CN214115566U - Multilayer cell culture factory and supernatant suction device thereof - Google Patents
Multilayer cell culture factory and supernatant suction device thereof Download PDFInfo
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- CN214115566U CN214115566U CN202022520417.4U CN202022520417U CN214115566U CN 214115566 U CN214115566 U CN 214115566U CN 202022520417 U CN202022520417 U CN 202022520417U CN 214115566 U CN214115566 U CN 214115566U
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Abstract
The utility model relates to a multilayer cell culture factory and a supernatant fluid suction device thereof, wherein the multilayer cell culture factory comprises a plurality of rectangular box bodies which are arranged in a stacking way, and two corners of one side of each box body are respectively provided with an air inlet channel and an air outlet channel; the other layers of the box bodies except the top layer of the box body are provided with liquid drainage channels which correspond in position and are communicated up and down, the liquid drainage channels are communicated with the culture area in the middle of the box body through liquid outlet holes on the lateral parts, the lower ends of the liquid drainage channels of the bottom layer of the box body are closed, and liquid outlets communicated with the liquid drainage channels are arranged on the lateral parts of the liquid drainage channels. The utility model discloses multilayer cell culture mill has possessed the supernatant discharge function, convenient to use, easily operation, and the practicality is strong, the weeping problem that produces when can not appearing empting.
Description
Technical Field
The utility model relates to a multilayer cell culture mill and supernatant suction means thereof.
Background
The cell culture factory is a cell culture device, and the culture surface is enlarged to the maximum extent in a limited space, so that the space is saved, and the culture quantity is greatly increased; particularly suitable for moderate-scale production of lentiviral vectors in adherent cells. However, since the cell culture factory is a hierarchical structure, the supernatant in the culture of the lentiviral vector can only be discharged by pouring, the operation is troublesome, and the leakage of the supernatant is easily caused in the pouring process.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing a convenient to use, easy operation, multilayer cell culture mill and supernatant suction device thereof that the practicality is strong.
The utility model discloses a following scheme realizes: a multilayer cell culture factory comprises a plurality of rectangular box bodies which are arranged in a stacked mode, wherein an air inlet channel and an air outlet channel are formed in two corners of one side of each box body respectively; the other layers of the box bodies except the top layer of the box body are provided with liquid drainage channels which correspond in position and are communicated up and down, the liquid drainage channels are communicated with the culture area in the middle of the box body through liquid outlet holes on the lateral parts, the lower ends of the liquid drainage channels of the bottom layer of the box body are closed, and liquid outlets communicated with the liquid drainage channels are arranged on the lateral parts of the liquid drainage channels.
Furthermore, except the top layer box body, one side of each layer of box bodies is provided with a boss which corresponds in position and protrudes inwards, the liquid drainage pore passage and the liquid outlet hole are positioned in the boss, and the upper end face of the boss is flush with the opening end face of the box body and clings to the bottom face of the upper layer box body.
Further, the port department of liquid outlet is provided with the first bell mouth that one end is big outwards, one end is little inwards, the inside second cone hole that is big outwards, one end is little inwards that is provided with outwards and is used for plugging up the end cap in second cone hole, and the end cap outside is provided with the spring that pushes up the end cap to second cone hole direction.
Further, except that the top layer box body, the edge of the opening end face of each layer of box body is provided with a step groove with a circle of L-shaped section, the step groove is connected with the bottom of the box body stacked on the step groove, and the upper end of the air inlet channel and the upper end of the air outlet channel are lower than the height of the opening end face of the box body.
The utility model discloses another technical scheme: a supernatant liquid suction device for the multilayer cell culture factory comprises a base for placing the multilayer cell culture factory and a pipette for sucking supernatant liquid in the multilayer cell culture factory, wherein the lower end of the pipette is connected with a pipette matched with a liquid outlet of the multilayer cell culture factory in an inserted manner, the upper end of the pipette is connected with a liquid suction hose leading to an inlet of a liquid suction pump, and an outlet of the liquid suction pump is connected with a liquid outlet pipe leading to a supernatant liquid storage bottle; the base rear side fixedly connected with vertical pole, vertical pole upper end is provided with the horizontal pole that is used for placing the pipette.
Further, the lateral part of the upper end of the pipette is provided with an L-shaped hook used for being hung on the cross rod, the lower end of the L-shaped hook is provided with an arc bending part which is bent outwards, and the end part of the cross rod is provided with a limit stop.
Furthermore, the upper end of the pipette is provided with a liquid feeding port connected with a liquid suction hose, the lower end of the pipette is provided with a liquid inlet used for being connected with the pipette, the liquid outlet pipe is provided with a filter head, and the pipette is provided with a one-way valve which only allows liquid to enter the handheld pipe body in a one-way mode.
Furthermore, a transverse plate located on the opposite side of the transverse rod is arranged at the upper end of the vertical rod, the liquid suction pump is installed on the transverse plate, and a positioning ring for allowing the liquid suction hose to penetrate through is arranged at the upper end of the vertical rod.
Compared with the prior art, the utility model discloses following beneficial effect has: the multi-layer cell culture factory has the function of discharging the supernatant, is convenient to use, easy to operate and strong in practicability, and can avoid the problem of liquid leakage generated during pouring; simultaneously with multilayer cell culture factory cooperation work's supernatant suction means structural design simple reasonable, after automatic the absorbing supernatant of imbibition pump and send into the supernatant storage bottle, save time, efficient.
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to specific embodiments and related drawings.
Drawings
FIG. 1 is a schematic diagram of a multi-layered cell culture factory according to an embodiment of the present invention;
FIG. 2 is a top view of a cassette of a multi-layered cell culture factory according to an embodiment of the present invention;
FIG. 3 is a schematic view of a portion of the construction of FIG. 1;
FIG. 4 is a schematic view of the whole structure of the supernatant suction device according to the embodiment of the present invention;
fig. 5 is a schematic diagram of a pipette configuration of a supernatant suction device according to an embodiment of the present invention;
the reference numbers in the figures illustrate: 100-base, 110-vertical rod, 111-horizontal rod, 112-limit stop, 113-transverse plate, 114-positioning ring, 200-pipette, 210-L-shaped hook, 211-arc bending part, 220-pipette, 221-taper part, 230-one-way valve, 300-pipette pump, 310-pipette hose, 320-drain pipe, 321-filter, 400-supernatant storage bottle, 500-multilayer cell culture factory, 510-box, 511-air inlet channel, 512-air outlet channel, 513-liquid discharge channel, 514-liquid outlet, 515-boss, 516-step groove, 520-liquid outlet, 521-first taper hole, 522-second taper hole, 523-plug and 524-spring.
Detailed Description
As shown in fig. 1 to 3, a multi-layered cell culture factory includes a plurality of rectangular box bodies 510 stacked one on another, wherein two corners of one side of each box body 510 are respectively provided with an air inlet channel 511 and an air outlet channel 512; all the other all the layers of box bodies except the top layer box body are provided with liquid drainage channels 513 which correspond in position and are communicated up and down, the liquid drainage channels are communicated with a culture area in the middle of the box body through liquid outlet holes 514 of the side parts, the lower end of the liquid drainage channels of the bottom layer box body is closed, the outer side parts of the liquid drainage channels are provided with liquid outlet holes 520 communicated with the liquid drainage channels, valves can be arranged on the liquid outlet holes to control opening and closing, the liquid drainage channels 513 on all the layers of box bodies are communicated, supernatant in all the layers of box bodies can automatically flow into the liquid distribution channels, when the liquid outlet holes are opened, the supernatant can be discharged from the liquid outlet holes, the supernatant of all the layers of box bodies of the whole multilayer cell culture factory can be discharged simultaneously, and the supernatant is collected conveniently after being collected at one liquid outlet hole, the use is convenient, the operation is easy, the practicability is strong, and the liquid leakage condition can not occur.
In this embodiment, all the other each layer box body one side is provided with the boss 515 that the position corresponds and inside bellied except that the top layer box body, flowing back pore and play liquid hole are located the boss, and boss up end and box body opening terminal surface parallel and level hug closely the bottom surface of upper box body, except that the bottom box body, all the other flowing back pore on the box body of taking the boss link up boss up end and box body bottom surface, and the outage on the bottom box body only link up the boss up end, and the lower extreme then does not link up the box body bottom surface.
In this embodiment, the liquid outlet can be opened and closed by setting a valve, and the following method can be adopted: the port department of liquid outlet 520 is provided with outside one end big, the little first bell mouth 521 of one end inwards, the liquid outlet is inside to be provided with outside one end big, the little second cone hole 522 of one end inwards and to be used for plugging up the end cap 523 of second cone hole, the end cap outside is provided with the spring 524 that pushes away the end cap to second cone hole direction, under normal conditions, receiving spring 524's effect, end cap 523 blocks up second cone hole, and make the liquid outlet close, when being connected with the liquid outlet through suction equipment and absorbing the supernatant, receive vacuum suction effect, the spring can be compressed and make the end cap keep away from the conical surface in second cone hole, and then open liquid outlet discharge supernatant.
In this embodiment, all the other each layer box body opening end face borders are provided with the ladder groove 516 that the round cross-section is the L type except that the top layer box body, the ladder groove is in the same place with the box body bottom joint that stacks above that, and upper box body bottom is through realizing sealedly with lower floor box body ladder groove extrusion back joint together, through inlet channel and outlet channel upper end height are less than box body opening end face height for form the interval between inlet channel and outlet channel upper end and the box body bottom surface on upper strata, inlet channel and outlet channel just are through the cultivation district intercommunication in this interval and the box body.
As shown in fig. 4 to 5, a supernatant suction device for the multi-layered cell culture factory comprises a base 100 for placing the multi-layered cell culture factory and a pipette 200 for sucking the supernatant in the multi-layered cell culture factory, wherein the lower end of the pipette 200 is connected with a pipette 230 which is in plug fit with a liquid outlet 520 of the multi-layered cell culture factory, the upper end of the pipette 200 is connected with a liquid suction hose 310 leading to an inlet of a liquid suction pump 300, an outlet of the liquid suction pump 300 is connected with a liquid outlet 320 leading to a supernatant storage bottle 400, and the liquid outlet of the multi-layered cell culture factory can be forced to be automatically opened by the suction force of the liquid suction pump 300; the rear side of the base 100 is fixedly connected with a vertical rod 110, and the upper end of the vertical rod 110 is provided with a cross rod 111 for placing the pipette 200. Multilayer cell culture mill places on the base, open the imbibition pump, the staff is through handheld pipette, the pipette 230 of pipette is pegged graft on the liquid outlet of multilayer cell culture mill, after drawing the supernatant automatically through the imbibition pump and send into in the supernatant storage bottle, can carry out the supernatant to all box bodys on whole multilayer cell culture mill simultaneously and draw the operation, the operation of pouring the supernatant is carried out to multilayer cell culture mill to having saved, the imbibition pump can be in operating condition always, treat that whole batch multilayer cell culture mill draws and closes the imbibition pump after finishing, this supernatant suction means structural design is simple reasonable, high durability and convenient use, easy operation, therefore, the clothes hanger is strong in practicability, save time, high efficiency.
In this embodiment, the side portion of the upper end of the pipette 200 is provided with an L-shaped hook 210 for hanging on the cross bar, and the lower end of the L-shaped hook 210 is provided with an arc bending portion 211 bending outwards, so as to facilitate quick hanging of the pipette; the end of the cross rod 111 is provided with a limit stop 112 to prevent the pipette from falling off because the pipette is not hung clearly.
In this embodiment, the upper end of the pipette 200 is provided with a liquid feeding port connected with a liquid suction hose, the lower end of the pipette 200 is provided with a liquid inlet for connecting with the pipette 230, the liquid outlet pipe is provided with a filter 321, and the aperture of the filter 230 is 0.45 um or 0.22 um, which can be replaced as required; the liquid suction pipe is provided with a one-way valve 230 which only allows liquid to enter the handheld pipe body in one way, so that supernatant liquid is prevented from flowing back to the culture dish; the lower end of the liquid suction pipe 230 is provided with a tapered portion 231 which is matched with the first tapered hole of the liquid outlet 520, so as to ensure the sealing performance when the liquid suction pipe and the liquid outlet are plugged.
In this embodiment, the upper end of the vertical rod 110 is provided with a transverse plate 113 located on the opposite side of the transverse rod, the pipette pump 300 is installed on the transverse plate 113, and the upper end of the vertical rod 110 is provided with a positioning ring 114 for the pipette hose to pass through, so as to prevent the pipette hose from hanging down and affecting the pipette operation.
Any technical solution disclosed in the present invention is, unless otherwise stated, disclosed a numerical range if it is disclosed, and the disclosed numerical range is a preferred numerical range, and any person skilled in the art should understand that: the preferred ranges are merely those values which are obvious or representative of the technical effect which can be achieved. Because numerical value is more, can't be exhaustive, so the utility model discloses just disclose some numerical values with the illustration the technical scheme of the utility model to, the numerical value that the aforesaid was enumerated should not constitute right the utility model discloses create the restriction of protection scope.
The utility model discloses if disclose or related to mutual fixed connection's spare part or structure, then, except that other the note, fixed connection can understand: a detachable fixed connection (for example using bolts or screws) is also understood as: non-detachable fixed connections (e.g. riveting, welding), but of course, fixed connections to each other may also be replaced by one-piece structures (e.g. manufactured integrally using a casting process) (unless it is obviously impossible to use an integral forming process).
In addition, the terms used in any aspect of the present disclosure as described above to indicate positional relationships or shapes include similar, analogous, or approximate states or shapes unless otherwise stated.
The utility model provides an arbitrary part both can be assembled by a plurality of solitary component parts and form, also can be the solitary part that the integrated into one piece technology was made.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the same; although the present invention has been described in detail with reference to preferred embodiments, it should be understood by those skilled in the art that: the invention can be modified or equivalent substituted for some technical features; without departing from the spirit of the present invention, it should be understood that the scope of the claims is intended to cover all such modifications and variations.
Claims (8)
1. A multilayer cell culture factory, includes the rectangle box body of a plurality of range upon range of settings, its characterized in that: an air inlet channel and an air outlet channel are respectively arranged at two corners of one side of the box body; the other layers of the box bodies except the top layer of the box body are provided with liquid drainage channels which correspond in position and are communicated up and down, the liquid drainage channels are communicated with the culture area in the middle of the box body through liquid outlet holes on the lateral parts, the lower ends of the liquid drainage channels of the bottom layer of the box body are closed, and liquid outlets communicated with the liquid drainage channels are arranged on the lateral parts of the liquid drainage channels.
2. The multi-layered cell culture factory according to claim 1, characterized in that: except the top box body, one side of each layer of box bodies is provided with a boss which corresponds in position and protrudes inwards, the liquid drainage pore passage and the liquid outlet hole are positioned in the boss, and the upper end face of the boss is flush with the opening end face of the box body and clings to the bottom face of the upper box body.
3. The multi-layered cell culture factory according to claim 1 or 2, characterized in that: the port department of liquid outlet is provided with the first bell mouth that one end is big outwards, one end is little inwards, the inside second taper hole that is big outwards one end, one end is little inwards and is used for plugging up the end cap in second taper hole that is provided with outwards one end, and the end cap outside is provided with the spring that pushes away the end cap to second taper hole direction.
4. The multi-layered cell culture factory according to claim 1, characterized in that: except that top layer box body all the other each layer box body opening end face border is provided with the ladder groove that the round cross-section is the L type, the ladder groove is in the same place with the box body bottom joint that stacks above that, inlet channel and outlet channel upper end height are less than box body opening end face height.
5. A supernatant suction device for use in the multi-layered cell culture factory according to claim 1, wherein: the device comprises a base used for placing a multilayer cell culture factory and a pipette used for sucking supernatant in the multilayer cell culture factory, wherein the lower end of the pipette is connected with a pipette matched with a liquid outlet of the multilayer cell culture factory in an inserted manner, the upper end of the pipette is connected with a liquid suction hose leading to an inlet of a liquid suction pump, and an outlet of the liquid suction pump is connected with a liquid outlet pipe leading to a supernatant storage bottle; the base rear side fixedly connected with vertical pole, vertical pole upper end is provided with the horizontal pole that is used for placing the pipette.
6. The supernatant extraction device of claim 5, wherein: the pipette is characterized in that an L-shaped hook used for being hung on the cross rod is arranged on the side portion of the upper end of the pipette, an arc-shaped bending portion which is bent outwards is arranged at the lower end of the L-shaped hook, and a limit stop is arranged at the end portion of the cross rod.
7. The supernatant extraction device of claim 6, wherein: the liquid suction device is characterized in that a liquid feeding port connected with a liquid suction hose is formed in the upper end of the pipette, a liquid inlet used for being connected with the pipette is formed in the lower end of the pipette, a filter head is installed on the liquid outlet pipe, and a check valve allowing liquid to enter the handheld pipe body in a one-way mode is formed in the pipette.
8. The supernatant extraction device of claim 5, wherein: the upper end of the vertical rod is provided with a transverse plate positioned on one side opposite to the transverse rod, the imbibition pump is installed on the transverse plate, and the upper end of the vertical rod is provided with a positioning ring for the imbibition hose to pass through.
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CN202022520417.4U CN214115566U (en) | 2020-11-04 | 2020-11-04 | Multilayer cell culture factory and supernatant suction device thereof |
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CN202022520417.4U CN214115566U (en) | 2020-11-04 | 2020-11-04 | Multilayer cell culture factory and supernatant suction device thereof |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113924892A (en) * | 2021-11-08 | 2022-01-14 | 辰溪县千鑫生态农业科技发展有限公司 | Auxiliary device is cultivated to rice |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113924892A (en) * | 2021-11-08 | 2022-01-14 | 辰溪县千鑫生态农业科技发展有限公司 | Auxiliary device is cultivated to rice |
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