CN215799635U

CN215799635U - Micro-fluidic system for simulating atomized medicament to treat lung cancer cells

Info

Publication number: CN215799635U
Application number: CN202120771189.XU
Authority: CN
Inventors: 尹彬沣; 胡立鹏; 胡立雨
Original assignee: Yangzhou University
Current assignee: Yangzhou University
Priority date: 2021-04-15
Filing date: 2021-04-15
Publication date: 2022-02-11
Anticipated expiration: 2031-04-15

Abstract

The utility model discloses a micro-fluidic system for simulating atomized medicament to treat lung cancer cells in the technical field of micro-fluidic, which comprises an upper chip body, a cell culture chip body with a containing cavity, a micro-channel chip body and a lower chip body which are sequentially connected together from top to bottom, wherein the upper chip body is provided with a sample adding hole, the cell culture chip body is provided with a plurality of cell culture holes, the cell culture chip body and the micro-channel chip body are respectively provided with a through hole and a sample injection groove which correspond to the sample adding hole, the micro-channel chip body is provided with a plurality of through holes which correspond to the cell culture holes one by one, the micro-channel chip body in the containing cavity is provided with a plurality of micro-channels, the micro-channel chip body is provided with a metabolic liquid collecting port, two ends of the containing cavity are respectively communicated with the sample injection groove and the metabolic liquid collecting port, the lower chip body is provided with a plurality of cell culture chambers which correspond to the through holes one by one, a metabolic liquid collecting tank is arranged on the lower chip body; the utility model can be used for conveniently detecting the treatment effect of the cancer cells.

Description

Micro-fluidic system for simulating atomized medicament to treat lung cancer cells

Technical Field

The utility model belongs to the technical field of microfluidics, and particularly relates to a microfluidic system for simulating atomized medicament to treat lung cancer cells.

Background

Primary bronchogenic carcinoma, lung cancer for short, the english name lung cancer, refers to a malignant tumor that originates in the trachea, bronchi and lungs. Lung cancer is a bronchogenic cancer, including several major types of squamous cell carcinoma, adenocarcinoma, small cell carcinoma and large cell carcinoma. The incidence and mortality of lung cancer is rapidly rising and is a worldwide trend. In the condition that the early symptoms of lung cancer are not obvious, most lung cancer patients are diagnosed at an advanced stage, and some patients have poor constitution and cannot bear traditional surgery and chemotherapy. In this case, aerosolized medication may be their first choice. The method can rapidly inhibit and kill cancer cells, and has no obvious side effect.

The organ chip is a carrier of the micro-fluidic chip, combines with the cell technology to carry out the advanced technology of scientific research, can be used for a plurality of functions such as detection, experiments, drug research and the like, and is suitable for the fields of biology and medicine. The micro-fluidic chip is also called a lab-on-a-chip, is an emerging scientific technology which is operated by a specific fluid, can provide a physiological environment which is closer to the human body, integrates basic operation units of sample preparation, reaction, separation, detection, cell culture, sorting, lysis and the like which are related to the chemical and biological fields on a chip with a size of a few square centimeters or less, simultaneously measures the related quantities of physics, chemistry and biology, is a mainstream platform for accurately operating mammalian cells and the microenvironment thereof in combination with the cell technology, and is evaluated as one of ten new technologies in the 2016 world by the economic forum in the world.

In the prior art, a chinese patent invention, entitled "organ chip unit including pipeline network", publication No. CN 108004144 a, publication No. 2018.05.18, is disclosed, the chip unit includes at least one layer of chip including input pipeline network for input, diversion, and diffusion of substances, at least one layer of chip including output pipeline network for diffusion, confluence, and output of substances; at least one layer of cavity chip, wherein the cavity chip is used for containing parenchymal organ tissues; at least two layers of microporous membranes, wherein the parenchymal organ tissues and the input and output pipeline networks are separated by the microporous membranes; at least two layers of barrier cells, wherein the barrier cells are positioned in the input pipeline network and the output pipeline network and are adhered to the microporous membrane; the two layers of sealing plates are used for realizing packaging, and the chip can simulate the process that substances enter tissues after being shunted through various pipeline networks such as blood vessels and lymphatic vessels and gradually converge after being metabolized in the tissues, but cannot realize the detection of the treatment effect of the drugs on cancer cells.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defects in the prior art, provides the micro-fluidic system for simulating the atomized medicament to treat the lung cancer cells, solves the technical problem that the medicament detection cannot be realized in the prior art, has a reliable structure, can facilitate the detection of the medicament on the treatment effect of the cancer cells, and is convenient to operate.

The purpose of the utility model is realized as follows: the utility model provides a micro-fluidic system of simulation atomized medicament to lung cancer cell therapy, includes by last upper chip body, cell culture chip body, microchannel chip body and the lower floor chip body that links together in proper order from top to bottom, it has the application of sample hole to open on the upper chip body, a plurality of cell culture holes of having arranged on the cell culture chip body, it has the feed liquor hole that corresponds with the application of sample hole to open on the cell culture chip body, it has the sampling groove that corresponds with application of sample hole position to open on the microchannel chip body, a plurality of through-holes with cell culture hole one-to-one have arranged on the microchannel chip body, the microchannel chip body has the chamber of holding, hold chamber one end and sampling groove intercommunication in the left and right directions, a plurality of microchannels of having arranged on the microchannel chip body in the chamber, the through-hole is between each microchannel, be equipped with the polytetrafluoroethylene membrane between cell culture chip body and the microchannel chip body, it has the metabolism liquid to collect the mouth to open on the microchannel chip body, and a plurality of liquid holes of having arranged on the lateral wall that feed liquor groove one end was kept away from to the microchannel chip body, hold the chamber other end and go out liquid hole intercommunication about the ascending side, a plurality of cell culture rooms with the through-hole one-to-one of having arranged on the lower floor's chip body, lower floor's chip body one end up is opened has the metabolism liquid collecting vat that corresponds with the metabolism liquid collection mouth, and it has inspection hole one to open on the upper chip body, and it has inspection hole two that corresponds with inspection hole one to open on the cell culture body, the metabolism liquid collection mouth covers the area of inspection hole two.

In the utility model, an upper chip body, a cell culture chip body, a polytetrafluoroethylene membrane, a microchannel chip body and a lower chip body are sequentially connected together, and the peripheries of the bodies are aligned; firstly planting lower layer cells, specifically, injecting cell suspension liquid through a sample injection hole, enabling the cell suspension liquid to sequentially enter a containing cavity through a sample injection groove, enabling the cell suspension liquid entering the containing cavity to flow between micro-channels, enabling the cell suspension liquid to downwards flow into a cell culture chamber along a through hole at the lower part when the cell suspension liquid passes through the through hole, enabling redundant cell culture liquid to sequentially flow into a metabolic liquid collecting tank through a liquid outlet hole and a metabolic liquid collecting port, injecting cell culture liquid through the sample injection hole after the lower layer cells are planted, providing nutrient substances for the upper layer cells and the lower layer cells, planting upper layer cells, injecting cell suspension liquid into the cell culture hole, enabling the cell suspension liquid to sequentially flow into the cell culture chamber through a part of the cell culture hole, the polytetrafluoroethylene membrane and the through hole, and enabling the other part to sequentially flow into the metabolic liquid collecting tank through the micro-channels, the liquid outlet hole and the metabolic liquid collecting port, after the upper layer of cells are planted, sterile air is introduced through the cell culture holes to prepare for next ventilation of sol medicines, after a period of time, redundant suspension liquid is discharged, atomized medicines begin to be introduced through the cell culture holes, a part of atomized medicines also flow into the metabolic liquid collecting tank, metabolic liquid in the metabolic liquid collecting tank is extracted through the detection holes I and the detection holes II, and the treatment effect of the medicines on the treatment of corresponding diseases can be achieved by detecting the extracted metabolic liquid; can be applied to the work of drug detection.

In order to further facilitate the planting of the upper layer cells, a drug inlet groove is formed in the upper layer chip body, a drug cavity communicated with the drug inlet groove is formed in the downward end of the upper layer chip body, and the drug cavity covers the area where all the cell culture holes are located.

As a further improvement of the utility model, the downward end of the cell culture chip body is also provided with a metabolic liquid collecting cavity corresponding to the metabolic collecting port.

Drawings

Fig. 1 is a perspective view of the present invention.

Fig. 2 is an exploded view of the present invention.

Fig. 3 is a three-dimensional structure diagram of the upper chip body according to the utility model.

FIG. 4 is a perspective view of the cell culture chip body according to the present invention.

FIG. 5 is a perspective view of the internal structure of the microchannel chip body of the present invention.

Fig. 6 is a perspective view of the lower chip body according to the present invention.

The cell culture device comprises a cell culture hole 1, a cell culture chip body 2, a detection hole II 3, a polytetrafluoroethylene membrane 4, a metabolic liquid collection port 5, a microchannel chip body 6, a metabolic liquid collection tank 7, a cell culture chamber 8, a lower chip body 9, a sample injection groove 10, a through hole 11, a liquid outlet hole 12, a detection hole I13, a liquid inlet hole 14, a sample injection hole 15, a drug injection groove 16, an upper chip body 17, a drug cavity 18, a metabolic liquid collection cavity 19 and a microchannel 20.

Detailed Description

The utility model is further described below with reference to the accompanying drawings.

As shown in figures 1-6, a micro-fluidic system for simulating atomized medicament to treat lung cancer cells comprises an upper chip body 17, a cell culture chip body 2, a micro-channel chip body 6 and a lower chip body 9 which are sequentially connected together from top to bottom, wherein the upper chip body 17 is provided with a sample adding hole 15, the cell culture chip body 2 is provided with a plurality of cell culture holes 1, the cell culture chip body 2 is provided with a liquid inlet hole 14 corresponding to the sample adding hole 15, the micro-channel chip body 6 is provided with a sample feeding groove 10 corresponding to the sample adding hole 15, the micro-channel chip body 6 is provided with a plurality of through holes 11 corresponding to the cell culture holes 1 one by one, the micro-channel chip body 6 is provided with a containing cavity, one end of the containing cavity in the left-right direction is communicated with the sample feeding groove 10, the micro-channel chip body 6 in the containing cavity is provided with a plurality of micro-channels 20, and the through holes 11 are arranged among the micro-channels 20, a polytetrafluoroethylene membrane 4 is arranged between the cell culture chip body 2 and the micro-channel chip body 6, a metabolic liquid collecting port 5 is arranged on the micro-channel chip body 6, a plurality of liquid outlet holes 12 are arranged on the side wall of one end of the micro-channel chip body 6, which is far away from the liquid inlet groove, the other end of the containing cavity in the left-right direction is communicated with the liquid outlet holes 12, a plurality of cell culture chambers 8 which are in one-to-one correspondence with the through holes 11 are arranged on the lower chip body 9, a metabolic liquid collecting tank 7 which is corresponding to the metabolic liquid collecting port 5 is arranged at the upward end of the lower chip body 9, a metabolic liquid collecting cavity 19 which is corresponding to the metabolic liquid collecting port is also arranged at the downward end of the cell culture chip body 2, the metabolic liquid collecting cavity 19, the metabolic liquid collecting port 5 and the metabolic liquid collecting tank 7 form a metabolic liquid collecting pool, a detection hole I13 is arranged on the upper chip body 17, a detection hole II 3 which is corresponding to the detection hole I13 is arranged on the cell culture chip body, the metabolic fluid collecting port 5 covers the area of the second detection hole 3.

In order to further facilitate the planting of the upper layer cells, the upper layer chip body 17 is provided with a drug inlet groove 16, one downward end of the upper layer chip body 17 is provided with a drug cavity 18 communicated with the drug inlet groove 16, and the drug cavity 18 covers the area of all the cell culture holes 1.

In the utility model, an upper chip body 17, a cell culture chip body 2, a polytetrafluoroethylene membrane 4, a microchannel chip body 6 and a lower chip body 9 are sequentially connected together, and the peripheries of the bodies are aligned; firstly planting lower layer cells which can be other human body cells except lung cells, such as heart cells and liver cells, by planting a multi-cell culture chip, wherein the lower layer cells are injected by a sample adding hole 15, the cell suspension liquid sequentially enters a containing cavity through a liquid inlet hole 14 and a sample adding groove 10, the cell suspension liquid entering the containing cavity flows between microchannels 20, flows downwards to a cell culture chamber 8 along a through hole 11 at the lower part when passing through a through hole 11, redundant cell culture liquid flows into a metabolic liquid collecting groove 7 through a liquid outlet hole 12, after the lower layer cells are planted, the cell culture liquid is injected by the sample adding hole 15 to provide nutrient substances for the upper layer cells and the lower layer cells, and after the injection is finished, the upper layer cells are planted, in the embodiment, the upper layer cells are lung cancer cells and macrophages, and the planting method is specifically that the cell suspension liquid is continuously injected into a drug adding groove 16, the cell suspension enters a drug cavity 18 along a drug inlet groove 16 and flows downwards through cell culture holes 1, the speed of liquid drug entering the drug inlet groove 16 is higher than the speed of liquid drug exiting through the cell culture holes 1, so that the area of the drug cavity 18 is covered with the cell suspension, the cell suspension passes through each cell culture hole 1, the cell suspension flowing down from the cell culture holes 1 sequentially flows into a containing cavity through a polytetrafluoroethylene membrane 4 and a through hole 11 at the upper part, one part of the cell suspension flows downwards into a cell culture chamber 8, the other part of the cell suspension sequentially flows into a metabolic liquid collecting tank 7 through a micro-channel 20 and a liquid outlet hole 12, after the upper layer of cell planting is finished, sterile air is introduced through the drug inlet groove 16 to prepare for next ventilation of sol drugs, after a period of time, the redundant suspension is discharged, the atomized drugs begin to be introduced through the drug inlet groove 16, and the cancer cells are influenced by the atomized drugs and factors secreted by the bottom layer of cells, the macrophage is further influenced, the amount of TNF-alpha secreted by the macrophage is changed, the TNF-alpha passes through the polytetrafluoroethylene membrane 4 to the microchannel 20 and then flows into the metabolic fluid pool, and the treatment effect of the drug on treating the cancer can be judged by detecting the concentration change of the TNF-alpha; during implementation, a part of atomized medicament also flows into the metabolic fluid collecting tank 7, metabolic fluid in the metabolic fluid collecting tank 7 can be extracted through the first detection hole 13 and the second detection hole 3, the concentration of TNF-alpha in the extracted metabolic fluid is detected, and the treatment effect of the medicament on treating cancer cells is analyzed by comparing the change of the antigen concentration in each time period when the medicament is not added and when the medicament is added; the drug can act on the cells at the lower layer, and the cell culture chamber 8 cultures the common cells, so that the cells except the lung are simulated, and whether the anti-cancer drug is harmful to the healthy cells can be further analyzed; the device has a reliable structure, simulates the internal tissue environment of a human body, can extract the metabolic fluid in the metabolic collection pool to realize the detection of the anti-cancer effect of the drug, and is convenient to detect; can be applied to the detection of the markers of the lung cancer cell metabolic fluid.

The present invention is not limited to the above-mentioned embodiments, and based on the technical solutions disclosed in the present invention, those skilled in the art can make some substitutions and modifications to some technical features without creative efforts according to the disclosed technical contents, and these substitutions and modifications are all within the protection scope of the present invention.

Claims

1. The utility model provides a micro-fluidic system of simulation atomized medicament to lung cancer cell therapy, its characterized in that includes by upper chip body, cell culture chip body, microchannel chip body and lower floor chip body that from the top down links gradually together, it has the application of sample hole to open on the upper chip body, a plurality of cell culture holes of having arranged on the cell culture chip body, it has the feed liquor hole that corresponds with the application of sample hole to open on the cell culture chip body, it has the sampling groove that corresponds with application of sample hole position to open on the microchannel chip body, a plurality of through-holes with cell culture hole one-to-one have arranged on the microchannel chip body, the microchannel chip body has the chamber of holding, the chamber of holding is one end and sampling groove intercommunication on the left and right sides, a plurality of microchannels of having arranged on the microchannel chip body of holding the intracavity, the through-hole is between each microchannel, cell culture chip body and microchannel chip body are equipped with the polytetrafluoroethylene membrane between, and it has the metabolic liquid to collect the mouth to open on the microchannel chip body, and microchannel chip body keeps away from a plurality of liquid holes of having arranged on the lateral wall of feed liquor groove one end, hold the chamber other end and go out liquid hole intercommunication in the left right direction, a plurality of cell culture rooms with the through-hole one-to-one of having arranged on the chip body of lower floor, the ascending one end of lower floor chip body is opened has the metabolic liquid collecting vat that corresponds with the metabolic liquid collection mouth, and it has inspection hole one to open on the chip body of upper strata, and it has inspection hole two that corresponds with inspection hole one to open on the cell culture body, metabolic liquid collection mouth covers inspection hole two places region.

2. The microfluidic system for simulating atomized medicament to treat lung cancer cells according to claim 1, wherein the upper chip body is provided with a medicament inlet groove, one downward end of the upper chip body is provided with a medicament chamber communicated with the medicament inlet groove, and the medicament chamber covers the area of all the cell culture holes.

3. The microfluidic system for simulating the atomized medicament to treat the lung cancer cells according to claim 1 or 2, wherein a metabolic fluid collecting chamber corresponding to the metabolic collecting port is further arranged at the downward end of the cell culture chip body.