CN215339479U

CN215339479U - Light-activated chemiluminescence detection system

Info

Publication number: CN215339479U
Application number: CN202121736583.6U
Authority: CN
Inventors: 温强; 黄毅; 金成�
Original assignee: Guangzhou Weiguang Technology Co ltd
Current assignee: Guangzhou Weiguang Technology Co ltd
Priority date: 2021-07-28
Filing date: 2021-07-28
Publication date: 2021-12-28
Anticipated expiration: 2031-07-28

Abstract

The utility model provides a light-activated chemiluminescence detection system, and relates to the technical field of light-activated chemiluminescence; the laser light path and the detection light path are respectively and independently arranged above the mobile platform; the mobile platform is provided with a plurality of sample holes for placing samples, and any sample hole on the mobile platform can move to and fro below the laser light path and below the detection light path. The laser light path and the detection light path in the light-activated chemiluminescence detection system are independently arranged, so that the influence of laser on a photomultiplier of a detector in-situ detection is completely avoided, a large number of optical lenses are not needed, the structure is simple, and the manufacturing cost is lower.

Description

Light-activated chemiluminescence detection system

Technical Field

The utility model relates to the technical field of light-activated chemiluminescence, in particular to a light-activated chemiluminescence detection system.

Background

The light-activated chemiluminescence technology uses a homogeneous system chemiluminescence detection technology, and an antibody participating in immunoreaction is coated with a photosensitive bead containing phthalocyanine; the other antibody is coated with luminescent beads, and contains a dimethylthiophene derivative and Eu chelate. In the presence of a target antigen, a sandwich immune complex can be formed, the target antigen can enable two photosensitive beads and luminescent beads which are marked on the antibodies to be tightly connected together, and a luminol oxidation chemiluminescence process can be completed under 680nm excitation light.

The light-activated chemiluminescence analyzer mainly comprises a light-activated system and a chemiluminescence detection system. The optical laser system generally adopts a 680nm laser, and adopts proper optical power to carry out optical laser irradiation on the sample; the chemiluminescence detection system mainly adopts a high-sensitivity photomultiplier as a detector, a sample emits chemiluminescence between 500-620nm after being irradiated by 680nm laser, the chemiluminescence is collected by the photomultiplier to output a weak current signal, the current signal is amplified and converted into a pulse signal to be output, and the pulse signal is read and output by computer upper computer software.

In order to realize the light excitation and luminescence detection of a sample at the same time, the prior art adopts in-situ detection, which is the most conventional and universal detection method, but the in-situ detection has the following defects:

1. the structure is very complicated, a large number of optical lenses such as lenses, dichroic mirrors and reflecting mirrors are adopted, the requirements on angles and distances among the optical lenses are very strict, and the requirement on machining precision is very high;

2. the manufacturing cost is high, the optical lens is very expensive, and the machining cost of the matched device is also very high;

3. although a short-wave-pass dichroic mirror is used, 685nm high-intensity laser can still not completely block laser energy even if 99.5% of energy can be reflected, the rest 0.5% of energy passes through a reflecting mirror after penetrating through the reflecting mirror, and although the negative high voltage of the photomultiplier is closed when the laser irradiates a sample, the photocathode material of the photomultiplier still absorbs the laser energy, and when the negative high voltage power supply is opened, the dark current rises, and experimental tests show that certain laser signal interference still exists.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a light-activated chemiluminescence detection system, which can completely avoid the influence of laser on a photomultiplier of a detector in-situ detection, does not need to use a large number of optical lenses, and has simple structure and lower manufacturing cost.

In order to achieve the purpose, the utility model provides the following technical scheme:

the utility model provides a light-activated chemiluminescence detection system which comprises a mobile platform, a laser light path and a detection light path, wherein the mobile platform is provided with a light source;

the laser light path and the detection light path are respectively and independently arranged above the mobile platform;

the mobile platform is provided with a plurality of sample holes for placing samples, and any sample hole on the mobile platform can move to and fro below the laser light path and below the detection light path.

Further, the moving platform comprises an X-direction slide rail, a Y-direction moving device and a porous plate assembly;

the Y-direction moving device is arranged on the X-direction sliding rail in a sliding manner along the X direction;

the multi-well plate component is slidably mounted on the Y-direction moving device along the Y direction, and is provided with a plurality of sample holes.

Further, the Y-direction moving device comprises a sliding block and a Y-direction sliding rail connected with the sliding block;

the sliding block is slidably mounted on the X-direction sliding rail;

the porous plate component is arranged on the Y-direction slide rail in a sliding manner along the Y direction.

Further, the porous plate assembly comprises a porous plate body and a supporting seat;

the porous plate body is connected with the top surface of the supporting seat and is provided with a plurality of sample holes;

the bottom surface of the supporting seat is slidably mounted on the Y-direction sliding rail along the Y direction.

Furthermore, the laser light path comprises a laser light source, an optical fiber and a collimator, and two ends of the optical fiber are respectively connected with the laser light source and the collimator.

Furthermore, the detection light path comprises a quartz optical window, an optical filter, a photomultiplier tube and a photomultiplier tube sleeve, wherein the quartz optical window and the optical filter are installed on the photomultiplier tube, and the photomultiplier tube sleeve is sleeved outside the photomultiplier tube.

Furthermore, the detection light path further comprises a heat dissipation structure, and the heat dissipation structure penetrates through the photomultiplier tube sleeve and is connected with the photomultiplier tube.

Further, the laser light path detection device comprises a shell, the mobile platform is installed in the shell, the laser light path and the detection light path are installed at the top of the shell, a first light-transmitting opening is formed in the position, corresponding to the laser light path, of the top of the shell, and a second light-transmitting opening is formed in the position, corresponding to the detection light path, of the top of the shell.

Further, the housing comprises a shell and a taking and placing door;

the top of the shell is provided with a taking and placing opening, and the first light transmission opening and the second light transmission opening are formed in the shell;

the taking and placing door is arranged at the taking and placing opening in a sliding mode.

Furthermore, a sliding rail is arranged at the position of the taking and placing opening of the shell, and the taking and placing door is matched with the sliding rail.

The light-activated chemiluminescence detection system provided by the utility model can produce the following beneficial effects:

when the light-activated chemiluminescence detection system works, firstly, the detection sample hole is aligned to the laser light path by controlling the moving platform, the laser light path irradiates a sample, then, the sample hole to be detected is rapidly aligned to the detection light path by moving the moving platform, and the detection light path collects chemiluminescence of the sample. Compared with the prior art, the laser light path and the detection light path in the light-activated chemiluminescence detection system are independently arranged, so that the influence of laser on a photomultiplier of a detector in-situ detection is completely avoided, a large number of optical lenses are not needed, the structure is simple, and the manufacturing cost is lower.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic three-dimensional structure diagram of a light-activated chemiluminescence detection system provided by an embodiment of the utility model;

FIG. 2 is a top view of a light activated chemiluminescent detection system provided by the present embodiment of the utility model;

FIG. 3 is a schematic three-dimensional structure of a multi-well plate assembly according to an embodiment of the present invention;

FIG. 4 is a schematic three-dimensional structure diagram of another light-activated chemiluminescent detection system provided by the present invention;

FIG. 5 is a schematic diagram of the internal structure of another light-activated chemiluminescent detection system provided by the embodiment of the present invention;

FIG. 6 is a top view of a housing provided in accordance with an embodiment of the present invention;

fig. 7 is a schematic three-dimensional structure diagram of a pick-and-place door according to an embodiment of the present invention.

Icon: 1-moving a platform; 11-X direction sliding rails; a 12-Y directional moving device; 121-a slider; a 122-Y-direction slide rail; 13-a perforated plate assembly; 131-a porous plate body; 1311-sample well; 132-a support base; 2-laser light path; 21-an optical fiber; 22-a collimator; 3-detection of the optical path; 31-a photomultiplier tube; 32-photomultiplier tube housing; 33-a heat dissipation structure; 4-a housing; 41-a housing; 411-a first light transmission opening; 412-a second light transmission opening; 413-track; 42-a pick-and-place door; 421-a chute; 43-a separator; 5-support plate.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The following detailed description of embodiments of the utility model refers to the accompanying drawings. It should be understood that the detailed description and specific examples, while indicating the present invention, are given by way of illustration and explanation only, not limitation.

The present embodiment provides a light-activated chemiluminescence detection system, as shown in fig. 1 and 2, including a mobile platform 1, a laser light path 2, and a detection light path 3; the laser light path 2 and the detection light path 3 are respectively and independently arranged above the mobile platform 1; the mobile platform 1 is provided with a plurality of sample holes 1311 for placing samples, and any one sample hole 1311 on the mobile platform 1 can move to and fro below the laser light path 2 and below the detection light path 3.

The principle of the photoluminescence is that firstly the sample is irradiated by laser, then the sample chemiluminescence is collected, therefore, the sample hole 1311 is firstly aligned to the laser light path 2 by the mobile platform 1, and the sample hole 1311 is quickly aligned to the detection light path 3 by the mobile platform 1 after the laser irradiation is finished, so that the detection purpose can be completely realized.

The core concept of the light-activated chemiluminescence detection system provided by this embodiment is to independently set the laser light path 2 and the detection light path 3, and then realize the first laser irradiation and the second luminescence detection of the sample through the mobile platform 1. Therefore, the moving direction of the moving stage 1 is not limited, and the moving stage 1 may be moved in any one or more directions X, Y, Z in which any one of the sample wells 1311 can be moved to and fro below the laser beam path 2 and below the detection beam path 3.

The structure of the mobile platform 1 is explained in detail below:

in some embodiments, as shown in fig. 1 to 3, in order to align each sample well with the laser light path 2 and the detection light path 3, the moving platform 1 includes an X-directional slide rail 11, a Y-directional moving device 12, and a multi-well plate assembly 13; the Y-direction moving device 12 is arranged on the X-direction slide rail 11 in a sliding manner along the X direction; the multi-well plate assembly 13 is slidably mounted to the Y-direction moving device 12 in the Y direction, and the multi-well plate assembly 13 has a plurality of sample wells 1311.

Specifically, in use, the Y-direction moving device 12 may slide relative to the X-direction slide rail 11 to adjust the X-direction position of the sample well 1311, and the multi-well plate assembly 13 may slide relative to the Y-direction moving device 12 to adjust the Y-direction position of the sample well 1311.

The moving platform 1 can realize the movement of the sample holes 1311 in the horizontal direction, so as shown in fig. 3, the sample holes 1311 in the multi-hole plate assembly 13 can be distributed on a plane, and compared with the moving platform 1 which can only move along a straight line, more samples can be detected at the same time, compared with the moving platform 1 which can move along X, Y, Z three directions, the cost is lower, and only the distance between the multi-hole plate assembly 13 and the laser light path 2 and the detection light path 3 needs to be adjusted along the vertical direction before assembly.

In some embodiments, as shown in fig. 1, the Y-direction moving device 12 includes a slider 121 and a Y-direction sliding rail 122 connected to the slider 121; the slider 121 is slidably mounted on the X-direction slide rail 11; the perforated plate assembly 13 is slidably mounted on the Y-direction slide rail 122 in the Y-direction.

The Y-direction moving device 12 has a simple structure, and the slider 121 can support the Y-direction slide rail 122 on one hand and slide relative to the X-direction slide rail 11 on the other hand, so as to drive the Y-direction slide rail 122 to move along the X-direction.

In some embodiments, as shown in fig. 3, the perforated plate assembly 13 includes a perforated plate body 131 and a support seat 132; the multi-well plate body 131 is connected to the top surface of the support base 132, and the multi-well plate body 131 has a plurality of sample wells 1311; the bottom surface of the support base 132 is slidably mounted on the Y-rail 122 along the Y-direction.

Specifically, the top surface of supporting seat 132 is provided with the jack catch, and porous plate body 131 can be through the jack catch joint on supporting seat 132, so when needs are to porous plate body 131 application of sample, can take off porous plate body 131 earlier, and the application of sample finishes the back, can be with porous plate body 131 joint on supporting seat 132, and the application of sample is more convenient.

The clamping jaws can adopt spring pieces, elastic blocks and other structures.

The sliding of the slider 121 with respect to the X-direction slide rail 11 and the sliding of the support 132 with respect to the Y-direction slide rail 122 may be achieved by a linear motor, or may be achieved by a pneumatic cylinder, a hydraulic cylinder, or the like.

The structure of the laser beam path 2 will be specifically described below:

in some embodiments, as shown in fig. 1, the laser path 2 includes a laser light source (not shown), an optical fiber 21 and a collimator 22, two ends of the optical fiber 21 are respectively connected to the laser light source and the collimator 22, and the collimator 22 can convert divergent light transmitted from the optical fiber 21 into parallel light through a lens disposed in front of the collimator and direct the parallel light toward the sample hole 1311.

The position of the laser light path 2 may be fixed by a bracket or may be fixed by being mounted on a certain housing.

The structure of the detection optical path 3 will be specifically described below:

in some embodiments, as shown in fig. 1 and 2, the detection optical path 3 includes a quartz optical window, an optical filter, a photomultiplier tube 31, and a photomultiplier tube housing 32, the quartz optical window and the optical filter are mounted on the photomultiplier tube 31, and the photomultiplier tube housing 32 is mounted outside the photomultiplier tube 31.

In at least one embodiment, the detection optical path 3 is located at the side of the laser optical path 2, and the light entrance window of the photomultiplier tube 31 and the light emitting end of the collimator 22 are located on the same plane, so that the detection optical path 3 can accurately collect chemiluminescence of the sample.

In some embodiments, as shown in fig. 1 and 2, the detection optical path 3 further includes a heat dissipation structure 33, and the heat dissipation structure 33 is connected to the photomultiplier tube 31 through the photomultiplier tube housing 32.

The heat dissipation structure 33 can increase the contact area between the photomultiplier tube 31 and the air, thereby increasing the heat dissipation of the photomultiplier tube 31.

Specifically, the heat dissipation structure 33 includes a bracket and a plurality of rows of heat dissipation columns connected to the bracket, the bracket passes through the photomultiplier tube sleeve 32 and is connected to the photomultiplier tube 31, and the bracket transfers heat of the photomultiplier tube 31 to the plurality of rows of heat dissipation columns, and the heat is dissipated through the plurality of rows of heat dissipation columns.

A preferred embodiment of the optically activated chemiluminescent detection system is described in detail below:

in some embodiments, as shown in fig. 4, the light-activated chemiluminescent detection system further comprises a housing 4, the mobile platform 1 is mounted within the housing 4, and the housing 4 can provide a relatively dark detection chamber; laser light path 2 and detection light path 3 install in the top of shell 4, and the position that the top of shell 4 corresponds laser light path 2 has first light transmission opening 411, and the laser that laser light path 2 sent can shine to sample hole 1311 from first light transmission opening 411, and the position that the top of shell 4 corresponds detection light path 3 has second light transmission opening 412, and the luminous of sample can get into detection light path 3 from second light transmission opening 412.

Because the fluorescence or chemiluminescence emitted by the sample after laser excitation is weak light, the sample can be protected from light by the arrangement of the shell 4, and the accuracy of the detection result is ensured.

Specifically, as shown in fig. 5, the housing 4 has a double-layer structure, that is, a partition 43 is disposed in the housing 4, a through slot is formed in the partition 43, the X-direction slide rail 11 is disposed at the bottom layer of the housing 4, the slider 121 penetrates through the through slot, the Y-direction slide rail 122 is disposed at the top layer of the housing 4, a support plate 5 is connected to the side of the Y-direction slide rail 122, and the support plate 5 is supported on the partition 43.

The Y-direction slide rail 122 is large in size in the longitudinal direction thereof, and the connection surface with the X-direction slide rail 11 via the slider 121 is small, so that the Y-direction slide rail is likely to be inclined during operation. The arrangement of the supporting plate 5 and the partition plate 43 can play a certain supporting role on the Y-direction sliding rail 122, so as to ensure the operation stability of the Y-direction sliding rail 122.

In some embodiments, as shown in fig. 6, to facilitate access to the porous plate body 131, the housing 4 includes a housing 41 and an access door 42; the top of the housing 41 has a pick-and-place opening, and the first light-transmitting opening 411 and the second light-transmitting opening 412 are opened in the housing 41; the pick-and-place door 42 is slidably disposed at the pick-and-place opening.

The detection personnel can install the porous plate body 131 on the supporting seat 132 through the taking and placing opening, and after the installation is finished, the taking and placing door 42 slides to cover the taking and placing opening.

In some embodiments, as shown in fig. 6 and 7, the access opening of the housing 41 has a rail 413, and the access door 42 has a sliding slot 421 matching with the rail 413, so as to ensure the sliding stability of the access door 42.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the utility model has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A light-activated chemiluminescence detection system is characterized by comprising a mobile platform (1), a laser light path (2) and a detection light path (3);

the laser light path (2) and the detection light path (3) are respectively and independently arranged above the mobile platform (1);

the mobile platform (1) is provided with a plurality of sample holes (1311) for placing samples, and any sample hole (1311) on the mobile platform (1) can move to and fro below the laser light path (2) and below the detection light path (3).

2. The light-activated chemiluminescent detection system of claim 1 wherein the moving platform (1) comprises an X-direction slide rail (11), a Y-direction moving device (12) and a multi-well plate assembly (13);

the Y-direction moving device (12) is mounted on the X-direction sliding rail (11) in a sliding manner along the X direction;

the multi-well plate assembly (13) is slidably mounted to the Y-direction moving device (12) along the Y direction, and the multi-well plate assembly (13) has a plurality of the sample wells (1311).

3. The light-activated chemiluminescence detection system according to claim 2, wherein the Y-direction movement device (12) comprises a slider (121) and a Y-direction slide rail (122) connected to the slider (121);

the sliding block (121) is mounted on the X-direction sliding rail (11) in a sliding manner;

the perforated plate component (13) is installed on the Y-direction sliding rail (122) in a sliding mode along the Y direction.

4. The light-activated chemiluminescent detection system of claim 3 wherein the multi-well plate assembly (13) comprises a multi-well plate body (131) and a support base (132);

the multi-well plate body (131) is connected with the top surface of the support base (132), and the multi-well plate body (131) is provided with a plurality of sample holes (1311);

the bottom surface of the supporting seat (132) is slidably mounted on the Y-direction sliding rail (122) along the Y direction.

5. The light activated chemiluminescence detection system according to any one of claims 1 to 4, wherein the laser optical path (2) comprises a laser light source, an optical fiber (21) and a collimator (22), and both ends of the optical fiber (21) are respectively connected with the laser light source and the collimator (22).

6. The photoluminescence detection system according to any one of claims 1 to 4, wherein the detection light path (3) comprises a quartz optical window, an optical filter, a photomultiplier tube (31) and a photomultiplier tube housing (32), the quartz optical window and the optical filter are mounted on the photomultiplier tube (31), and the photomultiplier tube housing (32) is mounted outside the photomultiplier tube (31).

7. The photoluminescence detection system according to claim 6, wherein said detection light path (3) further comprises a heat dissipation structure (33), said heat dissipation structure (33) being connected to said photomultiplier (31) through said photomultiplier tube housing (32).

8. The light excited chemiluminescence detection system according to any one of claims 1 to 4, further comprising a housing (4), wherein the mobile platform (1) is mounted in the housing (4), the laser light path (2) and the detection light path (3) are mounted on the top of the housing (4), and the top of the housing (4) has a first light transmission opening (411) corresponding to the position of the laser light path (2), and the top of the housing (4) has a second light transmission opening (412) corresponding to the position of the detection light path (3).

9. The light-activated chemiluminescent detection system of claim 8 wherein the housing (4) comprises a housing (41) and a pick-and-place door (42);

the top of the shell (41) is provided with a taking and placing opening, and the first light transmission opening (411) and the second light transmission opening (412) are arranged on the shell (41);

the taking and placing door (42) is arranged at the taking and placing opening in a sliding mode.

10. The light-activated chemiluminescent detection system of claim 9 wherein the access opening of the housing (41) has a track (413), the access door (42) having a chute (421) that mates with the track (413).