CN215480965U - Cell constant temperature culture apparatus - Google Patents

Abstract

The utility model discloses a cell constant-temperature culture device which comprises a base, wherein a box body is arranged on one side of the top of the base, pulleys are arranged at four corners of the bottom of the base, sliding grooves are formed in two sides of the inner wall of the box body, the number of the sliding grooves is two, the number of the sliding grooves in each group is two, and a limiting mechanism is arranged between the two sliding grooves; the limiting mechanism comprises a fixing plate. According to the utility model, the limiting tightness of the cell culture dishes is effectively increased through the limiting mechanism, the effect of stable placement is achieved, multiple cells can be cultured simultaneously, mutual influence among the cell culture dishes is prevented, then the water in the liquid storage tank is conveyed to the liquid guide hose through the liquid guide pipe and the liquid guide pipe by arranging the water pump, nutrient solution is dripped into each cell culture dish in sequence, the influence on cell culture caused by external bacteria entering the box body is prevented, the cell culture quality is ensured, and the cell culture efficiency is improved.

Description

Cell constant temperature culture apparatus

Technical Field

The utility model relates to the technical field of cell culture, in particular to a constant-temperature cell culture device.

Background

Cell culture refers to a method for simulating in vivo environment in vitro to enable the cell to survive, grow, reproduce and maintain main structure and function, and is also called cell cloning technology, the formal term in biology is cell culture technology, and the cell culture is an essential process for whole bioengineering technology or one of the biotechnology, the cell culture is a large-scale cloning of cells, the cell culture technology is an important and common technology in cell biology research methods, a large number of cells can be obtained through cell culture, signal transduction of the cells, anabolism of the cells, growth and proliferation of the cells and the like can be researched, a culture medium and a culture box are needed for cell culture, and specific culture media and culture boxes are configured according to different types and life habits of the cells.

The existing cell culture box is flat placed in the constant-temperature culture box due to the structural reason, the stability of the existing cell culture box is difficult to guarantee, and nutrient solution cannot be added to cell strains in a culture bag, so that the nutrient solution needs to be added manually when added, the labor capacity is increased, the cells are easily damaged, the tightness of the culture box is insufficient due to the fact that water needs to be added in the culture process, and the cells in the culture box are easily infected by mixed bacteria in the environment.

Therefore, it is necessary to develop a cell culture apparatus at a constant temperature to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a cell constant-temperature culture device, which effectively increases the limiting tightness of cell culture dishes through a limiting mechanism, achieves the effect of stable placement, can simultaneously culture various cells, then conveys water in a liquid storage tank to a liquid guide hose through a liquid guide pipe and a liquid discharge pipe by arranging a water pump, and sequentially and independently drips nutrient solution for each cell culture dish, so as to solve the defects in the technology.

In order to achieve the above purpose, the utility model provides the following technical scheme: a cell constant-temperature culture device comprises a base, wherein a box body is arranged on one side of the top of the base, pulleys are arranged at four corners of the bottom of the base, sliding grooves are formed in two sides of the inner wall of the box body, the number of the sliding grooves is two, the number of the sliding grooves in each group is two, and a limiting mechanism is arranged between the two sliding grooves;

the limiting mechanism comprises a fixing plate, the fixing plate is connected with a sliding groove in a sliding mode, a protection plate is arranged at the bottom of the fixing plate, positioning grooves are formed in the fixing plate, the number of the positioning grooves is two, the number of the positioning grooves in each group is multiple, a protection plug is inserted into the positioning grooves and made of rubber materials, a cell culture dish is arranged in the protection plug, a limiting groove is formed in the top of the protection plate, the number of the limiting grooves corresponds to that of the positioning grooves, an arc-shaped protection pad is arranged in the limiting groove, and the limiting groove is matched with the cell culture dish;

the base top opposite side is equipped with the liquid reserve tank, the liquid reserve tank top is equipped with the catheter, the inside water pump that is equipped with of drain pipe, the inside both sides of box all are equipped with the infusion pipeline, the catheter run through the box and extend to the box inner wall, and with infusion pipeline fixed connection, two be equipped with the fluid-discharge tube between the infusion pipeline, the fluid-discharge tube run through the box and extend to box inner wall both sides, and with infusion pipeline fixed connection, the fluid-discharge tube bottom is equipped with the drain hose, drain hose bottom run through the cell culture dish and extend to cell culture dish inner wall, and with cell culture dish threaded connection, drain hose inner wall is equipped with the control valve.

Preferably, the protection plate and the fixing plate are fixedly connected with supporting plates, the number of the supporting plates is two, and the two supporting plates are symmetrically arranged around a central axis of the protection plate.

Preferably, the inside heating wire that is equipped with of box, the quantity of heating wire sets up to two sets of, every group the quantity of heating wire sets up to a plurality ofly, and is a set of the heating wire is located box inner wall top, and opposite side heating wire is located box inner wall bottom, the box inner wall is close to electric heating wire both sides and all is equipped with the heat-conducting plate.

Preferably, a temperature sensor is arranged on one side of the inner wall of the box body, and ultraviolet sterilizing lamps are arranged on the two sides, close to the fixed plate, of the inner wall of the box body.

Preferably, box inner wall one side is equipped with the exhaust fan, exhaust fan outer wall one side is equipped with the air exhauster, the box inner wall is close to that air exhauster one side is articulated to have the flap, exhaust fan outer wall one side is equipped with the deep bead, the deep bead inner wall is equipped with the exhaust pipe, the quantity of exhaust pipe sets up to a plurality ofly, the exhaust pipe inner wall is equipped with the solenoid valve.

Preferably, the box inner wall is kept away from exhaust fan one side and is articulated to have the chamber door, the box top is equipped with control panel, water pump, heating wire, temperature sensor and solenoid valve all with control panel electric connection.

In the technical scheme, the utility model provides the following technical effects and advantages:

1. the limiting tightness of the cell culture dishes is effectively improved through the limiting mechanism, the effect of stable placement is achieved, multiple cells can be cultured simultaneously, mutual influence among the cell culture dishes is prevented, then the water in the liquid storage tank is conveyed to the liquid guide hose through the liquid guide pipe to the liquid guide pipeline and the liquid discharge pipe through the arrangement of the water pump, nutrient solution is dripped into each cell culture dish in sequence, then, external bacteria can be prevented from entering the box body to influence the culture of the cells, the culture quality of the cells is guaranteed, and the culture efficiency of the cells is improved;

2. can heat the box through setting up the heating wire, then can control the cultivation temperature of cell, the heating efficiency is high, make cell culture dish can rapid heating up to required temperature, then circulation and the exchange of the inside hot-air of box can be accelerated to the cooperation exhaust fan, the temperature that makes the box can accord with the living culture environment of cultivateing the cell more, guarantee that cell culture can be stable and normal going on, the air exhauster can carry out the radiating operation fast, sterilamp can disinfect to the box is inside simultaneously, the cell has been guaranteed to cultivate under aseptic condition.

Drawings

In order to more clearly illustrate the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to the drawings.

FIG. 1 is a cross-sectional view of the overall construction of the present invention;

FIG. 2 is a schematic view of the overall structure of the present invention;

FIG. 3 is an enlarged view of the structure of FIG. 1 at A according to the present invention;

FIG. 4 is a schematic view of a connection structure of the limiting mechanism of the present invention;

FIG. 5 is a sectional plan view of the inner structure of the case of the present invention;

FIG. 6 is an enlarged view of the structure of FIG. 5B according to the present invention.

Description of reference numerals:

1. a base; 2. a box body; 3. a pulley; 4. a chute; 5. a fixing plate; 6. a protection plate; 7. a support plate; 8. positioning a groove; 9. a protective plug; 10. a cell culture dish; 11. a limiting groove; 12. an arc-shaped protection pad; 13. a liquid storage tank; 14. a catheter; 15. a water pump; 16. a fluid delivery conduit; 17. a liquid discharge pipe; 18. a liquid guiding hose; 19. a control valve; 20. an electric heating wire; 21. a heat conducting plate; 22. a temperature sensor; 23. an ultraviolet germicidal lamp; 24. an exhaust fan; 25. an exhaust fan; 26. covering the door; 27. a wind deflector; 28. an exhaust duct; 29. an electromagnetic valve; 30. a box door; 31. a control panel.

Detailed Description

In order to make the technical solutions of the present invention better understood, those skilled in the art will now describe the present invention in further detail with reference to the accompanying drawings.

The utility model provides a cell constant-temperature culture device as shown in figures 1-6, which comprises a base 1, wherein one side of the top of the base 1 is provided with a box body 2, four corners of the bottom of the base 1 are respectively provided with a pulley 3, two sides of the inner wall of the box body 2 are respectively provided with a sliding chute 4, the number of the sliding chutes 4 is two, the number of each group of sliding chutes 4 is two, and a limiting mechanism is arranged between the two sliding chutes 4;

the limiting mechanism comprises a fixing plate 5, the fixing plate 5 is in sliding connection with the sliding grooves 4, a protection plate 6 is arranged at the bottom of the fixing plate 5, positioning grooves 8 are formed in the fixing plate 5, the number of the positioning grooves 8 is two, the number of each group of positioning grooves 8 is multiple, a protection plug 9 is inserted in each positioning groove 8, each protection plug 9 is made of rubber materials, a cell culture dish 10 is arranged in each protection plug 9, a limiting groove 11 is formed in the top of the protection plate 6, the number of the limiting grooves 11 corresponds to that of the positioning grooves 8, an arc-shaped protection pad 12 is arranged in each limiting groove 11, and each limiting groove 11 is matched with the cell culture dish 10;

base 1 top opposite side is equipped with liquid reserve tank 13, liquid reserve tank 13 top is equipped with catheter 14, the inside water pump 15 that is equipped with of catheter 14, the inside both sides of 2 boxes all are equipped with infusion pipeline 16, catheter 14 runs through box 2 and extends to 2 inner walls of box, and with infusion pipeline 16 fixed connection, be equipped with fluid-discharge tube 17 between two infusion pipeline 16, fluid-discharge tube 17 runs through box 2 and extends to 2 inner wall both sides of box, and with infusion pipeline 16 fixed connection, fluid-discharge tube 17 bottom is equipped with drain hose 18, drain hose 18 bottom runs through cell culture dish 10 and extends to cell culture dish 10 inner wall, and with cell culture dish 10 threaded connection, drain hose 18 inner wall is equipped with control valve 19.

Further, in the above technical solution, the support plates 7 are fixedly connected between the protection plate 6 and the fixing plate 5, the number of the support plates 7 is two, and the two support plates 7 are symmetrically arranged about the central axis of the protection plate 6.

Further, in the above technical scheme, the inside heating wire 20 that is equipped with of box 2, the quantity of heating wire 20 sets up to two sets of, and the quantity of every group heating wire 20 sets up to a plurality ofly, and a set of heating wire 20 is located 2 inner wall tops of box, and opposite side heating wire 20 is located 2 inner wall bottom of box, and 2 inner walls of box are close to electric heating wire both sides and all are equipped with heat-conducting plate 21.

Furthermore, in the above technical solution, one side of the inner wall of the box body 2 is provided with a temperature sensor 22, and both sides of the inner wall of the box body 2, which are close to the fixing plate 5, are provided with ultraviolet germicidal lamps 23.

Further, in above-mentioned technical scheme, 2 inner walls of box one side are equipped with exhaust fan 24, and 24 outer walls of exhaust fan one side are equipped with air exhauster 25, and 2 inner walls of box are close to that air exhauster 25 one side is articulated to have and cover door 26, and 24 outer walls of exhaust fan one side is equipped with deep bead 27, and deep bead 27 inner wall is equipped with exhaust pipe 28, and the quantity of exhaust pipe 28 sets up to a plurality ofly, and the exhaust pipe 28 inner wall is equipped with solenoid valve 29.

Further, in the above technical solution, a box door 30 is hinged to a side of the inner wall of the box body 2 away from the exhaust fan 24, a control panel 31 is arranged at the top of the box body 2, the water pump 15, the heating wire 20, the temperature sensor 22 and the electromagnetic valve 29 are all electrically connected to the control panel 31, and the type of the temperature sensor 22 is NTC10k 3950.

This practical theory of operation:

referring to the attached drawings 1-6 of the specification, when the utility model is used, firstly, the protective plug 9 is inserted into the positioning groove 8, then the cell culture dish 10 is inserted into the protective plug 9 and the limiting groove 11, the arc-shaped protective pad 12 in the limiting groove 11 protects the bottom of the cell culture dish 10, then the fixed plate 5 is in sliding connection with the sliding groove 4, the fixed plate 5 is pushed into the box body 2, then the liquid guide hose 18 is in threaded connection with the cell culture dish 10, the box door 30 is closed, the limiting tightness of the cell culture dish 10 is effectively enhanced through the protective plug 9 and the arc-shaped protective pad 12 which are arranged in the positioning groove 8 and the limiting groove 11, the effect of stable placement is achieved, the culture of various cells can be simultaneously carried out, the mutual influence between the cell culture dishes 10 is prevented, then the water in the liquid storage tank 13 is conveyed to the liquid conveying pipeline 16 and the liquid discharge pipe 17 through the liquid guide pipe 14 to the liquid guide hose 18 through the water pump 15, then the liquid guide hose 18 is controlled by the control valve 19 to independently and dropwise add nutrient solution to each cell culture dish 10 in sequence, so that external bacteria can be prevented from entering the box body 2 to influence the culture of cells, the culture quality of the cells is ensured, and the culture efficiency of the cells is improved;

referring to the attached drawings 1-6 of the specification, the utility model can heat the box body 2 by arranging the electric heating wire 20, and then can control the culture temperature of cells, the heating efficiency is high, the cell culture dish 10 can be heated to the required temperature quickly, then the air is conveyed to the interior of the box body 2 by the exhaust fan 24 through the exhaust pipe 28 in the wind shield 27, the circulation and the exchange of hot air in the box body 2 can be accelerated by matching with the heat generated by the electric heating wire 20, the exchange and the mixing of cold air and hot air in the box body 2 are accelerated, the temperature of the box body 2 can be more consistent with the living culture environment of the cultured cells, the cell culture can be stably and normally carried out, when the temperature in the box body 2 is higher, the heat dissipation operation can be carried out quickly by opening the exhaust fan 25, the influence is reduced to the lowest, and meanwhile, the ultraviolet sterilizing lamp 23 can sterilize the interior of the box body 2, ensuring the cells to be cultured under the aseptic condition.

While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the utility model. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the utility model.

Claims (6)

1. The utility model provides a cell constant temperature culture apparatus, includes base (1), its characterized in that: a box body (2) is arranged on one side of the top of the base (1), pulleys (3) are arranged at four corners of the bottom of the base (1), sliding grooves (4) are arranged on two sides of the inner wall of the box body (2), the number of the sliding grooves (4) is two, the number of the sliding grooves (4) in each group is two, and a limiting mechanism is arranged between the two sliding grooves (4);

the limiting mechanism comprises a fixing plate (5), the fixing plate (5) is in sliding connection with a sliding groove (4), a protection plate (6) is arranged at the bottom of the fixing plate (5), positioning grooves (8) are formed in the fixing plate (5), the number of the positioning grooves (8) is two, the number of the positioning grooves (8) in each group is multiple, a protection plug (9) is inserted in each positioning groove (8), the protection plug (9) is made of rubber materials, a cell culture dish (10) is arranged in each protection plug (9), a limiting groove (11) is formed in the top of the protection plate (6), the number of the limiting grooves (11) corresponds to that of the positioning grooves (8), an arc-shaped protection pad (12) is arranged in each limiting groove (11), and the limiting grooves (11) are matched with the cell culture dish (10);

the cell culture dish is characterized in that a liquid storage tank (13) is arranged on the other side of the top of the base (1), a liquid guide pipe (14) is arranged on the top of the liquid storage tank (13), a water pump (15) is arranged inside the liquid guide pipe (14), liquid conveying pipelines (16) are arranged on two sides inside the box body (2), the liquid guide pipe (14) penetrates through the box body (2) and extends to the inner wall of the box body (2) and is fixedly connected with the liquid conveying pipelines (16), a liquid discharging pipe (17) is arranged between the two liquid conveying pipelines (16), the liquid discharging pipe (17) penetrates through the box body (2) and extends to two sides of the inner wall of the box body (2) and is fixedly connected with the liquid conveying pipelines (16), a liquid guiding hose (18) is arranged at the bottom of the liquid discharging pipe (17), the bottom of the liquid guiding hose (18) penetrates through the cell culture dish (10) and extends to the inner wall of the cell culture dish (10) and is in threaded connection with the cell culture dish (10), and a control valve (19) is arranged on the inner wall of the liquid guide hose (18).

2. A cell culture apparatus according to claim 1, wherein: the protective plate is characterized in that supporting plates (7) are fixedly connected between the protective plate (6) and the fixing plate (5), the number of the supporting plates (7) is two, and the two supporting plates (7) are symmetrically arranged around the central axis of the protective plate (6).

3. A cell culture apparatus according to claim 1, wherein: the inside heating wire (20) that is equipped with of box (2), the quantity of heating wire (20) sets up to two sets of, every group the quantity of heating wire (20) sets up to a plurality ofly, and is a set of heating wire (20) are located box (2) inner wall top, and opposite side heating wire (20) are located box (2) inner wall bottom, box (2) inner wall all is equipped with heat-conducting plate (21) near the electric heating wire both sides.

4. A cell culture apparatus according to claim 1, wherein: one side of the inner wall of the box body (2) is provided with a temperature sensor (22), and the two sides of the inner wall of the box body (2) close to the fixing plate (5) are provided with ultraviolet germicidal lamps (23).

5. A cell culture apparatus according to claim 1, wherein: box (2) inner wall one side is equipped with exhaust fan (24), exhaust fan (24) outer wall one side is equipped with air exhauster (25), box (2) inner wall is close to that air exhauster (25) one side is articulated to have lid door (26), exhaust fan (24) outer wall one side is equipped with deep bead (27), deep bead (27) inner wall is equipped with exhaust pipe (28), the quantity of exhaust pipe (28) sets up to a plurality ofly, exhaust pipe (28) inner wall is equipped with solenoid valve (29).

6. A cell culture apparatus according to claim 1, wherein: box (2) inner wall is kept away from exhaust fan (24) one side and is articulated to have chamber door (30), box (2) top is equipped with control panel (31), water pump (15), heating wire (20), temperature sensor (22) and solenoid valve (29) all with control panel (31) electric connection.

Images