 are respectively arranged on the connecting pipeline of the discharge port 410 and the branch port 412, so that the flow monitoring and the sample sampling detection can be carried out on the sample after the separation chromatography.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A novel silica gel column chromatography device is characterized by comprising: the device comprises a pretreatment column (1), a first pipeline (2), a second pipeline (3), a silica gel column (4), a third pipeline (5) and a fourth pipeline (6); wherein, preliminary treatment post (1) top is connected with first pipeline (2), preliminary treatment post (1) bottom is connected through tee junction spare with third pipeline (5) and fourth pipeline (6), first pipeline (2) are connected to second pipeline (3) one end, and third pipeline (5) are connected to the other end, third pipeline (5) tip is connected with silica gel column (4), the built-in filter plate of preliminary treatment post (1) and silica gel.

2. The novel silica gel column chromatography device according to claim 1, wherein: silica gel column (4) include the container that is formed by upper cover (404), column barrel (407) and low head (409), be equipped with gas group mouth (401), feed inlet (402) on upper cover (404) in proper order, dry by the fire post discharge gate (403), feed inlet (402) are connected with third pipeline (5), column barrel (407) inboard top is equipped with filter plate (405) and lower filter plate (408) respectively with the bottom, and column barrel (407) that are located upper filter plate (405) lower part are connected with into silica gel mouth (415), low head (409) are connected with discharge gate (410), are located and are connected with between low head (409) and discharge gate (410) and arrange clean mouth (414), discharge gate (410) are connected with a plurality of nozzles (412).

3. The novel silica gel column chromatography device according to claim 1, wherein: the first pipeline (2), the second pipeline (3), the third pipeline (5) and the fourth pipeline (6) are all provided with valves.

4. The novel silica gel column chromatography device according to claim 2, characterized in that: an outer coil (406) is arranged on the outer wall of the column body (407).

5. The novel silica gel column chromatography device according to claim 2, characterized in that: the pipeline connecting the discharge port (410) and the branch pipe port (412) is provided with a flowmeter (413).

6. The novel silica gel column chromatography device according to claim 2, characterized in that: the pipeline connecting the discharge port (410) and the branch pipe port (412) is provided with a detection sampling port (411).