CN214991606U

CN214991606U - Phage and probiotic coupling fermentation broth culture device

Info

Publication number: CN214991606U
Application number: CN202120063078.3U
Authority: CN
Inventors: 何四龙; 丛郁; 徐旭凌; 乔欢; 肖逍; 许文建; 丁良; 于浩; 樊小九; 徐天舜; 许小康; 李涛; 华雨田
Original assignee: Figelok China Biotechnology Co ltd
Current assignee: Figelok China Biotechnology Co ltd
Priority date: 2021-01-12
Filing date: 2021-01-12
Publication date: 2021-12-03
Anticipated expiration: 2031-01-12

Abstract

The utility model discloses a phage and benefit fungus coupling zymotic fluid culture apparatus, which comprises a housin, be equipped with the phage incubator in the casing, coupling incubator and control box, the control box links to each other with phage incubator and coupling incubator respectively, the phage incubator sets up the one side at the coupling incubator, the phage incubator is including setting up the phage culture room in inside and setting up the first heating chamber in phage culture room periphery, the coupling incubator is including setting up the coupling culture room in inside and setting up the second heating chamber in coupling culture room periphery, the phage culture room passes through the chimney filter with the coupling culture room and is connected, the phage culture room still is connected with aseptic compressed air jar through the pipeline. The utility model discloses culture, filtration and the coupling of bacteriophage are good for the fungus fermentation culture and are united, reduce other fungus class pollutions in the process of cultivateing as far as possible, the convenient bacteriophage and the coupling zymotic fluid of living fungus that obtains.

Description

Phage and probiotic coupling fermentation broth culture device

Technical Field

The utility model relates to a biological fermentation liquid cultivates the field, concretely relates to phage and probiotic coupling zymotic fluid culture apparatus.

Background

The probiotics is a microorganism which is beneficial to the health of organisms, plays a beneficial role because the probiotics can improve the micro-ecological balance of a host, and is mainly applied to intestinal infectious diseases and chronic inflammatory intestinal diseases clinically. The probiotics enter the intestinal tract of a human body through oral administration, generate specific spread adhesion with epithelial cells of the intestinal tract through biochemical action, and form a biological barrier together with normal flora to protect the mucous membrane of the gastrointestinal tract. Bacteriophages are viruses that attack bacteria and are also genetic material that confers a biological trait to a host bacterium. The bacteriophage must parasitize in live bacteria, has strict host specificity, depends on the molecular structure and complementarity of the bacteriophage on the surface receptors of the organ adsorbed by the bacteriophage and the recipient bacteria, and can effectively inhibit the growth of harmful bacterial cells, eliminate harmful bacteria and further care the growth of probiotics. Only single phage culture apparatus or probiotic culture apparatus among the prior art, when carrying out phage and probiotic coupling culture, easily receive the pollution of other fungus class in the process, the operation process is complicated.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a phage and probiotic coupling zymotic fluid culture apparatus aiming at the defects existing in the prior art.

In order to achieve the above object, the utility model provides a phage and probiotic coupling zymotic fluid culture apparatus, including the casing, be equipped with phage incubator, coupling incubator and control box in the casing, the control box links to each other with phage incubator and coupling incubator respectively, the phage incubator sets up the one side at the coupling incubator, phage incubator and coupling incubator all are back style of calligraphy, the phage incubator is including setting up the phage culture room in inside and setting up in the first heating chamber of phage culture room periphery, the coupling incubator is including setting up the coupling culture room in inside and setting up in the second heating chamber of coupling culture room periphery, the phage culture room passes through the chimney filter with the coupling culture room and is connected, all be equipped with the heat-conducting plate between phage culture room and the first heating chamber and between coupling culture room and the second heating chamber, the casing upside be equipped with the phage cultivate the first inlet of room being connected, with the first water filling port of first heating chamber connection, with the second inlet of room connection is cultivateed in the coupling and with the second water filling port of second heating chamber connection, be equipped with heating element in first heating chamber and the second heating chamber respectively, the phage cultivate the room and still be connected with aseptic compressed air jar through the pipeline, be equipped with the solenoid valve on the pipeline, the solenoid valve is connected with the control box, be equipped with the baroceptor of being connected with the control box in the phage cultivate the room.

Further, be equipped with first agitator in the phage culture room, first agitator links to each other with first motor, be equipped with the second agitator in the coupling culture room, the second agitator links to each other with the second motor, first motor and second motor are connected with the control box respectively.

Further, the heating assembly comprises a first heating resistor arranged at the bottom of the first heating chamber, a first temperature sensor arranged on the first heating chamber, a second heating resistor arranged at the bottom of the second heating chamber and a second temperature sensor arranged on the second heating chamber, and the first heating resistor, the first temperature sensor, the second heating resistor and the second temperature sensor are respectively connected with the control box.

Furthermore, the upper side of the phage culture chamber is provided with a first observation window, and the upper side of the coupling culture chamber is provided with a second observation window.

Further, the phage culture chamber is connected with a first inflation inlet, a first filter is arranged on the first inflation inlet, the coupling culture chamber is connected with a second inflation inlet and a liquid outlet, and a second filter is arranged on the second inflation inlet.

Furthermore, the filter tube comprises a filter membrane, a channel and a one-way valve, wherein the upper end of the filter membrane is connected with a filter control valve, and the filter control valve is connected with the control box.

Furthermore, the lower part of the shell is fixedly connected with a roller.

Has the advantages that: the utility model discloses culture, filtration and the coupling of bacteriophage are good for the fungus fermentation culture and are united, reduce other fungus class pollutions of in-process as far as possible, the convenient bacteriophage and the coupling zymotic fluid that obtains of benefit.

Drawings

FIG. 1 is a schematic structural diagram of a phage and probiotic coupled fermentation broth culture apparatus according to an embodiment of the present invention;

fig. 2 is a schematic diagram of the internal structure of the embodiment of the present invention.

In the figure: 1. a first water injection port; 2. a first temperature sensor; 3. an air pressure sensor; 4. a first stirrer; 5. a first liquid inlet; 6. a second water injection port; 7. a second temperature sensor; 8. a second agitator; 9. a second liquid inlet; 10. a second inflation port; 11. a second filter; 12. a roller; 13. a sterile compressed air tank; 14. an electromagnetic valve; 15. a first heating rod; 16. a first motor; 17. a filtration control valve; 18. a control box; 19. a second heating rod; 20. a one-way valve; 21. a second motor; 22. a liquid outlet; 23. a liquid outlet control valve; 24. a first observation window; 25. a control room door; 26. a second observation window; 27 phage culture chamber; 28. a first heating chamber; 29 a second heating chamber; 30. a coupling culture chamber; 31. a first inflation port; 32. a first filter; 33. filtering the membrane; 34. a channel.

Detailed Description

The present invention will be further clarified by the following embodiments with reference to the attached drawings, which are implemented on the premise of the technical solution of the present invention, and it should be understood that these embodiments are only used for illustrating the present invention and are not used for limiting the scope of the present invention.

As shown in fig. 1 to 2, the embodiment of the present invention provides a phage and probiotic coupling fermentation broth culture apparatus, which comprises a housing, a phage incubator, a coupling incubator and a control box 18 are disposed in the housing, the control box 18 is respectively connected with the phage incubator and the coupling incubator, the phage incubator is disposed on one side of the coupling incubator, the phage incubator and the coupling incubator are both in a shape of a Chinese character hui, the phage incubator comprises a phage culture chamber 27 disposed inside and a first heating chamber 28 disposed at the periphery of the phage culture chamber 27, the coupling incubator comprises a coupling culture chamber 30 disposed inside and a second heating chamber 29 disposed at the periphery of the coupling culture chamber 30, the phage culture chamber 27 is connected with the coupling culture chamber 30 through a filter tube, heat conducting plates are disposed between the phage culture chamber 27 and the first heating chamber 28 and between the coupling culture chamber 30 and the second heating chamber 29, the phage culture chamber 27 and the coupling culture chamber 30 are separated from the first heating chamber 28 and the second heating chamber 29, respectively, by heat conductive plates made of heat conductive material including metal such as copper, aluminum, etc., and both sides of the heat conductive plates are preferably provided with anti-corrosive layers such as electro-galvanized layers. The upper side of the shell is provided with a first liquid inlet 5 connected with a bacteriophage culture chamber 27, a first water filling port 1 connected with a first heating chamber 28, a second liquid inlet 9 connected with a coupling culture chamber 30 and a second water filling port 6 connected with a second heating chamber 29, heating components are respectively arranged in the first heating chamber 28 and the second heating chamber 29, the bacteriophage culture chamber 27 is also connected with a sterile compressed air tank 13 through a pipeline, the sterile compressed air tank 13 is preferably arranged in the shell, an electromagnetic valve 14 is arranged on the pipeline, the electromagnetic valve 14 is connected with a control box 18, and an air pressure sensor 3 connected with the control box 18 is arranged in the bacteriophage culture chamber 27. The lower part of the housing is preferably fixedly connected with rollers 12, on which a control room door 25 is preferably provided for facilitating maintenance of the device and replacement of the sterile compressed air tank 13.

The phage culture chamber 27 is internally provided with a first stirrer 4, the first stirrer 4 is connected with a first motor 16, the coupling culture chamber 30 is internally provided with a second stirrer 8, the second stirrer 8 is connected with a second motor 21, and the first motor 16 and the second motor 21 are respectively connected with a control box 18. The upper side of the phage culture chamber 27 is provided with a first observation window 24, and the upper side of the coupling culture chamber 30 is provided with a second observation window 26. The blades of the first stirrer 4 and the second stirrer 8 are preferably set to be in a staggered inclined shape, the control box 18 can respectively control the first motor 16 and the second motor 21 to operate, the first motor 16 and the second motor 21 respectively drive the first stirrer 4 and the second stirrer 8 to rotate and stir when working, so that the fermentation liquor can be stirred more uniformly, the fermentation liquor can be observed through the first observation window 24 and the second observation window 26, and the observation windows are preferably detachable.

The heating assembly comprises a first heating resistor 15 arranged at the bottom of the first heating chamber 28, a first temperature sensor 2 arranged on the first heating chamber 28, a second heating resistor 19 arranged at the bottom of the second heating chamber 29 and a second temperature sensor 7 arranged on the second heating chamber 29, wherein the first heating resistor 15, the first temperature sensor 2, the second heating resistor 19 and the second temperature sensor 7 are respectively connected with the control box 18.

The phage culture chamber 27 is connected with a first inflation inlet 31, a first filter 32 is arranged on the first inflation inlet 31, the coupling culture chamber is connected with a second inflation inlet 10 and a liquid outlet 22, a liquid outlet control valve 23 connected with the control box 18 is preferably arranged on the liquid outlet 22, and a second filter 11 is arranged on the second inflation inlet 10. During the process of phage culture, if gas such as oxygen needs to be blended in can be filled through the first inflation inlet 31, bacteria and impurities in the gas are filtered by the first filter 32, so that pollution during the culture process is reduced, the second inflation inlet 10 is used when gas such as oxygen needs to be blended in during coupled culture, plugs or covers can be further arranged on the two inflation inlets, and pollution entering from the inflation inlets is reduced when the gas is not used.

The filter tube comprises a filter membrane 33, a channel 34 and a one-way valve 20, preferably the filter membrane 33 is a 0.22 μm filter membrane, the upper end of the filter membrane 33 is connected with a filter control valve 17, and the filter control valve 17 is connected with a control box 18.

Before phage culture, water is injected into the first heating chamber 28 from a water inlet, the first heating resistor 15 heats the water, the first temperature sensor 2 transmits the collected temperature information to the control box 18, the control box controls the heating condition of the first heating resistor 15 according to the set temperature, meanwhile, the heat conducting plate conducts heat, the temperature can be gently transmitted to the phage culture chamber 27 by adopting a water bath heating mode, the materials required by phage culture can be poured from the first liquid inlet 5, stirring is started at the speed required by the culture after the phage culture is ready, culture fermentation is carried out, when the culture of phage fermentation liquid is finished, the control box 18 controls the filtration control valve 17, the one-way valve 20 and the electromagnetic valve 14 to be opened, sterile compressed air enters the phage culture chamber 27 to pressurize the culture chamber to reach the pressure required by the separation of phage and host bacteria through a filter membrane, the air pressure sensor 3 transmits the collected air pressure information to the control box 18, so as to control and maintain the required pressure range, filtering the phage fermentation liquid by a filter membrane, allowing the filtered phage to enter the coupling culture chamber 30 along a channel 34, putting the probiotic seed liquid into the coupling culture chamber 30 for coupling fermentation culture of the two, and taking out the phage and probiotic coupling fermentation liquid from the liquid outlet 22 after the culture is finished.

The above description is only a preferred embodiment of the present invention, and it should be noted that other parts not specifically described belong to the prior art or the common general knowledge to those skilled in the art. Without departing from the principle of the present invention, several improvements and decorations can be made, and these improvements and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The utility model provides a phage and probiotic coupling zymotic fluid culture apparatus, characterized in that, includes the casing, be equipped with phage incubator, coupling incubator and control box (18) in the casing, control box (18) link to each other with phage incubator and coupling incubator respectively, the phage incubator sets up the one side at the coupling incubator, phage incubator and coupling incubator all are back style of calligraphy, the phage incubator is including setting up phage culture room (27) and setting in inside first heating chamber (28) of phage culture room (27) periphery, the coupling incubator is including setting up coupling incubator (30) in inside and setting up second heating chamber (29) of coupling incubator (30) periphery, phage culture room (27) and coupling incubator (30) are through the chimney filter connection, between phage culture room (27) and first heating chamber (28) and coupling incubator (30) and the second heating chamber (29) of coupling incubator (30) periphery All be equipped with the heat-conducting plate between two heating chambers (29), the casing upside be equipped with phage cultivate first inlet (5) of being connected of room (27), first water filling port (1) of being connected with first heating chamber (28), with second inlet (9) that room (30) are cultivated in the coupling and with second water filling port (6) that second heating chamber (29) are connected, be equipped with heating element in first heating chamber (28) and second heating chamber (29) respectively, phage cultivate room (27) and still be connected with aseptic compressed air jar (13) through the pipeline, be equipped with solenoid valve (14) on the pipeline, solenoid valve (14) are connected with control box (18), be equipped with baroceptor (3) of being connected with control box (18) in phage cultivation room (27).

2. The phage and probiotic coupled fermentation broth culture device according to claim 1, characterized in that a first stirrer (4) is arranged in the phage culture chamber (27), the first stirrer (4) is connected with a first motor (16), a second stirrer (8) is arranged in the coupled culture chamber (30), the second stirrer (8) is connected with a second motor (21), and the first motor (16) and the second motor (21) are respectively connected with a control box (18).

3. The phage and probiotic coupled fermentation broth culture device according to claim 1, characterized in that the heating assembly comprises a first heating resistor (15) arranged at the bottom of the first heating chamber (28), a first temperature sensor (2) arranged on the first heating chamber (28), a second heating resistor (19) arranged at the bottom of the second heating chamber (29) and a second temperature sensor (7) arranged on the second heating chamber (29), and the first heating resistor (15), the first temperature sensor (2), the second heating resistor (19) and the second temperature sensor (7) are respectively connected with the control box (18).

4. The device for culturing the phage and probiotic coupled fermentation broth according to claim 1, wherein the phage culture chamber (27) is provided with a first observation window (24) on the upper side, and the coupled culture chamber (30) is provided with a second observation window (26) on the upper side.

5. The device for culturing the phage and probiotic coupled fermentation broth according to claim 1, wherein the phage culture chamber (27) is connected with a first inflation port (31), a first filter (32) is arranged on the first inflation port (31), the coupled culture chamber is connected with a second inflation port (10) and a liquid outlet (22), and a second filter (11) is arranged on the second inflation port (10).

6. The phage and probiotic coupled fermentation broth culture device according to claim 1, characterized in that the filter tube comprises a filter membrane (33), a channel (34) and a one-way valve (20), the upper end of the filter membrane (33) is connected with a filtration control valve (17), and the filtration control valve (17) is connected with a control box (18).

7. The phage and probiotic coupled fermentation broth culture device of claim 1, wherein the lower portion of the housing is fixedly connected with rollers (12).