CN215422473U - Liquid nitrogen quick freezing device for frozen section tissue - Google Patents
Liquid nitrogen quick freezing device for frozen section tissue Download PDFInfo
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- CN215422473U CN215422473U CN202121150072.6U CN202121150072U CN215422473U CN 215422473 U CN215422473 U CN 215422473U CN 202121150072 U CN202121150072 U CN 202121150072U CN 215422473 U CN215422473 U CN 215422473U
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Abstract
The utility model provides a device is deposited to frozen section tissue liquid nitrogen freezes fast, belongs to and freezes deposits technical field of device, including the support body, freeze the storage device who deposits the bucket and save the tissue sample, freeze and deposit the bucket setting on the support body, it can dismantle in the bucket to deposit and be connected with storage device, storage device includes the storage frame, from the top down is equipped with a plurality ofly in proper order and storage frame axis looks vertically baffle in the storage frame, and adjacent baffle constitutes the sample room, it has temperature sensor to freeze bucket internally mounted, the display screen is installed to the support body upper end, temperature sensor will be the temperature transfer to the display screen in the bucket. The tissue sample freezing device is simple in structure, convenient to operate and good in temperature conducting performance, can achieve the purpose of rapidly freezing the tissue sample, provides a buffering effect for the frozen tissue sample, and effectively avoids unnecessary tissue damage caused by cracking of the tissue due to large temperature difference.
Description
Technical Field
The utility model belongs to the technical field of freezing devices, and particularly relates to a liquid nitrogen quick freezing device for frozen section tissues.
Background
In scientific research, frozen sections are an important means for immunohistochemistry and immunofluorescence staining, ice crystals generated in cells of the frozen sections influence the quality of the section tissues, and excessively large ice crystals not only can damage the cell structures of the tissues and influence the observation of the sections, but also influence the reliability of scientific research results. The liquid nitrogen quick freezing can quickly cool the tissue in an ultrashort time, avoid the generation of ice crystals, completely preserve the morphological structure of the tissue, simultaneously protect the antigenicity and the bioactivity of a sample to the maximum extent, and facilitate the subsequent various morphological staining.
In medical experimental research, tissues are generally embedded by OTC and then directly put into liquid nitrogen for rapid cooling, and then the tissue samples are taken out and stored in an ultra-low temperature refrigerator at minus 80 ℃. As a large amount of nitrogen is released in the process of cooling tissues by liquid nitrogen to generate the effect of boiling samples, the OCT glue embedded in the tissues is easy to expand and burst after directly contacting the liquid nitrogen, so that the tissues are broken and the integrity of the tissues is damaged, and the tissue samples frozen by the liquid nitrogen are directly placed into a refrigerator at the temperature of minus 80 ℃, so that the tissues are easy to burst due to large temperature difference.
Disclosure of Invention
In view of the above, the utility model provides a liquid nitrogen rapid freezing device for frozen sliced tissues, which has good temperature conductivity and heat preservation, realizes the purpose of low-temperature rapid freezing, provides a good buffer effect for transferring frozen tissue samples into a-80 refrigerator, and effectively avoids the burst of tissues caused by large temperature difference.
The technical scheme adopted by the utility model for solving the technical problems is as follows:
the utility model provides a frozen section tissue liquid nitrogen freezes deposits device fast, includes the support body, freezes the storage device who deposits bucket and save the tissue sample, freeze and deposit the bucket setting on the support body, it can dismantle in the bucket to freeze and be connected with storage device, storage device includes the storage frame, from the top down is equipped with a plurality of and storage frame axis looks vertically baffle in proper order in the storage frame, and adjacent baffle constitutes the sample room, it has temperature sensor to freeze bucket internally mounted, the display screen is installed to the support body upper end, temperature sensor is with temperature transfer to display screen in the bucket.
Preferably, the inner wall of the freezing storage barrel is provided with a limiting sliding strip parallel to the axis of the freezing storage barrel, the limiting sliding strip is trapezoidal, and the storage device slides into the freezing storage barrel through the limiting sliding strip and is fixed.
Preferably, the storage device further comprises a circular cover plate arranged at the upper end of the storage rack, and the area of the circular cover plate is larger than the cross-sectional area of the storage rack.
Preferably, the freezing barrel comprises a barrel cover and a barrel body, the barrel cover is connected to the frame body in a sliding mode, a clamping groove is formed in the bottom of the barrel cover, and the barrel cover is clamped with the top of the barrel body through the clamping groove.
Preferably, a fastening piece is mounted on the barrel cover and used for locking the barrel cover.
Preferably, a rectangular opening is formed in one end, facing the opening direction of the storage rack, of the partition plate, so that the tissue sample can be taken out conveniently.
Preferably, a space is reserved between the sample chambers, and an activity space is reserved for taking samples.
Preferably, circulation holes are formed in three side walls of the storage frame and are uniformly distributed.
According to the technical scheme, the utility model provides a liquid nitrogen quick freezing device for frozen section tissues, which has the following beneficial effects compared with the prior art: comprises a frame body, a freezing barrel and a storage device for storing frozen section tissues, wherein the freezing barrel is arranged on the frame body, the storage device is detachably connected in the freezing barrel, after the freezing device is placed in liquid nitrogen, the tissue sample is rapidly cooled by transmitting the cold energy of the liquid nitrogen into the freezing barrel, so that the tissue sample is prevented from directly contacting with the liquid nitrogen, a temperature sensor is arranged in the freezing barrel, a display screen is arranged at the upper end of the frame body, the temperature sensor transmits the temperature in the tank to the display screen, when the temperature of the display screen shows that the temperature is-196 ℃, the tissue sample can be taken out of the liquid nitrogen, the storage device is taken out of the cryopreservation barrel and then placed into an ultra-low temperature refrigerator at-80 ℃, the temperature change in the storage device has a buffer period, and the temperature is gradually increased from-196 ℃ to-80 ℃, so that the tissue sample in the device is prevented from being broken due to overlarge temperature difference. The tissue sample freezing device is simple in structure, convenient to operate and good in temperature conducting performance, can achieve the purpose of rapidly freezing the tissue sample, provides a buffering effect for the frozen tissue sample, and effectively avoids unnecessary tissue damage caused by cracking of the tissue due to large temperature difference.
Drawings
FIG. 1 is a schematic structural diagram of a liquid nitrogen rapid cryopreservation device for frozen sliced tissues.
FIG. 2 is a schematic structural diagram of a liquid nitrogen rapid freezing device for frozen tissues after the storage device is taken out.
Fig. 3 is a schematic structural diagram of the memory device.
Fig. 4 is a structural schematic view of the tub cover.
In the figure: the kit comprises a frame body 10, a display screen 11, a freezing barrel 20, a barrel cover 21, a barrel body 22, a fastener 23, a limiting slide bar 24, a temperature sensor 25, a clamping groove 26, a storage device 30, a storage frame 31, a circular cover plate 32, a partition plate 33, a sample chamber 34, a circulation hole 35 and a tissue sample 40.
Detailed Description
The technical solutions and effects of the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings of the present invention.
Referring to fig. 1 to 4, the utility model provides a liquid nitrogen rapid freezing device for frozen section tissues, which comprises a frame body 10, a freezing barrel 20 and a storage device 30 for storing tissue samples 40, wherein the freezing barrel 20 is arranged on the frame body 10, the storage device 30 is detachably connected in the freezing barrel 20, the storage device 30 comprises a storage rack 31, a plurality of partition plates 33 which are vertical to the axis of the storage rack 31 are sequentially arranged in the storage rack 31 from top to bottom, adjacent partition plates 33 form a sample chamber 34, a temperature sensor 25 is arranged in the freezing barrel 20, a display screen 11 is arranged at the upper end of the frame body, and the temperature sensor 25 transmits the temperature in the barrel to the display screen 11.
In this embodiment, the number of the sample chambers 34 formed in the storage rack 31 is 8, and the size of the sample chambers 34 can be set according to the size of the embedding cassette for embedding the tissue.
Further, the inner wall of the freezing barrel 20 is provided with a limiting sliding strip 24 parallel to the axis of the freezing barrel 20, the limiting sliding strip 24 is trapezoidal, and the storage device 30 slides into the freezing barrel 20 through the limiting sliding strip 24 and is fixed.
Further, the storage device 30 further comprises a circular cover plate 32 mounted on the upper end of the storage rack 31, and the area of the circular cover plate 32 is larger than the cross-sectional area of the storage rack 31. When the storage rack is integrally placed in the freezing barrel, the round cover plate is clamped on the limiting sliding strip to indicate that the storage device is fixed in the freezing barrel.
Further, the freezing barrel 20 comprises a barrel cover 21 and a barrel body 22, the barrel cover 21 is slidably connected to the frame body 10, a clamping groove 26 is formed in the bottom of the barrel cover 21, and the barrel cover 21 is clamped with the top of the barrel body 22 through the clamping groove 26.
Further, a fastening member 23 is further mounted on the barrel cover 21, and the fastening member 23 is used for locking the barrel cover 21.
Furthermore, a rectangular opening is formed at one end of the partition plate 33 facing the opening direction of the storage rack 31, so that the tissue sample can be taken out conveniently. The tissue sample is embedded in the embedding box through the OTC, and the size of embedding box is slightly less than the size of sample room, and the rectangle mouth of seting up on the baffle makes when taking out the tissue sample more convenient.
Furthermore, a space is left between the sample chambers 34 to reserve an active space for taking samples.
Further, circulation holes 35 are formed in three side walls of the storage rack 31, and the circulation holes 35 are uniformly distributed, so that the cold energy of the liquid nitrogen is fully transmitted to the tissue sample 40.
When the device is used, the storage device 30 is taken out of the freezing barrel 20 and placed on a laboratory table, the pre-embedded tissue samples 40 are placed into the sample chamber 34 one by one, and an interval is reserved between every two adjacent tissue samples 40 so as to be taken later; then sliding the storage device 20 into the freezing barrel 20 along the limiting sliding strip 24 until the circular cover plate 32 is clamped on the limiting sliding strip 24, indicating that the storage device 30 is fixed in the freezing barrel 20, sliding the barrel cover 21 downwards from the frame body 10 until the barrel cover 21 is clamped with the barrel body 22, and screwing the fastener 23 to enable the freezing barrel 20 to be in a sealed state to prevent liquid nitrogen from entering the freezing barrel 20; then the whole cryopreservation device is placed into liquid nitrogen for cryopreservation of the tissue sample 40 during working, the temperature sensor 25 arranged inside the barrel body detects the temperature inside the barrel and transmits temperature information to the display screen 11 at the upper end of the frame body 10, a worker can know the cryopreservation temperature of the tissue sample 40 according to the display screen 11, when the temperature value reaches-196 ℃, the cryopreservation device 30 is taken out of the liquid nitrogen, the storage device 30 can be directly placed into a-80 ℃ ultra-low temperature refrigerator after being taken out of the cryopreservation barrel 20, the tissue is respectively placed into self-sealing sample bags for classified storage after being buffered for half an hour, and further burst caused by large temperature difference of the tissue is avoided.
While the utility model has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the utility model.
Claims (8)
1. The utility model provides a frozen section tissue liquid nitrogen freezes deposits device fast which characterized in that: including the support body, freeze the storage device who deposits bucket and save the tissue sample, freeze and deposit the bucket setting on the support body, it can dismantle in the bucket to be connected with to freeze to deposit storage device, storage device includes the storage frame, from the top down is equipped with a plurality ofly in proper order and storage frame axis looks vertically baffle in the storage frame, and adjacent baffle constitutes the sample room, freeze and deposit bucket internally mounted and have temperature sensor, the display screen is installed to the support body upper end, temperature sensor will be the temperature transfer to the display screen in the bucket.
2. The liquid nitrogen fast freezing device for the frozen section tissue according to claim 1, characterized in that: the inner wall of the freezing barrel is provided with a limiting sliding strip parallel to the axis of the freezing barrel, the limiting sliding strip is trapezoidal, and the storage device slides into the freezing barrel through the limiting sliding strip and is fixed.
3. The liquid nitrogen fast freezing device for the frozen section tissue according to claim 1, characterized in that: the storage device also comprises a circular cover plate arranged at the upper end of the storage rack, and the area of the circular cover plate is larger than the cross section area of the storage rack.
4. The liquid nitrogen fast freezing device for the frozen section tissue according to claim 1, characterized in that: the freezing barrel comprises a barrel cover and a barrel body, the barrel cover is connected to the frame body in a sliding mode, a clamping groove is formed in the bottom of the barrel cover, and the barrel cover is connected with the top of the barrel body in a clamping mode through the clamping groove.
5. The liquid nitrogen fast freezing device for the frozen section tissue as claimed in claim 4, wherein: and a fastening piece is also arranged on the barrel cover and used for locking the barrel cover.
6. The liquid nitrogen fast freezing device for the frozen section tissue according to claim 1, characterized in that: the baffle has seted up the rectangle mouth towards the one end of storage frame opening direction, conveniently takes out and organizes the sample.
7. The liquid nitrogen fast freezing device for the frozen section tissue according to claim 1, characterized in that: and a space is reserved between the sample chambers to reserve an activity space for taking samples.
8. The liquid nitrogen fast freezing device for the frozen section tissue according to claim 1, characterized in that: circulation holes are formed in three side walls of the storage frame and are evenly distributed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121150072.6U CN215422473U (en) | 2021-05-26 | 2021-05-26 | Liquid nitrogen quick freezing device for frozen section tissue |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121150072.6U CN215422473U (en) | 2021-05-26 | 2021-05-26 | Liquid nitrogen quick freezing device for frozen section tissue |
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| CN215422473U true CN215422473U (en) | 2022-01-07 |
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| CN202121150072.6U Active CN215422473U (en) | 2021-05-26 | 2021-05-26 | Liquid nitrogen quick freezing device for frozen section tissue |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115226707A (en) * | 2022-09-02 | 2022-10-25 | 赣南创新与转化医学研究院 | Method for freezing and slicing tissue stored at low temperature and application |
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2021
- 2021-05-26 CN CN202121150072.6U patent/CN215422473U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115226707A (en) * | 2022-09-02 | 2022-10-25 | 赣南创新与转化医学研究院 | Method for freezing and slicing tissue stored at low temperature and application |
| CN115226707B (en) * | 2022-09-02 | 2024-02-02 | 赣南创新与转化医学研究院 | Method for frozen section of low-temperature frozen tissue and application thereof |
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