CN214032536U

CN214032536U - Cell culture plate without damaging matrigel and cells

Info

Publication number: CN214032536U
Application number: CN202021304791.4U
Authority: CN
Inventors: 刘艺霖; 吴志鹏; 邱江; 张家盛; 温晓欣; 王得中; 王恒
Original assignee: Guangzhou Huayue Nephrology Regenerative Medicine Technology Co ltd
Current assignee: Guangzhou Huayue Nephrology Regenerative Medicine Technology Co ltd
Priority date: 2020-07-06
Filing date: 2020-07-06
Publication date: 2021-08-24
Anticipated expiration: 2030-07-06

Abstract

The utility model discloses a cell culture plate that does not destroy matrigel and cell is provided with the bottom plate, be provided with a plurality of culture hole and a plurality of operation groove on the bottom plate, culture hole and at least one be provided with the pellicle between the operation groove and pass through the pellicle communicates. According to the utility model discloses do not destroy matrigel and cell culture plate of cell, experiment operator can not cause destruction to cell and matrigel when changing the culture solution to reduce the probability of experiment failure, simplified the experiment degree of difficulty, saved the experimental time.

Description

Cell culture plate without damaging matrigel and cells

Technical Field

The utility model relates to a cell culture plate, in particular to a cell culture plate which does not destroy matrigel and cells.

Background

The cell culture plate is a common tool for culturing cells, the existing cell culture plate mainly comprises a bottom plate and a cover plate, culture holes are formed in the bottom plate, and after culture solution and matrigel are added into the culture holes, cells can be added and cultured.

In cell culture experiments, particularly in the cell culture experiments by the sandwich gel method, the maintenance of the sandwich structure of matrigel is the basis for the success of the experiments. However, because there is no component for protecting the matrix glue structure in the culture hole of the existing cell culture plate, when an experiment operator changes the culture solution (i.e., adds liquid into the culture hole or takes liquid from the culture hole), the gun head for adding liquid or taking liquid is easily touched by the cell and the matrix glue due to irregular or careless operation, and then the cell and the matrix glue are damaged, resulting in errors or even failures in the experiment, which not only improves the experiment difficulty, but also wastes the experiment time.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at solving one of the technical problem that exists among the prior art at least. Therefore, the utility model provides a do not destroy matrigel and cell culture plate of cell, experiment operator can not cause destruction to cell and matrigel when changing the culture solution to reduce the probability of experiment failure, simplified the experiment degree of difficulty, saved the experimental time.

According to the utility model discloses do not destroy matrigel and cell culture plate of cell, be provided with the bottom plate, be provided with a plurality of culture hole and a plurality of operation groove on the bottom plate, culture hole and at least one be provided with the pellicle between the operation groove and pass through the pellicle communicates.

According to the utility model discloses cell culture plate of not destroying matrigel and cell has following technological effect at least: after the culture solution and the matrigel are added into the culture holes, the cells can be added and cultured. Because be provided with the pellicle and communicate through the pellicle between culture hole on the bottom plate and at least one operation groove, and the pellicle can select for use and can see through the culture solution, but does not see through the pellicle of cell and matrigel. When culture solution nutrient in the culture hole is not enough or other reasons need to be changed culture solution, at first, the rifle head part with liquid suction device stretches into the operation inslot that corresponds with the culture hole, and then when liquid suction device's rifle head absorbs the culture solution in the operation inslot, the downthehole original culture solution of culture can pass the pellicle and enter into the operation inslot, thereby absorb by liquid suction device, after the downthehole original culture solution of culture is absorbed, liquid suction device's rifle head stretches into the operation inslot and adds the operation inslot with new culture solution, new culture solution can pass the pellicle and enter into corresponding culture downthehole, thereby realize the change of culture solution. Because imbibition device and liquid feeding device all operate in the operation inslot when changing the culture solution, and the pellicle selection only can see through the culture solution, but does not see through cell and matrigel, and then the rifle head of imbibition device and liquid feeding device can not touch cell and matrigel to can not lead to the fact destruction to cell and matrigel, reduced the probability of experiment failure, simplified the experiment degree of difficulty, saved the experimental time.

According to some embodiments of the invention, the number of culture wells is multiple.

According to some embodiments of the invention, the culture wells are arranged in an array on the base plate.

According to some embodiments of the present invention, the operation grooves correspond to the culture wells in the same number one to one.

According to some embodiments of the utility model, the operation groove sets up in corresponding one side of cultivateing the hole.

According to some embodiments of the invention, the semi-permeable membrane is arranged between the lower end of the operating bath and the lower end of the culture well.

According to some embodiments of the utility model, the bottom of pellicle with correspond cultivate the bottom parallel and level in hole, the bottom in operation groove is less than corresponding cultivate the bottom in hole.

According to some embodiments of the invention, the surface of the semi-permeable membrane is a spherical surface.

According to some embodiments of the invention, the semi-permeable membrane is a membrane of molecular material.

According to some embodiments of the present invention, the semi-permeable membrane has a plurality of filtering holes, and the filtering holes have a pore size of 100nm to 10 μm.

According to some embodiments of the invention, the cell culture plate is further provided with a cover plate for covering the bottom plate.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view showing a connection between a culture well and one of operation wells;

FIG. 3 is a schematic view showing another connection between a culture well and an operation well;

fig. 4 is a schematic view of a partial structure of the present invention;

reference numerals:

a bottom plate 100, a culture well 200, a manipulation groove 300, a semi-permeable membrane 400, and a cover plate 500.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention. Furthermore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

A cell culture plate that does not destroy matrigel and cells according to an embodiment of the present invention is described below with reference to fig. 1 to 4.

According to the utility model discloses cell culture plate of not destroying matrigel and cell, as shown in fig. 1 to 4, be provided with bottom plate 100, be provided with a plurality of culture hole 200 and a plurality of operation groove 300 on the bottom plate 100, be provided with pellicle 400 and communicate through pellicle 400 between culture hole 200 and at least one operation groove 300.

In this embodiment, after the culture medium and the matrigel are added to the culture well 200, the cells are added and cultured. Since the culture hole 200 on the bottom plate 100 is provided with the semipermeable membrane 400 and communicated with the at least one operation tank 300 through the semipermeable membrane 400, the semipermeable membrane 400 can be a semipermeable membrane 400 which can permeate the culture solution but does not permeate the cells and the matrigel. When nutrient of the culture solution in the culture hole 200 is not enough and the culture solution needs to be replaced, firstly, the gun head part of the liquid absorbing device extends into the operation groove 300 corresponding to the culture hole 200, and then when the gun head of the liquid absorbing device absorbs the culture solution in the operation groove 300, the original culture solution in the culture hole 200 can penetrate through the semipermeable membrane 400 and enter into the operation groove 300, so that the culture solution is absorbed by the liquid absorbing device, after the original culture solution in the culture hole 200 is approximately absorbed, the gun head of the liquid absorbing device extends into the operation groove 300 and adds new culture solution into the operation groove 300, the new culture solution can penetrate through the semipermeable membrane 400 and enter into the corresponding culture hole 200, and therefore the replacement of the culture solution is realized. Because imbibition device and liquid feeding device all operate in operation groove 300 when changing the culture solution, and pellicle 400 only can see through the culture solution, nevertheless do not see through cell and matrigel, and then the rifle head of imbibition device and liquid feeding device can not touch cell and matrigel to can not lead to the fact destruction to cell and matrigel, reduced the probability of experiment failure, simplified the experiment degree of difficulty, saved the experimental time.

It should be noted that the number of the culture wells 200 may be one or two or more, and the number of the operation grooves 300 may be one or two or more, according to actual requirements. In this embodiment, the semi-permeable membrane 400 is disposed between the culture well 200 and at least one operation tank 300, and may be two or more operation tanks 300 corresponding to one culture well 200 and the semi-permeable membrane 400 is disposed between two or more operation tanks 300 corresponding to one culture well 200, for example, when the pore size of the culture well 200 is large, two or more operation tanks 300 may be disposed on the periphery of the culture well 200, and then the culture solution in each region of the culture well 200 can be rapidly replaced by using two or more operation tanks 300, thereby avoiding the problem that the culture solution is too slowly diffused due to the excessively large pore size of the culture well 200; in addition, when the types of cells cultured in the culture wells 200 are consistent, two or more culture wells 200 may be correspondingly arranged in one operation tank 300, and a semi-permeable membrane 400 is arranged between the two or more culture wells 200, so that the two or more culture wells 200 can be simultaneously replaced by adding and taking liquid in the one operation tank 300, which is more convenient to operate and saves more time and labor; furthermore, one operation well 300 may be provided corresponding to one culture well 200, and a semi-permeable membrane 400 may be provided between the operation well 300 and the operation well. The imbibition device can be an ear-sucking ball or a liquid transfer device, the liquid feeding device can also be an ear-sucking ball or a liquid transfer device, the ear-sucking ball or the liquid transfer device is a common tool for replacing culture solution in cell culture, and the structure, the working principle and the working process are not repeated. A semi-permeable membrane is a membrane that allows only certain molecules or ions to diffuse in and out, i.e. a membrane that is selective to the passage of different particles. Such as cell membranes, bladder membranes, parchment, and man-made membranes of molecular materials, and the like, and, in addition, modern technology makes semipermeable membranes by precipitating appropriate compounds (e.g., copper ferricyanide) into the pores of porous walls (e.g., unglazed ceramics). The semipermeable membrane used in this example may be parchment paper or a membrane of artificially prepared molecular material. It is understood that there are various positional relationships between the operation well 300 and the corresponding culture well 200, for example, as shown in FIG. 2, the operation well 300 may be positioned in the corresponding culture well 200 with the outer sidewall of one side of the operation well 300 being attached to the inner sidewall of one side of the culture well 200, the sidewall of the other side of the operation well 300 being positioned in the culture well 200, and the semi-permeable membrane 400 being disposed on the sidewall of the other side of the operation well 300. Further, as shown in FIG. 3, the operation well 300 may be located on one side of the corresponding culture well 200. In addition, the operation slot 300 can also be located in the corresponding culture hole 200 near the middle position, i.e. the culture hole 200 is sleeved in the corresponding operation slot 300, so that the side walls of the operation slot 300 in multiple directions can be provided with the semipermeable membrane 400, and the efficiency is higher when the culture solution is replaced. The culture wells 200 may be circular wells, square wells or other suitable shapes, the operation grooves 300 may be circular wells, square wells or other suitable shapes, and when there are two or more culture wells 200, the sizes of the culture wells 200 may be all the same or partially the same or different according to actual needs.

In some embodiments of the present invention, as shown in fig. 1, the number of culture wells 200 is plural. And further more cells and/or more kinds of cells can be cultured simultaneously, so that the experiment time is saved, and the experiment efficiency is improved. It is understood that the number of culture wells 200 may be three or more.

In some embodiments of the present invention, the culture wells 200 are arranged in an array on the base plate 100. As shown in FIG. 1, the culture wells 200 may be arranged in five rows on the bottom plate 100, and the number of the culture wells 200 in each row is five. The culture holes 200 are arranged in an array, so that the arrangement of the culture holes 200 is more reasonable, the occupied area is less, the size required by the bottom plate 100 is smaller, the material is saved, and the occupied space of the bottom plate 100 is reduced. In addition, cultivate hole 200 and be array arrangement for the experiment operator is difficult to cause when the record experiment data to obscure, thereby can reduce the probability that the error appears in the experiment.

In some embodiments of the present invention, the operation slots 300 are the same in number and correspond to the culture wells 200 one by one. That is, one cultivation hole 200 corresponds and sets up an operation groove 300 and be provided with pellicle 400 between this operation groove 300, and then every cultivation hole 200 all carries out the change operation of culture solution through an operation groove 300 that corresponds, not only makes to change more accurately when changing the culture solution, thereby can improve the cultivation effect of cell, can avoid appearing cross infection between each cultivation hole 200 moreover, thereby can reduce the probability that the cell culture experiment appears failure.

In some embodiments of the present invention, the semi-permeable membrane 400 is disposed between the lower end of the operation tank 300 and the lower end of the culture well 200. A through hole or a slit may be provided between the lower end of the operation well 300 and the lower end of the culture well 200, and a semi-permeable membrane 400 may be provided on the through hole or the slit. The semipermeable membrane 400 is disposed between the lower end of the operation tank 300 and the lower end of the culture hole 200, and when the culture solution is replaced, the original culture solution in the culture hole 200 sequentially enters the operation tank 300 from the lower end thereof, so that the original culture solution in the culture hole 200 can be replaced to a greater extent, and the culture effect of the cells is good.

In some embodiments of the present invention, as shown in fig. 4, the bottom end of the semi-permeable membrane 400 is flush with the bottom end of the corresponding culture well 200 and is connected to the bottom end of the corresponding culture well 200, and the bottom end of the operation tank 300 is lower than the bottom end of the corresponding culture well 200. Furthermore, when the culture medium is replaced, the original culture medium in the culture well 200 can be maximally inserted into the corresponding operation tank 300, so that the culture medium can be maximally replaced, and the cell culture effect is improved.

In some embodiments of the present invention, the surface of the semipermeable membrane 400 is a spherical surface. One of them surface of pellicle 400 is concave sphere promptly, and another surface correspondence sets up to convex sphere, compares in pellicle 400 for flat structure, and pellicle 400's in this embodiment filter area is bigger, and then filtration efficiency is higher to make the change culture solution required time shorter, saved the experimental time.

In some embodiments of the present invention, the semipermeable membrane 400 is a membrane of molecular material. The molecular material membrane has low manufacturing cost, low adsorptivity, high filtration efficiency, environmental protection and no pollution. The membrane of molecular material may be a polycarbonate membrane or a cellulose acetate membrane.

In some embodiments of the present invention, the semi-permeable membrane 400 has a plurality of filtering holes, and the size of the filtering holes is 100nm to 10 μm. When the aperture size of the filter holes is 100 nm-10 μm, the filter holes can just penetrate through the water and the culture medium in the culture solution, but cannot penetrate through the cells and the matrigel, so that the filter effect of the semipermeable membrane 400 is good, and the cells and the matrigel can be prevented from entering the corresponding operation tank 300 from the culture holes 200, thereby avoiding the cells and the matrigel from being damaged.

In some embodiments of the present invention, as shown in fig. 1, the cell culture plate is further provided with a cover plate 500 for covering the bottom plate 100. The cover plate 500 is adapted to the bottom plate 100 in size, so that the cover plate 500 can cover the bottom plate 100, and the culture solution, matrigel and cells in the culture holes 200 and the culture solution in the operation tank 300 are prevented from being polluted by the outside in the process of culturing cells.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples" or the like mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. The utility model provides a do not destroy matrigel and cell culture board of cell, its characterized in that is provided with the bottom plate, be provided with a plurality of culture hole and a plurality of operation groove on the bottom plate, cultivate hole and at least one be provided with the pellicle between the operation groove and pass through the pellicle communicates, the pellicle set up in the lower extreme of operation groove with between the lower extreme of cultivation hole, the bottom of pellicle with correspond the bottom parallel and level of cultivation hole, the bottom of operation groove is less than corresponding the bottom of cultivation hole, the surface of pellicle is the sphere.

2. The cell culture plate that does not damage matrigel and cells according to claim 1, wherein the number of the culture wells is plural.

3. A cell culture plate that does not disrupt matrigel and cells according to claim 2, wherein the culture wells are arranged in an array on the bottom plate.

4. A cell culture plate without damaging matrigel and cells according to claim 3, wherein the operation wells are the same in number as the culture wells and correspond one to one.

5. A cell culture plate without damaging matrigel and cells according to any one of claims 1 to 4, wherein the semi-permeable membrane is a membrane of molecular material.

6. A cell culture plate without damaging matrigel and cells according to any one of claims 1 to 4, wherein the semi-permeable membrane has a plurality of filtration pores with a pore size of 100nm to 10 μm.

7. A cell culture plate without damaging matrigel and cells according to any one of claims 1 to 4, wherein the cell culture plate is further provided with a cover plate for covering the bottom plate.