CN213842814U - Simple and easy purified protein pipeline - Google Patents
Simple and easy purified protein pipeline Download PDFInfo
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- CN213842814U CN213842814U CN202021735041.2U CN202021735041U CN213842814U CN 213842814 U CN213842814 U CN 213842814U CN 202021735041 U CN202021735041 U CN 202021735041U CN 213842814 U CN213842814 U CN 213842814U
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Abstract
The utility model discloses a simple and easy purification protein pipeline, including sample loading unit, conventional pipe, pumping pressure pipe, peristaltic pump, ventilative unit, chromatography post. The sample loading unit comprises a three-way valve, and an injector and a reagent bottle which are connected with the three-way valve. The gas permeable unit comprises an inlet end, an outlet end provided with a valve and a gas permeable end. The sample loading unit sucks reagent, and the reagent solution passes through the pump pressure conduit under the action of the peristaltic pump, and air is discharged through the air permeable unit and enters the chromatographic column. The simple protein purification pipeline has the advantages of simple structure, low cost, easy operation, time saving and labor saving.
Description
Technical Field
The utility model belongs to the technical field of relate to biochemical research apparatus, in particular to simple and easy protein purification pipeline.
Background
Automated protein purification devices are currently known, such as those manufactured by the U.S. general-purpose company
The purification system, the protein purification system produced by bole bio-rad company, the Clear First protein purification system produced by shanghai flash spectrum biotechnology company, and the like are expensive, and the operator needs to be trained, so that the use threshold is high, and the wide popularization is difficult.
In addition to the use of automated equipment, most pre-cartridges commonly available on the market also support the use of syringes or peristaltic pumps, such as the hisscreen and HiTrap series manufactured by the american general company, chromatography pre-cartridges manufactured by shanghai, etc. The syringe needs to be pressed for a long time, the time and the labor are wasted, and the peristaltic pump lacks the support of a matched pipeline instrument.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a: the problem of need manpower to press for a long time, waste time and energy in the existing market, use peristaltic pump then lack supporting pipeline apparatus is solved, consequently provide one kind and go up appearance volume big, low cost, simple structure, the protein purification pipeline of easy operation.
The utility model adopts the technical scheme as follows:
a simple protein purification pipeline comprises a sample loading unit, a conventional conduit, a pumping pressure conduit, a peristaltic pump, an air permeable unit and a chromatographic column, wherein one end of the conventional conduit is connected with the sample loading unit, the other end of the conventional conduit is connected with the peristaltic pump, the pumping pressure conduit penetrates through the peristaltic pump to be connected with the inlet end of the air permeable unit, and the outlet end of the air permeable unit is connected with the chromatographic column.
Preferably, the sample loading unit comprises a three-way valve, a reagent bottle and an injector, wherein the first end of the three-way valve is connected with the pumping pressure conduit, the second end of the three-way valve is connected with the injector, the third end of the three-way valve is connected with the reagent bottle through a conventional conduit, and the pumping pressure conduit can be only communicated with the injector or the pumping pressure conduit is only communicated with the reagent bottle under the action of the three-way valve. The operator can select to use a syringe to load the sample or a reagent bottle to load the sample according to the specification, the sample loading volume or the reagent capacity of the chromatographic column.
Preferably, the ventilation unit is made of transparent material and is horizontal and vertical, the top of the ventilation unit is provided with an inlet end and a ventilation end, and the bottom of the ventilation unit is provided with an outlet end.
Preferably, the length of the pumping pressure conduit is the length required for realizing the function of the peristaltic pump, the connectors are arranged at two ends of the pumping pressure conduit, and an operator can realize the control of the required pumping pressure by adjusting the rotating speed of the peristaltic pump and replacing the pumping pressure conduits with different calibers.
Preferably, the ventilating unit is made of transparent materials and is vertical to the horizontal direction, the top of the ventilating unit is provided with an inlet end and a ventilating end, and the bottom of the ventilating unit is provided with an outlet end.
Preferably, the gas permeable end of the gas permeable unit is connected with a detachable gas permeable plug or a detachable gas permeable membrane with a hydrophobic gas permeable function, namely a hydrophobic gas permeable element, a first valve is arranged at the connecting part of the gas permeable end and the hydrophobic gas permeable element, a second valve is arranged at the connecting part of the gas permeable unit and the chromatographic column, and when gas enters the pipeline, an operator can avoid the gas from entering the chromatographic column by adjusting the valve of the gas permeable unit.
To sum up, owing to adopted above-mentioned technical scheme, the beneficial effects of the utility model are that:
1. the utility model discloses in, under the effect of three-way valve, can realize that the pump pressure pipe only communicates with the syringe or the pump pressure pipe only communicates with the reagent bottle, and the reagent bottle appearance of going up belongs to bulky, the appearance of going up of low accuracy, and the customs adjusts the three-way valve and changes the appearance mode of going up, and operating personnel can be according to the needs of different specification chromatographic columns to the reagent volume of inflow pipeline.
2. The utility model discloses in, the pump pressure pipe mainly plays the effect that produces the pump pressure, and is great than conventional pipe in this selected pump pressure pipe internal diameter, occupies the pipeline space, and consequently the length setting of pump pressure pipe is in order to reach and produce suitable pump pressure, practices thrift this purpose in pipeline space.
3. The utility model discloses in, the structure is comparatively simple, each composition, like conventional pipe, pumping pressure pipe, peristaltic pump, chromatography column, three-way valve, reagent bottle, syringe etc. all can purchase low price market standard spare to it is lower to make this simple and easy purification protein pipeline cost, simple structure, easily operation.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a schematic structural view of the ventilation unit of the present invention.
The labels in the figure are: 2-conventional conduit, 3-pump pressure conduit, 4-peristaltic pump, 5-gas permeable unit, 51-inlet end, 52-gas permeable end, 521-hydrophobic gas permeable element, 522-first valve, 53-outlet end, 531-second valve, 6-chromatographic column, 11-three-way valve, 12-reagent bottle and 13-injector.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention.
A simple protein purification pipeline is shown in figure 1, and comprises a sample loading unit, a conventional conduit 2, a pump pressure conduit 3, a peristaltic pump 4, an air permeable unit 5 and a chromatography column 6, wherein one end of the conventional conduit 2 is connected with the sample loading unit, the other end of the conventional conduit is connected with the peristaltic pump 4, the pump pressure conduit 3 passes through the peristaltic pump 4 to be connected with an inlet end 51 of the air permeable unit 5, and an outlet end 52 of the air permeable unit 5 is connected with the chromatography column 6.
The sample loading unit comprises a three-way valve 11, a reagent bottle 12 and an injector 13, wherein the first end of the three-way valve 11 is connected with the pumping pressure conduit 3, the second end of the three-way valve is connected with the injector 13, the third end of the three-way valve is connected with the reagent bottle 12 through a conventional conduit 2, and the pumping pressure conduit 3 can be only communicated with the injector 13 or the pumping pressure conduit 3 can be only communicated with the reagent bottle 12 under the action of the three-way valve 11. The operator can select to use a syringe to load the sample or a reagent bottle to load the sample according to the specification, the sample loading volume or the reagent capacity of the chromatographic column 6.
The ventilating unit 5 is made of transparent material and is horizontal and vertical, the top of the ventilating unit is provided with an inlet end 51 and a ventilating end 52, and the bottom of the ventilating unit is provided with an outlet end 53.
The length of the pumping pressure conduit 3 is the length required by the function of the peristaltic pump 4, the connectors are arranged at the two ends of the pumping pressure conduit, and an operator can control the required pumping pressure by adjusting the rotating speed of the peristaltic pump 4 and replacing the pumping pressure conduits 3 with different calibers.
The ventilating unit 5 is made of transparent material and is vertical to the horizontal direction, the top of the ventilating unit is provided with an inlet end 52 and a ventilating end 52, and the bottom of the ventilating unit is provided with an outlet end 53.
The hydrophobic air-permeable element 521 connected to the air-permeable end 52 of the air-permeable unit 5 is a detachable air-permeable plug or air-permeable membrane with hydrophobic air-permeable function.
The gas permeable end 52 of the gas permeable unit 5 is connected with a detachable gas permeable plug or a gas permeable membrane with a hydrophobic gas permeable function, namely a hydrophobic gas permeable element 521, a first valve 522 is arranged at the connection part of the gas permeable end 52 and the hydrophobic gas permeable element 521, a second valve 531 is arranged at the connection part of the gas permeable unit 5 and the chromatographic column 6, and when gas enters a pipeline, an operator can prevent the gas from entering the chromatographic column by adjusting the valve of the gas permeable unit 5.
When the peristaltic pump 4 is started, self-suction force is generated inside the pipeline, so that the solution stored in the reagent bottle 12 or the solution in the injector 13 enters the pipeline through the third end or the second end of the three-way valve 11 respectively, flows through the pump pressure conduit 3, enters the chromatographic column 6 through the air-permeable unit 5, and flows out of the outlet end of the chromatographic column 6. The operator may collect the effluent solution using a collector (e.g., centrifuge tube) or the like.
By means of the three-way valve 11 it is possible to achieve that the pump pressure conduit 3 communicates only with the syringe 13 or that the pump pressure conduit 3 communicates only with the reagent bottle 12. An operator can adjust the volume of the reagent flowing into the pipeline according to chromatographic columns 6 with different specifications, and the sample loading mode is changed by adjusting the three-way valve 11, wherein the sample loading of the reagent bottle belongs to the sample loading mode with large volume and low precision; the injector is provided with scales, the sample loading of the injector belongs to a sample loading mode with a small and medium volume and a certain precision, and more reagents are required to be stored in the reagent bottle so as to avoid the phenomenon that the air enters the pipeline due to the fact that the reagents are completely sucked.
The peristaltic pump is characterized in that the pumping pressure conduit 3 penetrates through the peristaltic pump 4, the length of the pumping pressure conduit 3 is the length required by the function of the peristaltic pump 3, joints are arranged at two ends of the pumping pressure conduit, an operator can control the required pumping pressure by adjusting the rotating speed of the peristaltic pump 4 and replacing the pumping pressure conduit 3 with different calibers, the pumping pressure conduit is not required to be overlong and thick, reagent residues and pipeline volume are easily caused to be overlarge, and therefore overlong is not required, the peristaltic pump can be enabled to be connected with the pump head and normally peristalsis can be achieved.
The conventional conduit of the protein purification pipeline plays a role in conveying the reagent, the pumping pressure conduit 3 mainly plays a role in generating pumping pressure, and the inner diameter of the pumping pressure conduit 3 in the selected embodiment is larger than that of the conventional conduit, so that the pumping pressure conduit occupies a pipeline space. The length of the pumping pressure conduit 3 is thus set for the purpose of generating a suitable pumping pressure, saving piping space.
As shown in fig. 2, the ventilation unit 5 is made of transparent material and is vertical to the horizontal direction. The top of which is provided with an inlet end 51 and a gas permeable end 52 and the bottom of which is provided with an outlet end 53. The air permeable end 52 is connected with a hydrophobic air permeable element 521; and a second valve 531 is arranged at the connecting part of the ventilation unit 5 and the chromatographic column 6. When the pipeline is in normal circulation, the second valve 531 at the connection part between the outlet end 51 of the air-permeable unit and the chromatographic column 6 is in an open state; when gas enters the pipeline, it can be observed from the transparent gas permeable unit 5, and the second valve 531 at the connection part of the outlet end 53 of the gas permeable unit and the chromatographic column 6 is closed, and the first valve 522 is opened to remove the gas in the pipeline through the gas permeable end 52 of the gas permeable unit 5, so as to prevent the gas from entering the chromatographic column 6.
The components of the embodiments of the present invention, generally described and illustrated in the figures herein, can be combined and arranged in a variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention.
It should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "connected" and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The particular connections described in selected embodiments of the present invention are for illustrative purposes only and do not imply that modifications and variations to the connections may not be possible in other embodiments.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only. The terms "vertical," "horizontal," "left," "right," "first," "second," and the like are used herein for descriptive purposes only and not for purposes of limitation. As used herein, "and/or" includes any and all combinations of one or more of the associated listed items.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (6)
1. A simple purified protein pipeline is characterized in that: the protein purification pipeline comprises a sample loading unit, a conventional conduit (2), a pumping pressure conduit (3), a peristaltic pump (4), an air permeable unit (5) and a chromatographic column (6), wherein one end of the conventional conduit (2) is connected with the sample loading unit, the other end of the conventional conduit is connected with the peristaltic pump (4), the pumping pressure conduit (3) penetrates through the peristaltic pump (4) to be connected with an inlet end (51) of the air permeable unit (5), and an outlet end (53) of the air permeable unit (5) is connected with the chromatographic column (6).
2. The simple purified protein pipeline as claimed in claim 1, wherein the sample loading unit comprises a three-way valve (11), a reagent bottle (12) and a syringe (13), the first end of the three-way valve (11) is connected with the pumping pressure conduit (3), the second end is connected with the syringe (13), and the third end is connected with the reagent bottle (12) through a conventional conduit (2).
3. The simplified purified protein pipeline as claimed in claim 1, wherein said air-permeable unit (5) is made of transparent material and is horizontally vertical, and has an inlet end (51) and an air-permeable end (52) at the top and an outlet end (53) at the bottom.
4. The simple purified protein pipeline as claimed in claim 3, wherein the air-permeable end (52) of the air-permeable unit (5) is connected with a hydrophobic air-permeable element (521), and the hydrophobic air-permeable element (521) is an air-permeable plug or an air-permeable membrane.
5. The simple purified protein pipeline as claimed in claim 3, wherein a first valve (522) is provided at the connection part of the air-permeable end (52) of the air-permeable unit (5) and the hydrophobic air-permeable element (521), and a second valve (531) is provided at the connection part of the outlet end (53) of the air-permeable unit (5) and the chromatographic column (6).
6. The simple purified protein pipeline as claimed in claim 1, wherein the pumping pressure conduit (3) is a hose with connectors at both ends.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021735041.2U CN213842814U (en) | 2020-08-19 | 2020-08-19 | Simple and easy purified protein pipeline |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021735041.2U CN213842814U (en) | 2020-08-19 | 2020-08-19 | Simple and easy purified protein pipeline |
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| Publication Number | Publication Date |
|---|---|
| CN213842814U true CN213842814U (en) | 2021-07-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021735041.2U Active CN213842814U (en) | 2020-08-19 | 2020-08-19 | Simple and easy purified protein pipeline |
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| CN (1) | CN213842814U (en) |
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2020
- 2020-08-19 CN CN202021735041.2U patent/CN213842814U/en active Active
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