SUMMERY OF THE UTILITY MODEL
To foretell defect, the utility model aims at providing an automatic system of thawing of biological sample, it freezes the heating temperature and the time of depositing the pipe through two temperature sensor accurate control to pop out the freezing of the completion of thawing through playing the tubular construction fast and deposit the pipe, make through holding the structure tightly and freeze and deposit pipe and heating hole in close contact with, rapid heating up shortens the cell thawing time, reduces the cell death rate.
In order to achieve the above object, the utility model provides an automatic system of thawing of biological sample, including structure and the bullet tube structure of thawing.
The thawing structure comprises a heating block, wherein a heating hole for enabling the cryopreservation tube to be inserted is formed in the middle of the heating block, and the heating hole is formed in the vertical direction of the heating block.
The elastic tube structure comprises a lower pressing block, the top of the lower pressing block is abutted against the bottom of the cryopreservation tube, the lower portion of the lower pressing block is connected with an elastic assembly, an adsorption piece is correspondingly arranged at the bottom of the lower pressing block, and adsorption force exists between the adsorption piece and the lower pressing block under the preset condition.
According to the automatic biological sample unfreezing system, the heating blocks comprise a first heating block and a second heating block, the first heating block and the second heating block are symmetrically arranged, and semicircular heating arcs are arranged in the middle of the first heating block and the second heating block; the unfreezing structure is also provided with a holding assembly, and the holding assembly comprises support columns which are transversely arranged at the lower parts of the first heating block and the second heating block in a penetrating manner respectively; the bottom both sides of first heating piece and second heating piece still are equipped with the thick stick ear that the slope set up respectively, all be equipped with the removal hole that has predetermined height on the thick stick ear, wear to establish the round pin post in the removal hole, the middle part of round pin post still passes briquetting down.
According to the utility model discloses an automatic system of thawing of biological sample, thick stick ear has 10 ~ 30 degrees contained angles with vertical face.
According to the utility model discloses an automatic system of thawing of biological sample, remove the hole and follow the incline direction setting of thick stick ear.
According to the automatic biological sample unfreezing system, the elastic component comprises an elastic part and a bearing seat, an elastic hole is formed in the bearing seat, and the elastic part is arranged in the elastic hole; the bottom of the lower pressing block is provided with a thin end inserted into the elastic hole, and the elastic piece is sleeved with the thin end; the top of the adsorption piece penetrates into the elastic hole.
According to the utility model discloses an automatic system of unfreezing of biological sample, it is the electro-magnet to adsorb the piece, the default condition is the circular telegram.
According to the utility model discloses an automatic system of thawing of biological sample, be close to in the heating piece the position of cryopreserving pipe still is equipped with second temperature sensor.
According to the utility model discloses an automatic system of thawing of biological sample, be equipped with first heat insulating part down between briquetting and the cryopreserving pipe.
According to the utility model discloses an automatic system of unfreezing of biological sample, still cup joint the second heat insulating part on the round pin post, the second heat insulating part set up in the round pin post with the part of cup jointing of briquetting down.
According to the utility model discloses an automatic system of thawing of biological sample, the downthehole portion of heating piece is equipped with the heat-conducting layer.
The utility model aims at providing an automatic thawing system of biological sample, through setting up thawing structure and bullet tubular construction, thawing structure includes the heating block, be equipped with first temperature sensor and the second temperature sensor of real-time supervision heating block and cryopreserving pipe temperature respectively on the heating block, first temperature sensor and second temperature sensor transmit the temperature in real time to control system, time and the highest temperature of heating block heating are controlled through control system, make thawing system can adapt to different cell to the different requirements of temperature and time length; the elastic tube structure comprises a lower pressing block, the top of the lower pressing block is abutted against the bottom of the cryopreservation tube, the lower part of the lower pressing block is connected with the elastic assembly and the adsorption piece, and after the cryopreservation tube is thawed, the cryopreservation tube is automatically and quickly popped out through the elastic tube structure, so that the cryopreservation tube is separated from a high-temperature environment immediately after being thawed, the time is not prolonged, and the damage to cells caused by the recrystallization of ice crystals is avoided, and the cells are prevented from being dead; the heating piece includes first heating piece and second heating piece, unfreezes structural still being equipped with and embraces the subassembly tightly, embraces the thick stick ear that the subassembly set up including the slope tightly, all is equipped with on the thick stick ear and removes the hole, wears to establish the round pin post in removing the hole, the middle part of round pin post still passes down the briquetting, it does not have the space to make to freeze through embracing the subassembly and deposit between pipe and the heating hole, the reduction air makes the heat transmit to freeze and deposits on the pipe fast, reviving the cell of ultra-low temperature in the short time, avoid the death of cell. To sum up, the beneficial effects of the utility model are that: the heating temperature and the time of depositing the pipe through two temperature sensor accurate control freezes to pop out the freezing of accomplishing of unfreezing fast through bullet tubular construction and deposit the pipe, deposit pipe and heating hole in close contact with through holding tightly the structure messenger freezing, rapid heating up shortens cell thawing time, reduces the cell death rate.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Referring to fig. 1, the utility model provides an automatic system of thawing of biological sample, including structure and the bullet tube structure of thawing.
Referring to fig. 1 and 5, the thawing structure includes a heating block 1, a heating hole 11 for inserting the cryopreservation tube 7 is formed in the middle of the heating block 1, and the heating hole 11 penetrates in the vertical direction of the heating block 1. A heating element 12 for heating the heating block 1 is also inserted in the heating block 1, and the heating element 12 is an electric heating rod; the heating block 1 is provided with a first temperature sensor 13 for monitoring the heating temperature of the heating block 1 in real time, and the heating element 12 and the first temperature sensor 13 are both connected with a control system.
In the using process, the heating element 12 is connected with a power supply to heat the heating block 1, then the freezing tube 7 is inserted into the heating hole 11, and the freezing tube 7 is heated after the heating block 1 is heated to unfreeze the freezing tube 7; in the heating process of the heating block 1, the first temperature sensor 13 can transmit the temperature of the heating block 1 to the control system in real time, and the control system controls the heating time of the heating element 12 for the heating block 1 and the highest temperature reached by the heating block 1, so that the thawing system can adapt to different requirements on temperature and duration when different cells are thawed.
Referring to fig. 2, in order to better and directly monitor the temperature of the cryopreservation tube 7, as a preferred scheme, a second temperature sensor 14 is further arranged in the heating block 1 at a position close to the cryopreservation tube 7, the second temperature sensor 14 is an infrared sensor, and the second temperature sensor 14 is connected with a control system. In the use process, the temperature of the cryopreservation tube 7 is monitored in real time through the second temperature sensor 14, when the temperature of the cryopreservation tube 7 reaches the highest temperature capable of being borne by cells, signals are transmitted to the control system, and the control system controls related parts to be disconnected or started, so that the cells can rapidly pass through the most easily damaged temperature.
In order to improve the heat conduction efficiency of the heating block 1 to the cryopreservation tube 7, as a preferable scheme, a heat conduction layer is arranged inside the heating hole 11 of the heating block 1, and the heat conduction layer is solid heat conduction silica gel, so that the cryopreservation tube 7 is rapidly heated and thawed.
Referring to fig. 1 and 2, the elastic tube structure comprises a lower pressing block 3, the top of the lower pressing block 3 is inserted into a heating hole 11, the top of the lower pressing block 3 is abutted against the bottom of the cryopreservation tube 7, the lower part of the lower pressing block 3 is connected with an elastic assembly, the bottom of the lower pressing block 3 is correspondingly provided with an adsorption piece 4, and an adsorption force exists between the adsorption piece 4 and the lower pressing block 3 under a preset condition; the utility model discloses in, adsorb 4 connection control systems of piece to adsorb 4 adsorption affinity through control system control.
In the working process, the lower pressing block 3 is in the initial state that the bottom does not contact the adsorption piece 4. The staff makes adsorption element 4 have the adsorption affinity through control system, then will freeze and deposit pipe 7 and insert in heating hole 11 to push down and freeze and deposit pipe 7, because the bottom butt briquetting 3 down of freezing the pipe 7, consequently, freeze and deposit pipe 7 and push down the in-process, lower briquetting 3 is also pushed down indirectly, makes briquetting 3 contact adsorption element 4 down, and is adsorbed by adsorption element 4. At this time, the lower pressing block 3 is positioned lower and compresses the elastic component, the depth of the cryopreservation tube 7 in the heating hole 11 is larger, and the cryopreservation tube 7 is heated and unfrozen through the heating block 1; after the freezing pipe 7 is defrosted, the control system receives a signal transmitted by the first temperature sensor 13 or the second temperature sensor 14, the adsorption force of the adsorption piece 4 disappears, the adsorption piece 4 releases the adsorption on the lower pressing block 3, the lower pressing block 3 bounces upwards under the action of the elastic component, and the freezing pipe 7 pops out of the heating hole 11.
The utility model discloses in, lower briquetting 3 is for having the carbide of higher hardness and wearability, adsorbs 4 for the electro-magnets, and when control system was the electro-magnet circular telegram, the electro-magnet can adsorb briquetting 3 down, and when control system was the electro-magnet outage, the electro-magnet disappeared with the adsorption affinity between the briquetting 3 down, and the above-mentioned predetermined condition is the circular telegram promptly.
Referring to fig. 2 and 4, as an embodiment, the elastic component includes an elastic member 31 and a socket 32, the socket 32 has an elastic hole 33 therein, and the elastic member 31 is disposed in the elastic hole 33; the bottom of the lower pressing block 3 is provided with a thin end inserted into the elastic hole 33, and the elastic piece 31 is sleeved at the thin end of the lower pressing block 3; the top of the absorbent member 4 penetrates into the elastic hole 33.
In the preparation process, the diameter of the elastic hole 33 can be larger than the maximum outer diameter of the lower pressing block 3, so that the whole lower pressing block 3 can be movably inserted into the elastic hole 33, namely, when the lower pressing block 3 is pressed downwards, the lower pressing block 3 can be integrally pressed into the elastic hole 33. The staff can also adopt another preparation scheme, the length of the thin end of the lower pressing block 3 is larger than the hole depth of the elastic hole 33 and at least larger than 5cm, so that when the lower pressing block 3 is pressed down, enough pressing height difference exists.
Referring to fig. 6, as another embodiment, the elastic component includes an elastic member 31, a circular ring-shaped receiving seat 32 and a limiting lug 34 disposed on the lower pressing block 3, the elastic member 31 is sleeved outside the lower pressing block 3, the top end of the elastic member 31 is fixedly connected to the limiting lug 34, the bottom end of the elastic member 31 is fixedly connected to the receiving seat 32, and the bottom of the receiving seat 32 is fixedly connected to the adsorbing member 4.
In both embodiments, the elastic member 31 is a spring.
By adopting the scheme, the utility model, the intelligent heating of the freezing pipe 7 is realized, and the temperature of the freezing pipe 7 is controlled with high precision; simultaneously through the effect of briquetting 3, elastic component and adsorption element 4 down, realize freezing the automatic of depositing pipe 7 after the completion of thawing and pop out fast, omit the manual time of opening box or box-type device, make freezing the time of depositing pipe 7 break away from high temperature environment at once after thawing, do not have the time extension, avoid the ice crystal recrystallization to cause the injury to the cell, lead to the death of cell.
Of course, the utility model discloses being equipped with the alarm presetting the position, alarm connection control system receives freezing before depositing pipe 7 the signal that will unfreeze the completion 10 ~ 20s when control system, and the control alarm sounds, freezes to deposit pipe 7 and pops out the back, and the staff can thoroughly take off it fast.
Referring to fig. 1 and 5, the utility model discloses a heating block 1 includes first heating block 15 and second heating block 16, and first heating block 15 and second heating block 16 symmetry set up, refer to fig. 5, and first heating block 15 all is equipped with semi-circular heating arc 111 with the middle part of second heating block 16, and when first heating block 15 and second heating block 16 merge, heating hole 11 is constituteed just to two semi-circular heating arcs 111 for the insertion of cryopreserving pipe 7. Referring to fig. 1 and 3, the thawing structure of the present invention is further provided with a holding assembly, the holding assembly includes a supporting pillar 2 transversely penetrating the lower portions of the first heating block 15 and the second heating block 16, the first heating block 15 and the second heating block 16 can rotate around the supporting pillar 2, the two ends of the supporting pillar 2 are fixedly connected to the supporting plate 5, and the bottom of the supporting plate 5 is fixedly connected to the bottom plate 51; the two sides of the bottoms of the first heating block 15 and the second heating block 16 are respectively provided with a lever ear 21 which is obliquely arranged, the lever ear 21 and a vertical surface have an included angle of 10-30 degrees, the lever ear 21 is provided with a moving hole 22 with a preset height, the moving hole 22 is arranged along the oblique direction of the lever ear 21, a pin 23 is arranged in the moving hole 22 in a penetrating manner, and the middle of the pin 23 penetrates through the lower pressing block 3.
In the using process, when a worker presses the freezing tube 7 downwards, the lower pressing block 3 is also pressed downwards, the lower pressing block 3 drives the pin 23 to move downwards in the downward moving process, the pin 23 drives the lever ear 21 to rotate towards the left lower side in the downward moving process (taking the first heating block 15 and the lever ear 21 which can be seen from the view on the figure 3 as an example), the first heating block 15 on the upper part of the lever ear 21 rotates towards the right upper side according to the lever principle, and according to the analysis, the first heating block 15 and the second heating block 16 move in opposite directions, so that the first heating block 15 and the second heating block 16 are combined, and the two semicircular heating arcs 111 tightly hold the freezing tube 7 and are in tight contact with the freezing tube 7; when the freezing tube 7 is thawed, and the lower pressing block 3 springs upward, the pin 23 is driven to move upward, the pin 23 moves upward, and in the process that the pin 23 moves upward (taking the first heating block 15 and the lever ear 21 as an example which can be seen from the view on fig. 3), the lever ear 21 is driven to rotate rightward and upward, and according to the lever principle, the first heating block 15 on the upper part of the lever ear 21 rotates leftward and downward, so that the first heating block 15 and the second heating block 16 move backward, the first heating block 15 and the second heating block 16 are separated, the two semicircular heating arcs 111 release the clasping of the freezing tube 7, and the freezing tube 7 can be smoothly sprung upward.
Through above-mentioned technical scheme, it heats in inserting the thermohole 11 to freeze to deposit pipe 7, first heating block 15 and second heating block 16 can hold tightly and freeze and deposit pipe 7, make and freeze and do not have the space between depositing pipe 7 and the thermohole 11, the existence of air has been reduced in a large number, make heat in first heating block 15 and the second heating block 16 transmit to freezing on depositing pipe 7 fast, make and freeze and deposit pipe 7 rapid heating, rapid heating up and unfreeze, revive the cell of ultra-low temperature in the short time, avoid the ice crystal recrystallization to cause the injury to the cell, lead to the death of cell.
Referring to fig. 2, as a preferred scheme, a first heat insulation member 6 is arranged between the lower pressing block 3 and the cryopreservation tube 7, and the first heat insulation member 6 can prevent heat on the cryopreservation tube 7 from being transferred to the lower pressing block 3, so that the cryopreservation tube 7 is prevented from transferring heat to the adsorption member 4 (electromagnet), and the adsorption member 4 is prevented from being out of work; the pin 23 is also sleeved with a second heat insulation piece 61, the second heat insulation piece 61 is arranged on the sleeved part of the pin 23 and the lower press block 3, namely the second heat insulation piece 61 is arranged between the pin 23 and the lower press block 3; because first heating block 15 and second heating block 16 all are connected with lever ear 21, heat on first heating block 15 and the second heating block 16 can transmit on lever ear 21, also indirectly transmit on round pin 23, second heat insulating part 61 can prevent to transmit on the round pin 23 pushes down the briquetting 3 on, avoids freezing and depositing pipe 7 to pass on the heat adsorbs piece 4 (electro-magnet), prevents to adsorb 4 inefficacy.
The utility model discloses in, the hard alloy of round pin post 23 for having higher hardness and wearability, the material of heating piece 1 is aluminium.
Because the freezing pipe among the prior art generally is three kinds of specifications, 1.5ml, 1.8 ~ 2.0ml and 6ml, consequently, to current three kinds of freezing pipes, the heating hole 11 in the system of unfreezing can set up three kinds of specifications, and in a system of unfreezing, can set up the heating hole 11 of three kinds of specifications simultaneously, of course, also can set up the heating hole 11 of other specifications according to market needs. Utility model people reachs through a plurality of experiments, the utility model discloses a system of unfreezing can melt the freezing pipe of depositing more than-80 ℃ within 1 minute, after the completion of unfreezing, the final temperature of freezing the pipe of depositing is not higher than 5 ℃, avoids the cell activity impaired.
To sum up, the utility model discloses a set up thawing structure and bullet pipe structure, thawing structure includes the heating block, is equipped with first temperature sensor and second temperature sensor who monitors the heating block and cryopreserved pipe temperature respectively in real time on the heating block, and first temperature sensor and second temperature sensor transmit the temperature to control system in real time, through control system control heating block heating time and the highest temperature, make thawing system can adapt to different cell to the different requirements of temperature and time length; the elastic tube structure comprises a lower pressing block, the top of the lower pressing block is abutted against the bottom of the cryopreservation tube, the lower part of the lower pressing block is connected with the elastic assembly and the adsorption piece, and after the cryopreservation tube is thawed, the cryopreservation tube is automatically and quickly popped out through the elastic tube structure, so that the cryopreservation tube is separated from a high-temperature environment immediately after being thawed, the time is not prolonged, and the damage to cells caused by the recrystallization of ice crystals is avoided, and the cells are prevented from being dead; the heating piece includes first heating piece and second heating piece, unfreezes structural still being equipped with and embraces the subassembly tightly, embraces the thick stick ear that the subassembly set up including the slope tightly, all is equipped with on the thick stick ear and removes the hole, wears to establish the round pin post in removing the hole, the middle part of round pin post still passes down the briquetting, it does not have the space to make to freeze through embracing the subassembly and deposit between pipe and the heating hole, the reduction air makes the heat transmit to freeze and deposits on the pipe fast, reviving the cell of ultra-low temperature in the short time, avoid the death of cell. To sum up, the beneficial effects of the utility model are that: the heating temperature and the time of depositing the pipe through two temperature sensor accurate control freezes to pop out the freezing of accomplishing of unfreezing fast through bullet tubular construction and deposit the pipe, deposit pipe and heating hole in close contact with through holding tightly the structure messenger freezing, rapid heating up shortens cell thawing time, reduces the cell death rate.
Naturally, the present invention can be embodied in many other forms without departing from the spirit or essential attributes thereof, and it should be understood that various changes and modifications can be made by one skilled in the art without departing from the spirit or essential attributes thereof, and it is intended that all such changes and modifications be considered as within the scope of the appended claims.