CN213239599U - Flow cytometer detects and uses sampling device - Google Patents

Flow cytometer detects and uses sampling device Download PDF

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Publication number
CN213239599U
CN213239599U CN202022413574.5U CN202022413574U CN213239599U CN 213239599 U CN213239599 U CN 213239599U CN 202022413574 U CN202022413574 U CN 202022413574U CN 213239599 U CN213239599 U CN 213239599U
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CN
China
Prior art keywords
sampling
flow cytometer
test tube
internal gear
rack
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Expired - Fee Related
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CN202022413574.5U
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Chinese (zh)
Inventor
刘艳红
余孝其
王娜
李坤
张骥
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Sichuan University
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Sichuan University
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Publication date
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Priority to CN202022413574.5U priority Critical patent/CN213239599U/en
Application granted granted Critical
Publication of CN213239599U publication Critical patent/CN213239599U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses a flow cytometer detects and uses sampling device relates to cell sample field, comprises a fixed bench, the motor is installed at the top of fixed station, the output of motor is taken out and is connected with the axis of rotation through the shaft coupling, the top of axis of rotation is connected with the driving gear, the driving gear meshing has the internal gear, the top of internal gear is provided with the mount table, the top of mount table is provided with the rack, the spacing hole of multiunit has been seted up at the top of rack. The utility model discloses a place the test tube on the rack, start electric putter, the installation piece moves down, the sampling tube stretches into the test tube, start the delivery pump, detect cell from conveyer pipe suction detection device in the test tube, when needs extract different types of cell, starter motor, under driving gear and internal gear cooperation, the test tube on the rack is rotatory thereupon, when moving the sampling tube below, the repeated sample detection work that takes a sample, the sampling efficiency is improved, the demand of cell experiment has been satisfied.

Description

Flow cytometer detects and uses sampling device
Technical Field
The utility model relates to a cell sample field specifically is a flow cytometer detects uses sampling device.
Background
Flow cytometric sampling devices are devices for automated analysis and sorting of cells that rapidly measure, store and display a range of important biophysical, biochemical characteristic parameters of dispersed cells suspended in a liquid, and can sort out specific cell subsets according to preselected parameter ranges.
Current flow cytometer when taking a sample to the cell stoste, need absorb the cell stoste with the sampling tube, the cell stoste is generally placed in the test tube, and when treating the cell stoste kind of sample too much, it is not very convenient to change the test tube manually, has caused certain influence to the speed of cell detection sample, has reduced sampling efficiency, can not satisfy the demand of experiment.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a: in order to solve the problem of low sampling rate of a general sampling device, a sampling device for flow cytometry detection is provided.
In order to achieve the above object, the utility model provides a following technical scheme: a sampling device for flow cytometer detection comprises a fixed table, wherein a motor is installed at the top of the fixed table, an output drawer of the motor is connected with a rotating shaft through a coupler, the top end of the rotating shaft is connected with a driving gear, the driving gear is meshed with an internal gear, the top of the internal gear is provided with an installation table, the top of the installation table is provided with a placement frame, the top of the placement frame is provided with a plurality of groups of limiting holes, the inner wall of each limiting hole is movably provided with a test tube, the top of the fixed table is provided with a detection device, one side of the detection device is provided with a fixed block, the bottom of the fixed block is provided with an electric push rod, the movable end of the electric push rod is connected with an installation block, the bottom of the installation block is provided with a sampling tube, and a delivery pump is arranged in the middle of the delivery pipe.
Preferably, a bearing is arranged at the top of the fixed table, and a movable shaft is arranged on the inner wall of the bearing.
Preferably, the top of mount table is seted up multiunit spacing groove, and the shape size of spacing groove all matches each other with the shape size of test tube.
Preferably, the one end that detection device was kept away from to the conveyer pipe is connected with the sampling tube, and the hookup location of conveyer pipe and sampling tube is provided with the sealing washer.
Preferably, the internal gear is connected with the mounting table through a bolt, and the internal gear and the mounting table are arranged concentrically.
Preferably, the inner wall of the limiting groove is provided with a soft cushion, and the soft cushion is a rubber soft cushion.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model discloses a place the test tube on the rack, start electric putter, the installation piece moves down, the sampling tube stretches into the test tube, start the delivery pump, detect cell in the test tube from conveyer pipe suction detection device, when needing to extract different types of cell stoste, the starter motor, the output shaft of motor drives the axis of rotation and rotates, under the cooperation of driving gear and internal gear, the mount table begins to rotate, the test tube on the rack rotates thereupon, when moving to the sampling tube below, the repeated sampling detection work that takes a sample, do not need to change the test tube manually, the speed of cell detection sample has been increased, the sampling efficiency is improved, the demand of cell experiment has been satisfied;
2. the utility model discloses an in putting the spacing hole on the rack with the test tube, the bottom and the spacing groove of test tube cooperate, and the test tube can be fixed on the rack, can not take place to shake the skew when taking a sample, improves the stability on the test tube rack, can not take place to empty when the mount table is rotatory, increases cell stock solution sample detection's security.
Drawings
FIG. 1 is a front cross-sectional view of the present invention;
fig. 2 is a structural plan view of the rack of the present invention;
fig. 3 is a schematic view illustrating the structural connection between the driving gear and the internal gear according to the present invention.
In the figure: 1. a fixed table; 2. a motor; 3. a rotating shaft; 4. a driving gear; 5. an internal gear; 6. a movable shaft; 7. a bearing; 8. an installation table; 9. placing a rack; 10. a limiting hole; 11. a limiting groove; 12. a test tube; 13. a detection device; 14. a fixed block; 15. a delivery pump; 16. a delivery pipe; 17. an electric push rod; 18. mounting blocks; 19. and a sampling tube.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "connected" and "disposed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art. The following describes an embodiment of the present invention according to its overall structure.
Referring to fig. 1-3, a sampling device for flow cytometer detection comprises a fixed table 1, a motor 2 is installed on the top of the fixed table 1, an output shaft of the motor 2 is connected with a rotating shaft 3 through a coupler, the top end of the rotating shaft 3 is connected with a driving gear 4, the driving gear 4 is meshed with an internal gear 5, the top of the internal gear 5 is provided with an installation table 8, the top of the installation table 8 is provided with a placement frame 9, the top of the placement frame 9 is provided with a plurality of sets of limiting holes 10, the inner walls of the limiting holes 10 are movably provided with test tubes 12, the test tubes 12 are placed in the limiting holes 10 on the placement frame 9, the bottoms of the test tubes 12 are matched with limiting grooves 11, the test tubes 12 can be fixed on the placement frame 9, no vibration and deviation occur during sampling, the stability on the placement frame 9 of the test tubes 12 is improved, no, the top of the fixed platform 1 is provided with a detection device 13, one side of the detection device 13 is provided with a fixed block 14, the bottom of the fixed block 14 is provided with an electric push rod 17, the movable end of the electric push rod 17 is connected with an installation block 18, the bottom of the installation block 18 is provided with a sampling tube 19, one side of the detection device 13 is connected with a conveying tube 16 which penetrates through the fixed block 14 and extends to the inside of the installation block 18, the middle of the conveying tube 16 is provided with a conveying pump 15, the test tube 12 is placed on a placing frame 9, the electric push rod 17 is started, the installation block 18 moves downwards, the sampling tube 19 extends into the test tube 12, the conveying pump 15 is started, cells in the test tube 12 are pumped into the detection device 13 from the conveying tube 16 for detection, when different cell stock solutions need to be pumped, the motor 2 is started, the output shaft of the motor 2 drives the rotating shaft 3, test tube 12 on rack 9 is rotatory thereupon, when moving to the 19 below of sampling tube, and the repeated sample detection work that takes a sample need not manually change test tube 12, has increased the speed of cell detection sample, has improved sample efficiency, has satisfied the demand of cell experiment.
Please refer to fig. 1 and fig. 3, a bearing 7 is disposed at the top of the fixed platform 1, a movable shaft 6 is disposed on the inner wall of the bearing 7, and the movable shaft 6 and the bearing 7 are disposed to effectively assist the rotation and support of the mounting platform 8.
Please refer to fig. 1, the top of the mounting platform 8 is provided with a plurality of sets of limiting grooves 11, and the shape and size of the limiting grooves 11 are matched with the shape and size of the test tube 12, so that the stability of the test tube 12 is effectively improved by the limiting grooves 11.
Please refer to fig. 1, one end of the delivery tube 16 far away from the detection device 13 is connected to the sampling tube 19, and a sealing ring is disposed at the connection position of the delivery tube 16 and the sampling tube 19, so as to effectively reduce the probability of contamination during cell sampling.
Please refer to fig. 1 and fig. 3, the internal gear 5 is connected to the mounting platform 8 by bolts, and the internal gear 5 is concentrically arranged with the mounting platform 8, so that the rotation effect of the mounting platform 8 is effectively increased by concentrically arranging the internal gear 5 with the mounting platform 8.
Please refer to fig. 1, the inner walls of the limiting grooves 11 are all provided with soft pads, and the soft pads are rubber soft pads, rubber is a high-elasticity polymer material with reversible deformation, and is rich in elasticity at room temperature, can generate large deformation under the action of small external force, and can recover the original shape after the external force is removed, and the safety of the test tube 12 during placement is effectively improved through the arranged rubber soft pads.
The working principle is as follows: put into test tube 12 in the spacing hole 10 on the rack 9, the bottom of test tube 12 cooperatees with spacing groove 11, test tube 12 can be fixed on rack 9, can not take place vibrations skew when taking a sample, start electric putter 17, installation piece 18 moves down, sampling tube 19 stretches into test tube 12, start delivery pump 15, detect cell from 16 suction detection device 13 of conveyer pipe in the test tube 12, when needs draw different types of cell stoste, starter motor 2, motor 2's output shaft drives axis of rotation 3 and rotates, under driving gear 4 and internal gear 5 cooperation, mount table 8 begins to rotate, bearing 7 and loose axle 6 have played support and supplementary rotatory effect to mount table 8, test tube 12 on the rack 9 is rotatory along with mount table 8, when moving under sampling tube 19, the repeated sample detection work that takes place.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (6)

1. A flow cytometer detects and uses sampling device, includes fixed station (1), its characterized in that: the testing device is characterized in that a motor (2) is installed at the top of a fixed table (1), an output pump of the motor (2) is connected with a rotating shaft (3) through a coupler, the top end of the rotating shaft (3) is connected with a driving gear (4), the driving gear (4) is meshed with an internal gear (5), the top of the internal gear (5) is provided with an installation table (8), the top of the installation table (8) is provided with a placing frame (9), the top of the placing frame (9) is provided with a plurality of groups of limiting holes (10), the inner wall of each limiting hole (10) is movably provided with a test tube (12), the top of the fixed table (1) is provided with a detection device (13), one side of the detection device (13) is provided with a fixed block (14), the bottom of the fixed block (14) is provided with an electric push rod (, the bottom of installation piece (18) is provided with sampling tube (19), one side of detection device (13) is connected with and runs through fixed block (14) and extend to installation piece (18) inside conveyer pipe (16), and the centre of conveyer pipe (16) is installed delivery pump (15).
2. A flow cytometer detection sampling device as described in claim 1, wherein: the top of fixed station (1) is provided with bearing (7), the inner wall of bearing (7) is provided with loose axle (6).
3. A flow cytometer detection sampling device as described in claim 1, wherein: a plurality of groups of limiting grooves (11) are formed in the top of the mounting table (8), and the shapes and the sizes of the limiting grooves (11) are matched with those of the test tubes (12).
4. A flow cytometer detection sampling device as described in claim 1, wherein: one end of the conveying pipe (16) far away from the detection device (13) is connected with the sampling pipe (19), and a sealing ring is arranged at the connecting position of the conveying pipe (16) and the sampling pipe (19).
5. A flow cytometer detection sampling device as described in claim 1, wherein: the internal gear (5) is connected with the mounting table (8) through bolts, and the internal gear (5) and the mounting table (8) are arranged concentrically.
6. A flow cytometer detection sampling device as described in claim 3, wherein: the inner wall of the limiting groove (11) is provided with soft pads, and the soft pads are rubber soft pads.
CN202022413574.5U 2020-10-27 2020-10-27 Flow cytometer detects and uses sampling device Expired - Fee Related CN213239599U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022413574.5U CN213239599U (en) 2020-10-27 2020-10-27 Flow cytometer detects and uses sampling device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022413574.5U CN213239599U (en) 2020-10-27 2020-10-27 Flow cytometer detects and uses sampling device

Publications (1)

Publication Number Publication Date
CN213239599U true CN213239599U (en) 2021-05-18

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Family Applications (1)

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CN202022413574.5U Expired - Fee Related CN213239599U (en) 2020-10-27 2020-10-27 Flow cytometer detects and uses sampling device

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117244706A (en) * 2023-09-18 2023-12-19 浙江基预科技有限公司 Single-cell controllable detection device and detection method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117244706A (en) * 2023-09-18 2023-12-19 浙江基预科技有限公司 Single-cell controllable detection device and detection method thereof

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Granted publication date: 20210518