CN215181182U - Microscopic imaging device - Google Patents
Microscopic imaging device Download PDFInfo
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- CN215181182U CN215181182U CN202121201244.8U CN202121201244U CN215181182U CN 215181182 U CN215181182 U CN 215181182U CN 202121201244 U CN202121201244 U CN 202121201244U CN 215181182 U CN215181182 U CN 215181182U
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Abstract
The utility model provides a microscopic imaging device, include: the device comprises an object stage, a magnifying assembly, an imaging assembly, a plurality of miniature cameras and an image processing module. The utility model discloses under the fixed circumstances of field of vision, through the image that once forms images and obtain a plurality of imaging surfaces, guarantee to carry out image fusion's image content, position and image size equivalent unanimity, reduce the acquisition time of waiting to fuse the image greatly to cooperate effectual depth of field to fuse the algorithm, be used for the rate of accuracy that the depth of field that improves fuses.
Description
Technical Field
The utility model relates to a microscopic imaging field, concretely relates to microscopic imaging device.
Background
Due to the limitation of an optical principle, the microscopic imaging system has the condition that the resolution and the depth of field range are limited, and the high resolution means that the depth of field is shallow. When a sample with depth feeling is detected, and the depth span of the sample exceeds the depth of field of a microscope, objects at different distances in the same scene cannot be imaged clearly at the same time, namely the multi-focal-plane problem. In biological and medical research and application, the sample is usually required to be subjected to thin section treatment, so that observation and clear imaging can be carried out under a microscope, and when the sample structure cannot be modified for thick sections or body fluid visible component detection, living cell culture and the like, the traditional microscope cannot meet the requirement of single clear imaging.
The traditional depth-of-field fusion methods are generally a zoom method, a variable aperture method, a defocusing method, a depth-of-field superposition method and the like, and although images with ultra depth of field can be obtained, the focal length needs to be changed for many times and the images need to be collected. At present, the modes for obtaining multi-focal plane images are all manual focusing or rely on a motor to drive an objective table to move up and down or a piezoelectric objective actuator to drive an objective lens to move so as to adjust the distance between the objective lens and a sample, namely, the focal length is adjusted, but the modes are limited by a mechanical structure, the modes spend different degrees of time in the focusing process, cannot meet the requirement of rapid focusing, and are difficult to realize real-time depth-of-field fusion in the true sense. At present, there is also a method for realizing real-time fast focusing, in which a liquid lens is added to the rear end surface of an objective lens, and the diopter of the liquid lens is continuously changed within an exposure time of a camera, thereby realizing fast focal length adjustment and possibly realizing real-time on-line depth-of-field fusion. However, this method may cause a change in magnification, which is linear and repeatable in the z-axis direction, and this may increase difficulty in image registration in subsequent image processing, and increase difficulty and calculation time for performing size registration and then fusion on the acquired images of multiple focal planes. ) In addition, for the digital pathological microscopic imaging system, the objective table needs to continuously move in two dimensions, namely scanning, and finally each visual field is spliced into a digital pathological picture, so that a group of pictures shot in a period of changing the diopter of the liquid lens are not the same visual field but deviate along with the movement of the platform, the difficulty, the calculation time and the accuracy of a subsequent depth-of-field fusion algorithm are increased, and the real-time depth-of-field fusion is difficult to realize.
Disclosure of Invention
Therefore, the technical problem to be solved by the present invention is to overcome the problem of too long time for collecting images during depth-of-field fusion.
In a first aspect, the present invention provides a microscopic imaging apparatus, comprising: the objective table is used for bearing a sample; the amplifying assembly is arranged above the objective table; the imaging assembly is arranged above the amplifying assembly and used for receiving the parallel light emitted by the amplifying assembly and converging the parallel light into a point, and the imaging assembly comprises at least two focal planes; the miniature cameras are respectively arranged at the imaging surfaces corresponding to the focal surfaces and shoot images of the imaging surfaces; the upper computer is used for fusing the images of the plurality of imaging surfaces into an image; and the motion control system is used for controlling the object stage to move.
Further, the magnification assembly includes an objective lens.
Further, the light splitting module comprises a barrel mirror, and a central axis of the barrel mirror is collinear with a central axis of the amplifying assembly.
Further, the microscopic imaging device further comprises: a beam splitting module for separating the imaging plane from the central axis; the light splitting module includes: and the light splitting element is arranged above the imaging assembly and corresponds to the converged light of the imaging surface.
Further, the light splitting element comprises a semi-reflecting and semi-transparent mirror or a light splitting prism.
Further, the microscopic imaging device further comprises: the light splitting module is used for separating the imaging surface at the central axis; the light splitting module comprises a light splitting element, parallel rays are arranged in the light splitting module and correspond to at least one focus, and the light splitting element is used for refracting the parallel rays to the side part of the light splitting module; and the light condensing assembly is arranged on the side part of the light splitting module and is used for condensing the refracted parallel light into the imaging surface.
Further, the light splitting element comprises a semi-reflecting and semi-transparent mirror or a light splitting prism; the light condensing assembly includes a barrel mirror.
Further, the miniature camera comprises a CCD camera.
Furthermore, the miniature cameras are respectively arranged at the imaging surfaces corresponding to the upper part and the lower part of each focal plane.
Further, the microscopic imaging device further comprises: the condenser lens is arranged below the objective table; and the reflecting mirror is arranged below the collecting mirror.
The utility model discloses technical scheme has following advantage:
the utility model provides a microscopic imaging device under the fixed condition in field of vision, obtains the image of a plurality of imaging planes through once imaging, guarantees to carry out that image fusion's image content, position and image size equivalent are unanimous, dwindles the acquisition time of treating the fusion image greatly to cooperate effectual depth of field to fuse the algorithm, for the rate of accuracy of the depth of field fusion of improvement shortens the time of depth of field fusion, can realize the fusion of real-time depth of field.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural view of a microscopic imaging apparatus provided in embodiment 1 of the present invention;
fig. 2 is a schematic structural diagram of a spectroscopy module provided in embodiment 1 of the present invention;
fig. 3 is a schematic structural diagram of a spectroscopic element and an imaging assembly provided in embodiment 1 of the present invention;
fig. 4 is a schematic structural diagram of a spectroscopic element and an imaging assembly provided in embodiment 2 of the present invention;
an object stage 101; an amplifying assembly 102; a light splitting module 103;
a miniature camera 104; an image processing module 200; a condenser lens 106; a mirror 107;
a motion control system 108; a slide 105; a light splitting element 110.
Detailed Description
The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Furthermore, the technical features mentioned in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other.
Example 1
As shown in fig. 1, embodiment 1 of the present invention provides a microscopic imaging apparatus, including: the device comprises an object stage 101, a magnifying component 102, an imaging component 111, a plurality of miniature cameras 104 and an upper computer.
The stage 101 is used for carrying a sample.
The magnifying assembly 102 is disposed above the stage 101.
The imaging component 111 is disposed above the amplifying component 102, and is configured to receive the parallel light emitted by the amplifying component 102 and converge into a point, where the imaging component 111 includes at least two focal planes.
The plurality of miniature cameras 104 are respectively arranged at the imaging surfaces corresponding to the focal plane and shoot images of the imaging surfaces.
The upper position is used for fusing the images of the plurality of imaging surfaces into one image.
The utility model provides a microscopic imaging device under the fixed condition in field of vision, obtains the image of a plurality of imaging planes through once imaging, guarantees to carry out that image fusion's image content, position and image size equivalent are unanimous, dwindles the acquisition time of treating the fusion image greatly, cooperates effectual degree of depth of field fusion algorithm for the rate of accuracy of the degree of depth of field fusion of improvement shortens the time of degree of depth of field fusion, can realize real-time degree of depth of field fusion.
In this embodiment, the stage 101 is provided with a slide 105, and the sample is disposed on the slide 105.
In this embodiment, the magnifying assembly 102 comprises an objective lens. The objective lens is a lens group formed by combining a plurality of lenses. The combined use aims to overcome the imaging defects of a single lens and improve the optical quality of the objective lens. The magnification component 102 is configured to magnify the sample image.
In this embodiment, the light splitting module 103 includes a cylindrical mirror, and a central axis of the cylindrical mirror is collinear with a central axis of the amplifying assembly 102.
In this embodiment, the microscopic imaging apparatus further includes: the light splitting module 103 is used for separating the imaging plane from the central axis, so that a plurality of miniature cameras 104 can be arranged, and the miniature cameras 104 can be better arranged.
The light splitting module 103 includes: and a light splitting element 110 disposed above the imaging assembly and corresponding to the converged light of the imaging surface. The light splitting element 110 includes a half-reflecting half-mirror or a light splitting prism. The number of the light splitting elements 110 is equal to the number of the imaging planes minus 1.
As shown in fig. 2 and 3, the focal planes 1,2,3, the number and positions of which are customized, correspond to the imaging planes 1 ', 2 ', 3 '. The beam splitting element 110 in fig. 3 is folded with the optical path of the converging light of the imaging planes 2 ', 3' to separate the imaging planes from the central axis, which facilitates the arrangement of the camera. And then the miniature cameras can be arranged on the imaging surfaces corresponding to the upper part and the lower part of each focal plane respectively. I.e., one miniature camera 104 for each imaging plane. And one micro camera 104 is arranged on the imaging plane of the current focusing plane, so that 2n +1 micro cameras 104 are included in fig. 1.
The barrel mirror has an imaging surface of a current focusing surface, for example, the focal surface 1 may be the current focusing surface, and 1' is the imaging surface of the current focusing surface.
The miniature camera 104 comprises a CCD camera. The CCD is a charge coupled device (charge coupled device) for short, which can convert light into electric charge and store and transfer the electric charge, and can also take out the stored electric charge to change the voltage, so it is an ideal CCD camera element.
In this embodiment, the microscopic imaging apparatus further includes: a collection mirror 106, a mirror 107, and a motion control system 108.
The condenser 106 is arranged below the stage 101, and the condenser 106 is used for condensing light; the reflector 107 is arranged below the condenser 106, and the reflector 107 is used for reflecting light; and the motion control system 108 is configured to control the movement of the stage 101.
In this embodiment, the upper computer includes an image processing module 200, and the depth-of-field fusion algorithm executed by the image processing module 200 is a fusion method commonly known in the prior art.
The utility model provides a microscopic imaging device, when objective table 101 is fixed, light shot to through condensing lens 106 gathering reflector 107 surface reflects to the sample through reflector 107, and the image warp of sample objective enlargies the back, locates to form a plurality of focal planes and corresponding imaging surface at beam splitting module 103, miniature camera 104 gathers and fuses behind the image of imaging surface and forms an image.
The utility model discloses can abandon traditional manual focusing, the focusing movement time that motor drive objective table 101 focusing, piezoelectric objective focusing mode caused consuming time, and especially be fit for being applied to this kind of applied scene of digital pathological scanning, concatenation formation of image, can avoid scanning process field of vision and focus to change simultaneously and increase the problem of the degree of difficulty, calculation time for follow-up image processing. The method can provide original picture materials for subsequent depth-of-field fusion, and provides powerful assistance for realizing real-time depth-of-field fusion.
Example 2
The embodiment 2 of the utility model provides a microscopic imaging device, with embodiment 1 difference lies in, embodiment 2 reforms transform in the inside of formation of image subassembly.
Specifically, the light splitting module includes a light splitting element, which is disposed in the light splitting module and corresponds to the parallel light of the at least one focus, and is configured to refract the parallel light to a side of the light splitting module. The light condensing assembly is arranged on the side part of the light splitting module and used for condensing the refracted parallel light into the imaging surface. The light splitting element comprises a semi-reflecting and semi-transparent mirror or a light splitting prism; the light condensing assembly includes a barrel mirror.
Referring to fig. 4, focal planes 1,2 and 3 are modified to change the optical paths of focal planes 1 and 3, and a light splitting element is arranged in the focal planes to lead out the parallel optical paths to the side of the imaging component and then converge the parallel light into imaging planes 1 'and 3'. This has the advantages that: the distance between the objective lens and the cylindrical lens is long, so that a plurality of light splitting elements can be added, and the light splitting elements are matched with the light condensing assembly, so that a plurality of imaging surfaces can be obtained, and the number of images shot by the miniature camera can be increased; besides, the influence of the light splitting element additionally arranged in the parallel light path on the imaging quality is small, so that the image quality of the obtained imaging surface is high.
The improved place of the embodiment 1 is above the imaging assembly, while the embodiment 2 is improved in the imaging assembly, so that more imaging positions and better imaging quality can be obtained; and further the subsequent depth-of-field fusion effect is better.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made without departing from the scope of the invention.
Claims (10)
1. A microscopic imaging apparatus, comprising:
the objective table is used for bearing a sample;
the amplifying assembly is arranged above the objective table;
the imaging assembly is arranged above the amplifying assembly and used for receiving the parallel light emitted by the amplifying assembly and converging the parallel light into a point, and the imaging assembly comprises at least two focal planes;
the miniature cameras are respectively arranged at the imaging surfaces corresponding to the focal surfaces and shoot images of the imaging surfaces;
the upper computer is used for fusing the images of the plurality of imaging surfaces into an image; and
and the motion control system is used for controlling the object stage to move.
2. A microscopic imaging apparatus according to claim 1,
the magnification assembly includes an objective lens.
3. A microscopic imaging apparatus according to claim 1, wherein the microscopic imaging apparatus further comprises: a light-splitting module,
the light splitting module comprises a cylindrical mirror, and the central axis of the cylindrical mirror is collinear with the central axis of the amplifying assembly.
4. A microscopic imaging apparatus according to claim 3,
a beam splitting module for separating the imaging plane from the central axis;
the light splitting module includes: and the light splitting element is arranged above the imaging assembly and corresponds to the converged light of the imaging surface.
5. A microscopic imaging apparatus according to claim 4,
the light splitting element comprises a semi-reflecting and semi-transparent mirror or a light splitting prism.
6. A microscopic imaging apparatus according to claim 3, further comprising: the light splitting module is used for separating the imaging surface at the central axis;
the light splitting module comprises a light splitting element, parallel rays are arranged in the light splitting module and correspond to at least one focus, and the light splitting element is used for refracting the parallel rays to the side part of the light splitting module;
and the light condensing assembly is arranged on the side part of the light splitting module and is used for condensing the refracted parallel light into the imaging surface.
7. A microscopic imaging apparatus according to claim 6,
the light splitting element comprises a semi-reflecting and semi-transparent mirror or a light splitting prism; the light condensing assembly includes a barrel mirror.
8. A microscopic imaging apparatus according to claim 1,
the miniature camera includes a CCD camera.
9. A microscopic imaging apparatus according to claim 1,
the miniature cameras are respectively arranged on the imaging surfaces corresponding to the upper part and the lower part of each focal surface.
10. The microscopic imaging apparatus according to claim 1, further comprising:
the condenser lens is arranged below the objective table; and
and the reflecting mirror is arranged below the collecting mirror.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121201244.8U CN215181182U (en) | 2021-05-31 | 2021-05-31 | Microscopic imaging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121201244.8U CN215181182U (en) | 2021-05-31 | 2021-05-31 | Microscopic imaging device |
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| Publication Number | Publication Date |
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| CN215181182U true CN215181182U (en) | 2021-12-14 |
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| CN202121201244.8U Active CN215181182U (en) | 2021-05-31 | 2021-05-31 | Microscopic imaging device |
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2021
- 2021-05-31 CN CN202121201244.8U patent/CN215181182U/en active Active
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