CN215404303U

CN215404303U - Animal cell suspension culture device

Info

Publication number: CN215404303U
Application number: CN202121688247.9U
Authority: CN
Inventors: 朱世福; 刘晓伟
Original assignee: Guangzhou Haoxin Cleaning Engineering Technology Co ltd
Current assignee: Guangzhou Haoxin Cleaning Engineering Technology Co ltd
Priority date: 2021-07-23
Filing date: 2021-07-23
Publication date: 2022-01-04
Anticipated expiration: 2031-07-23

Abstract

The utility model discloses an animal cell suspension culture device, which comprises: the supporting device comprises a base, a supporting cylinder and a supporting frame, wherein the supporting cylinder is vertically arranged on the base, a cavity is arranged in the base, and the interior of the supporting cylinder is communicated with the cavity; a plurality of convex blocks are arranged on the upper end surface of the supporting cylinder along the circumferential direction, and inclined guide surfaces are arranged on the convex blocks according to the two ends of the supporting cylinder in the circumferential direction; the rotating shaft is vertically arranged at the center of the bottom of the rotating disc, is sleeved in the supporting legs, and the end part of the rotating shaft enters the cavity; the bottom of the rotary table is provided with a vibration ring used for abutting against the support cylinder, the vibration ring is provided with a plurality of grooves along the circumferential direction on the end surface of the support cylinder, and the grooves correspond to the lugs one to one; a plurality of culture dishes are arranged on the rotary disc; and the motor is arranged in the cavity and is used for driving the rotating shaft to rotate. The culture dish on the rotary plate can be simultaneously rotated and vibrated, so that the animal cells are more dispersed in the culture solution in the culture dish.

Description

Animal cell suspension culture device

Technical Field

The utility model relates to the technical field of animal cell culture, in particular to an animal cell suspension culture device.

Background

Suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid culture medium which is constantly stirred or shaken, and is a culture mode of anchorage-independent cells. Certain anchorage-dependent cells can also be cultured using this method, after adaptation and selection. The method is a culture method which ensures that cells are always in dispersed suspension in a culture solution by shaking or rotating a device. However, the existing suspension culture device generally has only one of the functions of rotation, oscillation or stirring, so that the effect of dispersing cells in the culture solution is not good enough and needs to be improved.

SUMMERY OF THE UTILITY MODEL

In view of the above, the present invention provides an animal cell suspension culture apparatus, which can solve, at least to some extent, the problem that the prior suspension culture apparatus has insufficient effect of dispersing cells in a culture solution.

The technical scheme of the utility model is realized as follows:

an animal cell suspension culture apparatus comprising:

the supporting device comprises a base, a supporting cylinder and a supporting frame, wherein the supporting cylinder is vertically arranged on the base, a cavity is arranged in the base, and the interior of the supporting cylinder is communicated with the cavity; a plurality of convex blocks are arranged on the upper end surface of the supporting cylinder along the circumferential direction, and inclined guide surfaces are arranged on the convex blocks according to the two ends of the supporting cylinder in the circumferential direction;

a rotating shaft is vertically arranged at the center of the bottom of the rotating disc, the rotating shaft is sleeved in the supporting cylinder, and the end part of the rotating shaft enters the cavity; the bottom of the rotary table is provided with a vibration ring used for abutting against the support cylinder, the vibration ring is provided with a plurality of grooves along the circumferential direction on the end surface of the support cylinder, and the grooves correspond to the lugs one to one; a plurality of culture dishes are arranged on the rotary disc;

and the motor is arranged in the cavity and is used for driving the rotating shaft to rotate.

As a further alternative of the animal cell suspension culture device, a guide hole with a polygonal cross section is concavely arranged at the bottom end of the rotating shaft; a rotatable driver is arranged in the cavity, the driver and the rotating shaft are coaxially arranged, the driver comprises a gear part and a guide pillar which is coaxially arranged above the gear part, and the guide pillar is movably sleeved in the guide hole; the motor is in transmission connection with the gear part.

As a further alternative of the animal cell suspension culture device, the cross section of the guide hole is in a regular hexagon, and the shape of the guide pillar corresponds to the guide hole.

As a further alternative of the animal cell suspension culture device, the end part of the rotating shaft enters the cavity and then is connected with a limiting disc, a spring sleeved on the rotating shaft is arranged in the cavity, one end of the spring is abutted against the inner wall of the cavity, and the other end of the spring is abutted against the limiting disc.

As a further alternative of the animal cell suspension culture device, the number of the projections and the grooves is two.

As a further alternative of the animal cell suspension culture device, a plurality of fixing holes are formed in the centrifugal position on the rotating disc along the circumferential direction, and the culture dish is erected in the fixing holes.

The utility model has the following beneficial effects: the rotating shaft is driven to rotate by a motor, so that the rotating disc is driven to rotate, and the culture dish on the rotating disc is subjected to a rotating effect; the rotary disc moves up and down in the rotating process by utilizing the plurality of lugs on the supporting cylinder, so that the culture dish on the rotary disc is subjected to a vibration effect; so that the animal cells are more dispersed in the culture solution in the culture dish.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention.

FIG. 1 is a schematic view of the structure of an animal cell suspension culture apparatus according to the present invention;

FIG. 2 is an exploded view of the animal cell suspension culture apparatus of the present invention;

FIG. 3 is a schematic cross-sectional view of an animal cell suspension culture apparatus of the present invention;

FIG. 4 is a schematic structural view of the support cylinder;

FIG. 5 is a schematic structural view of the vibration ring;

fig. 6 is a schematic view of the connection between the driver and the spindle.

In the figure: 1. a base; 11. a cavity; 2. a turntable; 21. a fixing hole; 3. a support cylinder; 31. a bump; 311. an inclined guide surface; 4. a rotating shaft; 41. a guide hole; 42. a limiting disc; 5. a motor; 6. a driver; 61. a gear portion; 62. a guide post; 7. a spring; 8. a vibrating ring; 81. a groove; 9. a culture dish.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. Based on the embodiments of the utility model.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "above," and "over" a second feature may mean that the first feature is directly above or obliquely above the second feature, or that only the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

Referring to fig. 1-6, there is shown an animal cell suspension culture apparatus comprising:

the device comprises a base 1, wherein a supporting cylinder 3 is vertically arranged on the base 1, a cavity 11 is arranged in the base 1, and the inside of the supporting cylinder 3 is communicated with the cavity 11; a plurality of convex blocks 31 are arranged on the upper end surface of the supporting cylinder 3 along the circumferential direction, and inclined guide surfaces 311 are arranged on the convex blocks 31 according to the two ends of the supporting cylinder 3 in the circumferential direction;

a rotating shaft 4 is vertically arranged at the center of the bottom of the rotating disc 2, the rotating shaft 4 is sleeved in the supporting cylinder, and the end part of the rotating shaft 4 enters the cavity 11; the bottom of the turntable 2 is provided with a vibration ring 8 used for abutting against the support cylinder 3, the vibration ring 8 is provided with a plurality of grooves 81 along the circumferential direction on the end surface of the support cylinder 3, and the grooves 81 are in one-to-one correspondence with the lugs 31; a plurality of culture dishes 9 are arranged on the turntable 2;

and the motor 5 is arranged in the cavity 11 and is used for driving the rotating shaft 4 to rotate.

In brief, the motor 5 is used for driving the rotating shaft 4 to rotate, so as to drive the rotating disc 2 to rotate, and the culture dish 9 on the rotating disc 2 is subjected to a rotating effect; meanwhile, when the rotating shaft 4 rotates, the vibrating ring 8 rotates synchronously, and the supporting cylinder 3 is in a fixed state, so that in the rotating process of the vibrating ring 8, two conditions that the lug 31 is clamped into the groove 81 and the lug 31 is separated from the groove 81 exist, and in the two conditions, when the lug 31 is clamped into the groove 81, the turntable 2 is at a lower height; when the projection 31 is out of the groove 81, the rotary disc 2 is at a higher height; in this way, the turntable 2 moves up and down during rotation, thereby creating a vibratory effect. Wherein the inclined guide surface 311 on the protrusion 31 can facilitate the protrusion 31 to disengage from the groove 81.

In some specific embodiments, since the rotating shaft 4 also moves up and down during the rotation process, in order to facilitate the motor 5 to drive the rotating shaft 4 to rotate, referring to fig. 2, 3 and 6, a guide hole 41 with a polygonal cross section is recessed at the bottom end of the rotating shaft 4; a rotatable driver 6 is arranged in the cavity 11, the driver 6 and the rotating shaft 4 are coaxially arranged, the driver 6 comprises a gear part 61 and a guide post 62 coaxially arranged above the gear part 61, and the guide post 62 is movably sleeved in the guide hole 41; the motor 5 is in transmission connection with the gear part 61. Preferably, the cross section of the guide hole 41 is a regular hexagon, and the guide post 62 is shaped to correspond to the guide hole 41. In other words, the motor 5 drives the driver 6 to rotate, and the driver 6 drives the rotating shaft 4 to rotate through the guide post 62, and when the rotating shaft 4 moves up and down during rotation, the guide hole 41 and the guide post 62 move relatively in the radial direction; in this way, the positions of the driver 6 and the motor 5 are unchanged, ensuring a stable driving process.

In the above scheme, in order to ensure that the protruding block 31 can be clamped into the groove 81, referring to fig. 2 and fig. 3, the end portion of the rotating shaft 4 enters the cavity 11 and then is connected with the limiting disc 42, a spring 7 sleeved on the rotating shaft 4 is arranged in the cavity 11, one end of the spring 7 abuts against the inner wall of the cavity 11, and the other end of the spring 7 abuts against the limiting disc 42. Therefore, the spring 7 keeps the rotating shaft 4 moving downwards, and the situation that the bump 31 does not enter the groove 81 under the driving of the centrifugal force of the limiting disc 42 in the rotating process is avoided. Wherein, the number of the convex blocks 31 and the concave grooves 81 may be two.

In the above scheme, in order to facilitate the fixing of the culture dish 9 on the turntable 2, referring to fig. 2, a plurality of fixing holes 21 are formed in the centrifugal position on the turntable 2 along the circumferential direction, and the culture dish 9 is erected in the fixing holes 21. Thus, the culture dish 9 is stably placed on the rotary table 2 and cannot topple over even if rotating and vibrating simultaneously; in addition, the device is convenient to take and place.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. An animal cell suspension culture device, comprising:

2. The animal cell suspension culture device of claim 1, wherein the bottom end of the rotating shaft is concavely provided with a guide hole with a polygonal cross section; a rotatable driver is arranged in the cavity, the driver and the rotating shaft are coaxially arranged, the driver comprises a gear part and a guide pillar which is coaxially arranged above the gear part, and the guide pillar is movably sleeved in the guide hole; the motor is in transmission connection with the gear part.

3. The animal cell suspension culture device of claim 2, wherein the cross section of the guide hole is regular hexagon, and the shape of the guide pillar corresponds to the guide hole.

4. The animal cell suspension culture device of claim 2, wherein a limiting disc is connected to the end of the rotating shaft after entering the cavity, a spring sleeved on the rotating shaft is arranged in the cavity, one end of the spring abuts against the inner wall of the cavity, and the other end of the spring abuts against the limiting disc.

5. The animal cell suspension culture apparatus of claim 1, wherein the number of the projections and the grooves is two.

6. The animal cell suspension culture device according to claim 1, wherein a plurality of fixing holes are formed in the centrifugal position of the turntable in the circumferential direction, and the culture dish is erected in the fixing holes.