CN213713726U

CN213713726U - Dry save set of target plate and spotting machine

Info

Publication number: CN213713726U
Application number: CN202022016506.5U
Authority: CN
Inventors: 汪云飞; 潘能科
Original assignee: Chongqing Topu Bioengineering Co Ltd
Current assignee: Chongqing Topu Bioengineering Co Ltd
Priority date: 2020-09-15
Filing date: 2020-09-15
Publication date: 2021-07-16
Anticipated expiration: 2030-09-15

Abstract

The utility model relates to a sample pretreatment discloses a dry save set of target plate and some model machine, and this dry save set of target plate includes target plate platform, base, support, closing plate, vacuum pump and cam mechanism, target plate platform and support set up on the base, the target plate platform is including the target groove that is used for placing the target plate and the drive mechanism of drive target plate platform motion, closing plate, vacuum pump and cam mechanism set up on the support, be provided with the opening that corresponds with cam mechanism position on the support, cam mechanism acts on the closing plate so that closing plate and target plate platform form sealed space, the vacuum pump is used for extracting air in the sealed space to reach the purpose of dry target plate. The utility model discloses a set up the lift that the cam structure controlled the closing plate, can make the sealed cabin remain stable encapsulated situation better.

Description

Dry save set of target plate and spotting machine

Technical Field

The utility model relates to a sample pretreatment, more specifically say, relate to a dry save set of target plate and some model machine.

Background

The operating principle of MALDI-TOF-MS is that matrix-assisted laser desorption ionization is utilized to analyze components on the surface of a sample to be detected into ions, and the ions with different mass-to-charge ratios (m/z) are separated through a flight time mass analyzer according to the length of the flight time to form mass spectrum peaks (mass spectrograms) with different intensities. In the field of microorganism identification, a bacterial or fungal mass spectrum database is established by using a standard strain and a strain with clear source typing, and the mass spectrum of an unknown strain is compared with the database by using MassLynx software (or other related software), so that the species of a microorganism to be detected can be determined. Compared with other microorganism detection technologies, the MALDI-TOF-MS technology has obvious high efficiency and accuracy, and the cost of each experiment is very low, so that the MALDI-TOF-MS technology becomes a popular method for microorganism identification.

MALDI-TOF mass spectrometry consists essentially of two parts: matrix-assisted laser desorption ionization ion source (MALDI) and time-of-flight mass analyzer (TOF). The matrix-assisted laser desorption ion source is used for desorbing a solid sample, the sample is manufactured under normal pressure to form a solid, the solid enters a vacuum system through a sample injection mechanism, and the solid is accurately positioned by a positioning mechanism so that the sample is desorbed in a specific area. During the analysis process, the laser provides high energy to the sample in a very short time, and the sample is heated very quickly, so that the matrix molecules in the ion source can effectively absorb the energy of the laser and indirectly transfer the energy to the sample molecules, the sample molecules are ionized, and then the ions enter a detector under the action of high pressure for detection. When a current sample point machine is used for drying a target plate in vacuum, a sealing plate is pressed downwards by virtue of the acting force of a spring, and the acting force of the spring is unstable so that a sealing space in an ideal state cannot be formed, so that the efficiency and the quality of vacuum drying are influenced, and the accuracy of detection is further influenced.

SUMMERY OF THE UTILITY MODEL

Not enough to prior art, the utility model provides a dry save set of target plate and some model machine to the microorganism in the solution environment adheres to the problem of influencing the testing result on the target plate.

In order to achieve the above object, one technical solution adopted in the embodiments of the present application is: the utility model provides a dry save set of target plate, dry save set includes target plate platform, base, support, closing plate, vacuum pump and cam mechanism, target plate platform and support set up on the base, the target plate platform is including the target groove that is used for placing the target plate and the drive mechanism of drive target plate platform motion, closing plate, vacuum pump and cam mechanism set up on the support, be provided with the opening that corresponds with the cam mechanism position on the support, cam mechanism acts on the closing plate, and the closing plate forms confined space with the target plate platform, the vacuum pump is used for extracting the interior air of confined space.

Preferably, the cam structure includes a cam, a motor and a cam mounting bracket, the cam and the motor are disposed on the cam mounting bracket, and the cam is connected to a rotating shaft of the motor.

Preferably, the cam is provided with a distal angle of repose of 20-40 °.

Preferably, a bearing is provided at the maximum stroke of the cam.

Preferably, the cam structure further includes a sensor, the sensor includes a trigger piece and an inductive switch, the trigger piece is disposed at the bottom of the cam, and the inductive switch is disposed on the cam mounting frame and is adapted to the trigger piece.

Preferably, the sealing plate is mounted on the bracket through a threaded rod, a spring is sleeved on the threaded rod, and the sealing plate is pressed on the bracket through the elastic force of the spring.

Preferably, the springs and the threaded rods are 4 in number and are respectively arranged at four corners of the sealing plate.

Preferably, the sealing plate is provided with a trapezoidal groove, and an O ring is arranged in the trapezoidal groove; or a trapezoidal groove is formed in the target groove, and an O ring is installed in the trapezoidal groove.

Preferably, the sealing plate further comprises two electromagnetic valves, the sealing plate is provided with a threaded hole, a quick-screwing straight-inserting connector is installed on the threaded hole, one electromagnetic valve is connected with the air pumping hole of the vacuum pump, the other electromagnetic valve is connected with a three-way connector, and the other two holes of the three-way connector are respectively connected with the air inlet of the vacuum pump and the quick-screwing straight-inserting connector.

A spotting machine comprises the target plate drying and storing device.

The utility model provides a dry save set of target plate, dry save method of target plate and some model machine has following beneficial effect:

(1) the utility model discloses compare in current some model machines, when the target plate vacuum drying, adopt the cam structure to control the lift of closing plate, to the friction damage of O circle when having avoided advancing the cabin, improved O circle life greatly. Meanwhile, the existence of the cam structure can enable the sealed cabin to keep a sealed state more stably, and the vacuum environment has better stability by matching with the sealing on the gas circuit, so that the target plate after the point sample drying is finished can be stored for a longer time.

(2) The utility model discloses an install sealed O circle on the closing plate that is located the top, can effectively prevent the deposition, and then delay the ageing process of O circle, further improved the life of O circle.

(3) The utility model discloses an add the small bearing in the biggest stroke department of cam, can replace rolling friction with the sliding friction between cam and the closing plate, reduced the wearing and tearing of closing plate, improved the control of motor to cam rotation angle simultaneously.

Drawings

Fig. 1 is a schematic structural diagram of a sample counting machine of the utility model;

FIG. 2 is a schematic structural view of the target plate drying and storing device of the present invention;

fig. 3 is a schematic view of the connection and operation mode structure of the vacuum pump of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the following drawings and examples, which are illustrative only and not limiting, and the scope of the present invention should not be limited thereby.

It is to be understood that, unless otherwise expressly stated or limited, the terms "disposed," "mounted," "connected," and the like are intended to be used in a generic sense, such as "fixedly connected," removably connected, or integrated; the connection can be mechanical connection or electrical connection; the connection may be direct or indirect via an intermediate medium, and may be communication between the two elements or an interaction relationship between the two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Example 1:

as shown in fig. 1, 2 and 3, the target plate drying and storing device includes a target plate platform 1, a base 2, a bracket 3, a sealing plate 4, an oil pump 5, a first switch 6, a second switch 7, a cam mechanism 8, a target groove 11, a transmission mechanism 12, an opening 31, a quick-screwing straight plug 41, a spring 42, a threaded rod 43, a trigger 841 and an electro-optical switch 842.

Target plate platform 1 and support 3 set up on base 2, closing plate 4 and cam mechanism all install on support 3, opening 31 has been seted up on support 3, opening 31's position is corresponding with closing plate 4 center, target plate platform 1 includes target groove 11 and drive mechanism 12, and target groove 11 slightly is greater than the target plate, and the top is seted up slottedly and is used for placing the target plate, and the inslot is provided with a magnet simultaneously for the target plate of putting into the inslot is fixed, has still seted up two darker holes on the target groove 11, makes things convenient for the finger to stretch into and gets and put the target plate. The driving mechanism 12 controls the movement of the target plate platform 1, and when the target plate platform 1 moves to the lower part of the sealing plate 4, the sealing plate 4 moves downwards to cover the target slot 11 to form a sealed space.

Have dovetail groove, screw hole on the closing plate 4, still include spring 42 and threaded rod 43, the dovetail groove is used for installing O circle, and the screw hole is used for the installation to twist directly inserting joint 41 soon, and spring 42 and threaded rod 43 are all four, and closing plate 4 passes through threaded rod 43 to be installed on the support, and the cover is equipped with spring 42 on the threaded rod 43, and spring 42's elasticity compresses tightly closing plate 4 on the support.

The oil pump 5 is used as a vacuum pump, and the components to be dried include water (saturated vapor pressure at room temperature is about 3100Pa), formic acid (saturated vapor pressure at room temperature is about 5330Pa), and alcohol (saturated vapor pressure at room temperature is about 8500Pa), and the ultimate vacuum that the oil pump 5 can achieve is less than 10Pa, which can accelerate the drying speed of the target plate. One connector of a first switch (a two-position two-way electromagnetic valve) 6 is connected with an air suction port of the oil pump 5 through a hard Teflon pipe (a hard pipe for preventing the pipeline from being flattened by atmospheric pressure during air suction), and the other connector is connected with the atmosphere; one connector of a second switch (a two-position two-way electromagnetic valve) 7 is connected with the atmosphere, the other connector is connected with a three-way joint, the other two ports of the three-way joint are a quick-screwing straight-inserting joint 41 connected with a sealing plate 4, and the other one is connected with an air outlet of an oil pump 5. When the vacuum is pumped, the first switch 6 is turned on, and the second switch 7 is turned off; when the air is discharged, the second switch 7 is opened, and the first switch 6 is closed; when the storage is carried out, the first switch and the second switch are both closed.

The cam mechanism 8 comprises a cam 81, a speed reducing motor 82, a cam mounting bracket 83 and a sensor 84, wherein the cam mounting bracket 83 is arranged on a support 83, the cam 81 and the speed reducing motor 82 are installed on the cam mounting bracket 83, the shape of the cam 81 is an ellipse, the position of the cam 81 is matched with that of an opening 31 on the support (the cam 81 is positioned right above the opening 31 of the support), the cam 81 penetrates through the opening 31 and pushes the sealing plate 4 downwards when rotating, an O ring on the sealing plate 4 is pressed on the target groove 12 to form a sealed space, and in addition, in order to prevent the rotation angle of the motor from deviating within a certain range, the cam 81 is provided with a larger remote repose angle (about. The reduction motor 82 is directly connected to the cam 81 to provide a large torque and a slow speed, thereby making it easier to control the angle of rotation. The sensor 84 includes a trigger piece 841 and a photoelectric switch 842, wherein the trigger piece 841 is disposed on the cam 81, the photoelectric switch 842 is disposed on the cam mounting bracket 83, when the cam 81 is driven by the speed-reducing motor 82 to press the sealing plate 4 downwards to reach the maximum stroke (the maximum stroke mentioned here refers to the position where the cam edge is farthest away from the rotation center), the trigger piece 841 on the cam 81 is matched with the photoelectric switch 842, and the opening and closing state of the sealed space is judged by detecting the photoelectric signal trigger piece 841.

The printer shown in fig. 1 comprises the target plate dry storage device.

The working process of the target plate drying and storing device is as follows:

(1) after the sample applicator of the utility model is powered on, the target plate after sample application is put into the target groove 11 of the target plate platform 1, the sample applicator can start the drying function according to the instruction, and the target plate platform 1 moves to the lower part of the sealing plate 4 under the driving of the transmission mechanism 12;

(2) the cam 81 is driven by the speed reducing motor 82 to rotate, when the cam 81 moves to be vertical to the plane of the sealing plate 4, the cam 81 presses the sealing plate 4 downwards through the opening 31 to reach the maximum stroke, a sealing space is formed between the sealing plate and the target slot 11, the trigger piece 841 cuts off a signal of the photoelectric switch 842 at the moment, and the control system judges that the sealing space is in a closed state;

(3) the control system controls the oil pump 5 to work, the first switch 6 is turned on, the second switch 7 is turned off, air in the sealed space is pumped out to form a vacuum sealed space, and the target plate is dried;

(4) after the target plate is dried, the control system further controls the first switch 6 to be closed, the second switch 7 to be opened, air enters the sealed space until the air pressure is balanced, the cam 81 is driven by the speed reducing motor 82 to rotate until the longest radius line deviates from the vertical line of the sealing plate 4, the sealing plate 4 is pushed against the support 3 under the action of the spring 42, the photoelectric switch 842 is recovered to be light-on, and the control system judges that the sealed space is in an open state;

(5) when the target plate needs to be stored in the drying and storing device, the control system controls the first switch 6 and the second switch 7 to be turned off.

(6) The target plate platform 1 moves to a zero position under the driving of the transmission mechanism 12, and the target plate is taken out.

Example 2

The difference between this embodiment and embodiment 1 is that the sealing plate 4 does not have a trapezoidal groove, and the O-ring is not mounted on the sealing plate, but on the target groove 11, and the O-ring has a useful life, which is usually not so long, and is easy to replace when mounted on the target groove.

Example 3

The embodiment is different from embodiment 1 in that a small bearing is provided at a position where the cam 81 passes through the opening 31 to press down the sealing plate 4 to the maximum stroke, and by adding the small bearing at the maximum stroke of the cam 81, sliding friction between the cam 81 and the sealing plate 4 can be replaced by rolling friction, wear of the sealing plate 4 is reduced, and control of the rotation angle of the cam 81 by the motor is improved.

The above embodiments are merely illustrative, and not restrictive, of the present invention. Changes, modifications, etc. to the above-described embodiments are intended to fall within the scope of the claims of the present invention, as long as they are in accordance with the technical spirit of the present invention.

Claims

1. The utility model provides a dry save set of target plate, its characterized in that, dry save set includes target plate platform, base, support, closing plate, vacuum pump and cam mechanism, target plate platform and support set up on the base, the target plate platform is including the target groove that is used for placing the target plate and the drive mechanism of drive target plate platform motion, closing plate, vacuum pump and cam mechanism set up on the support, be provided with the opening that corresponds with the cam mechanism position on the support, cam mechanism acts on the closing plate, and the closing plate forms confined space with the target plate platform, the vacuum pump is used for extracting air in the confined space.

2. The target board drying and preserving device of claim 1, wherein the cam mechanism comprises a cam, a motor and a cam mounting bracket, the cam and the motor are arranged on the cam mounting bracket, and the cam is connected with a rotating shaft of the motor.

3. The target board dry storage device of claim 2, wherein the cam has a distal angle of repose of 20-40 °.

4. The target board dry storage device of claim 2, wherein a bearing is provided at the maximum stroke of the cam.

5. The dry target board storage device of claim 2, wherein the cam mechanism further comprises a sensor, the sensor comprises a trigger piece and a sensor switch, the trigger piece is disposed at the bottom of the cam, and the sensor switch is disposed on the cam mounting bracket and is adapted to the trigger piece.

6. The dry target plate storage device of any one of claims 1 to 5, wherein the sealing plate is mounted on the bracket by a threaded rod, and a spring is sleeved on the threaded rod, and the elastic force of the spring presses the sealing plate against the bracket.

7. The dry storage device for target plates of claim 6, wherein 4 springs and 4 threaded rods are disposed at four corners of the sealing plate.

8. The dry target plate storage device of claim 6, wherein the sealing plate is provided with a trapezoidal groove, and an O-ring is arranged in the trapezoidal groove; or a trapezoidal groove is formed in the target groove, and an O ring is installed in the trapezoidal groove.

9. The dry target plate storage device of claim 6, further comprising two solenoid valves, wherein the sealing plate has a threaded hole, and a fast screw direct plug is mounted on the threaded hole, wherein one of the solenoid valves is connected to the suction port of the vacuum pump, and the other solenoid valve is connected to a three-way joint, and the other two ports of the three-way joint are respectively connected to the air inlet of the vacuum pump and the fast screw direct plug.

10. Printer characterized in that it comprises a dry storage device for target plates according to any of claims 1-9.