CN214472560U

CN214472560U - Liquid drop identification system

Info

Publication number: CN214472560U
Application number: CN202023075396.6U
Authority: CN
Inventors: 王立言; 段保峰; 郭肖杰
Original assignee: Wuxi Tmaxtree Biotechnology Co ltd
Current assignee: Wuxi Tmaxtree Biotechnology Co ltd
Priority date: 2020-12-18
Filing date: 2020-12-18
Publication date: 2021-10-22
Anticipated expiration: 2030-12-18

Abstract

The liquid drop identification system comprises a microfluidic chip, a laser emitter and a laser signal receiver, wherein the microfluidic chip comprises a substrate, a pipeline and a liquid drop identification window, the pipeline and the liquid drop identification window are formed in the substrate, the laser emitter is located above the liquid drop identification window, and the laser signal receiver is located below the liquid drop identification window. The utility model discloses can discern the liquid drop fast, simple structure, the operation is stable, easily builds and maintains.

Description

Liquid drop identification system

Technical Field

The utility model belongs to the micro-fluidic field, in particular to liquid drop identification system, particularly the utility model discloses a liquid drop identification system is a system to little liquid drop discernment in the biochip.

Background

The liquid drop microfluidics is an important branch in the research of microfluidic chips, is developed on the basis of the traditional continuous flow microfluidic system in recent years, and is a discontinuous flow microfluidic technology for experimental operation by using dispersed micro-liquid drops generated by two liquid phases which are not mutually soluble. The micro-fluidic liquid drop has small volume, precise control and large real-time monitoring difficulty, and limits the application and popularization of the micro-fluidic liquid drop in the microbial technology.

In droplet microfluidics, the rapid and accurate identification of droplets is a prerequisite for accurate operational identification of droplets. At present, a camera is used for collecting images of a chip, and an edge recognition algorithm is used for realizing accurate recognition of micro-droplets in the chip, but the method needs light source illumination, can seriously interfere spectral signal detection, needs expensive equipment and is not beneficial to application of droplet microfluidics in microorganism experiment operation. The light-emitting layer is controlled to emit infrared light with a set wavelength, and the liquid drop received by the photosensitive layer is obtained to identify the liquid drop due to the current generated by the infrared light.

Disclosure of Invention

The present inventors have conducted intensive studies in order to overcome the above problems. Particularly, the utility model provides a little liquid drop identification system.

The utility model adopts the technical scheme as follows:

a liquid drop identification system utilizes a laser transmitter and a laser signal receiver to identify liquid drops of a liquid drop identification window of a microfluidic chip, laser generated by the laser transmitter obliquely enters the liquid drop identification window, an optical signal of the laser signal receiver is converted into an electric signal to be output, and liquid drop identification is carried out according to different signal intensities.

Preferably, the utility model discloses liquid drop identification system includes micro-fluidic chip, laser emitter, laser signal acceptor, micro-fluidic chip includes the base plate, forms pipeline and liquid drop discernment window in the base plate, laser emitter is located liquid drop discernment window top, the laser signal acceptor is located drop discernment window below.

Preferably, the laser emitter is located 0.5-15cm above the droplet identification window, the laser signal receiver is located 0-0.5cm directly below the droplet identification window, and the droplet identification window is a transparent area on the pipeline.

Preferably, the thickness of the microfluidic chip is 3-7mm, the cross section of the pipeline is circular or square, and the range of the cross section area is 0.04-4mm²。

Preferably, the microfluidic chip is formed of glass, Polymethylmethacrylate (PMMA), Polycarbonate (PC), Polystyrene (PS), acrylonitrile-butadiene-styrene copolymer (ABS).

Preferably, the laser emitter is a semiconductor laser, the laser signal receiver is a photoelectric sensor, the semiconductor laser is located 6-10cm above the droplet identification window, and the photoelectric sensor is attached to the droplet identification detection window.

Preferably, the semiconductor laser emits laser with any wavelength of 480nm, 520nm, 560nm, 590nm, 620nm, 650nm and 670nm, and the area of a light spot generated by the laser covers the width of the micro-fluidic chip pipeline. Further preferably, the laser generates a spot area covering the width of the droplet in the microfluidic chip.

Preferably, the laser light excited by the semiconductor laser enters the droplet identification window at an incident angle of 20-70 degrees, and the difference between the optical voltage signal values of the oil phase and the water phase in the microfluidic chip is more than 0.5V.

Preferably, the incident angle of the laser light on the droplet recognition window is 25 to 60 degrees, and further preferably 36 degrees.

Preferably, the droplet size is 0.6-10 μ L.

Preferably, each droplet recognition time is less than 0.5 s.

The utility model discloses a laser emitter produces laser to with certain angle oblique incidence to the liquid drop identification window of chip passageway, photoelectric sensing ware receives the light signal in liquid drop identification window below, because oil phase and aqueous phase refracting index are different, causes the scattering and the refraction of light to have great difference, consequently gets into the light signal intensity of photoelectric sensing ware and the signal of telecommunication of conversion output have obvious difference, and then quick accurate identification oil phase and aqueous phase. The utility model discloses simple structure, the operation is stable, easily builds and maintains.

Drawings

Fig. 1 is a schematic structural view of a droplet identification system of the present invention;

FIG. 2 is a schematic diagram showing a partial enlargement of a droplet recognition system according to the present invention, which is perpendicular to the direction of the droplet flow;

FIG. 3 is a diagram of the detection result of the embodiment of the droplet identification system of the present invention;

the device comprises a micro-fluidic chip 1, a laser emitter 2, a laser signal receiver 3, a substrate 4, a pipeline 5, a droplet identification window 6 and a droplet 7.

Detailed Description

Specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While specific embodiments of the invention are shown in the drawings, it will be understood that the invention may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

It should be noted that certain terms are used throughout the description and claims to refer to particular components. As one skilled in the art will appreciate, various names may be used to refer to a component. This specification and claims do not intend to distinguish between components that differ in name but not function. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. When an element is referred to as being "disposed on" or "connected" to another element, it can be directly connected or indirectly connected to the other element, and the following description is of the best mode for carrying out the invention and is made for the purpose of illustrating the general principles of the description and not for the purpose of limiting the invention. The protection scope of the present invention is subject to the limitations defined by the appended claims.

For the purpose of facilitating understanding of the embodiments of the present invention, the following description will be given by way of example with reference to the accompanying drawings, and the drawings do not limit the embodiments of the present invention.

As shown in fig. 1, a droplet identification system includes a microfluidic chip 1, a laser emitter 2, and a laser signal receiver 3, where the microfluidic chip includes a substrate 4, a channel 5 formed in the substrate, and a droplet identification window 6, and the channel 6 contains a droplet 7.

In one embodiment, the thickness of the microfluidic chip 1 is 3-7mm, the cross section of the pipeline is circular or square, and the range of the cross section area is 0.04-4mm²Preferably 0.64 to 3.24 mm²More preferably 1 to 2.25 mm²More preferably 1 mm². The cross-section of the pipe in one embodiment is as shown in figure 2.

In the utility model, the micro-fluidic chip 1 is formed by glass, polymethyl methacrylate (PMMA), Polycarbonate (PC), Polystyrene (PS), acrylonitrile-butadiene-styrene copolymer (ABS). The droplet recognition window is a transparent area on the pipeline.

In one embodiment, the laser emitter is located above the drop recognition window and the laser signal receiver is located below the drop recognition window. The laser emitter can be placed at any position above the droplet identification window, and the laser signal receiver can be placed at any position below the droplet identification window, as long as the laser energy emitted by the laser emitter is incident on the droplet identification window and can be captured by the laser signal receiver.

In order to save space, the laser emitter is located 0.5-15cm above the droplet identification window, and the laser signal receiver is located 0-0.5cm directly below the droplet identification window.

In one embodiment, the laser emitter is a semiconductor laser, the laser signal receiver is a photoelectric sensor, the semiconductor laser is located 6-10cm above the droplet identification window, and the distance is selected in consideration of convenience in chip mounting and dismounting and in consideration of laser focusing conditions of the selected semiconductor laser; the photoelectric sensor is tightly attached to the drop identification detection window.

The semiconductor laser emits laser with any wavelength of 480nm, 520nm, 560nm, 590nm, 620nm, 650nm and 670nm, and the area of a light spot generated by the laser covers the width of a micro-fluidic chip pipeline. Further, the effect of detection and identification can be achieved by covering the width of the liquid drop in the microfluidic chip by the area of the light spot generated by the laser.

In the utility model, the different refractivities of oil phase and water phase are considered, and the laser obliquely shoots into the liquid drop identification window 6 at a certain angle. In one embodiment, the laser light excited by the semiconductor laser enters the droplet identification window at an incident angle of 20-70 degrees, the incident angle is an included angle between the droplet identification window and the horizontal direction, as shown in fig. 2, the difference between the optical voltage signal values of the oil phase and the water phase in the microfluidic chip is more than 0.5V.

In order to further improve the difference value of the optical voltage signals of the oil phase and the water phase in the microfluidic chip, the incident angle of the laser on the droplet identification window is 25-60 degrees.

In the utility model, the identification time of a single liquid drop is less than 0.5 s.

In a specific embodiment, the size of the droplet in the microfluidic chip pipeline is 0.6-10 μ L, further preferably 0.7-7 μ L, further preferably 0.8-6 μ L, further preferably 1-3 μ L, further preferably 2 μ L. The laser incident angle is 36 degrees, and the difference value of the optical voltage signals of the oil phase and the water phase in the micro-fluidic chip induced by the photoelectric sensor reaches more than 2.4V.

The utility model discloses utilize the scattering and the refraction principle of light, produce laser through laser emitter and jet into liquid drop discernment window with certain angle to one side, because oil phase and aqueous phase refracting index are different, the light signal intensity who gets into the photoelectric sensor has obvious difference with the signal of telecommunication of conversion output, and then quick accurate discernment liquid drop. The utility model discloses simple structure, the operation is stable, easily builds and maintains.

Claims

1. The liquid drop identification system is characterized by comprising a micro-fluidic chip, a laser emitter and a laser signal receiver, wherein the micro-fluidic chip comprises a substrate, a pipeline and a liquid drop identification window, the pipeline and the liquid drop identification window are formed in the substrate, the laser emitter is located 0.5-15cm above the liquid drop identification window, the laser signal receiver is located 0-0.5cm below the liquid drop identification window, and the liquid drop identification window is a transparent area on the pipeline.

2. A droplet identification system according to claim 1, wherein the microfluidic chip is adapted to be mounted on a substrateThe thickness is 3-7mm, the cross section of the pipeline is round or square, and the range of the cross section area is 0.04-4mm²。

3. A droplet identification system according to claim 1, wherein the laser emitter is a semiconductor laser and the laser signal receiver is a photosensor, the semiconductor laser being located 6-10cm above the droplet identification window, the photosensor being located proximate to the droplet identification detection window.

4. A droplet identification system according to claim 3, wherein said semiconductor laser emits laser light of any one of 480nm, 520nm, 560nm, 590nm, 620nm, 650nm, 670 nm.

5. The droplet identification system of claim 4, wherein the semiconductor laser generates a laser spot area covering the width of the microfluidic chip channel.

6. The droplet identification system of claim 5, wherein the laser light excited by the semiconductor laser enters the droplet identification window at an incident angle of 20-70 degrees, and the difference between the optical voltage signal values of the oil phase and the water phase in the microfluidic chip is more than 0.5V.

7. A droplet identification system according to claim 6, wherein the angle of incidence of the laser light on the droplet identification window is 25-60 degrees.

8. A droplet identification system according to claim 7, wherein the droplet size is 0.6-10 μ L and each droplet identification time is less than 0.5 s.