CN215156919U

CN215156919U - T cell CAR molecule expression analysis detection kit

Info

Publication number: CN215156919U
Application number: CN202120420544.9U
Authority: CN
Inventors: 许恩顺; 梁宁孝; 王海珍; 何奕辉
Original assignee: Nanjing Diji Biotechnology Co ltd
Current assignee: Nanjing Diji Biotechnology Co ltd
Priority date: 2021-02-25
Filing date: 2021-02-25
Publication date: 2021-12-14
Anticipated expiration: 2031-02-25

Abstract

The utility model relates to a detect reagent box technical field, and disclose a T cell CAR molecule expression analysis detect reagent box, be open-ended kit box body including the top, the same fixed plate of fixedly connected with on the both sides inner wall of kit box body has seted up a plurality of fixed mouths on the fixed plate, has seted up a plurality of standing grooves on the bottom inner wall of kit box body, and has placed same top in standing groove and the fixed mouth and for open-ended reagent bottle, two sliding trays have been seted up at the top of kit box body. The utility model discloses a plurality of removal and the location to sealed lid seal up the reagent bottle, have prevented to appear rocking the medicament that leads to in the reagent bottle easily and has fallen to spill carrying the in-process, give people the condition that brings the loss and appear, very big having made things convenient for the user to carry, have improved the security performance of storing, and is easy and simple to handle, the better use that has made things convenient for medical personnel has satisfied user's needs.

Description

T cell CAR molecule expression analysis detection kit

Technical Field

The utility model relates to a detect reagent box technical field, especially relate to a T cell CAR molecule expression analysis detect reagent box.

Background

Most patients with B cell malignancies, including chronic lymphocytic leukemia, have poor cure rates and prognosis with chemotherapy, targeted therapy, one approach to treat these patients is to genetically modify T cells to target antigens expressed on tumor cells through expression of chimeric antigen receptors, CARs are antigen receptors designed to recognize cell surface antigens in a human leukocyte antigen, independent manner, and attempts to treat these types of patients with genetically modified T cells expressing CARs have been successful to some extent.

Expression of CAR molecules in T cells under treatment is critical to the efficacy of the treatment, and thus the ability to monitor CAR expression is of great importance to CAR-T therapy.

If need carry out the analysis and detection to T cell CAR molecule expression among the prior art, need use multiple medicament, obtain multiunit testing result, need use a plurality of reagent container partial shipment, because of do not possess the function of carrying out sealed to the reagent bottle, the in-process appears rocking and leads to the medicament in the reagent bottle to spill easily, brings the loss for people, carries and stores all inconveniently, can not satisfy user's needs.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving among the prior art if need carry out the analysis and detection to T cell CAR molecule expression, need use multiple medicament, obtain the multiunit testing result, need use a plurality of reagent container partial shipment, because of do not possess the function of sealing up to the reagent bottle, carry the in-process and appear rocking and lead to the medicament in the reagent bottle easily and spill, give people and bring the loss, it is inconvenient all to carry and store, can not satisfy the problem of user's needs, and the T cell CAR molecule expression analysis detect reagent box that provides.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a T cell CAR molecule expression analysis detection kit comprises a kit body with an opening at the top, wherein the inner walls of two sides of the kit body are fixedly connected with the same fixed plate, the fixed plate is provided with a plurality of fixed openings, the inner wall of the bottom of the kit body is provided with a plurality of placing grooves, the placing grooves and the fixed openings are internally provided with the same reagent bottles with the openings at the tops, the top of the kit body is provided with two sliding grooves, the two sliding grooves are internally and respectively connected with sliding plates in a sliding manner, the top ends of the two sliding plates extend to the upper part of the kit body and are fixedly connected with the same connecting plate, the bottom of the connecting plate is fixedly connected with two connecting blocks, the bottom ends of the two connecting blocks extend into the kit body and are fixedly connected with the same connecting rod, the bottom of the connecting rod is fixedly connected with a plurality of sealing covers with openings at the bottoms, and the sealing covers are respectively matched with the corresponding reagent bottles in a sealing manner, the fixed orifices have all been seted up to the both sides of kit box body, and two fixed orifices communicate with corresponding sliding tray respectively, and equal sliding connection has fixed kelly in two fixed orifices.

Preferably, one side that two sliding plates kept away from each other has all been seted up fixed slot, and the one end that two fixed kellies are close to each other clamps with corresponding fixed slot respectively mutually, and the one end that two fixed kellies kept away from each other all extends to the outside and the fixedly connected with arm-tie of kit box body.

Preferably, first chutes are formed in two sides of the kit body, first sliding blocks are connected in the two first chutes in a sliding mode, return springs are fixedly connected to the bottoms of the two first sliding blocks, and the bottoms of the two return springs are fixedly connected with the inner walls of the bottoms of the corresponding first chutes respectively.

Preferably, all rotate on two first sliders and be connected with the rotor plate that the slope symmetry set up, the equal fixedly connected with in top of two fixing clip poles rotates the seat, and the one end that two rotor plates kept away from each other rotates with corresponding rotation seat respectively and is connected.

Preferably, the second sliding grooves are formed in the tops of the two fixing clamping rods, the second sliding blocks are fixedly connected to the inner walls of the bottoms of the two fixing holes, and the two second sliding blocks are respectively in sliding connection with the corresponding second sliding grooves.

Preferably, the number of the fixed clamping grooves is multiple, and the fixed clamping grooves are distributed on the sliding plate at equal intervals in the vertical direction.

Compared with the prior art, the utility model provides a T cell CAR molecule expression analysis detect reagent box possesses following beneficial effect:

1. according to the T cell CAR molecule expression analysis detection kit, two pull plates are pulled to one side, away from each other, of the two pull plates, the two pull plates drive two fixing clamping rods to move, two first sliding blocks compress two return springs, the two return springs absorb energy, the two fixing clamping rods move out of two fixing clamping grooves, the two sliding plates are released from being fixed, then the connecting plates are pulled to move, the connecting rods drive a plurality of sealing covers to move, and the sealing covers move out of the kit body;

2. according to the T cell CAR molecule expression analysis detection kit, reagent bottles are placed in a fixed opening and a placing groove, then a connecting plate is pressed, a plurality of sealing covers seal the mouths of the reagent bottles, meanwhile, the connecting plate drives two sliding plates to be clamped into the two sliding grooves, after the reagent bottles are sealed, two pulling plates are loosened, two fixing clamping rods are clamped into two fixing clamping grooves under the spring resilience acting force of two reset springs, the two sliding plates are fixed, and the plurality of reagent bottles are sealed;

and the part that does not relate to among the device all is the same with prior art or can adopt prior art to realize, the utility model discloses a plurality of removal and the location to sealed lid seal up the reagent bottle, prevented to appear rocking the medicament that leads to in the reagent bottle easily and spill in carrying the in-process, give people the condition of bringing the loss and appear, very big having made things convenient for the user to carry, improved the security performance of storing, easy and simple to handle, the better use that has made things convenient for medical staff has satisfied user's needs.

Drawings

Fig. 1 is a schematic view of the structure of a T cell CAR molecule expression analysis detection kit provided by the present invention;

fig. 2 is a schematic structural diagram of part a of a T cell CAR molecule expression analysis detection kit provided by the present invention;

fig. 3 is a schematic structural diagram of part B of a T cell CAR molecule expression analysis detection kit according to the present invention.

In the figure: the kit comprises a kit box body 1, a fixing plate 2, a fixing port 3, a placing groove 4, a reagent bottle 5, a sliding groove 6, a sliding plate 7, a connecting plate 8, a connecting block 9, a connecting rod 10, a sealing cover 11, a fixing hole 12, a fixing clamping rod 13, a fixing clamping groove 14, a pulling plate 15, a first sliding groove 16, a first sliding block 17, a return spring 18, a rotating plate 19, a rotating seat 20, a second sliding groove 21 and a second sliding block 22.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-3, a kit for analyzing and detecting the molecular expression of T cell CAR comprises a kit body 1 with an opening at the top, wherein the inner walls of two sides of the kit body 1 are fixedly connected with a same fixed plate 2, the fixed plate 2 is provided with a plurality of fixed ports 3, the inner wall of the bottom of the kit body 1 is provided with a plurality of placing grooves 4, a same reagent bottle 5 with an opening at the top is placed in the placing groove 4 and the fixed port 3, the top of the kit body 1 is provided with two sliding grooves 6, sliding plates 7 are slidably connected in the two sliding grooves 6, the top ends of the two sliding plates 7 extend to the upper side of the kit body 1 and are fixedly connected with a same connecting plate 8, the bottom of the connecting plate 8 is fixedly connected with two connecting blocks 9, the bottom ends of the two connecting blocks 9 extend to the kit body 1 and are fixedly connected with a same connecting rod 10, a plurality of bottoms of the bottom fixedly connected with of connecting rod 10 are the sealed lid 11 of open-ended, and a plurality of sealed lids 11 respectively with the sealed cooperation of corresponding reagent bottle 5, fixed orifices 12 have all been seted up to the both sides of kit box body 1, and two fixed orifices 12 are linked together with corresponding sliding tray 6 respectively, equal sliding connection has fixing clip pole 13 in two fixed orifices 12.

The utility model discloses in, fixed slot 14 has all been seted up to one side that two sliding plates 7 kept away from each other, and the one end that two fixing clip poles 13 are close to each other clamps with corresponding fixed slot 14 respectively mutually, and the one end that two fixing clip poles 13 kept away from each other all extends to the outside of kit box body 1 and fixedly connected with arm-tie 15.

The utility model discloses in, first spout 16 has all been seted up to the both sides of kit box body 1, and equal sliding connection has first slider 17 in two first spouts 16, and the equal fixedly connected with reset spring 18 in bottom of two first sliders 17, the bottom of two reset spring 18 respectively with the bottom inner wall fixed connection of corresponding first spout 16.

The utility model discloses in, all rotate the rotor plate 19 that is connected with the slope symmetry and sets up on two first sliders 17, the equal fixedly connected with in top of two fixing clip poles 13 rotates seat 20, and the one end that two rotor plates 19 kept away from each other rotates with corresponding rotation seat 20 respectively and is connected.

The utility model discloses in, second spout 21 has all been seted up at the top of two fixing clip poles 13, equal fixedly connected with second slider 22 on the bottom inner wall of two fixed orificess 12, and two second sliders 22 respectively with corresponding second spout 21 sliding connection.

The utility model discloses in, fixed slot 14's quantity is a plurality of, and a plurality of fixed slot 14 are vertical direction equidistance and distribute on sliding plate 7.

In the utility model, when in use, firstly, two pulling plates 15 are pulled to one side where the two pulling plates 15 are far away from each other, the two pulling plates 15 drive two fixing clamping rods 13 to move, the two fixing clamping rods 13 drive two rotating seats 20 to move, the two rotating seats 20 drive two rotating plates 19 to move, the two rotating plates 19 drive two first sliders 17 to move, the two first sliders 17 compress two reset springs 18 to absorb energy through the two reset springs 18, meanwhile, the two second sliders 22 limit the two fixing clamping rods 13, so that the two fixing clamping rods 13 move out of the two fixing clamping grooves 14, the fixing of the two sliding plates 7 is released, then the connecting plate 8 is pulled to move, the connecting plate 8 drives two connecting blocks 9 to move, the two connecting blocks 9 drive the same connecting rod 10 to move, the connecting rod 10 drives a plurality of sealing covers 11 to move, the reagent bottle sealing device has the advantages that a plurality of sealing covers 11 are moved out of a reagent box body 1, reagent bottles 5 are placed in a fixing port 3 and a placing groove 4, a connecting plate 8 is pressed, the connecting plate 8 drives the sealing covers 11 to move downwards, the sealing covers 11 seal the mouths of the reagent bottles 5, meanwhile, the connecting plate 8 drives two sliding plates 7 to be clamped into two sliding grooves 6, after the reagent bottles 5 are sealed, two pull plates 15 are loosened, two fixing clamping rods 13 are clamped into two fixing clamping grooves 14 under the resilience action force of springs of two reset springs 18, the two sliding plates 7 are fixed, the reagent bottles 5 are sealed through the movement and the positioning of the sealing covers 11, the phenomenon that reagents in the reagent bottles 5 are prone to spill due to shaking in the carrying process is avoided, and loss is brought to people is avoided, the portable medical storage box has the advantages that the portable medical storage box is greatly convenient for a user to carry, the storage safety performance is improved, the operation is simple and convenient, the use of medical personnel is facilitated better, and the requirements of the user are met.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A T cell CAR molecule expression analysis detection kit comprises a kit box body (1) with an open top, and is characterized in that the inner walls of two sides of the kit box body (1) are fixedly connected with the same fixed plate (2), the fixed plate (2) is provided with a plurality of fixed ports (3), the inner wall of the bottom of the kit box body (1) is provided with a plurality of placing grooves (4), the same reagent bottle (5) with the open top is placed in the placing groove (4) and the fixed port (3), the top of the kit box body (1) is provided with two sliding grooves (6), sliding plates (7) are slidably connected in the two sliding grooves (6), the top ends of the two sliding plates (7) extend to the upper part of the kit box body (1) and are fixedly connected with the same connecting plate (8), and the bottom of the connecting plate (8) is fixedly connected with two connecting blocks (9), the bottom of two connecting blocks (9) all extends to the same connecting rod of fixedly connected with (10) in kit box body (1), a plurality of bottoms of the bottom fixedly connected with of connecting rod (10) are the sealed lid of open-ended (11), and a plurality of sealed lids (11) respectively with corresponding reagent bottle (5) sealed cooperation, fixed orifices (12) have all been seted up to the both sides of kit box body (1), two fixed orifices (12) are linked together with corresponding sliding tray (6) respectively, equal sliding connection has fixing clip pole (13) in two fixed orifices (12).

2. The kit for analyzing and detecting the expression of the T cell CAR molecule according to claim 1, wherein the two sliding plates (7) are provided with fixing grooves (14) on the sides away from each other, the ends of the two fixing clamping rods (13) close to each other are respectively clamped with the corresponding fixing grooves (14), and the ends of the two fixing clamping rods (13) away from each other extend to the outer side of the kit body (1) and are fixedly connected with pulling plates (15).

3. The kit for analyzing and detecting the expression of the T cell CAR molecule according to claim 1, wherein the kit body (1) is provided with first sliding grooves (16) on both sides thereof, the first sliding blocks (17) are slidably connected in the two first sliding grooves (16), the bottom parts of the two first sliding blocks (17) are fixedly connected with return springs (18), and the bottom ends of the two return springs (18) are fixedly connected with the inner walls of the bottom parts of the corresponding first sliding grooves (16), respectively.

4. The kit for detecting the expression of the T cell CAR molecule according to claim 3, wherein the two first sliders (17) are rotatably connected with rotating plates (19) which are obliquely and symmetrically arranged, the tops of the two fixing clamping rods (13) are fixedly connected with rotating seats (20), and the ends of the two rotating plates (19) which are far away from each other are respectively rotatably connected with the corresponding rotating seats (20).

5. The kit for detecting the expression of the T cell CAR molecule according to claim 1, wherein the top of each of the two fixing clip rods (13) is provided with a second sliding groove (21), the bottom inner walls of the two fixing holes (12) are fixedly connected with a second slider (22), and the two second sliders (22) are respectively connected with the corresponding second sliding grooves (21) in a sliding manner.

6. The kit for detecting the expression of a T cell CAR molecule according to claim 2, wherein the number of the fixed card slots (14) is plural, and the plural fixed card slots (14) are vertically and equidistantly distributed on the sliding plate (7).