CN213447144U - Freezing tube water bath device for cell resuscitation - Google Patents
Freezing tube water bath device for cell resuscitation Download PDFInfo
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- CN213447144U CN213447144U CN202021415833.1U CN202021415833U CN213447144U CN 213447144 U CN213447144 U CN 213447144U CN 202021415833 U CN202021415833 U CN 202021415833U CN 213447144 U CN213447144 U CN 213447144U
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Abstract
The utility model discloses a cryovial water bath device for cell resuscitation, which comprises a body, body inside is provided with heater and temperature control unit, the temperature control unit is including the temperature sensor who is used for measuring the internal water bath temperature and the controller of setting in the body bottom, temperature sensor's detected signal sends into differential mode amplifier circuit and steady voltage filter circuit in proper order and handles, differential mode amplifier circuit adopts LC filter network to carry out noise reduction to temperature sensor's output signal, the interference of external high frequency miscellaneous wave has been eliminated well, then the application differential mode principle of amplification can effectively eliminate the common mode interference in the detected signal output process, improve the signal amplification precision; the voltage stabilizing filter circuit is used for stabilizing the amplitude of the signal, then the signal is subjected to low-pass noise reduction by the LC and then sent to the A/D converter for analog-to-digital conversion, the controller is used for monitoring the water bath temperature value in real time, and the working state of the heater is controlled to keep the water bath temperature close to a set value all the time, so that the normal recovery of cells is ensured.
Description
Technical Field
The utility model relates to a cell biology technical field especially relates to cryovial water bath device for cell resuscitation.
Background
In the field of cell biology, an important mode for preserving cell biological resources is low-temperature freezing, and when the experiment needs, frozen cells are taken out and subjected to water bath recovery. When the cells are subjected to water bath resuscitation, the best operation is to rapidly heat the cell freezing tube body to 37 ℃ so as to reduce the damage to the cells. Need carry out accurate control to the water bath temperature during this period, avoid the temperature too high cell death that leads to the fact, or the temperature is crossed and is made the cell completely resuscitate excessively, and current water bath device temperature regulator leads to the temperature control effect poor owing to there is the inaccurate condition of temperature detection in the cryovial body is heated inequality, is unfavorable for the cell to normally resuscitate.
SUMMERY OF THE UTILITY MODEL
In view of the above situation, in order to overcome the defects of the prior art, the present invention aims to provide a freezing tube water bath device for cell resuscitation.
The technical scheme for solving the problem is as follows: freezing pipe water bath device for cell resuscitation, including the body, body inside is provided with heater and temperature control unit, temperature control unit is including the temperature sensor who is used for measuring this internal water bath temperature and the controller of setting in the body bottom, temperature sensor's detected signal sends into differential mode amplifier circuit and steady voltage filter circuit in proper order and handles, send into after AD converter conversion digital quantity of steady voltage filter circuit's output signal in the controller, the controller is used for controlling the operating condition of heater.
Preferably, the differential mode amplifying circuit includes operational amplifiers AR1 and AR2, a non-inverting input terminal of the operational amplifier AR1 is connected to one ends of capacitors C1 and C2 and an inductor L1, a non-inverting input terminal of the operational amplifier AR2 is connected to one ends of capacitors C3 and L2 and the other end of capacitor C1, and is grounded through a resistor R5, the other ends of capacitors C2 and C28 are grounded, the other ends of inductors L1 and L2 are respectively connected to a first signal output terminal and a second signal output terminal of the temperature sensor, an inverting input terminal of the operational amplifier AR1 is connected to one ends of resistors R1 and R2, the other end of resistor R1 is grounded, the other end of resistor R2 is connected to an output terminal of the operational amplifier AR1, and is connected to an inverting input terminal of the operational amplifier AR 3 and one end of the resistor R3, and the other end of the resistor R3 is connected to an output terminal of the operational amplifier AR.
Preferably, the voltage stabilizing filter circuit comprises a MOS transistor Q1, a drain of the MOS transistor Q1 is connected to one ends of resistors R6 and R7, the other end of the resistor R6 is connected to an output end of the operational amplifier AR2, the other end of the resistor R7 is connected to a gate of the MOS transistor Q1 and a cathode of a voltage stabilizing diode DZ4, an anode of the voltage stabilizing diode DZ4 is grounded, a source of the MOS transistor Q1 is connected to one end of the a/D converter and a capacitor C4 through an inductor L3, and the other end of the capacitor C4 is grounded.
Preferably, the controller is a single chip microcomputer.
Through the technical scheme, the beneficial effects of the utility model are that:
1. the utility model adopts the temperature sensor to measure the temperature of the water bath in the body in real time, the differential mode amplifying circuit adopts the LC filter network to reduce the noise of the output signal of the temperature sensor, the interference of external high-frequency noise is well eliminated, then the differential mode amplifying principle is applied to effectively eliminate the common mode interference in the process of detecting the output signal, and the signal amplifying precision is improved;
2. the single chip microcomputer monitors the water bath temperature value in real time, when the water bath temperature value does not reach a set value, the single chip microcomputer controls the heater to continuously heat the water in the body, and when the water bath temperature value reaches the set value, the single chip microcomputer controls the heater to be powered off and stop heating, so that the water bath temperature is always kept near the set value, and normal recovery of cells is guaranteed.
Drawings
Fig. 1 is a schematic circuit diagram of the temperature control unit of the present invention.
Detailed Description
The foregoing and other technical matters, features and effects of the present invention will be apparent from the following detailed description taken in conjunction with the accompanying fig. 1. The structural contents mentioned in the following embodiments are all referred to the attached drawings of the specification.
Exemplary embodiments of the present invention will be described below with reference to the accompanying drawings.
Freezing pipe water bath device for cell resuscitation, which comprises a body, inside heater and the temperature control unit of being provided with of body, the temperature control unit is including the temperature sensor who is used for measuring this internal water bath temperature and the controller of setting in the body bottom, temperature sensor's detected signal sends into differential mode amplifier circuit and steady voltage filter circuit in proper order and handles, during steady voltage filter circuit's output signal sent into the controller after the AD converter converts the digital quantity into, the controller is the singlechip for the operating condition of control heater.
The model of the temperature sensor is PT100, and the detection probe of the temperature sensor is arranged in the middle of the freezing pipe, so that the effectiveness of water temperature measurement is guaranteed. The detection signal of the temperature sensor is firstly sent to a differential mode amplifying circuit for signal enhancement processing, as shown in fig. 1, the differential mode amplifying circuit comprises operational amplifiers AR1 and AR2, the non-inverting input end of the operational amplifier AR1 is connected with one ends of capacitors C1, C2 and an inductor L1, the non-inverting input end of the operational amplifier AR2 is connected with one ends of the capacitor C3 and the inductor L2 and the other end of the capacitor C1, the other ends of the capacitors C2 and C3 are grounded through a resistor R5, the other ends of the inductors L1 and L2 are respectively connected with a first signal output end and a second signal output end of the temperature sensor, the inverting input end of the operational amplifier AR1 is connected with one ends of the resistors R1 and R2, the other end of the resistor R1 is grounded, the other end of the resistor R2 is connected with the output end of the operational amplifier AR1, the inverting input terminal of the operational amplifier AR2 and one end of the resistor R4 are connected through a resistor R3, and the other end of the resistor R4 is connected to the output terminal of the operational amplifier AR 2.
The working principle of the differential mode amplifying circuit is as follows: the two-wire output signal of the PT100 is sent into an LC filter network formed by inductors L1 and L2 and capacitors C1-C3 for noise reduction, and the interference of external high-frequency noise is well eliminated. Then, differential mode amplification is carried out on the signals subjected to LC filtering by a differential amplifier formed by the operational amplifiers AR1 and AR2, common mode interference in the process of outputting the detection signals can be effectively eliminated by applying the differential mode amplification principle, and the signal amplification precision is improved.
The voltage stabilizing filter circuit comprises a MOS transistor Q1, the drain electrode of the MOS transistor Q1 is connected with one end of a resistor R6 and one end of a resistor R7, the other end of the resistor R6 is connected with the output end of an operational amplifier AR2, the other end of the resistor R7 is connected with the grid electrode of the MOS transistor Q1 and the cathode of a voltage stabilizing diode DZ4, the anode of the voltage stabilizing diode DZ4 is grounded, the source electrode of the MOS transistor Q1 is connected with one end of an A/D converter and a capacitor C4 through an inductor L3, and the other end of the capacitor C4 is grounded.
The output signal of the operational amplifier AR2 is sent to the MOS transistor Q1 for amplification, and the zener diode DZ4 plays a role in stabilizing the reference of the gate voltage of the MOS transistor Q1, thereby ensuring that the output voltage of the source of the MOS transistor Q1 has good stability. The inductor L3 and the capacitor C4 form an LC filter to further reduce the noise of the output signal of the MOS transistor Q1 in a low-pass mode, and finally the output signal is sent into the single chip microcomputer after analog-to-digital conversion.
The utility model discloses when specifically using, this internal water bath temperature of temperature sensor real-time measurement, differential mode amplifier circuit adopt LC filter network to fall the noise treatment to temperature sensor's output signal, have eliminated the interference of external high frequency noise well, then utilize the differential mode to enlarge the principle and can effectively eliminate the common mode interference among the detection signal output process, improve the signal amplification precision. The voltage stabilizing filter circuit stabilizes the amplitude of the output signal of the differential mode amplifying circuit, utilizes LC filtering to further reduce noise, sends the signal into an A/D converter to convert the temperature detection signal value into digital quantity, and finally sends the digital quantity into a singlechip to calculate the temperature. The single chip microcomputer monitors the water bath temperature value in real time, when the water bath temperature value does not reach a set value (37 ℃), the single chip microcomputer controls the heater to continuously heat the water in the body, and when the water bath temperature value reaches the set value, the single chip microcomputer controls the heater to be powered off and stop heating, so that the water bath temperature is always kept near the set value, and normal recovery of cells is guaranteed.
The above description is provided for further details of the present invention with reference to the specific embodiments, which should not be construed as limiting the present invention; to the utility model discloses affiliated and relevant technical field's technical personnel are based on the utility model discloses under the technical scheme thinking prerequisite, the extension of doing and the replacement of operating method, data all should fall within the utility model discloses within the protection scope.
Claims (2)
1. Freezing tube water bath device for cell resuscitation, including the body, body inside is provided with heater and temperature control unit, its characterized in that: the temperature control unit comprises a temperature sensor for measuring the temperature of the water bath in the body and a controller arranged at the bottom of the body, detection signals of the temperature sensor are sequentially sent to a differential-mode amplification circuit and a voltage-stabilizing filter circuit for processing, output signals of the voltage-stabilizing filter circuit are converted into digital quantity through an A/D converter and then sent to the controller, and the controller is used for controlling the working state of the heater;
the differential mode amplifying circuit comprises operational amplifiers AR1 and AR2, wherein the non-inverting input end of an operational amplifier AR1 is connected with one ends of capacitors C1, C2 and an inductor L1, the non-inverting input end of the operational amplifier AR2 is connected with one ends of a capacitor C3, an inductor L2 and the other end of a capacitor C1, and is grounded through a resistor R5, the other ends of capacitors C2 and C3 are grounded, the other ends of inductors L1 and L2 are respectively connected with a first signal output end and a second signal output end of the temperature sensor, the inverting input end of an operational amplifier AR1 is connected with one ends of resistors R1 and R2, the other end of a resistor R1 is grounded, the other end of a resistor R2 is connected with the output end of an operational amplifier AR1, and is connected with the inverting input end of the operational amplifier AR 3 and one end of the resistor R3, and the other end of the resistor R3 is connected;
the voltage-stabilizing filter circuit comprises a MOS transistor Q1, the drain electrode of the MOS transistor Q1 is connected with one ends of a resistor R6 and a resistor R7, the other end of the resistor R6 is connected with the output end of an operational amplifier AR2, the other end of the resistor R7 is connected with the grid electrode of the MOS transistor Q1 and the cathode of a voltage-stabilizing diode DZ4, the anode of the voltage-stabilizing diode DZ4 is grounded, the source electrode of the MOS transistor Q1 is connected with one end of an A/D converter and a capacitor C4 through an inductor L3, and the other end of the capacitor C4 is grounded.
2. The freezing tube water bath device for cell resuscitation as claimed in claim 1, wherein: the controller is a single chip microcomputer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021415833.1U CN213447144U (en) | 2020-07-17 | 2020-07-17 | Freezing tube water bath device for cell resuscitation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021415833.1U CN213447144U (en) | 2020-07-17 | 2020-07-17 | Freezing tube water bath device for cell resuscitation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213447144U true CN213447144U (en) | 2021-06-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021415833.1U Active CN213447144U (en) | 2020-07-17 | 2020-07-17 | Freezing tube water bath device for cell resuscitation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213447144U (en) |
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2020
- 2020-07-17 CN CN202021415833.1U patent/CN213447144U/en active Active
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