CN214193205U - Freezing and thawing box for thawing cryopreserved umbilical cord hematopoietic stem cells - Google Patents
Freezing and thawing box for thawing cryopreserved umbilical cord hematopoietic stem cells Download PDFInfo
- Publication number
- CN214193205U CN214193205U CN202120046701.4U CN202120046701U CN214193205U CN 214193205 U CN214193205 U CN 214193205U CN 202120046701 U CN202120046701 U CN 202120046701U CN 214193205 U CN214193205 U CN 214193205U
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- Prior art keywords
- box body
- pipe
- freeze
- freezing
- thawing
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- 238000010257 thawing Methods 0.000 title claims abstract description 31
- 210000003958 hematopoietic stem cell Anatomy 0.000 title claims abstract description 21
- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 14
- 230000008014 freezing Effects 0.000 title abstract description 40
- 238000007710 freezing Methods 0.000 title abstract description 40
- 238000005138 cryopreservation Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 30
- 210000004027 cell Anatomy 0.000 abstract description 10
- 230000008018 melting Effects 0.000 abstract description 10
- 238000002844 melting Methods 0.000 abstract description 10
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000000151 deposition Methods 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 239000007788 liquid Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 210000003995 blood forming stem cell Anatomy 0.000 description 3
- 238000001125 extrusion Methods 0.000 description 3
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a freeze thawing box for melting freeze-stored umbilical cord hematopoietic stem cells, mainly relates to freeze thawing box field. Including the box body and the lid that is located the side-turning formula at box body top, the inside cavity of box body, the box body top is equipped with a plurality of jacks that communicate with the box body is inside, in the box body and jack bottom be equipped with a plurality of pipe brackets that correspond with the jack, place in jack and the pipe bracket and freeze and deposit the pipe, the box body lateral wall is opened has a plurality of through-holes that communicate with the box body is inside, be equipped with a plurality of elastic fastening devices who suits with freezing and deposit the pipe in the lid. The beneficial effects of the utility model reside in that: each freezing tube in the freezing and thawing box can be uniformly heated, so that the hematopoietic stem cells in the freezing tubes can be melted timely, continuously, uniformly and rapidly, water in the water bath can be prevented from entering the freezing tubes, and the pollution of cells in the freezing tubes is prevented.
Description
Technical Field
The utility model relates to a freeze thawing box field specifically is a freeze thawing box for melting freeze-stored umbilical cord hematopoietic stem cell.
Background
After being collected and processed, the umbilical cord hematopoietic stem cells are centralized and stored in a liquid nitrogen tank at the temperature of-196 ℃. In order to monitor the quality of the cord blood stem cells, workers will take out the cryovial from a liquid nitrogen reservoir at the temperature of-196 ℃ periodically and melt the cryovial in a water bath at the temperature of 37 ℃. The cell thawing time of the cryopreservation tube is short, the operation is soft, and the damage to the cells is avoided.
In general operation, because the temperature difference between the liquid nitrogen warehouse and the water bath is very different, in order to avoid the injury of workers caused by the burst of the freezing and storing pipe, the workers can directly throw the freezing and storing pipe taken out of the liquid nitrogen warehouse or the freezing and thawing box which is conventionally used in the market into the water bath kettle at 37 ℃. However, because the freezing and thawing box is of a closed structure, the freezing and thawing tubes near the edges in the freezing and thawing box can be heated more quickly than the freezing and thawing tubes in the middle, so that the freezing and thawing tubes are heated unevenly, and cannot be thawed timely, continuously, uniformly and quickly, and the quality of cells is influenced. In addition, water in the freezing and thawing box in the water bath pot can overflow the mouth of the freezing and thawing pipe, so that the pollution of cells in the freezing and thawing pipe is easily caused.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a freeze thawing box for melting freeze storage umbilical cord hematopoietic stem cell, it guarantees that each in the freeze thawing box freezes to deposit the pipe and can both the thermally equivalent, makes the intraductal hematopoietic stem cell of freezing to deposit obtain timely, last, even, rapid melting, and can avoid the water entering in the water bath to freeze and deposit the pipe, prevents to freeze the pollution of depositing intraductal cell.
The utility model discloses a realize above-mentioned purpose, realize through following technical scheme:
the utility model provides a freeze thawing box for melting freeze storage umbilical cord hemopoietic stem cell, includes the box body and is located the lid of the formula of turning on one's side at box body top, the inside cavity of box body, the box body top is equipped with a plurality of jacks that communicate with the box body is inside, just jack bottom is equipped with a plurality of pipe holders that correspond with the jack in the box body, place in jack and the pipe holder and freeze and deposit the pipe, the box body lateral wall is opened has a plurality of through-holes that communicate with the box body is inside, be equipped with a plurality of elastic fastening devices that suit with freezing and deposit the pipe in the lid.
Furthermore, the elastic fastening device comprises a sleeve inserted into the top of the cryopreservation tube, a pressing plate is arranged in the sleeve in a sliding mode, and a spring is connected between the pressing plate and the inner end face of the sleeve.
Furthermore, a sliding groove is arranged in the sleeve, one end, close to the opening of the sleeve, of the sliding groove is closed, and the pressing plate is connected with the sliding groove in a sliding mode.
Furthermore, an elastic ring is arranged in the jack.
Further, the bottom of the box body is provided with a stirring rod and a stirring motor for driving the stirring rod to rotate, and the bottom of the box body is also provided with a temperature sensor.
Furthermore, a buckle for locking the box body and the box cover is arranged on the box cover.
Contrast prior art, the beneficial effects of the utility model reside in that:
1. the freezing pipes are inserted into the box body through the insertion holes, and the supporting effect is achieved through the pipe supports, after the box body is communicated with the freezing pipes and is completely placed into a water bath pot, warm water in the water bath pot enters the inner cavity of the box body from the through holes, so that each freezing pipe can be in contact with the warm water through the pipe support at the bottom, all the freezing pipes in the box body can be uniformly heated, hematopoietic stem cells in each freezing pipe can be timely, continuously, uniformly and rapidly melted, and the phenomenon that the hematopoietic stem cells in the freezing pipes at the edges are damaged after being excessively heated due to the fact that the edge melting speed is higher than the center is avoided;
2. the pipe bracket is when the pipe is deposited to the bearing freezing, also can play the water that prevents to get into the box inner chamber through the through-hole and spill into in the freezing pipe from the clearance of depositing pipe and jack, and after lid and box body lock, the mouth of pipe of depositing the pipe can firmly be pushed down to the elasticity fastener in the lid, and it is intraductal to further prevent that the water in box inner chamber or the water bath pot from spilling into the freezing, prevents to freeze and deposits intraductal hemopoietic stem cell by pollution.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic view of the structure of the present invention.
Fig. 3 is a side view of the structure of the present invention.
Fig. 4 is an enlarged view of a portion a of fig. 3 according to the present invention.
Reference numerals shown in the drawings:
1. a box body; 2. a box cover; 3. a jack; 4. a pipe bracket; 5. a through hole; 6. a sleeve; 7. pressing a plate; 8. a spring; 9. a chute; 10. an elastic ring; 11. a stirring rod; 12. a stirring motor; 13. a temperature sensor; 14. and (5) buckling.
Detailed Description
The present invention will be further described with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teachings of the present invention, and these equivalents also fall within the scope defined in the present application.
The utility model relates to a freeze thawing box for thawing umbilical cord hematopoietic stem cells for frozen storage, the main structure comprises a box body 1 made of transparent materials and a side-turning type box cover 2 positioned at the top of the box body 1, the box cover 2 is of a shell structure, the box body 1 is hollow, one side of the top of the box body 1, which is close to the box cover, is provided with a plurality of jacks 3 communicated with the inside of the box body 1, a plurality of pipe supports 4 corresponding to the jacks 3 are arranged in the box body 1 and at the bottoms of the jacks 3, frozen storage pipes are arranged in the jacks 3 and the pipe supports 4, the shape of the bottoms of the pipe supports 4 is matched with the shape of the bottoms of the frozen storage pipes, the stability of the frozen storage pipes is ensured, the side wall of the box body 1 is provided with a plurality of through holes 5 communicated with the inside of the box body 1, warm water in a water bath kettle is introduced into the inner cavity of the box body 1 through the through holes 5, so that each frozen storage pipe can be immersed in water, and the even heating of the frozen storage pipes is ensured, a plurality of elastic fastening devices which are matched with the cryopreservation tubes are arranged in the box cover 2, the cryopreservation tubes are placed in the box body 1, the box cover 2 is buckled with the box body 1, the elastic fastening devices are contacted with the tube mouth ends of the cryopreservation tubes, the cryopreservation tubes extrude and deform the elastic fastening devices, so that the elastic fastening devices are firmly pressed at the tube mouth parts of the cryopreservation tubes by means of elasticity to prevent water from entering the cryopreservation tubes to pollute cells,
insert the back from jack 3 with freezing and deposit the pipe in pipe bracket 4, with box body 1 and 2 locks of lid, make elasticity fastener rely on the mouth of pipe firmly extrusion shutoff that the pipe will be frozen and deposited to elasticity, place box body 1 whole in the water bath kettle, water passes through-hole 5 and gets into 1 inner chamber of box body, make all pipe brackets 4 all immerse the aquatic, thereby make all freezing and depositing the pipe all through pipe bracket 4 immerse the aquatic, make all freezing and depositing the pipe together thaw, guarantee that every intraductal hemopoietic stem cell of freezing and depositing obtains timely, last, even melting, and pipe bracket 4 can prevent that water from overflowing from jack 3 and going into freezing and depositing intraductally, prevent that the cell in the freezing and depositing pipe is polluted.
Preferably, elasticity fastener includes the sleeve pipe 6 of pegging graft with the cryopreserved pipe top, and after box body 1 and 2 locks of lid, cryopreserved pipe top can insert in the sleeve pipe 6, and the open end of sleeve pipe 6 just or not with 1 surface contact of box body, it is equipped with clamp plate 7 to slide in the sleeve pipe 6, be connected with spring 8 between clamp plate 7 and the terminal surface in the sleeve pipe 6, after 2 locks of lid and box body 1, cryopreserved pipe's nose end will clamp plate 7 to the intraductal extrusion slip of sleeve pipe 6, and spring 8 warp the shrink back at this moment and applys a thrust to 6 open end directions of sleeve pipe to clamp plate 7, makes clamp plate 7 firmly press the mouth of pipe that cryopreserved pipe will cryopreserved pipe shutoff, prevents that water from getting into the intraductal pollution cell of cryopreserved.
Preferably, be equipped with spout 9 in the sleeve pipe 6, the diameter of spout 9 is greater than the sleeve pipe 6 internal diameter and is less than the sleeve pipe 6 external diameter, the spout 9 is close to the open-ended one end of sleeve pipe 6 and is sealed, clamp plate 7 and spout 9 sliding connection, the diameter of clamp plate 7 is greater than the sleeve pipe 6 internal diameter and is less than the spout 9 diameter, makes clamp plate 7 can only slide along spout 9 in sleeve pipe 6, prevents that clamp plate 7 from droing from sleeve pipe 6, guarantees holistic normal use.
Preferably, be equipped with the elastic ring 10 in the jack 3, the elastic ring 10 can adopt the rubber material, the internal diameter of elastic ring 10 slightly is less than the external diameter that freezes and deposit the pipe, when freezing and depositing the pipe and passing elastic ring 10 and insert jack 3, the outer wall that freezes and deposit the pipe extrudees elastic ring 10 outside and makes elastic ring 10 atress warp, make elastic ring 10 will freeze and deposit the pipe firmly the extrusion and fix in jack 3 under the elastic force effect that self resumes deformation, prevent to freeze and deposit the pipe and rock in the conduit saddle 4, when improving the stability that freezes and deposits the pipe and place, guarantee sleeve 6 and freeze and deposit the quick butt joint of inserting of pipe end portion.
Preferably, 1 bottom of box body is equipped with puddler 11 and drive puddler 11 pivoted agitator motor 12, and agitator motor 12 installs in waterproof mounting box, still is equipped with the controller in the mounting box, 1 bottom of box body still is equipped with temperature sensor 13, monitors the temperature of box body 1 position through temperature sensor 13, when detecting that the temperature around the box body 1 drops to lower numerical value, send the signal for the controller, controller control agitator motor 12 drive puddler 11 rotates, the water that makes 1 position of box body is mixxed, so that make the lower temperature water around the box body 1 mix with the higher water of surrounding temperature, guarantee that box body 1 is in suitable temperature environment all the time, thereby make every hematopoietic stem cell in freezing the storage tube obtain lasting melting.
Preferably, the box cover 2 is provided with a buckle 14 for locking the box body 1 and the box cover 2, so that the box cover 2 is tightly buckled with the box body 1, the pressing plate 7 is tightly pressed with the mouth of the freezing storage pipe, and water is prevented from overflowing into the freezing storage pipe to pollute cells.
This patent makes all of the cryopreserved pipes in the box body 1 can thermally equivalent, makes every cryopreserved intraductal hematopoietic stem cell obtain in time, last, even, rapid melting, prevents that the edge from melting fast in the center and leading to the edge cryopreserved intraductal hematopoietic stem cell impaired after excessively being heated, and prevent that the water in 1 inner chamber of box body or the water bath pot from overflowing to the cryopreserved intraductal, prevents that the intraductal hematopoietic stem cell of cryopreserved from being polluted.
Claims (6)
1. The utility model provides a freeze thawing box for thawing cryopreserved umbilical cord hematopoietic stem cells, includes box body (1) and lid (2) that are located the side-turning formula at box body (1) top, its characterized in that: the inside cavity of box body (1), box body (1) top is equipped with a plurality of jacks (3) with the inside intercommunication of box body (1), just jack (3) bottom is equipped with a plurality of pipe brackets (4) that correspond with jack (3) in box body (1), place in jack (3) and pipe bracket (4) and freeze and deposit the pipe, box body (1) lateral wall is opened has a plurality of through-holes (5) with the inside intercommunication of box body (1), be equipped with a plurality of and freeze the elasticity fastener that deposits the pipe and suit in lid (2).
2. The freeze-thaw cassette for thawing cryopreserved umbilical cord hematopoietic stem cells according to claim 1, wherein: the elastic fastening device comprises a sleeve (6) inserted with the top of the cryopreservation tube, a pressing plate (7) is arranged in the sleeve (6) in a sliding mode, and a spring (8) is connected between the pressing plate (7) and the inner end face of the sleeve (6).
3. The freeze-thaw cassette for thawing cryopreserved umbilical cord hematopoietic stem cells according to claim 2, wherein: the improved structure is characterized in that a sliding groove (9) is arranged in the sleeve (6), one end, close to the opening of the sleeve (6), of the sliding groove (9) is closed, and the pressing plate (7) is in sliding connection with the sliding groove (9).
4. The freeze-thaw cassette for thawing cryopreserved umbilical cord hematopoietic stem cells according to claim 1, wherein: an elastic ring (10) is arranged in the jack (3).
5. The freeze-thaw cassette for thawing cryopreserved umbilical cord hematopoietic stem cells according to claim 1, wherein: the stirring device is characterized in that a stirring rod (11) and a stirring motor (12) driving the stirring rod (11) to rotate are arranged at the bottom of the box body (1), and a temperature sensor (13) is further arranged at the bottom of the box body (1).
6. The freeze-thaw cassette for thawing cryopreserved umbilical cord hematopoietic stem cells according to claim 1, wherein: the box cover (2) is provided with a buckle (14) for locking the box body (1) and the box cover (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120046701.4U CN214193205U (en) | 2021-01-08 | 2021-01-08 | Freezing and thawing box for thawing cryopreserved umbilical cord hematopoietic stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120046701.4U CN214193205U (en) | 2021-01-08 | 2021-01-08 | Freezing and thawing box for thawing cryopreserved umbilical cord hematopoietic stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214193205U true CN214193205U (en) | 2021-09-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120046701.4U Expired - Fee Related CN214193205U (en) | 2021-01-08 | 2021-01-08 | Freezing and thawing box for thawing cryopreserved umbilical cord hematopoietic stem cells |
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| Country | Link |
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| CN (1) | CN214193205U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114703137A (en) * | 2021-12-31 | 2022-07-05 | 浙江金时代生物技术有限公司 | Method for differentiating umbilical cord mesenchymal stem cells into neural stem cells by utilizing cryopreserved umbilical cord mesenchymal stem cells |
-
2021
- 2021-01-08 CN CN202120046701.4U patent/CN214193205U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114703137A (en) * | 2021-12-31 | 2022-07-05 | 浙江金时代生物技术有限公司 | Method for differentiating umbilical cord mesenchymal stem cells into neural stem cells by utilizing cryopreserved umbilical cord mesenchymal stem cells |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210914 |