CN213506887U - Sample preprocessing device for gene sequencing - Google Patents
Sample preprocessing device for gene sequencing Download PDFInfo
- Publication number
- CN213506887U CN213506887U CN202022376800.7U CN202022376800U CN213506887U CN 213506887 U CN213506887 U CN 213506887U CN 202022376800 U CN202022376800 U CN 202022376800U CN 213506887 U CN213506887 U CN 213506887U
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- China
- Prior art keywords
- fixedly connected
- pump
- sample
- gene sequencing
- pretreating
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 34
- 238000012163 sequencing technique Methods 0.000 title claims abstract description 29
- 238000007781 pre-processing Methods 0.000 title description 6
- 238000007789 sealing Methods 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 210000003855 cell nucleus Anatomy 0.000 abstract description 13
- 238000005119 centrifugation Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000006399 behavior Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Abstract
The utility model discloses a sample pretreatment device for gene sequencing, which comprises a body, wherein the inner bottom of the body is fixedly connected with a first pump, the output end of the first pump is fixedly connected with a connecting pipe, and one end of the connecting pipe, which is far away from the first pump, is fixedly connected with a sealing cover; the inner bottom of the body is fixedly connected with a centrifugal machine, the inner side wall of the sealing cover is fixedly connected with a first sealing ring, and the inner part of the first sealing ring is attached to the outer surface of the centrifugal machine; the telescopic motor is fixedly connected to the inner top of the body, a telescopic rod is arranged at the output end of the telescopic motor, a connecting plate is fixedly connected to one end, far away from the telescopic motor, of the telescopic rod, and a second pump is fixedly connected to the lower surface of the connecting plate. The utility model discloses, rational in infrastructure, can effectually separate the cell nucleus, can be simultaneously with the leading-in sample board that goes of quantitative cell nucleus on, be convenient for carry out many times equivalent to the liquid after the centrifugation and take a sample.
Description
Technical Field
The utility model relates to a gene sequencing sample pretreatment field especially relates to a sample preprocessing device for gene sequencing.
Background
Gene sequencing is a novel gene detection technology, can analyze and determine the complete sequence of genes from blood or saliva, and predicts the possibility of suffering from various diseases, and the behavior characteristics and behaviors of individuals are reasonable.
The gene sequencing technology can lock the individual pathological change gene and prevent and treat in advance.
The conventional pretreatment of a gene sequencing sample needs to extract cell nucleuses in cells, so that required DNA chains are obtained inside the cell nucleuses, and then genes are sequenced.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a sample pretreatment device for gene sequencing.
In order to achieve the above purpose, the utility model adopts the following technical scheme: a sample pretreatment device for gene sequencing comprises a body, wherein a first pump is fixedly connected to the inner bottom of the body, a connecting pipe is fixedly connected to the output end of the first pump, and a sealing cover is fixedly connected to one end, far away from the first pump, of the connecting pipe;
the inner bottom of the body is fixedly connected with a centrifugal machine, the inner side wall of the sealing cover is fixedly connected with a first sealing ring, and the inner part of the first sealing ring is attached to the outer surface of the centrifugal machine;
a telescopic motor is fixedly connected to the inner top of the body, a telescopic rod is arranged at the output end of the telescopic motor, a connecting plate is fixedly connected to one end, away from the telescopic motor, of the telescopic rod, and a second pump is fixedly connected to the lower surface of the connecting plate;
the output end of the second pump is fixedly connected with an output pipe, and one end, far away from the second pump, of the output pipe is fixedly connected with a liquid dropping port.
As a further description of the above technical solution:
the input end fixedly connected with suction tube of second pump machine, the one end fixedly connected with that the second pump machine was kept away from to the suction tube gets the liquid piece, the last fixed surface who gets the liquid piece is connected with the second sealing washer.
As a further description of the above technical solution:
the inner top of the sealing cover is provided with a connecting groove, and the position of the connecting groove corresponds to the position of the second sealing ring.
As a further description of the above technical solution:
the body is to the inside wall fixedly connected with stopper, the spacing groove has been seted up to the inside of stopper, and the inner wall of spacing groove and the surface of output tube laminate mutually.
As a further description of the above technical solution:
the inner bottom of the body is fixedly connected with a placing table, and the placing table is positioned below the liquid dropping port.
As a further description of the above technical solution:
the side wall of the body is provided with a placing opening, and the position of the placing opening corresponds to the position of the placing table.
As a further description of the above technical solution:
the input fixedly connected with feed liquor pipe of first pump machine, and the surface of feed liquor pipe and the lateral wall fixed connection of body.
As a further description of the above technical solution:
the top of the sealing cover is provided with a connector, and the inner wall of the connector is attached to the outer surface of the suction pipe.
The utility model discloses following beneficial effect has:
1. compared with the prior art, the sample pretreatment device for gene sequencing performs fractional centrifugation on the broken cells through the centrifugal machine, so that the device can conveniently obtain substances such as cell nucleuses and the like in the lower-layer centrifugate, and the device can conveniently obtain genes of the cells.
2. Compared with the prior art, this sample preprocessing device for gene sequencing makes the device can be free add cell liquid and pure water toward centrifuge inside through first pump for the cell can be inside the device accomplish the water absorption and expand brokenly, reduces the transfer time, is convenient for guarantee the activity of cell.
3. Compared with the prior art, the sample pretreatment device for gene sequencing enables the device to suck the centrifuged liquid through the second pump, so that the liquid containing a large number of cell nucleuses is obtained, and DNA is obtained for gene sequencing.
4. Compared with the prior art, this sample preprocessing device for gene sequencing makes the device can control the content of once getting liquid through the drip mouth, is convenient for carry out the sample of many copies equivalent with the liquid after the centrifugation for the device once separates and can obtain many copies of sample and carry out gene sequencing.
Drawings
FIG. 1 is a front view of the internal structure of a sample pretreatment device for gene sequencing according to the present invention;
fig. 2 is an enlarged view of a structure at a position a in fig. 1 according to the present invention;
FIG. 3 is a front view of the overall structure of a sample pretreatment device for gene sequencing according to the present invention;
FIG. 4 is a schematic diagram of the overall structure of a sample pretreatment device for gene sequencing according to the present invention.
Illustration of the drawings:
1. a body; 2. a first pump; 3. a liquid inlet pipe; 4. a connecting pipe; 5. a sealing cover; 6. a centrifuge; 7. a first seal ring; 8. a telescopic motor; 9. a telescopic rod; 10. a connecting plate; 11. a second pump; 12. an extraction tube; 13. taking a liquid block; 14. a second seal ring; 15. connecting grooves; 16. an output pipe; 17. a limiting block; 18. a limiting groove; 19. a drip port; 20. a placing table; 21. a placement port; 22. and (7) connecting ports.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1-4, the utility model provides a sample preprocessing device for gene sequencing: the device comprises a body 1, wherein a first pump 2 is fixedly connected to the inner bottom of the body 1, a liquid inlet pipe 3 is fixedly connected to the input end of the first pump 2, the outer surface of the liquid inlet pipe 3 is fixedly connected with the side wall of the body 1, a connecting pipe 4 is fixedly connected to the output end of the first pump 2, and a sealing cover 5 is fixedly connected to one end, far away from the first pump 2, of the connecting pipe 4; the centrifuge 6 is fixedly connected to the inner bottom of the body 1, the first sealing ring 7 is fixedly connected to the inner side wall of the sealing cover 5, and the inner part of the first sealing ring 7 is attached to the outer surface of the centrifuge 6, so that the first pump 2 can conveniently add cell sap and purified water into the centrifuge 6 to enable cells to be subjected to expansion breaking and then centrifugation;
a telescopic motor 8 is fixedly connected to the inner top of the body 1, a telescopic rod 9 is arranged at the output end of the telescopic motor 8, a connecting plate 10 is fixedly connected to one end, away from the telescopic motor 8, of the telescopic rod 9, and a second pump 11 is fixedly connected to the lower surface of the connecting plate 10, so that cell nucleuses can be conveniently extracted;
input fixedly connected with suction tube 12 of second pump machine 11, suction tube 12 keeps away from the one end fixedly connected with of second pump machine 11 and gets liquid piece 13, get the last fixed surface of liquid piece 13 and be connected with second sealing washer 14, spread groove 15 has been seted up at the interior top of sealed lid 5, and spread groove 15's position is corresponding with the position of second sealing washer 14, the output fixedly connected with output tube 16 of second pump machine 11, the one end fixedly connected with drip mouth 19 of second pump machine 11 is kept away from to output tube 16, be convenient for take out the cell nucleus.
The working principle is as follows: during the use, add cell sap through first pump machine 2 to centrifuge 6 inside, later add appropriate amount of pure water for the cell can absorb water and expand brokenly, and subsequent centrifugal separation of being convenient for rotates through centrifuge 6 and makes the cell nucleus part can separate with cytoplasm etc. absorbs through second pump machine 11 cell nucleus after will separating, and drip the cell nucleus after will separating on the sampling plate through drip 19, accomplish the preliminary treatment to the cell nucleus.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (8)
1. A sample pretreatment device for gene sequencing, comprising a body (1), characterized in that: the inner bottom of the body (1) is fixedly connected with a first pump (2), the output end of the first pump (2) is fixedly connected with a connecting pipe (4), and one end, far away from the first pump (2), of the connecting pipe (4) is fixedly connected with a sealing cover (5);
the inner bottom of the body (1) is fixedly connected with a centrifugal machine (6), the inner side wall of the sealing cover (5) is fixedly connected with a first sealing ring (7), and the interior of the first sealing ring (7) is attached to the outer surface of the centrifugal machine (6);
a telescopic motor (8) is fixedly connected to the inner top of the body (1), a telescopic rod (9) is arranged at the output end of the telescopic motor (8), a connecting plate (10) is fixedly connected to one end, far away from the telescopic motor (8), of the telescopic rod (9), and a second pump (11) is fixedly connected to the lower surface of the connecting plate (10);
the output end of the second pump (11) is fixedly connected with an output pipe (16), and one end, far away from the second pump (11), of the output pipe (16) is fixedly connected with a liquid dropping port (19).
2. The apparatus for pretreating a sample for gene sequencing according to claim 1, wherein: the input fixedly connected with suction tube (12) of second pump machine (11), the one end fixedly connected with of second pump machine (11) is kept away from in suction tube (12) gets liquid piece (13), the last fixed surface who gets liquid piece (13) is connected with second sealing washer (14).
3. The apparatus for pretreating a sample for gene sequencing according to claim 1, wherein: the inner top of the sealing cover (5) is provided with a connecting groove (15), and the position of the connecting groove (15) corresponds to the position of the second sealing ring (14).
4. The apparatus for pretreating a sample for gene sequencing according to claim 1, wherein: the utility model discloses a joint structure of a motor vehicle, including body (1), inside wall fixedly connected with stopper (17) that body (1) arrived, spacing groove (18) have been seted up to the inside of stopper (17), and the inner wall of spacing groove (18) is laminated mutually with the surface of output tube (16).
5. The apparatus for pretreating a sample for gene sequencing according to claim 1, wherein: the inner bottom of the body (1) is fixedly connected with a placing table (20), and the placing table (20) is positioned below the liquid dropping port (19).
6. The apparatus for pretreating a sample for gene sequencing according to claim 1, wherein: a placing opening (21) is formed in the side wall of the body (1), and the position of the placing opening (21) corresponds to the position of the placing table (20).
7. The apparatus for pretreating a sample for gene sequencing according to claim 1, wherein: the input end of the first pump (2) is fixedly connected with a liquid inlet pipe (3), and the outer surface of the liquid inlet pipe (3) is fixedly connected with the side wall of the body (1).
8. The apparatus for pretreating a sample for gene sequencing according to claim 1, wherein: the top of the sealing cover (5) is provided with a connecting port (22), and the inner wall of the connecting port (22) is attached to the outer surface of the suction pipe (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022376800.7U CN213506887U (en) | 2020-10-23 | 2020-10-23 | Sample preprocessing device for gene sequencing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022376800.7U CN213506887U (en) | 2020-10-23 | 2020-10-23 | Sample preprocessing device for gene sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213506887U true CN213506887U (en) | 2021-06-22 |
Family
ID=76401679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022376800.7U Expired - Fee Related CN213506887U (en) | 2020-10-23 | 2020-10-23 | Sample preprocessing device for gene sequencing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213506887U (en) |
-
2020
- 2020-10-23 CN CN202022376800.7U patent/CN213506887U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210622 |
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| CF01 | Termination of patent right due to non-payment of annual fee |