CN213266470U - Automatic recovery unit of polypeptide mixed stock solution - Google Patents
Automatic recovery unit of polypeptide mixed stock solution Download PDFInfo
- Publication number
- CN213266470U CN213266470U CN202022056537.3U CN202022056537U CN213266470U CN 213266470 U CN213266470 U CN 213266470U CN 202022056537 U CN202022056537 U CN 202022056537U CN 213266470 U CN213266470 U CN 213266470U
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- liquid
- head
- drawing liquid
- suction head
- suction
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- 238000011084 recovery Methods 0.000 title claims abstract description 23
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 18
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 18
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 18
- 239000011550 stock solution Substances 0.000 title claims description 11
- 239000007788 liquid Substances 0.000 claims abstract description 115
- 238000001802 infusion Methods 0.000 claims abstract description 15
- 239000007779 soft material Substances 0.000 claims abstract description 5
- 238000005086 pumping Methods 0.000 claims description 31
- 230000005611 electricity Effects 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 abstract description 4
- 238000009434 installation Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 22
- 238000001914 filtration Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Peptides Or Proteins (AREA)
Abstract
The utility model discloses an automatic recovery unit of polypeptide mixed stoste relates to the cell culture field, including drawing liquid mechanism, receiving flask and infusion hose, through infusion hose intercommunication installation between the output of drawing liquid mechanism and the receiving flask, the input threaded connection of drawing liquid mechanism has the pipette, and the bottom threaded connection of pipette has the liquid transfer head, moves the liquid head and includes suction nozzle and suction head, the shape of suction nozzle and suction head is the taper shape, the bottom at the suction nozzle is established to the suction head, the suction head is made by soft materials. The utility model discloses a set up the suction nozzle and the suction head of toper form, when solution inhales in the pipette, the suction nozzle and the inside aperture top-down of suction head of toper form dwindle gradually, have restricted the downflow rate of solution, when stopping the drawing liquid, the effectual solution that has prevented in the pipette or move the liquid head flows out, ensures the stability of solution recovery, and soft suction head can be better absorbs a small amount of and dissolves.
Description
Technical Field
The utility model relates to a cell culture field, in particular to automatic recovery unit of polypeptide mixed stock solution.
Background
Present cell culture solution is retrieved most and is used the pipette or directly topples over the mode and retrieve, directly topple over and empty unclean, can remain some liquid in the cell culture bottle, drip liquid in the blake bottle outside easily, lead to the emergence of cell pollution like this, use the pipette to retrieve, more solution need carry out the recovery in-process, need constantly absorb the operation of discharging, waste time and energy, current imbibition device is behind absorbing solution, the suction head part is cylindricly, the solution part of absorption drips easily outside the receiving flask, cause the pollution of cell or operational environment easily, and when collecting to polypeptide mixing stoste, the fragment and the cell gathering piece that contain a large amount of cells in the polypeptide mixing stoste, need carry out filtration operation on next step after the recovery, it is comparatively loaded down with trivial details to retrieve the flow.
Therefore, it is necessary to develop an automatic recovery device for polypeptide mixed stock solution to solve the above problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an automatic recovery unit of polypeptide mixed stoste to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides an automatic recovery unit of mixed stoste of polypeptide, includes drawing liquid mechanism, receiving flask and infusion hose, through infusion hose intercommunication installation between the output of drawing liquid mechanism and the receiving flask, the input threaded connection of drawing liquid mechanism has the pipette, and the bottom threaded connection of pipette has the liquid transfer head, and the liquid transfer head includes suction nozzle and suction head, the shape of suction nozzle and suction head is conical, the bottom at the suction nozzle is established to the suction head, the suction head is made by soft materials, the receiving flask includes bottle lid, cartridge filter and liquid collecting bottle, bottle lid, cartridge filter and liquid collecting bottle are threaded installation in order, the top external diameter value of cartridge filter equals the top external diameter value of liquid collecting bottle.
Optionally, the liquid pumping mechanism includes a liquid pumping pump and a liquid pumping pipe, and a liquid outlet is formed below the side surface of the liquid pumping pipe.
Optionally, the anti-skidding cover has been cup jointed in the outside of drawing liquid pipe, be equipped with the drawing liquid switch on the drawing liquid pipe, the drawing liquid switch runs through the anti-skidding cover, the drawing liquid switch is connected with drawing liquid pump electricity.
The upper part of the pipette is held in the vertical direction, and the liquid pumping switch is arranged on the holding part, so that the device is convenient to operate and control.
Optionally, a liquid inlet interface and a vacuum interface are arranged on the bottle cap, one end of the infusion hose is communicated with the liquid inlet interface, and the other end of the infusion hose is communicated with the liquid outlet interface.
Optionally, the inside vertical parallel of cartridge filter is equipped with the multilayer filter screen, the collection liquid bottle is transparent.
The transparent liquid collecting bottle is convenient for observing the recovery amount in the bottle.
The utility model discloses a technological effect and advantage:
1. the utility model discloses a set up drawing liquid mechanism, receiving flask and infusion hose and cooperation use, utilize the drawing liquid pump in the drawing liquid mechanism to carry out electronic drawing liquid, drawing liquid speed is fast, and drawing liquid mechanism extracts liquid and directly retrieves to the receiving flask in, can realize once only continuously retrieving, has promoted recovery efficiency, subtracts and has saved the hand labor power.
2. The utility model discloses a set up the suction nozzle and the suction head of toper form, when solution inhales in the pipette, the suction nozzle and the inside aperture top-down of suction head of toper form dwindle gradually, have restricted the downflow rate of solution, when stopping the drawing liquid, the effectual solution that has prevented in the pipette or move the liquid head flows out, ensures the stability of solution recovery, a small amount of solution of absorption that soft suction head can be better.
3. The utility model discloses the receiving flask comprises bottle lid, cartridge filter and liquid collecting bottle threaded connection in proper order, and when solution was collected, the filter screen in the cartridge filter can carry out filtration treatment to solution, and the cartridge filter of threaded mounting is convenient for tear open and washes the washing to cartridge filter and liquid collecting bottle all can with bottle lid threaded mounting, and the cartridge filter can regard as the apolegamy device to use, and the limitation is little.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural view of the liquid pumping mechanism of the present invention.
Fig. 3 is a schematic view of the structure of the liquid-transferring head of the present invention.
Fig. 4 is the schematic view of the structure of the collection bottle of the present invention.
In the figure: 1. a liquid pumping mechanism; 101. a liquid pump; 102. a liquid pumping pipe; 2. a collection bottle; 201. a bottle cap; 202. a filter cartridge; 203. a liquid collecting bottle; 3. a flexible infusion tube; 4. a pipette; 5. a pipetting head; 501. a suction nozzle; 502. a suction head; 6. a liquid outlet interface; 7. an anti-slip sleeve; 8. a liquid extraction switch; 9. a liquid inlet interface; 10. a vacuum interface; 11. and (4) a filter screen.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
In the present invention, unless otherwise expressly stated or limited, the terms "disposed," "mounted," "connected," and "fixed" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; may be a mechanical connection; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
The utility model provides an automatic recovery device of polypeptide mixed stock solution as shown in figures 1-4, which comprises a liquid pumping mechanism 1, a collection bottle 2 and a transfusion hose 3, wherein the output end of the liquid pumping mechanism 1 is communicated with the collection bottle 2 through the transfusion hose 3, the liquid pumping mechanism 1 comprises a liquid pumping pump 101 and a liquid pumping pipe 102, the liquid pumping pump 101 and the liquid pumping pipe 102 are the existing vertical liquid pumping devices, a liquid outlet port 6 is arranged below the side surface of the liquid pumping pipe 102, an anti-skid sleeve 7 is sleeved outside the liquid pumping pipe 102, a liquid pumping switch 8 is arranged on the liquid pumping pipe 102, the liquid pumping switch 8 penetrates through the anti-skid sleeve 7, the liquid pumping switch 8 is electrically connected with the liquid pumping pump 101, the anti-skid sleeve 7 is held by hand, electric liquid pumping can be performed by pressing the liquid pumping switch 8, the liquid pumping speed is high, the input end of the liquid pumping mechanism 1 is in threaded connection with a pipette 4, the bottom end of the pipette 4, the pipetting head 5 comprises a suction nozzle 501 and a suction head 502, the suction nozzle 501 and the suction head 502 are both in the shape of a cone, the inner pore diameters of the cone-shaped suction nozzle 501 and the cone-shaped suction head 502 are gradually reduced from top to bottom, the down flow rate of the solution is limited, when the pipetting is stopped, the solution in the pipette 4 or the pipetting head 5 is effectively prevented from flowing out, the stability of the solution recovery is ensured, the suction head 502 is arranged at the bottom end of the suction nozzle 501, the suction head 502 is made of soft materials, the collection bottle 2 comprises a bottle cap 201, a filter cylinder 202 and a liquid collection bottle 203, the bottle cap 201, the filter cylinder 202 and the liquid collection bottle 203 are sequentially installed in a threaded manner, the outer diameter value at the top of the filter cylinder 202 is equal to the outer diameter value at the top of the liquid collection bottle 203, the filter cylinder 202 and the liquid collection bottle 203 can be installed with the bottle cap 201 in a, the sealing performance of the collecting bottle 2 is guaranteed, one end of the infusion hose 3 is communicated with the liquid inlet interface 9, the other end of the infusion hose 3 is communicated with the liquid outlet interface 6, the multilayer filter screen 11 is vertically arranged in parallel in the filter cartridge 202, and the collecting bottle 203 is transparent.
The utility model discloses the theory of operation:
when in use, the bottle cap 201, the filter cartridge 202 and the liquid collection bottle 203 are assembled, the vacuum pump is connected with the vacuum interface 10 on the bottle cap 201, the gas in the collection bottle 2 is evacuated to prevent the polypeptide stock solution from being affected by contact with air, the liquid transfer head 5 is placed in the polypeptide stock solution, the antiskid sleeve 7 is held by hand, the liquid transfer pump 101 is started by pressing the liquid transfer switch 8, the liquid transfer pump 101 is used as a power source for liquid transfer, the liquid transfer rate is high, the solution enters the liquid transfer head 4 from the liquid transfer head 5 and is input into the collection bottle 2 through the infusion hose 3, the extracted liquid is directly recovered into the collection bottle 2, one-time continuous recovery can be realized, the recovery efficiency is improved, the labor force is reduced, the suction head 502 is made of soft material, when the solution is about to be completely sucked, the soft suction head 502 is obliquely pressed to slightly deform, the solution is convenient for better sucking, and the suction nozzle 501, the suction nozzle 502 internal diameter value is less than the internal diameter value of suction nozzle 501, the internal aperture top-down of suction nozzle 501 and suction nozzle 502 of toper form dwindles gradually, the downflow rate of solution has been restricted, when stopping drawing liquid, the effectual solution that has prevented in pipette 4 or the pipette head 5 flows out, ensure the stability of solution recovery, polypeptide solution is retrieved to collecting flask 2 in, flow into collecting flask 203 and collect after the filtration of multilayer filter screen 11, and cartridge filter 202 and collecting flask 203 all can with bottle lid 201 threaded mounting, cartridge filter 202 can regard as the apolegamy device to use, the limitation is little, this device connecting portion is detachable construction, be convenient for wash or deposit it.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (5)
1. The utility model provides an automatic recovery unit of mixed stoste of polypeptide, includes drawing liquid mechanism (1), receiving flask (2) and infusion hose (3), install its characterized in that through infusion hose (3) intercommunication between the output of drawing liquid mechanism (1) and receiving flask (2): the utility model discloses a liquid collection device, including drawing liquid mechanism (1), the input end threaded connection of drawing liquid mechanism (1) has pipette (4), and the bottom threaded connection of pipette (4) has liquid transfer head (5), and liquid transfer head (5) include suction nozzle (501) and suction head (502), the shape of suction nozzle (501) and suction head (502) is conical, the bottom at suction nozzle (501) is established in suction head (502), suction head (502) are made by soft material, receiving flask (2) are including bottle lid (201), cartridge filter (202) and liquid collecting flask (203), bottle lid (201), cartridge filter (202) and liquid collecting flask (203) threaded mounting in order, the top external diameter value of cartridge filter (202) equals the top external diameter value of liquid collecting flask (203).
2. The automatic recovery device of a polypeptide mixed stock solution according to claim 1, wherein: the liquid pumping mechanism (1) comprises a liquid pumping pump (101) and a liquid pumping pipe (102), and a liquid outlet interface (6) is formed below the side face of the liquid pumping pipe (102).
3. The automatic recovery device of a polypeptide mixed stock solution according to claim 2, wherein: the anti-skidding cover (7) has been cup jointed in the outside of drawing liquid pipe (102), be equipped with drawing liquid switch (8) on drawing liquid pipe (102), drawing liquid switch (8) run through anti-skidding cover (7), drawing liquid switch (8) are connected with drawing liquid pump (101) electricity.
4. The automatic recovery device of a polypeptide mixed stock solution according to claim 1 or 2, wherein: the liquid inlet interface (9) and the vacuum interface (10) are arranged on the bottle cap (201), one end of the infusion hose (3) is communicated with the liquid inlet interface (9) and is installed, and the other end of the infusion hose (3) is communicated with the liquid outlet interface (6) and is installed.
5. The automatic recovery device of a polypeptide mixed stock solution according to claim 1, wherein: the inside of cartridge filter (202) is vertical parallel to be equipped with multilayer filter screen (11), liquid collecting bottle (203) are transparent.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202022056537.3U CN213266470U (en) | 2020-09-18 | 2020-09-18 | Automatic recovery unit of polypeptide mixed stock solution |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202022056537.3U CN213266470U (en) | 2020-09-18 | 2020-09-18 | Automatic recovery unit of polypeptide mixed stock solution |
Publications (1)
Publication Number | Publication Date |
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CN213266470U true CN213266470U (en) | 2021-05-25 |
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Family Applications (1)
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CN202022056537.3U Active CN213266470U (en) | 2020-09-18 | 2020-09-18 | Automatic recovery unit of polypeptide mixed stock solution |
Country Status (1)
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CN (1) | CN213266470U (en) |
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2020
- 2020-09-18 CN CN202022056537.3U patent/CN213266470U/en active Active
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