CN215535305U

CN215535305U - PDX model tumor specimen implantation needle

Info

Publication number: CN215535305U
Application number: CN202122067919.0U
Authority: CN
Inventors: 章康健; 曹雪萍; 陈若愚
Original assignee: Shanghai Yuansong Biotechnology Co ltd
Current assignee: Shanghai Yuansong Biotechnology Co ltd
Priority date: 2021-08-30
Filing date: 2021-08-30
Publication date: 2022-01-18
Anticipated expiration: 2031-08-30

Abstract

The utility model discloses a PDX model tumor specimen implantation needle, relating to the field of tissue inoculation devices and comprising an outer shell, the bottom end of the outer shell is connected with a connecting part, the bottom end of the connecting part is connected with a conical part, the bottom end of the conical part is connected with an outer sleeving needle tube, one side of the outer sleeving needle tube is provided with a side hole, an inner sleeve is fixedly arranged inside the outer shell, the inner side of the inner sleeve is provided with an internal thread, a pushing assembly is arranged in the outer shell, the outer sleeving needle tube and the connecting rod are provided with scales, and the inoculation of the tissue block can be controlled more accurately by the pushing in a rotating mode, so that the accurate inoculation is ensured, the tumor tissue is placed into the inner wall of the externally sleeved needle tube through the side hole, and the mouse is punctured to an accurate subcutaneous position during the inoculation and then pushed for inoculation, so that the completeness of the inoculated tumor tissue is ensured, the problem of low inoculation amount is solved, and the falling of cancer cells and tissues caused in the puncturing process is also avoided.

Description

PDX model tumor specimen implantation needle

Technical Field

The utility model relates to the field of tissue inoculation devices, in particular to a PDX model tumor specimen implantation needle.

Background

The establishment of tumor models using mice is an important approach in modern oncology research. Mouse tumor models can be divided into subcutaneous and in situ neoplasia depending on the site of inoculation. Because the subcutaneous tumor formation operation is simple and convenient to observe, and compared with the in-situ tumor formation, the pathological physiology of the tumor has no obvious difference, the application in practical research is more common. The PDX (patent-derivative tumor grafts) model is a very important model tool in the field of precise medical treatment, the PDX model refers to that fresh tumor tissues of a patient are simply treated and transplanted to an immunodeficient mouse, the fresh tumor tissues grow depending on the environment provided by the mouse, the model maintains the microenvironment and basic characteristics of primary tumors, and then the curative effect of a research drug is evaluated through mouse administration. The model provides a good in-vivo model for researching tumors. The conventional PDX model is constructed by the following steps: firstly, collecting tumor specimens of a patient, and completely soaking the tumor specimens in a 0 ℃ double-resistant PRMI1640 culture medium. ② the animal room is cleaned by 0 ℃ double-resistant PRMI1640 culture medium and then is completely soaked, and the animal room is carried back to the animal room for operation. (iii) tumor tissue was trimmed to 2 x 3mm3 and washed 3 times in the above medium. Under anesthesia, a small opening of about 3mm is respectively formed in the skin on the two sides of the subcutaneous part of a nude mouse with the age of 4-6 weeks, a small pocket space is separated, the trimmed tumor small tissue blocks are planted under the skin, and the skin cut is sutured. And dripping one drop of 100 double-resistant solution on the incision to prevent wound infection. Observing the condition of the implanted tumor once a week, and after about 3-5 weeks, the implanted tumor begins to grow to the size of 1-2cm 3.

At present, when a PDX model is reconstructed, the process of subcutaneous incision and suture of a nude mouse is complicated, and the operation risk is high, and a Chinese utility model 'tumor mass inoculator' (the publication number is 204016544U) discloses a tumor mass inoculator, but the inoculator has a complex structure, and has the problems of inaccurate inoculation, incomplete inoculated tumor tissues and low inoculation amount; in addition, when the puncture needle is used, the tumor mass is inserted from the needle head of the outer sleeve needle tube, so that the shedding of cancer cells and tissues in the puncture process can be caused, and the tumor grows dispersedly along the whole puncture channel and the puncture point.

SUMMERY OF THE UTILITY MODEL

The utility model provides a PDX model tumor specimen implantation needle, which solves the problems in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme:

the utility model provides a PDX model tumor specimen implants needle, which comprises an outer shell, the bottom of shell body is connected with connecting portion, the toper portion is connected to the bottom of connecting portion, the outer trocar tube is connected to the bottom of toper portion, the side opening has been seted up to one side of outer trocar tube, the inside of shell body is fixed and is equipped with inside cover, the inboard of inside cover is equipped with the internal thread, be equipped with the propelling movement subassembly in the shell body, the propelling movement subassembly includes the connecting rod, the one end of connecting rod extends to in the shell body and fixed connection screw thread seat, screw thread seat and inside cover threaded connection, the one end fixed connection propelling movement pole of connecting rod is kept away from to the screw thread seat, the propelling movement pole extends to in the outer trocar tube.

As a preferable technical scheme of the utility model, one end of the connecting rod, which is far away from the threaded seat, is fixedly connected with a rotating handle.

As a preferable technical scheme of the utility model, the outer part of the outer sleeve needle tube is provided with a first scale mark, and the outer part of the connecting rod is provided with a second scale mark.

As a preferred technical solution of the present invention, the top end and the bottom end of the inner sleeve are respectively and fixedly connected to the upper fixing base and the lower fixing base.

As a preferable technical scheme of the utility model, a first buckle is arranged on the outer side of the connecting part, and a second buckle matched and connected with the first buckle is arranged on the inner side of the bottom end of the inner sleeve.

The utility model has the following advantages: the scales are arranged on the outer trocar tube and the connecting rod, and the tissue block can be accurately controlled to be inoculated by pushing in a rotating mode, so that the accurate inoculation is ensured, the tumor tissue is placed into the inner wall of the outer trocar tube through the side hole, and the mouse is pushed and inoculated after being punctured to the subcutaneous accurate position during inoculation, so that the completeness of the inoculated tumor tissue is ensured, the problem of low inoculation amount is solved, and the falling of cancer cells and tissues caused in the puncturing process is avoided.

Drawings

Fig. 1 is a structural schematic diagram of a PDX model tumor specimen implantation needle.

Fig. 2 is a schematic structural diagram of a pushing assembly in a PDX model tumor specimen implantation needle.

Fig. 3 is a schematic structural diagram of the outer shell of the PDX model tumor specimen implantation needle.

Fig. 4 is a schematic structural diagram of a connecting part in a PDX model tumor specimen implantation needle.

In the figure: 1. an outer housing; 2. a tapered portion; 3. sleeving a needle tube outside; 4. a side hole; 5. a first scale mark; 6. a connecting rod; 7. rotating the handle; 8. a threaded seat; 9. a push rod; 10. an inner sleeve; 11. an upper fixed seat; 12. a lower fixed seat; 13. a first buckle; 14. a connecting portion; 15. a second buckle; 16. a second tick mark.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.

It should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

Example 1

Please refer to fig. 1-4, a PDX model tumor specimen implantation needle, comprising an outer casing 1, the bottom end of the outer casing 1 is connected with a connection portion 14, the bottom end of the connection portion 14 is connected with a taper portion 2, the bottom end of the taper portion 2 is connected with an outer thimble tube 3, one side of the outer thimble tube 3 is provided with a side hole 4, the inside of the outer casing 1 is fixedly provided with an inner sleeve 10, the inner side of the inner sleeve 10 is provided with an internal thread, the outer casing 1 is provided with a pushing assembly, the pushing assembly comprises a connection rod 6, one end of the connection rod 6 extends into the outer casing 1 and is fixedly connected with a threaded seat 8, the threaded seat 8 is in threaded connection with the inner sleeve 10, one end of the threaded seat 8 far away from the connection rod 6 is fixedly connected with a pushing rod 9, and the pushing rod 9 extends into the outer thimble tube 3.

And one end of the connecting rod 6, which is far away from the threaded seat 8, is fixedly connected with a rotating handle 7.

The outer part of the outer sleeve needle tube 3 is provided with a first scale mark 5, and the outer part of the connecting rod 6 is provided with a second scale mark 16.

The top end and the bottom end of the inner sleeve 10 are respectively and fixedly connected with an upper fixed seat 11 and a lower fixed seat 12.

The outer side of the connecting part 14 is provided with a first buckle 13, and the inner side of the bottom end of the inner sleeve 10 is provided with a second buckle 15 which is matched and connected with the first buckle 13.

In the implementation process of the utility model, one end of the outer thimble tube 3 far away from the conical part 2 is a tip surface, so that the resistance applied during inoculation is reduced, the side hole 4 is 1.5cm away from the tip surface, and the size of the side hole 4 is 2mm multiplied by 2mm and is used for being plugged into a tissue block; the first scale mark 5 outside the outer sleeve needle tube 3 can accurately judge the depth of the inserted needle,

the length of the pushing stem is 5mm more than that of the outer thimble tube 3, the lower edge of the pushing stem is a solid circular section, so that the tissue block can be conveniently pushed to a puncture point for a long distance and has small damage to the tissue of a mouse, and the pushing rod 9 can be accurately controlled to push the tissue block to the subcutaneous part of the mouse through rotation when the connecting rod 6 is rotated; the connecting rod 6 is provided with scales, and the scales can be accurately displayed when the connecting rod 6 is exposed out of the outer shell 1 through rotation, so that experimenters can clearly judge the progress pushed by the inner pushing rod 9; the connecting rod 6 and the threaded seat 8 can be driven to rotate by rotating the rotating handle 7, and the pushing rod 9 is pushed to be inserted into the cavity of the outer sleeve needle tube 3.

The specific use cases are as follows,

1. firstly, the connecting part 14 is connected with the outer shell 1 and the inner sleeve 10 through the first buckle 13 and the second buckle 15, so that the assembly is realized;

2. the rotating handle 7 is rotated to enable the scale of the connecting rod 6 extending out of the outer shell 1 to be 0, and at the moment, the threaded seat 8 is stably connected with the inner sleeve 10;

3. except for placing the tissue block into the inner wall of the outer sleeve needle tube 3 at the side hole 4;

4. the inoculation depth can be judged by the scale on the outer sleeve needle tube 3 after the inoculation to the subcutaneous part of the mouse;

5, the rotating handle 7 is rotated to enable the pushing rod 9 to be inserted into the cavity of the outer sleeve needle tube 3, the tissue block is pushed towards the subcutaneous tissue of the mouse, and whether the tissue block is pushed to the target position can be judged through the second scale marks 16 on the connecting rod 6.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the utility model. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a PDX model tumor specimen implants needle, which comprises an outer shell body, a serial communication port, the bottom of shell body is connected with connecting portion, the toper portion is connected to the bottom of connecting portion, the outer trocar tube is connected to the bottom of toper portion, the side opening has been seted up to one side of outer trocar tube, the inside of shell body is fixed and is equipped with inside cover, the inboard of inside cover is equipped with the internal thread, be equipped with the propelling movement subassembly in the shell body, the propelling movement subassembly includes the connecting rod, the one end of connecting rod extends to in the shell body and fixed connection screw thread seat, screw thread seat and inside cover threaded connection, the one end fixed connection propelling movement pole of connecting rod is kept away from to the screw thread seat, the propelling movement pole extends to in the outer trocar tube.

2. The PDX model tumor specimen implantation needle as defined in claim 1, wherein the end of the connection rod away from the threaded seat is fixedly connected to a rotation handle.

3. The PDX model tumor specimen implantation needle as defined in claim 1, wherein the outer portion of the outer cannula tube has a first graduation mark, and the outer portion of the connecting rod has a second graduation mark.

4. The PDX model tumor specimen implantation needle as defined in claim 1, wherein the top end and the bottom end of the inner sleeve are respectively and fixedly connected with the upper fixing seat and the lower fixing seat.

5. The PDX model tumor specimen implantation needle as claimed in claim 1, wherein the connecting portion is provided with a first buckle at the outer side thereof, and the inner sleeve is provided with a second buckle at the inner side of the bottom end thereof for engaging with the first buckle.