CN213091549U

CN213091549U - Microfluidic carbohydrate metabolism analysis and detection device

Info

Publication number: CN213091549U
Application number: CN202021800223.3U
Authority: CN
Inventors: 杈逛豢; 边仿
Original assignee: Tianjin Haixing Technology Co ltd
Current assignee: Tianjin Haixing Technology Co ltd
Priority date: 2020-08-25
Filing date: 2020-08-25
Publication date: 2021-04-30
Anticipated expiration: 2030-08-25

Abstract

The utility model provides a micro-fluidic saccharide metabolic analysis detection device, include: the device comprises a micro-fluidic sensor unit, a micro-injection pump, a waste liquid pool, a signal circuit, an excitation light source, a light source modulation circuit and a controller; a liquid inlet channel of the microfluidic sensor unit is communicated with the micro-injection pump, and a liquid outlet channel is communicated with the waste liquid pool; the signal circuit applies bias voltage to the microfluidic sensor unit, collects detection signals from the microfluidic sensor unit, is connected with the light source modulation circuit and sends modulation control signals; the excitation light source consists of a plurality of fixed-focus adjustable infrared laser diode modules, is arranged towards the microfluidic sensor unit, is connected with the light source modulation circuit, and emits excitation light for generating photocurrent to the microfluidic sensor unit; the light source modulation circuit modulates the exciting light according to the modulation control signal; the controller is in communication connection with the signal circuit and is used for detecting, controlling, recording and processing the acquired data. The utility model discloses can realize carrying out quantitative analysis to carbohydrate metabolism developments and detect.

Description

Microfluidic carbohydrate metabolism analysis and detection device

Technical Field

The application relates to the field of biosensors, in particular to a microfluidic carbohydrate metabolism analysis and detection device.

Background

The taste sense is a sense produced by food in the oral cavity to stimulate the chemical sensing system of taste organ, and includes four basic taste senses of sweet, sour, bitter and salty. The various tastes that people usually taste are the result of the mixture of the four tastes. Taste receptors are taste buds on the tongue, which are oval and mainly composed of taste cells and supporting cells, wherein microvilli at the top of the taste cells extend towards the taste pores and contact with saliva, and nerve fibers are innervated at the cell base. The chemicals in the food dissolve in the saliva and contact the taste receptor cells through the taste pores, where they interact with taste receptors or ion channels. These interactions trigger intracellular signaling cascades that induce cellular action potentials, which are ultimately transmitted to the brain via nerve fibers, forming the sense of taste. Taste cells have a number of taste-sensing molecules on their surface, and different substances can bind to different taste-sensing molecules to present different tastes. The taste of human is stimulated by food to feel the taste only for 1.5-4.0ms, which is faster than the visual sense for 13-45ms, the auditory sense for 1.27-21.5ms and the tactile sense for 2.4-8.9 ms. Conventionally, taste analysis is mainly determined by an artificial taste analysis method, which has a high subjectivity and is difficult to accurately analyze and evaluate taste brought by food. Therefore, the taste sensor can realize on-site, rapid and real-time detection, and has important significance for the food field. Particularly, dynamic detection under the flow state caused by food consumption is more necessary.

Sugar is an important flavoring agent, and is divided into high-calorie sweeteners and low-calorie sweeteners according to the caloric content. The high calorie sweetener comprises sucrose, honey and other natural sweeteners, and has high calorie, and is easy to cause obesity and even diabetes after long-term over-consumption. Low calorie sweetener refers to a material having sweetness, low caloric power and low nutritional value, which is commonly used for controlling blood sugar elevation, preventing obesity, controlling body weight and preventing cardiovascular disease, and is also used as a sugar substitute for diabetics. Lactic acid bacteria in the oral cavity of humans and other mammals ferment with trace amounts of sugars in food to produce organic acids, including lactic acid, acetic acid (ethanol), and the like, which impart specific flavors to various sugars. The metabolic characteristics of the lactic acid bacteria on different saccharides are accurately analyzed through the biosensor, and the method has great significance for preparing the sweetening agent with low calorie and the taste close to sucrose in the field of food.

SUMMERY OF THE UTILITY MODEL

The utility model aims at the shortcoming that prior art exists, provide a micro-fluidic saccharide metabolic analysis detection device, solve the conventional taste sense analysis that exists among the prior art and detect that the subjectivity is strong, the static accuracy subalternation problem that brings of detection thing.

The utility model provides a micro-fluidic saccharide metabolic analysis detection device, include: the device comprises a micro-fluidic sensor unit, a micro-injection pump, a waste liquid pool, a signal circuit, an excitation light source, a light source modulation circuit and a controller; a liquid inlet channel of the microfluidic sensor unit is communicated with the micro-injection pump, and a liquid outlet channel of the microfluidic sensor unit is communicated with the waste liquid pool; the signal circuit is connected with the micro-fluidic sensor unit, applies bias voltage to the micro-fluidic sensor unit and collects detection signals from the micro-fluidic sensor unit, and is connected with the light source modulation circuit and sends modulation control signals; the excitation light source consists of a plurality of fixed-focus adjustable infrared laser diode modules, is arranged towards the microfluidic sensor unit, is connected with the light source modulation circuit, and emits excitation light for generating photocurrent to the microfluidic sensor unit; the light source modulation circuit is connected with the excitation light source and modulates the excitation light source to emit excitation light according to the modulation control signal; the controller is in communication connection with the signal circuit and is used for detecting, controlling, recording and processing the acquired data.

Preferably, the microfluidic sensor unit comprises a substrate, a light-addressable potential sensor, a microporous membrane, a liquid inlet pipe, a liquid outlet pipe and a working electrode; the conducting layer of the light addressing potential sensor is connected with the working electrode, and the light addressing potential sensor and the working electrode are arranged on the substrate; the microporous membrane is arranged on the optical addressing potential sensor and is provided with a spinning vertical micro flow groove; the liquid inlet pipe and the liquid outlet pipe are arranged at two ends of the micro flow groove and are inserted into the micro-pore membrane.

Preferably, the liquid inlet pipe and the liquid outlet pipe are composed of a metal pipe and a polyethylene microporous pipe, the metal pipe is inserted into the microporous membrane, and the polyethylene microporous pipe is sleeved on the metal pipe.

Preferably, the optical addressing potential sensor is composed of an aluminum layer, a silicon wafer substrate, an insulator layer and a protective layer in sequence from the back side to the front side; the aluminum layer is connected with the working electrode, the protective layer is positioned below the microporous membrane, and the protective layer exposed out of the microfluidic groove is arranged towards the excitation light source.

Preferably, the signal circuit comprises a microcontroller and a LAPS detection circuit; the LAPS detection circuit comprises an I/V conversion circuit, a zero setting and amplifying circuit, a low-pass filter circuit, an impedance circuit and a clock circuit; converting a photocurrent signal of the optical addressing potential sensor into a voltage signal through an I/V conversion circuit; the voltage signal is subjected to error removal and in-phase amplification through a zero setting and amplifying circuit, low-frequency noise is filtered through a low-pass filter circuit, and the low-frequency noise is sent to the microcontroller; the impedance circuit provides a high-frequency alternating current excitation source for the light source modulation circuit to carry out impedance detection and drive the excitation light source; the clock circuit provides a clock signal to the impedance circuit through microprocessor control.

Preferably, a bubble eliminating unit is arranged on the liquid inlet channel and is communicated with the vacuum pump. The bubble eliminating unit comprises an upper substrate, a lower substrate, a negative pressure cavity, a fluid cavity, a vacuum pump connecting port, a micro-flow inlet, a micro-flow outlet, a vacuum pump connecting pipe and a fluid conduit; the negative pressure cavity is communicated with the fluid cavity, the negative pressure cavity and the fluid cavity are positioned between the upper substrate and the lower substrate, the negative pressure cavity is adjacent to the upper substrate, and the fluid cavity is adjacent to the lower substrate; the vacuum pump connecting port is positioned on the upper substrate and is communicated with the negative pressure cavity; the micro-flow inlet and the micro-flow outlet are positioned on the lower substrate and communicated with the fluid cavity.

Compared with the prior art, the utility model discloses the beneficial effect of embodiment is: the utility model realizes dynamic quantitative analysis and detection of carbohydrate lactobacillus metabolism by the micro-fluidic sensor unit obtained by organically combining the optical addressing potential sensor and the micro-fluidic technology; the bubble eliminating unit is arranged on the liquid inlet channel and communicated with the vacuum pump, so that bubbles in the detection solution are effectively eliminated, and the detection precision is improved; based on the utility model discloses an analysis and detection device carries out sugar metabolism and detects, and the lactic acid bacteria metabolic characteristic of different sugars is mastered to the accuracy to carry out sugar taste simulation on this basis and detect, the accurate acidity scope that obtains the compound sweetener that metabolic taste and cane sugar are close.

Drawings

The above features and advantages of the present invention will become more apparent and readily appreciated from the following description of the exemplary embodiments thereof taken in conjunction with the accompanying drawings.

Fig. 1 is a schematic structural diagram of a microfluidic carbohydrate metabolism analysis and detection apparatus according to an embodiment of the present invention.

Fig. 2 is a schematic structural diagram of a microfluidic sensor unit according to an embodiment of the present invention.

Fig. 3 is a schematic structural diagram of an optical addressable potentiometric sensor according to an embodiment of the present invention.

Fig. 4 is a circuit diagram of a signal circuit according to an embodiment of the present invention.

Fig. 5 is a schematic diagram of the mechanism of a microfluidic carbohydrate metabolism analysis and detection device according to another embodiment of the present invention.

Fig. 6 is a schematic structural diagram of a bubble elimination unit according to an embodiment of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the attached drawings so that those skilled in the art can accurately understand the present invention.

Example 1

As shown in fig. 1, the microfluidic carbohydrate metabolism analysis and detection apparatus 100 provided in this embodiment includes: controller 110, signal circuit 120, excitation light source 130, light source modulation circuit 140, microfluidic sensor unit 150, micro-syringe pump 160, and waste reservoir 170. The liquid inlet channel of the micro-fluidic sensor unit 150 is connected with the micro-injection pump 160, the liquid outlet channel of the micro-fluidic sensor unit 150 is connected with the waste liquid pool 170, and the micro-fluidic sensor unit 150 is connected with the signal circuit 120, so that bias voltage and signal acquisition are realized on the micro-fluidic sensor unit 150. The excitation light source 130 is disposed above the microfluidic sensor unit 150 and connected to the light source modulation circuit 140, so that the microfluidic sensor unit 150 is excited by light to generate a photo-generated current. The light source modulation circuit 140 is connected to the signal circuit 120 and controlled by the signal circuit 120. The signal circuit 120 is connected to the controller 110 via a serial port to perform related control, data transmission and processing functions.

The micro syringe pump 160 may control the injection amount of the test solution through an asynchronous receive/transmit protocol (UART). The excitation light source 130 is composed of a plurality of fixed-focus tunable infrared laser diode modules for generating electron-hole pairs.

[ microfluidic sensor Unit ]

As shown in fig. 2, the microfluidic sensor unit 150 of the present embodiment includes a substrate 151, an optical addressing potential sensor 152, a microporous membrane 153, a liquid inlet pipe 154, a liquid outlet pipe 155, and a working electrode 156. The substrate 151 is a 0.5-1mm PMMA plate. The back surface of the photo-addressable potential sensor 152 has a conductive layer connected to the working electrode 156, and the back surface of the photo-addressable potential sensor 152 and the working electrode 156 are fixed on the substrate 151 by a conductive paste. The microporous membrane 153 is fixed on the front side of the optical addressing potential sensor 152 by an adhesive with stable biological characteristics, the microporous membrane 153 is provided with a spinning sag-shaped microflow groove, and the liquid inlet pipe 154 and the liquid outlet pipe 155 are arranged at two ends of the microflow groove and are inserted into and fixed with the microporous membrane 153. The preparation process flow of the microfluidic sensor unit 150 includes:

step S210: selecting a PMMA plate with the thickness of 1mm, cleaning, drying, and then cutting into a spinning pendent PMMA sheet with the length diameter of 10mm and the short diameter of 3.6mm by using laser to be used as a mold of a micro-flow cavity;

step S220: attaching the spun-draped PMMA sheet to the bottom of the cleaned and dried culture dish by using a double-sided adhesive tape;

step S230: performing PDMS injection molding in a culture dish, uniformly mixing PDMS and a curing agent according to the proportion of 10:1, standing in a vacuum chamber for 20 minutes to remove bubbles, casting into the culture dish, and drying at 80 ℃ for 2 hours to form a PDMS membrane with the thickness of 2-3 mm for preparing a microporous membrane;

step S240: taking out the molded PDMS film, and cutting the PDMS film along the edge of the spun-draping PMMA sheet contained in the PDMS film by using a cutter to form a spun-draping microfluidic cavity;

step S250: cutting the PDMS membrane into a square of 15mm multiplied by 15mm, wherein the center of the micro-flow cavity is aligned with the center of the square;

step S260: drilling holes on the PDMS film at positions corresponding to the top ends of the spinning-hanging micro flow grooves to form an inlet and an outlet, wherein the diameter of each hole is 300-500 mu m;

step S270: adhering a square PDMS film to the front surface of an optical addressing potential sensor with the same size by using an adhesive with stable biological characteristics;

step S280: inserting metal tubes in interference fit with the holes in the inlet and the outlet of the PDMS membrane, wherein the metal tubes are made of high-stability metal (such as stainless steel, Pt and the like), the metal tubes are sleeved with polyethylene microporous tubes and are respectively used as a liquid inlet tube and a liquid outlet tube, and the metal tubes are used as a reference electrode and an auxiliary electrode;

step S290: the wires and working electrodes are led out from the back of the photo-addressable potentiometric sensor and fixed with conductive glue to a cleaned and dried substrate, for example a 1mm thick PMMA plate.

[ light addressable potentiometric sensor ]

As shown in fig. 3, the optical addressing potential sensor 152 of the present embodiment is constituted by an aluminum layer 31, a silicon wafer substrate 32, an insulator layer 33, and a protective layer 34 in this order from the back surface to the front surface. The specific processing flow is as follows:

step S310: selecting an n-type single-side polished silicon wafer, wherein the thickness of the silicon wafer is 500 mu m, cutting the silicon wafer into square silicon single wafers with the thickness of 15mm multiplied by 15mm, cleaning the square silicon single wafers by an RCA standard cleaning process and drying the square silicon single wafers;

photoetching and thinning the corresponding area of the LAPS on the back surface of the silicon single chip by using a 5% hydrofluoric acid wet chemical etching method to form a silicon chip substrate 32 so as to improve the intensity of response current during light excitation;

step S320: growing a layer of SiO 30nm on the corresponding area of LAPS on the front surface of the silicon wafer substrate 32 by a thermal drying oxidation method (the temperature is 1000 ℃ and the time is 40min)₂Forming an insulator layer 33;

step S330: plasma Enhanced Chemical Vapor Deposition (PECVD) method is adopted to deposit SiO₂A silicon nitride layer with the surface of 50nm is formed to be used as a protective layer;

step S340: and forming an aluminum layer with the thickness of 300nm as a conductive layer in the corresponding area of the LAPS on the back surface of the silicon chip substrate through thermal evaporation deposition.

The reference electrode is connected to the signal circuit 120 and cooperates with the auxiliary electrode to provide a dc bias voltage to the photo-addressable potential sensor 152, forming a space charge region at the insulator/semiconductor interface. The exciting light source 130 irradiates the silicon substrate 32 to generate electron-hole pairs, which form a photocurrent under the action of the space charge region electric field, and the photocurrent has an alternating amplitude under the influence of the surface potential. Since the photo-addressable potentiometric sensor 152 is in direct contact with the analysis solution, the surface potential changes, changing the intensity of the photocurrent, and thus achieving quantitative analysis of the substance. The aluminum layer on the back of the photo-addressable potentiometric sensor 152 is connected to the working electrode for outputting a detection signal.

[ Signal Circuit ]

As shown in fig. 4, the signal circuit 120 includes a microcontroller 121 and a LAPS detection circuit 122. The microcontroller 121 adopts a 16-bit MSP426FG4619 processor with low power consumption to realize the control of the detection process. The structure of the LAPS detection circuit 122 includes: I/V conversion circuit 41, zeroing and amplifying circuit 42, low-pass filter circuit 43, impedance circuit 44, and clock circuit 45. In the process of detecting the lap signal, the weak electrical signal generated by the photo-addressable potentiometric sensor 152 is converted from photocurrent to a voltage signal by the I/V conversion circuit 41. The I/V conversion circuit 41 with different gears can be arranged and is matched with an electronic change-over switch to realize optimized detection. The voltage signal is subjected to signal conditioning by the zeroing and amplifying circuit 42, a direct current offset error of the system is removed, in-phase amplification is performed, the signal-to-noise ratio is improved, low-frequency noise is filtered by the low-pass filter circuit 43, the signal quality is improved, and then the signal is sent to the microcontroller 121. The impedance circuit 44 provides a high frequency ac excitation source to the light source modulation circuit 130 for impedance detection, which in combination drives the excitation light source and delivers the detection result to the microprocessor 121 for post-processing. Clock circuit 45 is controlled by microprocessor 121 to provide a stable and reliable clock signal to impedance circuit 44, which can use ADF4001 clock signal chip and 16M active crystal oscillator.

Example 2

As shown in fig. 5, the microfluidic carbohydrate metabolism analysis and detection apparatus 200 of the present embodiment includes: a controller 210, a signal circuit 220, an excitation light source 230, a light source modulation circuit 240, a microfluidic sensor unit 250, a micro-syringe pump 260, a waste reservoir 270, a bubble removal unit 280, and a vacuum pump 290. The liquid inlet channel of the micro-fluidic sensor unit 250 is communicated with the micro-injection pump 260, the liquid outlet channel of the micro-fluidic sensor unit 250 is communicated with the waste liquid pool 270, and the micro-fluidic sensor unit 250 is connected with the signal circuit 220 to realize the bias voltage application and the signal acquisition of the micro-fluidic sensor unit 250. The excitation light source 230 is disposed above the microfluidic sensor unit 250 and connected to the light source modulation circuit 240, so that the microfluidic sensor unit 250 is excited by light to generate a photo-generated current. The light source modulation circuit 240 is connected to the signal circuit 220 and controlled by the signal circuit 220. The signal circuit 220 is connected to the controller 210 via a serial port to perform related control, data transmission and processing functions. The bubble eliminating unit 280 is arranged on the liquid inlet channel between the microfluidic sensor unit 250 and the micro-injection pump 260, and the bubble eliminating unit 280 is communicated with the vacuum pump and used for eliminating micro-bubbles in the detection solution and improving the detection precision.

The micro syringe pump 260 employs a universal asynchronous receive/transmit protocol (UART) to control the syringe pump. The excitation light source 230 is composed of a fixed-focus tunable infrared laser diode module for generating electron-hole pairs. The microfluidic carbohydrate metabolism analysis and detection device 200 of this embodiment has the same structure as that of embodiment 1 except for the bubble elimination unit 280 and the vacuum pump 290, and thus, the description thereof is omitted.

[ bubble eliminating Unit ]

As shown in fig. 6, the bubble removing unit 280 of the present embodiment includes: the vacuum pump comprises an upper substrate 61, a lower substrate 62, a negative pressure cavity 63, a fluid cavity 64, a vacuum pump connecting port 65, a micro-fluid inlet 66, a micro-fluid outlet 67, a vacuum pump connecting pipe 68 and a fluid conduit 69, wherein the negative pressure cavity 63 is communicated with the fluid cavity 64 and is positioned between the upper substrate 61 and the lower substrate 62, the negative pressure cavity 63 is adjacent to the upper substrate 61, the fluid cavity 64 is adjacent to the lower substrate 62, the vacuum pump connecting port 65 is positioned on the upper substrate 61 and is communicated with the negative pressure cavity 63, and the micro-fluid inlet 66 and the micro-fluid outlet 67 are. The specific preparation process of the bubble removing unit 280 of this embodiment is as follows:

step S610: selecting a p-type silicon wafer with the thickness of 540 mu m, cutting to obtain 2 rectangular silicon single wafers with the thickness of 15mm multiplied by 40mm, cleaning by an RCA standard cleaning process and drying;

step S620: etching a silicon wafer by using negative photoresist, etching a circular groove with the radius of 5mm and the depth of 100 mu m on the silicon single chip, and a guide groove with the width of 500 mu m extending along the long side direction of the silicon single chip on two sides of the circular groove, forming a plurality of rows of through holes with different diameters in the circular groove, for example, the through holes comprise 1000 mu m, 500 mu m and 250 mu m, and the distance between every two rows of through holes is equal to the diameter of the through holes; the through holes on the two silicon single sheets are mutually corresponding (the positions, the diameters and the intervals of the through holes are the same), the lengths of the guide grooves are different, the silicon single sheet with the short guide groove is used for forming a negative pressure cavity, and the silicon single sheet with the long guide groove is used for forming a fluid cavity;

step S630: performing PDMS injection molding in a culture dish, uniformly mixing PDMS and a curing agent according to the proportion of 10:1, standing in a vacuum chamber for 20 minutes to remove air bubbles, casting into the culture dish, and drying at 80 ℃ for 2 hours to form a PDMS membrane with the thickness of 200 microns;

step S640: selecting a PMMA plate with the thickness of 1mm, cutting to obtain 2 rectangular pieces with the diameter of 20mm multiplied by 50mm, arranging connecting through holes with the diameter of 500 mu m at positions respectively corresponding to the positions of the 2 silicon single piece guide grooves, arranging fixing through holes matched with the fixing screws at corners, cleaning and drying to obtain an upper substrate 61 and a lower substrate 62;

step S650: the upper substrate 61, the first silicon single chip, the PDMS film, the second silicon single chip, and the lower substrate 62 are sequentially stacked, aligning the through-holes of the first silicon wafer and the second silicon wafer, fixing the through-holes of the upper substrate 61 and the lower substrate 61, ensuring that the coupling through-holes of the upper substrate 61 are aligned with the guide grooves of the first silicon wafer, the coupling through-holes of the lower substrate 62 are aligned with the guide grooves of the second silicon wafer, passing the lead screws through the fixing through-holes of the upper substrate 61 and the lower substrate 62 and fixing them with nuts, so that a negative pressure chamber 63 is formed between the upper substrate 61 and the circular grooves of the first silicon wafer, a fluid cavity 64 is formed between the PDMS film and the circular groove of the second silicon wafer 62, the through hole of the upper substrate 61 is used as a vacuum pump connecting port 65 and is communicated with the negative pressure cavity 63 through the guide groove of the first silicon wafer, and the through holes of the lower substrate 62 are respectively used as a micro-flow inlet 66 and a micro-flow outlet 67 and are communicated with the fluid cavity 64 through the guide groove of the second silicon wafer;

step S660: polyethylene pipes with the diameter of 500 μm are respectively used as a vacuum pump connecting pipe 68 and a fluid conduit 69, sleeved in the vacuum pump connecting port 65, the microflow inlet 66 and the microflow outlet 67, and sealant is coated on the vacuum pump connecting port 65, the microflow inlet 66 and the microflow outlet 67.

The present invention is described in detail with reference to the embodiments, but it can be understood by those skilled in the art that the above embodiments are only one of the preferred embodiments of the present invention, and for space limitation, all embodiments can not be listed herein, and any implementation that can embody the technical solution of the claims of the present invention is within the protection scope of the present invention.

It should be noted that the above is a detailed description of the present invention, and it should not be considered that the present invention is limited to the specific embodiments, and those skilled in the art can make various modifications and variations on the above embodiments without departing from the scope of the present invention.

Claims

1. A microfluidic carbohydrate metabolism analysis assay device, comprising: the device comprises a micro-fluidic sensor unit, a micro-injection pump, a waste liquid pool, a signal circuit, an excitation light source, a light source modulation circuit and a controller;

a liquid inlet channel of the microfluidic sensor unit is communicated with the micro-injection pump, and a liquid outlet channel of the microfluidic sensor unit is communicated with the waste liquid pool;

the signal circuit is connected with the micro-fluidic sensor unit, applies bias voltage to the micro-fluidic sensor unit and collects detection signals from the micro-fluidic sensor unit, and is connected with the light source modulation circuit and sends modulation control signals;

the excitation light source is arranged towards the microfluidic sensor unit, is connected with the light source modulation circuit and emits excitation light for generating photocurrent to the microfluidic sensor unit;

the light source modulation circuit is connected with the excitation light source and modulates the excitation light source to emit excitation light according to the modulation control signal;

the controller is in communication connection with the signal circuit and is used for detecting, controlling, recording and processing collected data.

2. The microfluidic carbohydrate metabolism analysis and detection device according to claim 1, wherein: the micro-fluidic sensor unit comprises a substrate, a light addressing potential sensor, a microporous membrane, a liquid inlet pipe, a liquid outlet pipe and a working electrode;

the conducting layer of the light addressing potential sensor is connected with the working electrode, and the light addressing potential sensor and the working electrode are arranged on the substrate;

the microporous membrane is arranged on the light-addressable potentiometric sensor; has a micro flow groove;

the liquid inlet pipe and the liquid outlet pipe are arranged at two ends of the micro-flow groove and inserted into the microporous membrane.

3. The microfluidic carbohydrate metabolism analysis and detection device according to claim 2, wherein: the micro-flow groove is in a spinning-hanging shape.

4. The microfluidic carbohydrate metabolism analysis and detection device according to claim 2, wherein: the liquid inlet pipe and the liquid outlet pipe are composed of a metal pipe and a polyethylene microporous pipe, the metal pipe is inserted into the microporous film, and the polyethylene microporous pipe is sleeved on the metal pipe.

5. The microfluidic carbohydrate metabolism analysis and detection device according to claim 2, wherein: the optical addressing potential sensor is composed of an aluminum layer, a silicon wafer substrate, an insulator layer and a protective layer in sequence from the back side to the front side.

6. The microfluidic carbohydrate metabolism analysis and detection device according to claim 5, wherein:

the aluminium lamination is connected the working electrode, the protective layer is located the micropore membrane below, the exposure of little chute the protective layer orientation excitation light source sets up.

7. The microfluidic carbohydrate metabolism analysis and detection device according to claim 2, wherein: the signal circuit comprises a microcontroller and a LAPS detection circuit;

the LAPS detection circuit comprises an I/V conversion circuit, a zero setting and amplifying circuit, a low-pass filter circuit, an impedance circuit and a clock circuit; the photocurrent signal of the optical addressing potential sensor is converted into a voltage signal through the I/V conversion circuit; the voltage signal is subjected to error removal and in-phase amplification through the zero setting and amplifying circuit, low-frequency noise is filtered through the low-pass filter circuit, and the low-frequency noise is sent to the microcontroller; the impedance circuit provides a high-frequency alternating current excitation source for the light source modulation circuit to carry out impedance detection and drive an excitation light source; the clock circuit provides a clock signal for the impedance circuit through microprocessor control.

8. The microfluidic carbohydrate metabolism analysis and detection device according to claim 2, wherein: and a bubble eliminating unit is arranged on the liquid inlet channel and is communicated with a vacuum pump.

9. The microfluidic carbohydrate metabolism analysis and detection device according to claim 8, wherein: the bubble eliminating unit comprises an upper substrate, a lower substrate, a negative pressure cavity, a fluid cavity, a vacuum pump connecting port, a micro-flow inlet, a micro-flow outlet, a vacuum pump connecting pipe and a fluid conduit;

the negative pressure cavity is communicated with the fluid cavity, the negative pressure cavity and the fluid cavity are positioned between the upper substrate and the lower substrate, the negative pressure cavity is adjacent to the upper substrate, and the fluid cavity is adjacent to the lower substrate;

the vacuum pump connecting port is positioned on the upper substrate and is communicated with the negative pressure cavity;

the micro-flow inlet and the micro-flow outlet are positioned on the lower substrate and are communicated with the fluid cavity.