CN215593075U

CN215593075U - Continuous screening device for soil microbial strains

Info

Publication number: CN215593075U
Application number: CN202121452784.3U
Authority: CN
Inventors: 朱治强; 王柏洁; 吴紫晗; 李媛
Original assignee: Hainan University
Current assignee: Hainan University
Priority date: 2021-06-29
Filing date: 2021-06-29
Publication date: 2022-01-21
Anticipated expiration: 2031-06-29

Abstract

The utility model discloses a continuous screening device for soil microbial strains, which comprises: screening outer tank, screening inner tank, microorganism sample jar and nutrient solution recovery tank, be equipped with the vacuum intermediate layer between screening outer tank and the screening inner tank, be fixed with on the screening outer tank and stretch into the screening inner tank carries out the agitating unit that stirs, the inboard symmetry in lower part of screening inner tank is fixed with the constant temperature heating pipe, one side of screening inner tank is equipped with the air inlet unit with external intercommunication, the top of screening inner tank is equipped with the air outlet means with external intercommunication, the inside of screening inner tank is equipped with the fungus liquid pipe, the screening inner tank with be equipped with the catheter in the vacuum intermediate layer between the bottom of screening outer tank, the one end and the fungus liquid pipe intercommunication of catheter, the other end with microorganism sample jar intercommunication, the catheter is provided with microorganism sieve screen with the kneck that the microorganism drawed jar. The device has controllable culture temperature, high screening efficiency and recyclable nutrient solution.

Description

Continuous screening device for soil microbial strains

Technical Field

The utility model belongs to the technical field of soil microorganism screening, and relates to a continuous screening device for soil microorganism strains.

Background

With the modern development of China, a series of environmental problems are generated, wherein the soil pollution problem is one of typical problems, the mature polluted soil restoration technology developed in China can be generally divided into physical restoration, chemical restoration and biological restoration, and the biological restoration technology is a newly developed technology with high benefit, low cost and great development potential. Wherein, the screening of soil microorganisms is a key link for the development of bioremediation technology.

The strain is a microorganism used as a living cell catalyst in the fermentation process, comprises four categories of bacteria, actinomycetes, saccharomycetes and mould, is derived from soil microorganisms, is separated and screened from soil to obtain a useful strain, is improved and is stored for being used for producing a microbial agent.

However, the existing culture and screening devices still have certain disadvantages, mainly including:

1. the culture temperature cannot be controlled in the screening system, the growth of microorganisms is not facilitated, and the screening efficiency is low.

2. The screening system has no aeration equipment, cannot provide oxygen for the cultured microorganisms, cannot discharge generated harmful gas, and is not beneficial to the culture of the microorganisms.

3. Nutrient solution in the screening system can not be recycled, which causes waste of experimental reagent materials.

SUMMERY OF THE UTILITY MODEL

The utility model provides a continuous screening device for soil microbial strains, which aims to solve the problems in the background technology.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a continuous screening device for soil microbial strains comprises: the microorganism nutrient solution recovery device comprises a screening box, a microorganism sampling tank and a nutrient solution recovery tank, wherein the screening box comprises an outer screening tank and an inner screening tank, a vacuum interlayer is arranged between the outer screening tank and the inner screening tank, a stirring device capable of extending into the inner screening tank for stirring is fixed on the outer screening tank, constant-temperature heating pipes are symmetrically fixed on the inner side of the lower part of the inner screening tank, an air inlet device communicated with the outside is arranged on one side of the inner screening tank, an air outlet device communicated with the outside is arranged at the top of the inner screening tank, a bacteria liquid guide pipe is arranged in the inner screening tank, a liquid guide pipe is arranged in the vacuum interlayer between the inner screening tank and the bottom of the outer screening tank, one end of the liquid guide pipe is communicated with the bacteria liquid guide pipe, the other end of the liquid guide pipe is communicated with the microorganism sampling tank, a microorganism screening net is arranged at the interface of the liquid guide pipe and the microorganism sampling tank, the nutrient solution recovery tank is adjacent to the microorganism sampling tank and is separated by a vertical plate, and a microorganism filter screen is arranged at the lower part of the vertical plate.

Preferably, the air inlet device comprises an air inlet pipe, a blower and an air guide pipe, the air inlet end of the blower is provided with the air inlet pipe, the outer wall of the air inlet pipe is in threaded connection with a threaded cover, a sealing film is clamped between the threaded cover and the air inlet pipe, and the outer wall of the top of the threaded cover is provided with a plurality of air holes; one end of the air duct is connected with the air outlet end of the air blower, and the other end of the air duct is communicated with the screening inner tank.

Preferably, the air outlet device comprises an air exchange pipe, a sleeve, a sliding rod and a movable plate, one end of the air exchange pipe is communicated with the screening inner tank, the other end of the air exchange pipe extends out of the screening outer tank, the sleeve is arranged at the top of the screening outer tank and located on the outer side of the air exchange pipe, a spring is fixed at the bottom of the sleeve, one end of the sliding rod is fixedly connected with the movable plate, the other end of the sliding rod is connected with the movable end of the spring to move up and down in the sleeve, a rubber pad is arranged at the bottom of the movable plate, and the rubber pad can cover an opening in the top end of the air exchange pipe.

Preferably, agitating unit includes agitator motor, transmission main shaft and stirring rake, agitator motor is fixed in the top center of screening outer jar, agitator motor with the transmission main shaft rotates to be connected, the outside of transmission main shaft is fixed with the stirring rake, the stirring rake is located the inside of screening inner tank.

More preferably, the number of the stirring paddles is not less than three.

Preferably, the number of the constant-temperature heating pipes is two, and the constant-temperature heating pipes are of a U-shaped structure.

Preferably, a feeding port is formed in one side of the top of the screening outer tank, a discharging port is formed in one side of the bottom of the screening outer tank, and the feeding port and the discharging port are communicated with the screening inner tank.

Preferably, the outer side of the bacteria liquid guide pipe is embedded and provided with an overflow hole.

Preferably, a bacteria liquid discharge port is formed in the liquid guide pipe, and a switch is installed on the bacteria liquid discharge port.

Preferably, the bottom of microorganism extraction jar is equipped with the microorganism and collects the mouth, the bottom of nutrient solution recovery jar is equipped with the nutrient solution and collects the mouth.

The utility model has the following beneficial effects:

1. through the constant temperature heating pipe that sets up, drive the high-speed rotation of stirring rake at agitator motor, carry out the in-process of intensive mixing to soil sample and nutrient solution, carry out the constant temperature heating to cultivateing the system, control cultivation temperature to be favorable to the microorganism to grow, improve the microbial activity for the screening process.

2. Through intake pipe, air-blower, screw cap, air duct and the sealing membrane that sets up, the work of drive air-blower can supply the microbial community to absorb in the leading-in screening box of outside air to accelerate the reproduction rate of microorganism, the sealing membrane that lies in between screw cap and the intake pipe simultaneously can effectively prevent other impurity entering screening box in the air, is favorable to the microbial cultivation more, and the result of use is better.

3. Through scavenge pipe, sleeve, spring, slide bar, fly leaf and the rubber pad that sets up, the gas in the screening incasement can be through scavenge pipe with fly leaf jack-up and discharge outside the screening incasement, when the screening incasement does not have gaseous production, the spring can stimulate the slide bar downwards, the slide bar stimulates the fly leaf downwards for rubber pad on the fly leaf supports the scavenge pipe, effectively avoids external debris to get into in the screening case, protects the thallus not contaminated, has further improved the result of use of device.

4. Through the microorganism sample jar that sets up and nutrient solution recovery tank, microorganism and nutrient solution in the screening case can enter into microorganism sample jar through the microorganism screen cloth, then, the nutrient solution in the microorganism sample jar can get into the nutrient solution recovery tank through the microorganism filter screen to make the nutrient solution of retrieving can recycle.

5. The microorganism and the nutrient solution in the bacteria liquid are separated through the arranged microorganism screening net and the microorganism filtering net, and the nutrient solution can be continuously recycled, so that the waste of experimental reagents is effectively reduced.

Drawings

FIG. 1 is a schematic structural diagram of a continuous screening apparatus for microbial strains according to the present application;

FIG. 2 is an enlarged schematic view of the structure at A in FIG. 1;

FIG. 3 is an enlarged view of the structure at B in FIG. 1;

in the figure: 1. screening the outer tank; 2. screening the inner tank; 3. a ventilation tube; 4. a discharge outlet; 5. a stirring motor; 6. Rotating the main shaft; 7. a stirring paddle; 8. a constant temperature heating pipe; 9. a bacteria liquid conduit 90 and an overflow hole; 10. a feeding port; 11. an air duct; 12. a blower; 13. a catheter; 14. a microorganism sampling tank; 15. a microorganism screening net; 16. a microorganism collection port; 17. a nutrient solution recovery tank; 18. a microorganism filter screen; 19. a nutrient solution collection port; 20. vacuum interlayer; 21. an air inlet pipe; 22. a threaded cap; 23. air holes are formed; 24. sealing films; 25. A sleeve; 26. a spring; 27. a slide bar; 28. a movable plate; 29. a rubber pad; 30. discharging a bacterium liquid; 31. a riser.

Detailed Description

In order to more clearly illustrate the present invention, the present invention will be described in further detail below with reference to examples and the accompanying drawings. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the utility model.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, and integral connections; they may be directly connected to each other, indirectly connected to each other through an intermediate member, or connected to each other through the inside of two members. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In addition, if no special description is given, all the components used in the following embodiments are existing components, and the corresponding connection manner thereof can also be implemented by conventional technical means, and will not be described in detail in this application.

Examples

Referring to fig. 1 to 3, the continuous screening apparatus for soil microbial strains provided in the present embodiment mainly includes a screening outer tank 1 and a screening inner tank 2, wherein both the screening outer tank 1 and the screening inner tank 2 are made of stainless steel members, and certainly, other environment-friendly materials can be selected; the interval has vacuum intermediate layer 20 between screening outer tank 1 and the screening inner tank 2, and in the screening process to the microbial strains, through vacuum intermediate layer 20's vacuum interval, can avoid the influence that external environmental factor produced the bacterial, ensures that the bacterial is in good screening environment.

The feed inlet 10 has been seted up to the top of screening outer jar 1 in right side position department, and the bin outlet 4 has been seted up to the below of screening outer jar 1 in left side position department. The top of screening outer tank 1 is located middle part position department and is fixed with agitator motor 5, and agitator motor 5 rotates and is connected with transmission main shaft 6, and the outside of transmission main shaft 6 is fixed with stirring rake 7, and three groups are no less than to the quantity of stirring rake 7.

The inboard of screening inner tank 2 is located lower extreme position department symmetry and is fixed with constant temperature heating pipe 8, and the quantity of constant temperature heating pipe 8 is two sets of, and constant temperature heating pipe 8 is the U-shaped structure, according to the space condition in the jar, also can select other suitable shapes. In screening process to the microbial species, add soil sample, nutrient solution to screening inner tank 2 inside through dog-house 10, and then agitator motor 5 work, drive transmission main shaft 6 and rotate, synchronous drive stirring rake 7 rotates, carries out even stirring to the nutrient solution and handles, avoids the nutrient solution phenomenon of layering that stews to appear, and 8 work of constant temperature heating pipe ensure that the system of cultivateing keeps constant temperature simultaneously to improve the activity of microorganism in the nutrient solution.

The left side outer wall of screening outer tank 1 has air-blower 12 through bolted connection, and the end of giving vent to anger of air-blower 12 is provided with air duct 11, and the top of screening outer tank 1 is located the left side position and is equipped with scavenge pipe 3, and scavenge pipe 3 is linked together with screening inner tank 2. Wherein, the air inlet end of the blower 12 is provided with an air inlet pipe 21, the outer wall of the air inlet pipe 21 is in threaded connection with a threaded cap 22, the outer wall of the top of the threaded cap 22 is provided with a plurality of air holes 23, and a sealing film 24 is clamped between the threaded cap 22 and the air inlet pipe 21; the outer wall of the left side of the upper portion of the screening outer tank 1 is welded with a sleeve 25, the inner wall of the bottom of the sleeve 25 is welded with a spring 26, the movable end of the top of the spring 26 is welded with a sliding rod 27, the top of the sliding rod 27 is welded with a movable plate 28, the bottom of the movable plate 28 is bonded with a rubber pad 29, the bottom of the rubber pad 29 is in contact with the top of the ventilation pipe 3, and the top opening of the ventilation pipe 3 can be covered. The stirring motor 5 and the blower 12 are both connected with a switch through wires, and the switch is connected with a power supply through wires.

2 bottom both sides of screening inner tank are connected with fungus liquid pipe 9, overflow hole 90 has been seted up in the outside embedding of fungus liquid pipe 9, and fungus liquid pipe 9 and catheter 13, fungus liquid run-off 30 is connected, to the misce bene of bacterial raw materials and nutrient solution and carry out the layering of stewing, after the layering finishes, open fungus liquid run-off 30's switch, and then the fungus liquid on the upper strata of stewing flows in from overflow hole 90 on fungus liquid pipe 9 and flows in microorganism sampling tank 14 through catheter 13, collect the back that finishes at the fungus liquid, open bin outlet 4, discharge fungus slag charge.

The fungus liquid pipe 9 links to each other with the catheter 13, the catheter 13 passes through in the vacuum intermediate layer 20 in the middle of screening outer tank 1 and the screening inner tank 2, connect in microorganism sample jar 14, the kneck is provided with microorganism screening net 15, the fungus liquid that flows in from overflow hole 90 on fungus liquid pipe 9, only microorganism and nutrient solution can flow in microorganism sample jar 14 through microorganism screening net 15, separate through riser 31 between microorganism sample jar 14 and the nutrient solution recovery jar 17, riser 31 bottom is provided with microorganism filter screen 18, only nutrient solution can flow in nutrient solution recovery jar 17 through microorganism filter screen 18 in the microorganism sample jar 14, collect microorganism and nutrient solution respectively from microorganism collection mouth 16 and nutrient solution collection mouth 19 at last, wherein, the nutrient solution of retrieving can reuse.

The working principle of the utility model is as follows: in the screening process of microbial strains, soil samples and nutrient solution are added into the screening inner tank 2 through the feed inlet 10, and then the stirring motor 5 works to drive the transmission main shaft 6 to rotate, the synchronous driving stirring paddle 7 rotates to uniformly stir the nutrient solution and the soil samples, and the phenomenon of standing and layering is avoided. Meanwhile, the constant-temperature heating pipe 8 works to ensure that the culture system keeps constant temperature. The blower 12 is driven to work, so that outside air can be introduced into the screening inner tank 2 to be absorbed by microorganism groups, the propagation rate of the microorganisms is accelerated, and the sealing film 24 positioned between the threaded cover 22 and the air inlet pipe 21 can effectively prevent other impurities in the air from entering the screening inner tank 2, so that the microorganism culture is facilitated, and the using effect is better; meanwhile, the movable plate 28 can be jacked up and discharged out of the screening outer tank 1 through the scavenging tube 3 by the gas in the screening inner tank 2, when no gas is generated in the screening inner tank 2, the spring 26 can downwards pull the sliding rod 27, the sliding rod 27 downwards pulls the movable plate 28, so that the rubber pad 29 on the movable plate 28 is propped against the scavenging tube 3, external sundries are effectively prevented from entering the screening inner tank 2, the thalli are protected from being polluted, and the using effect of the device is further improved. After the strain centrifugation is finished, the mixture of the strain raw material and the nutrient solution is subjected to standing layering, after the layering is finished, the switch of the strain liquid discharge port 30 is opened, then the strain liquid on the upper layer of the standing layer flows into the microorganism sampling tank 14 from the overflow hole 90 on the strain liquid guide pipe 9 through the liquid guide pipe 13, and after the strain liquid collection is finished, the discharge port 4 is opened, and strain slag is discharged. The nutrient solution and the microorganisms flowing in from the overflow hole 90 on the bacteria liquid conduit 9 can flow into the microorganism sampling tank 14 through the microorganism screening net 15, and simultaneously the nutrient solution flows into the nutrient solution recovery tank 17 through the microorganism screening net 18 to be collected for continuous recycling.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the scope of the present invention, and the technical solutions and the utility model concepts of the present invention are equivalent to or changed within the scope of the present invention.

Claims

1. A soil microbial strain continuous screening device is characterized by comprising: the device comprises a screening outer tank, a screening inner tank, a microorganism sampling tank and a nutrient solution recovery tank, wherein a vacuum interlayer is arranged between the screening outer tank and the screening inner tank, a stirring device capable of extending into the screening inner tank for stirring is fixed on the screening outer tank, constant-temperature heating pipes are symmetrically fixed on the inner side of the lower part of the screening inner tank, an air inlet device communicated with the outside is arranged on one side of the screening inner tank, an air outlet device communicated with the outside is arranged at the top of the screening inner tank, a bacteria liquid guide pipe is arranged in the screening inner tank, a liquid guide pipe is arranged in the vacuum interlayer between the screening inner tank and the bottom of the screening outer tank, one end of the liquid guide pipe is communicated with the bacteria liquid guide pipe, the other end of the liquid guide pipe is communicated with the microorganism sampling tank, a microorganism screening net is arranged at the interface of the liquid guide pipe and the microorganism extraction tank, and the nutrient solution recovery tank is adjacently separated from the microorganism sampling tank through a vertical plate, and a microorganism filter screen is arranged at the lower part of the vertical plate.

2. The continuous screening device for soil microbial strains according to claim 1, wherein the air inlet device comprises an air inlet pipe, an air blower and an air guide pipe, the air inlet end of the air blower is provided with the air inlet pipe, the outer wall of the air inlet pipe is in threaded connection with a threaded cover, a sealing film is clamped between the threaded cover and the air inlet pipe, and the outer wall of the top of the threaded cover is provided with a plurality of air holes; one end of the air duct is connected with the air outlet end of the air blower, and the other end of the air duct is communicated with the screening inner tank.

3. The continuous soil microorganism strain screening device according to claim 1 or 2, wherein the gas outlet device comprises a gas exchange pipe, a sleeve, a sliding rod and a movable plate, one end of the gas exchange pipe is communicated with the screening inner tank, the other end of the gas exchange pipe extends out of the screening outer tank, the sleeve is arranged on the top of the screening outer tank and is positioned on the outer side of the gas exchange pipe, a spring is fixed on the bottom of the sleeve, one end of the sliding rod is fixedly connected with the movable plate, the other end of the sliding rod is connected with the movable end of the spring so as to move up and down in the sleeve, and a rubber pad is arranged on the bottom of the movable plate and can cover the top opening of the gas exchange pipe.

4. The continuous screening device for soil microbial strains according to claim 1, wherein the stirring device comprises a stirring motor, a transmission main shaft and a stirring paddle, the stirring motor is fixed at the center of the top of the screening outer tank, the stirring motor is rotationally connected with the transmission main shaft, the stirring paddle is fixed on the outer side of the transmission main shaft, and the stirring paddle is positioned inside the screening inner tank.

5. A continuous soil microbial strain screening apparatus according to claim 4, wherein the number of said paddles is not less than three.

6. The continuous screening device for soil microbial strains according to claim 1 or 5, wherein the number of the constant-temperature heating pipes is two, and the constant-temperature heating pipes are of a U-shaped structure.

7. The continuous screening device of soil microbial strains according to claim 1, wherein a feeding port is formed at one side of the top of the screening outer tank, a discharging port is formed at one side of the bottom of the screening outer tank, and the feeding port and the discharging port are both communicated with the screening inner tank.

8. The continuous screening device of soil microbial strains according to claim 1, wherein an overflow hole is formed in the outer side of the bacteria liquid guide pipe in an embedded manner.

9. The continuous screening device for soil microbial strains according to claim 1 or 8, wherein the liquid guide tube is provided with a bacteria liquid discharge port, and the bacteria liquid discharge port is provided with a switch.

10. The continuous screening device for soil microbial strains according to claim 1, wherein the bottom of the microorganism extracting tank is provided with a microorganism collecting port, and the bottom of the nutrient solution recovery tank is provided with a nutrient solution collecting port.