CN214894829U - Micro-testing capillary device and spectrophotometer - Google Patents
Micro-testing capillary device and spectrophotometer Download PDFInfo
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- CN214894829U CN214894829U CN202120294141.4U CN202120294141U CN214894829U CN 214894829 U CN214894829 U CN 214894829U CN 202120294141 U CN202120294141 U CN 202120294141U CN 214894829 U CN214894829 U CN 214894829U
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- spectrophotometer
- test
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- 238000012360 testing method Methods 0.000 title claims abstract description 64
- 239000010453 quartz Substances 0.000 claims description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 6
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- 238000004891 communication Methods 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 5
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- 230000035945 sensitivity Effects 0.000 description 6
- 230000008901 benefit Effects 0.000 description 3
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- 230000000694 effects Effects 0.000 description 3
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- 238000002798 spectrophotometry method Methods 0.000 description 3
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
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- 239000000463 material Substances 0.000 description 2
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- 210000002421 cell wall Anatomy 0.000 description 1
- NJDNXYGOVLYJHP-UHFFFAOYSA-L disodium;2-(3-oxido-6-oxoxanthen-9-yl)benzoate Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=CC(=O)C=C2OC2=CC([O-])=CC=C21 NJDNXYGOVLYJHP-UHFFFAOYSA-L 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
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Abstract
The embodiment of the utility model provides a micro-testing capillary device and a spectrophotometer, wherein the spectrophotometer comprises a cuvette frame, and the cuvette frame is provided with a placing groove for placing a cuvette; the micro-test capillary device comprising: a fixing portion that can be placed on the cell holder; the capillary tube is detachably arranged on the fixing part, at least one end of the capillary tube extends out of the fixing part, so that when the fixing part is placed on the cuvette frame, the part of the capillary tube extending out of the fixing part extends into the middle of the placing groove. The utility model discloses a trace test capillary device simple structure is small and exquisite, easily assembles, and the cost of manufacture is low, can be applied to the spectrophotometer on, is applicable to the detection of trace and trace sample, and detectivity is high.
Description
Technical Field
The utility model belongs to the technical field of the spectrophotometer measurement, especially, relate to a micro test capillary device and have spectrophotometer of this micro test capillary device.
Background
Spectrophotometry (including fluorescence spectrophotometry and ultraviolet-visible absorption spectrophotometry) is widely applied to the research fields of biochemistry, biomedicine, environmental chemistry, food detection and the like due to the advantages of high sensitivity, good selectivity, simple operation and the like. The spectrophotometry requires a cuvette as an equipment vessel and utilizes a spectrophotometer to detect the object to be detected. Be equipped with the standing groove on the spectrophotometer, during the detection, will hold the cell that detects the sample and place on the standing groove and go on. The cuvette includes a common cuvette, a semimicro cuvette, a micro cuvette, etc. When fewer objects to be detected are detected, a micro cuvette is often used. When a micro cuvette is used for detection, the detection sensitivity is reduced because light needs to penetrate through the thicker quartz wall; in addition, the opening of the micro cuvette is small, and the overflow is easy to occur in the operation process; the micro cuvette has higher manufacturing cost and is not suitable for detecting a trace sample.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned problem that prior art exists, the utility model provides an object of the embodiment provides a trace test capillary device, and it can be applied to the spectrophotometer, is applicable to the detection of trace and trace sample, and detectivity is high.
The embodiment of the utility model provides a technical scheme who adopts is, a micro test capillary device for spectrophotometer, spectrophotometer includes the cell frame, the cell frame has the standing groove that is used for placeeing the cell, its characterized in that, micro test capillary device includes:
a fixing portion that can be placed on the cell holder;
the capillary tube is detachably arranged on the fixing part, at least one end of the capillary tube extends out of the fixing part, so that when the fixing part is placed on the cuvette frame, the part of the capillary tube extending out of the fixing part extends into the middle of the placing groove.
In an optional embodiment, the micro-testing capillary device further comprises a protection part, wherein the protection part is detachably connected with the fixing part and used for covering the part of the capillary outside the fixing part.
In an optional embodiment, the protection part is in a tubular shape with at least one open end, and the protection part is detachably connected with the fixing part through the open end.
In an optional embodiment, the open end of the protection part is connected with one end of the fixing part through threads or clamped through a concave-convex structure.
In an optional embodiment, a through cavity which penetrates through two ends of the fixing part is formed in the fixing part, a rubber ring is fixed in the through cavity, the aperture of the rubber ring is smaller than the outer diameter of the capillary tube, the capillary tube is inserted into the rubber ring, and the inner wall of the rubber ring is tightly attached to the outer wall of the capillary tube, so that the capillary tube is fixed to the fixing part through the rubber ring.
In an alternative embodiment, the capillary is a quartz capillary.
In an optional embodiment, the quartz capillary tube has an inner diameter of 0.5 mm to 0.7 mm, a tube wall thickness of 0.1 mm to 0.3 mm, and a length of 3 cm to 10 cm.
In an optional embodiment, the fixing portion includes a first portion and a second portion that are connected, the size of the first portion is larger than that of the second portion, so that when the fixing portion is disposed on the cell holder, the second portion can extend into the upper end of the placing groove, and the first portion is located outside the placing groove and is mounted on the cell holder.
In an alternative embodiment, the fixing portion is made of plastic; the protection part is made of plastic or glass.
The embodiment of the utility model provides a spectrophotometer simultaneously, it includes the cell frame, the cell frame has the standing groove, a serial communication port, spectrophotometer still includes the micro-test capillary device of any above-mentioned embodiment, micro-test capillary device can arrange in on the cell frame, just micro-test capillary device the capillary stretch into extremely the centre of standing groove.
Compared with the prior art, the utility model discloses micro-test capillary device and spectrophotometer's beneficial effect lies in:
1. the micro-testing capillary device has simple and small structure, easy assembly and low manufacturing cost;
2. the test of the liquid to be tested with different volumes is realized by replacing the capillary tubes with different inner diameters, and the volume of the liquid to be tested is reduced as much as possible;
3. the capillary tube is convenient to absorb sample liquid, and is beneficial to recycling the sample liquid, so that the problem that a cuvette cannot be used due to trace sample liquid is avoided, and the problem that the detection sensitivity is reduced due to the wall thickness of the cuvette is also avoided;
4. the micro-testing capillary device is suitable for any type of fluorescence spectrophotometer or ultraviolet-visible spectrophotometer, and has wide application range.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention.
The summary of various implementations or examples of the technology described in this disclosure is not a comprehensive disclosure of the full scope or all features of the disclosed technology.
Drawings
In the drawings, which are not necessarily drawn to scale, like reference numerals may describe similar components in different views. Like reference numerals having letter suffixes or different letter suffixes may represent different instances of similar components. The drawings illustrate various embodiments, by way of example and not by way of limitation, and together with the description and claims, serve to explain the embodiments of the invention. The same reference numbers will be used throughout the drawings to refer to the same or like parts, where appropriate. Such embodiments are illustrative, and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.
Fig. 1 is a schematic perspective view of a micro-testing capillary device according to an embodiment of the present invention.
Fig. 2 is a front view of fig. 1.
Fig. 3 is a cross-sectional view of fig. 2.
Fig. 4 is a schematic perspective view of the protection part of fig. 1 with the protection part removed.
Fig. 5 is a cross-sectional view of another configuration of a micro-metering test capillary device according to an embodiment of the present invention.
Fig. 6 is a comparison graph of the testing sensitivity of the micro-testing capillary device and the micro cuvette according to the embodiment of the present invention.
Reference numerals:
1-a stationary part; 11-a first part; 12-a second part; 13-a cap; 14-a through cavity; 15-rubber ring; 16-a filler block; 2-a capillary tube; 3-a protection part.
Detailed Description
In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined below to clearly and completely describe the technical solution of the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. All other embodiments, which can be obtained by a person skilled in the art without any inventive work based on the described embodiments of the present invention, belong to the protection scope of the present invention.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by those of ordinary skill in the art to which the invention belongs. The use of "first," "second," and similar terms in the description herein do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
To maintain the following description of the embodiments of the present invention clear and concise, detailed descriptions of well-known functions and components may be omitted.
As shown in fig. 1-5, the embodiment of the utility model provides a micro-test capillary device for on the spectrophotometer, spectrophotometer includes the cell frame, and the cell frame has the standing groove that is used for placeeing the cell. The micro-testing capillary device comprises a fixing part 1 and a capillary tube 2, wherein the fixing part 1 can be placed on a cuvette holder. The capillary 2 is detachably arranged on the fixing part 1, and at least one end of the capillary 2 extends out of the fixing part 1, so that when the fixing part 1 is placed on the cuvette holder, the part of the capillary 2 extending out of the fixing part 1 extends into the middle of the placing groove.
The utility model discloses trace test capillary device can be used for the spectrophotometer on, replaces the cell to use to solve trace and trace sample and utilize the cell to be difficult to detect, and the low problem of detectivity. Adopt the utility model discloses a trace test capillary device only needs the liquid of awaiting measuring of minim just can realize fluorescence ultraviolet visible absorption light detection, because 2 walls of capillary are thinner, greatly reduced is because of the problem of the sensitivity reduction that the wall thickness of micro cell leads to.
In some embodiments, as shown in fig. 1 to 3, the micro-testing capillary device further includes a protection part 3, wherein the protection part 3 is detachably connected to the fixing part 1 and is used for covering a portion of the capillary 2 outside the fixing part 1. The protection part 3 is used for protecting the capillary 2 from being influenced by external factors and avoiding the damage of the capillary 2, thereby prolonging the service life of the capillary 2. When the micro-testing capillary device is used, the protection part 3 is detached from the fixing part 1, so that the capillary 2 is exposed and can extend into the middle of the placing groove, and the detection of a sample in the capillary 2 is facilitated. When the micro-testing capillary device is not used, the protection part 3 is connected to the fixing part 1, and the capillary 2 is coated by the protection part 3, so that the capillary 2 can be effectively protected from being damaged.
The configuration of the protective portion 3 is not limited as long as the capillary 2 can be protected from the outside. As shown in fig. 1 to 3, the protection part 3 of the present embodiment is a tube with at least one open end, and the protection part 3 is detachably connected to the fixing part 1 through the open end. Thus, the capillary tube 2 can be completely enclosed in the protective part 3, and impurities such as dust can be prevented from entering the capillary tube 2, and the tubular protective part 3 can be manufactured and processed conveniently. The cross section of the tubular protection portion 3 may be circular, elliptical, polygonal, etc., and this embodiment is not limited thereto.
In some embodiments, as shown in fig. 4 and 5, the open end of the protection part 3 is engaged with one end of the fixing part 1 by a screw connection or a concave-convex structure. Specifically, continuing to combine fig. 4 and 5, the outer wall of the upper end of the protection portion 3 of this embodiment is provided with an external thread, the inner wall of the lower portion of the fixing portion 1 is provided with an internal thread matched with the external thread, and the protection portion 3 and the fixing portion 1 are detachably connected through a thread. Of course, the protection part 3 may be provided with female threads and the fixing part 1 may be provided with male threads, and the protection part 3 and the fixing part 1 may be screwed together in the same manner. Similarly, the concave-convex structure may be disposed between the inner wall of the protection portion 3 and the outer wall of the fixing portion 1, or between the outer wall of the protection portion 3 and the inner wall of the fixing portion 1, as long as the detachable connection between the two can be realized, and this embodiment does not specifically limit this. The threaded connection and the concave-convex structure connection mode are simple and reliable, and the protection part 3 can be conveniently mounted and dismounted on the fixing part 1.
In some embodiments, as shown in fig. 4 and 5, the fixing portion 1 has a through cavity 14 penetrating through both ends thereof, a rubber ring 15 is fixed in the through cavity 14, and the aperture of the rubber ring 15 is smaller than the outer diameter of the capillary tube 2, so that when the capillary tube 2 is inserted into the rubber ring 15, the rubber ring 15 deforms, the inner wall of the rubber ring 15 is tightly attached to the outer wall of the capillary tube 2, the capillary tube 2 is stably fixed to the fixing portion 1 through the rubber ring 15, and the capillary tube 2 can be kept in a vertical state when extending into the placement groove.
The diameter of the through cavity 14 and the outer diameter of the rubber ring 15 are not limited, and when the diameter of the through cavity 14 is large (for example, the diameter of the through cavity 14 is a positive integer of 5 of the outer diameter of the capillary tube 2), that is, the fixing portion 1 is a similar tubular structure with a thin wall, in order to match with the through cavity 14, the rubber ring 15 may be designed to be large, so that the outer wall of the rubber ring 15 is tightly connected with the cavity wall of the through cavity 14. Under the condition that the diameter of the through cavity 14 is larger, a filling block 16 can be arranged in the through cavity 14, the filling block 16 is fixedly connected with the wall of the through cavity 14, a through hole is formed in the filling block 16 (for example, the aperture of the through hole can be 1-3 times of the outer diameter of the capillary tube 2), and the rubber ring 15 is arranged in the through hole, so that the rubber ring 15 can be designed to be smaller, and the rubber ring 15 is prevented from being arranged too much to influence the use effect.
As shown in fig. 1 to 5, the fixed part 1 shown in this embodiment includes the first portion 11 and the second portion 12 that are connected, and the size of the first portion 11 is greater than the size of the second portion 12, so as to work as the utility model discloses a micro-test capillary device is applied to spectrophotometer, when locating on the cell frame, the less second portion 12 of size can stretch into to the upper end of standing groove in, and the bigger first portion 11 of size then is located outside the standing groove to erect on the cell frame, thereby make the stable cell frame of arranging in of fixed part 1. Simple structure and good use effect.
The specific structural form of the first part 11 and the second part 12 of the fixing part 1 is not limited, and those skilled in the art can select and determine the specific structural form according to actual needs. For convenience of manufacture, the first portion 11 and the second portion 12 are both cylindrical, and the outer diameter of the cylindrical first portion 11 is larger than that of the cylindrical second portion 12.
As shown in fig. 4 and 5, a filling block 16 is disposed in the lower portion of the through cavity 14 of the present embodiment, a through hole is disposed in the filling block 16, the rubber ring 15 is disposed in the through hole, the outer wall of the rubber ring 15 is tightly attached to the wall of the through hole, the upper end of the capillary tube 2 penetrates through the rubber ring 15 and extends into the through cavity 14, and the through cavity 14 is communicated with the outside, so that the pressures at the upper end and the lower end of the capillary tube 2 are balanced, and the liquid to be measured is absorbed by using the capillary phenomenon.
In some embodiments, as shown in fig. 1 to 5, the fixation portion 1 further comprises a cap portion 13 connected to the first portion 11, the cap portion 13 and the second portion 12 being located on opposite sides of the first portion 11, respectively. When the length of the fixing portion 1 in the axial direction of the capillary 2 is short and the length of the capillary 2 protruding upward beyond the rubber ring 15 is long (fig. 4 of the present embodiment is merely an example, and the upper end of the capillary 2 does not extend beyond the fixing portion 1), so that the upper end of the capillary 2 protrudes beyond the fixing portion 1, the upper end may be protected by the cap portion 13. The cap 13 is provided with a through hole communicating with the outside so that the pressure in the through cavity 14 is equal to the atmospheric pressure in the use environment. The cap portion 13 plays a role of protecting the capillary 2, and is convenient for an operator to hold and operate the micro-scale testing capillary device. When the length of the fixing portion 1 in the axial direction of the capillary 2 is sufficient so that the upper end of the capillary 2 can be protected by the fixing portion 1, the cap portion 13 may not be provided, see fig. 5.
The material of the fixing portion 1 and the protecting portion 3 is not limited, and can be selected according to actual needs. For example, the fixing portion 1 may be made of plastic, and the protecting portion 3 may be made of ordinary glass or plastic. The utility model discloses trace detects quartz capillary device is by low in cost's rubber, ordinary glass as the material, obviously reduces the cost of manufacture. This trace detects quartzy capillary device is small and exquisite, easily assembles, and the test of different liquid that awaits measuring is realized to the capillary 2 of the different internal diameters of accessible replacement, and the required volume of liquid that awaits measuring of minimize conveniently absorbs the sample liquid to do benefit to and retrieve the sample liquid, can be applicable to arbitrary model fluorescence spectrophotometer or ultraviolet visible spectrophotometer. This trace detects quartz capillary device has avoided because the unable problem that uses the cell of appearance liquid trace, has also avoided the problem that trace cell wall thickness leads to detectivity to reduce.
The capillary tube 2 is a quartz capillary tube 2; the inner diameter of the quartz capillary tube 2 is 0.5 mm-0.7 mm, the wall thickness of the tube is 0.1 mm-0.3 mm, and the length is 3-10 cm.
The following explains the usage of the micro-testing capillary device of the embodiment of the present invention: 1. firstly, the protection part 3 is detached from the fixing part 1; 2. the capillary tube 2 absorbs the liquid to be detected by utilizing the capillary phenomenon, and the outer wall of the capillary tube 2 is wiped dry by using a piece of lens wiping paper; 3. placing the micro-testing capillary device on a cuvette frame of a spectrophotometer to enable the capillary 2 to be in the middle of the placing groove and enable the capillary 2 to be in a vertical state; 4. starting a spectrophotometer to detect, taking away the micro-testing capillary device from the cuvette frame after the detection is finished, and blowing out the liquid to be detected sucked in the capillary 2 by using an ear washing ball; 5. the capillary tube 2 sucks cleaning solution (ethanol) for cleaning, and then the cleaning solution is blown out by the ear washing ball; 6. the protection part 3 is attached to the fixing part 1, and the capillary 2 is covered and stored.
The embodiment of the utility model provides a spectrophotometer is provided simultaneously, it includes the standing groove, still includes the micro-test capillary device of any above-mentioned embodiment, on the cell frame can be arranged in to the micro-test capillary device, and micro-test capillary device's capillary 2 stretches into to the centre of standing groove to detect the sample in capillary 2.
The embodiment tests the effect, will the utility model discloses a trace test capillary device contrasts with the detectivity of commercially available trace fluorescence test cell. Test results referring to fig. 6, wherein the dotted line represents the test results of the fluorescent micro test capillary device using the capillary having an inner diameter of 0.7 mm, the dotted line represents the test results of the fluorescent micro test capillary device using the capillary having an inner diameter of 0.5 mm, and the solid black line represents the test results of the commercially available micro fluorescent test cuvette. The two devices are used for testing 5 micromolar sodium fluorescein to-be-tested solution under the same testing parameters, the capillary tube is used for testing the solution, the sensitivity is higher, and the commercially available micro-fluorescence cuvette can hardly detect a fluorescence signal.
The above description is intended to be illustrative, and not restrictive, and variations, modifications, substitutions, and alterations may be made to the above-described embodiments by those of ordinary skill in the art within the scope of the present disclosure. Also, the above-described examples (or one or more versions thereof) may be used in combination with each other, and it is contemplated that the embodiments may be combined with each other in various combinations or permutations. The scope of the invention should be determined with reference to the appended claims, along with the full scope of equivalents to which such claims are entitled.
Claims (10)
1. The utility model provides a micro-test capillary device for spectrophotometer, spectrophotometer includes the cell frame, the cell frame has the standing groove that is used for placeeing the cell, its characterized in that, micro-test capillary device includes:
a fixing portion that can be placed on the cell holder;
the capillary tube is detachably arranged on the fixing part, at least one end of the capillary tube extends out of the fixing part, so that when the fixing part is placed on the cuvette frame, the part of the capillary tube extending out of the fixing part extends into the middle of the placing groove.
2. The micro test capillary device according to claim 1, further comprising a protection part detachably connected to the fixing part for covering a portion of the capillary outside the fixing part.
3. The capillary device for micro-testing according to claim 2, wherein the protecting portion is in a tubular shape with at least one end open, and the protecting portion is detachably connected to the fixing portion through the open end.
4. A micro-testing capillary device according to claim 3, wherein the open end of the protecting portion is engaged with one end of the fixing portion by a screw or by a concave-convex structure.
5. The capillary device for micro-testing according to claim 1, wherein the fixing portion has a through cavity therein, the through cavity having a rubber ring fixed therein, the diameter of the rubber ring is smaller than the outer diameter of the capillary, the capillary is inserted into the rubber ring, the inner wall of the rubber ring is tightly attached to the outer wall of the capillary, so that the capillary is fixed to the fixing portion through the rubber ring.
6. The micro test capillary device of claim 1, wherein the capillary is a quartz capillary.
7. The capillary device for micro-testing according to claim 6, wherein the quartz capillary has an inner diameter of 0.5 mm to 0.7 mm, a tube wall thickness of 0.1 mm to 0.3 mm, and a length of 3 cm to 10 cm.
8. The micro-testing capillary device according to claim 2, wherein the fixing portion comprises a first portion and a second portion that are connected, the first portion being larger in size than the second portion so that when the fixing portion is placed on the cuvette holder, the second portion can extend into the upper end of the holding groove, and the first portion is located outside the holding groove and is mounted on the cuvette holder.
9. The micro test capillary device of claim 2, wherein the holder is made of plastic; the protection part is made of plastic or glass.
10. The utility model provides a spectrophotometer, includes the cell frame, the cell frame has the standing groove, a serial communication port, spectrophotometer still includes any one of claims 1 to 9 the trace test capillary device, the trace test capillary device can be arranged in on the cell frame, just the capillary of trace test capillary device stretch into extremely the centre of standing groove.
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Cited By (1)
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CN114264606A (en) * | 2021-12-24 | 2022-04-01 | 复旦大学 | Ultramicro cuvette |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114264606A (en) * | 2021-12-24 | 2022-04-01 | 复旦大学 | Ultramicro cuvette |
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