CN215599081U - Metabolism micromolecule sample detecting system - Google Patents

Abstract

The utility model discloses a metabolic small molecule sample detection system, which relates to the technical field of sample detection and comprises a control center, a magnetic suction module, a gun arranging module, a reagent carrier and a shell module, wherein the control center is connected with the magnetic suction module, the gun arranging module, the reagent carrier and the shell module; the rifle arranging module comprises a suction and discharge structure and a moving module, and a liquid suction structure and a liquid discharge structure are arranged in the suction and discharge structure; the mobile module is connected with a motor. Has the following beneficial effects: (1) the structure is clear, and the operation is convenient. (2) The sample pretreatment operation is efficient and quick. (3) The automatic detection device is adapted, and the compatibility is good.

Description

Metabolism micromolecule sample detecting system

Technical Field

The utility model relates to the technical field of sample detection, in particular to a metabolic small molecule sample detection system.

Background

At present, a plurality of small molecule sample detection methods exist in the market, wherein the immune method can realize automatic operation, has short detection time, is a common detection method in clinical laboratories at present, but the use of the immune method is influenced by the existence of the defects of easy interference of cross reaction, narrow linear range and the like due to the characteristics of antigen-antibody reaction. The detection principle of the mass spectrometry can ensure accurate quantification of analytes, and the mass spectrometry is an internationally recognized analysis method capable of accurately determining small molecular compounds and steroid hormones, but has many operation steps, cannot be automated and the like, and seriously hinders clinical application. The research and development of the automatic detection device help the detection method to detect the small molecule sample more quickly, efficiently and accurately. There is a need for a control system for an adaptive automated inspection device.

SUMMERY OF THE UTILITY MODEL

1. Technical problem to be solved by the utility model

Aiming at the technical problem that the automatic detection device needs an adaptive control system urgently, the utility model provides a metabolic small molecule sample detection system, wherein a control center of the metabolic small molecule sample detection system is connected with a magnetic suction module, a gun arrangement module, a reagent carrier and a shell module, and software is arranged in the control center to control the modules to cooperate with each other to finish the automatic detection of the small molecule sample.

2. Technical scheme

In order to solve the problems, the technical scheme provided by the utility model is as follows:

a metabolic small molecule sample detection system comprises a control center, a magnetic suction module, a gun arranging module, a reagent carrier and a shell module, wherein the control center is connected with the magnetic suction module, the gun arranging module, the reagent carrier and the shell module; the rifle arranging module comprises a suction and discharge structure and a moving module, and a liquid suction structure and a liquid discharge structure are arranged in the suction and discharge structure; the mobile module is connected with a motor. The magnetic attraction module is used for attracting carboxyl magnetic beads, and the carboxyl magnetic beads are common substances in a magnetic bead coupling method. The reagent carrier is used for supporting the reagent strip and positioning the reagent strip. The control center is used for scheduling the actions of the magnetic suction module, the gun arranging module and the reagent carrier during detection so as to cooperate with the detection steps.

Optionally, the magnets of the magnetic starting module are uniformly distributed on the working surface of the magnetic module. The module is inhaled to magnetism is equipped with the recess that corresponds with the reagent strip, and the working face is the medial surface of recess.

Optionally, the electromagnet is connected with a power supply, and the power supply is electrically connected with the control center.

Optionally, the suction structure is provided with a piston and a driving structure, the driving structure is provided with a cylinder or a motor connected with the piston, and a suction head is connected below the piston in a sealing and sliding manner. The number of the waste pipette tip positions and the number of the new pipette tip positions are the same as the number of the reagent carriers and are opposite to each other. The array gun module is provided with a suction head, a movable tool and a suction row driving structure, the movable tool and the suction row driving structure are connected to the control system, and the suction head is inserted into a reagent or sample hole of the reagent strip to move.

Optionally, a sliding rail structure or a gear transmission structure is arranged in the moving module.

Optionally, a calibration structure is arranged in the gun arranging module, the calibration structure is provided with a circular bulge, and a lifting structure is arranged on the circular bulge and connected to the control center. Be equipped with the round hole that is used for the location on the reagent strip, circular arch and round hole cooperation can be with the reagent strip location and with its spacing fixed in testing process.

Optionally, the housing module is provided with a transparent arc cover, the rotating shaft of the arc cover is connected to the housing and the motor, and the motor is connected to the control center. The arc cover can use the pivot as the rotation of axes, avoids external contamination among the testing process.

Optionally, a display screen is arranged outside the control center. The display screen can help the staff conveniently operate the device.

3. Advantageous effects

Compared with the prior art, the technical scheme provided by the utility model has the following beneficial effects:

(1) the metabolic small molecule sample detection system provided by the embodiment of the application has a clear structure and is convenient to operate.

(2) The metabolic small molecule sample detection system provided by the embodiment of the application has the advantages that the sample pretreatment operation is efficient and rapid.

(3) The metabolic small molecule sample detection system provided by the embodiment of the application is adaptive to an automatic detection device and good in compatibility.

Drawings

FIG. 1 is a block diagram of a method for detecting a small molecule sample according to an embodiment of the present invention.

FIG. 2 is a top view of a metabolic small molecule sample detection system according to an embodiment of the present invention.

1. A magnetic module; 2. a gun arranging module; 3. a reagent carrier; 4. a waste pipette tip position; 5. a new suction head position; 6. a work bench.

Detailed Description

For a further understanding of the present invention, reference will now be made in detail to the embodiments illustrated in the drawings.

The present application will be described in further detail with reference to the following drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the relevant invention and are not limiting of the utility model. It should be noted that, for convenience of description, only the portions related to the present invention are shown in the drawings. The terms first, second, and the like in the present invention are provided for convenience of describing the technical solution of the present invention, and have no specific limiting effect, but are all generic terms, and do not limit the technical solution of the present invention. It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are intended to be inclusive and mean, for example, that they may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art. The technical solutions in the same embodiment and the technical solutions in different embodiments can be arranged and combined to form a new technical solution without contradiction or conflict, and the technical solutions are within the scope of the present invention.

Examples

With reference to fig. 1-2, a metabolic small molecule sample detection system includes a control center, a magnetic module 1, a gun module 2, a reagent carrier 3 and a housing module, in this embodiment, an aldosterone antibody and a carboxyl magnetic bead are coupled to serve as a carrier for separating aldosterone in serum, an automated pretreatment instrument and a reagent strip are used for sample pretreatment, and then determination of aldosterone concentration in serum is achieved by liquid chromatography tandem mass spectrometry. The advantages of an immunoassay method and a liquid chromatography tandem mass spectrometry method are combined, an operation interface is visual and easy to use, the time required by the whole process is short, the problem that the clinical mass spectrometry detection is difficult to realize automation is solved, and the repeatability and the test accuracy of instrument detection are improved.

The control center is connected with the magnetic suction module 1, the row gun module 2, the reagent carrier frame 3 and the shell module, the shell module is provided with a transparent arc cover, the rotating shaft of the arc cover is connected with the shell and the motor, and the motor is connected with the control center. The arc cover can use the pivot as the rotation of axes, avoids external contamination among the testing process.

Control software is arranged in the control center, and a display screen is arranged outside the control center. The display screen can help the staff conveniently operate the device.

The magnetic module 1 comprises a magnetic starting module and a moving module, and an electromagnet is arranged in the magnetic starting module. And magnets of the magnetic starting module are uniformly distributed on the working surface of the magnetic module 1. The module 1 is inhaled to magnetism is equipped with the recess that corresponds with the reagent strip, and the working face is the medial surface of recess. The electromagnet is connected with a power supply, and the power supply is electrically connected with the control center.

The rifle arranging module 2 comprises a suction and discharge structure and a moving module, and a liquid suction structure and a liquid discharge structure are arranged in the suction and discharge structure. The suction structure is provided with a piston and a driving structure, the driving structure is provided with a cylinder or a motor connected with the piston, and a suction head is connected below the piston in a sealing and sliding manner. The number of the waste tip sites 4 and the number of the new tip sites 5 are the same as the number of the reagent carriers 3 and are opposite to each other. Be equipped with suction head, removal frock and suction row drive structure on row rifle module 2, remove frock and suction row drive structure and connect in control system, the suction head is gone into in reagent or the sample hole of reagent strip and is carried out the action. Be equipped with calibration structure in the rifle module 2, calibration structure is equipped with circular arch, be equipped with elevation structure on the circular arch and connect in control center. Be equipped with the round hole that is used for the location on the reagent strip, circular arch and round hole cooperation can be with the reagent strip location and with its spacing fixed in testing process.

The mobile module is connected with a motor, and a sliding rail structure or a gear transmission structure is arranged in the mobile module.

The magnetic module 1 is used for attracting carboxyl magnetic beads, and the carboxyl magnetic beads are common substances in a magnetic bead coupling method. The reagent carrier 3 is used for holding reagent strips and positioning the reagent strips. The control center is used for scheduling the actions of the magnetic suction module 1, the spray gun module 2 and the reagent carrier 3 during detection so as to cooperate with the detection steps.

The work table 6 is the entire platform, and all actions are completed on the work table 6.

The reagent strip is in a strip shape, the right side of the reagent strip is provided with a handle, and the reagent strip is a sample containing device with 15 holes.

The working steps are as follows:

1. and adding the required reagent and the sample into the corresponding sample hole of the reagent strip, and then completing the 2 nd to 4 th operation steps by using an automatic pretreatment instrument.

2. And (3) manually adding 50 mu L of 1mg/mL antibody coupled magnetic beads, 100 mu L of serum or a marking line and 50 mu L of internal standard into a seventh hole, incubating for 10min, blowing and beating for 5 times every 2min, and uniformly mixing, wherein the blowing and beating action is carried out by adopting a row gun module 22.

3. 200 mu LPBS is added into the third hole, the fourth hole and the fifth hole respectively, and the wells are washed for 1 time after incubation.

4. 200 μ L of 40% methanol was added to the thirteenth well, after washing, 100 μ L of 40% methanol was aspirated, and the hormone on the antibody magnetic beads was eluted by pipetting several times in 14 wells.

5. And taking 90 mu L of sample from the fourteenth hole to a 96-hole plate or a sample, and detecting on a Waters TQS mass spectrometer.

The following are some experimental test data:

the present invention and its embodiments have been described above schematically, without limitation, and what is shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. Therefore, if the person skilled in the art receives the teaching, without departing from the spirit of the utility model, the person skilled in the art shall not inventively design the similar structural modes and embodiments to the technical solution, but shall fall within the scope of the utility model.

Claims (8)

1. A metabolic small molecule sample detection system is characterized by comprising a control center, a magnetic suction module, a gun arranging module, a reagent carrier and a shell module, wherein the control center is connected with the magnetic suction module, the gun arranging module, the reagent carrier and the shell module; the rifle arranging module comprises a suction and discharge structure and a moving module, and a liquid suction structure and a liquid discharge structure are arranged in the suction and discharge structure; the mobile module is connected with a motor.

2. The system of claim 1, wherein the magnets of the magnetic force actuation module are uniformly distributed on the working surface of the magnetic attraction module.

3. The metabolic small molecule sample detection system according to claim 1, wherein the electromagnet is connected with a power supply, and the power supply is electrically connected with a control center.

4. The metabolic small molecule sample detection system according to claim 1, wherein the suction and discharge structure is provided with a piston and a driving structure, the driving structure is provided with a cylinder or a motor connected with the piston, and a suction head is connected below the piston in a sealing and sliding manner.

5. The system for detecting the metabolic small molecule sample according to claim 1, wherein a slide rail structure or a gear transmission structure is arranged in the moving module.

6. The system for detecting the metabolic small molecule sample according to claim 1, wherein the rifle module is internally provided with a calibration structure, the calibration structure is provided with a circular protrusion, and the circular protrusion is provided with a lifting structure and connected to a control center.

7. The metabolic small molecule sample detection system according to claim 1, wherein the housing module is provided with a transparent arc cover, the arc cover is rotatably connected to the housing and a motor, and the motor is connected to the control center.

8. The metabolic small molecule sample detection system according to any one of claims 1 to 6, wherein a display screen is arranged outside the control center.

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