CN214032445U

CN214032445U - Saliva sample nucleic acid purifier

Info

Publication number: CN214032445U
Application number: CN202022522908.2U
Authority: CN
Inventors: 黄震
Original assignee: Sentinall Biotechnologies Inc
Current assignee: Sentinall Biotechnologies Inc
Priority date: 2020-11-04
Filing date: 2020-11-04
Publication date: 2021-08-24
Anticipated expiration: 2030-11-04

Abstract

The utility model discloses a saliva sample nucleic acid purifier, which comprises a nucleic acid purifying column and a waste liquid tube; a filter pipe is arranged around the side surface of the waste liquid pipe; the waste liquid pipe is sleeved in the filter pipe; the nucleic acid purification column is connected with the waste liquid tube through a locking device, and the waste liquid tube is connected with the filter tube through an embedding device; the nucleic acid purification column is symmetrically provided with purification fixing rods, and the waste liquid pipe is provided with a purification accommodating groove; the waste liquid fixing rods are symmetrically arranged on the waste liquid pipe, and the filtering pipe is provided with a waste liquid fixing and accommodating groove; a filter layer is arranged between the waste liquid tube nucleic acid purification columns; the bottom of the filter pipe is provided with a liquid seal device; the utility model has the advantages that: the purification fixing rod and the purification container, the waste liquid fixing rod and the waste liquid fixing containing tank are used for fixing the sealing cover by adopting the threaded connecting ring, and then the purification and the filtration which are stable and rapid and prevent the backflow are carried out by the locking device, the embedding device and the liquid sealing device; the material leakage holes are arranged in a gradually reduced manner through the pore diameter, and the filtering is rapid.

Description

Saliva sample nucleic acid purifier

Technical Field

The utility model relates to the field of medical equipment, specifically a saliva sample nucleic acid purifier.

Background

In recent years, with the generation of a large amount of delicate foods and poor living habits, the traditional "metabolic diseases" of the elderly gradually become younger, such as: 1. diabetes, 2. diabetic ketoacidosis, 3. hyperglycemic hyperosmolar syndrome, 4. hypoglycemia, 5. gout, 6. protein-energy dystrophy, 7. vitamin a deficiency, 8. scurvy, 9. vitamin D deficiency, 10. osteoporosis, etc. Among the most convenient and rapid methods of detection, i.e., collection of saliva samples for analysis, the more recent of the existing methods of analysis, described by the authors of WO 01/78652 as "metabolomics", is a technique that uses small molecule profiles obtained by a variety of analyses, including spectroscopic and chromatographic analyses, while emphasizing the use of the entire profile rather than individual chemical signatures for diagnosing and predicting disease states, predicting individual response to therapeutic agents, and for monitoring the efficacy of therapeutic agents in clinical trials.

In the past few years, the use of a technique "metabolomics" involving multivariate analysis of spectroscopic data to assess disease status has also received much attention, particularly from Nicholson and co-workers thereof. For example, WO02/086478 provides a detailed disclosure of spectroscopic analysis, in particular the principal component analysis of 1H nuclear magnetic resonance spectroscopy and its use as a diagnostic technique. The publication discloses a long list of diseases to which the technique may be applied, including dental diseases such as caries, gum diseases and gingivitis. The publication also discloses a number of fluid sample types, in particular saliva, to which the technique can be applied. While WO 03/107270 is based on a metabolomic approach for metabolic phenotype of subjects. This patent application describes in particular the following applications of metabolomics: predicting response to dosing, selecting a collection of subjects with similar phenotypes, and for easy biomarker identification. WO 2004/038602 further describes a general technique for data acquisition in connection with metabolomic datasets. US 2007/0043518 (Nicholson et al) is detailed in terms of statistical analysis that can be performed on metabolomic datasets and their use for identifying components of complex systems, such as identifying biomarkers in biological fluids.

In particular, in the above-mentioned biomarkers in the molecular biology field, it is often necessary to extract macromolecular substances such as DNA and RNA from a biological sample and separate and purify these macromolecular substances, and the efficient separation and purification of nucleic acid substances becomes a key technology in the molecular biology field.

The existing general process of nucleic acid purification is to destroy the structure of organism (mainly cell structure), remove components (mainly cell debris and protein) except nucleic acid, further purify the primarily separated nucleic acid, and separate and extract nucleic acid from biological sample by using nucleic acid purification column, which is a common means in molecular biology field, the general nucleic acid purification column is a circular tubular structure, comprising a circular tube column, one end of the column is provided with a sealing cover, the other end is filled with a porous sieve plate or membrane, and a material capable of adsorbing biological substances such as nucleic acid is added on the porous sieve plate.

In the existing nucleic acid purification kit on the market, taking a plasmid DNA purification kit as an example, high-speed (more than or equal to 12000 g) centrifugation is needed for 10 minutes to precipitate bacterial debris and protein, and then supernatant is added into a nucleic acid purification column to separate and purify plasmid DNA. Although the existing equipment with unified filtration and purification is available, most of the equipment adopts a buckle form for fixation and a multi-tube sleeving form, and in the centrifugation stage, when the liquid to be filtered in the nucleic acid purification column is too much and the waste liquid filtered in the peripheral centrifugal tube is too much, the liquid is very easy to flow back, the adsorbed nucleic acid is polluted, the sample is wasted, and the detection accuracy is influenced; meanwhile, the sleeve is adopted, so that vibration is easy to occur during splitting, unstable nucleic acid overflows again, and a sample is wasted.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art's not enough, provide a purification and filterable nucleic acid purification device stable quick, that prevent to kick back.

The purpose of the utility model is realized through the following technical scheme:

a saliva sample nucleic acid purifier including a nucleic acid purification cartridge and a waste liquid tube accommodating the nucleic acid purification cartridge; a filter pipe is arranged on the side surface of the waste liquid pipe in a surrounding manner; the waste liquid pipe is sleeved in the filter pipe; the nucleic acid purification column is fixedly connected with the side surface of the waste liquid tube through a locking device, and the corresponding waste liquid tube is fixedly connected with the side surface of the filter tube through an embedding device;

the liquid inlet end of the nucleic acid purification column, the upper opening end of the waste liquid pipe and the upper opening end of the filter pipe are arranged on the same horizontal plane; purification fixing rods are arranged on two sides of the liquid inlet end of the nucleic acid purification column, and purification accommodating grooves for accommodating the purification fixing rods are arranged at the upper opening ends of the corresponding waste liquid tubes; the purification fixing rods are symmetrically arranged relative to the vertical plane where the axis of the nucleic acid purification column is located; waste liquid fixing rods are arranged on two sides of the upper opening end of the waste liquid pipe, and a waste liquid fixing and accommodating groove for accommodating the waste liquid fixing rods is formed in the corresponding upper opening end of the filter pipe; the waste liquid fixing rods are symmetrically arranged relative to the vertical plane where the axis of the waste liquid pipe is located; a threaded connecting ring is fixed at the upper opening end of the filter tube, and an inlet and outlet groove for the waste liquid fixing rod to enter and exit is formed in each of two sides of the threaded connecting ring;

a sealing cover is fixed above the filtering pipe through the threaded connecting ring; a filter layer is arranged between the waste liquid tube and the nucleic acid purification column; one end of the filter layer is connected with the liquid outlet end of the nucleic acid purification column in an abutting mode, and the other end of the filter layer is connected with the lower opening end of the waste liquid tube in an abutting mode;

the bottom of the filter pipe is also provided with a liquid seal device which is fixed at the liquid outlet end of the waste liquid pipe and is abutted against the inner wall of the filter pipe.

Preferably, in order to further achieve the purpose of stable purification, the locking device comprises a locking round block, a locking telescopic rod, a locking spring and a locking accommodating groove; the locking accommodating groove is fixed on the outer side surface of the nucleic acid purification column, the bottom surface of the locking accommodating groove is fixed with the locking telescopic rod, one end of the locking telescopic rod, which is far away from the locking accommodating groove, is fixed with the locking round block, the side surface of the locking telescopic rod is surrounded with a locking spring, one end of the locking spring is fixed on the locking accommodating groove, and the other end of the locking spring is fixed on the bottom surface of the locking round block; the locking round block is abutted against the inner wall of the waste liquid pipe and is symmetrically arranged relative to the vertical plane where the axis of the nucleic acid purification column is located; the inner wall of the waste liquid pipe is provided with a locking arc-shaped groove for accommodating the locking round block; through adopting including the locking circle piece, the locking telescopic link, the locking device of locking spring and locking holding tank, the locking circle piece of the side of nucleic acid purification post receives the extrusion of waste liquid pipe inner wall, thereby extrusion locking telescopic link, and then extrusion locking spring, thereby make the locking circle piece crowd in the locking holding tank, when the locking circle piece slides in the locking arc wall of waste liquid pipe, the locking circle piece does not receive the extrusion this moment, thereby the locking spring extension, drive the extension of locking telescopic link, thereby make the locking circle piece fix the locking arc wall in, and then realize the locking of the waste liquid pipe of nucleic acid purification post, and then cooperation purification dead lever, realize the stability in the purification stage of nucleic acid purification post.

Preferably, in order to further achieve the purpose of stabilizing the filtration, the insertion device includes an insertion receiving groove, an insertion fixing rod, and an insertion spring; the embedded accommodating groove is fixed on the outer side surface of the waste liquid pipe, the embedded spring is arranged at the center of the embedded accommodating groove, and an embedded fixing rod is fixed at one end of the embedded spring, which is far away from the embedded accommodating groove; the embedded fixing rod is connected in the embedded accommodating groove in a sliding manner; the inner wall of the filter pipe is provided with an embedded annular groove for accommodating the embedded fixing rod; the embedded annular groove is fixed at the center of the inner wall of the filter pipe; through the embedding device including the embedding holding tank, embedding dead lever and embedding spring, the embedding dead lever of the side when the waste liquid pipe receives the extrusion of the inner wall of filter tube, thereby make embedding dead lever atress extrusion embedding spring, the embedding spring shrink, make embedding dead lever shrink get into in the embedding holding tank, when the embedding dead lever moves the embedding ring channel of filter tube, because the embedding dead lever does not receive pressure this moment, consequently, the embedding spring extension, drive the embedding dead lever extension, and then realize the embedding dead lever embedding in the embedding ring channel, and then cooperate the waste liquid dead lever, realize fixing between waste liquid pipe and the filter tube, realize filtering stage's stability.

Preferably, in order to further realize stable and rapid purification and filtration, the liquid outlet end of the nucleic acid purification column is provided with a material leakage ball; a plurality of material leaking holes are formed in the material leaking ball, and the material leaking ball is fixed right above the filter layer; a plurality of material leaking holes are formed in the material leaking ball; the aperture of the material leaking hole is gradually reduced along the flowing direction of the liquid; be the weeping hole that reduces the setting gradually through the aperture, the sample in the nucleic acid purification post flows with higher speed, makes the liquid after the purification can stabilize quick flow waste liquid pipe in, and impurity can be quick gets into the filtration stage through the weeping hole.

Preferably, in order to further achieve the purpose of preventing backflow, the liquid sealing device comprises a liquid sealing floating plate, an upper magnetic suction block and a lower magnetic suction block; the upper magnetic blocks are arranged on two sides of the liquid outlet end of the waste liquid pipe and are symmetrically arranged relative to the vertical plane where the axis of the waste liquid pipe is positioned; the lower magnetic attraction block is fixed on the lower surface of the liquid seal floating plate and is arranged corresponding to the upper magnetic attraction block; the liquid seal floating plate is abutted against the inner wall of the filter pipe and is arranged at the bottom end of the filter pipe; the liquid seal floating plate is provided with a plurality of liquid permeating holes which are uniformly distributed on the circumference, and the center of the liquid seal floating plate is surrounded into a seal circular plate; the diameter of the sealing circular plate is the same as that of the liquid outlet end of the waste liquid pipe; through adopting the liquid seal device including liquid seal kickboard, go up magnetism piece and magnetism piece down, utilize the counterpoint design of magnetism piece and magnetism piece down of inhaling, and the design of the liquid hole and sealed plectane on the liquid seal kickboard, thereby make the waste liquid after the filtration leak the below of sealed plectane through liquid hole, make sealed plectane increase and come up along with the solution volume, when outlying filter tube collection volume was full, the magnetism piece and last magnetism piece of inhaling down of the lower surface of liquid seal kickboard adsorb each other, thereby make the play liquid end of sealed plectane seal filter tube, thereby prevent the backsurge phenomenon that the too big result in of filtration volume, guarantee the safety of the nucleic acid sample of collection.

The utility model has the advantages that:

1. the purification fixing rods at the liquid inlet end of the nucleic acid purification column are matched with the purification containing groove at the upper opening end of the waste liquid pipe, the waste liquid fixing rods at two sides of the upper opening end of the waste liquid pipe are matched with the waste liquid fixing containing groove at the upper opening end of the filter pipe, and meanwhile, the sealing cover fixed by the threaded connection ring is adopted, so that the upper ends of the nucleic acid purification column, the waste liquid pipe and the filter pipe are fixed, and then the nucleic acid purification column and the waste liquid pipe are respectively fixed in a locking manner and embedded and fixed between the waste liquid pipe and the filter pipe through a locking device and an embedding device, so that the purification and the filtration between the nucleic acid purification column and the filter pipe can stably work; and then the excessive sealing of the filtered liquid is carried out through a liquid sealing device, so that the back-flush phenomenon is prevented, and the safety of the nucleic acid sample is ensured.

2. Through adopting including the locking circle piece, the locking telescopic link, the locking device of locking spring and locking holding tank, the locking circle piece of the side of nucleic acid purification post receives the extrusion of waste liquid pipe inner wall, thereby extrusion locking telescopic link, and then extrusion locking spring, thereby make the locking circle piece crowd in the locking holding tank, when the locking circle piece slides in the locking arc wall of waste liquid pipe, the locking circle piece does not receive the extrusion this moment, thereby the locking spring extension, drive the extension of locking telescopic link, thereby make the locking circle piece fix the locking arc wall in, and then realize the locking of the waste liquid pipe of nucleic acid purification post, and then cooperation purification dead lever, realize the stability in the purification stage of nucleic acid purification post.

3. Through the embedding device including the embedding holding tank, embedding dead lever and embedding spring, the embedding dead lever of the side when the waste liquid pipe receives the extrusion of the inner wall of filter tube, thereby make embedding dead lever atress extrusion embedding spring, the embedding spring shrink, make embedding dead lever shrink get into in the embedding holding tank, when the embedding dead lever moves the embedding ring channel of filter tube, because the embedding dead lever does not receive pressure this moment, consequently, the embedding spring extension, drive the embedding dead lever extension, and then realize the embedding dead lever embedding in the embedding ring channel, and then cooperate the waste liquid dead lever, realize fixing between waste liquid pipe and the filter tube, realize filtering stage's stability.

4. Be the weeping hole that reduces the setting gradually through the aperture, the sample in the nucleic acid purification post flows with higher speed, makes the liquid after the purification can stabilize quick flow waste liquid pipe in, and impurity can be quick gets into the filtration stage through the weeping hole.

5. Through adopting the liquid seal device including liquid seal kickboard, go up magnetism piece and magnetism piece down, utilize the counterpoint design of magnetism piece and magnetism piece down of inhaling, and the design of the liquid hole and sealed plectane on the liquid seal kickboard, thereby make the waste liquid after the filtration leak the below of sealed plectane through liquid hole, make sealed plectane increase and come up along with the solution volume, when outlying filter tube collection volume was full, the magnetism piece and last magnetism piece of inhaling down of the lower surface of liquid seal kickboard adsorb each other, thereby make the play liquid end of sealed plectane seal filter tube, thereby prevent the backsurge phenomenon that the too big result in of filtration volume, guarantee the safety of the nucleic acid sample of collection.

Drawings

FIG. 1 is a schematic view of the apparatus of the present invention;

fig. 2 is a schematic view of the locking device of the present invention;

FIG. 3 is a schematic view of an embedment device of the present invention;

in the figure, 1-nucleic acid purification column, 11-purification fixing rod, 12-leakage ball, 121-leakage hole, 2-waste liquid tube, 21-purification receiving tank, 22-waste liquid fixing rod, 23-filter layer, 24-locking arc tank, 3-filter tube, 31-waste liquid fixing receiving tank, 32-threaded connection ring, 33-embedded annular groove, 4-locking device, 41-locking round block, 42-locking telescopic rod, 43-locking spring, 44-locking receiving tank, 5-embedding device, 51-embedded receiving tank, 52-embedded fixing rod, 53-embedded spring, 6-sealing cover, 7-liquid sealing device, 71-liquid sealing floating plate, 72-upper magnetic block, 73-lower magnetic block.

Detailed Description

The technical solution of the present invention is described in further detail below with reference to the accompanying drawings, but the scope of the present invention is not limited to the following description.

The inner wall of the nucleic acid purification column 1, the surface of the leakage ball 12 and the leakage hole 121 are provided with nucleic acid-adsorbing porous membranes, such as silica gel membrane, glass fiber membrane, silica nano material, graphene, titanium dioxide crystal film, etc., which are acid-base resistant and can specifically adsorb nucleic acid

As shown in FIG. 1, a saliva sample nucleic acid purifier includes a nucleic acid purification cartridge 1 and a waste liquid tube 2 accommodating the nucleic acid purification cartridge 1; a filter pipe 3 is arranged on the side surface of the waste liquid pipe 2 in a surrounding way; the waste liquid pipe 2 is sleeved in the filter pipe 3; the nucleic acid purification column 1 is fixedly connected with the side surface of the waste liquid tube 2 through a locking device 4, and the corresponding waste liquid tube 2 is fixedly connected with the side surface of the filter tube 3 through an embedding device 5;

the liquid inlet end of the nucleic acid purification column 1, the upper opening end of the waste liquid tube 2 and the upper opening end of the filter tube 3 are arranged on the same horizontal plane; purification fixing rods 11 are arranged on two sides of the liquid inlet end of the nucleic acid purification column 1, and purification accommodating grooves 21 for accommodating the purification fixing rods 11 are arranged at the upper opening ends of the corresponding waste liquid tubes 2; the purification fixing rods 11 are symmetrically arranged relative to the vertical plane where the axis of the nucleic acid purification column 1 is located; two sides of the upper opening end of the waste liquid pipe 2 are provided with waste liquid fixing rods 22, and the corresponding upper opening end of the filter pipe 3 is provided with a waste liquid fixing accommodating groove 31 for accommodating the waste liquid fixing rods 22; the waste liquid fixing rods 22 are symmetrically arranged relative to the vertical plane where the axis of the waste liquid pipe 2 is located; a threaded connecting ring 32 is fixed at the upper opening end of the filter tube 3, and an inlet and outlet groove 321 for the waste liquid fixing rod 22 to enter and exit is formed in the two sides of the threaded connecting ring 32;

a sealing cover 6 is fixed above the filter pipe 3 through the threaded connecting ring 32; a filter layer 23 is arranged between the waste liquid tube 2 and the nucleic acid purification column 1; one end of the filter layer 23 is connected with the liquid outlet end of the nucleic acid purification column 1, and the other end is connected with the lower opening end of the waste liquid tube 2; the lower end opening of the waste liquid pipe 2 is arranged in a sharp mouth shape;

the bottom of the filtering pipe 3 is also provided with a liquid sealing device 7, and the liquid sealing device 7 is fixed at the liquid outlet end of the waste liquid pipe 2 and is abutted against the inner wall of the filtering pipe 3.

As shown in fig. 2, for the purpose of further achieving stable purification, the locking device 4 includes a locking round block 41, a locking telescopic rod 42, a locking spring 43 and a locking receiving groove 44; the locking containing groove 44 is fixed on the outer side surface of the nucleic acid purification column 1, the locking telescopic rod 42 is fixed on the bottom surface of the locking containing groove 44, the locking round block 41 is fixed on one end of the locking telescopic rod 42 far away from the locking containing groove 44, the locking spring 43 is enclosed on the side surface of the locking telescopic rod 42, one end of the locking spring 43 is fixed on the locking containing groove 44, and the other end is fixed on the bottom surface of the locking round block 41; the locking round block 41 is abutted against the inner wall of the waste liquid tube 2 and is symmetrically arranged relative to the vertical plane where the axis of the nucleic acid purification column 1 is located; a locking arc-shaped groove 24 for accommodating the locking round block 41 is arranged on the inner wall of the waste liquid pipe 2; through adopting locking device 4 including locking circle piece 41, locking telescopic link 42, locking spring 43 and locking holding tank 44, the locking circle piece 41 of the side of nucleic acid purification post 1 receives the extrusion of waste liquid pipe 2 inner wall, thereby extrude locking telescopic link 42, and then extrude locking spring 43, thereby make locking circle piece 41 crowd in the locking holding tank 44, when locking circle piece 41 slides in the locking arc wall 24 of waste liquid pipe 2, locking circle piece 41 does not receive the extrusion this moment, thereby locking spring 43 extends, drive locking telescopic link 42 extension, thereby make locking circle piece 41 fix in locking arc wall 24, and then realize the locking of waste liquid pipe 2 of nucleic acid purification post 1, and then cooperation purification dead lever 11, realize the stability of the purification stage of nucleic acid purification post 1.

As shown in fig. 3, in order to further achieve the purpose of stabilizing the filtration, the inserting device 5 includes an inserting receiving groove 51, an inserting fixing rod 52 and an inserting spring 53; the insertion accommodating groove 51 is fixed on the outer side surface of the waste liquid pipe 2, the insertion spring 53 is arranged at the center of the insertion accommodating groove 51, and an insertion fixing rod 52 is fixed at one end of the insertion spring 53 far away from the insertion accommodating groove 51; the embedding fixing rod 52 is connected in the embedding accommodating groove 51 in a sliding manner; the inner wall of the filter pipe 3 is provided with an embedded annular groove 33 for accommodating the embedded fixing rod 52; the embedded annular groove 33 is fixed at the center of the inner wall of the filter pipe 3; through embedding device 5 including embedding holding tank 51, embedding dead lever 52 and embedding spring 53, embedding dead lever 52 when the side of waste liquid pipe 2 receives the extrusion of the inner wall of filter tube 3, thereby make embedding dead lever 52 atress extrusion embedding spring 53, embedding spring 53 contracts, make embedding dead lever 52 contract and get into in embedding holding tank 51, when embedding dead lever 52 moves to the embedding ring channel 33 of filter tube 3 in, because embedding dead lever 52 does not receive pressure this moment, consequently embedding spring 53 extends, drive embedding dead lever 52 extension, and then realize embedding dead lever 52 embedding in embedding ring channel 33, and then cooperation waste liquid dead lever 22, realize the fixed between waste liquid pipe 2 and the filter tube 3, realize the stability in filtering stage.

In order to further realize stable and rapid purification and filtration, the liquid outlet end of the nucleic acid purification column 1 is provided with a material leakage ball 12; a plurality of material leaking holes 121 are formed in the material leaking ball 12; the material leakage balls 12 are fixed right above the filter layer 23; the aperture of the material leaking hole 121 is gradually reduced along the flowing direction of the liquid; be the hourglass material hole 121 that reduces the setting gradually through the aperture, accelerate the sample flow in the nucleic acid purification post 1, make the liquid after the purification can stabilize in the quick inflow waste liquid pipe, and impurity can be quick gets into the filtration stage through hourglass material hole 121.

In order to further achieve the purpose of preventing backflow, the liquid sealing device 7 comprises a liquid sealing floating plate 71, an upper magnetic suction block 72 and a lower magnetic suction block 73; the upper magnetic blocks 72 are arranged on two sides of the liquid outlet end of the waste liquid pipe 2 and are symmetrically arranged relative to the vertical plane where the axis of the waste liquid pipe 2 is located; the lower magnetic attraction block 73 is fixed on the lower surface of the liquid seal floating plate 71 and is arranged corresponding to the upper magnetic attraction block 72; the liquid seal floating plate 71 is abutted against the inner wall of the filter pipe 3 and is arranged at the bottom end of the filter pipe 3; the liquid seal floating plate 71 is provided with a plurality of liquid permeating holes which are uniformly distributed on the circumference, and the center of the liquid seal floating plate 71 is surrounded to form a seal circular plate; the diameter of the sealing circular plate is the same as that of the liquid outlet end of the waste liquid pipe 2; by adopting the liquid sealing device 7 comprising the liquid sealing floating plate 71, the upper magnetic suction block 72 and the lower magnetic suction block 73, the alignment design of the upper magnetic suction block 72 and the lower magnetic suction block 73 and the design of the liquid penetrating hole and the sealing circular plate on the liquid sealing floating plate 71 are utilized, so that the filtered waste liquid leaks to the lower part of the sealing circular plate through the liquid penetrating hole, the sealing circular plate floats upwards along with the increase of the solution amount, when the collection amount of the peripheral filter tube 3 is full, the lower magnetic suction block 73 and the upper magnetic suction block 72 on the lower surface of the liquid sealing floating plate 71 are mutually adsorbed, so that the liquid outlet end of the filter tube 2 is sealed by the sealing circular plate, the backflow phenomenon caused by the overlarge filtration amount is prevented, and the safety of the collected nucleic acid sample is ensured.

Working process or principle: when nucleic acid purification is required, firstly, the inner wall of the waste liquid tube 2 is filled with the filter layer 23, then the purification fixing rod 11 of the nucleic acid purification column 1 is aligned with the purification accommodating groove 21 of the waste liquid tube 2, the nucleic acid purification column 1 is pushed into the waste liquid tube 2, at this time, in the locking device 4 comprising the locking round block 41, the locking telescopic rod 42, the locking spring 43 and the locking accommodating groove 44, the locking round block 41 on the side surface of the nucleic acid purification column 1 is extruded by the inner wall of the waste liquid tube 2, so as to extrude the locking telescopic rod 42 and further extrude the locking spring 43, so that the locking round block 41 is extruded into the locking accommodating groove 44, when the locking round block 41 slides into the locking arc-shaped groove 24 of the waste liquid tube 2, the locking round block 41 is not extruded at this time, so that the locking spring 43 extends, and drives the locking telescopic rod 42 to extend, so that the locking round block 41 is, further realizing the locking of the waste liquid tube 2 of the nucleic acid purification column 1, and further realizing the stable fixation of the nucleic acid purification column 1 by matching with the purification fixing rod 11;

at this time, the material leakage ball 12 with the material leakage hole 121 with gradually reduced aperture is abutted against the filter layer 23 in the waste liquid pipe 2; then, the waste liquid fixing rod 22 of the waste liquid tube 2 is aligned with the waste liquid fixing holding groove 31 of the filter tube 3, the waste liquid tube 2 with the nucleic acid purification cartridge 1 is pushed into the filter tube 3, the insertion device 5 including the insertion holding groove 51, the insertion fixing rod 52 and the insertion spring 53 at this time, and the insertion fixing rod 52 at the side of the waste liquid tube 2 is pressed by the inner wall of the filter tube 3, so that the insertion fixing rod 52 is pressed by the insertion spring 53, the insertion spring 53 is contracted, the insertion fixing rod 52 is contracted into the insertion holding groove 51, when the insertion fixing rod 52 moves into the insertion annular groove 33 of the filter tube 3, since the insertion fixing rod 52 is not pressed at this time, the insertion spring 53 is extended, the insertion fixing rod 52 is extended, and the insertion fixing rod 52 is inserted into the insertion annular groove 33, and the waste liquid fixing rod 22 is engaged, thereby fixing between the waste liquid tube 2 and the, the stabilization of the filtration stage is realized;

then, the nucleic acid sample is led into the nucleic acid purification column 1, the sealing cover 6 is tightly sealed through the threaded connection ring 32 of the filter tube 3, then the sample is centrifuged in the centrifuge, at the moment, after the nucleic acid adsorption porous membrane of the nucleic acid purification column 1 is completely adsorbed, the sample enters the filter layer 23 through the material leakage hole 121 of the material leakage ball 12 and is filtered, filtrate is collected through the filter tube 3, at the moment, filtered waste liquid leaks to the lower part of the sealing circular plate through the liquid permeation hole, so that the sealing circular plate floats upwards along with the increase of the solution amount, when the collection amount of the peripheral filter tube 3 is full, the lower magnetic suction block 73 and the upper magnetic suction block 72 on the lower surface of the liquid sealing floating plate 71 are mutually adsorbed, so that the sealing circular plate seals the liquid outlet end of the filter tube 2, thereby preventing the backflow phenomenon caused by the overlarge filtration amount, ensuring the safety of the collected nucleic acid sample, thereby completing the purification and filtration stage, after the work is completed, the waste liquid tube 2 is reversely disassembled according to the process and discarded, namely, the nucleic acid treatment of the purified and filtered saliva sample which is stable and quick and prevents the back flush is realized.

The foregoing is illustrative of the preferred embodiments of the present invention, and it is to be understood that the invention is not limited to the precise forms disclosed herein, and that various other combinations, modifications, and environments may be resorted to, falling within the scope of the invention as defined by the appended claims. But that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention, which is to be limited only by the claims appended hereto.

Claims

1. Saliva sample nucleic acid purifier, its characterized in that: comprises a nucleic acid purification column (1) and a waste liquid tube (2) accommodating the nucleic acid purification column (1); a filter pipe (3) is arranged around the side surface of the waste liquid pipe (2); the waste liquid pipe (2) is sleeved in the filter pipe (3); the nucleic acid purification column (1) is fixedly connected with the side surface of the waste liquid tube (2) through a locking device (4), and the corresponding waste liquid tube (2) is fixedly connected with the side surface of the filter tube (3) through an embedding device (5);

the liquid inlet end of the nucleic acid purification column (1), the upper opening end of the waste liquid pipe (2) and the upper opening end of the filter pipe (3) are arranged on the same horizontal plane; purification fixing rods (11) are arranged on two sides of the liquid inlet end of the nucleic acid purification column (1), and purification accommodating grooves (21) for accommodating the purification fixing rods (11) are formed in the upper opening ends of the corresponding waste liquid tubes (2); the purification fixing rods (11) are symmetrically arranged relative to the vertical plane where the axis of the nucleic acid purification column (1) is located; waste liquid fixing rods (22) are arranged on two sides of the upper opening end of the waste liquid pipe (2), and a waste liquid fixing accommodating groove (31) for accommodating the waste liquid fixing rods (22) is formed in the corresponding upper opening end of the filter pipe (3); the waste liquid fixing rods (22) are symmetrically arranged relative to the vertical plane where the axial lead of the waste liquid pipe (2) is located; a threaded connecting ring (32) is fixed at the upper opening end of the filter tube (3), and an inlet and outlet groove (321) for the waste liquid fixing rod (22) to enter and exit is formed in the two sides of the threaded connecting ring (32);

a sealing cover (6) is fixed above the filter pipe (3) through the threaded connecting ring (32); a filter layer (23) is arranged between the waste liquid tube (2) and the nucleic acid purification column (1); one end of the filter layer (23) is connected with the liquid outlet end of the nucleic acid purification column (1) in an abutting mode, and the other end of the filter layer is connected with the lower opening end of the waste liquid tube (2) in an abutting mode;

the bottom of the filtering pipe (3) is also provided with a liquid sealing device (7), and the liquid sealing device (7) is fixed at the liquid outlet end of the waste liquid pipe (2) and is abutted against the inner wall of the filtering pipe (3).

2. The saliva sample nucleic acid purifier of claim 1, wherein: the locking device (4) comprises a locking round block (41), a locking telescopic rod (42), a locking spring (43) and a locking accommodating groove (44); the locking accommodating groove (44) is fixed on the outer side surface of the nucleic acid purification column (1), the locking telescopic rod (42) is fixed on the bottom surface of the locking accommodating groove (44), the locking round block (41) is fixed on one end, far away from the locking accommodating groove (44), of the locking telescopic rod (42), a locking spring (43) is arranged on the side surface of the locking telescopic rod (42) in a surrounding mode, one end of the locking spring (43) is fixed on the locking accommodating groove (44), and the other end of the locking spring (43) is fixed on the bottom surface of the locking round block (41); the locking round block (41) is abutted against the inner wall of the waste liquid tube (2) and is symmetrically arranged relative to the vertical plane where the axis of the nucleic acid purification column (1) is located.

3. The saliva sample nucleic acid purifier of claim 2, wherein: the inner wall of the waste liquid pipe (2) is provided with a locking arc-shaped groove (24) for accommodating the locking round block (41).

4. The saliva sample nucleic acid purifier of any one of claims 1, 2 or 3, wherein: the embedding device (5) comprises an embedding accommodating groove (51), an embedding fixing rod (52) and an embedding spring (53); the embedding accommodating groove (51) is fixed on the outer side surface of the waste liquid pipe (2), the embedding spring (53) is arranged at the center of the embedding accommodating groove (51), and an embedding fixing rod (52) is fixed at one end, far away from the embedding accommodating groove (51), of the embedding spring (53); the embedding fixing rod (52) is connected in the embedding accommodating groove (51) in a sliding mode.

5. The saliva sample nucleic acid purifier of claim 4, wherein: the inner wall of the filter pipe (3) is provided with an embedded annular groove (33) for accommodating the embedded fixing rod (52); the embedded annular groove (33) is fixed at the center of the inner wall of the filter pipe (3).

6. The saliva sample nucleic acid purifier of claim 1, wherein: the liquid outlet end of the nucleic acid purification column (1) is provided with a material leakage ball (12); a plurality of material leaking holes (121) are formed in the material leaking ball (12), and the material leaking ball (12) is fixed right above the filter layer (23).

7. The saliva sample nucleic acid purifier of claim 6, wherein: the aperture of the material leaking hole (121) is gradually reduced along the flowing direction of the liquid.

8. The saliva sample nucleic acid purifier of claim 1, wherein: the liquid seal device (7) comprises a liquid seal floating plate (71), an upper magnetic suction block (72) and a lower magnetic suction block (73); the upper magnetic blocks (72) are arranged on two sides of the liquid outlet end of the waste liquid pipe (2) and are symmetrically arranged relative to the vertical plane where the axial lead of the waste liquid pipe (2) is positioned; the lower magnetic attraction block (73) is fixed on the lower surface of the liquid seal floating plate (71) and is arranged corresponding to the upper magnetic attraction block (72); the liquid seal floating plate (71) is abutted against the inner wall of the filter pipe (3) and is arranged at the bottom end of the filter pipe (3).

9. The saliva sample nucleic acid purifier of claim 8, wherein: the liquid seal floating plate (71) is provided with a plurality of liquid permeating holes which are uniformly distributed in the circumferential direction, and the center of the liquid seal floating plate (71) is surrounded to form a seal circular plate; the diameter of the sealing circular plate is the same as that of the liquid outlet end of the waste liquid pipe (2).