CN214085738U

CN214085738U - Reagent storage device and sample application instrument

Info

Publication number: CN214085738U
Application number: CN202022016533.2U
Authority: CN
Inventors: 汪云飞; 潘能科
Original assignee: Chongqing Topu Bioengineering Co Ltd
Current assignee: Chongqing Topu Bioengineering Co Ltd
Priority date: 2020-09-15
Filing date: 2020-09-15
Publication date: 2021-08-31
Anticipated expiration: 2030-09-15

Abstract

The utility model relates to sample pretreatment, and discloses a reagent storage device and a sample applicator, wherein the reagent storage device comprises a reagent storage structure, a driving structure and a cover for sealing the reagent storage structure, and can seal the reagent, thereby effectively slowing down the volatilization and deterioration of the reagent; and the matched movement of the cover and the sample application needle is utilized to prevent the sample application needle from being damaged due to the firing pin, so that the sample application dosage is prevented from being influenced, and the sample application accuracy is ensured.

Description

Reagent storage device and sample application instrument

Technical Field

The utility model relates to a sample pretreatment, concretely relates to reagent storage device and sample application appearance.

Background

Mass spectrometry is an analytical method by which the mass-to-charge ratio (m/z) of ions of a sample to be measured is determined for analysis. The sample to be analyzed is ionized by an ion source, ions are separated according to m/z difference by utilizing different motion behaviors of different ions in an electric field or a magnetic field to obtain a mass spectrum, and qualitative and quantitative results of the sample are obtained through a mass spectrogram of the sample. The matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF) is a branch of the mass spectrometer, and can quickly and accurately analyze and identify a plurality of samples at the same time, wherein the samples are usually spotted on a target spot and then sent into the matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF) for analysis and identification.

However, most of the existing sample application is completed manually, and the working mode has low efficiency, low sample application precision and possibility of error point generation, thereby causing sample rejection. Meanwhile, the sample-printed target plate is directly used for mass spectrometer analysis, and the analysis result of the mass spectrometer is closely related to the sample-printed target plate. The problem of low sample application precision can cause negative influence on the detection and analysis result at present.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the not enough of prior art, the utility model provides a reagent storage device and sample application appearance can automatic sample application to improve work efficiency, and can improve the precision of sample application, with the accuracy of guaranteeing the result of detection and analysis.

The technical scheme of the utility model as follows:

an aspect of the present invention relates to a reagent storage device, including drive structure and the lid that is used for sealed reagent to deposit the structure, shown lid is connected with drive structure, drive structure drive the lid removes.

The cover moves in a direction at an angle to the upper surface of the reagent storage device.

By "having an angle" is meant an angle greater than 0.

Preferably, the driving structure comprises a motor and a connecting structure, wherein one end of the connecting structure is connected to the motor, and the other end of the connecting structure is connected to the cover.

Preferably, the motor is a rotating motor, and an axis of a rotating shaft of the motor is in a vertical direction or a horizontal direction.

Preferably, the reagent storage device still includes the support, the support includes motor fore-stock and motor after-poppet, the motor is installed the motor fore-stock with between the motor after-poppet, the motor fore-stock with the motor after-poppet is installed in the frame.

Preferably, the number of the trigger pieces is more than one, and the number of the sensors is two.

Preferably, the connecting structure comprises a link structure connecting the cover and the motor.

Optionally, the link structure includes a first link structure and a second link structure, one end of the first link structure is rotatably connected to the slider, the other end of the first link structure is rotatably connected to one end of the second link structure, the other end of the second link structure is rotatably connected to the reagent storage structure, and the cover is fixed to the first link structure or the second link structure.

Preferably, the angle between the first and second links is less than 180 °.

Preferably, the connecting structure is provided with a trigger piece, the reagent storage device further comprises a support and a sensor, the sensor is mounted on the support, the support is fixed on the rack, and the sensor is matched with the trigger piece in position.

"looks adaptation": it means that the sensor can generate signal change due to the movement of the trigger sheet during the movement of the connection structure, namely the trigger sheet controls the connection or disconnection of the sensor signal, thereby transmitting the switch state of the cover to the system.

Preferably, a sealing element is fixed on the surface of the cover facing the reagent storage structure, the sealing element can be made of any material with a sealing effect, preferably a rubber material, and the rubber layer is in direct contact with the mouth of the reagent bottle during sealing, so that the sealing effect can be enhanced.

The cover material can be suitable material, preferred aluminum alloy material, and aluminum alloy material density is little intensity height, can reduce the load of motor when guaranteeing intensity.

The reagent storage structure is provided with one or more holes, and the number of the holes is freely selected according to the number of the carried reagents.

Another aspect of the present invention relates to a sample applicator, which comprises the above reagent storage device.

Preferably, the spotting instrument further comprises a spotting needle and a control system for controlling the spotting needle and the driving structure to move, wherein the control system and the spotting needle are both arranged on the frame, and the spotting needle is controlled by the control system to be matched with the cover in a rotating way.

"sample application needle is controlled by control system control and lid and is rotated to cooperate" and indicates: the sensor monitors the motion state of the cover and transmits signals to the control system of the sample applicator, the control system outputs feedback signals to the sample application needle to control the motion of the sample application needle, and the motion condition of the sample application needle depends on the motion state of the cover.

The purpose of the print needle in cooperation with the movement of the cover is to prevent a needle from being fired.

The utility model discloses a "firing pin": the sample application needle of the instrument collides with other parts of the instrument, causing distortion, damage and the like of the sample application needle.

In an embodiment of the present invention, the connection structure is a trigger piece, the sensor includes a first photoelectric switch and a second photoelectric switch, and the first photoelectric switch and the second photoelectric switch are disposed side by side. When the cover is completely covered, the first photoelectric switch signal is cut off by the trigger piece, the second photoelectric switch keeps on light, and the sample application needle keeps a static state and cannot move downwards; when the cover is completely opened, the signal of the second photoelectric switch is cut off by the trigger piece, the first photoelectric switch is powered on, and the sample needle moves downwards to perform sampling operation; when the cover is in other states except the two states, the signals of the two photoelectric switches are communicated, the sample application needle is in a light-on state and does not move, and the sample application needle is prevented from colliding with the cover.

In another embodiment of the present invention, the connecting structure includes a slider, a connecting rod structure and a trigger piece, the trigger piece is mounted on the slider; the sensor comprises a first photoelectric switch and a second photoelectric switch, and the first photoelectric switch and the second photoelectric switch are arranged side by side and are installed on the rack. When the cover needs to be closed, the motor drives the sliding block to move towards the motor, the first connecting rod structure pulls the second connecting rod structure leftwards, so that the second connecting rod structure drives the cover to be closed, and when the trigger piece cuts off the first photoelectric on signal, the cover is completely closed; when the cover needs to be opened, the motor drives the sliding block to move towards the direction of the reagent storage structure, the first connecting rod structure pushes the second connecting rod structure rightwards, so that the second connecting rod structure drives the cover to be opened, and when the trigger piece cuts off a signal of the second photoelectric switch, the cover is completely opened.

The utility model has the advantages that:

a large number of experiments show that the reason that the sample application precision is not high includes factors such as the concentration of the reagent, unstable components, dosage accuracy and the like besides the sample application position.

In the manual sample application process or the existing sample application instrument, the storage of sample application reagents is not taken into account, and only a working position for placing the sample application reagents is provided. Since the substrate components are volatile, the substrate can be deteriorated and cannot be used. The amount of each sample application is very small (1 microliter or less per target), and a small bottle of reagent is usually used for a long time, so that effective sealing is important for ensuring the sample application quality and saving the reagent. The utility model discloses a reagent storage device can seal reagent, has effectively slowed down volatilizing of reagent rotten.

In addition, the accuracy of artifical spotting that hardly controls the point appearance volume, the utility model discloses a reagent storage device adopts full-automatic point appearance, avoids artifical error, and the point appearance needle receives sensor signal's control simultaneously, can remove to cooperate with the lid, prevents to cause the damage for the point appearance needle because the firing pin, influences the point appearance dose, guarantees the accuracy of point appearance.

Because the moving direction of the cover is at an angle with the upper surface of the reagent storage device, when the cover is to be covered, the cover cuts into and adheres to the upper surface of the reagent storage device at a certain angle. The mode avoids untight sealing caused by continuous movement after the cover is contacted with the upper surface of the reagent storage device, and can avoid abrasion of the cover or the rubber pad and prolong the service life.

Drawings

Fig. 1 is a schematic structural diagram of embodiment 1 of the present invention;

fig. 2 is a schematic structural diagram of embodiment 2 of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples.

An object of the utility model is to overcome prior art not enough, provide a reagent storage device and sample application appearance, not only can realize the sealed save to matrix reagent, can also prevent the firing pin and keep instrument stability.

The sensor and the trigger piece are used for feeding back the position information of the cover, when the trigger piece triggers the sensor, the position information of one cover can be obtained, and two sensors are selected for completely feeding back two limit states of full opening and full covering of the cover. When the cover is in the fully covered position, the trigger plate cuts off the first sensor signal, and the second sensor is kept connected; when the cover is fully opened, the trigger plate cuts off the second sensor signal and the first sensor remains connected.

Example 1

As shown in fig. 1, an embodiment of the present invention provides a reagent storage device, which includes a driving structure 1, a cover 2, a sensor 3, a support 4, and a reagent storage structure 5.

The number of trigger pieces in this embodiment is 2.

The driving structure 1 comprises a connecting structure 11 and a motor 12, one end of the connecting structure 11 is connected to the motor 12, the other end of the connecting structure 11 is connected to the cover 2, and the connecting structure 11 further comprises a trigger piece 111 and a trigger piece 112.

The bracket 4 comprises a motor front bracket 41 and a motor rear bracket 42, and the motor front bracket 41 and the motor rear bracket 42 are both fixed on the frame, so that the structure is stable.

The motor 12 is fixed between the motor front bracket 41 and the motor rear bracket 42, and the

trigger pieces

111 and 112 are mounted on the connecting structure 11.

The lid 2 includes a lid plate 21 and a rubber pad 22, the rubber pad 22 is fixed below the lid plate 21 toward the reagent storage structure 4, and when sealing, the rubber pad 22 is in direct contact with the opening 41, and the sealing ability is improved by the elasticity of the rubber.

In this embodiment, the axis of the rotating shaft of the motor 12 is horizontal, and when the reagent needs to be stored in a sealed manner, the cover 2 is rotated along a vertical plane under the control of the motor 12. The vertically falling lid 2 can be made more airtight by its own weight.

The sensor 3 in this embodiment includes a photoelectric switch 31 and a photoelectric switch 32, and the photoelectric switch 31 and the photoelectric switch 32 are arranged side by side on the motor front bracket 111. When the cover is completely covered, the signal of the photoelectric switch 31 is cut off by the trigger sheet 111, the photoelectric switch 32 keeps on light, and the received signal is fed back to the control system, so that the sample application needle is controlled to keep a static state and cannot move downwards; when the cover is completely opened, namely rotates 180 degrees, the signal of the photoelectric switch 32 is cut off by the trigger sheet 112, the photoelectric switch 31 is powered on, and the received signal is fed back to the control system, so that the sample application needle is controlled to move downwards to perform sampling operation; when the cover is in other states except the two states, the signals of the two photoelectric switches are communicated, the cover is in a light-on state, the sample application needle is not moved, and the sample application needle is prevented from colliding with the cover 2.

The reagent storage structure 5 is provided with a reagent storage hole and fixed on the frame. The reagent storage hole is used for placing a reagent, and one to a plurality of reagent storage holes can be arranged.

In this embodiment, the upper surface of the reagent storage device is in a horizontal plane, and "angled" means that the lid does not rotate or translate in a horizontal plane, and in this embodiment, the lid rotates in a vertical plane, at an angle to the upper surface of the reagent storage device. When the lid is ready to be closed, the lid cuts into and engages the upper surface of the reagent storage device at an angle. The mode avoids untight sealing caused by continuous movement after the cover is contacted with the upper surface of the reagent storage device, and can avoid abrasion of the cover or the rubber pad and prolong the service life.

Example 2

The present embodiment is different from embodiment 1 only in that the direction of the rotating shaft of the motor 12 is changed, the axial direction of the rotating shaft is changed from horizontal to vertical, the positions of the trigger piece and the sensor are correspondingly changed, the cover 2 is rotated on the horizontal plane, and the position information of the cover is fed back through the sensor and the trigger piece.

Example 3

As shown in FIG. 2, a reagent storage device includes a driving mechanism 1, a cover 2, a sensor 3, and a reagent storage mechanism 4.

The driving structure 1 comprises a connecting structure 11 and a motor 12, wherein one end of the connecting structure 11 is connected to the motor 12, and the other end of the connecting structure is connected to the cover 2. The motor 12 is a linear motor and includes a lead screw 121, a guide rail 122 and a lead screw kit 123.

The connecting structure 11 includes a slider 111 and a link structure including a first link structure 112, a second link structure 113, a third link structure 114, and a trigger piece 115. The slide block 111 is fixed on the screw rod suite 123, the screw rod 121 is driven to rotate through the rotation of the motor 12, so that the slide block 111 is driven to reciprocate on the guide rail 122, the first connecting rod structure 112 is fixed on the slide block 111, one end of the second connecting rod structure 113 is rotatably connected with the first connecting rod structure 112, the other end of the second connecting rod structure is rotatably connected with one end of the third connecting rod structure 114, and the other end of the third connecting rod structure 114 is connected with the reagent storage structure 4; the trigger piece 115 is disposed at the lower end of the slider 111 and moves as the slider 111 moves.

The number of trigger pieces in this embodiment is 1.

The lid 2 includes a lid plate 21 and a rubber gasket 22, the rubber gasket 22 is fixed below the lid plate 21 and faces the opening 41 of the reagent storage structure 4, and the rubber gasket 22 is in direct contact with the opening 41 during sealing, and the sealing ability is improved by the elasticity of the rubber.

The cover 2 is fixed to the second link structure or the third link structure. In this embodiment, the cover 2 is fixed on the second link structure 113, and when the linear motor 12 drives the slider 111 to move, the second link structure 113 and the third link structure 114 are driven to rotate, so as to drive the cover 2 to rotate, and control the opening or closing of the cover 2. In the initial state, the second link structure 113 and the third link structure 114 directly have a certain angle, so that the self-locking phenomenon can be avoided, and the rotation direction of the cover 2 can be preset through the initial included angle.

The drive structure 1 further comprises a support 5, the sensor 3 being fixed to the support 5.

The sensor 3 comprises a photoelectric switch 31 and a photoelectric switch 32, and the photoelectric switch 31 and the photoelectric switch 32 are arranged on the bracket side by side. When the cover is completely closed, the trigger sheet 115 cuts off the signal of the photoelectric switch 31, and the photoelectric switch 31 feeds back the received signal to the control system, so that the sample application needle is controlled to keep a static state and cannot move downwards; when the cover is completely opened, the trigger sheet 115 cuts off the signal of the photoelectric switch 32, and the photoelectric switch 32 feeds the received signal back to the control system, so that the sample application needle is controlled to move downwards to perform sampling operation; when the cover is in other states except the two states, signals of the photoelectric switch 31 and the photoelectric switch 32 are communicated, and the cover is in a light-passing state, the sample application needle is kept still, and the sample application needle is prevented from colliding on the cover 2 to cause damage.

The reagent storage structure 4 is provided with a reagent storage hole and fixed on the frame. The reagent storage hole is used for placing reagents, and one to a plurality of reagent bottle storage holes can be arranged.

Claims

1. A reagent storage device comprises a reagent storage structure and is characterized by further comprising a driving structure and a cover used for sealing the reagent storage structure, wherein the cover is connected with the driving structure, the driving structure drives the cover to move, and the moving direction of the cover has an angle with the upper surface of the reagent storage device.

2. The reagent storage device of claim 1 wherein the drive structure comprises a motor and a connecting structure, the connecting structure being connected to the motor at one end and to the cover at the other end.

3. The reagent storage device of claim 2 wherein the motor is a rotary motor, the axis of the rotary shaft of the motor being either vertical or horizontal.

4. The reagent storage device of claim 2 wherein the connection structure comprises a slider and a linkage structure, the linkage structure connecting a lid and the motor.

5. The reagent storage device of claim 4 wherein the linkage structure comprises a first linkage structure and a second linkage structure, the first linkage structure having one end pivotally connected to the slide and the other end pivotally connected to one end of the second linkage structure, the other end of the second linkage structure pivotally connected to the reagent storage structure, and the lid secured to either the first linkage structure or the second linkage structure.

6. The reagent storage device of claim 5 wherein the first and second links are angled less than 180 °.

7. The reagent storage device of any one of claims 2 to 6 further comprising a bracket and a sensor, wherein the sensor is mounted on the bracket, the bracket is fixed on the rack, the connecting structure is provided with a trigger piece, and the sensor is matched with the position of the trigger piece.

8. The reagent storage device of any one of claims 1 to 6 wherein the reagent storage structure is provided with one or more apertures; the cover is made of aluminum alloy materials, and a sealing piece is fixed on the surface of the cover facing the reagent storage structure.

9. A spotting instrument, characterized in that: comprising a frame and a reagent storage device according to claims 1-8 mounted on the frame.