CN213854916U

CN213854916U - Integration adipose tissue stem cell extraction element

Info

Publication number: CN213854916U
Application number: CN202022337029.2U
Authority: CN
Inventors: 杨琨; 陈伟基; 丁海娥; 李铀; 冯哲; 卢利平; 魏锁成
Original assignee: Northwest Minzu University
Current assignee: Northwest Minzu University
Priority date: 2020-10-19
Filing date: 2020-10-19
Publication date: 2021-08-03
Anticipated expiration: 2030-10-19

Abstract

The utility model relates to a cell extraction element, concretely relates to integration adipose tissue stem cell extraction element. The problems of multiple types of equipment, easy pollution of transfer and low dissolving efficiency in the conventional stem cell extraction are effectively solved; the device comprises a crushing unit, a grinding unit and a dissolving and filtering unit, wherein the crushing unit, the grinding unit and the dissolving and filtering unit are sequentially and vertically arranged; the motor is arranged on the crushing unit through the mounting frame and drives the crushing unit, the grinding unit and the dissolving and filtering unit to work through the rotating shaft; and the liquid discharge barrel is communicated with the dissolving and filtering unit. This device is through adopting the broken mode of smashing the unit and mutually supporting of grinding unit, and the broken adipose tissue of maximize adopts the filtration unit that dissolves of reciprocal stirring formula simultaneously, can be quick dissolve through collagenase, improves dissolving efficiency.

Description

Integration adipose tissue stem cell extraction element

Technical Field

The utility model relates to a cell extraction element, concretely relates to integration adipose tissue stem cell extraction element.

Background

Stem cells are primitive cells having self-renewal and multipotential differentiation potential, which are cells of origin of the body and can differentiate into one or more cells constituting human tissues or organs under specific conditions. Self-renewal refers to the ability of stem cells to maintain the size of stem cell populations through cell division; the multipotential differentiation means that the stem cells have plasticity and can be differentiated into different tissue cells (for example, mesenchymal stem cells can be differentiated into nerve cells, osteoblasts, cardiac muscle cells and the like) under different in vivo environments so as to form various complex tissue organs of the body. Mesenchymal Stem Cells (MSCs) are known as Mesenchymal Stem cells because they can differentiate into Mesenchymal tissues, have sub-totipotent differentiation potential, and can be induced to differentiate into various tissue cells in specific in vivo and in vitro environments. Mesenchymal stem cells have the commonality of stem cells, i.e., the biological characteristics of self-renewal and multipotentiality; in addition, the mesenchymal stem cells also have the characteristic of in vitro amplification.

At present, adipose-derived stem cells are used by extracting adipose-derived stem cells from adipose tissues after collagenase digestion, separating the adipose-derived stem cells to obtain pure adipose-derived stem cells, and then mixing the adipose-derived stem cells with the adipose tissues for filling so as to improve the survival rate of the adipose-derived stem cells.

The existing stem cell extraction equipment adopts different equipment for fat tissue crushing and collagenase solution digestion, the equipment cost is high, the transfer of fat tissue is required in the operation process, and the pollution is easy. Meanwhile, the crushed adipose tissues still have larger particles, the collagenase solution has long dissolution and digestion time and low efficiency.

SUMMERY OF THE UTILITY MODEL

The utility model provides an integration adipose tissue stem cell extraction element, the effectual equipment type that adopts when having solved current stem cell and drawing is many, shifts the problem of easily polluting and dissolving inefficiency, and this device is through adopting crushing unit and the broken mode of grinding unit mutually supporting, and the broken adipose tissue of maximize adopts the filtration unit that dissolves of reciprocal stirring formula simultaneously, can be quick dissolve through collagenase dissolution, improves dissolving efficiency.

In order to achieve the above object, the utility model adopts the following technical scheme:

an integration adipose tissue stem cell extraction element which characterized in that: the device comprises a crushing unit 1, a grinding unit 2 and a dissolving and filtering unit 3, wherein the crushing unit 1, the grinding unit 2 and the dissolving and filtering unit 3 are sequentially and vertically arranged;

the device also comprises a motor 6 which is arranged on the crushing unit 1 through a mounting rack 7, and the motor 6 drives the crushing unit 1, the grinding unit 2 and the dissolving and filtering unit 3 to work through a rotating shaft 8;

and a liquid discharge barrel 4 communicated with the dissolving and filtering unit 3.

The crushing unit 1 is of an inverted cone structure and consists of an outer crushing frame 9 and an inner crushing frame 10, and the rotating shaft 8 drives the inner crushing frame 10 to rotate; the inner surface of the crushing outer frame 9 is provided with spiral convex teeth 901; the outer surface of the crushing inner frame 10 is provided with a cutter 1001 of a spiral structure.

The grinding unit 2 consists of a grinding outer frame 11 and a grinding inner frame 12, and the rotating shaft 8 drives the grinding inner frame 12 to rotate; an outer grinding table 1101 is arranged on the inner surface of the grinding outer frame; an inner grinding table 1201 is arranged on the outer surface of the grinding inner frame 12; the outer grinding table 1101 and the inner grinding table 1201 are helical structures.

The dissolving and filtering unit 3 takes a dissolving and filtering barrel 13 as a carrier, a reciprocating screw rod 15 inside the dissolving and filtering barrel 13 is driven by a rotating shaft 8, and a sieve tray 16 is arranged on the reciprocating screw rod 15.

The dissolving and filtering barrel 13 is a double-layer barrel, and a heating pipe 14 is arranged between the double layers.

Dissolve that filter vat 13 is inside to be provided with draw-in groove 1301 and discharge gate 1302, be provided with card platform 1601 on sieve tray 16, card platform 1601 nests in draw-in groove 1301, and the restriction is filtered 16's rotation.

A bracket 19 is arranged between the crushing unit 1 and the dissolving and filtering unit 3, a collagenase solution barrel 5 is arranged on the bracket 19, and a discharge pipe of the collagenase solution barrel 5 is arranged in the dissolving and filtering barrel 13.

The liquid discharge barrel 4 is connected to the bottom of the dissolving and filtering barrel 13 through a connecting pipe 17, and a liquid discharge pipe 18 is arranged on the material discharge barrel 4.

The utility model has the advantages that: 1) this device will smash unit and grind the unit collection in an organic whole, broken adipose tissue that can the maximize reduces the follow-up degree of difficulty that dissolves.

2) The dissolving and filtering unit of the device adopts a reciprocating type dissolving and filtering structure, the filtering, stirring and dissolving are integrated, and the filtering structure can effectively prevent the broken adipose tissues from flowing into the liquid discharge barrel. The stirring can accelerate the dissolution of adipose tissues and collagenase, the dissolution efficiency is improved, and meanwhile, the heating unit is arranged, so that the dissolution efficiency can be further improved.

3) This device will smash unit, grinding unit and dissolve the filtration unit collection in an organic whole, compares the transfer between each equipment of transmission, can effectually prevent the pollution of transfer in-process, reduces equipment cost simultaneously.

4) The device is convenient to clean, adopts the communication design of all processes, and can realize synchronous cleaning of three units by one-time water injection.

In conclusion, this device is through adopting the broken mode of smashing the unit and mutually supporting of grinding unit, the broken adipose tissue of maximize, adopts the dissolving filter unit of reciprocal stirring formula simultaneously, can be quick dissolve through collagenase, improves dissolving efficiency.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a sectional view of the present invention;

FIG. 3 is a schematic view of a crushing unit;

FIG. 4 is a schematic view of a polishing unit;

FIG. 5 is a schematic view of a dissolving filter unit;

shown in the figure: the device comprises a crushing unit 1, a grinding unit 2, a dissolving and filtering unit 3, a liquid discharge barrel 4, a collagenase solution barrel 5, a motor 6, a mounting rack 7, a rotating shaft 8, a crushing outer rack 9, a spiral convex tooth 901, a crushing inner rack 10, a cutter 1001, a grinding outer rack 11, an outer grinding table 1101, a grinding inner rack 12, an inner grinding table 1201, a dissolving and filtering barrel 13, a clamping groove 1301, a discharge hole 1302, an electric heating pipe 14, a reciprocating screw rod 15, a screen disc 16, a clamping table 1601, a connecting pipe 17, a liquid discharge pipe 18 and a support 19.

Detailed Description

For making the utility model discloses a utility model purpose, technical scheme, beneficial effect are clearer, combine the figure in the following to explain in detail the embodiment of the utility model.

Example 1

As shown in figures 1 and 2, the utility model provides an integration adipose tissue stem cell extraction element aims at providing one kind and collects crushing, grinds and stirring and dissolves the filtration in the equipment of an organic whole, and this whole flow of equipment is smashed, is ground, is stirred and dissolves, filters and flowing back in proper order, and corresponding constitutional unit is crushing unit 1, grinding unit 2, dissolves filter unit 3 and flowing back bucket 4, and concrete structure is as follows.

As shown in fig. 3, the pulverizing unit 1 is composed of an outer pulverizing frame 9 and an inner pulverizing frame 10, and is integrally formed in an inverted cone shape, so that the cut adipose tissues can fall conveniently, and the cutting fineness can be ensured. Be provided with spiral convex tooth 901 on smashing the outer frame 9, be provided with the cutter 1001 of spiral on smashing inner frame 10, put into on smashing unit 1 adipose tissue after, through the cutting of cutter 1001 and the cooperation of spiral convex tooth 901, quick cutting adipose tissue, helical structure can transport the adipose tissue after the cutting to the bottom simultaneously.

As shown in fig. 4, the grinding unit 2 is installed at the bottom of the crushing unit 1, and is composed of a grinding outer frame 11 and a grinding inner frame 12, and the specific installation is as follows: the grinding outer frame 11 is fixed to the crushing outer frame 9, and the grinding outer frame 12 is fixed to the crushing inner frame 10 so as to rotate in synchronization with the crushing inner frame 10. An outer grinding table 1101 is arranged on the grinding outer frame 11, an inner grinding table 1201 is arranged on the grinding inner frame 12, the outer grinding table 1101 and the inner grinding table 1201 both adopt bosses with spiral structures, high-friction cuspids are arranged on the surfaces of the bosses, and the adipose tissues are ground through the rotation of the inner grinding table 1201.

As shown in fig. 5, the dissolving and filtering unit 5 uses the dissolving and filtering barrel 13 as a carrier, and is specifically installed such that the upper end surface of the dissolving and filtering barrel 13 is fixedly installed with the grinding outer frame 11. The upper end surface of the volume filter barrel 13 is provided with a discharge hole 1302, and the discharge hole 1302 is used for manually cleaning the undissolved adipose tissues. Meanwhile, a bracket 19 is arranged between the crushing outer frame 9 and the dissolving and filtering barrel 13, a collagenase dissolving barrel 5 is arranged on the bracket 19, and a collagenase solvent in the collagenase dissolving barrel 5 is injected into the dissolving and filtering barrel 13 from a discharge hole 1302 through a liquid outlet pipe.

The dissolving and filtering barrel 13 is a double-layer barrel, an electric heating tube 14 is arranged between the double layers, and the fastest dissolving temperature, namely 37 ℃, is kept in the dissolving barrel 13 by heating of the electric heating tube 14, so that the dissolving is accelerated.

Dissolving in the filter vat 13 and using reciprocal lead screw 15 as the actuating lever, installing sieve tray 16 on reciprocal lead screw 15, for guaranteeing that sieve tray 16 only produces reciprocating motion from top to bottom, be provided with draw-in groove 1301 on dissolving filter vat 13, set up ka tai 1601 on sieve tray 16 for ka tai 1601 pegs graft in draw-in groove 1301, restriction sieve tray 16's rotation.

The rotation of the crushing inner frame 10 of the crushing unit 1, the rotation of the inner grinding table 1201 of the grinding unit, and the rotation of the reciprocating screw 15 are all driven by a motor 6, the motor 6 is mounted on a mounting frame 7 on the crushing unit 1, the motor 6 drives a rotating shaft 8 to rotate, and the rotation of the crushing inner frame 10, the rotation of the inner grinding table 1201 of the grinding unit, and the rotation of the reciprocating screw 15 are driven by the rotating shaft 8.

The solution dissolved by the dissolving and filtering unit 3 is discharged through the liquid discharge barrel 4, the liquid discharge barrel 4 is connected to the bottom of the dissolving and filtering barrel 13 through a connecting pipe 17, a liquid discharge pipe 18 is arranged on the material discharge barrel 4, and the solution flowing into the liquid discharge barrel 4 is discharged through the liquid discharge pipe 18 to perform a collection test.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. An integration adipose tissue stem cell extraction element which characterized in that: the device comprises a crushing unit, a grinding unit and a dissolving and filtering unit, wherein the crushing unit, the grinding unit and the dissolving and filtering unit are sequentially and vertically arranged;

the motor is arranged on the crushing unit through the mounting frame and drives the crushing unit, the grinding unit and the dissolving and filtering unit to work through the rotating shaft;

and the liquid discharge barrel is communicated with the dissolving and filtering unit.

2. The integrated adipose tissue stem cell extraction device of claim 1, wherein: the crushing unit is of an inverted cone structure and consists of an outer crushing frame and an inner crushing frame, and the rotating shaft drives the inner crushing frame to rotate; the inner surface of the crushing outer frame is provided with spiral convex teeth; the outer surface of the crushing inner frame is provided with a cutter with a spiral structure.

3. The integrated adipose tissue stem cell extraction device of claim 1, wherein: the grinding unit consists of a grinding outer frame and a grinding inner frame, and the rotating shaft drives the grinding inner frame to rotate; an outer grinding table is arranged on the inner surface of the grinding outer frame; an inner grinding table is arranged on the outer surface of the grinding inner frame; the outer grinding table and the inner grinding table are of spiral structures.

4. The integrated adipose tissue stem cell extraction device of claim 1, wherein: the dissolving and filtering unit takes a dissolving and filtering barrel as a carrier, a reciprocating lead screw in the dissolving and filtering barrel is driven by a rotating shaft, and a sieve tray is arranged on the reciprocating lead screw.

5. The integrated adipose tissue stem cell extraction device of claim 4, wherein: the dissolving and filtering barrel is a double-layer barrel, and a heating pipe is arranged between the double layers.

6. The integrated adipose tissue stem cell extraction device of claim 5, wherein: dissolve inside draw-in groove and the discharge gate of being provided with of filter vat, be provided with the card platform on the sieve tray, card platform nestification is in the draw-in groove, the rotation of restriction screening.

7. The integrated adipose tissue stem cell extraction device of claim 1, wherein: a bracket is arranged between the crushing unit and the dissolving and filtering unit, a collagenase solution barrel is arranged on the bracket, and a discharge pipe of the collagenase solution barrel is arranged in the dissolving and filtering barrel.

8. The integrated adipose tissue stem cell extraction device of claim 1, wherein: the liquid discharge barrel is connected to the bottom of the dissolving and filtering barrel through a connecting pipe, and a liquid discharge pipe is arranged on the material discharge barrel.