CN215894160U - Plankton counting frame easy for sealing - Google Patents
Plankton counting frame easy for sealing Download PDFInfo
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- CN215894160U CN215894160U CN202122145376.XU CN202122145376U CN215894160U CN 215894160 U CN215894160 U CN 215894160U CN 202122145376 U CN202122145376 U CN 202122145376U CN 215894160 U CN215894160 U CN 215894160U
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- cover glass
- plate body
- plankton
- glycerol
- sample cell
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Abstract
The utility model relates to a plankton counting frame easy for sealing, which comprises a plate body; the surface of the plate body is provided with a sunken sample cell, a counting grid is fixedly arranged in the sample cell, the surface of the plate body around the sample cell forms a glycerol smearing region, a cover glass positioning plate is fixedly arranged at the periphery of the glycerol smearing region, and the cover glass positioning plate is fixed on the plate body and is higher than the surface of the plate body; the cover glass can cover the sample pool, and one side of the cover glass is in contact with the cover glass positioning plate. According to the counting frame, the cover glass baffle is arranged, so that the position of the cover glass can be accurately positioned, bubbles are prevented from being generated, and the cover glass is easy to seal; the glycerol smearing area of the plate body is limited, so that a user can conveniently smear and seal glycerol; four sides of the plate body of the counting frame adopt a frosted design, so that deviation is not easily generated in the taking and moving process, the stability and effectiveness of plankton counting are improved, and the experimental error is reduced.
Description
Technical Field
The utility model belongs to the technical field of aquatic ecology research, and particularly relates to a plankton counting frame easy to seal.
Background
Plankton (plankton) is commonly found in various water bodies such as reservoirs, lakes, rivers, ponds and the like, and generally refers to the plankton living in water environment and lacking effective moving capability, and comprises phytoplankton and zooplankton. Phytoplankton, as a primary consumer of the water environment, is an important component of aquatic ecosystems. The individual is small, the life cycle of the individual is usually short, the passage speed is high, the individual is sensitive to the change of the water environment, and the individual is usually used as one of important indexes of the water quality condition. Zooplankton, as a unicellular organism, is diverse in form, wide in variety, and extremely widespread, playing a very important role in material circulation and energy exchange, and therefore, many phytoplankton and zooplankton are used as indicator organisms of water pollution.
In ecological monitoring, the qualitative and quantitative analysis of plankton is generally manual detection, and plankton in a water sample needs to be identified and counted under an optical microscope so as to judge the water quality condition of a sampled water body.
However, the existing biotechnology has many problems: 1. generally, the time for manual identification and counting is long, and a water sample is easy to volatilize, so that the edge of the water sample shrinks towards the center in a counting plate, the position of a part of plankton is moved, the situations of repeated counting, less counting and missing recording are caused, and the accuracy of a final result is directly influenced; 2. the volatilization of water in a water sample to be detected can be condensed on the objective lens, so that the lens is fuzzy, the identification difficulty of experimenters is increased, and meanwhile, the aging of the objective lens can be accelerated; 3. the area of a sample pool of a common plankton counting frame is larger than that of a cover glass, and after the cover glass is covered, a water sample overflows from the edge of the cover glass; 4. the common plankton counting frame is not provided with an auxiliary placing device when a cover glass is placed, so that the common plankton counting frame is easy to incline and incline when the cover glass is placed, partial planktons are not in a counting area, and the error of an experimental result is large; 5. the common plankton counting frame is not provided with an auxiliary placing device, so that bubbles with different sizes are easily generated when the cover glass is placed; 6. when detecting, because of lack of fixed mode, can make plankton count board produce and rock, influence the stability of operation.
In order to solve the above problems, there is a need to develop a plankton counting frame with simple structure, delicate design, accurate positioning of the cover glass, and easy mounting.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art and provide the plankton counting frame which has the advantages of simple structure, exquisite design, accurate positioning of a cover glass and easy mounting.
The technical problem to be solved by the utility model is realized by adopting the following technical scheme:
the plankton counting frame easy for sealing the sheet comprises a plate body; the surface of the plate body is provided with a sunken sample cell, a counting grid is fixedly arranged in the sample cell, the surface of the plate body around the sample cell forms a glycerol smearing region, a cover glass positioning plate is fixedly arranged at the periphery of the glycerol smearing region, and the cover glass positioning plate is fixed on the plate body and is higher than the surface of the plate body;
the cover glass can cover the sample pool, and one side of the cover glass is in contact with the cover glass positioning plate.
Furthermore, a frosted surface is formed on the side wall of the plate body.
Further, the cover glass size is not smaller than the size of the glycerol smearing region.
Furthermore, the depth of the pits in each sample cell is uniformly distributed.
Further, the shape of the sample cell is square.
The utility model has the advantages and positive effects that:
according to the plankton counting frame, the cover glass baffle is arranged, so that the position of the cover glass can be accurately positioned, bubbles are prevented from being generated, and the cover glass is easy to seal; the glycerol smearing area of the plate body is limited, so that a user can conveniently smear and seal glycerol; four sides of the plate body of the counting frame adopt a frosted design, so that deviation is not easily generated in the taking and moving process, the stability and effectiveness of plankton counting are improved, and the experimental error is reduced.
Drawings
The technical solutions of the present invention will be described in further detail below with reference to the accompanying drawings and examples, but it should be understood that these drawings are designed for illustrative purposes only and thus do not limit the scope of the present invention. Furthermore, unless otherwise indicated, the drawings are intended to be illustrative of the structural configurations described herein and are not necessarily drawn to scale.
FIG. 1 is a schematic structural diagram of a plankton counting frame for easy mounting according to an embodiment of the present invention;
FIG. 2 is a top view of the structure of the plankton counting frame for easy mounting according to the embodiment of the present invention;
FIG. 3 is a cross-sectional view of the plankton counting frame for easy mounting according to the embodiment of the present invention;
Detailed Description
First, it should be noted that the specific structures, features, advantages, etc. of the present invention will be specifically described below by way of example, but all the descriptions are for illustrative purposes only and should not be construed as limiting the present invention in any way. Furthermore, any individual technical features described or implicit in the embodiments mentioned herein may still be continued in any combination or subtraction between these technical features (or their equivalents) to obtain still further embodiments of the utility model that may not be mentioned directly herein.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.
As shown in fig. 1 to 3, the present invention provides a plankton counting frame for easy mounting, comprising a plate body 1; a concave sample cell 2 is arranged on the surface of the plate body, a counting grid 10 is fixedly arranged in the sample cell, a glycerol smearing region 4 is formed on the surface of the plate body around the sample cell 2, a cover glass positioning plate 3 is fixedly arranged on the periphery of the glycerol smearing region, and the cover glass positioning plate is fixed on the plate body and is higher than the surface of the plate body;
the device also comprises a cover glass 9, wherein the cover glass can cover the sample pool, and one side of the cover glass is contacted with the cover glass positioning plate.
As shown in fig. 1, the front side 5, the rear side 6, the left side 7 and the right side 8 of the plate body are all subjected to sanding treatment, so that the stability of taking and moving on a microscope objective table by an experimenter can be effectively improved;
the cover glass size is not smaller than the size formed by the glycerol smearing region, and the cover glass 9 should be not smaller than the outer edge size of the glycerol smearing region 4.
The depth of the pits at each position in the sample cell is uniformly distributed, the sample cell is square, the square sample cell 2 is arranged in the plate body 1, the bottom of the sample cell 2 is provided with a counting grid 10, and the volume of the sample cell 2 can be 0.1ml, 1ml, 5ml or higher according to the use requirement;
according to the plankton counting frame, the cover glass baffle is arranged, so that the position of the cover glass can be accurately positioned, bubbles are prevented from being generated, and the cover glass is easy to seal; the glycerol smearing area of the plate body is limited, so that a user can conveniently smear and seal glycerol; the four side edges of the plate body of the counting frame are designed by adopting frosting, so that deviation is not easy to generate in the taking and moving processes, the stability and effectiveness of plankton counting are improved, and experimental errors are reduced;
according to the plankton counting frame, the matched reagent is applied, so that the difficulty of laboratory personnel in mounting the sealing piece is reduced when the plankton counting frame is used for mounting the sealing piece, the possibility of generating bubbles in a sealing piece sample is reduced, moisture in a sample to be detected of planktons is kept for a long time, the stability of an experimental structure is ensured, the definition of an objective lens is ensured, the identification difficulty of the laboratory personnel is reduced, the certainty of the position of the planktons in the sample is ensured, the detection effectiveness is ensured, and the movement and the stable fixation are convenient.
For example, in this embodiment, first, the plankton counting frame is flatly placed on a horizontal desktop, glycerol is uniformly smeared in the glycerol smearing region by a glycerol-soaked cotton swab or other similar tools, an experimental sample is accurately pipetted by a pipette, the experimental sample is pipetted into a sample cell, the sample cell is uniformly filled with a water sample, if the water sample forms a water film due to surface tension, the water sample is destroyed by a pipette head, the surface of the water sample is flat, a cover glass is aligned at 45 ° with a positioning plate and abuts against an included angle between the positioning plate and the glycerol smearing region, the cover glass is covered on the sample cell downwards along the included angle between the positioning plate and the glycerol smearing region, and then the sealing treatment of the plankton counting frame is completed.
The present invention has been described in detail with reference to the above examples, but the description is only for the preferred examples of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.
Claims (5)
1. Plankton count frame of easy mounting, its characterized in that: comprises a plate body; the surface of the plate body is provided with a sunken sample cell, a counting grid is fixedly arranged in the sample cell, the surface of the plate body around the sample cell forms a glycerol smearing region, a cover glass positioning plate is fixedly arranged at the periphery of the glycerol smearing region, and the cover glass positioning plate is fixed on the plate body and is higher than the surface of the plate body;
the cover glass can cover the sample pool, and one side of the cover glass is in contact with the cover glass positioning plate.
2. The plankton counting frame easy to cover sheet of claim 1, wherein: and a frosted surface is formed on the side wall of the plate body.
3. The plankton counting frame easy to cover sheet of claim 1, wherein: the cover glass size is not smaller than the size formed by the glycerol smearing region.
4. The plankton counting frame easy to cover sheet of claim 1, wherein: the depth of the pits in each sample cell is uniformly distributed.
5. The plankton counting frame easy to cover sheet of claim 1, wherein: the sample cell is square in shape.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202122145376.XU CN215894160U (en) | 2021-09-07 | 2021-09-07 | Plankton counting frame easy for sealing |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202122145376.XU CN215894160U (en) | 2021-09-07 | 2021-09-07 | Plankton counting frame easy for sealing |
Publications (1)
Publication Number | Publication Date |
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CN215894160U true CN215894160U (en) | 2022-02-22 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN202122145376.XU Active CN215894160U (en) | 2021-09-07 | 2021-09-07 | Plankton counting frame easy for sealing |
Country Status (1)
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CN (1) | CN215894160U (en) |
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2021
- 2021-09-07 CN CN202122145376.XU patent/CN215894160U/en active Active
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