CN213475992U - PCR thermal cycling device - Google Patents
PCR thermal cycling device Download PDFInfo
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- CN213475992U CN213475992U CN202022156321.4U CN202022156321U CN213475992U CN 213475992 U CN213475992 U CN 213475992U CN 202022156321 U CN202022156321 U CN 202022156321U CN 213475992 U CN213475992 U CN 213475992U
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- 238000005382 thermal cycling Methods 0.000 title claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims abstract description 70
- 239000002184 metal Substances 0.000 claims abstract description 54
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 54
- 238000001816 cooling Methods 0.000 claims abstract description 35
- 238000005485 electric heating Methods 0.000 claims abstract description 27
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000000741 silica gel Substances 0.000 claims abstract description 15
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 15
- 230000017525 heat dissipation Effects 0.000 claims description 11
- 230000005855 radiation Effects 0.000 claims description 8
- 210000005056 cell body Anatomy 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 4
- 230000001351 cycling effect Effects 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 239000000498 cooling water Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The utility model relates to the technical field of PCR and discloses a PCR thermal cycling device, which comprises a thermal cycling box, the bottom of the thermal cycle box is fixedly connected with a water cooling box, the right side of the water cooling box is fixedly connected with a micro pump, the top of the micro pump is fixedly connected with a water delivery pipe, the left side of the water cooling tank is fixedly connected with a water return pipe, the inner side of the thermal cycle box is fixedly connected with a metal heating groove body, the outer side of the metal heating groove body is fixedly connected with an electric heating pipe, the bottom of the metal heating groove body is fixedly connected with a radiating fin, the inner side of the metal heating groove body is fixedly connected with a heat-conducting silica gel gasket, the inner side of the metal heating tank body is movably connected with a sample tank body, the right side of the thermal cycle box is fixedly connected with a driving motor, the output shaft of the driving motor is fixedly connected with a threaded rod, and the outer side of the threaded rod is in threaded connection with a lifting block. The PCR thermal cycling device is uniform in heating and good in thermal cycling effect.
Description
Technical Field
The utility model relates to a PCR technical field specifically is a PCR thermal cycle device.
Background
PCR (polymerase chain reaction) is a molecular biotechnology, and can amplify specific DNA fragments in vitro, and the technology is widely applied to the fields of molecular diagnosis, precise medical treatment, modern agriculture, food safety and the like at present, and in most PCR reactions, the amplification of the specific DNA fragments needs to be realized by periodically heating and cooling samples.
The traditional thermal cycling device heats a sample unevenly, the water bath thermal cycle realizes the heating and cooling processes of the sample by repeatedly soaking the sample in two constant temperature pools of high temperature and low temperature, the equipment is heavy and inconvenient to use, and the conventional metal bath heats the sample unevenly by placing the sample in a reagent tube, so that the PCR thermal cycling device is provided to solve the problem.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
Not enough to prior art, the utility model provides a PCR thermal cycling device possesses the heating evenly, and advantages such as thermal cycle is effectual have solved traditional thermal cycling device heating evenly inadequately, and the not good problem of thermal cycle effect.
(II) technical scheme
For realizing that above-mentioned heating is even, and the effectual purpose of thermal cycle, the utility model provides a following technical scheme: a PCR thermal cycle device comprises a thermal cycle box, wherein the bottom of the thermal cycle box is fixedly connected with a water cooling box, the right side of the water cooling box is fixedly connected with a micropump, the top of the micropump is fixedly connected with a water delivery pipe, the left side of the water cooling box is fixedly connected with a water return pipe, the inner side of the thermal cycle box is fixedly connected with a metal heating groove body, the outer side of the metal heating groove body is fixedly connected with an electric heating pipe, the bottom of the metal heating groove body is fixedly connected with a heat radiation fin, the inner side of the metal heating groove body is fixedly connected with a heat conduction silica gel gasket, the inner side of the metal heating groove body is movably connected with a sample groove body, the right side of the thermal cycle box is fixedly connected with a driving motor, the output shaft of the driving motor is fixedly connected with a threaded rod, the outer side of, the left side fixedly connected with support of thermal cycle case, the top fixedly connected with radiator fan of support, the interior diapire fixedly connected with temperature sensor of metal heating cell body, the left side fixedly connected with controller of thermal cycle case.
Preferably, a water inlet hole is formed in the right side of the thermal circulation box, a water outlet hole is formed in the left side of the thermal circulation box, the top end of the water delivery pipe is fixedly connected with the water inlet hole, and the top end of the water return pipe is fixedly connected with the water outlet hole.
Preferably, the number of the electric heating tubes is four, the four electric heating tubes are arranged on the outer side of the metal heating groove body at equal intervals, the number of the heat dissipation fins is three, and the three heat dissipation fins are arranged on the outer side of the metal heating groove body at equal intervals.
Preferably, the right side of the driving motor is fixedly connected with a guide plate, the lifting block is connected with the guide plate in a sliding mode, the inner side of the thermal cycle box is fixedly connected with a sealing ring, and the sealing ring is tightly attached to the cover plate.
Preferably, the heat-conducting silica gel gasket is located between the sample tank body and the metal heating tank body, and the heat-conducting silica gel gasket is tightly attached to the sample tank body.
Preferably, the micro pump, the electric heating tube, the driving motor, the cooling fan and the temperature sensor are all electrically connected with the controller.
(III) advantageous effects
Compared with the prior art, the utility model provides a PCR thermal cycle device possesses following beneficial effect:
the PCR thermal cycle device comprises a sample tank body, wherein a sample is placed on the inner side of the sample tank body, a metal heating tank body is heated through an electric heating pipe, the electric heating pipe wraps the metal heating tank body in a U shape, the metal heating tank body can be better heated, and then the electric heating pipe is tightly attached to the sample tank body through a heat-conducting silica gel gasket which can be better attached to the sample tank body compared with metal, so that the heat-conducting performance is ensured, the sample tank body can be more uniformly heated in a heat-conducting manner, the heating temperature is monitored through a temperature sensor, when the heating time is enough, the electric heating pipe stops heating, a threaded rod is driven to rotate through a driving motor, a lifting block is driven to move upwards, a cover plate is lifted, a cooling fan is started to drive air to flow, and hot air in a thermal cycle box is driven to move outwards, meanwhile, a micro pump pumps water cooling water in the, the water immersion heat dissipation fins dissipate heat and then return to the water cooling tank from the water return pipe, the flowing water dissipates heat to cool the metal heating tank, so that the metal heating tank can be rapidly cooled, air cooling and water cooling are combined, the cooling efficiency is high, the heat circulation effect is good, and the heat circulation device is uniform in heating and good in heat circulation effect.
Drawings
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is a bottom view of the metal heating tank of the present invention.
In the figure: 1 thermal cycle case, 2 water-cooling case, 3 micropumps, 4 raceway, 5 wet return, 6 metal heating cell body, 7 electrothermal tube, 8 heat dissipation fin, 9 heat conduction silica gel gasket, 10 sample cell body, 11 driving motor, 12 threaded rod, 13 elevator, 14 apron, 15 supports, 16 radiator fan, 17 temperature sensor, 18 controller.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-2, a PCR thermal cycling device comprises a thermal cycling box 1, a water cooling box 2 is fixedly connected to the bottom of the thermal cycling box 1, a micro pump 3 is fixedly connected to the right side of the water cooling box 2, a water delivery pipe 4 is fixedly connected to the top of the micro pump 3, a water return pipe 5 is fixedly connected to the left side of the water cooling box 2, a water inlet is formed in the right side of the thermal cycling box 1, a water outlet is formed in the left side of the thermal cycling box 1, the top end of the water delivery pipe 4 is fixedly connected to the water inlet, the top end of the water return pipe 5 is fixedly connected to the water outlet, a metal heating tank body 6 is fixedly connected to the inner side of the thermal cycling box 1, electric heating pipes 7 are fixedly connected to the outer side of the metal heating tank body 6, the number of the electric heating pipes 7 is four, the four electric heating pipes 7 are equidistantly arranged, the number of the heat dissipation fins 8 is three, the three heat dissipation fins 8 are arranged at equal intervals on the outer side of the metal heating tank body 6, the inner side of the metal heating tank body 6 is fixedly connected with a heat conduction silica gel gasket 9, the inner side of the metal heating tank body 6 is movably connected with a sample tank body 10, the heat conduction silica gel gasket 9 is positioned between the sample tank body 10 and the metal heating tank body 6, the heat conduction silica gel gasket 9 is tightly attached to the sample tank body 10, the right side of the thermal circulation box 1 is fixedly connected with a driving motor 11, the model of the driving motor 11 can be SS4201A55A, the output shaft of the driving motor 11 is fixedly connected with a threaded rod 12, the outer side of the threaded rod 12 is in threaded connection with a lifting block 13, the right side of the driving motor 11 is fixedly connected with a guide plate, the lifting block 13 is in sliding connection with the guide plate, the bottom of the lifting block 13 is, the left side of the thermal circulation box 1 is fixedly connected with a support 15, the top of the support 15 is fixedly connected with a heat radiation fan 16, the type of the heat radiation fan 16 can be AC17251, the inner bottom wall of the metal heating groove body 6 is fixedly connected with a temperature sensor 17, the type of the temperature sensor 17 can be PT100, the left side of the thermal circulation box 1 is fixedly connected with a controller 18, the type of the controller 18 can be FX2N-16MR-001, the micropump 3, the electric heating tube 7, the driving motor 11, the heat radiation fan 16 and the temperature sensor 17 are all electrically connected with the controller 18, each electric appliance element is controlled by the controller 18 to ensure the normal circulation of the thermal circulation, a sample is placed on the inner side of the sample groove body 10, the metal heating groove body 6 is heated by the electric heating tube 7, the metal heating groove body 6 can be better heated because the electric heating tube 7 is wrapped by the metal heating groove body 6 in a U shape, and is tightly, compared with metal, the heat-conducting silica gel gasket 9 can be better attached to the sample tank body 10, so that the heat-conducting performance is ensured, the sample tank body 10 can be more uniformly heated in a heat-conducting manner, the heating temperature is monitored by the temperature sensor 17, when the heating time is enough, the electric heating tube 7 stops heating, the driving motor 11 drives the threaded rod 12 to rotate, so that the lifting block 13 is driven to move upwards, the cover plate 14 is lifted, the heat-radiating fan 16 is started to drive the air to flow, so that the hot air in the heat-circulating box 1 is driven to move outwards, meanwhile, the micro-pump 3 pumps the cooling water in the water-cooling box 2, the cooling water is conveyed to the heat-circulating box 1 through the water conveying pipe 4, the water-soaking heat-radiating fins 8 are used for radiating heat and then returns to the water-cooling box 2 from the water return pipe 5, the flowing water is used for radiating heat to cool the water, so that the, the thermal cycle effect is good.
When the device is used, a sample is placed on the inner side of the sample tank body 10, the metal heating tank body 6 is heated through the electric heating tube 7, the metal heating tank body 6 is wrapped by the electric heating tube 7 in a U shape, the metal heating tank body 6 can be better heated, the electric heating tube 9 is tightly attached to the sample tank body 10 through the heat conduction silica gel gasket 9, the heat conduction silica gel gasket 9 can be better attached to the sample tank body 10 compared with metal, so that the heat conduction performance is ensured, the sample tank body 10 can be more uniformly heated in a heat conduction mode, the heating temperature is monitored through the temperature sensor 17, when the heating time is enough, the electric heating tube 7 stops heating, the driving motor 11 drives the threaded rod 12 to rotate, the lifting block 13 is driven to move upwards, the cover plate 14 is lifted, the cooling fan 16 is started to drive air to flow, so that hot air in the thermal circulation box 1 is driven to move outwards, and meanwhile, the micro pump 3, carry 1 inside thermal cycle case through raceway 4, water logging heat radiation fins 8 dispel the heat, return to water cooling case 2 from wet return 5 again, and the water heat dissipation that flows cools off it, makes metal heating cell body 6 can rapid cooling, and forced air cooling water-cooling combines together, and the cooling efficiency is high, and the thermal cycle is effectual.
To sum up, in the PCR thermal cycling device, a sample is placed inside the sample tank 10, the metal heating tank 6 is heated by the electric heating tube 7, the metal heating tank 6 is better heated by the electric heating tube 7 wrapping the metal heating tank 6 in a U shape, and then the metal heating tank 6 is better heated by the heat conducting silicone gasket 9 tightly attached to the sample tank 10, the heat conducting silicone gasket 9 can better attach to the sample tank 10 compared with metal, so as to ensure heat conducting performance, and also can heat and conduct heat to the sample tank 10 more uniformly, the heating temperature is monitored by the temperature sensor 17, when the heating time is enough, the electric heating tube 7 stops heating, and then the driving motor 11 drives the threaded rod 12 to rotate, so as to drive the lifting block 13 to move upwards, lift the cover plate 14, the heat dissipation fan 16 starts to drive air to flow, so as to drive the hot air inside the thermal cycling box 1 to move outwards, micropump 3 takes out 2 inside cooling waters of water-cooling case, carry 1 inside thermal cycle case through raceway 4, water immersion heat radiation fins 8 dispel the heat, return to water-cooling case 2 from wet return 5 again, the water heat dissipation that flows cools off it, make metal heating cell body 6 can rapid cooling, the forced air cooling water-cooling combines together, the cooling efficiency is high, the thermal cycle is effectual, this thermal cycle device, the heating is even, and the thermal cycle is effectual, it is not even enough to have solved the heating of traditional thermal cycle device, and the not good problem of thermal cycle effect.
It is to be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A PCR thermal cycling device comprises a thermal cycling box (1), and is characterized in that: the bottom of the heat circulation box (1) is fixedly connected with a water cooling box (2), the right side of the water cooling box (2) is fixedly connected with a micropump (3), the top of the micropump (3) is fixedly connected with a water delivery pipe (4), the left side of the water cooling box (2) is fixedly connected with a water return pipe (5), the inner side of the heat circulation box (1) is fixedly connected with a metal heating groove body (6), the outer side of the metal heating groove body (6) is fixedly connected with an electric heating pipe (7), the bottom of the metal heating groove body (6) is fixedly connected with a heat radiation fin (8), the inner side of the metal heating groove body (6) is fixedly connected with a heat conduction silica gel gasket (9), the inner side of the metal heating groove body (6) is movably connected with a sample groove body (10), the right side of the heat circulation box (1) is fixedly connected with a driving motor (11), and the output shaft of, the outside threaded connection of threaded rod (12) has elevator (13), the bottom fixedly connected with apron (14) of elevator (13), left side fixedly connected with support (15) of thermal cycle case (1), the top fixedly connected with radiator fan (16) of support (15), interior diapire fixedly connected with temperature sensor (17) of metal heating cell body (6), left side fixedly connected with controller (18) of thermal cycle case (1).
2. The PCR thermal cycler apparatus of claim 1, wherein: the water inlet hole is formed in the right side of the thermal circulation box (1), the water outlet hole is formed in the left side of the thermal circulation box (1), the top end of the water conveying pipe (4) is fixedly connected with the water inlet hole, and the top end of the water return pipe (5) is fixedly connected with the water outlet hole.
3. The PCR thermal cycler apparatus of claim 1, wherein: the number of the electric heating tubes (7) is four, the four electric heating tubes (7) are arranged on the outer side of the metal heating groove body (6) at equal intervals, the number of the heat dissipation fins (8) is three, and the three heat dissipation fins (8) are arranged on the outer side of the metal heating groove body (6) at equal intervals.
4. The PCR thermal cycler apparatus of claim 1, wherein: the right side fixedly connected with deflector of driving motor (11), and elevator (13) and deflector sliding connection, the inboard fixedly connected with sealing washer of thermal cycle case (1), and the sealing washer is hugged closely with apron (14).
5. The PCR thermal cycler apparatus of claim 1, wherein: the heat-conducting silica gel gasket (9) is positioned between the sample tank body (10) and the metal heating tank body (6), and the heat-conducting silica gel gasket (9) is tightly attached to the sample tank body (10).
6. The PCR thermal cycler apparatus of claim 1, wherein: the micro pump (3), the electric heating tube (7), the driving motor (11), the heat radiation fan (16) and the temperature sensor (17) are all electrically connected with the controller (18).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022156321.4U CN213475992U (en) | 2020-09-27 | 2020-09-27 | PCR thermal cycling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022156321.4U CN213475992U (en) | 2020-09-27 | 2020-09-27 | PCR thermal cycling device |
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|---|---|
| CN213475992U true CN213475992U (en) | 2021-06-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022156321.4U Expired - Fee Related CN213475992U (en) | 2020-09-27 | 2020-09-27 | PCR thermal cycling device |
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| Country | Link |
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| CN (1) | CN213475992U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117143723A (en) * | 2023-09-05 | 2023-12-01 | 江苏吉诺思美精准医学科技有限公司 | PCR detection device and method |
-
2020
- 2020-09-27 CN CN202022156321.4U patent/CN213475992U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117143723A (en) * | 2023-09-05 | 2023-12-01 | 江苏吉诺思美精准医学科技有限公司 | PCR detection device and method |
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Granted publication date: 20210618 |