CN215162820U - 3D cell dynamic culture instrument - Google Patents
3D cell dynamic culture instrument Download PDFInfo
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- CN215162820U CN215162820U CN202121491964.2U CN202121491964U CN215162820U CN 215162820 U CN215162820 U CN 215162820U CN 202121491964 U CN202121491964 U CN 202121491964U CN 215162820 U CN215162820 U CN 215162820U
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Abstract
The utility model provides a 3D cell dynamic culture instrument, which comprises a first stabilizing plate, a rotating gear, a stirrer, a culture plate and a speed regulating motor; the upper end surface of the first stabilizing plate is provided with a plurality of positioning supporting seats, the lower end surface of the first stabilizing plate is provided with a plurality of limiting seats, and the positioning supporting seats and the limiting seats are communicated through holes; the lower end of the rotating gear is inserted into the positioning support seat, a plurality of rotating gears are arranged in one-to-one correspondence with the positioning support seat, and adjacent rotating gears are meshed with each other; the upper end of the stirrer penetrates through the limiting seat and the through hole to be connected with the rotating gear; the culture plate is provided with culture holes, the lower end of the stirrer can extend into the culture holes, and the first stabilizing plate covers the culture plate; the output end of the speed regulating motor is connected with the rotating gear to drive the rotating gear to rotate. 3D cell dynamic culture appearance solved the difficult homogenization's of culture condition in every culture hole among the culture vessel problem, realized that the downthehole, the downthehole cell ball size homogenization of different cultivations of same culture.
Description
Technical Field
The utility model belongs to the technical field of cell culture, especially, relate to a 3D cell dynamic culture appearance.
Background
With the progress of science and technology and biology, in vitro suspension culture of cells becomes a common and important technical means in cell biology research methods, and is also an important technical means for organoids, multicellular spheroids, tumor models, skin models, stem cells, bioengineering and drug screening. During the cell culture process, the microenvironment in which the cells are cultured plays a key role in cell growth, and for example, the gas environment, especially the oxygen concentration and the carbon dioxide content, are essential in the process of cell physiological metabolism. Currently, 3D cell culture in laboratories mainly employs 3D hanging drop cell culture technology and Matrigel in vitro culture technology, which is characterized by static culture. At present, the 3D culture technology of cells in China is not widely applied, and the reason is that the culture condition of each hole is not easy to be uniform, so that the sizes of cell balls in each hole are inconsistent, and the sizes of the cell balls between the holes are inconsistent. Effective means for drug screening and the like cannot be carried out. In addition, the method has the problems of insufficient operation in the aspect of simulating the living environment in the cell body, high production cost, high labor intensity and the like.
Disclosure of Invention
In view of this, in order to overcome above-mentioned problem, the utility model provides a 3D cell dynamic culture appearance has solved the difficult homogenization's of culture condition in every culture hole among the culture vessel problem, realizes the homogenization of cell ball size in every culture hole, and the homogenization of cell ball size in the different culture holes to carry out high flux screening research for organoid, multicellular ball, tumor model, skin model and provide effective support.
In order to achieve the above purpose, the technical scheme of the utility model is realized like this:
3D cell dynamic culture apparatus comprising:
the upper end surface of the first stabilizing plate is distributed with a plurality of positioning supporting seats, the lower end surface of the first stabilizing plate is distributed with a plurality of limiting seats which are coaxial with the positioning supporting seats, and the coaxial positioning supporting seats and the limiting seats are communicated through holes;
the rotary gear comprises a gear body, a gear upper part arranged at the upper end of the gear body and a gear lower part arranged at the lower end of the gear body; the lower part of the gear is movably inserted into the positioning support seat; a plurality of rotary gears are arranged and correspond to the positioning supporting seats one by one, and gear bodies of adjacent rotary gears are meshed with each other;
the stirrer comprises a stirring shaft and a stirring blade arranged at the lower end of the stirring shaft, and the upper end of the stirring shaft penetrates through the limiting seat and the through hole to be connected with the rotating gear; the stirrers are arranged in a one-to-one correspondence manner with the rotating gears;
the culture plate is provided with culture holes, and one end of the stirrer provided with the stirring blade can extend into the culture holes; the culture holes are arranged in a one-to-one correspondence manner with the stirrers; the first stabilizing plate is detachably covered on the culture plate;
the speed regulating motor is supported by a motor support, and the output end of the speed regulating motor is connected with the upper part of the gear to drive the rotating gear to rotate.
Further, still include the second stabilising plate, the parallel suspension of second stabilising plate is located the top of first stabilising plate, be equipped with the location preformed hole on the second stabilising plate, the location preformed hole has a plurality ofly, and is a plurality of location supporting seat one-to-one sets up, the mobilizable inserting in gear upper portion is located in the location preformed hole.
Furthermore, the lower end face of the first stabilizing plate is of a groove structure which is sunken upwards, the limiting seat is arranged in the groove, and the first stabilizing plate covers the culture plate through the groove structure.
Furthermore, a limiting protruding part is arranged at the position, located in the limiting seat, of the stirring shaft.
Further, the rotating speed of the speed regulating motor is 0-200 r/min.
Further, the culture plate is provided with 6-96 culture holes.
Compared with the prior art, the 3D cell dynamic culture instrument of the utility model has the following advantages:
(1) the utility model discloses a 3D cell dynamic culture appearance pass through the gear of the output power of buncher and then drive the agitator and constantly stir in the culture hole of culture plate, make the cell be in the continuous flow state to guarantee sufficient gas exchange and impel the cell to the absorption of oxygen, carbon dioxide and culture solution, reach the purpose of dynamic culture, improve the formation and the survival rate of cell; meanwhile, the consistency of the rotating speed of each stirrer and the rotating speed of the adjustable motor is achieved through the coupling connection of the gear body and the gear body, the stirring speed and the culture conditions in the culture holes are completely consistent, the cell balls in each culture hole are ensured to be uniform in size, and the cell balls in different culture holes are uniform in size;
(2) 3D cell dynamic culture appearance can be organoid, multicellular ball, tumor model, skin model, 3D cultivates such as stem cell and provides a microenvironment that is closer to the in vivo living condition more, also for early drug development, toxicity inspection, cancer tissue drug effect test, protein pharmacy, shorten stem cell directional differentiation time, stem cell research and tissue engineering, regenerative medicine, virus infection model establishment etc. provide effective support.
Drawings
The accompanying drawings, which form a part hereof, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without undue limitation. In the drawings:
fig. 1 is a schematic structural diagram of a 3D cell dynamic culture apparatus according to an embodiment of the present invention;
fig. 2 is a schematic side view of a 3D cell dynamic culture apparatus according to an embodiment of the present invention;
fig. 3 is a schematic diagram of the internal structure of a 3D cell dynamic culture apparatus according to an embodiment of the present invention;
fig. 4 is a schematic structural view of a rotary gear according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of an agitator according to an embodiment of the present invention.
Description of reference numerals:
1-a first stabilising plate; 2, positioning a supporting seat; 3-a limiting seat; 4-a second stabilising plate; 5-positioning a prepared hole; 6-a rotating gear; 7-a gear body; 8-gear upper part; 9-gear lower part; 10-a stirrer; 11-a stirring shaft; 12-stirring blade; 13-a limit projection; 14-a culture plate; 15-speed regulating motor.
Detailed Description
It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
As shown in fig. 1 to 5, a 3D cell dynamic culture apparatus includes:
the lower end face of the first stabilizing plate 1 is of an upwards-sunken groove structure, a plurality of positioning supporting seats 2 are distributed on the upper end face of the first stabilizing plate 1, a plurality of limiting seats 3 which are coaxial with the positioning supporting seats 2 are distributed on the inner face of the groove of the first stabilizing plate 1, and the coaxial positioning supporting seats 2 and the limiting seats 3 are communicated through holes; the cross sections of the positioning supporting seat 2 and the limiting seat 3 are both circular;
the second stabilizing plate 4 is suspended above the first stabilizing plate 1 in parallel, a plurality of positioning preformed holes 5 are formed in the second stabilizing plate 4, and the positioning preformed holes 5 are arranged in one-to-one correspondence with the positioning supporting seats 2;
the rotary gear 6 comprises a gear body 7, a gear upper part 8 arranged at the upper end of the gear body 7 and a gear lower part 9 arranged at the lower end of the gear body 7; the lower gear part 9 is inserted in the positioning support seat 2, and a gap is formed between the lower gear part and the inner side wall of the positioning support seat 2, namely, the rotating gear 6 is not fixedly connected with the positioning support seat 2, the lower gear part 9 can rotate in the positioning support seat 2 under the action of external force, and the positioning support seat 2 mainly plays a role in supporting the rotating gear 6; the upper part 8 of the gear is inserted into the positioning preformed hole 5, a gap is formed between the upper part and the side wall of the positioning preformed hole 5, and the positioning preformed hole 5 mainly plays a role in preventing the rotary gear 6 from shaking up and down or horizontally; a plurality of rotating gears 6 are arranged in one-to-one correspondence with the plurality of positioning supporting seats 2, and gear bodies 7 of adjacent rotating gears 6 are meshed with each other;
the stirrer 10 comprises a stirring shaft 11 and a stirring blade 12 arranged at the lower end of the stirring shaft 11, the upper end of the stirring shaft 11 penetrates through the limiting seat 3 and the through hole to be connected with the rotating gear 6, a limiting protruding part 13 is arranged at the position, located in the limiting seat 3, of the stirring shaft 11, a gap is formed between the limiting protruding part 13 and the inner side wall of the limiting seat 3, when the stirrer 10 rotates, the limiting seat 3 cannot limit the rotation of the stirrer 10, and the limiting seat 3 mainly plays a role in preventing the stirrer 10 from shaking; a plurality of stirrers 10 are arranged corresponding to the plurality of rotating gears 6 one by one;
the culture plate 14 is provided with 6-96 culture holes, and one end of the stirrer 10 provided with the stirring blade 12 can extend into the culture holes; the culture holes are arranged in one-to-one correspondence with the stirrers 10; the first stabilizing plate 1 is detachably covered on the culture plate 14 through a groove structure;
the rotating speed of the speed regulating motor 15 is 0-200 r/min, the speed regulating motor 15 is supported and suspended above the second stabilizing plate 4 by a motor support, and the output end of the speed regulating motor 15 is connected with the upper portion 8 of the gear, so that the rotating gear 6 can be driven to rotate, and the stirrer 10 is further driven to rotate.
And (3) adopting the 3D cell dynamic culture instrument to culture the cells:
the culture plate was a 12-well cell culture plate, and 3ml of a liquid (a mixture of brain cells and a culture medium obtained by differentiation of stem cells) was added to the culture well. Covering the first stabilizing plate on the culture plate, wherein the lower end of the stirrer is positioned in the culture hole, putting the assembled 3D cell dynamic culture instrument into a carbon dioxide cell incubator, setting the rotating speed to be 60rpm, supplementing cell culture medium every 2 days, and culturing for 21 days to obtain uniform cell microspheres with the diameter of about 2 mm.
Under the same conditions, only small cell spheres with a diameter of 0.5mm at most can be obtained by the Matrigel in vitro culture technology, and the cell spheres are not uniform from hole to hole.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
- 3D cell dynamic culture apparatus, comprising:the upper end surface of the first stabilizing plate is distributed with a plurality of positioning supporting seats, the lower end surface of the first stabilizing plate is distributed with a plurality of limiting seats which are coaxial with the positioning supporting seats, and the coaxial positioning supporting seats and the limiting seats are communicated through holes;the rotary gear comprises a gear body, a gear upper part arranged at the upper end of the gear body and a gear lower part arranged at the lower end of the gear body; the lower part of the gear is movably inserted into the positioning support seat; a plurality of rotary gears are arranged and correspond to the positioning supporting seats one by one, and gear bodies of adjacent rotary gears are meshed with each other;the stirrer comprises a stirring shaft and a stirring blade arranged at the lower end of the stirring shaft, and the upper end of the stirring shaft penetrates through the limiting seat and the through hole to be connected with the rotating gear; the stirrers are arranged in a one-to-one correspondence manner with the rotating gears;the culture plate is provided with culture holes, and one end of the stirrer provided with the stirring blade can extend into the culture holes; the culture holes are arranged in a one-to-one correspondence manner with the stirrers; the first stabilizing plate is detachably covered on the culture plate;the speed regulating motor is supported by a motor support, and the output end of the speed regulating motor is connected with the upper part of the gear to drive the rotating gear to rotate.
- 2. The 3D cell dynamic culture apparatus according to claim 1, wherein: still include the second stabilising plate, the parallel suspension of second stabilising plate is located the top of first stabilising plate, be equipped with the location preformed hole on the second stabilising plate, the location preformed hole has a plurality ofly, and is a plurality of location supporting seat one-to-one sets up, the mobilizable inserting in gear upper portion is located in the location preformed hole.
- 3. 3D cell dynamic culture apparatus according to claim 1 or 2, characterized in that: the lower end face of the first stabilizing plate is of a groove structure which is sunken upwards, the limiting seat is arranged in the groove, and the first stabilizing plate covers the culture plate through the groove structure.
- 4. 3D cell dynamic culture apparatus according to claim 1 or 2, characterized in that: and the stirring shaft is provided with a limiting protruding part at the position in the limiting seat.
- 5. 3D cell dynamic culture apparatus according to claim 1 or 2, characterized in that: the rotating speed of the speed regulating motor is 0-200 r/min.
- 6. 3D cell dynamic culture apparatus according to claim 1 or 2, characterized in that: the culture plate is provided with 6-96 culture holes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121491964.2U CN215162820U (en) | 2021-07-01 | 2021-07-01 | 3D cell dynamic culture instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121491964.2U CN215162820U (en) | 2021-07-01 | 2021-07-01 | 3D cell dynamic culture instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215162820U true CN215162820U (en) | 2021-12-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121491964.2U Active CN215162820U (en) | 2021-07-01 | 2021-07-01 | 3D cell dynamic culture instrument |
Country Status (1)
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|---|---|
| CN (1) | CN215162820U (en) |
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2021
- 2021-07-01 CN CN202121491964.2U patent/CN215162820U/en active Active
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Effective date of registration: 20211231 Address after: 300220 423, building 32, No. 8, Nujiang Road, Hexi District, Tianjin Patentee after: Yimicron (Tianjin) Technology Co.,Ltd. Address before: E01-101, Zunhua business center, 985 Dagu South Road, Hexi District, Tianjin 300299 Patentee before: Tianjin xiangtian Technology Co.,Ltd. Patentee before: Tianjin hemiao Technology Co., Ltd |