CN215162814U

CN215162814U - Culture dish convenient to remove umbilical cord mesenchymal stem cells

Info

Publication number: CN215162814U
Application number: CN202121299851.2U
Authority: CN
Inventors: 倪琳
Original assignee: Tianjin Changhe Biological Technology Co ltd
Current assignee: Tianjin Changhe Biological Technology Co ltd
Priority date: 2021-06-10
Filing date: 2021-06-10
Publication date: 2021-12-14
Anticipated expiration: 2031-06-10

Abstract

The utility model discloses a culture dish convenient to remove umbilical cord mesenchymal stem cell relates to culture dish technical field, for the current umbilical cord mesenchymal stem cell culture among the solution prior art need wash clean back, picks the blood vessel with scissors tweezers, peels out the Fahrenheit glue tissue of the inside, and single traditional culture dish can't provide the problem of a comparatively good separation environment. The inside of isolated culture dish is provided with the cell culture chamber, and the inside in cell culture chamber is provided with washs the separation ramp, it sets up the integral type structure with the isolated culture dish to wash the separation ramp, and washs the inclination of separation ramp and be 60, the outside of isolated culture dish is provided with the sealing ring, and sealing ring and isolated culture dish fixed connection, the top of isolated culture dish is provided with transparent closing cap, and transparent closing cap passes through the sealing ring and is connected with the isolated culture dish.

Description

Culture dish convenient to remove umbilical cord mesenchymal stem cells

Technical Field

The utility model relates to a culture dish technical field specifically is a culture dish convenient to remove umbilical cord mesenchymal stem cell.

Background

A petri dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic. The culture dish is basically made of two types of materials, mainly plastic and glass, the glass can be used for plant materials, microbial culture and animal cell adherent culture, and can also be used for umbilical cord mesenchymal stem cell culture, the umbilical cord mesenchymal stem cells have wide clinical application prospects, the human umbilical cord mesenchymal stem cells can be successfully amplified by applying an inactivated umbilical cord serum culture system, the cultured cells have the basic characteristics of the mesenchymal stem cells, and theoretical bases are provided for establishment of a mesenchymal stem cell bank and clinical application.

However, after the conventional umbilical cord mesenchymal stem cells need to be washed clean, blood vessels are removed by scissors and tweezers, and Fahrenheit jelly tissues in the blood vessels are stripped, so that a relatively good separation environment cannot be provided by a single traditional culture dish; therefore, the existing requirements are not met, and a culture dish convenient for moving the umbilical cord mesenchymal stem cells is provided for the culture dish.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a culture dish convenient to remove umbilical cord mesenchymal stem cell to solve the current umbilical cord mesenchymal stem cell culture that proposes in the above-mentioned background art and need wash clean back, pick the blood vessel with scissors tweezers, peel out the Fahrenheit glue tissue of the inside, single traditional culture dish can't provide the problem of a comparatively good separation environment.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a culture dish convenient to remove umbilical cord mesenchymal stem cell, includes the separation culture dish, the inside of separation culture dish is provided with the cell culture chamber, and the inside in cell culture chamber is provided with washs the separation ramp, it sets up to the integral type structure with the separation culture dish to wash the separation ramp, and the inclination of just wasing the separation ramp is 60, the outside of separation culture dish is provided with sealed ring, and sealed ring and separation culture dish fixed connection.

Preferably, a transparent sealing cover is arranged above the separation culture dish and is connected with the separation culture dish through a sealing ring.

Preferably, a vessel plate groove is formed in the top of the washing and separating slope plate, a cell main plate is arranged in the vessel plate groove, and the cell main plate is connected with the washing and separating slope plate through the vessel plate groove.

Preferably, the two ends of the cell mainboard are provided with loading and unloading bayonets, and the loading and unloading bayonets are of arc structures.

Preferably, both sides of the cleaning and separating slope plate are provided with cleaning liquid tanks.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model discloses a washing separation ramp and separation culture dish set up to the integral type structure, both sides at washing separation ramp all are provided with the washing cistern simultaneously, can move into glass's blood vessel and other cell tissue in the washing cistern cleaning the in-process of separation, conveniently extract complete umbilical cord mesenchymal stem cell tissue like this, and inclination is in order that the in-process cell tissue that washs can together flow into the washing cistern of both sides with the washing liquid in, the umbilical cord mesenchymal stem cell that needs to draw then can hold back at the top of washing separation ramp, after accomplishing the washing separation, take out the cell mainboard, wash the processing to this separation culture dish afterwards, later will have the cell mainboard of stem cell to put into the separation culture dish again can.

Drawings

FIG. 1 is an overall front view of the present invention;

FIG. 2 is a schematic structural view of the separation culture dish of the present invention;

fig. 3 is the schematic view of the sectional structure of the cleaning and separating slope plate of the present invention.

In the figure: 1. separating the culture dish; 2. a cell culture chamber; 3. a transparent cover; 4. sealing the ring; 5. cleaning the separation slope plate; 6. a cell plate; 7. assembling and disassembling the bayonet; 8. a vessel plate groove; 9. and a cleaning liquid tank.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-2, the present invention provides an embodiment: a culture dish convenient for moving umbilical cord mesenchymal stem cells comprises a separation culture dish 1, wherein a cell culture cavity 2 is arranged inside the separation culture dish 1, a cleaning and separating slope plate 5 is arranged inside the cell culture cavity 2, the cleaning and separating slope plate 5 and the separation culture dish 1 are of an integrated structure, the inclination angle of the cleaning and separating slope plate 5 is 60 degrees, the inclination angle is that cell tissues can flow into cleaning liquid grooves 9 at two sides together with cleaning liquid in the cleaning process conveniently, umbilical cord mesenchymal stem cells to be extracted can be intercepted at the top of the cleaning and separating slope plate 5, after cleaning and separating are completed, a cell main plate 6 is taken out, then the separation culture dish 1 is cleaned, then the cell main plate 6 with the stem cells is put into the separation culture dish 1 again, and the outer side of the separation culture dish 1 is provided with a sealing ring 4, and the sealing ring 4 is fixedly connected with the separation culture dish 1, a transparent sealing cover 3 is arranged above the separation culture dish 1, the transparent sealing cover 3 is connected with the separation culture dish 1 through the sealing ring 4, and the transparent sealing cover 3 is covered above the separation culture dish 1 for refrigeration and preservation after cleaning and separation are finished.

Referring to fig. 2, a vessel plate groove 8 is formed in the top of the washing and separating slope plate 5, a cell main plate 6 is arranged inside the vessel plate groove 8, the cell main plate 6 is connected with the washing and separating slope plate 5 through the vessel plate groove 8, the extracted fresh healthy umbilical cord is cut and placed on the cell main plate 6, then the cell main plate 6 is moved into the vessel plate groove 8 in the top of the washing and separating slope plate 5, loading and unloading bayonets 7 are arranged at two ends of the cell main plate 6, the loading and unloading bayonets 7 are of an arc-shaped structure, and the cell main plate 6 is clamped on the loading and unloading bayonets 7 through tweezers during use, so that the operation of taking out and loading the cell main plate 6 is completed.

Referring to fig. 3, both sides of the cleaning and separating slope plate 5 are provided with cleaning liquid tanks 9, the cleaning liquid tanks 9 are located below the slope of the cleaning and separating slope plate 5, and the blood vessels and other cell tissues of the glass can be moved into the cleaning liquid tanks 9 in the process of cleaning and separating, so that the complete umbilical cord mesenchymal stem cell tissue can be conveniently extracted.

The working principle is as follows: when in use, the extracted fresh healthy umbilical cord is cut off and then placed on a cell mainboard 6, both ends of the cell mainboard 6 are provided with loading and unloading bayonets 7, the loading and unloading bayonets 7 are of arc structures, tweezers can be used to clamp the loading and unloading bayonets 7 to complete the operation of taking out and filling the cell mainboard 6, then the cell mainboard 6 is moved into a vessel plate groove 8 at the top of a cleaning and separating slope plate 5, the inclination angle of the cleaning and separating slope plate 5 is 60 degrees, the inclination angle is that the cell tissue and cleaning liquid can flow into cleaning liquid grooves 9 at both sides together in the cleaning process, umbilical cord mesenchymal stem cells to be extracted can be intercepted at the top of the cleaning and separating slope plate 5, after the cleaning and separating are completed, the cell mainboard 6 is taken out, then the separation culture vessel 1 is cleaned, and then the cell mainboard 6 with the stem cells is placed in the separation culture vessel 1 again, and finally, after the cleaning and separation are finished, the transparent sealing cover 3 is covered above the separation culture dish 1 for refrigeration and preservation.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a culture dish convenient to remove umbilical cord mesenchymal stem cell, includes separation culture dish (1), its characterized in that: the inside of separation culture dish (1) is provided with cell culture chamber (2), and the inside of cell culture chamber (2) is provided with washs separation ramp (5), wash separation ramp (5) and separation culture dish (1) and set up to the integral type structure, and the inclination of wasing separation ramp (5) is 60, the outside of separation culture dish (1) is provided with sealing ring (4), and sealing ring (4) and separation culture dish (1) fixed connection.

2. The culture dish for facilitating the movement of umbilical cord mesenchymal stem cells according to claim 1, wherein: a transparent sealing cover (3) is arranged above the separation culture dish (1), and the transparent sealing cover (3) is connected with the separation culture dish (1) through a sealing ring (4).

3. The culture dish for facilitating the movement of umbilical cord mesenchymal stem cells according to claim 1, wherein: the top of washing separation ramp (5) is provided with household utensils board groove (8), the inside in household utensils board groove (8) is provided with cell mainboard (6), and cell mainboard (6) are connected with washing separation ramp (5) through household utensils board groove (8).

4. A culture dish for facilitating moving umbilical cord mesenchymal stem cells according to claim 3, wherein: both ends of the cell mainboard (6) are provided with loading and unloading bayonets (7), and the loading and unloading bayonets (7) are arranged to be of an arc-shaped structure.

5. The culture dish for facilitating the movement of umbilical cord mesenchymal stem cells according to claim 1, wherein: and cleaning liquid tanks (9) are arranged on both sides of the cleaning and separating slope plate (5).