CN215218174U

CN215218174U - Sampling device and drip dyeing instrument

Info

Publication number: CN215218174U
Application number: CN202120732733.XU
Authority: CN
Inventors: 龚小勇; 谭瑞政
Original assignee: Baisheng Guangzhou Biological Products Co ltd
Current assignee: Baisheng Guangzhou Biological Products Co ltd
Priority date: 2021-04-09
Filing date: 2021-04-09
Publication date: 2021-12-17
Anticipated expiration: 2031-04-09

Abstract

The utility model provides a sampling device and drip dyeing appearance, sampling device includes sampling needle and drive unit, the sampling needle includes inner tube and outer tube, the inner tube is used for absorbing the reagent, the material of inner tube and outer tube is electrically conductive metal material, the outer pipe box is established on the inner tube, it has insulating medium to fill between outer tube and the inner tube, insulating medium separates inner tube and outer tube, inner tube, outer tube and insulating medium constitute electric capacity, drive unit includes the mount pad, the sampling needle sets up on the mount pad, drive unit is used for driving the sampling needle and removes. By forming the capacitor structure, when the sampling needle is contacted with the liquid level, the capacitance of the sampling needle jumps suddenly, so that the moving speed of the sampling needle can be controlled according to capacitance signals, the depth of the sampling needle immersed into the liquid level is reduced, and the liquid hanging phenomenon of the sampling needle can be reduced by the sampling device.

Description

Sampling device and drip dyeing instrument

Technical Field

The utility model relates to a drip and dye appearance research field, especially a sampling device and drip and dye appearance.

Background

Morphological observation of tissue samples is always the main work of pathological diagnosis, and the traditional artificial hematoxylin-eosin staining (HE staining) has the disadvantages of early development time, mature staining process, unstable staining, easy artificial influence and cross contamination. With the development of diagnosis level and remote pathology consultation, the requirement on HE high-definition dyeing is more and more, and the original dip dyeing type dyeing instrument is difficult to meet the requirement. The dip dyeing is to dye different tissue samples in the same staining solution, so that the problem of cross contamination between tissues and the staining solution exists, and the staining solution is used for a long time, so that the effect of the staining solution is weakened along with the prolonging of the time, and the defects are obvious.

Drop-staining instruments are currently available, in which a staining solution is drawn up through a sampling needle and added drop-wise separately for each tissue sample. The problem of cross contamination between tissues and a staining solution can be avoided by single drop staining, and reagents added dropwise each time are stock solution reagents, so that the HE staining quality can be kept uniform and stable, the interference of human factors is avoided, and the development trend of the HE staining is met. However, the prior sampling needle is generally directly inserted into the bottom of the reagent tube for liquid suction during sampling, the sampling needle is submerged into the liquid level, and a part of reagent remains on the outer surface of the sampling needle after sampling, so that the liquid hanging phenomenon is generated, and the problems of inaccurate liquid feeding, cross contamination and the like are possibly generated.

Disclosure of Invention

The utility model discloses aim at solving one of the technical problem that exists among the prior art at least. Therefore, the utility model provides a sampling device and drip dyeing appearance, sampling device can alleviate the hanging liquid phenomenon of sampling needle.

According to the utility model provides a sampling device, including sampling needle and drive unit, the sampling needle includes inner tube and outer tube, the inner tube is used for absorbing the reagent, the inner tube with the material of outer tube is electrically conductive metal material, the outer tube cover is established on the inner tube, the outer tube with it has insulating medium to fill between the inner tube, insulating medium separates the inner tube with the outer tube, the inner tube the outer tube with insulating medium constitutes electric capacity, drive unit includes the mount pad, the sampling needle sets up on the mount pad, drive unit is used for the drive the sampling needle removes.

According to the utility model provides a sampling device has following technological effect at least: by forming the capacitor structure, when the sampling needle is contacted with the liquid level, the capacitance of the sampling needle jumps suddenly, so that the moving speed of the sampling needle can be controlled according to capacitance signals, the depth of the sampling needle immersed into the liquid level is reduced, and the liquid hanging phenomenon of the sampling needle can be reduced by the sampling device.

According to some embodiments of the utility model, the mount pad is provided with step motor, step motor connects the sampling needle, step motor is used for the drive the sampling needle removes in vertical direction.

According to some embodiments of the invention, the drive unit comprises at least two slide rails, each slide rail is arranged side by side along the horizontal direction, and the mounting seat is movably arranged on the slide rail.

According to some embodiments of the utility model, the sampling device includes the measuring pump, the measuring pump includes the liquid pipe, the liquid pipe with the sampling needle intercommunication, the measuring pump is used for drawing reagent.

According to the utility model provides a drip dyeing instrument, include the utility model provides a sampling device.

According to the utility model provides a drip dyeing instrument has following technological effect at least: through using the utility model provides a sampling device can alleviate the hanging liquid phenomenon of sampling needle.

According to some embodiments of the utility model, drip and dye the appearance and include the reagent frame, the reagent frame is provided with a plurality of and is used for holding the kit of reagent.

According to some embodiments of the invention, the reagent rack comprises a cooling module, the cooling module is provided with a runner for the flow of cooling liquid, the cooling module is used for controlling the temperature of the reagent.

According to some embodiments of the utility model, the drop dyeing instrument includes the sample case, the sample case is used for placing the slide frame, and the slide frame is used for laying the slide, and the slide is used for bearing the weight of the tissue sample.

According to the utility model discloses a some embodiments, the heating groove has been seted up to the sample case, the heating groove is used for placing the slide frame, the sample case can heat the tissue sample.

According to some embodiments of the utility model, drip and dye the appearance and include the outer container, sampling device sets up in the outer container.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

fig. 1 is an axial view of a drop dyeing apparatus according to the present invention;

FIG. 2 is a schematic view of a sampling needle provided in accordance with the present invention;

fig. 3 is an isometric illustration of a reagent rack provided in accordance with the present invention;

fig. 4 is an axis view of a sample box according to the present invention.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated with respect to the orientation description, such as up, down, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, a plurality of means are one or more, a plurality of means are two or more, and the terms greater than, less than, exceeding, etc. are understood as not including the number, and the terms greater than, less than, within, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless there is an explicit limitation, the words such as setting, installation, connection, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in combination with the specific contents of the technical solution.

According to the utility model provides a sampling device, including sampling needle 1 and drive unit, sampling needle 1 includes inner tube 11 and outer tube 12, inner tube 11 is used for absorbing the reagent, the material of inner tube 11 and outer tube 12 is electrically conductive metal material, outer tube 12 cover is established on inner tube 11, it has insulating medium 13 to fill between outer tube 12 and the inner tube 11, insulating medium 13 separates inner tube 11 and outer tube 12, inner tube 11, outer tube 12 and insulating medium 13 constitute electric capacity, drive unit includes mount pad 21, sampling needle 1 sets up on mount pad 21, drive unit is used for driving sampling needle 1 and removes.

Because the liquid level detection function is not equipped with to traditional all kinds of sampling needles 1, generally the disect insertion reagent socle portion takes a sample to avoid the suction. However, this results in the sampling needle 1 being too deep into the liquid surface, and when the sampling needle 1 is separated from the reagent tube, a part of the reagent remains on the outer wall surface of the sampling needle, resulting in a waste of the reagent. The reagent may flow into the tissue sample during subsequent dripping to cause inaccurate liquid adding, may contaminate other reagents during subsequent sampling, and may form stains on the outer wall surface which are not easy to clean.

According to the utility model provides a sampling device, through forming the electric capacity structure, when sampling probe 1 and liquid level contact, the electric capacity of sampling probe 1 takes place to jump suddenly to can be according to electric capacity signal control sampling probe 1's moving speed, reduce the degree of depth that sampling probe 1 submerged the liquid level, reduce because of the hanging liquid that sampling probe 1 too much leads to under deepening the liquid level, wash incompletely, reagent cross contamination scheduling problem, very big improvement erect accurate nature, the security of application of sample mode.

It will be appreciated that the insulating medium 13 may also serve other functions than barrier insulation. For example, in some embodiments, the insulating medium 13 adhesively secures the outer tube 12 to the inner tube 11. At this time, the insulating medium 13 may be insulating potting adhesive or the like, the outer tube 12 is first positioned, and then the insulating medium 13 is poured between the outer tube 12 and the inner tube 11, and the insulating medium 13 plays a role in fixing after being solidified.

In some embodiments, the sampling needles 1 are arranged in a group of two, and the mounting seat 21 is provided with a plurality of sampling needles, so as to meet the requirements of batch sampling and batch dyeing.

According to some embodiments of the utility model, mount pad 21 is provided with step motor, and step motor connects sampling probe 1, and step motor is used for driving sampling probe 1 and removes in vertical direction. The displacement of the sampling needle 1 can be more accurately controlled by using the stepping motor, and the sampling needle 1 is further prevented from being too deep into the liquid level. In some embodiments, the driving unit includes at least two slide rails 22, each slide rail 22 is disposed side by side in a horizontal direction, and the mounting base 21 is movably disposed on the slide rail 22. If mount pad 21 only sets up on a slide rail, horizontal and vertical deviation easily take place for the gesture of mount pad 21, and then lead to sampling needle 1 skew, through setting up two at least slide rails 22, a plurality of slide rails 22 are fixed a position and are led to mount pad 21 jointly, can improve the gesture stability and the accuracy nature of mount pad 21, and the life-span is longer, has reduced the maintenance time.

It is understood that the above is only a partial implementation manner of the driving unit, and the driving unit may also implement the movement of the sampling needle 1 in the horizontal direction and the vertical direction by adopting various transmission manners such as belt transmission, chain transmission, rack transmission, motor transmission, and the like. Similarly, the stepping motor can be replaced by a servo motor or the like to realize similar driving functions.

According to the utility model discloses a some embodiments, sampling device includes the measuring pump, and the measuring pump includes liquid pipe 14, and liquid pipe 14 communicates with sampling needle 1, and the measuring pump is used for drawing reagent. The metering pump can accurately suck a certain volume of liquid, thereby ensuring the repeatability of the pipetting process. For example, the metering pump can be an injection metering pump, in some embodiments, the sampling amount of the injection metering pump is 10ul-2000ul, and the sampling precision CV value can be controlled below 5%.

According to the utility model provides a drip dyeing instrument, through using the utility model provides a sampling device can improve the sample precision of dripping dyeing instrument.

According to some embodiments of the utility model, drip and dye the appearance and include reagent frame 3, reagent frame 3 is provided with a plurality of and is used for holding the kit 31 of reagent. Different reagents are respectively stored in different reagent boxes 31 without mutual influence. When the drop-staining apparatus is used for HE staining, the kit 31 may be used to store reagents such as dewaxing solution, absolute ethanol, purified water, eosin, hematoxylin, differentiation solution, anti-blue solution, and clearing solution. It can be understood that the reagent kit 31 can have various volume specifications, such as 15ml, 30ml, 60ml, etc., the reagent kit 31 with various specifications can be better adapted to different reagents, if a certain reagent is used in a smaller amount during dyeing, the reagent kit 31 with a small volume can be used for containing, and the volume utilization rate of the reagent rack 3 is improved. Moreover, the reagent kit 31 can be detachably mounted on the reagent rack 3, so that different reagent racks 3 can be combined according to different experimental requirements, and the practicability of the reagent rack 3 is improved.

In some embodiments, the reagent rack 3 is further provided with a needle washing groove 33, the needle washing groove 33 contains a cleaning solution, and the sampling needle 1 can be cleaned in the needle washing groove 33, so that the risk of cross contamination is reduced.

In some embodiments, the reagent rack 3 comprises a cooling module 32, the cooling module 32 being provided with a flow channel for a cooling liquid to flow, the cooling module 32 being used to control the temperature of the reagent. For example, as shown in the embodiment of fig. 3, the cooling module 32 includes a cooling stage which is hollow, a curved coil is disposed at the bottom of the cooling stage, one end of the coil is connected to a liquid inlet of the external cooling device, the other end of the coil is connected to a liquid outlet of the external cooling device, and the external cooling device controls the low-temperature cooling liquid to circulate in the coil. The reagent cartridge 31 is placed on a cooling stage, and the reagents in the reagent cartridge 31 are cooled by heat conduction of the coolant. Since the process of the staining experiment can generally reach 4-5 hours or even longer, the cooling module 32 can keep the reagent in the optimal refrigeration condition all the time during the experiment, thereby prolonging the service life of the reagent and maintaining the titer.

According to some embodiments of the utility model, the drop dyeing instrument includes sample box 4, and sample box 4 is used for placing the slide frame, and the slide frame is used for laying the slide, and the slide is used for bearing the weight of the tissue sample. In some embodiments, the slide rack is provided with vertical mounting slots, and the slides are placed on the slide rack using a slide clamp as disclosed in the co-pending new patent CN 210787421U-new slide mounting configuration, and the slide clamp is inserted onto the slide rack, thereby placing the slides in a vertical fashion and increasing the load bearing density of the specimen box 4.

In some embodiments, the sample chamber 4 defines a heating channel for receiving a slide rack, and the sample chamber 4 is capable of heating the tissue sample. Put the slide frame into the heating tank, sample case 4 heats the sample and keeps warm, improves the dyeing effect. For example, as shown in the embodiment of fig. 4, the sample box 4 is heated in a water bath manner, the sample box 4 further includes a temperature probe 41, a drainage system, a communication system, a power supply system, and the like, the temperature probe 41 is disposed at the bottom of the heating tank, and the heating tank and the temperature probe 41 can perform accurate temperature control according to specific experimental conditions to ensure dyeing quality. The drainage system comprises a drainage port 42, wherein the drainage port 42 is divided into a clear liquid drainage port and a waste liquid drainage port, and is automatically controlled by a program, so that waste liquid can be collected and treated separately. Handles which accord with human engineering mechanics are respectively designed on the left and the right of the sample box 4, so that the sample box is convenient to carry.

According to some embodiments of the utility model, drip and dye the appearance and include the outer container, sampling device sets up in the outer container. The outer container can protect each inside structure, avoids dust, impurity etc. in the environment to produce the interference to the experiment. For example, as shown in the embodiment of fig. 1, the outer box includes a top cover 61 capable of being opened and closed in a turning manner, a sliding door 62 capable of being opened and closed in a sliding manner, and a side frame 63 located at the side, wherein the sliding door 62 and the top cover 61 are closed to block dust at ordinary times, and the sliding door 62 and the top cover 61 can be opened to facilitate operation when necessary. The top cover 61 and the side frames 63 are made of high-transparency and high-strength resin glass, so that the operation state inside the drop dyeing instrument can be observed conveniently, and the personal safety can be guaranteed to the maximum extent by the high-strength resin glass on the premise of guaranteeing wide visual field.

The drip stainer provided according to the present invention is described in detail with a specific embodiment with reference to fig. 1, fig. 2, fig. 3, and fig. 4. It is to be understood that the following description is illustrative only and is not intended as a specific limitation on the invention.

The stainer comprises an outer box, a sampling device, a reagent rack 3 and a sample box 4, wherein the sampling device, the reagent rack and the sample box are arranged in the outer box, and the stainer is used for hematoxylin-eosin dyeing.

The outer container includes the box and sets up the top cap 61 at the box top, and top cap 61 is articulated with the box. The side of the box is provided with a side frame 63, and the front of the box is provided with a sliding door 62 which can be opened in a sliding way. The top cover 61 and the side frames 63 are made of resin glass with good transparency and high strength.

Sampling device includes sampling probe 1, measuring pump and drive unit, drive unit includes mount pad 21, horizontal migration module and vertical removal module, the horizontal migration module includes two slide rails 22, two slide rails 22 set up side by side along parallel direction, the left side at the box is installed to the one end of slide rail 22, the right side at the box is installed to the other end of slide rail 22, mount pad 21 sets up on slide rail 22, mount pad 21 can follow slide rail 22 and remove about, slide rail 22 can follow the box back-and-forth movement. The vertical moving module comprises a stepping motor, the stepping motor is arranged on the mounting seat 21, the sampling needle 1 is connected with the stepping motor, and the stepping motor drives the sampling needle 1 to move up and down along the vertical direction.

Sampling device sets up 16 sampling needles 1 altogether, sampling needle 1 sets up with the array form, the interval between the sampling needle 1 can be adjusted at the allowed range, sampling needle 1 includes inner tube 11 and outer tube 12, inner tube 11 is used for absorbing the reagent, the material of inner tube 11 and outer tube 12 is electrically conductive metal material, outer tube 12 cover is established on inner tube 11, it has insulating medium 13 to fill between outer tube 12 and the inner tube 11, insulating medium 13 separates inner tube 11 and outer tube 12, inner tube 11, outer tube 12 and insulating medium 13 constitute the electric capacity. The measuring pump sets up in mount pad 21, and the measuring pump includes liquid pipe 14, and liquid pipe 14 and sampling needle 1 intercommunication, the measuring pump control sampling needle 1 absorb reagent.

The reagent rack 3 is arranged at the bottom of the box body, and the reagent rack 3 comprises a reagent box 31, a cooling module 32 and a needle washing groove 33. The cooling module 32 comprises a cooling carrier, the cooling carrier is hollow, a bent coil is arranged at the bottom of the cooling carrier, one end of the coil is connected with a liquid inlet of an external cooling device, the other end of the coil is connected with a liquid outlet of the external cooling device, and the external cooling device controls low-temperature cooling liquid to flow in the coil in a circulating manner. The reagent cartridge 31 is detachably placed on the cooling stage, and the reagents in the reagent cartridge 31 are cooled by heat conduction of the cooling liquid. The kit 31 comprises specifications of 15ml, 30ml, 60ml and the like, and can be freely combined and assembled, and the kit 31 is used for storing reagents such as dewaxing liquid, absolute ethyl alcohol, purified water, eosin, hematoxylin, differentiation liquid, anti-blue liquid, transparent liquid and the like. The needle washing tank 33 is detachably placed on the cooling stage, and the needle washing tank 33 contains a cleaning liquid for cleaning the sampling needle 1.

Sample case 4 sets up in the bottom of box, and heating bath has been seted up to sample case 4, and sample case 4 is used for placing and water bath heating tissue sample. The sample box 4 heats and preserves the temperature of the sample, and improves the dyeing effect. The sample box 4 further comprises a temperature probe 41, a drainage system, a communication system, a power supply system and other components, the temperature probe 41 is arranged at the bottom of the heating groove, and the heating groove and the temperature probe 41 can be accurately controlled in temperature according to specific experimental conditions, so that the dyeing quality is ensured. The drainage system comprises a drainage port 42, wherein the drainage port 42 is divided into a clear liquid drainage port and a waste liquid drainage port, and is automatically controlled by a program, so that waste liquid can be collected and treated separately. Handles which accord with human engineering mechanics are respectively designed on the left and the right of the sample box 4, so that the sample box is convenient to carry.

HE staining was performed as follows:

1: the sampling needle 1 starts a self-cleaning function, and the inner wall and the outer wall of the sampling needle 1 are cleaned in the needle washing groove 33;

2: inputting the number of HE samples to be made;

3: the sampling needle 1 starts a liquid level detection function, confirms the existence of a reagent and automatically identifies the suction of non-liquid (such as air, solid and the like);

4: the sampling needle 1 sucks the set reagent amount, and adds the reagent amount to the sample adding port according to the optimized moving track;

5: staining was performed according to the autonomously developed procedures of deparaffinization, hydration, hematoxylin staining, differentiation, bluing, eosin staining, dehydration, clearing, and the like as shown in the following table.

Step (ii) of	Dyeing time	Reagent
			Dewaxing (simultaneously 55 ℃ heat preservation)	Three times for 5min	Environment-friendly dewaxing liquid
Hydration of water	2min four times	Anhydrous ethanol
			Nuclear staining	10min	Hematoxylin
Differentiation	1min	Hydrochloric acid alcohol
			Return blue	2min	PBS buffer
CellsPulp dyeing	1min	Eosin
			Dewatering	2min	Anhydrous ethanol
Is transparent	Twice in 2min	Environment-friendly dewaxing liquid

When the sampling needle 1 contacts with the liquid level, the capacitance of the sampling needle 1 jumps suddenly, so that the moving speed of the sampling needle 1 can be controlled according to capacitance signals, the sampling needle 1 is controlled to synchronously descend along with the descending of the liquid level and absorb reagents, the depth of the sampling needle 1 submerged into the liquid level is reduced, the problems of liquid hanging, incomplete cleaning, cross contamination of the reagents and the like caused by excessive deep penetration of the sampling needle 1 below the liquid level are reduced, and the accuracy and the safety of the vertical sampling mode are greatly improved.

According to the utility model discloses sampling device, through adopting above design, can realize such some functions at least: through using the utility model provides a sampling device can improve the sample precision of dripping the appearance of dyeing.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples" or the like mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A sampling device, comprising:

the sampling probe (1) comprises an inner tube (11) and an outer tube (12), wherein the inner tube (11) is used for absorbing a reagent, the inner tube (11) and the outer tube (12) are made of conductive metal materials, the outer tube (12) is sleeved on the inner tube (11), an insulating medium (13) is filled between the outer tube (12) and the inner tube (11), the insulating medium (13) separates the inner tube (11) and the outer tube (12), and the inner tube (11), the outer tube (12) and the insulating medium (13) form a capacitor;

the driving unit comprises a mounting seat (21), the sampling needle (1) is arranged on the mounting seat (21), and the driving unit is used for driving the sampling needle (1) to move.

2. The sampling device of claim 1, wherein: the mounting seat (21) is provided with a stepping motor, the stepping motor is connected with the sampling needle (1), and the stepping motor is used for driving the sampling needle (1) to move in the vertical direction.

3. A sampling device according to claim 1 or 2, wherein: the driving unit comprises at least two sliding rails (22), each sliding rail (22) is arranged side by side along the horizontal direction, and the mounting seat (21) is movably arranged on each sliding rail (22).

4. A sampling device according to claim 1 or 2, wherein: the sampling device comprises a metering pump, the metering pump comprises a liquid pipe (14), the liquid pipe (14) is communicated with the sampling needle (1), and the metering pump is used for sucking a reagent.

5. A drop dyeing instrument is characterized in that: a stainer comprising a sampling device according to any one of claims 1 to 4.

6. The drop dyeing instrument according to claim 5, characterized in that: the drop dyeing instrument comprises a reagent rack (3), wherein the reagent rack (3) is provided with a plurality of reagent boxes (31) for containing reagents.

7. The drop dyeing instrument according to claim 6, characterized in that: the reagent rack (3) comprises a cooling module (32), the cooling module (32) is provided with a flow channel for flowing of cooling liquid, and the cooling module (32) is used for controlling the temperature of the reagent.

8. The drop dyeing instrument according to claim 5, characterized in that: the drop staining instrument comprises a sample box (4), wherein the sample box (4) is used for placing a slide rack, the slide rack is used for placing slides, and the slides are used for bearing tissue samples.

9. The drop dyeing instrument according to claim 8, characterized in that: heating grooves are formed in the sample box (4) and used for placing the slide rack, and the sample box (4) can heat the tissue sample.

10. The drop dyeing instrument according to claim 5, characterized in that: the drop dyeing instrument comprises an outer box (6), and the sampling device is arranged in the outer box (6).