CN213933714U - High-sensitivity liquid chromatography detection system - Google Patents
High-sensitivity liquid chromatography detection system Download PDFInfo
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- CN213933714U CN213933714U CN202022462519.5U CN202022462519U CN213933714U CN 213933714 U CN213933714 U CN 213933714U CN 202022462519 U CN202022462519 U CN 202022462519U CN 213933714 U CN213933714 U CN 213933714U
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Abstract
A high-sensitivity liquid chromatography detection system comprises a liquid path joint group, a coupling lens group and a liquid path joint, wherein the liquid path joint group comprises an upper liquid path joint (1) and a lower liquid path joint (2), and the coupling lens group comprises an upper coupling lens (6) and a lower coupling lens (7); liquid flows in from the upper liquid path joint (1) and flows out from the lower liquid path joint (2); the front side of the upper liquid path joint (1), the rear side and the middle part of the lower liquid path joint (2) are provided with a liquid path and a light path guide pipe (3); the top of the upper liquid path joint (1) is provided with a light path coupling joint (4), the top of the light path coupling joint (4) is provided with a light source (5), the joint of the light path coupling joint (4) and the upper liquid path joint (1) is provided with an upper coupling lens (6), and the bottom of the lower liquid path joint (2) is provided with a lower coupling lens (7). The utility model discloses can increase the optical path, improve and detect the limit, the multiple of improvement can reach tens times or higher.
Description
Technical Field
The utility model relates to a biochemical analysis, more specifically say and be used for the high sensitivity liquid chromatography detecting system of analysis material chemical composition and preparation separation purification.
Background
The liquid chromatographic detection system is an instrument which firstly separates a mixture and then analyzes and identifies the mixture by utilizing the difference of the distribution ratio of the mixture between liquid-solid or immiscible two liquids. The liquid chromatograph is further classified into liquid-liquid chromatography LLC and liquid-fixation chromatography LSC according to whether the stationary phase is liquid or solid. The modern liquid chromatograph consists of a high-pressure infusion pump, a sample introduction system, a temperature control system, a chromatographic column, a detector, a signal recording system and the like. Compared with the classical liquid phase column chromatography device, the device has the characteristics of high efficiency, rapidness, sensitivity and the like.
The system consists of a liquid storage device, a pump, a sample injector, a chromatographic column, a detector, a recorder and the like. The mobile phase in the liquid storage device is pumped into the system by a high pressure pump, the sample solution enters the mobile phase through the sample injector and is loaded into the stationary phase of the chromatographic column by the mobile phase, and because each component in the sample solution has different distribution coefficients in two phases, when the two phases move relatively, through the distribution process of repeated adsorption-desorption, each component generates a large difference in moving speed and is separated into single components which flow out of the column in sequence, when the sample passes through the detector, the concentration of the sample is converted into an electric signal and is transmitted to the recorder, and data is printed out in a map form to form the high performance liquid chromatograph which mainly comprises a sample injection system, a liquid infusion system, a separation system, a detection system and a data processing system. A detector: and converting the sample components continuously flowing out of the chromatographic column into electric signals which are easy to measure, and receiving the electric signals by a data system to obtain a chromatogram map of sample separation. The detector consists of a light source, a monochromator light path, a sample cell, a detector, a signal acquisition circuit and a control and communication circuit.
The working principle of the system is that ultraviolet monochromatic light is converged by a lens and passes through a flow cell to reach a photoelectric detector, when no absorption sample passes through the flow cell, a photoelectric signal is a signal passing through a mobile phase or an empty cell, and the signal is in a baseline state. As the liquid flows through the flow cell. If there is a separate absorption sample in the liquid, an absorption peak occurs, which is plotted in fig. 3. The abscissa is a time axis, and the ordinate is a signal value of an absorption peak, and the content can be quantitatively calculated according to the area.
Definition of optical path, beer's law: a = log (I0/I) = epsilon CL; a is the absorption rate; i0 represents the light intensity of the reference cell; i is the light intensity of the sample cell; epsilon is a molar absorptivity coefficient; c is the sample concentration; l is the optical path of the flow cell.
The optical paths are usually very short, 0.1mm or 1-2mm, and not more than 10mm at the maximum, which has the disadvantage that the liquid paths are usually relatively narrow or short in diameter, the optical path is limited, and the detection limit is therefore limited.
For liquid phase detection of a trace amount of sample, a light path is designed in a manner that a liquid flow path is a quartz capillary, when light passes through the capillary, the diameter of the capillary corresponds to the light path of the detection light path, and the diameter of the capillary is usually only a few hundred micrometers, so that the light path is very short, and in the case of the light path, the detection limit and the sensitivity are determined to be much lower than those of liquid chromatography.
In order to solve the problem, the utility model provides a high sensitivity liquid chromatogram detecting system.
Disclosure of Invention
In order to solve the problem that exists among the above-mentioned prior art, the utility model provides a high sensitivity liquid chromatography detecting system can increase the optical path, improves and detects the limit, and the multiple of improvement can reach tens times or higher.
The utility model provides a technical scheme that its technical problem adopted is:
a high-sensitivity liquid chromatography detection system comprises a conduit 3 shared by a light path and a liquid path, a light path coupling joint 4, a liquid path joint group and a coupling lens group;
the liquid path joint group comprises an upper liquid path joint 1 and a lower liquid path joint 2, and the coupling lens group comprises an upper coupling lens 6 and a lower coupling lens 7;
liquid flows in from the upper liquid path joint 1 and flows out from the lower liquid path joint 2; the front side of the upper liquid path joint 1, the rear side and the middle part of the lower liquid path joint 2 are provided with a light path and a liquid path conduit 3;
the inner surface of the part shared by the light path and the liquid path is specially treated to ensure that the surface of the part has high reflectivity, when light enters the liquid path/the light path, the high reflectivity can ensure that the light has multiple reflectivity on the inner wall of the liquid path, and the light reflected each time permeates the liquid again, so that a long optical path is formed after passing through the liquid for multiple times, the absorption of signals is enhanced, and the light is emitted to the lower coupling mirror after being absorbed.
The top of the upper liquid path joint 1 is provided with a light path coupling joint 4, the top of the light path coupling joint 4 is provided with a light source 5, the joint of the light path coupling joint 4 and the upper liquid path joint 1 is provided with an upper coupling lens 6, and the bottom of the lower liquid path joint 2 is provided with a lower coupling lens 7.
The utility model discloses still have following additional technical characterstic:
as the utility model discloses technical scheme further specifically optimizes: the bottom of the lower coupling lens 7 is provided with a detector 8.
As the utility model discloses technical scheme further specifically optimizes: the bottom of the lower coupling lens 7 is provided with an ultraviolet fiber spectrometer 9.
Compared with the prior art, the utility model, its advantage lies in:
the method has the advantages that: a longitudinal light guide pipeline is adopted as a common passage for light and liquid; the length of the light path can be changed automatically according to the flow and the content of the liquid; the thickness of the light path is automatically changed according to the flow and the content of the liquid; the optical path is longer and the detection limit is lower.
The method has the advantages that: the optical path length can be extended to the required optical path length; the detection limit is low, and a sample with low content can be detected; does not affect the working characteristics of other separation devices, such as separation columns or high pressure; the length of the light path can be changed automatically according to the flow and the content of the liquid; the thickness of the light path is automatically changed according to the flow and the content of the liquid; the detection range is wider, the detection is not influenced by the concentration of the sample volume or the amount of the sample, and the compatibility is higher.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic diagram of the system structure of the present invention;
fig. 2 is a schematic diagram of the system structure of the present invention;
fig. 3 is a three-dimensional spectrum and a spectrogram of an absorption spectrum of the present invention;
description of reference numerals: an upper liquid path joint 1; a lower liquid path joint 2; a light path and liquid path conduit 3; an optical path coupling joint 4; a light source 5; an upper coupling lens 6; a lower coupling lens 7; a detector 8; uv fiber optic spectrometer 9.
Detailed Description
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings, in order that the present disclosure may be more completely understood, and the scope of the present disclosure may be fully conveyed to those skilled in the art. While the exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure is not limited to the embodiments set forth herein.
A high-sensitivity liquid chromatography detection system changes the transverse direction of a detection light path into the longitudinal direction, defines the detection light path as a detection light path unit capable of changing parameters such as length, thickness and the like, expands the light path of the light from um and mm level to dozens of mm or even hundreds of mm level through the liquid path, can be changed according to the requirement of liquid concentration, changes the input of the light from fixed input into fixed input or optical fiber input and other modes, is more flexible to operate, and can increase the detection sensitivity by one step.
The high-sensitivity liquid chromatography detection system comprises a liquid path conduit 3, a light path coupling joint 4, a liquid path joint group and a coupling lens group.
The liquid path joint group comprises an upper liquid path joint 1 and a lower liquid path joint 2, and the coupling lens group comprises an upper coupling lens 6 and a lower coupling lens 7.
Liquid flows in from the upper liquid path joint 1 and flows out from the lower liquid path joint 2; the front side of the upper fluid passage joint 1 and the rear side and the middle part of the lower fluid passage joint 2 are provided with fluid passage guide pipes 3.
The top of the upper liquid path joint 1 is provided with a light path coupling joint 4, the top of the light path coupling joint 4 is provided with a light source 5, the joint of the light path coupling joint 4 and the upper liquid path joint 1 is provided with an upper coupling lens 6, and the bottom of the lower liquid path joint 2 is provided with a lower coupling lens 7.
The bottom of the lower coupling lens 7 is provided with a detector 8, and the system structure is shown in FIG. 1.
The bottom of the lower coupling lens 7 is provided with an ultraviolet fiber spectrometer 9, and the system structure is shown in fig. 2.
The working process of the high-sensitivity liquid chromatography detection system comprises the following steps:
the liquid normally flows through the liquid path, the light path irradiates into the liquid path through the upper coupling lens 6, and the light path irradiates onto the detector 8 from the lower coupling lens 7 through the absorption of a long light path in the liquid path to obtain a light absorption signal of the sample light path; the reference optical path is reserved in the other path of the same optical path. Software is required to enable the parameters to be modified when a different detection unit is replaced.
The testing unit adopts the light path liquid path guide pipe 3 as a common path of light and liquid, keeps the same path on a long path, is provided with the upper coupling lens 6 and the lower coupling lens 7 near an inlet and an outlet, can be matched with a light splitting light path of a liquid phase detector for testing, can also adopt an optical fiber leading-in mode to externally connect an input optical signal, and finally realizes a large light absorption light path.
The large-capacity sample can be detected by selecting a thick pipeline, and the trace sample can be detected by adopting a miniature light guide pipeline without influencing the flow characteristics of the separated liquid. In addition, the length of the detection pool can be selected according to the width of the separation peak.
The system is extended as follows. When the system adopts composite ultraviolet light input, the structure diagram of the system is shown as figure 2, and a spectrum direct receiving mode is adopted to obtain a three-dimensional spectrum and a spectrogram 3 of an absorption spectrum. After passing through a liquid phase detection system, a three-dimensional spectrum can be obtained, the longitudinal direction is an absorption peak, one dimension is a time axis, and one dimension is a spectrum, so that qualitative and quantitative analysis can be performed.
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the above description, in combination with the drawings in the embodiments of the present invention, clearly and completely describes the technical solutions in the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the above detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Claims (3)
1. A high-sensitivity liquid chromatography detection system is characterized by comprising a liquid path, a light path guide pipe (3), a light path coupling joint (4), a liquid path joint group and a coupling lens group;
the liquid path joint group comprises an upper liquid path joint (1) and a lower liquid path joint (2), and the coupling lens group comprises an upper coupling lens (6) and a lower coupling lens (7);
the inner surfaces of the liquid path conduit and the light path conduit (3) are specially processed into high reflectivity;
liquid flows in from the upper liquid path joint (1) and flows out from the lower liquid path joint (2); the front side of the upper liquid path joint (1), the rear side and the middle part of the lower liquid path joint (2) are provided with a liquid path and a light path guide pipe (3);
the top of the upper liquid path joint (1) is provided with a light path coupling joint (4), the top of the light path coupling joint (4) is provided with a light source (5), the joint of the light path coupling joint (4) and the upper liquid path joint (1) is provided with an upper coupling lens (6), and the bottom of the lower liquid path joint (2) is provided with a lower coupling lens (7).
2. The high sensitivity liquid chromatography detection system of claim 1, wherein: the bottom of the lower coupling lens (7) is provided with a detector (8).
3. The high sensitivity liquid chromatography detection system of claim 1, wherein: the bottom of the lower coupling lens (7) is provided with a photoelectric detector or an ultraviolet fiber spectrometer (9).
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| CN202022462519.5U CN213933714U (en) | 2020-10-30 | 2020-10-30 | High-sensitivity liquid chromatography detection system |
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| CN202022462519.5U CN213933714U (en) | 2020-10-30 | 2020-10-30 | High-sensitivity liquid chromatography detection system |
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