CN215492746U - Integrated pretreatment device - Google Patents
Integrated pretreatment device Download PDFInfo
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- CN215492746U CN215492746U CN202121757007.XU CN202121757007U CN215492746U CN 215492746 U CN215492746 U CN 215492746U CN 202121757007 U CN202121757007 U CN 202121757007U CN 215492746 U CN215492746 U CN 215492746U
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Abstract
The utility model discloses an integrated pretreatment device, which comprises a cylinder a, wherein one end of the cylinder a is opened, the outer wall of the opening end of the cylinder a is provided with outer pipe threads, the cylinder a is connected with a cylinder b, one end of the cylinder b is opened, the inner wall of the opening end of the cylinder b is provided with inner pipe threads, the cylinder a and the cylinder b are connected through the threads, a sieve plate insertion position a is arranged in the cylinder a, a sieve plate a is inserted in the sieve plate insertion position a, a sieve plate insertion position b and a sieve plate insertion position c are arranged in the cylinder b, a sieve plate b is inserted in the sieve plate insertion position b, a sieve plate c is inserted in the sieve plate insertion position c, a filler a is filled between the sieve plate b and the sieve plate c, a liquid outlet is arranged in the middle of the other end surface of the cylinder a, and an injector or a plug is arranged in the liquid outlet. The integrated pretreatment device has the advantages of simple structure and convenient use, and can realize the integrated process of sample extraction, purification and filtration in the device.
Description
Technical Field
The utility model relates to the technical field of analysis and detection devices, in particular to an integrated pretreatment device based on QuEChERS.
Background
QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) is a rapid sample pretreatment technology newly developed internationally in recent years, developed by the American academy of agriculture in 2003 and characterized in that: fast, simple, cheap, efficient, durable and safe; the main principle is that the adsorbent filler and impurities in the matrix are interacted to adsorb the impurities so as to achieve the purpose of purification.
The QuEChERS method generally comprises the steps of firstly moving a sample extracting solution into a centrifuge tube, adding a QuEChERS purifying agent, then carrying out vortex centrifugation, frequently filtering obtained supernatant to remove tiny particles, and then carrying out analysis by a sample chromatography-mass spectrometry system, wherein the defects in practical application are as follows: the extraction, purification, filtration of sample need utilize different containers and material to go on step by step, need centrifugal equipment simultaneously, lead to the operation tedious consuming time, and solution transfer process can make the experimenter expose in the chemical reagent environment, is unfavorable for personnel's health.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide an integrated pretreatment device, which can realize the integrated process of sample extraction, purification and filtration in the device.
In order to solve the technical problem, the integrated pretreatment device provided by the utility model comprises a cylinder a, one end of the cylinder a is open, the outer wall of the open end of the cylinder a is provided with an outer pipe thread, the cylinder a is connected with a cylinder b, one end of the cylinder b is open, the inner wall of the open end of the cylinder b is provided with an inner pipe thread, the cylinder a is connected with the cylinder b through a thread, a sieve plate insertion position a is arranged in the cylinder a, the sieve plate a is inserted in the sieve plate insertion position a, a sieve plate insertion position b and a sieve plate insertion position c are arranged in the cylinder b, the sieve plate b is inserted in the sieve plate insertion position b, the sieve plate c is inserted in the sieve plate insertion position c, a filler a is filled between the sieve plate b and the sieve plate c, and a liquid outlet is formed in the middle of the other end face of the cylinder a.
Specifically, a syringe or a plug is arranged in the liquid outlet.
Specifically, the open end of the column a is provided with a cover.
Specifically, still be provided with the sieve in the cylinder b and insert a position d, insert sieve d in the sieve inserts a position d, pack between sieve c and the sieve d and pack b.
Specifically, the diameter of cylinder b is slightly larger than that of cylinder a.
Preferably, the material of the column a is one of polypropylene, polyethylene and polycarbonate.
Preferably, the material of the cylinder b is one of polypropylene, polyethylene, polytetrafluoroethylene and glass.
Preferably, the materials of the sieve plate a, the sieve plate b, the sieve plate c and the sieve plate d are all one of polypropylene, polyethylene, polytetrafluoroethylene and glass cellulose.
The QueCHERS-based integrated pretreatment device can carry out the pretreatment process in the same device, the whole process is carried out in a closed manner, the device is simple, the operation is convenient, the working efficiency is improved, and the volatilization of harmful substances is avoided due to the closed process, so that the health of experimenters is facilitated.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the present invention are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of an integrated pretreatment apparatus according to the present invention;
FIG. 2 is a schematic diagram of the extraction and purification process in an integrated pretreatment apparatus according to the present invention;
FIG. 3 is a schematic view of a filtration process in an integrated pretreatment apparatus according to the present invention;
in the figure, 1, a column body a, 2, a column body b, 3, a sieve plate a, 4, a sieve plate inserting position a, 5, a liquid outlet, 6, a plug, 7, a sieve plate b, 8, a sieve plate c, 9, a sieve plate d, 10, a sieve plate inserting position b, 11, a sieve plate inserting position c, 12, a sieve plate inserting position d, 13, a filler a, 14, a filler b, 15, a cover and 16 are injectors.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The utility model relates to an integrated pretreatment device, which comprises a column a1, wherein one end of the column a1 is opened, the outer wall of the opening end of the column a is provided with an outer pipe thread, the column a1 is connected with a column b2, one end of the column b2 is opened, the inner wall of the opening end of the column b2 is provided with an inner pipe thread, the column a1 is in threaded connection with the column b2, a sieve plate insertion position a4 is arranged in the column a1, a sieve plate a3 is inserted into a sieve plate insertion position a4, a sieve plate insertion position b10 and a sieve plate insertion position c11 are arranged in the column b2, a sieve plate b7 is inserted into a sieve plate insertion position b10, a sieve plate c8 is inserted into a sieve plate insertion position c11, a filler a13 is filled between the sieve plate b7 and the sieve plate c8, and an outlet 5 is arranged in the middle of the other end face of the column a 1.
The open end of cylinder a1 can also be sealed by fitting a conventional plastic centrifuge tube cap.
The cylinder a1 is made of one of polypropylene, polyethylene and polycarbonate; the cylinder b2 is made of one of polypropylene, polyethylene, polytetrafluoroethylene and glass; the sieve plate a3, the sieve plate b7, the sieve plate c8 and the sieve plate d9 are made of one of polypropylene, polyethylene, polytetrafluoroethylene and glass cellulose; the aperture of the sieve plate a3 is 50-200um, and the further optimization is 20-100 um; the aperture of the sieve plate b7, the sieve plate c8 and the sieve plate d9 is 10um-20 um.
Example one
In the integrated pretreatment apparatus of the present invention, as shown in FIG. 1, a liquid outlet 5 at the lower part of a column a1 is blocked by a stopper 6 to prevent leakage between the liquid outlet 5 and the stopper 6, and the column a1 is made of polypropylene and has a specification of 50 mL.
As shown in fig. 2, the sample is moved into a column a1, an extraction solvent is added, extraction is performed by a homogenizer or other instruments/methods, after extraction is finished, a QueChERS reagent (such as anhydrous magnesium sulfate, C18, PSA and the like) is added to remove sample matrix interference in the extraction solvent, a cover 15 is covered, and the sample is mixed and purified (by hand shaking or other methods) for 1-2 min;
as shown in fig. 3, after the purification is finished, the cover 15 is opened, the sieve plate a3 is installed in the sieve plate insertion position a4 of the column a1, the aperture of the sieve plate a3 is 100um, the thickness is 2mm, the sieve plate b7, the sieve plate c8 and the sieve plate d9 are respectively installed in the sieve plate insertion position b10, the sieve plate insertion position c11 and the sieve plate insertion position d12 of the column b2, a filler a13 (a microporous filter membrane) is filled between the sieve plate b7 and the sieve plate c8, and another filler b14 (such as anhydrous sodium sulfate) is filled between the sieve plate c8 and the sieve plate d 9; mounting the cylinder b2 on the cylinder a1, wherein the cylinder b2 is an SPE type cylinder made of polypropylene (PP), the diameter of the cylinder b2 is slightly larger than that of the cylinder a1, and the cylinder b 3578 is matched with the cylinder a1 for use; and (3) turning over the device, removing a plug 6 at the lower part of the cylinder a1 for blocking the liquid outlet 5, inserting the adapted head-removing syringe 16 into the liquid outlet 5 of the cylinder a1 for pressurization, enabling the liquid to sequentially pass through a sieve plate a3, a sieve plate d9, a filler b14 (such as anhydrous sodium sulfate), a sieve plate c8, a filler a13 (a microporous filter membrane layer) and a sieve plate b7, and finally collecting the sample solution to be analyzed.
Example two
In the integrated pretreatment apparatus of the present invention, as shown in FIG. 1, a liquid outlet 5 at the lower part of a column a1 is blocked by a stopper 6 to prevent leakage between the liquid outlet 5 and the stopper 6, and the column a1 is made of polypropylene and has a specification of 50 mL.
As shown in fig. 2, the sample is moved into a column a1, an extraction solvent is added, extraction is performed by a homogenizer or other instruments/methods, after extraction is finished, a QueChERS reagent (such as anhydrous magnesium sulfate, C18, PSA and the like) is added to remove sample matrix interference in the extraction solvent, a cover 15 is covered, and the sample is mixed and purified (by hand shaking or other methods) for 1-2 min;
as shown in fig. 3, after the purification is finished, the cover 15 is opened, the sieve plate a3 is installed in the sieve plate insertion position a4 of the column a1, the aperture of the sieve plate a3 is 100um, the thickness is 2mm, the sieve plate b7 and the sieve plate c8 are respectively installed in the sieve plate insertion position b10 and the sieve plate insertion position c11 of the column b2, and a filler a13 (microporous filter membrane) is filled between the sieve plate b7 and the sieve plate c 8; mounting the cylinder b2 on the cylinder a1, wherein the cylinder b2 is an SPE type cylinder made of polypropylene (PP), the diameter of the cylinder b2 is slightly larger than that of the cylinder a1, and the cylinder b 3578 is matched with the cylinder a1 for use; and (3) turning over the device, removing the plug 6 at the lower part of the cylinder a1 for blocking the liquid outlet 5, inserting the adapted head-removing syringe 16 into the liquid outlet 5 of the cylinder a1 for pressurization, enabling the liquid to sequentially pass through the sieve plate a3, the sieve plate c8, the filler a13 (a microporous filter membrane layer) and the sieve plate b7, and finally collecting the sample solution to be analyzed.
EXAMPLE III
In the integrated pretreatment apparatus of the present invention, as shown in FIG. 1, a liquid outlet 5 at the lower part of a column a1 is blocked by a stopper 6 to prevent leakage between the liquid outlet 5 and the stopper 6, and the column a1 is made of polypropylene and has a specification of 50 mL.
As shown in fig. 2, the sample is moved into a column a1, an extraction solvent is added, extraction is performed by a homogenizer or other instruments/methods, after extraction is finished, a QueChERS reagent (such as anhydrous magnesium sulfate, C18, PSA and the like) is added to remove sample matrix interference in the extraction solvent, a cover 15 is covered, and the sample is mixed and purified (by hand shaking or other methods) for 1-2 min;
as shown in fig. 3, after the purification is finished, the cover 15 is opened, the sieve plate a3 is installed in the sieve plate insertion position a4 of the column a1, the aperture of the sieve plate a3 is 100um, the thickness is 2mm, the sieve plate b7, the sieve plate c8 and the sieve plate d9 are respectively installed in the sieve plate insertion position b10, the sieve plate insertion position c11 and the sieve plate insertion position d12 of the column b2, a filler a13 (such as anhydrous sodium sulfate) is filled between the sieve plate b7 and the sieve plate c8, and another filler b14 (a microporous filter) is filled between the sieve plate c8 and the sieve plate d 9; mounting the cylinder b2 on the cylinder a1, wherein the cylinder b2 is an SPE type cylinder made of polypropylene (PP), the diameter of the cylinder b2 is slightly larger than that of the cylinder a1, and the cylinder b 3578 is matched with the cylinder a1 for use; and (3) turning over the device, removing a plug 6 at the lower part of the cylinder a1 for plugging the liquid outlet 5, inserting the adapted head-removing syringe 16 into the liquid outlet 5 of the cylinder a1 for pressurization, enabling the liquid to sequentially pass through a sieve plate a3, a sieve plate d9, a filler b14 (a microporous filter membrane), a sieve plate c8, a filler a13 (such as anhydrous sodium sulfate) and a sieve plate b7, and finally collecting a sample solution to be analyzed.
Example four
In the integrated pretreatment apparatus of the present invention, as shown in FIG. 1, a liquid outlet 5 at the lower part of a column a1 is blocked by a stopper 6 to prevent leakage between the liquid outlet 5 and the stopper 6, and the column a1 is made of polypropylene and has a specification of 50 mL.
As shown in fig. 2, the sample is moved into a column a1, an extraction solvent is added, extraction is performed by a homogenizer or other instruments/methods, after extraction is finished, a QueChERS reagent (such as anhydrous magnesium sulfate, C18, PSA and the like) is added to remove sample matrix interference in the extraction solvent, a cover 15 is covered, and the sample is mixed and purified (by hand shaking or other methods) for 1-2 min;
as shown in fig. 3, after the purification is finished, the cover 15 is opened, the sieve plate a3 is installed in the sieve plate insertion position a4 of the column a1, the aperture of the sieve plate a3 is 100um, the thickness is 2mm, the sieve plate c8 and the sieve plate d9 are respectively installed in the sieve plate insertion position c11 and the sieve plate insertion position d12 of the column b2, and other fillers b14 (microporous filter membranes or other fillers) are filled between the sieve plate c8 and the sieve plate d 9; mounting the cylinder b2 on the cylinder a1, wherein the cylinder b2 is an SPE type cylinder made of polypropylene (PP), the diameter of the cylinder b2 is slightly larger than that of the cylinder a1, and the cylinder b 3578 is matched with the cylinder a1 for use; and (3) turning over the device, removing a plug 6 at the lower part of the cylinder a1 for plugging the liquid outlet 5, inserting the adapted head-removed syringe 16 into the liquid outlet 5 of the cylinder a1 for pressurization, allowing the liquid to pass through the sieve plate a3, the sieve plate d9, the filler b14 (microporous filter membrane or other fillers) and the sieve plate c8 in sequence, and finally collecting the sample solution to be analyzed.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (8)
1. An integrated pretreatment device is characterized by comprising a cylinder a (1), wherein one end of the cylinder a (1) is opened, the outer wall of the opening end of the cylinder a (1) is provided with an outer pipe thread, the cylinder a (1) is connected with a cylinder b (2), one end of the cylinder b (2) is open, the inner wall of the open end is provided with inner pipe threads, the column body a (1) is connected with the column body b (2) through screw threads, a sieve plate inserting position a (4) is arranged in the column body a (1), a sieve plate a (3) is inserted into the sieve plate inserting position a (4), a sieve plate inserting position b (10) and a sieve plate inserting position c (11) are arranged in the column b (2), a sieve plate b (7) is inserted into the sieve plate inserting position b (10), a sieve plate c (8) is inserted into the sieve plate inserting position c (11), a filler a (13) is filled between the sieve plate b (7) and the sieve plate c (8), and a liquid outlet (5) is arranged in the middle of the other end surface of the column body a (1).
2. An integrated pretreatment device according to claim 1, wherein a syringe (16) or a stopper (6) is provided in the liquid outlet (5).
3. An integrated pretreatment device according to claim 1, wherein the open end of the column a (1) is provided with a cover (15).
4. The integrated pretreatment device according to claim 1, wherein a sieve plate insertion position d (12) is further arranged in the column b (2), a sieve plate d (9) is inserted into the sieve plate insertion position d (12), and a filler b (14) is filled between the sieve plate c (8) and the sieve plate d (9).
5. The integrated pretreatment device according to claim 1, wherein the diameter of the column b (2) is slightly larger than that of the column a (1).
6. The integrated pretreatment device according to claim 1, wherein the column a (1) is made of one of polypropylene, polyethylene and polycarbonate.
7. The integrated pretreatment device according to claim 1, wherein the column b (2) is made of one of polypropylene, polyethylene, polytetrafluoroethylene and glass.
8. The integrated pretreatment device according to claim 1 or 4, wherein the sieve plate a (3), the sieve plate b (7), the sieve plate c (8) and the sieve plate d (9) are made of one of polypropylene, polyethylene, polytetrafluoroethylene and glass cellulose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121757007.XU CN215492746U (en) | 2021-07-30 | 2021-07-30 | Integrated pretreatment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121757007.XU CN215492746U (en) | 2021-07-30 | 2021-07-30 | Integrated pretreatment device |
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| Publication Number | Publication Date |
|---|---|
| CN215492746U true CN215492746U (en) | 2022-01-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121757007.XU Active CN215492746U (en) | 2021-07-30 | 2021-07-30 | Integrated pretreatment device |
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| CN (1) | CN215492746U (en) |
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2021
- 2021-07-30 CN CN202121757007.XU patent/CN215492746U/en active Active
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